Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

31296989 .. 31300045

Gene Structure 3057 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ49175

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2892 bp

ATGGTACCGGTTGGTTCCCCTTTTTGGCATAATCTCCTCCACATTTCTTTCATTTTCCTTTTGCTTCCACAATGTTGTTTCTGCGTTAGTGAACTTGACGCACTGTTGATGCTCAAGAAAGCCATGATTGGTCCCAAAGGTTCAGGGCTAGAGGACTGGGAGCCCTCTTCTCACCACTACTGCTCCTTCTTTGGAGTTTCATGTGACCAGCAATCACGAGTTGTTGCTCTCAACTTGTCCGGTTTGCCTCTGTTTGGCTCGATTCCGGTGGAGATTGGGCTTCTGAACAAGCTCGTCAACCTCACCATTGCTGAAGACAACTTGACAGGGAGTCTTCCCCTGGAGATCGCCAACCTGACTTCTTTAAAGCTCTTGAACATCTCCAACAACTTGTTCAGTGGAAGATTTCCCGGAGAAGTCACGCTCAGAATGACAGAGCTTGAGGTTCTCGACGCGTACGACAACGACTTTATTGGGAATCTTCCCCTTGAGATCGCCAGTCTTAGAAGGCTCAAACATCTACATCTTGGCGGGAACTTCTTCACTGGAGAAATTCCCGGAAATTATTCAAAGATTCAAAGCTTAGAATATTTGGCCCTCAACGCTAATTCGCTTGCCGGAAAATTTCCGGCCAGCTTGGCTCGGTTGAGGAATCTCAAGGAATTGTACGTCGGGTACTATAACAATTTCGACGGTGGAATTCCACCTGAGTTGGGATCGTTGAGGTCGCTGCAAGTTCTAGACATGGCCAGCTGTGGCCTCTCCGGCACGATACCGACGACTATGGGCTTTTTGAAGAACGTACACTCGTTGTTTTTAAAGGCTAACCATTTCAGTGGTAATATACCTCCAGAACTTTCGGGTATGGCAAGCCTGATGAGTTTGACCCTCTCTATAAATGAACTCACCGGTGAAATACCAAAGAGTTTCTCAAAGCTCAAGAACCTTACACTTCTCAAGCTGAATGATAACAACCTCTACGGCCAGATACCCAACTTCATTGGTGATCTTCCTCATCTGGAGGTGTTTAGCGTCGGGAACAACAACTTTACATTTGCTCTACCGGAGAGTCTTGGCCGGAATGAAAGACTTATGTACCTCAATGTAACCGGTAACCACCTCACCGGGTTGATTCCTCGTTATTTGTGCAAAGGAGGGAGGTTGAGGAAGTTGATTCTAATGGAGAACCACTTCTCTGGGCCGATTCCGGAAGCATTGGGCCGGTGCAAGTCGCTCATTGAAATCAGATTGATGAAAAACACACTCAACGGGACTATTCCTGCTGGAATCTTGAGCTTTCCCGATGTGATCATTATTGAGCTCAACGATAACCACTTATATGGAGAACTTCCGTTGCAGATGTCTGCTCCCTCACTCGAAATCCTCACACTTTCCCGGAATCATATTTCTGGAACAATTCCGTCCTCCGTTGGAACATTCAAGAATTTACAGACTCTTTCGCTGGATATGAACAGATTCTCCGGGCAAGTTCCAAAAGAAATCTTCAGTTTACAATCGTTAAACAGACTCGACATGAGCAGCAACAAATTCGGTGGTGAAATTCCGGCTGCAATTTCTGATTGTTCCTCTCTAACGTCCCTAGATTTAAGTCAAAACACTTTGGTTGGGGAAATCCCAAGAGGGATAGGCAAACTGAAAGTAATTGAGTTTCTTAATTTCTCAGGGAACAAGCTAACAGGTCCAATACCTGTGGAATTCAGATACATGGCGAGCCTCACAACTTTGGATCTCTCCTATAACAATTTCACTGGTACGAATCCAACCGAAGGAAATCCCAATCTGTGTTCGCCCAGAAAGGTGTTCTGCAGCTCCTATAACCACCCACGACCTGGAGACCATAAAGTCATCATCGCTGGACTCACTGGAGCTCTGCTAACTTTGCTACTCATGTCGCTTCTCATATATGTACTTAGCTGGGTTTATAGGAAGAGAAAGATCAGAATTATTGAGAAATGTGGCTCTTGGAAGCTCACAGTTTTCCAAGTACAGCTTGATCTCAATATAGAGGACGTTCTCCAGTGTCTAAACGCGGAGAACATTATCGGCAGGGGCGGTGCTGGAGTTGTCTACAAGGGGACAATGCCAAACGGTGGTGATGTCGCCATCAAACGGTTGAAGAGGGATCAGGGATTTTCGGCTGAAATCAAAACATTGGGACAAATCAAGCACCGGAATATAGTGAGGCTGTTGGGGTACATGTCAAACAAGGACACAAATTTGTTGCTGTACGAGTACATGCCTAATGGTAGCTTGGGACAACTTTTGCATGGGTCTAACGGAAGGCATTTGGAGTGGGAAATGAGGTACAAGATTGCTGTGGAAGCTGCAAAGGGATTGTGTTATCTCCACCATGATTGCTCGCCTCTGATTATACACAGGGATATAAAGTCCTATAACATCTTGCTGGACTCTAATTTCGAAGCCCATATTGCTGATTTCGGACTTGCCAAGTACTTTCAAGGACCAACAGATTGCATGTCTTCGTTTGCTGGTACCTTCGGTTACATTGCCCCAGAGTACGGTCACACAATGAAAGTGGATGAGAAAATTGATGTGTATAGCTTTGGTGTGGTTCTGCTGGAACTAATAACAGGGAGGAAACCAGTGGTGGAACTAGTGGAAGAAGTTATGAACATAGTAAGTTGGGTAAGGAAAACCACATCAGAAATCCCTCAGTCATCCACTACATCATCTGTACTCACAGTAGTGGACTCCAGGCTCAGTGGGTTCCCACTGGCAAGTGTCGAGCATGTGTTCAAGATAGCAATGATGTGCGTTGACAATCACAGCCCTGCAAGACCTACGATGAGGGAAGTCGTCTACTTCCTCACTAATCCTCCTAGTTCAGCTCCAGGCTTGACAAACCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

963

Amino Acids

106.22

Weight (kDa)

7.57

Isoelectric Point (pI)

38.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 69	9.1e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	164 - 273	1e-07	Leucine-rich repeat region
LRR_8	PF13855	457 - 517	6.2e-07	Leucine rich repeat
LRR_8	PF13855	482 - 541	1.1e-07	Leucine rich repeat
LRR_8	PF13855	530 - 589	2.9e-07	Leucine rich repeat
Pkinase	PF00069	684 - 946	6.2e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	684 - 948	3.3e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1862
Acc65I	GGTACC	2 cut(s)	4, 2513
AccB1I	GGYRCC	2 cut(s)	4, 2513
AccB7I	CCANNNNNTGG	2 cut(s)	1850, 2111
AccI	GTMKAC	2 cut(s)	2088, 2841
AccII	CGCG	2 cut(s)	455, 2051
AccIII	TCCGGA	1 cut(s)	1207
AciI	CCGC	3 cut(s)	531, 2051, 2073
AclWI	GGATC	3 cut(s)	724, 1755, 2151
AcoI	YGGCCR	4 cut(s)	630, 747, 982, 1075
AcsI	RAATTY	8 cut(s)	552, 623, 699, 1444, 1547, 1560, 1715, 2236
AcuI	CTGAAG	2 cut(s)	333, 1489
AfiI	CCNNNNNNNGG	8 cut(s)	24, 254, 713, 1787, 1850, 2111, 2130, 2540
AflIII	ACRYGT	2 cut(s)	453, 2217
AgeI	ACCGGT	3 cut(s)	7, 908, 1109
AgsI	TTSAA	9 cut(s)	376, 570, 578, 796, 1241, 1441, 2137, 2480, 2779
AhdI	GACNNNNNGTC	1 cut(s)	330
AhlI	ACTAGT	1 cut(s)	2635
AjnI	CCWGG	4 cut(s)	339, 1849, 2735, 2872
AjuI	GAANNNNNNNTTGG	2 cut(s)	377, 409
AleI	CACNNNNGTG	1 cut(s)	2726
AloI	GAACNNNNNNTCC	2 cut(s)	1463, 1495
Alw21I	GWGCWC	2 cut(s)	1323, 1891
Alw26I	GTCTC	1 cut(s)	1848
AlwI	GGATC	3 cut(s)	724, 1755, 2151
Aor13HI	TCCGGA	1 cut(s)	1207
AoxI	GGCC	8 cut(s)	594, 630, 747, 757, 982, 1075, 1199, 1219
ApeKI	GCWGC	5 cut(s)	730, 1539, 1568, 1827, 2345
ApoI	RAATTY	8 cut(s)	552, 623, 699, 1444, 1547, 1560, 1715, 2236
ArsI	GACNNNNNNTTYG	2 cut(s)	2420, 2452
AsiGI	ACCGGT	3 cut(s)	7, 908, 1109
Asp700I	GAANNNNTTC	4 cut(s)	539, 565, 1190, 1475
Asp718I	GGTACC	2 cut(s)	4, 2513
AspS9I	GGNCC	6 cut(s)	131, 595, 1199, 1219, 1700, 2483
AsuC2I	CCSGG	5 cut(s)	411, 558, 1126, 1396, 1483
AsuHPI	GGTGA	8 cut(s)	164, 295, 898, 923, 1016, 1114, 1568, 2126
AsuII	TTCGAA	1 cut(s)	2439
AvaII	GGWCC	3 cut(s)	131, 1700, 2483
BaeI	ACNNNNGTAYC	2 cut(s)	1079, 1112
BalI	TGGCCA	1 cut(s)	749
BanI	GGYRCC	2 cut(s)	4, 2513
BanII	GRGCYC	3 cut(s)	165, 1323, 1891
BauI	CACGAG	1 cut(s)	216
BbsI	GAAGAC	3 cut(s)	321, 326, 2493
Bbv12I	GWGCWC	2 cut(s)	1323, 1891
BbvI	GCAGC	5 cut(s)	717, 1551, 1555, 1839, 2332
BccI	CCATC	1 cut(s)	2132
BceAI	ACGGC	1 cut(s)	997
BcgI	CGANNNNNNTGC	2 cut(s)	1531, 1565
BciT130I	CCWGG	4 cut(s)	341, 1851, 2737, 2874
BclI	TGATCA	1 cut(s)	1308
BcnI	CCSGG	5 cut(s)	411, 558, 1126, 1396, 1483
BcoDI	GTCTC	1 cut(s)	1848
BcuI	ACTAGT	1 cut(s)	2635
BfaI	CTAG	5 cut(s)	149, 740, 1601, 2636, 2862
BfmI	CTRYAG	1 cut(s)	1825
BglI	GCCNNNNNGGC	2 cut(s)	638, 765
BisI	GCNGC	5 cut(s)	731, 1540, 1569, 1828, 2346
BlsI	GCNGC	5 cut(s)	732, 1541, 1570, 1829, 2347
BmcAI	AGTACT	1 cut(s)	2474
Bme1390I	CCNGG	9 cut(s)	341, 411, 558, 1126, 1396, 1483, 1851, 2737, 2874
Bme18I	GGWCC	3 cut(s)	131, 1700, 2483
BmeRI	GACNNNNNGTC	1 cut(s)	330
BmgT120I	GGNCC	6 cut(s)	131, 595, 1199, 1219, 1700, 2483
BmiI	GGNNCC	6 cut(s)	6, 16, 133, 162, 2515, 2750
BmrFI	CCNGG	9 cut(s)	341, 411, 558, 1126, 1396, 1483, 1851, 2737, 2874
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	1 cut(s)	99
BmuI	ACTGGG	1 cut(s)	166
BpiI	GAAGAC	3 cut(s)	321, 326, 2493
Bpu14I	TTCGAA	1 cut(s)	2439
BpuEI	CTTGAG	7 cut(s)	98, 461, 509, 641, 923, 941, 1312
BpuMI	CCSGG	5 cut(s)	411, 558, 1126, 1396, 1483
BsaI	GGTCTC	1 cut(s)	1848
BsaJI	CCNNGG	1 cut(s)	339
BsaWI	WCCGGW	8 cut(s)	7, 239, 265, 908, 1063, 1109, 1207, 2190
BsaXI	ACNNNNNCTCC	6 cut(s)	167, 197, 1463, 1493, 2842, 2872
Bsc4I	CCNNNNNNNGG	8 cut(s)	24, 254, 713, 1787, 1850, 2111, 2130, 2540
Bse118I	RCCGGY	4 cut(s)	7, 908, 1109, 1221
Bse1I	ACTGG	8 cut(s)	161, 498, 550, 1774, 1888, 2038, 2624, 2760
Bse3DI	GCAATG	3 cut(s)	306, 2526, 2793
BseAI	TCCGGA	1 cut(s)	1207
BseBI	CCWGG	4 cut(s)	341, 1851, 2737, 2874
BseDI	CCNNGG	1 cut(s)	339
BseGI	GGATG	2 cut(s)	2566, 2699
BseLI	CCNNNNNNNGG	8 cut(s)	24, 254, 713, 1787, 1850, 2111, 2130, 2540
BseMI	GCAATG	3 cut(s)	306, 2526, 2793
BseMII	CTCAG	5 cut(s)	439, 699, 1695, 2708, 2755
BseNI	ACTGG	8 cut(s)	161, 498, 550, 1774, 1888, 2038, 2624, 2760
BseRI	GAGGAG	1 cut(s)	26
BseXI	GCAGC	5 cut(s)	717, 1551, 1555, 1839, 2332
BseYI	CCCAGC	1 cut(s)	1935
Bsh1236I	CGCG	2 cut(s)	455, 2051
BshFI	GGCC	8 cut(s)	596, 632, 749, 759, 984, 1077, 1201, 1221
BshNI	GGYRCC	2 cut(s)	4, 2513
BshTI	ACCGGT	3 cut(s)	7, 908, 1109
BsiHKAI	GWGCWC	2 cut(s)	1323, 1891
BsiWI	CGTACG	1 cut(s)	456
BslFI	GGGAC	6 cut(s)	117, 1285, 1582, 2110, 2190, 2289
BslI	CCNNNNNNNGG	8 cut(s)	24, 254, 713, 1787, 1850, 2111, 2130, 2540
BsmAI	GTCTC	1 cut(s)	1848
BsmFI	GGGAC	6 cut(s)	117, 1285, 1582, 2110, 2190, 2289
BsnI	GGCC	8 cut(s)	596, 632, 749, 759, 984, 1077, 1201, 1221
Bso31I	GGTCTC	1 cut(s)	1848
Bsp119I	TTCGAA	1 cut(s)	2439
Bsp1286I	GDGCHC	3 cut(s)	165, 1323, 1891
Bsp13I	TCCGGA	1 cut(s)	1207
Bsp143I	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
BspACI	CCGC	3 cut(s)	531, 2051, 2073
BspANI	GGCC	8 cut(s)	596, 632, 749, 759, 984, 1077, 1201, 1221
BspCNI	CTCAG	5 cut(s)	438, 700, 1694, 2707, 2754
BspEI	TCCGGA	1 cut(s)	1207
BspFNI	CGCG	2 cut(s)	455, 2051
BspLI	GGNNCC	6 cut(s)	6, 16, 133, 162, 2515, 2750
BspMAI	CTGCAG	1 cut(s)	1829
BspPI	GGATC	3 cut(s)	724, 1755, 2151
BspT104I	TTCGAA	1 cut(s)	2439
BspT107I	GGYRCC	2 cut(s)	4, 2513
BspTNI	GGTCTC	1 cut(s)	1848
BsrDI	GCAATG	3 cut(s)	306, 2526, 2793
BsrFI	RCCGGY	4 cut(s)	7, 908, 1109, 1221
BsrI	ACTGG	8 cut(s)	161, 498, 550, 1774, 1888, 2038, 2624, 2760
BssAI	RCCGGY	4 cut(s)	7, 908, 1109, 1221
BssECI	CCNNGG	1 cut(s)	339
BssMI	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
BssSI	CACGAG	1 cut(s)	216
Bst2BI	CACGAG	1 cut(s)	216
Bst2UI	CCWGG	4 cut(s)	341, 1851, 2737, 2874
Bst4CI	ACNGT	7 cut(s)	105, 695, 1996, 2111, 2133, 2543, 2725
Bst6I	CTCTTC	3 cut(s)	172, 1943, 2132
BstBI	TTCGAA	1 cut(s)	2439
BstC8I	GCNNGC	6 cut(s)	615, 634, 751, 871, 1732, 2381
BstDEI	CTNAG	9 cut(s)	425, 503, 583, 708, 1681, 1931, 2694, 2741, 2889
BstEII	GGTNACC	1 cut(s)	1112
BstF5I	GGATG	2 cut(s)	2566, 2699
BstFNI	CGCG	2 cut(s)	455, 2051
BstKTI	GATC	9 cut(s)	348, 495, 719, 1009, 1311, 1750, 1959, 2017, 2146
BstMAI	GTCTC	1 cut(s)	1848
BstMBI	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
BstMWI	GCNNNNNNNGC	6 cut(s)	602, 638, 765, 1900, 2342, 2450
BstNI	CCWGG	4 cut(s)	341, 1851, 2737, 2874
BstNSI	RCATGY	4 cut(s)	2221, 2260, 2500, 2774
BstPI	GGTNACC	1 cut(s)	1112
BstSCI	CCNGG	9 cut(s)	339, 409, 556, 1124, 1394, 1481, 1849, 2735, 2872
BstSFI	CTRYAG	1 cut(s)	1825
BstUI	CGCG	2 cut(s)	455, 2051
BstV1I	GCAGC	5 cut(s)	717, 1551, 1555, 1839, 2332
BstV2I	GAAGAC	3 cut(s)	321, 326, 2493
BstX2I	RGATCY	1 cut(s)	1747
BstXI	CCANNNNNNTGG	3 cut(s)	836, 1196, 1340
BstYI	RGATCY	1 cut(s)	1747
BsuRI	GGCC	8 cut(s)	596, 632, 749, 759, 984, 1077, 1201, 1221
BtgZI	GCGATG	1 cut(s)	1855
BtsCI	GGATG	2 cut(s)	2566, 2699
Cac8I	GCNNGC	6 cut(s)	615, 634, 751, 871, 1732, 2381
Cfr10I	RCCGGY	4 cut(s)	7, 908, 1109, 1221
Cfr13I	GGNCC	6 cut(s)	131, 595, 1199, 1219, 1700, 2483
CseI	GACGC	3 cut(s)	107, 461, 1021
CspAI	ACCGGT	3 cut(s)	7, 908, 1109
DdeI	CTNAG	9 cut(s)	425, 503, 583, 708, 1681, 1931, 2694, 2741, 2889
DpnI	GATC	9 cut(s)	347, 494, 718, 1008, 1310, 1749, 1958, 2016, 2145
DpnII	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
DraI	TTTAAA	2 cut(s)	366, 819
DrdI	GACNNNNNNGTC	1 cut(s)	1862
DriI	GACNNNNNGTC	1 cut(s)	330
DseDI	GACNNNNNNGTC	1 cut(s)	1862
EaeI	YGGCCR	4 cut(s)	630, 747, 982, 1075
Eam1104I	CTCTTC	3 cut(s)	172, 1943, 2132
Eam1105I	GACNNNNNGTC	1 cut(s)	330
EarI	CTCTTC	3 cut(s)	172, 1943, 2132
Ecl136II	GAGCTC	2 cut(s)	1321, 1889
Eco24I	GRGCYC	3 cut(s)	165, 1323, 1891
Eco31I	GGTCTC	1 cut(s)	1848
Eco47I	GGWCC	3 cut(s)	131, 1700, 2483
Eco53kI	GAGCTC	2 cut(s)	1321, 1889
Eco57I	CTGAAG	2 cut(s)	333, 1489
Eco91I	GGTNACC	1 cut(s)	1112
EcoICRI	GAGCTC	2 cut(s)	1321, 1889
EcoO65I	GGTNACC	1 cut(s)	1112
EcoRI	GAATTC	2 cut(s)	699, 1715
EcoRII	CCWGG	4 cut(s)	339, 1849, 2735, 2872
EcoT38I	GRGCYC	3 cut(s)	165, 1323, 1891
FalI	AAGNNNNNCTT	2 cut(s)	1995, 2027
FaqI	GGGAC	6 cut(s)	117, 1285, 1582, 2110, 2190, 2289
FauI	CCCGC	1 cut(s)	524
FbaI	TGATCA	1 cut(s)	1308
FblI	GTMKAC	2 cut(s)	2088, 2841
Fnu4HI	GCNGC	5 cut(s)	731, 1540, 1569, 1828, 2346
FokI	GGATG	2 cut(s)	2573, 2686
FriOI	GRGCYC	3 cut(s)	165, 1323, 1891
Fsp4HI	GCNGC	5 cut(s)	731, 1540, 1569, 1828, 2346
FspBI	CTAG	5 cut(s)	149, 740, 1601, 2636, 2862
GluI	GCNGC	5 cut(s)	731, 1540, 1569, 1828, 2346
GsaI	CCCAGC	1 cut(s)	1939
HaeIII	GGCC	8 cut(s)	596, 632, 749, 759, 984, 1077, 1201, 1221
HgaI	GACGC	3 cut(s)	107, 461, 1021
HincII	GTYRAC	2 cut(s)	298, 2800
HindII	GTYRAC	2 cut(s)	298, 2800
HindIII	AAGCTT	1 cut(s)	580
HphI	GGTGA	8 cut(s)	164, 295, 898, 923, 1016, 1114, 1568, 2126
Hpy166II	GTNNAC	8 cut(s)	92, 298, 805, 1511, 2089, 2731, 2800, 2842
Hpy188I	TCNGA	9 cut(s)	285, 428, 1247, 1579, 1721, 1961, 2388, 2462, 2686
Hpy8I	GTNNAC	8 cut(s)	92, 298, 805, 1511, 2089, 2731, 2800, 2842
Hpy99I	CGWCG	5 cut(s)	455, 674, 695, 781, 1037
HpyAV	CCTTC	5 cut(s)	196, 501, 1781, 2295, 2527
HpyCH4III	ACNGT	7 cut(s)	105, 695, 1996, 2111, 2133, 2543, 2725
HpyCH4IV	ACGT	4 cut(s)	669, 801, 1595, 2031
HpyF10VI	GCNNNNNNNGC	6 cut(s)	602, 638, 765, 1900, 2342, 2450
HpyF3I	CTNAG	9 cut(s)	425, 503, 583, 708, 1681, 1931, 2694, 2741, 2889
HpySE526I	ACGT	4 cut(s)	669, 801, 1595, 2031
Kpn2I	TCCGGA	1 cut(s)	1207
KpnI	GGTACC	2 cut(s)	8, 2517
Ksp22I	TGATCA	1 cut(s)	1308
Kzo9I	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
LmnI	GCTCC	6 cut(s)	160, 188, 1372, 1835, 1886, 2875
Lsp1109I	GCAGC	5 cut(s)	717, 1551, 1555, 1839, 2332
LweI	GCATC	1 cut(s)	99
MaeI	CTAG	5 cut(s)	149, 740, 1601, 2636, 2862
MaeII	ACGT	4 cut(s)	669, 801, 1595, 2031
MaeIII	GTNAC	6 cut(s)	203, 418, 1105, 1112, 2522, 2543
MalI	GATC	9 cut(s)	347, 494, 718, 1008, 1310, 1749, 1958, 2016, 2145
MboI	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
MflI	RGATCY	1 cut(s)	1747
MhlI	GDGCHC	3 cut(s)	165, 1323, 1891
MlsI	TGGCCA	1 cut(s)	749
MluI	ACGCGT	1 cut(s)	453
MluNI	TGGCCA	1 cut(s)	749
MlyI	GAGTC	7 cut(s)	340, 1078, 1447, 1521, 1872, 2423, 2726
MmeI	TCCRAC	3 cut(s)	408, 1411, 1805
Mox20I	TGGCCA	1 cut(s)	749
MroI	TCCGGA	1 cut(s)	1207
MroXI	GAANNNNTTC	4 cut(s)	539, 565, 1190, 1475
MscI	TGGCCA	1 cut(s)	749
MseI	TTAA	5 cut(s)	365, 818, 1520, 1607, 1674
MslI	CAYNNNNRTG	3 cut(s)	834, 1338, 2726
Msp20I	TGGCCA	1 cut(s)	749
MspA1I	CMGCKG	1 cut(s)	753
MspR9I	CCNGG	9 cut(s)	341, 411, 558, 1126, 1396, 1483, 1851, 2737, 2874
MvaI	CCWGG	4 cut(s)	341, 1851, 2737, 2874
MvnI	CGCG	2 cut(s)	455, 2051
MwoI	GCNNNNNNNGC	6 cut(s)	602, 638, 765, 1900, 2342, 2450
NciI	CCSGG	5 cut(s)	411, 558, 1126, 1396, 1483
NdeII	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
NlaIV	GGNNCC	6 cut(s)	6, 16, 133, 162, 2515, 2750
NmuCI	GTSAC	3 cut(s)	203, 418, 2543
NspI	RCATGY	4 cut(s)	2221, 2260, 2500, 2774
NspV	TTCGAA	1 cut(s)	2439
OliI	CACNNNNGTG	1 cut(s)	2726
PciI	ACATGT	1 cut(s)	2217
PcsI	WCGNNNNNNNCGW	2 cut(s)	456, 776
PdmI	GAANNNNTTC	4 cut(s)	539, 565, 1190, 1475
Pfl23II	CGTACG	1 cut(s)	456
PflMI	CCANNNNNTGG	2 cut(s)	1850, 2111
PfoI	TCCNGGA	3 cut(s)	409, 556, 1394
PinAI	ACCGGT	3 cut(s)	7, 908, 1109
PkrI	GCNGC	5 cut(s)	732, 1541, 1570, 1829, 2347
PleI	GAGTC	7 cut(s)	339, 1077, 1447, 1521, 1872, 2423, 2726
PpsI	GAGTC	7 cut(s)	339, 1077, 1447, 1521, 1872, 2423, 2726
PscI	ACATGT	1 cut(s)	2217
Psp124BI	GAGCTC	2 cut(s)	1323, 1891
Psp6I	CCWGG	4 cut(s)	339, 1849, 2735, 2872
PspEI	GGTNACC	1 cut(s)	1112
PspFI	CCCAGC	1 cut(s)	1935
PspGI	CCWGG	4 cut(s)	339, 1849, 2735, 2872
PspLI	CGTACG	1 cut(s)	456
PspN4I	GGNNCC	6 cut(s)	6, 16, 133, 162, 2515, 2750
PspPI	GGNCC	6 cut(s)	131, 595, 1199, 1219, 1700, 2483
PstI	CTGCAG	1 cut(s)	1829
PsuI	RGATCY	1 cut(s)	1747
PvuII	CAGCTG	1 cut(s)	753
RseI	CAYNNNNRTG	3 cut(s)	834, 1338, 2726
SacI	GAGCTC	2 cut(s)	1323, 1891
SaqAI	TTAA	5 cut(s)	365, 818, 1520, 1607, 1674
SatI	GCNGC	5 cut(s)	731, 1540, 1569, 1828, 2346
Sau3AI	GATC	9 cut(s)	345, 492, 716, 1006, 1308, 1747, 1956, 2014, 2143
Sau96I	GGNCC	6 cut(s)	131, 595, 1199, 1219, 1700, 2483
ScaI	AGTACT	1 cut(s)	2474
SchI	GAGTC	7 cut(s)	340, 1078, 1447, 1521, 1872, 2423, 2726
ScrFI	CCNGG	9 cut(s)	341, 411, 558, 1126, 1396, 1483, 1851, 2737, 2874
SduI	GDGCHC	3 cut(s)	165, 1323, 1891
SfaNI	GCATC	1 cut(s)	99
SfcI	CTRYAG	1 cut(s)	1825
SfuI	TTCGAA	1 cut(s)	2439
SgrAI	CRCCGGYG	1 cut(s)	908
SinI	GGWCC	3 cut(s)	131, 1700, 2483
SmiMI	CAYNNNNRTG	3 cut(s)	834, 1338, 2726
SmlI	CTYRAG	7 cut(s)	113, 440, 488, 656, 938, 956, 1291
SmoI	CTYRAG	7 cut(s)	113, 440, 488, 656, 938, 956, 1291
SpeI	ACTAGT	1 cut(s)	2635
SsiI	CCGC	3 cut(s)	531, 2051, 2073
SspI	AATATT	1 cut(s)	590
SspMI	CTAG	5 cut(s)	149, 740, 1601, 2636, 2862
SstI	GAGCTC	2 cut(s)	1323, 1891
StyD4I	CCNGG	9 cut(s)	339, 409, 556, 1124, 1394, 1481, 1849, 2735, 2872
TaaI	ACNGT	7 cut(s)	105, 695, 1996, 2111, 2133, 2543, 2725
TaiI	ACGT	4 cut(s)	672, 804, 1598, 2034
TaqI	TCGA	7 cut(s)	260, 450, 690, 1377, 1530, 2439, 2766
TatI	WGTACW	5 cut(s)	1927, 2005, 2253, 2472, 2716
Tru1I	TTAA	5 cut(s)	365, 818, 1520, 1607, 1674
Tru9I	TTAA	5 cut(s)	365, 818, 1520, 1607, 1674
TseFI	GTSAC	3 cut(s)	203, 418, 2543
TseI	GCWGC	5 cut(s)	730, 1539, 1568, 1827, 2345
Tsp45I	GTSAC	3 cut(s)	203, 418, 2543
TspDTI	ATGAA	9 cut(s)	40, 189, 915, 988, 1098, 1268, 1484, 2567, 2666
TspGWI	ACGGA	4 cut(s)	1341, 1410, 1417, 2313
Van91I	CCANNNNNTGG	2 cut(s)	1850, 2111
VpaK11BI	GGWCC	3 cut(s)	131, 1700, 2483
XapI	RAATTY	8 cut(s)	552, 623, 699, 1444, 1547, 1560, 1715, 2236
XbaI	TCTAGA	1 cut(s)	739
XceI	RCATGY	4 cut(s)	2221, 2260, 2500, 2774
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1847
XmiI	GTMKAC	2 cut(s)	2088, 2841
XmnI	GAANNNNTTC	4 cut(s)	539, 565, 1190, 1475
XspI	CTAG	5 cut(s)	149, 740, 1601, 2636, 2862
ZrmI	AGTACT	1 cut(s)	2474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.