Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Reverse (-)

26972229 .. 26976699

Gene Structure 4471 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1139200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2964 bp

ATGGAAAATAAAAAATTTAAGAAGCTAATATCCGCCTACATTTCCTTCATCATATTCCTTTTGTTCCCATCATCGCCGTGCGCCGACGACCTCGACGCGCTACTGAAGCTCAAGAAGGCCATGAATATTACCGGGTGGGAGCCCTCCGCCCAGCACTATTGCTCCTTTTCTGGAGTTTCATGCGACCAGCGATCACGAGTGGTTGCTCTTAACGTCTCAAATATGCCGCCCGTGATGCATCGAGGCTCGATTCCAGCAGAGATCGGGCTCCTAAACAAGCTCGTGAACCTCAACCTCGCCGAAAACAACCTCTCGGGGAGGCTTCCCACGGAGATGGCCAACCTGACGGCGTTAACGCATTTGAACATCTCAAACAACTTCTTCAGCGGCACGTTCCCCGGAGAAATCACTCTCGGGATGACGGAGCTTGAGGTCCTTGACGCCTACAATAACAACTTCACGGGACAGCTGCCGCTGGAGCTCGCCGCCGGCTGTACAAAGCTCAAACATCTTCATCTGGGAGGCAACTACTTCACGGGAAACATTCCCGAAAGTTATTCTAATATCCGGAGCTTGGAGTATTTAGGCCTCAACGGCAATTTTCTCACCGGGAAGCTCCCGGCAAGCTTGGCACGGTTAAAGAACCTCAAGGAATTGTACCTAGGGAACGATAACAGTTACGAAGGCGGGATTCCACCGGAGTTTGGATCGCTGCCGTTGCTGAAAGTGCTCTACATGGCCAGCTGTAACCTCACTGGCAAGATTCCCACGAGTCTGAGCCTTTTGAAGAATTTGCACTCGTTGTGTTTACAAGTAAATCGGCTCGGTGGTTTTATACCACCGGAACTTTCGAGCATGGCGAGCCTCGTGTCCTTGGATCTCGCCGAAAACGAACTCACCGGAGAAATACCCGAACGGTTCTCAGAGCTGAAGAGCATTAGGTCCATCTATCTGTATTGGAACAATCTTTACGGTCCCATACCCAAGTTCATCGGTGACCTTCCTCATCTGGAGATTCTTCATCTGTGGGGGAACAACTTTACATTCCAACTACCCGAAAGTCTTGGCCAGAACGGCAGGCTTACGGACTTGGATGTCTCCGGAAACAAACTAACCGGGTTGATTCCTCGGAATTTGTGCTTAGGCGGGAGGCTGAGGACGCTGATTCTGATGGATAACAACTTCTTTGGGCCCATTCCGTTGGAGCTCGGCCGATGCAAGTCTCTTGTCAAAATCCGAATGAAGAAAAACAGAATCAGCGGGACTATTCCTGTTGGGATCCTCAACTTGCCGAGATTGGGCATGATTGAGATGAATGATAACTGCCTGTCCGGTCAACTTCCAACACAAATGACAGCGGGCACACTCGCAATCCTCTCGCTTTCTGGGAATCAGATTTCGGGGAGGATCCCACCCGCAATTGGAAACCTCAACAACTTAACAACTCTCTCGCTGGAGATGAACCGATTTTCTGGGAAAATACCAGACGAAATCTTCAACTTGATAGTGTTATCAAAAATCAACATCAGCGTCAACAAGCTCGGTGGCGAAATTCCAGCTTCTGTTTCTAATTGTTCGTCTCTAGGGTCGCTTGATTTGAGTCGAAACAATTTGGTCGGCGAAATCCCAAGAGGGGTAGAGAAGCTGAAACTAGTTTATCTTCTGAATATGTCTCGGAACCGACTCACTGGCCAAATACCTGACGGAATACCCGGCATGGAAACCCTTACAACTTTGGATCTCTCTTATAACAATTTCACCGGTAGAATCCCAACCAGTGATCATTTTCTGGATATCGCGTCGTTTCGTGGGAACCCCTATCTCTGTACCAACGTCTCACGCCCGTGTTCCTTCATTAACAAGAAATCTCAAGACCACAATGTGTTTGGTTCCTCCAGGCTCGGTCTTATGATCATCGCTGGACTACCTGCGGTTCTCGGTTCCCTACTTATCCTGCTTCTAGTATTCCTACTGATAAAGGTTTGTACAGGAAGAAATATTCAAAAATCTAGTGGTTGGAGGCTCACATTGTTCCAGCAGCTCGACCTCAAAGTCGAGGACTTGCTACAGTGCATAAAGCAAGAGAACATAATAGGCAAAGGCGGTGCTGGGATTGTCTACCGGGGAACCATGCCAAGCGGCCTTGACATAGCAATCAAACAGCTAGTAGGATCATCAAGTAATAGAGGGCAAAGGGATCATGGATTTGCAGCGGAGATTAAGACGCTGGGGCAAATCAAACACCGAAACATAGTGAGGCTTTTGGGACACATGTCGAACAATAAGGACTCTAATTTGTTGTTGTACGAGTACATGCCTAATGGTAGCTTGGGAAAATTATTGCATGGGGAAAATGGAGGGGAACTGCAGTGGGAGAGGAGGTATAAAATTGCAGTGGAGGCTGCTAAGGGATTGTGTTATCTACACCATGACTGTTCACCCCTGATTATACACAGAGATATCAAGTCCCATAACATCTTGTTGGATTCAAACTTTGAGGCTCATGTTGCTGATTTTGGGCTCGCCAAGTACTTTCATGGAGTAGCAGCTGATTGCATGTCTTCATTTGCTGGTTCCTTCGGTTACATCGCCCCAGAGTACGGTTATACACTAAAAGTGGATGAGAAAATAGACGTGTACAGTTTTGGTGTGGTTTTGCTGGAGCTAATAACAGGGAGGAAAGCAGTGATGAACTATGAAGACGCTATAAGCTTAGTGGGTTGGGTGAGGAAAACTACCAAGTCGTTGTCCAGTTCATCATCGGTACTAGCAGTGGTGGATCCAAATCTCGCCGTATACCCCTTGGCGGCTGCCGTACACTTGTTCAAACTGGCAATGCTTTGTGTCGACAACGACAGCACTGCAAGACCTACGATGAGGGAAGTCGTTTGCCTGATCAATCCCGTAGATGCTGCAGGGCAATATGTTTCAGGGGATAGGATTCACTTCATTGACTTTGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

988

Amino Acids

108.23

Weight (kDa)

8.58

Isoelectric Point (pI)

32.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	28 - 62	6.3e-07	Leucine rich repeat N-terminal domain
LRR_14	PF23598	143 - 272	4.8e-07	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	693 - 963	3.4e-39	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	694 - 963	4.5e-41	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1749
AasI	GACNNNNNNGTC	2 cut(s)	1094, 2051
Acc36I	ACCTGC	1 cut(s)	1936
AccI	GTMKAC	3 cut(s)	2118, 2796, 2847
AccII	CGCG	2 cut(s)	98, 1799
AccIII	TCCGGA	2 cut(s)	567, 1100
AcoI	YGGCCR	5 cut(s)	336, 738, 1066, 1210, 1690
AcsI	RAATTY	4 cut(s)	14, 790, 1132, 1551
AcuI	CTGAAG	3 cut(s)	125, 367, 950
AcyI	GRCGYC	1 cut(s)	441
AdeI	CACNNNGTG	1 cut(s)	1882
AfiI	CCNNNNNNNGG	7 cut(s)	574, 704, 1298, 1421, 1633, 2600, 2806
AflIII	ACRYGT	2 cut(s)	2271, 2634
AgeI	ACCGGT	1 cut(s)	1760
AgsI	TTSAA	6 cut(s)	364, 787, 1498, 2003, 2490, 2827
AhlI	ACTAGT	1 cut(s)	1651
AjiI	CACGTC	1 cut(s)	2635
AjnI	CCWGG	1 cut(s)	1895
AleI	CACNNNNGTG	1 cut(s)	1843
Alw21I	GWGCWC	3 cut(s)	483, 732, 1209
Alw26I	GTCTC	6 cut(s)	220, 1102, 1227, 1584, 1677, 1840
AlwNI	CAGNNNCTG	1 cut(s)	1562
Ama87I	CYCGRG	2 cut(s)	313, 413
Aor13HI	TCCGGA	2 cut(s)	567, 1100
AoxI	GGCC	9 cut(s)	117, 336, 586, 738, 1066, 1190, 1210, 1690, 2140
ApaI	GGGCCC	1 cut(s)	1194
ApeKI	GCWGC	8 cut(s)	469, 712, 2038, 2210, 2402, 2546, 2810, 2912
ApoI	RAATTY	4 cut(s)	14, 790, 1132, 1551
AsiGI	ACCGGT	1 cut(s)	1760
AspA2I	CCTAGG	1 cut(s)	661
AspLEI	GCGC	2 cut(s)	83, 100
AspS9I	GGNCC	5 cut(s)	433, 942, 974, 1190, 1191
AsuC2I	CCSGG	7 cut(s)	133, 399, 610, 620, 1117, 1713, 2123
AsuHPI	GGTGA	6 cut(s)	598, 889, 1007, 1750, 2430, 2737
AvaI	CYCGRG	2 cut(s)	313, 413
AvaII	GGWCC	3 cut(s)	433, 942, 974
AvrII	CCTAGG	1 cut(s)	661
BaeGI	GKGCMC	2 cut(s)	1194, 1364
BalI	TGGCCA	4 cut(s)	338, 740, 1068, 1692
BamHI	GGATCC	3 cut(s)	1278, 1407, 2779
BanII	GRGCYC	6 cut(s)	144, 270, 483, 1194, 1209, 2523
BauI	CACGAG	4 cut(s)	195, 281, 769, 866
BbsI	GAAGAC	2 cut(s)	2553, 2706
Bbv12I	GWGCWC	3 cut(s)	483, 732, 1209
BbvCI	CCTCAGC	1 cut(s)	1154
BbvI	GCAGC	8 cut(s)	456, 699, 2050, 2222, 2389, 2558, 2797, 2899
BccI	CCATC	4 cut(s)	76, 328, 953, 1165
BceAI	ACGGC	7 cut(s)	61, 363, 610, 700, 1090, 2777, 2798
BcgI	CGANNNNNNTGC	4 cut(s)	1359, 1393, 2843, 2877
BciT130I	CCWGG	1 cut(s)	1897
BclI	TGATCA	3 cut(s)	1780, 1911, 2895
BcnI	CCSGG	7 cut(s)	133, 399, 610, 620, 1117, 1713, 2123
BcoDI	GTCTC	6 cut(s)	220, 1102, 1227, 1584, 1677, 1840
BcuI	ACTAGT	1 cut(s)	1651
BfaI	CTAG	7 cut(s)	662, 1583, 1652, 1961, 2010, 2165, 2768
BfmI	CTRYAG	3 cut(s)	2066, 2366, 2913
BfuAI	ACCTGC	1 cut(s)	1936
BglI	GCCNNNNNGGC	2 cut(s)	594, 1074
BlnI	CCTAGG	1 cut(s)	661
BmcAI	AGTACT	1 cut(s)	2531
Bme1390I	CCNGG	8 cut(s)	133, 399, 610, 620, 1117, 1713, 1897, 2123
Bme18I	GGWCC	3 cut(s)	433, 942, 974
BmeT110I	CYCGRG	2 cut(s)	313, 413
BmgBI	CACGTC	1 cut(s)	2635
BmgT120I	GGNCC	5 cut(s)	433, 942, 974, 1190, 1191
BmrFI	CCNGG	8 cut(s)	133, 399, 610, 620, 1117, 1713, 1897, 2123
BmsI	GCATC	4 cut(s)	225, 247, 1205, 2899
BoxI	GACNNNNGTC	1 cut(s)	2272
BpiI	GAAGAC	2 cut(s)	2553, 2706
BpmI	CTGGAG	6 cut(s)	192, 497, 1031, 1475, 1879, 2681
Bpu10I	CCTNAGC	3 cut(s)	1141, 1154, 2406
BpuEI	CTTGAG	4 cut(s)	95, 449, 632, 1854
BpuMI	CCSGG	7 cut(s)	133, 399, 610, 620, 1117, 1713, 2123
BsaBI	GATNNNNATC	1 cut(s)	2784
BsaHI	GRCGYC	1 cut(s)	441
BsaJI	CCNNGG	7 cut(s)	327, 397, 661, 873, 1127, 2122, 2802
BsaWI	WCCGGW	7 cut(s)	567, 697, 841, 899, 1100, 1331, 1760
BsaXI	ACNNNNNCTCC	4 cut(s)	146, 176, 2366, 2396
Bsc4I	CCNNNNNNNGG	7 cut(s)	574, 704, 1298, 1421, 1633, 2600, 2806
Bse118I	RCCGGY	2 cut(s)	488, 1760
Bse1I	ACTGG	5 cut(s)	760, 1693, 1776, 2751, 2835
Bse3DI	GCAATG	1 cut(s)	2841
Bse8I	GATNNNNATC	1 cut(s)	2784
BseAI	TCCGGA	2 cut(s)	567, 1100
BseBI	CCWGG	1 cut(s)	1897
BseDI	CCNNGG	7 cut(s)	327, 397, 661, 873, 1127, 2122, 2802
BseGI	GGATG	3 cut(s)	423, 1099, 2626
BseJI	GATNNNNATC	1 cut(s)	2784
BseLI	CCNNNNNNNGG	7 cut(s)	574, 704, 1298, 1421, 1633, 2600, 2806
BseMI	GCAATG	1 cut(s)	2841
BseMII	CTCAG	3 cut(s)	767, 936, 1145
BseNI	ACTGG	5 cut(s)	760, 1693, 1776, 2751, 2835
BseRI	GAGGAG	1 cut(s)	2392
BseSI	GKGCMC	2 cut(s)	1194, 1364
BseX3I	CGGCCG	1 cut(s)	1210
BseXI	GCAGC	8 cut(s)	456, 699, 2050, 2222, 2389, 2558, 2797, 2899
BseYI	CCCAGC	3 cut(s)	150, 2108, 2227
Bsh1236I	CGCG	2 cut(s)	98, 1799
Bsh1285I	CGRYCG	1 cut(s)	1213
BshFI	GGCC	9 cut(s)	119, 338, 588, 740, 1068, 1192, 1212, 1692, 2142
BshTI	ACCGGT	1 cut(s)	1760
BsiEI	CGRYCG	1 cut(s)	1213
BsiHKAI	GWGCWC	3 cut(s)	483, 732, 1209
BsiHKCI	CYCGRG	2 cut(s)	313, 413
BslFI	GGGAC	5 cut(s)	477, 960, 1276, 2280, 2452
BslI	CCNNNNNNNGG	7 cut(s)	574, 704, 1298, 1421, 1633, 2600, 2806
BsmAI	GTCTC	6 cut(s)	220, 1102, 1227, 1584, 1677, 1840
BsmBI	CGTCTC	3 cut(s)	220, 1584, 1840
BsmFI	GGGAC	5 cut(s)	477, 960, 1276, 2280, 2452
BsnI	GGCC	9 cut(s)	119, 338, 588, 740, 1068, 1192, 1212, 1692, 2142
BsoBI	CYCGRG	2 cut(s)	313, 413
Bsp120I	GGGCCC	1 cut(s)	1190
Bsp1286I	GDGCHC	8 cut(s)	144, 270, 483, 732, 1194, 1209, 1364, 2523
Bsp13I	TCCGGA	2 cut(s)	567, 1100
Bsp1407I	TGTACA	3 cut(s)	494, 1985, 2637
BspANI	GGCC	9 cut(s)	119, 338, 588, 740, 1068, 1192, 1212, 1692, 2142
BspCNI	CTCAG	3 cut(s)	768, 935, 1146
BspEI	TCCGGA	2 cut(s)	567, 1100
BspFNI	CGCG	2 cut(s)	98, 1799
BspMAI	CTGCAG	2 cut(s)	2370, 2917
BspMI	ACCTGC	1 cut(s)	1936
BspQI	GCTCTTC	1 cut(s)	926
BsrDI	GCAATG	1 cut(s)	2841
BsrFI	RCCGGY	2 cut(s)	488, 1760
BsrGI	TGTACA	3 cut(s)	494, 1985, 2637
BsrI	ACTGG	5 cut(s)	760, 1693, 1776, 2751, 2835
BssAI	RCCGGY	2 cut(s)	488, 1760
BssECI	CCNNGG	7 cut(s)	327, 397, 661, 873, 1127, 2122, 2802
BssNAI	GTATAC	1 cut(s)	2797
BssNI	GRCGYC	1 cut(s)	441
BssSI	CACGAG	4 cut(s)	195, 281, 769, 866
BssT1I	CCWWGG	3 cut(s)	661, 873, 2802
Bst1107I	GTATAC	1 cut(s)	2797
Bst2BI	CACGAG	4 cut(s)	195, 281, 769, 866
Bst2UI	CCWGG	1 cut(s)	1897
Bst4CI	ACNGT	8 cut(s)	636, 677, 918, 974, 2070, 2435, 2603, 2642
Bst6I	CTCTTC	1 cut(s)	926
BstACI	GRCGYC	1 cut(s)	441
BstAUI	TGTACA	3 cut(s)	494, 1985, 2637
BstC8I	GCNNGC	8 cut(s)	483, 490, 625, 742, 862, 1079, 1360, 2523
BstDEI	CTNAG	6 cut(s)	776, 922, 1141, 1154, 2406, 2713
BstDSI	CCRYGG	1 cut(s)	327
BstEII	GGTNACC	1 cut(s)	995
BstF5I	GGATG	3 cut(s)	423, 1099, 2626
BstFNI	CGCG	2 cut(s)	98, 1799
BstHHI	GCGC	2 cut(s)	83, 100
BstMAI	GTCTC	6 cut(s)	220, 1102, 1227, 1584, 1677, 1840
BstMCI	CGRYCG	1 cut(s)	1213
BstNI	CCWGG	1 cut(s)	1897
BstNSI	RCATGY	3 cut(s)	2275, 2317, 2560
BstPAI	GACNNNNGTC	1 cut(s)	2272
BstPI	GGTNACC	1 cut(s)	995
BstSCI	CCNGG	8 cut(s)	131, 397, 608, 618, 1115, 1711, 1895, 2121
BstSFI	CTRYAG	3 cut(s)	2066, 2366, 2913
BstSLI	GKGCMC	2 cut(s)	1194, 1364
BstUI	CGCG	2 cut(s)	98, 1799
BstV1I	GCAGC	8 cut(s)	456, 699, 2050, 2222, 2389, 2558, 2797, 2899
BstV2I	GAAGAC	2 cut(s)	2553, 2706
BstX2I	RGATCY	5 cut(s)	877, 1278, 1407, 1738, 2779
BstXI	CCANNNNNNTGG	2 cut(s)	334, 1201
BstYI	RGATCY	5 cut(s)	877, 1278, 1407, 1738, 2779
BstZ17I	GTATAC	1 cut(s)	2797
BstZI	CGGCCG	1 cut(s)	1210
BsuRI	GGCC	9 cut(s)	119, 338, 588, 740, 1068, 1192, 1212, 1692, 2142
BtgI	CCRYGG	1 cut(s)	327
BtgZI	GCGATG	3 cut(s)	57, 1900, 2572
BtrI	CACGTC	1 cut(s)	2635
BtsCI	GGATG	3 cut(s)	423, 1099, 2626
BtsI	GCAGTG	5 cut(s)	2375, 2400, 2691, 2778, 2859
BtsIMutI	CAGTG	9 cut(s)	753, 1686, 1783, 2075, 2375, 2400, 2691, 2778, 2859
BveI	ACCTGC	1 cut(s)	1936
Cac8I	GCNNGC	8 cut(s)	483, 490, 625, 742, 862, 1079, 1360, 2523
CaiI	CAGNNNCTG	1 cut(s)	1562
CfoI	GCGC	2 cut(s)	83, 100
Cfr10I	RCCGGY	2 cut(s)	488, 1760
Cfr13I	GGNCC	5 cut(s)	433, 942, 974, 1190, 1191
CseI	GACGC	7 cut(s)	104, 449, 1168, 1519, 1788, 2233, 2711
CspAI	ACCGGT	1 cut(s)	1760
CspCI	CAANNNNNGTGG	2 cut(s)	1525, 1560
DdeI	CTNAG	6 cut(s)	776, 922, 1141, 1154, 2406, 2713
DraIII	CACNNNGTG	1 cut(s)	1882
DrdI	GACNNNNNNGTC	2 cut(s)	1094, 2051
DseDI	GACNNNNNNGTC	2 cut(s)	1094, 2051
EaeI	YGGCCR	5 cut(s)	336, 738, 1066, 1210, 1690
EagI	CGGCCG	1 cut(s)	1210
Eam1104I	CTCTTC	1 cut(s)	926
EarI	CTCTTC	1 cut(s)	926
EciI	GGCGGA	2 cut(s)	22, 136
Ecl136II	GAGCTC	2 cut(s)	481, 1207
EclXI	CGGCCG	1 cut(s)	1210
Eco130I	CCWWGG	3 cut(s)	661, 873, 2802
Eco147I	AGGCCT	1 cut(s)	588
Eco24I	GRGCYC	6 cut(s)	144, 270, 483, 1194, 1209, 2523
Eco32I	GATATC	2 cut(s)	1795, 2461
Eco47I	GGWCC	3 cut(s)	433, 942, 974
Eco52I	CGGCCG	1 cut(s)	1210
Eco53kI	GAGCTC	2 cut(s)	481, 1207
Eco57I	CTGAAG	3 cut(s)	125, 367, 950
Eco88I	CYCGRG	2 cut(s)	313, 413
Eco91I	GGTNACC	1 cut(s)	995
EcoICRI	GAGCTC	2 cut(s)	481, 1207
EcoO109I	RGGNCCY	1 cut(s)	433
EcoO65I	GGTNACC	1 cut(s)	995
EcoRII	CCWGG	1 cut(s)	1895
EcoRV	GATATC	2 cut(s)	1795, 2461
EcoT14I	CCWWGG	3 cut(s)	661, 873, 2802
EcoT22I	ATGCAT	1 cut(s)	240
EcoT38I	GRGCYC	6 cut(s)	144, 270, 483, 1194, 1209, 2523
ErhI	CCWWGG	3 cut(s)	661, 873, 2802
Esp3I	CGTCTC	3 cut(s)	220, 1584, 1840
FaqI	GGGAC	5 cut(s)	477, 960, 1276, 2280, 2452
FauI	CCCGC	5 cut(s)	680, 1139, 1253, 1351, 1423
FbaI	TGATCA	3 cut(s)	1780, 1911, 2895
FblI	GTMKAC	3 cut(s)	2118, 2796, 2847
FokI	GGATG	3 cut(s)	430, 1106, 2633
FriOI	GRGCYC	6 cut(s)	144, 270, 483, 1194, 1209, 2523
FspBI	CTAG	7 cut(s)	662, 1583, 1652, 1961, 2010, 2165, 2768
GlaI	GCGC	2 cut(s)	82, 99
GsaI	CCCAGC	3 cut(s)	154, 2112, 2231
GsuI	CTGGAG	6 cut(s)	192, 497, 1031, 1475, 1879, 2681
HaeIII	GGCC	9 cut(s)	119, 338, 588, 740, 1068, 1192, 1212, 1692, 2142
HgaI	GACGC	7 cut(s)	104, 449, 1168, 1519, 1788, 2233, 2711
HhaI	GCGC	2 cut(s)	83, 100
Hin1I	GRCGYC	1 cut(s)	441
Hin6I	GCGC	2 cut(s)	81, 98
HinP1I	GCGC	2 cut(s)	81, 98
HincII	GTYRAC	4 cut(s)	354, 1337, 1534, 2848
HindII	GTYRAC	4 cut(s)	354, 1337, 1534, 2848
HindIII	AAGCTT	2 cut(s)	625, 2710
HpaI	GTTAAC	1 cut(s)	354
HphI	GGTGA	6 cut(s)	598, 889, 1007, 1750, 2430, 2737
Hpy188I	TCNGA	8 cut(s)	777, 925, 1131, 1170, 1238, 1395, 1665, 1677
Hpy99I	CGWCG	3 cut(s)	89, 98, 1804
HpyAV	CCTTC	6 cut(s)	55, 109, 677, 1010, 1861, 2587
HpyCH4III	ACNGT	8 cut(s)	636, 677, 918, 974, 2070, 2435, 2603, 2642
HpyCH4IV	ACGT	4 cut(s)	213, 392, 1833, 2634
HpyF3I	CTNAG	6 cut(s)	776, 922, 1141, 1154, 2406, 2713
HpySE526I	ACGT	4 cut(s)	213, 392, 1833, 2634
Hsp92I	GRCGYC	1 cut(s)	441
HspAI	GCGC	2 cut(s)	81, 98
Kpn2I	TCCGGA	2 cut(s)	567, 1100
KroI	GCCGGC	1 cut(s)	488
KroNI	GCCGGC	1 cut(s)	490
Ksp22I	TGATCA	3 cut(s)	1780, 1911, 2895
KspAI	GTTAAC	1 cut(s)	354
LguI	GCTCTTC	1 cut(s)	926
LmnI	GCTCC	9 cut(s)	139, 167, 273, 424, 478, 570, 621, 1204, 2662
Lsp1109I	GCAGC	8 cut(s)	456, 699, 2050, 2222, 2389, 2558, 2797, 2899
LweI	GCATC	4 cut(s)	225, 247, 1205, 2899
MaeI	CTAG	7 cut(s)	662, 1583, 1652, 1961, 2010, 2165, 2768
MaeII	ACGT	4 cut(s)	213, 392, 1833, 2634
MaeIII	GTNAC	4 cut(s)	677, 746, 995, 2582
MfeI	CAATTG	1 cut(s)	1419
MflI	RGATCY	5 cut(s)	877, 1278, 1407, 1738, 2779
MhlI	GDGCHC	8 cut(s)	144, 270, 483, 732, 1194, 1209, 1364, 2523
MlsI	TGGCCA	4 cut(s)	338, 740, 1068, 1692
MluNI	TGGCCA	4 cut(s)	338, 740, 1068, 1692
MlyI	GAGTC	4 cut(s)	781, 1609, 1677, 2282
MmeI	TCCRAC	5 cut(s)	1072, 1182, 1367, 1997, 2463
Mox20I	TGGCCA	4 cut(s)	338, 740, 1068, 1692
Mph1103I	ATGCAT	1 cut(s)	240
MroI	TCCGGA	2 cut(s)	567, 1100
MroNI	GCCGGC	1 cut(s)	488
MscI	TGGCCA	4 cut(s)	338, 740, 1068, 1692
MseI	TTAA	7 cut(s)	18, 210, 353, 638, 1439, 1857, 2220
MslI	CAYNNNNRTG	3 cut(s)	76, 332, 1843
Msp20I	TGGCCA	4 cut(s)	338, 740, 1068, 1692
MspA1I	CMGCKG	8 cut(s)	387, 469, 475, 744, 1260, 1358, 2213, 2549
MspR9I	CCNGG	8 cut(s)	133, 399, 610, 620, 1117, 1713, 1897, 2123
MunI	CAATTG	1 cut(s)	1419
MvaI	CCWGG	1 cut(s)	1897
MvnI	CGCG	2 cut(s)	98, 1799
NaeI	GCCGGC	1 cut(s)	490
NciI	CCSGG	7 cut(s)	133, 399, 610, 620, 1117, 1713, 2123
NgoMIV	GCCGGC	1 cut(s)	488
NmeAIII	GCCGAG	2 cut(s)	1188, 1317
NmuCI	GTSAC	1 cut(s)	995
NsiI	ATGCAT	1 cut(s)	240
NspI	RCATGY	3 cut(s)	2275, 2317, 2560
OliI	CACNNNNGTG	1 cut(s)	1843
PceI	AGGCCT	1 cut(s)	588
PciI	ACATGT	1 cut(s)	2271
PciSI	GCTCTTC	1 cut(s)	926
PcsI	WCGNNNNNNNCGW	2 cut(s)	857, 888
PdiI	GCCGGC	1 cut(s)	490
PinAI	ACCGGT	1 cut(s)	1760
PleI	GAGTC	4 cut(s)	780, 1608, 1677, 2282
PpsI	GAGTC	4 cut(s)	780, 1608, 1677, 2282
PpuMI	RGGWCCY	1 cut(s)	433
PscI	ACATGT	1 cut(s)	2271
PshAI	GACNNNNGTC	1 cut(s)	2272
PsiI	TTATAA	1 cut(s)	1749
Psp124BI	GAGCTC	2 cut(s)	483, 1209
Psp5II	RGGWCCY	1 cut(s)	433
Psp6I	CCWGG	1 cut(s)	1895
PspEI	GGTNACC	1 cut(s)	995
PspFI	CCCAGC	3 cut(s)	150, 2108, 2227
PspGI	CCWGG	1 cut(s)	1895
PspOMI	GGGCCC	1 cut(s)	1190
PspPI	GGNCC	5 cut(s)	433, 942, 974, 1190, 1191
PspPPI	RGGWCCY	1 cut(s)	433
PsrI	GAACNNNNNNTAC	2 cut(s)	953, 985
PstI	CTGCAG	2 cut(s)	2370, 2917
PstNI	CAGNNNCTG	1 cut(s)	1562
PsuI	RGATCY	5 cut(s)	877, 1278, 1407, 1738, 2779
PvuII	CAGCTG	3 cut(s)	469, 744, 2549
RseI	CAYNNNNRTG	3 cut(s)	76, 332, 1843
SacI	GAGCTC	2 cut(s)	483, 1209
SalI	GTCGAC	1 cut(s)	2846
SapI	GCTCTTC	1 cut(s)	926
SaqAI	TTAA	7 cut(s)	18, 210, 353, 638, 1439, 1857, 2220
Sau96I	GGNCC	5 cut(s)	433, 942, 974, 1190, 1191
ScaI	AGTACT	1 cut(s)	2531
SchI	GAGTC	4 cut(s)	781, 1609, 1677, 2282
ScrFI	CCNGG	8 cut(s)	133, 399, 610, 620, 1117, 1713, 1897, 2123
SduI	GDGCHC	8 cut(s)	144, 270, 483, 732, 1194, 1209, 1364, 2523
SfaNI	GCATC	4 cut(s)	225, 247, 1205, 2899
SfcI	CTRYAG	3 cut(s)	2066, 2366, 2913
SinI	GGWCC	3 cut(s)	433, 942, 974
SmiMI	CAYNNNNRTG	3 cut(s)	76, 332, 1843
SmlI	CTYRAG	4 cut(s)	110, 428, 647, 1869
SmoI	CTYRAG	4 cut(s)	110, 428, 647, 1869
SpeI	ACTAGT	1 cut(s)	1651
SseBI	AGGCCT	1 cut(s)	588
SspI	AATATT	2 cut(s)	127, 1999
SspMI	CTAG	7 cut(s)	662, 1583, 1652, 1961, 2010, 2165, 2768
SstI	GAGCTC	2 cut(s)	483, 1209
StuI	AGGCCT	1 cut(s)	588
StyD4I	CCNGG	8 cut(s)	131, 397, 608, 618, 1115, 1711, 1895, 2121
StyI	CCWWGG	3 cut(s)	661, 873, 2802
TaaI	ACNGT	8 cut(s)	636, 677, 918, 974, 2070, 2435, 2603, 2642
TaiI	ACGT	4 cut(s)	216, 395, 1836, 2637
TaqI	TCGA	9 cut(s)	93, 241, 248, 851, 1603, 2043, 2055, 2276, 2847
TaqII	GACCGA	1 cut(s)	1892
TatI	WGTACW	5 cut(s)	494, 1985, 2310, 2529, 2637
TauI	GCSGC	6 cut(s)	229, 390, 475, 488, 2142, 2810
Tru1I	TTAA	7 cut(s)	18, 210, 353, 638, 1439, 1857, 2220
Tru9I	TTAA	7 cut(s)	18, 210, 353, 638, 1439, 1857, 2220
TscAI	CASTG	9 cut(s)	760, 1693, 1783, 2075, 2375, 2400, 2691, 2778, 2866
TseFI	GTSAC	1 cut(s)	995
TseI	GCWGC	8 cut(s)	469, 712, 2038, 2210, 2402, 2546, 2810, 2912
Tsp45I	GTSAC	1 cut(s)	995
TspGWI	ACGGA	5 cut(s)	344, 437, 1100, 1188, 1719
TspRI	CASTG	9 cut(s)	760, 1693, 1783, 2075, 2375, 2400, 2691, 2778, 2866
VpaK11BI	GGWCC	3 cut(s)	433, 942, 974
XapI	RAATTY	4 cut(s)	14, 790, 1132, 1551
XceI	RCATGY	3 cut(s)	2275, 2317, 2560
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1883
XmaJI	CCTAGG	1 cut(s)	661
XmiI	GTMKAC	3 cut(s)	2118, 2796, 2847
XspI	CTAG	7 cut(s)	662, 1583, 1652, 1961, 2010, 2165, 2768
ZrmI	AGTACT	1 cut(s)	2531
Zsp2I	ATGCAT	1 cut(s)	240

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.