Belongs to the protein kinase superfamily. Ser Thr protein kinase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 758 |
| AccII | CGCG | 2 cut(s) | 684, 686 |
| AccIII | TCCGGA | 1 cut(s) | 1084 |
| AciI | CCGC | 4 cut(s) | 58, 146, 408, 570 |
| AclWI | GGATC | 3 cut(s) | 32, 45, 330 |
| AcoI | YGGCCR | 3 cut(s) | 225, 624, 952 |
| AcsI | RAATTY | 2 cut(s) | 429, 576 |
| AcuI | CTGAAG | 2 cut(s) | 1076, 1153 |
| AcyI | GRCGYC | 2 cut(s) | 330, 755 |
| AfaI | GTAC | 1 cut(s) | 545 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 131, 223, 590, 713 |
| AflIII | ACRYGT | 1 cut(s) | 111 |
| AgsI | TTSAA | 4 cut(s) | 253, 526, 908, 1042 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 254, 286, 531, 563 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1052, 1084 |
| Alw21I | GWGCWC | 2 cut(s) | 372, 816 |
| Alw26I | GTCTC | 1 cut(s) | 809 |
| AlwI | GGATC | 3 cut(s) | 32, 45, 330 |
| Ama87I | CYCGRG | 2 cut(s) | 218, 1014 |
| Aor13HI | TCCGGA | 1 cut(s) | 1084 |
| AoxI | GGCC | 6 cut(s) | 7, 225, 472, 624, 859, 952 |
| ApoI | RAATTY | 2 cut(s) | 429, 576 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 416, 1067 |
| AspLEI | GCGC | 1 cut(s) | 686 |
| AspS9I | GGNCC | 4 cut(s) | 8, 353, 472, 860 |
| AsuC2I | CCSGG | 2 cut(s) | 288, 1003 |
| AsuHPI | GGTGA | 5 cut(s) | 484, 631, 757, 775, 991 |
| AvaI | CYCGRG | 2 cut(s) | 218, 1014 |
| AvaII | GGWCC | 1 cut(s) | 353 |
| BalI | TGGCCA | 2 cut(s) | 227, 626 |
| BamHI | GGATCC | 1 cut(s) | 37 |
| BanII | GRGCYC | 3 cut(s) | 372, 492, 816 |
| BauI | CACGAG | 1 cut(s) | 93 |
| BbsI | GAAGAC | 2 cut(s) | 203, 1049 |
| Bbv12I | GWGCWC | 2 cut(s) | 372, 816 |
| BbvCI | CCTCAGC | 1 cut(s) | 1121 |
| BccI | CCATC | 2 cut(s) | 217, 592 |
| BceAI | ACGGC | 1 cut(s) | 874 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1005, 1039 |
| BcnI | CCSGG | 2 cut(s) | 288, 1003 |
| BcoDI | GTCTC | 1 cut(s) | 809 |
| BfaI | CTAG | 3 cut(s) | 26, 662, 1112 |
| BfmI | CTRYAG | 1 cut(s) | 695 |
| BglII | AGATCT | 1 cut(s) | 763 |
| Bme1390I | CCNGG | 2 cut(s) | 288, 1003 |
| Bme18I | GGWCC | 1 cut(s) | 353 |
| BmeT110I | CYCGRG | 2 cut(s) | 218, 1014 |
| BmgT120I | GGNCC | 4 cut(s) | 8, 353, 472, 860 |
| BmiI | GGNNCC | 2 cut(s) | 10, 39 |
| BmrFI | CCNGG | 2 cut(s) | 288, 1003 |
| BmrI | ACTGGG | 1 cut(s) | 43 |
| BmsI | GCATC | 1 cut(s) | 409 |
| BmuI | ACTGGG | 1 cut(s) | 43 |
| BpiI | GAAGAC | 2 cut(s) | 203, 1049 |
| BpmI | CTGGAG | 3 cut(s) | 90, 444, 917 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1121 |
| BpuEI | CTTGAG | 3 cut(s) | 338, 518, 800 |
| BpuMI | CCSGG | 2 cut(s) | 288, 1003 |
| BsaHI | GRCGYC | 2 cut(s) | 330, 755 |
| BsaWI | WCCGGW | 5 cut(s) | 361, 494, 652, 785, 1084 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1052, 1082 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 131, 223, 590, 713 |
| Bse118I | RCCGGY | 1 cut(s) | 641 |
| Bse1I | ACTGG | 5 cut(s) | 38, 117, 355, 427, 1066 |
| Bse3DI | GCAATG | 1 cut(s) | 183 |
| BseAI | TCCGGA | 1 cut(s) | 1084 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 131, 223, 590, 713 |
| BseMI | GCAATG | 1 cut(s) | 183 |
| BseMII | CTCAG | 5 cut(s) | 316, 576, 822, 933, 1135 |
| BseNI | ACTGG | 5 cut(s) | 38, 117, 355, 427, 1066 |
| Bsh1236I | CGCG | 2 cut(s) | 684, 686 |
| Bsh1285I | CGRYCG | 1 cut(s) | 58 |
| BshFI | GGCC | 6 cut(s) | 9, 227, 474, 626, 861, 954 |
| BsiEI | CGRYCG | 1 cut(s) | 58 |
| BsiHKAI | GWGCWC | 2 cut(s) | 372, 816 |
| BsiHKCI | CYCGRG | 2 cut(s) | 218, 1014 |
| BslFI | GGGAC | 1 cut(s) | 1162 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 131, 223, 590, 713 |
| BsmAI | GTCTC | 1 cut(s) | 809 |
| BsmFI | GGGAC | 1 cut(s) | 1162 |
| BsnI | GGCC | 6 cut(s) | 9, 227, 474, 626, 861, 954 |
| BsoBI | CYCGRG | 2 cut(s) | 218, 1014 |
| Bsp1286I | GDGCHC | 3 cut(s) | 372, 492, 816 |
| Bsp13I | TCCGGA | 1 cut(s) | 1084 |
| Bsp143I | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| BspACI | CCGC | 4 cut(s) | 58, 146, 408, 570 |
| BspANI | GGCC | 6 cut(s) | 9, 227, 474, 626, 861, 954 |
| BspCNI | CTCAG | 5 cut(s) | 315, 577, 821, 934, 1134 |
| BspEI | TCCGGA | 1 cut(s) | 1084 |
| BspFNI | CGCG | 2 cut(s) | 684, 686 |
| BspLI | GGNNCC | 2 cut(s) | 10, 39 |
| BspPI | GGATC | 3 cut(s) | 32, 45, 330 |
| BsrDI | GCAATG | 1 cut(s) | 183 |
| BsrFI | RCCGGY | 1 cut(s) | 641 |
| BsrI | ACTGG | 5 cut(s) | 38, 117, 355, 427, 1066 |
| BssAI | RCCGGY | 1 cut(s) | 641 |
| BssMI | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| BssNI | GRCGYC | 2 cut(s) | 330, 755 |
| BssSI | CACGAG | 1 cut(s) | 93 |
| Bst2BI | CACGAG | 1 cut(s) | 93 |
| Bst4CI | ACNGT | 1 cut(s) | 482 |
| Bst6I | CTCTTC | 1 cut(s) | 49 |
| BstACI | GRCGYC | 2 cut(s) | 330, 755 |
| BstC8I | GCNNGC | 3 cut(s) | 137, 372, 628 |
| BstDEI | CTNAG | 5 cut(s) | 302, 585, 808, 942, 1121 |
| BstFNI | CGCG | 2 cut(s) | 684, 686 |
| BstHHI | GCGC | 1 cut(s) | 686 |
| BstKTI | GATC | 5 cut(s) | 6, 40, 325, 766, 886 |
| BstMAI | GTCTC | 1 cut(s) | 809 |
| BstMBI | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| BstMCI | CGRYCG | 1 cut(s) | 58 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 414 |
| BstSCI | CCNGG | 2 cut(s) | 286, 1001 |
| BstSFI | CTRYAG | 1 cut(s) | 695 |
| BstUI | CGCG | 2 cut(s) | 684, 686 |
| BstV2I | GAAGAC | 2 cut(s) | 203, 1049 |
| BstX2I | RGATCY | 3 cut(s) | 37, 322, 763 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1073 |
| BstYI | RGATCY | 3 cut(s) | 37, 322, 763 |
| BsuRI | GGCC | 6 cut(s) | 9, 227, 474, 626, 861, 954 |
| BtsIMutI | CAGTG | 4 cut(s) | 280, 348, 420, 718 |
| Cac8I | GCNNGC | 3 cut(s) | 137, 372, 628 |
| CfoI | GCGC | 1 cut(s) | 686 |
| Cfr10I | RCCGGY | 1 cut(s) | 641 |
| Cfr13I | GGNCC | 4 cut(s) | 8, 353, 472, 860 |
| CseI | GACGC | 1 cut(s) | 338 |
| Csp6I | GTAC | 1 cut(s) | 544 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 570, 605 |
| CviAII | CATG | 4 cut(s) | 78, 622, 1126, 1190 |
| CviQI | GTAC | 1 cut(s) | 544 |
| DdeI | CTNAG | 5 cut(s) | 302, 585, 808, 942, 1121 |
| DpnI | GATC | 5 cut(s) | 5, 39, 324, 765, 885 |
| DpnII | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| DraI | TTTAAA | 2 cut(s) | 243, 774 |
| EaeI | YGGCCR | 3 cut(s) | 225, 624, 952 |
| Eam1104I | CTCTTC | 1 cut(s) | 49 |
| EarI | CTCTTC | 1 cut(s) | 49 |
| EciI | GGCGGA | 1 cut(s) | 161 |
| Ecl136II | GAGCTC | 2 cut(s) | 370, 814 |
| Eco24I | GRGCYC | 3 cut(s) | 372, 492, 816 |
| Eco47I | GGWCC | 1 cut(s) | 353 |
| Eco53kI | GAGCTC | 2 cut(s) | 370, 814 |
| Eco57I | CTGAAG | 2 cut(s) | 1076, 1153 |
| Eco88I | CYCGRG | 2 cut(s) | 218, 1014 |
| EcoICRI | GAGCTC | 2 cut(s) | 370, 814 |
| EcoRI | GAATTC | 1 cut(s) | 576 |
| EcoT38I | GRGCYC | 3 cut(s) | 372, 492, 816 |
| FaeI | CATG | 4 cut(s) | 81, 625, 1129, 1193 |
| FaqI | GGGAC | 1 cut(s) | 1162 |
| FatI | CATG | 4 cut(s) | 77, 621, 1125, 1189 |
| FauI | CCCGC | 1 cut(s) | 401 |
| FriOI | GRGCYC | 3 cut(s) | 372, 492, 816 |
| FspBI | CTAG | 3 cut(s) | 26, 662, 1112 |
| GlaI | GCGC | 1 cut(s) | 685 |
| GsuI | CTGGAG | 3 cut(s) | 90, 444, 917 |
| HaeIII | GGCC | 6 cut(s) | 9, 227, 474, 626, 861, 954 |
| HgaI | GACGC | 1 cut(s) | 338 |
| HhaI | GCGC | 1 cut(s) | 686 |
| Hin1I | GRCGYC | 2 cut(s) | 330, 755 |
| Hin1II | CATG | 4 cut(s) | 81, 625, 1129, 1193 |
| Hin6I | GCGC | 1 cut(s) | 684 |
| HinP1I | GCGC | 1 cut(s) | 684 |
| HincII | GTYRAC | 2 cut(s) | 175, 1117 |
| HindII | GTYRAC | 2 cut(s) | 175, 1117 |
| HinfI | GANTC | 9 cut(s) | 208, 451, 529, 602, 649, 748, 946, 1051, 1164 |
| HphI | GGTGA | 5 cut(s) | 484, 631, 757, 775, 991 |
| Hpy166II | GTNNAC | 3 cut(s) | 175, 356, 1117 |
| Hpy188I | TCNGA | 7 cut(s) | 162, 305, 435, 448, 753, 811, 1056 |
| Hpy188III | TCNNGA | 9 cut(s) | 69, 93, 220, 250, 326, 817, 896, 1016, 1085 |
| Hpy8I | GTNNAC | 3 cut(s) | 175, 356, 1117 |
| Hpy99I | CGWCG | 1 cut(s) | 332 |
| HpyAV | CCTTC | 4 cut(s) | 73, 516, 957, 1051 |
| HpyCH4III | ACNGT | 1 cut(s) | 482 |
| HpyCH4IV | ACGT | 4 cut(s) | 111, 546, 755, 1046 |
| HpyCH4V | TGCA | 5 cut(s) | 400, 610, 1026, 1104, 1187 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 414 |
| HpyF3I | CTNAG | 5 cut(s) | 302, 585, 808, 942, 1121 |
| HpySE526I | ACGT | 4 cut(s) | 111, 546, 755, 1046 |
| Hsp92I | GRCGYC | 2 cut(s) | 330, 755 |
| Hsp92II | CATG | 4 cut(s) | 81, 625, 1129, 1193 |
| HspAI | GCGC | 1 cut(s) | 684 |
| Kpn2I | TCCGGA | 1 cut(s) | 1084 |
| Kzo9I | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| LweI | GCATC | 1 cut(s) | 409 |
| MaeI | CTAG | 3 cut(s) | 26, 662, 1112 |
| MaeII | ACGT | 4 cut(s) | 111, 546, 755, 1046 |
| MaeIII | GTNAC | 4 cut(s) | 80, 295, 551, 632 |
| MalI | GATC | 5 cut(s) | 5, 39, 324, 765, 885 |
| MboI | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| MboII | GAAGA | 7 cut(s) | 36, 203, 291, 538, 878, 1054, 1159 |
| MflI | RGATCY | 3 cut(s) | 37, 322, 763 |
| MhlI | GDGCHC | 3 cut(s) | 372, 492, 816 |
| MlsI | TGGCCA | 2 cut(s) | 227, 626 |
| MluCI | AATT | 6 cut(s) | 412, 429, 439, 539, 576, 1080 |
| MluNI | TGGCCA | 2 cut(s) | 227, 626 |
| MlyI | GAGTC | 4 cut(s) | 217, 611, 757, 955 |
| MmeI | TCCRAC | 1 cut(s) | 285 |
| Mox20I | TGGCCA | 2 cut(s) | 227, 626 |
| MroI | TCCGGA | 1 cut(s) | 1084 |
| MroXI | GAANNNNTTC | 2 cut(s) | 416, 1067 |
| MscI | TGGCCA | 2 cut(s) | 227, 626 |
| MseI | TTAA | 3 cut(s) | 242, 702, 773 |
| Msp20I | TGGCCA | 2 cut(s) | 227, 626 |
| MspA1I | CMGCKG | 1 cut(s) | 630 |
| MspR9I | CCNGG | 2 cut(s) | 288, 1003 |
| MvnI | CGCG | 2 cut(s) | 684, 686 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 414 |
| NciI | CCSGG | 2 cut(s) | 288, 1003 |
| NdeII | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| NlaIII | CATG | 4 cut(s) | 81, 625, 1129, 1193 |
| NlaIV | GGNNCC | 2 cut(s) | 10, 39 |
| NmuCI | GTSAC | 2 cut(s) | 80, 295 |
| PaeR7I | CTCGAG | 2 cut(s) | 218, 1014 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 602, 984 |
| PdmI | GAANNNNTTC | 2 cut(s) | 416, 1067 |
| PfeI | GAWTC | 5 cut(s) | 451, 529, 649, 1051, 1164 |
| PfoI | TCCNGGA | 1 cut(s) | 286 |
| PleI | GAGTC | 4 cut(s) | 216, 610, 756, 954 |
| PpsI | GAGTC | 4 cut(s) | 216, 610, 756, 954 |
| Psp124BI | GAGCTC | 2 cut(s) | 372, 816 |
| PspN4I | GGNNCC | 2 cut(s) | 10, 39 |
| PspPI | GGNCC | 4 cut(s) | 8, 353, 472, 860 |
| PsuI | RGATCY | 3 cut(s) | 37, 322, 763 |
| PvuII | CAGCTG | 1 cut(s) | 630 |
| RsaI | GTAC | 1 cut(s) | 545 |
| RsaNI | GTAC | 1 cut(s) | 544 |
| SacI | GAGCTC | 2 cut(s) | 372, 816 |
| SaqAI | TTAA | 3 cut(s) | 242, 702, 773 |
| Sau3AI | GATC | 5 cut(s) | 3, 37, 322, 763, 883 |
| Sau96I | GGNCC | 4 cut(s) | 8, 353, 472, 860 |
| SchI | GAGTC | 4 cut(s) | 217, 611, 757, 955 |
| ScrFI | CCNGG | 2 cut(s) | 288, 1003 |
| SduI | GDGCHC | 3 cut(s) | 372, 492, 816 |
| SfaNI | GCATC | 1 cut(s) | 409 |
| SfcI | CTRYAG | 1 cut(s) | 695 |
| Sfr274I | CTCGAG | 2 cut(s) | 218, 1014 |
| SinI | GGWCC | 1 cut(s) | 353 |
| SlaI | CTCGAG | 2 cut(s) | 218, 1014 |
| SmlI | CTYRAG | 5 cut(s) | 218, 317, 533, 815, 1014 |
| SmoI | CTYRAG | 5 cut(s) | 218, 317, 533, 815, 1014 |
| Sse9I | AATT | 6 cut(s) | 412, 429, 439, 539, 576, 1080 |
| SsiI | CCGC | 4 cut(s) | 58, 146, 408, 570 |
| SspMI | CTAG | 3 cut(s) | 26, 662, 1112 |
| SstI | GAGCTC | 2 cut(s) | 372, 816 |
| StyD4I | CCNGG | 2 cut(s) | 286, 1001 |
| TaaI | ACNGT | 1 cut(s) | 482 |
| TaiI | ACGT | 4 cut(s) | 114, 549, 758, 1049 |
| TaqI | TCGA | 4 cut(s) | 219, 327, 978, 1015 |
| TasI | AATT | 6 cut(s) | 412, 429, 439, 539, 576, 1080 |
| TfiI | GAWTC | 5 cut(s) | 451, 529, 649, 1051, 1164 |
| Tru1I | TTAA | 3 cut(s) | 242, 702, 773 |
| Tru9I | TTAA | 3 cut(s) | 242, 702, 773 |
| TscAI | CASTG | 4 cut(s) | 280, 355, 427, 718 |
| TseFI | GTSAC | 2 cut(s) | 80, 295 |
| Tsp45I | GTSAC | 2 cut(s) | 80, 295 |
| TspDTI | ATGAA | 5 cut(s) | 66, 443, 865, 997, 1145 |
| TspRI | CASTG | 4 cut(s) | 280, 355, 427, 718 |
| VpaK11BI | GGWCC | 1 cut(s) | 353 |
| XapI | RAATTY | 2 cut(s) | 429, 576 |
| XhoI | CTCGAG | 2 cut(s) | 218, 1014 |
| XmnI | GAANNNNTTC | 2 cut(s) | 416, 1067 |
| XspI | CTAG | 3 cut(s) | 26, 662, 1112 |
| ZraI | GACGTC | 1 cut(s) | 756 |