Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

32007443 .. 32016251

Gene Structure 8809 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018568

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2766 bp

ATGATTGGCCCCAAAGGTTCAGGGCTAGAAGACTGGGAGCCCTCTTCTCACTACTATTGCTCCTTCTCTGGAGTTTCATGTGATCACCAATCACGAGTTGTTGCTCTTAACGTGTCCGGTTTGCCTCTGTTTGGCTCGCTTCCGATGGAGATTGGGCTTCTGAACAAGCTCGTCAACCTCAACATTGCCAACAACAACTTAACAGGGAGTCTTCCCCTGGAGATCGCCAACCTGACTTGTTTAAAGCTCTTGAACATCTCCAACAACTTGTTCAGTGGAAGATTTCCCGGAGAAATGACGCTCAGAATGATAGAGCTTGAGGTTCTCGACGCCTATAATAACAACTTCACTGGTCCACTTCCGGTAGAGCTCGTCAATCTCAAAAAGCTCAGACATCTACATCTTGGCGGGAACTACTTCATTGGCGAAATTCCGAGCCTCAACGGTATTGGGCTCACCGGAAAGTTCCCTTCCAGCTTGGCTCTGTTGAAGAATCTCAAGGAATTGTACGTTGGTTACTACAACACTTATAACGGTGGAATTCCACCTGAGTTGGGAACGATGGAGTCGTTGCAAGTGCTTGACATGGCCAGCTGTAACCTCACCGGCACGATTCCGGTCAGTCTAGGCTTTTTGAAGAACTTACGCAAGTTGTTTCTACATATTAACCGTTTGAGTGGCTATATACCTCCACAACTTTCAGGTATGGTGAGTCTGACGTCTTTGGATCTCTCCATTAACGAACTCACCGGAGAAATGCCAGAAAGTCTCTCGGAGCTCAAGAACCTTACCCTACTCAACCTGTATACGAACAATTTTTACGGCCCGATACCCAACTTCATTGGCGATCTTCCTCATCTGGAGGTACTTCAAGTTTGGGAGAATAGCTTCATATTTGAACTACCTGAGAGTCTTGGCCGGAATGGAAGGCTCAAGGACCTCGATGTAACCGGTAACCACCTCACCGGGTTGATTCCTCGAGATTTGTGCAAAGGAGGGAGGTTGAAGACGTTGATTCTGATGGATAATCACTTCTTTGGGCCGATTCCGGAGGCATTGGGTCGGTGCAAGACGCTAGTCAAAATCAGATTGGGGAAAAACTCACTCAACGGGACTATTCCTGCCGGAATCTTCAGCTTACCTGATGTGAACATGATTGAGCTCAATGATAATTACTTGTCCGGAGAACTACCGATGCAGCTGTCTAGTGCCTCACTCGGAATCCTCACTCTTTCCACTAATCAAATTTCTGGAAGAATCCCATCTACCATTGGAAATCTACGGAATTTACAAACTCTTTTGCTAGATATGAACAGATTCTCTGGGCCAGTACCAGAGGAAATCTTCAGTTTACAATTGCTGACTAAACTCAACATAAGCACCAACAATATTTGTGGTGAAATTCCGGCTTCAATCTCTAGTTGTTCCTGTCTAACGTTCCTAGATATTAGTCAAAACACTTTTGTTGGGGAAGTCCCAAGAGGGATAGGAAAACTGAAAGTAATTCAGTTTCTTAATTTCTCAAGGAACAAGCTAACAGGTGCAATACCTGTAGAATTCATATACATGGGGAGCCTCACAAATTTGGATCTCTCCTATAACAATTTCATTGGTAAGATCCCAATTGAATGTCAGTTTTTATGGTCGAATGCTTCGTTTGAAGGAAATCCCAATCTGTGTTCGGCGGAAAAGGTGTTGTGCAGCTCTGATAACCACCCACGACCTCGAGACCATAAAGTCATCATCGTTGGTCTCACTGGAGCTCTGCTAACTTTGCTACTCATGTTGCTTCTTATATATGTAGTTATCAGGGTTTATAGGAAGAGGAAGACCACAAAGGTAGAGAAATGTGGCTCTTGGAAGCTAACAGTTTTCCAAGTACAGCTTGATCTCAACGTAGAGAACGTTCTCCATTATCTAAACGCAGAGAACATTATCGGCAAGGGTGGTGCTGGAGTTGTCTTCCGGGGGACAATGCCAAATGGTGGTGATGTTGCAATCAAACAGCTGCAGAGGGATCAGGGATTTTCGGCTGAAATTAAAACATTAGGACAAATCAAGCACCGGAATATAGTGCGGTTGTTGGGGTACATGTCGAACAAGGACACAAATTTGTTGCTGTACGAGTACATGCCTAATGGTAGCTTGGGACAACTGTTGCATGGGCCTAATGGAGGGCATTTGGAGTGGGAAATGAGGTACAAGATTGCGGTGGAAGCTGCCAAGGGATTGTGTTATCTCCATCACGACTGTTCGCCTCTGATTATACACAGGGATGTAAAGTCCCATAACATCTTGCTGGACTCTAACTTTGAAGCTCATGTTGCTGATTTTGGGCTTGCCAAGTACTTTCAAGGACCGGCAGACTGCATGTCTTCGCTTGCTGGTACCTTTGGTTACATTGCCCCAAAGTACGGTTGCACACTGAAAGTGGATGAGAAAATCGATGTGTACAGCTTTGGTGTGGTTCTGCTGGAACTAGTAACAGGGAGGAAACCAGTGGTGGAACTAGTCGAAGAAGCTGTGAACCTGGTAAGTTGGGTAAGGAAAACCACATCAGAAGTCCCTCAGTCATCCACTGCATCACCTGTACTTGCAGTTGTAGACTCCAGGCTCAGTGGGTTCCCATTGGCAGGTGTCGAGTATGTGTTCAAGATAGCAATGATGTGCGTTGAGAATCACAGTTCTGCAAGGCCTACGATGATGGAAGTCGTCTACTTGCTCACTAATCCTCCTCCTCCTGCTCCAGGCATGACAAACCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

922

Amino Acids

101.21

Weight (kDa)

6.85

Isoelectric Point (pI)

32.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	45 - 136	2.4e-06	Leucine-rich repeat region
LRR_8	PF13855	190 - 249	5.1e-06	Leucine rich repeat
LRR_14	PF23598	444 - 513	1.6e-06	Leucine-rich repeat region
Pkinase	PF00069	642 - 905	1.9e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	642 - 905	1.7e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	531
AarI	CACCTGC	1 cut(s)	2624
AasI	GACNNNNNNGTC	1 cut(s)	1736
AatII	GACGTC	1 cut(s)	722
Acc36I	ACCTGC	1 cut(s)	2624
Acc65I	GGTACC	1 cut(s)	2387
AccB1I	GGYRCC	1 cut(s)	2387
AccB7I	CCANNNNNTGG	1 cut(s)	1985
AccI	GTMKAC	3 cut(s)	806, 2604, 2715
AccIII	TCCGGA	2 cut(s)	1048, 1181
AciI	CCGC	4 cut(s)	408, 1685, 2077, 2210
AclI	AACGTT	2 cut(s)	1436, 1905
AclWI	GGATC	4 cut(s)	735, 1596, 1612, 2025
AcoI	YGGCCR	2 cut(s)	588, 916
AcsI	RAATTY	8 cut(s)	429, 540, 1245, 1285, 1401, 1556, 1582, 2110
AcuI	CTGAAG	2 cut(s)	1117, 1330
AcyI	GRCGYC	2 cut(s)	330, 719
AfiI	CCNNNNNNNGG	7 cut(s)	131, 554, 1985, 2174, 2414, 2633, 2747
AflIII	ACRYGT	2 cut(s)	111, 2091
AgeI	ACCGGT	1 cut(s)	950
AhdI	GACNNNNNGTC	1 cut(s)	2371
AhlI	ACTAGT	2 cut(s)	2479, 2509
AjnI	CCWGG	4 cut(s)	216, 2529, 2609, 2746
AjuI	GAANNNNNNNTTGG	4 cut(s)	254, 286, 495, 527
Alw21I	GWGCWC	4 cut(s)	372, 780, 1164, 1765
Alw26I	GTCTC	3 cut(s)	773, 1722, 1757
AlwI	GGATC	4 cut(s)	735, 1596, 1612, 2025
Ama87I	CYCGRG	2 cut(s)	978, 1725
Aor13HI	TCCGGA	2 cut(s)	1048, 1181
AoxI	GGCC	8 cut(s)	7, 588, 823, 916, 1040, 1325, 2165, 2693
ApeKI	GCWGC	4 cut(s)	1198, 1701, 2008, 2219
ApoI	RAATTY	8 cut(s)	429, 540, 1245, 1285, 1401, 1556, 1582, 2110
ArsI	GACNNNNNNTTYG	2 cut(s)	2294, 2326
AsiGI	ACCGGT	1 cut(s)	950
Asp700I	GAANNNNTTC	3 cut(s)	416, 887, 1316
Asp718I	GGTACC	1 cut(s)	2387
AspS9I	GGNCC	8 cut(s)	8, 353, 824, 937, 1040, 1325, 2165, 2357
AsuC2I	CCSGG	3 cut(s)	288, 967, 1967
AsuHPI	GGTGA	9 cut(s)	77, 448, 595, 721, 739, 955, 1409, 2000, 2577
AvaI	CYCGRG	2 cut(s)	978, 1725
AvaII	GGWCC	3 cut(s)	353, 937, 2357
BalI	TGGCCA	1 cut(s)	590
BanI	GGYRCC	1 cut(s)	2387
BanII	GRGCYC	6 cut(s)	42, 372, 456, 780, 1164, 1765
BauI	CACGAG	1 cut(s)	93
BbsI	GAAGAC	6 cut(s)	36, 203, 1013, 1835, 1954, 2367
Bbv12I	GWGCWC	4 cut(s)	372, 780, 1164, 1765
BbvI	GCAGC	4 cut(s)	1210, 1713, 1995, 2206
BccI	CCATC	6 cut(s)	139, 556, 1015, 1270, 2250, 2698
BceAI	ACGGC	1 cut(s)	838
BcgI	CGANNNNNNTGC	2 cut(s)	969, 1003
BciT130I	CCWGG	4 cut(s)	218, 2531, 2611, 2748
BclI	TGATCA	1 cut(s)	82
BcnI	CCSGG	3 cut(s)	288, 967, 1967
BcoDI	GTCTC	3 cut(s)	773, 1722, 1757
BcuI	ACTAGT	2 cut(s)	2479, 2509
BfaI	CTAG	9 cut(s)	26, 626, 1076, 1206, 1304, 1419, 1442, 2480, 2510
BfmI	CTRYAG	2 cut(s)	1551, 2009
BfuAI	ACCTGC	1 cut(s)	2624
BisI	GCNGC	4 cut(s)	1199, 1702, 2009, 2220
BlsI	GCNGC	4 cut(s)	1200, 1703, 2010, 2221
BmcAI	AGTACT	1 cut(s)	2348
Bme1390I	CCNGG	7 cut(s)	218, 288, 967, 1967, 2531, 2611, 2748
Bme18I	GGWCC	3 cut(s)	353, 937, 2357
BmeRI	GACNNNNNGTC	1 cut(s)	2371
BmeT110I	CYCGRG	2 cut(s)	978, 1725
BmgT120I	GGNCC	8 cut(s)	8, 353, 824, 937, 1040, 1325, 2165, 2357
BmiI	GGNNCC	5 cut(s)	10, 39, 1574, 2389, 2624
BmrFI	CCNGG	7 cut(s)	218, 288, 967, 1967, 2531, 2611, 2748
BmrI	ACTGGG	1 cut(s)	43
BmsI	GCATC	2 cut(s)	1185, 2591
BmuI	ACTGGG	1 cut(s)	43
BoxI	GACNNNNGTC	1 cut(s)	1076
BpiI	GAAGAC	6 cut(s)	36, 203, 1013, 1835, 1954, 2367
BpmI	CTGGAG	7 cut(s)	90, 239, 881, 1779, 1974, 2593, 2730
BpuEI	CTTGAG	5 cut(s)	338, 482, 764, 917, 1507
BpuMI	CCSGG	3 cut(s)	288, 967, 1967
Bsa29I	ATCGAT	1 cut(s)	2445
BsaHI	GRCGYC	2 cut(s)	330, 719
BsaI	GGTCTC	2 cut(s)	1722, 1757
BsaJI	CCNNGG	3 cut(s)	216, 1966, 2223
BsaWI	WCCGGW	9 cut(s)	116, 361, 458, 616, 749, 950, 1048, 1181, 2064
BsaXI	ACNNNNNCTCC	4 cut(s)	44, 74, 2716, 2746
Bsc4I	CCNNNNNNNGG	7 cut(s)	131, 554, 1985, 2174, 2414, 2633, 2747
Bse118I	RCCGGY	3 cut(s)	605, 950, 2359
Bse1I	ACTGG	5 cut(s)	38, 355, 1328, 1762, 2498
Bse3DI	GCAATG	3 cut(s)	183, 2400, 2667
BseAI	TCCGGA	2 cut(s)	1048, 1181
BseBI	CCWGG	4 cut(s)	218, 2531, 2611, 2748
BseCI	ATCGAT	1 cut(s)	2445
BseDI	CCNNGG	3 cut(s)	216, 1966, 2223
BseGI	GGATG	3 cut(s)	2281, 2440, 2573
BseLI	CCNNNNNNNGG	7 cut(s)	131, 554, 1985, 2174, 2414, 2633, 2747
BseMI	GCAATG	3 cut(s)	183, 2400, 2667
BseMII	CTCAG	6 cut(s)	316, 403, 540, 897, 2582, 2629
BseNI	ACTGG	5 cut(s)	38, 355, 1328, 1762, 2498
BseRI	GAGGAG	2 cut(s)	2724, 2727
BseXI	GCAGC	4 cut(s)	1210, 1713, 1995, 2206
BsgI	GTGCAG	1 cut(s)	1720
BshFI	GGCC	8 cut(s)	9, 590, 825, 918, 1042, 1327, 2167, 2695
BshNI	GGYRCC	1 cut(s)	2387
BshTI	ACCGGT	1 cut(s)	950
BshVI	ATCGAT	1 cut(s)	2445
BsiHKAI	GWGCWC	4 cut(s)	372, 780, 1164, 1765
BsiHKCI	CYCGRG	2 cut(s)	978, 1725
BslFI	GGGAC	6 cut(s)	1126, 1460, 1984, 2163, 2269, 2549
BslI	CCNNNNNNNGG	7 cut(s)	131, 554, 1985, 2174, 2414, 2633, 2747
BsmAI	GTCTC	3 cut(s)	773, 1722, 1757
BsmFI	GGGAC	6 cut(s)	1126, 1460, 1984, 2163, 2269, 2549
BsmI	GAATGC	1 cut(s)	1654
BsnI	GGCC	8 cut(s)	9, 590, 825, 918, 1042, 1327, 2167, 2695
Bso31I	GGTCTC	2 cut(s)	1722, 1757
BsoBI	CYCGRG	2 cut(s)	978, 1725
Bsp1286I	GDGCHC	6 cut(s)	42, 372, 456, 780, 1164, 1765
Bsp13I	TCCGGA	2 cut(s)	1048, 1181
Bsp1407I	TGTACA	1 cut(s)	2451
Bsp143I	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
BspACI	CCGC	4 cut(s)	408, 1685, 2077, 2210
BspANI	GGCC	8 cut(s)	9, 590, 825, 918, 1042, 1327, 2167, 2695
BspCNI	CTCAG	6 cut(s)	315, 402, 541, 898, 2581, 2628
BspDI	ATCGAT	1 cut(s)	2445
BspEI	TCCGGA	2 cut(s)	1048, 1181
BspLI	GGNNCC	5 cut(s)	10, 39, 1574, 2389, 2624
BspMAI	CTGCAG	1 cut(s)	2013
BspMI	ACCTGC	1 cut(s)	2624
BspPI	GGATC	4 cut(s)	735, 1596, 1612, 2025
BspT107I	GGYRCC	1 cut(s)	2387
BspTNI	GGTCTC	2 cut(s)	1722, 1757
BsrDI	GCAATG	3 cut(s)	183, 2400, 2667
BsrFI	RCCGGY	3 cut(s)	605, 950, 2359
BsrGI	TGTACA	1 cut(s)	2451
BsrI	ACTGG	5 cut(s)	38, 355, 1328, 1762, 2498
BssAI	RCCGGY	3 cut(s)	605, 950, 2359
BssECI	CCNNGG	3 cut(s)	216, 1966, 2223
BssMI	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
BssNAI	GTATAC	1 cut(s)	807
BssNI	GRCGYC	2 cut(s)	330, 719
BssSI	CACGAG	1 cut(s)	93
BssT1I	CCWWGG	1 cut(s)	2223
Bst1107I	GTATAC	1 cut(s)	807
Bst2BI	CACGAG	1 cut(s)	93
Bst2UI	CCWGG	4 cut(s)	218, 2531, 2611, 2748
Bst4CI	ACNGT	8 cut(s)	446, 536, 671, 1870, 2157, 2252, 2417, 2684
Bst6I	CTCTTC	2 cut(s)	49, 1817
BstACI	GRCGYC	2 cut(s)	330, 719
BstAUI	TGTACA	1 cut(s)	2451
BstC8I	GCNNGC	4 cut(s)	137, 592, 2340, 2382
BstDEI	CTNAG	7 cut(s)	302, 389, 549, 906, 2568, 2615, 2763
BstEII	GGTNACC	1 cut(s)	953
BstENI	CCTNNNNNAGG	2 cut(s)	2172, 2745
BstF5I	GGATG	3 cut(s)	2281, 2440, 2573
BstKTI	GATC	8 cut(s)	85, 225, 730, 850, 1591, 1620, 1891, 2020
BstMAI	GTCTC	3 cut(s)	773, 1722, 1757
BstMBI	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
BstMWI	GCNNNNNNNGC	3 cut(s)	1774, 2216, 2324
BstNI	CCWGG	4 cut(s)	218, 2531, 2611, 2748
BstNSI	RCATGY	3 cut(s)	2095, 2134, 2374
BstPAI	GACNNNNGTC	1 cut(s)	1076
BstPI	GGTNACC	1 cut(s)	953
BstSCI	CCNGG	7 cut(s)	216, 286, 965, 1965, 2529, 2609, 2746
BstSFI	CTRYAG	2 cut(s)	1551, 2009
BstV1I	GCAGC	4 cut(s)	1210, 1713, 1995, 2206
BstV2I	GAAGAC	6 cut(s)	36, 203, 1013, 1835, 1954, 2367
BstX2I	RGATCY	3 cut(s)	727, 1588, 1617
BstYI	RGATCY	3 cut(s)	727, 1588, 1617
BstZ17I	GTATAC	1 cut(s)	807
Bsu15I	ATCGAT	1 cut(s)	2445
BsuRI	GGCC	8 cut(s)	9, 590, 825, 918, 1042, 1327, 2167, 2695
BsuTUI	ATCGAT	1 cut(s)	2445
BtsCI	GGATG	3 cut(s)	2281, 2440, 2573
BtsI	GCAGTG	1 cut(s)	2577
BtsIMutI	CAGTG	7 cut(s)	280, 348, 1755, 2423, 2505, 2577, 2623
BveI	ACCTGC	1 cut(s)	2624
Cac8I	GCNNGC	4 cut(s)	137, 592, 2340, 2382
Cfr10I	RCCGGY	3 cut(s)	605, 950, 2359
Cfr13I	GGNCC	8 cut(s)	8, 353, 824, 937, 1040, 1325, 2165, 2357
ClaI	ATCGAT	1 cut(s)	2445
CseI	GACGC	3 cut(s)	307, 338, 1081
CsiI	ACCWGGT	1 cut(s)	2529
CspAI	ACCGGT	1 cut(s)	950
CspCI	CAANNNNNGTGG	4 cut(s)	534, 569, 1375, 1410
DdeI	CTNAG	7 cut(s)	302, 389, 549, 906, 2568, 2615, 2763
DpnI	GATC	8 cut(s)	84, 224, 729, 849, 1590, 1619, 1890, 2019
DpnII	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
DraI	TTTAAA	1 cut(s)	243
DrdI	GACNNNNNNGTC	1 cut(s)	1736
DriI	GACNNNNNGTC	1 cut(s)	2371
DseDI	GACNNNNNNGTC	1 cut(s)	1736
EaeI	YGGCCR	2 cut(s)	588, 916
Eam1104I	CTCTTC	2 cut(s)	49, 1817
Eam1105I	GACNNNNNGTC	1 cut(s)	2371
EarI	CTCTTC	2 cut(s)	49, 1817
EciI	GGCGGA	1 cut(s)	1700
Ecl136II	GAGCTC	4 cut(s)	370, 778, 1162, 1763
Eco130I	CCWWGG	1 cut(s)	2223
Eco147I	AGGCCT	1 cut(s)	2695
Eco24I	GRGCYC	6 cut(s)	42, 372, 456, 780, 1164, 1765
Eco31I	GGTCTC	2 cut(s)	1722, 1757
Eco47I	GGWCC	3 cut(s)	353, 937, 2357
Eco53kI	GAGCTC	4 cut(s)	370, 778, 1162, 1763
Eco57I	CTGAAG	2 cut(s)	1117, 1330
Eco88I	CYCGRG	2 cut(s)	978, 1725
Eco91I	GGTNACC	1 cut(s)	953
EcoICRI	GAGCTC	4 cut(s)	370, 778, 1162, 1763
EcoNI	CCTNNNNNAGG	2 cut(s)	2172, 2745
EcoO109I	RGGNCCY	1 cut(s)	937
EcoO65I	GGTNACC	1 cut(s)	953
EcoRI	GAATTC	2 cut(s)	540, 1556
EcoRII	CCWGG	4 cut(s)	216, 2529, 2609, 2746
EcoT14I	CCWWGG	1 cut(s)	2223
EcoT38I	GRGCYC	6 cut(s)	42, 372, 456, 780, 1164, 1765
ErhI	CCWWGG	1 cut(s)	2223
FalI	AAGNNNNNCTT	2 cut(s)	1869, 1901
FaqI	GGGAC	6 cut(s)	1126, 1460, 1984, 2163, 2269, 2549
FauI	CCCGC	1 cut(s)	401
FbaI	TGATCA	1 cut(s)	82
FblI	GTMKAC	3 cut(s)	806, 2604, 2715
Fnu4HI	GCNGC	4 cut(s)	1199, 1702, 2009, 2220
FokI	GGATG	3 cut(s)	2288, 2447, 2560
FriOI	GRGCYC	6 cut(s)	42, 372, 456, 780, 1164, 1765
Fsp4HI	GCNGC	4 cut(s)	1199, 1702, 2009, 2220
FspBI	CTAG	9 cut(s)	26, 626, 1076, 1206, 1304, 1419, 1442, 2480, 2510
GluI	GCNGC	4 cut(s)	1199, 1702, 2009, 2220
GsuI	CTGGAG	7 cut(s)	90, 239, 881, 1779, 1974, 2593, 2730
HaeIII	GGCC	8 cut(s)	9, 590, 825, 918, 1042, 1327, 2167, 2695
HgaI	GACGC	3 cut(s)	307, 338, 1081
Hin1I	GRCGYC	2 cut(s)	330, 719
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HphI	GGTGA	9 cut(s)	77, 448, 595, 721, 739, 955, 1409, 2000, 2577
Hpy166II	GTNNAC	9 cut(s)	175, 356, 807, 1150, 1352, 2452, 2527, 2605, 2716
Hpy8I	GTNNAC	9 cut(s)	175, 356, 807, 1150, 1352, 2452, 2527, 2605, 2716
Hpy99I	CGWCG	1 cut(s)	332
HpyAV	CCTTC	4 cut(s)	73, 480, 921, 1655
HpyCH4III	ACNGT	8 cut(s)	446, 536, 671, 1870, 2157, 2252, 2417, 2684
HpyCH4IV	ACGT	7 cut(s)	111, 510, 719, 1010, 1436, 1896, 1905
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1774, 2216, 2324
HpyF3I	CTNAG	7 cut(s)	302, 389, 549, 906, 2568, 2615, 2763
HpySE526I	ACGT	7 cut(s)	111, 510, 719, 1010, 1436, 1896, 1905
Hsp92I	GRCGYC	2 cut(s)	330, 719
Kpn2I	TCCGGA	2 cut(s)	1048, 1181
KpnI	GGTACC	1 cut(s)	2391
Ksp22I	TGATCA	1 cut(s)	82
Kzo9I	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
LmnI	GCTCC	6 cut(s)	37, 65, 775, 1572, 1760, 2749
Lsp1109I	GCAGC	4 cut(s)	1210, 1713, 1995, 2206
LweI	GCATC	2 cut(s)	1185, 2591
MabI	ACCWGGT	1 cut(s)	2529
MaeI	CTAG	9 cut(s)	26, 626, 1076, 1206, 1304, 1419, 1442, 2480, 2510
MaeII	ACGT	7 cut(s)	111, 510, 719, 1010, 1436, 1896, 1905
MaeIII	GTNAC	6 cut(s)	515, 596, 946, 953, 2396, 2482
MalI	GATC	8 cut(s)	84, 224, 729, 849, 1590, 1619, 1890, 2019
MboI	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
MfeI	CAATTG	2 cut(s)	1355, 1623
MflI	RGATCY	3 cut(s)	727, 1588, 1617
MhlI	GDGCHC	6 cut(s)	42, 372, 456, 780, 1164, 1765
MlsI	TGGCCA	1 cut(s)	590
MluNI	TGGCCA	1 cut(s)	590
MlyI	GAGTC	6 cut(s)	217, 575, 721, 919, 2297, 2600
MmeI	TCCRAC	1 cut(s)	285
Mox20I	TGGCCA	1 cut(s)	590
MroI	TCCGGA	2 cut(s)	1048, 1181
MroXI	GAANNNNTTC	3 cut(s)	416, 887, 1316
MscI	TGGCCA	1 cut(s)	590
MseI	TTAA	7 cut(s)	108, 200, 242, 666, 738, 1515, 2040
MslI	CAYNNNNRTG	2 cut(s)	1565, 2274
Msp20I	TGGCCA	1 cut(s)	590
MspA1I	CMGCKG	3 cut(s)	594, 1201, 2008
MspR9I	CCNGG	7 cut(s)	218, 288, 967, 1967, 2531, 2611, 2748
MunI	CAATTG	2 cut(s)	1355, 1623
Mva1269I	GAATGC	1 cut(s)	1654
MvaI	CCWGG	4 cut(s)	218, 2531, 2611, 2748
MwoI	GCNNNNNNNGC	3 cut(s)	1774, 2216, 2324
NciI	CCSGG	3 cut(s)	288, 967, 1967
NdeII	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
NlaIV	GGNNCC	5 cut(s)	10, 39, 1574, 2389, 2624
NspI	RCATGY	3 cut(s)	2095, 2134, 2374
PaeR7I	CTCGAG	2 cut(s)	978, 1725
PaqCI	CACCTGC	1 cut(s)	2624
PceI	AGGCCT	1 cut(s)	2695
PciI	ACATGT	1 cut(s)	2091
PcsI	WCGNNNNNNNCGW	3 cut(s)	566, 1652, 1902
PctI	GAATGC	1 cut(s)	1654
PdmI	GAANNNNTTC	3 cut(s)	416, 887, 1316
PflMI	CCANNNNNTGG	1 cut(s)	1985
PfoI	TCCNGGA	1 cut(s)	286
PinAI	ACCGGT	1 cut(s)	950
PkrI	GCNGC	4 cut(s)	1200, 1703, 2010, 2221
PleI	GAGTC	6 cut(s)	216, 574, 720, 918, 2297, 2600
PpsI	GAGTC	6 cut(s)	216, 574, 720, 918, 2297, 2600
PpuMI	RGGWCCY	1 cut(s)	937
PscI	ACATGT	1 cut(s)	2091
PshAI	GACNNNNGTC	1 cut(s)	1076
PsiI	TTATAA	1 cut(s)	531
Psp124BI	GAGCTC	4 cut(s)	372, 780, 1164, 1765
Psp1406I	AACGTT	2 cut(s)	1436, 1905
Psp5II	RGGWCCY	1 cut(s)	937
Psp6I	CCWGG	4 cut(s)	216, 2529, 2609, 2746
PspEI	GGTNACC	1 cut(s)	953
PspGI	CCWGG	4 cut(s)	216, 2529, 2609, 2746
PspN4I	GGNNCC	5 cut(s)	10, 39, 1574, 2389, 2624
PspPI	GGNCC	8 cut(s)	8, 353, 824, 937, 1040, 1325, 2165, 2357
PspPPI	RGGWCCY	1 cut(s)	937
PsrI	GAACNNNNNNTAC	4 cut(s)	803, 835, 1890, 1922
PstI	CTGCAG	1 cut(s)	2013
PsuI	RGATCY	3 cut(s)	727, 1588, 1617
PvuII	CAGCTG	3 cut(s)	594, 1201, 2008
RseI	CAYNNNNRTG	2 cut(s)	1565, 2274
SacI	GAGCTC	4 cut(s)	372, 780, 1164, 1765
SaqAI	TTAA	7 cut(s)	108, 200, 242, 666, 738, 1515, 2040
SatI	GCNGC	4 cut(s)	1199, 1702, 2009, 2220
Sau3AI	GATC	8 cut(s)	82, 222, 727, 847, 1588, 1617, 1888, 2017
Sau96I	GGNCC	8 cut(s)	8, 353, 824, 937, 1040, 1325, 2165, 2357
ScaI	AGTACT	1 cut(s)	2348
SchI	GAGTC	6 cut(s)	217, 575, 721, 919, 2297, 2600
ScrFI	CCNGG	7 cut(s)	218, 288, 967, 1967, 2531, 2611, 2748
SduI	GDGCHC	6 cut(s)	42, 372, 456, 780, 1164, 1765
SexAI	ACCWGGT	1 cut(s)	2529
SfaNI	GCATC	2 cut(s)	1185, 2591
SfcI	CTRYAG	2 cut(s)	1551, 2009
Sfr274I	CTCGAG	2 cut(s)	978, 1725
SinI	GGWCC	3 cut(s)	353, 937, 2357
SlaI	CTCGAG	2 cut(s)	978, 1725
SmiMI	CAYNNNNRTG	2 cut(s)	1565, 2274
SmlI	CTYRAG	7 cut(s)	317, 497, 779, 932, 978, 1522, 1725
SmoI	CTYRAG	7 cut(s)	317, 497, 779, 932, 978, 1522, 1725
SpeI	ACTAGT	2 cut(s)	2479, 2509
SseBI	AGGCCT	1 cut(s)	2695
SsiI	CCGC	4 cut(s)	408, 1685, 2077, 2210
SspI	AATATT	1 cut(s)	1390
SspMI	CTAG	9 cut(s)	26, 626, 1076, 1206, 1304, 1419, 1442, 2480, 2510
SstI	GAGCTC	4 cut(s)	372, 780, 1164, 1765
StuI	AGGCCT	1 cut(s)	2695
StyD4I	CCNGG	7 cut(s)	216, 286, 965, 1965, 2529, 2609, 2746
StyI	CCWWGG	1 cut(s)	2223
TaaI	ACNGT	8 cut(s)	446, 536, 671, 1870, 2157, 2252, 2417, 2684
TaiI	ACGT	7 cut(s)	114, 513, 722, 1013, 1439, 1899, 1908
TaqI	TCGA	9 cut(s)	327, 942, 979, 1646, 1726, 2096, 2445, 2514, 2640
TatI	WGTACW	5 cut(s)	1879, 2127, 2346, 2451, 2590
Tru1I	TTAA	7 cut(s)	108, 200, 242, 666, 738, 1515, 2040
Tru9I	TTAA	7 cut(s)	108, 200, 242, 666, 738, 1515, 2040
TscAI	CASTG	7 cut(s)	280, 355, 1762, 2430, 2505, 2584, 2623
TseI	GCWGC	4 cut(s)	1198, 1701, 2008, 2219
TspDTI	ATGAA	7 cut(s)	66, 409, 829, 880, 1325, 1549, 1597
TspGWI	ACGGA	1 cut(s)	1297
TspRI	CASTG	7 cut(s)	280, 355, 1762, 2430, 2505, 2584, 2623
Van91I	CCANNNNNTGG	1 cut(s)	1985
VpaK11BI	GGWCC	3 cut(s)	353, 937, 2357
XagI	CCTNNNNNAGG	2 cut(s)	2172, 2745
XapI	RAATTY	8 cut(s)	429, 540, 1245, 1285, 1401, 1556, 1582, 2110
XceI	RCATGY	3 cut(s)	2095, 2134, 2374
XhoI	CTCGAG	2 cut(s)	978, 1725
XmiI	GTMKAC	3 cut(s)	806, 2604, 2715
XmnI	GAANNNNTTC	3 cut(s)	416, 887, 1316
XspI	CTAG	9 cut(s)	26, 626, 1076, 1206, 1304, 1419, 1442, 2480, 2510
ZraI	GACGTC	1 cut(s)	720
ZrmI	AGTACT	1 cut(s)	2348

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.