Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

33388114 .. 33390844

Gene Structure 2731 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG278100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1041 bp

ATGATTGGTCCCAAAGGTTCAGGGCTGGAGGACTGGGAGCCCTCTTCTCACCACTACGGCTCCTTCTCTGGAGTTTCATGTGACCAGCAATCACGAGTTGTGGCTCTTAACGTGTCCAGTTTGCTTCTGTCTGGCTCGATTCCAGCAGAGATTGGGCTTCTGAACAAGCTCATCAACCTCACCATTGCTGACACCAACTTGACCGGGAGTCTTCCCCTCGAGATGGCCAATCTGACTTCATTAAAGCTTTTGAACATCTCCAACAGCGACCTCACTGGGAGGTTTCCTGGAGAAATCATACTCCGAATGACGGAGCTTGAGGTTCTCGACGCCTACAACAACGACTTTTTAGGGACTCTTCCGCTTGAGATTGCCAGTCTCAAAAAGCTCAGACATCTACATCTTGGCGATAGCTTCTTCACTGGAGAAATTCCCCGAAATTATTCAAATATTCATAGCTTAGAATATTTGGCCCTCAACGATAACTTGCTTACCGGAAAAGTTCCGGCCAGCTTGGCTCGGTTGAAGAATCTCAAGAAACTGTACGTCGGGAATGTTAGAATCTCAATAAACTATAACAAGTTCGACGGTGGAATTCCACCTGAGTTGGGAATGTTGGAGTCGCTGCAAGTGCTTGACATGGTCGGCTGTAAGCTCTCCGGCACGATTCCGACTACTCTGGGCTTTTTGAAGAACTTACACACGTTGAATATATCGAATAACCGTTTGAGTGGTAATATACCTCCAGAACATTCGGATTTGGTAAACCTGATGAGTTTGGACCTCTCTGTTAACAAACTCACCGGTGAAATACCAGAGAGTTTCTCAAAGCTCAAGAACCTTAAACTTCTACAACTAGTTGGGAACAACCTCTACGGCCAGATACCCATCTTCATTGGTGATCTTCCTCATCTGGAGGCGTTTGACGTTGGGGGCAACAACTTTACATTTGCTCTACCGGAGAGTCTTGGCCTGAATGAAAGACTTACGTCCCTCAAGGTATCCGAAAGACAACTCGCAGAGTCTGTTAATCCTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

346

Amino Acids

37.67

Weight (kDa)

5.64

Isoelectric Point (pI)

35.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	46 - 135	1.1e-06	Leucine-rich repeat region
LRR_14	PF23598	124 - 241	1.3e-10	Leucine-rich repeat region
LRR_14	PF23598	200 - 286	9.2e-07	Leucine-rich repeat region
LRR_8	PF13855	232 - 291	3.2e-08	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	362
AcoI	YGGCCR	3 cut(s)	225, 507, 877
AcsI	RAATTY	2 cut(s)	429, 594
AcyI	GRCGYC	1 cut(s)	330
AfaI	GTAC	1 cut(s)	545
AfiI	CCNNNNNNNGG	3 cut(s)	223, 310, 608
AflIII	ACRYGT	2 cut(s)	111, 702
AgeI	ACCGGT	1 cut(s)	803
AgsI	TTSAA	5 cut(s)	253, 447, 526, 691, 709
AhdI	GACNNNNNGTC	1 cut(s)	207
AhlI	ACTAGT	1 cut(s)	856
AjnI	CCWGG	1 cut(s)	286
AluBI	AGCT	9 cut(s)	169, 247, 316, 388, 414, 459, 513, 655, 832
AluI	AGCT	9 cut(s)	169, 247, 316, 388, 414, 459, 513, 655, 832
Alw26I	GTCTC	1 cut(s)	383
AlwNI	CAGNNNCTG	1 cut(s)	1025
Ama87I	CYCGRG	1 cut(s)	218
AoxI	GGCC	5 cut(s)	225, 471, 507, 877, 970
ApeKI	GCWGC	1 cut(s)	625
ApoI	RAATTY	2 cut(s)	429, 594
AsiGI	ACCGGT	1 cut(s)	803
Asp700I	GAANNNNTTC	1 cut(s)	442
AspS9I	GGNCC	3 cut(s)	8, 472, 781
AsuC2I	CCSGG	1 cut(s)	205
AsuHPI	GGTGA	5 cut(s)	41, 172, 793, 818, 911
AvaI	CYCGRG	1 cut(s)	218
AvaII	GGWCC	2 cut(s)	8, 781
BalI	TGGCCA	1 cut(s)	227
BanII	GRGCYC	1 cut(s)	42
BauI	CACGAG	1 cut(s)	93
BbsI	GAAGAC	1 cut(s)	203
BbvI	GCAGC	1 cut(s)	612
BccI	CCATC	2 cut(s)	217, 896
BceAI	ACGGC	2 cut(s)	73, 892
BciT130I	CCWGG	1 cut(s)	288
BciVI	GTATCC	1 cut(s)	1012
BcnI	CCSGG	1 cut(s)	205
BcoDI	GTCTC	1 cut(s)	383
BcuI	ACTAGT	1 cut(s)	856
BfaI	CTAG	1 cut(s)	857
BfuI	GTATCC	1 cut(s)	1012
BglI	GCCNNNNNGGC	1 cut(s)	515
BisI	GCNGC	1 cut(s)	626
BlsI	GCNGC	1 cut(s)	627
Bme1390I	CCNGG	2 cut(s)	205, 288
Bme18I	GGWCC	2 cut(s)	8, 781
BmeRI	GACNNNNNGTC	1 cut(s)	207
BmeT110I	CYCGRG	1 cut(s)	218
BmgT120I	GGNCC	3 cut(s)	8, 472, 781
BmiI	GGNNCC	3 cut(s)	10, 39, 61
BmrFI	CCNGG	2 cut(s)	205, 288
BmrI	ACTGGG	2 cut(s)	43, 285
BmuI	ACTGGG	2 cut(s)	43, 285
BoxI	GACNNNNGTC	1 cut(s)	988
BpiI	GAAGAC	1 cut(s)	203
BplI	GAGNNNNNCTC	2 cut(s)	809, 841
BpmI	CTGGAG	6 cut(s)	47, 90, 309, 444, 729, 935
BpuEI	CTTGAG	5 cut(s)	338, 386, 518, 818, 980
BpuMI	CCSGG	1 cut(s)	205
BsaBI	GATNNNNATC	1 cut(s)	887
BsaHI	GRCGYC	1 cut(s)	330
BsaWI	WCCGGW	3 cut(s)	494, 803, 958
BsaXI	ACNNNNNCTCC	2 cut(s)	44, 74
Bsc4I	CCNNNNNNNGG	3 cut(s)	223, 310, 608
Bse118I	RCCGGY	1 cut(s)	803
Bse1I	ACTGG	5 cut(s)	38, 117, 280, 375, 427
Bse3DI	GCAATG	1 cut(s)	183
Bse8I	GATNNNNATC	1 cut(s)	887
BseBI	CCWGG	1 cut(s)	288
BseJI	GATNNNNATC	1 cut(s)	887
BseLI	CCNNNNNNNGG	3 cut(s)	223, 310, 608
BseMI	GCAATG	1 cut(s)	183
BseMII	CTCAG	2 cut(s)	403, 594
BseNI	ACTGG	5 cut(s)	38, 117, 280, 375, 427
BseXI	GCAGC	1 cut(s)	612
BshFI	GGCC	5 cut(s)	227, 473, 509, 879, 972
BshTI	ACCGGT	1 cut(s)	803
BsiHKCI	CYCGRG	1 cut(s)	218
BsiSI	CCGG	6 cut(s)	204, 495, 506, 660, 804, 959
BslFI	GGGAC	2 cut(s)	367, 976
BslI	CCNNNNNNNGG	3 cut(s)	223, 310, 608
BsmAI	GTCTC	1 cut(s)	383
BsmFI	GGGAC	2 cut(s)	367, 976
BsnI	GGCC	5 cut(s)	227, 473, 509, 879, 972
BsoBI	CYCGRG	1 cut(s)	218
Bsp1286I	GDGCHC	1 cut(s)	42
Bsp143I	GATC	1 cut(s)	901
BspACI	CCGC	1 cut(s)	362
BspANI	GGCC	5 cut(s)	227, 473, 509, 879, 972
BspCNI	CTCAG	2 cut(s)	402, 595
BspLI	GGNNCC	3 cut(s)	10, 39, 61
BsrDI	GCAATG	1 cut(s)	183
BsrFI	RCCGGY	1 cut(s)	803
BsrI	ACTGG	5 cut(s)	38, 117, 280, 375, 427
BssAI	RCCGGY	1 cut(s)	803
BssMI	GATC	1 cut(s)	901
BssNI	GRCGYC	1 cut(s)	330
BssSI	CACGAG	1 cut(s)	93
Bst2BI	CACGAG	1 cut(s)	93
Bst2UI	CCWGG	1 cut(s)	288
Bst4CI	ACNGT	3 cut(s)	543, 590, 725
Bst6I	CTCTTC	2 cut(s)	49, 363
BstACI	GRCGYC	1 cut(s)	330
BstC8I	GCNNGC	1 cut(s)	511
BstDEI	CTNAG	3 cut(s)	389, 460, 603
BstKTI	GATC	1 cut(s)	904
BstMAI	GTCTC	1 cut(s)	383
BstMBI	GATC	1 cut(s)	901
BstMWI	GCNNNNNNNGC	2 cut(s)	515, 631
BstNI	CCWGG	1 cut(s)	288
BstPAI	GACNNNNGTC	1 cut(s)	988
BstSCI	CCNGG	2 cut(s)	203, 286
BstV1I	GCAGC	1 cut(s)	612
BstV2I	GAAGAC	1 cut(s)	203
BsuI	GTATCC	1 cut(s)	1012
BsuRI	GGCC	5 cut(s)	227, 473, 509, 879, 972
BtsIMutI	CAGTG	2 cut(s)	273, 420
Cac8I	GCNNGC	1 cut(s)	511
CaiI	CAGNNNCTG	1 cut(s)	1025
Cfr10I	RCCGGY	1 cut(s)	803
Cfr13I	GGNCC	3 cut(s)	8, 472, 781
CseI	GACGC	1 cut(s)	338
Csp6I	GTAC	1 cut(s)	544
CspAI	ACCGGT	1 cut(s)	803
CspCI	CAANNNNNGTGG	2 cut(s)	588, 623
CviAII	CATG	3 cut(s)	78, 640, 1038
CviQI	GTAC	1 cut(s)	544
DdeI	CTNAG	3 cut(s)	389, 460, 603
DpnI	GATC	1 cut(s)	903
DpnII	GATC	1 cut(s)	901
DriI	GACNNNNNGTC	1 cut(s)	207
EaeI	YGGCCR	3 cut(s)	225, 507, 877
Eam1104I	CTCTTC	2 cut(s)	49, 363
Eam1105I	GACNNNNNGTC	1 cut(s)	207
EarI	CTCTTC	2 cut(s)	49, 363
Eco24I	GRGCYC	1 cut(s)	42
Eco47I	GGWCC	2 cut(s)	8, 781
Eco88I	CYCGRG	1 cut(s)	218
EcoRI	GAATTC	1 cut(s)	594
EcoRII	CCWGG	1 cut(s)	286
EcoT38I	GRGCYC	1 cut(s)	42
FaeI	CATG	3 cut(s)	81, 643, 1041
FaiI	YATR	8 cut(s)	79, 299, 456, 576, 641, 713, 740, 1039
FaqI	GGGAC	2 cut(s)	367, 976
FatI	CATG	3 cut(s)	77, 639, 1037
Fnu4HI	GCNGC	1 cut(s)	626
FriOI	GRGCYC	1 cut(s)	42
Fsp4HI	GCNGC	1 cut(s)	626
FspBI	CTAG	1 cut(s)	857
GluI	GCNGC	1 cut(s)	626
GsuI	CTGGAG	6 cut(s)	47, 90, 309, 444, 729, 935
HaeIII	GGCC	5 cut(s)	227, 473, 509, 879, 972
HapII	CCGG	6 cut(s)	204, 495, 506, 660, 804, 959
HgaI	GACGC	1 cut(s)	338
Hin1I	GRCGYC	1 cut(s)	330
Hin1II	CATG	3 cut(s)	81, 643, 1041
HincII	GTYRAC	1 cut(s)	793
HindII	GTYRAC	1 cut(s)	793
HindIII	AAGCTT	1 cut(s)	245
HinfI	GANTC	9 cut(s)	139, 208, 355, 529, 561, 620, 667, 964, 1022
HpaI	GTTAAC	1 cut(s)	793
HpaII	CCGG	6 cut(s)	204, 495, 506, 660, 804, 959
HphI	GGTGA	5 cut(s)	41, 172, 793, 818, 911
Hpy166II	GTNNAC	2 cut(s)	766, 793
Hpy188I	TCNGA	7 cut(s)	162, 234, 305, 392, 672, 757, 1006
Hpy188III	TCNNGA	9 cut(s)	69, 93, 220, 326, 535, 550, 746, 835, 914
Hpy8I	GTNNAC	2 cut(s)	766, 793
Hpy99I	CGWCG	3 cut(s)	332, 551, 590
HpyAV	CCTTC	1 cut(s)	73
HpyCH4III	ACNGT	3 cut(s)	543, 590, 725
HpyCH4IV	ACGT	5 cut(s)	111, 546, 704, 927, 989
HpyCH4V	TGCA	2 cut(s)	628, 1037
HpyF10VI	GCNNNNNNNGC	2 cut(s)	515, 631
HpyF3I	CTNAG	3 cut(s)	389, 460, 603
HpySE526I	ACGT	5 cut(s)	111, 546, 704, 927, 989
Hsp92I	GRCGYC	1 cut(s)	330
Hsp92II	CATG	3 cut(s)	81, 643, 1041
KspAI	GTTAAC	1 cut(s)	793
Kzo9I	GATC	1 cut(s)	901
LmnI	GCTCC	3 cut(s)	37, 65, 313
Lsp1109I	GCAGC	1 cut(s)	612
MaeI	CTAG	1 cut(s)	857
MaeII	ACGT	5 cut(s)	111, 546, 704, 927, 989
MaeIII	GTNAC	1 cut(s)	80
MalI	GATC	1 cut(s)	903
MboI	GATC	1 cut(s)	901
MboII	GAAGA	8 cut(s)	36, 203, 350, 409, 538, 703, 883, 896
MhlI	GDGCHC	1 cut(s)	42
MlsI	TGGCCA	1 cut(s)	227
MluCI	AATT	3 cut(s)	429, 439, 594
MluNI	TGGCCA	1 cut(s)	227
MlyI	GAGTC	5 cut(s)	217, 349, 629, 973, 1031
MmeI	TCCRAC	3 cut(s)	285, 597, 695
Mox20I	TGGCCA	1 cut(s)	227
MroXI	GAANNNNTTC	1 cut(s)	442
MscI	TGGCCA	1 cut(s)	227
MseI	TTAA	5 cut(s)	108, 242, 792, 843, 1029
Msp20I	TGGCCA	1 cut(s)	227
MspI	CCGG	6 cut(s)	204, 495, 506, 660, 804, 959
MspR9I	CCNGG	2 cut(s)	205, 288
MvaI	CCWGG	1 cut(s)	288
MwoI	GCNNNNNNNGC	2 cut(s)	515, 631
NciI	CCSGG	1 cut(s)	205
NdeII	GATC	1 cut(s)	901
NlaIII	CATG	3 cut(s)	81, 643, 1041
NlaIV	GGNNCC	3 cut(s)	10, 39, 61
NmuCI	GTSAC	1 cut(s)	80
PaeR7I	CTCGAG	1 cut(s)	218
PdmI	GAANNNNTTC	1 cut(s)	442
PfeI	GAWTC	4 cut(s)	139, 529, 561, 667
PflFI	GACNNNGTC	1 cut(s)	641
PfoI	TCCNGGA	1 cut(s)	286
PinAI	ACCGGT	1 cut(s)	803
PkrI	GCNGC	1 cut(s)	627
PleI	GAGTC	5 cut(s)	216, 349, 628, 972, 1030
PpsI	GAGTC	5 cut(s)	216, 349, 628, 972, 1030
PshAI	GACNNNNGTC	1 cut(s)	988
Psp6I	CCWGG	1 cut(s)	286
PspGI	CCWGG	1 cut(s)	286
PspN4I	GGNNCC	3 cut(s)	10, 39, 61
PspPI	GGNCC	3 cut(s)	8, 472, 781
PsrI	GAACNNNNNNTAC	2 cut(s)	857, 889
PstNI	CAGNNNCTG	1 cut(s)	1025
PsyI	GACNNNGTC	1 cut(s)	641
RsaI	GTAC	1 cut(s)	545
RsaNI	GTAC	1 cut(s)	544
SaqAI	TTAA	5 cut(s)	108, 242, 792, 843, 1029
SatI	GCNGC	1 cut(s)	626
Sau3AI	GATC	1 cut(s)	901
Sau96I	GGNCC	3 cut(s)	8, 472, 781
SchI	GAGTC	5 cut(s)	217, 349, 629, 973, 1031
ScrFI	CCNGG	2 cut(s)	205, 288
SduI	GDGCHC	1 cut(s)	42
Sfr274I	CTCGAG	1 cut(s)	218
SgrAI	CRCCGGYG	1 cut(s)	803
SinI	GGWCC	2 cut(s)	8, 781
SlaI	CTCGAG	1 cut(s)	218
SmlI	CTYRAG	6 cut(s)	218, 317, 365, 533, 833, 995
SmoI	CTYRAG	6 cut(s)	218, 317, 365, 533, 833, 995
SpeI	ACTAGT	1 cut(s)	856
Sse9I	AATT	3 cut(s)	429, 439, 594
SsiI	CCGC	1 cut(s)	362
SspI	AATATT	2 cut(s)	451, 467
SspMI	CTAG	1 cut(s)	857
StyD4I	CCNGG	2 cut(s)	203, 286
TaaI	ACNGT	3 cut(s)	543, 590, 725
TaiI	ACGT	5 cut(s)	114, 549, 707, 930, 992
TaqI	TCGA	5 cut(s)	137, 219, 327, 585, 716
TasI	AATT	3 cut(s)	429, 439, 594
TfiI	GAWTC	4 cut(s)	139, 529, 561, 667
Tru1I	TTAA	5 cut(s)	108, 242, 792, 843, 1029
Tru9I	TTAA	5 cut(s)	108, 242, 792, 843, 1029
TscAI	CASTG	2 cut(s)	280, 427
TseFI	GTSAC	1 cut(s)	80
TseI	GCWGC	1 cut(s)	625
Tsp45I	GTSAC	1 cut(s)	80
TspDTI	ATGAA	5 cut(s)	66, 228, 443, 883, 993
TspGWI	ACGGA	1 cut(s)	326
TspRI	CASTG	2 cut(s)	280, 427
Tth111I	GACNNNGTC	1 cut(s)	641
VpaK11BI	GGWCC	2 cut(s)	8, 781
XapI	RAATTY	2 cut(s)	429, 594
XhoI	CTCGAG	1 cut(s)	218
XmnI	GAANNNNTTC	1 cut(s)	442
XspI	CTAG	1 cut(s)	857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.