Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

801231 .. 804489

Gene Structure 3259 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1010700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2922 bp

ATGGTTACCTCCGGTTTCCGCCGGATTCTCCCAGTTTGTTTTGTCGTTTTACTGCTGTGCTCCGCCACCTGCGGCGGACACGGCGACCTCGACGCGCTGTTGAAGCTCAAGGCCGCCATGATCGGCCGCAAACGTTCTGGGCTCGAGGACTGGAACCCTTCGTCATCTCACTGCTCCTTTTCTGGTGTTCTATGCGACCGCGACTCCAGAGTCGTTTCTCTCAACGTCTCGAATCTTCCCCTCTACGGCACGATAGCGGCCGAGATTGGGCTGCTTGACAAGCTCGTCAACCTCACAATTGCCGGCAACAACTTCACCGGGAGGCTTCCGGCCGAGATGGCCAACCTCACCGCTCTCAAGCACCTGAACATCTCCGGCAACTTGTTCCACGGCAGCTTCCCCATCGAAATCGTGGTGGGGATGACGGAGCTGGAGGTTCTCGACGCCTACAACAACAACTTCACCGGGACACTTCCCGTCGAGCTCGTCAGTCTAAAAAACCTGAAGCACCTCCAGCTTGGCGGGAACTTCATCACCGGCGAAATTCCCGAGAATTACTCGGAGATACAGAGCTTGGAGTATTTGGGAGTCAACGGTAATATGCTCAGCGGAAGGGTCCCCGCGAGCTTGAGTCGGTTGAAGAATCTCAGGGAACTGTACGTGGGGTACTATAACAGTTACTCCGGCGGAATTCCGCCGGAGCTGGGATCGCTGAGCTCGCTGCAAATCCTCGACATGGGCAGCTGCAACCTCGTGGGCCCCATCCCCACCACTCTCAGCCTTTTGAAACACTTGCACACTCTATTTTTACAGGTGAACCGCCTCAGCGGTAGCATTCCACCGGAGCTCTCGGCGTTGAACAGGCTCATGTCTCTTGATCTCTCCATCAACGAGCTCTCCGGAGAGATACCGGAGAGCTTCTCGGAGCTCAAGAACATCACGCTCATCAATCTGTACAGGAACAATCTCTACGGTCCGATTCCAAAGTTCATCGGCGATTTTCCTCATCTCGAGGTGCTTCAAATATGGGAGAACAACTTCACATTCGAGCTGCCGGAGAATCTCGGGCGGAACGGCAGGCTTAAGGAGCTTGACGTCACCGGAAACCACCTTACCGGACTAATTCCGCGGGATTTGTGCACAGGAGGGAATTTGAAGACGGCTATTCTGATGGAGAATCACTTCTTCGGGCCGATTCCCGAGGAACTCGGGCGGTGCAATTCGCTGACGAAAATCCGAATGATGAAGAACTCTCTCAGCGGAACGATTCCAGCTGGAATTTTCAGTTTGCCAAATTTGATCATGATCGAGCTAAACGACAATTTCTTGTCCGGCGAACTTCCAGAGCAAATGTCTGGGGGAAATATTGGAATCCTCACACTTTCCGGGAATCATCTTTCCGGAAAAATTCCGCCGGCGATTGGGAATCTCAAGAGCCTGCAAACTCTTTCCCTGGAGATGAACGGATTTTCCGGCGAAATTCCGTCGGAAATCTTTGATTTAAAGTTGCTGTCGAGGATCAACATCAGCGCCAACAACCTCGGCCGCAAAATTCCGGACTCGATTTCCCGCTGCTCTTCCCTGACGTCCGCCGATCTCAGTCAAAACAATTTGGTTGGCGAAATCCCGAAAGGAATTGCGAAGCTGAAAGTCCTGAGCATTCTCAACTTCTCCCGAAACCAACTCACCGGCGAAATCCCCGCCGAAATCCGATACATGACGAGCCTCACGACTCTCGACCTCTCCGACAACAATTTCGTCGGCAGGATACCAACCGGCGGTCAGTTCCTGGTCTTCAACGACACGTCGTTTTCCGGAAACCCCCACCTCTGCTCCCCACGCAGCGTCCAGTGCCCGTTGTTCCATCGCCCTAAACCGTTCGCCACCTCCAAGATCGTTCTCATCGTCATCGGACTCTGTACGATTCTGTTATTCTTGTTCATAACGGCTTACCGGATGACTAGGAGTAAAATCCAGAACTCTCGGGCGTGGCGGCTCACCGCCTTCAAGCCCCTCGGTTTCAGAGCAGAGGATGTCCTGGAGTGCCTTAAAGAAGAGAACATCATCGGAAAAGGCGGCGCCGGGATAGTCTACCGCGGGTCAATGCCTGACGGCGTCGACGTGGCGATCAAACGGTTAGTCGGGCGAGGAACCGGGCGAAACGATCACGGTTTCTCGGCCGAGATAAAGACGCTGGGAAGAATCCGGCACCGGAATATCGTGAGGCTGTTGGGTTACGTGTCGAACAAGGACACGAATCTGCTGCTGTACGAGTACATGCCGAATGGGAGCTTGGGGGAGCTGCTGCATGGGACGAAGGGCGGGCATTTGCAGTGGGAGAGGAGGTATCGGATCGCCGTGGAGGCCGCCAAGGGACTCTGTTACCTCCACCACGACTGTTCGCCGTTGATTATTCACAGGGATGTTAAGTCCAATAATATTTTGCTGGACTCGGATTTGGAGGCCCACGTTGCAGATTTTGGGCTCGCCAAGTTCTTGCAGGATGCTGGGGCTTCTGAGTGCATGTCTTCCATTGCTGGGTCGTACGGTTACATTGCCCCCGAGTACGCTTACACACTCAAGGTTGACGAGAAAAGCGACGTGTACAGCTTCGGCGTGGTGCTGCTGGAGCTAATAGCAGGGAGGAAGCCGGTGGGGGAATTCGGAGACGGGGTGGACATAGTAAGATGGGTGAGGGAGACAACGTCGGAGCTCCCTCAGCCGTCGGATACAGCGTCAGTACTGGCGGTGGTGGACTCCAGGCTCTCCGGGTACCCGCTGGCAGGCGTGGTACACCTGTTCAAGATTGCCATGATGTGCGTCGAGGACGAGAGCACCGCCAGGCCAACGATGAGGGAAGTGGTACACATGCTCACCAATCCTCCGCCCGCCGCTCCAACTCCGAGCCTGCTCAACCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

974

Amino Acids

106.31

Weight (kDa)

6.66

Isoelectric Point (pI)

33.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 66	1.3e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	113 - 222	4.7e-07	Leucine-rich repeat region
LRR_14	PF23598	157 - 246	1.1e-06	Leucine-rich repeat region
LRR_14	PF23598	233 - 370	7.2e-08	Leucine-rich repeat region
LRR_14	PF23598	460 - 529	8.4e-06	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	685 - 956	2.1e-42	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	685 - 956	1.2e-41	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	77
AasI	GACNNNNNNGTC	2 cut(s)	209, 284
AatII	GACGTC	2 cut(s)	1098, 1589
Acc36I	ACCTGC	1 cut(s)	77
Acc65I	GGTACC	1 cut(s)	2772
AccB1I	GGYRCC	3 cut(s)	2078, 2208, 2772
AccBSI	CCGCTC	2 cut(s)	353, 2894
AccI	GTMKAC	2 cut(s)	2091, 2118
AccII	CGCG	5 cut(s)	95, 201, 623, 1129, 2097
AccIII	TCCGGA	4 cut(s)	899, 1400, 1555, 1814
AclI	AACGTT	1 cut(s)	133
AclWI	GGATC	3 cut(s)	715, 1526, 2360
AcoI	YGGCCR	6 cut(s)	124, 258, 330, 339, 1543, 2178
AcsI	RAATTY	9 cut(s)	543, 690, 1150, 1278, 1294, 1407, 1479, 1551, 2660
AcuI	CTGAAG	1 cut(s)	524
AcyI	GRCGYC	5 cut(s)	444, 1095, 1586, 2079, 2115
AfiI	CCNNNNNNNGG	8 cut(s)	245, 267, 704, 736, 1421, 1778, 2538, 2783
AflII	CTTAAG	1 cut(s)	1082
AflIII	ACRYGT	3 cut(s)	1803, 2238, 2601
AgsI	TTSAA	9 cut(s)	103, 640, 787, 859, 1022, 1156, 1798, 2008, 2803
AjiI	CACGTC	3 cut(s)	1806, 2122, 2602
AjnI	CCWGG	5 cut(s)	1452, 1788, 2037, 2759, 2840
AjuI	GAANNNNNNNTTGG	2 cut(s)	2276, 2308
AloI	GAACNNNNNNTCC	2 cut(s)	1454, 1486
Alw21I	GWGCWC	9 cut(s)	62, 486, 719, 849, 897, 930, 1142, 2715, 2837
Alw26I	GTCTC	4 cut(s)	232, 876, 2661, 2693
Alw44I	GTGCAC	1 cut(s)	1138
AlwI	GGATC	3 cut(s)	715, 1526, 2360
AlwNI	CAGNNNCTG	1 cut(s)	1789
Ama87I	CYCGRG	8 cut(s)	143, 548, 1010, 1064, 1199, 1208, 1983, 2561
Aor13HI	TCCGGA	4 cut(s)	899, 1400, 1555, 1814
ApaI	GGGCCC	1 cut(s)	761
ApaLI	GTGCAC	1 cut(s)	1138
ApoI	RAATTY	9 cut(s)	543, 690, 1150, 1278, 1294, 1407, 1479, 1551, 2660
ArsI	GACNNNNNNTTYG	4 cut(s)	1739, 1771, 2124, 2156
Asp700I	GAANNNNTTC	2 cut(s)	1037, 1181
Asp718I	GGTACC	1 cut(s)	2772
AspLEI	GCGC	3 cut(s)	97, 1532, 2081
AspS9I	GGNCC	6 cut(s)	616, 757, 758, 974, 1190, 2464
AsuC2I	CCSGG	6 cut(s)	319, 466, 1387, 2083, 2155, 2770
AvaI	CYCGRG	8 cut(s)	143, 548, 1010, 1064, 1199, 1208, 1983, 2561
AvaII	GGWCC	2 cut(s)	616, 974
BaeGI	GKGCMC	3 cut(s)	761, 1142, 1856
BaeI	ACNNNNGTAYC	2 cut(s)	2783, 2816
BalI	TGGCCA	1 cut(s)	341
BanI	GGYRCC	3 cut(s)	2078, 2208, 2772
BanII	GRGCYC	9 cut(s)	144, 486, 719, 761, 849, 897, 930, 2487, 2715
BauI	CACGAG	1 cut(s)	752
BbsI	GAAGAC	3 cut(s)	1163, 1786, 2520
Bbv12I	GWGCWC	9 cut(s)	62, 486, 719, 849, 897, 930, 1142, 2715, 2837
BbvCI	CCTCAGC	2 cut(s)	824, 2718
BccI	CCATC	7 cut(s)	331, 410, 770, 893, 1165, 1872, 2682
BcgI	CGANNNNNNTGC	2 cut(s)	2245, 2279
BciT130I	CCWGG	5 cut(s)	1454, 1790, 2039, 2761, 2842
BciVI	GTATCC	2 cut(s)	1761, 2722
BclI	TGATCA	1 cut(s)	1299
BcnI	CCSGG	6 cut(s)	319, 466, 1387, 2083, 2155, 2770
BcoDI	GTCTC	4 cut(s)	232, 876, 2661, 2693
BfaI	CTAG	1 cut(s)	1962
BfoI	RGCGCY	2 cut(s)	1533, 2082
BfrI	CTTAAG	1 cut(s)	1082
BfuAI	ACCTGC	1 cut(s)	77
BfuI	GTATCC	2 cut(s)	1761, 2722
BglI	GCCNNNNNGGC	2 cut(s)	338, 2363
BlpI	GCTNAGC	2 cut(s)	605, 713
BmcAI	AGTACT	1 cut(s)	2742
Bme18I	GGWCC	2 cut(s)	616, 974
BmeT110I	CYCGRG	8 cut(s)	143, 548, 1010, 1064, 1199, 1208, 1983, 2561
BmgBI	CACGTC	3 cut(s)	1806, 2122, 2602
BmgT120I	GGNCC	6 cut(s)	616, 757, 758, 974, 1190, 2464
BmiI	GGNNCC	9 cut(s)	155, 617, 618, 759, 760, 2080, 2152, 2210, 2774
BmrI	ACTGGG	1 cut(s)	26
BmsI	GCATC	1 cut(s)	2494
BmuI	ACTGGG	1 cut(s)	26
BpiI	GAAGAC	3 cut(s)	1163, 1786, 2520
BplI	GAGNNNNNCTC	4 cut(s)	542, 574, 905, 937
BpmI	CTGGAG	7 cut(s)	190, 452, 497, 1475, 2060, 2648, 2743
Bpu10I	CCTNAGC	3 cut(s)	824, 1655, 2718
Bpu1102I	GCTNAGC	2 cut(s)	605, 713
BpuEI	CTTGAG	6 cut(s)	92, 341, 649, 914, 1415, 2564
BpuMI	CCSGG	6 cut(s)	319, 466, 1387, 2083, 2155, 2770
BsaAI	YACGTR	2 cut(s)	661, 2239
BsaBI	GATNNNNATC	2 cut(s)	1304, 1523
BsaHI	GRCGYC	5 cut(s)	444, 1095, 1586, 2079, 2115
BsaJI	CCNNGG	9 cut(s)	388, 1127, 1200, 1452, 1540, 2014, 2095, 2358, 2370
Bsc4I	CCNNNNNNNGG	8 cut(s)	245, 267, 704, 736, 1421, 1778, 2538, 2783
Bse118I	RCCGGY	6 cut(s)	302, 536, 1414, 1688, 1775, 2650
Bse1I	ACTGG	4 cut(s)	32, 155, 1849, 2748
Bse3DI	GCAATG	2 cut(s)	2532, 2553
Bse8I	GATNNNNATC	2 cut(s)	1304, 1523
BseAI	TCCGGA	4 cut(s)	899, 1400, 1555, 1814
BseBI	CCWGG	5 cut(s)	1454, 1790, 2039, 2761, 2842
BseDI	CCNNGG	9 cut(s)	388, 1127, 1200, 1452, 1540, 2014, 2095, 2358, 2370
BseGI	GGATG	6 cut(s)	426, 762, 1962, 2038, 2428, 2509
BseJI	GATNNNNATC	2 cut(s)	1304, 1523
BseLI	CCNNNNNNNGG	8 cut(s)	245, 267, 704, 736, 1421, 1778, 2538, 2783
BseMI	GCAATG	2 cut(s)	2532, 2553
BseNI	ACTGG	4 cut(s)	32, 155, 1849, 2748
BseRI	GAGGAG	1 cut(s)	2356
BseSI	GKGCMC	3 cut(s)	761, 1142, 1856
BseX3I	CGGCCG	5 cut(s)	124, 258, 330, 1543, 2178
BseYI	CCCAGC	4 cut(s)	703, 2194, 2507, 2537
Bsh1236I	CGCG	5 cut(s)	95, 201, 623, 1129, 2097
Bsh1285I	CGRYCG	6 cut(s)	127, 199, 261, 333, 1546, 2181
BshNI	GGYRCC	3 cut(s)	2078, 2208, 2772
BsiEI	CGRYCG	6 cut(s)	127, 199, 261, 333, 1546, 2181
BsiHKAI	GWGCWC	9 cut(s)	62, 486, 719, 849, 897, 930, 1142, 2715, 2837
BsiHKCI	CYCGRG	8 cut(s)	143, 548, 1010, 1064, 1199, 1208, 1983, 2561
BsiWI	CGTACG	1 cut(s)	2544
BslFI	GGGAC	4 cut(s)	481, 602, 2326, 2388
BslI	CCNNNNNNNGG	8 cut(s)	245, 267, 704, 736, 1421, 1778, 2538, 2783
BsmAI	GTCTC	4 cut(s)	232, 876, 2661, 2693
BsmBI	CGTCTC	2 cut(s)	232, 2661
BsmFI	GGGAC	4 cut(s)	481, 602, 2326, 2388
BsmI	GAATGC	2 cut(s)	834, 1659
BsoBI	CYCGRG	8 cut(s)	143, 548, 1010, 1064, 1199, 1208, 1983, 2561
Bsp120I	GGGCCC	1 cut(s)	757
Bsp13I	TCCGGA	4 cut(s)	899, 1400, 1555, 1814
Bsp1407I	TGTACA	2 cut(s)	954, 2604
Bsp1720I	GCTNAGC	2 cut(s)	605, 713
BspEI	TCCGGA	4 cut(s)	899, 1400, 1555, 1814
BspFNI	CGCG	5 cut(s)	95, 201, 623, 1129, 2097
BspHI	TCATGA	1 cut(s)	1302
BspLI	GGNNCC	9 cut(s)	155, 617, 618, 759, 760, 2080, 2152, 2210, 2774
BspMI	ACCTGC	1 cut(s)	77
BspPI	GGATC	3 cut(s)	715, 1526, 2360
BspQI	GCTCTTC	1 cut(s)	1582
BspT107I	GGYRCC	3 cut(s)	2078, 2208, 2772
BspTI	CTTAAG	1 cut(s)	1082
BsrBI	CCGCTC	2 cut(s)	353, 2894
BsrDI	GCAATG	2 cut(s)	2532, 2553
BsrFI	RCCGGY	6 cut(s)	302, 536, 1414, 1688, 1775, 2650
BsrGI	TGTACA	2 cut(s)	954, 2604
BsrI	ACTGG	4 cut(s)	32, 155, 1849, 2748
BssAI	RCCGGY	6 cut(s)	302, 536, 1414, 1688, 1775, 2650
BssECI	CCNNGG	9 cut(s)	388, 1127, 1200, 1452, 1540, 2014, 2095, 2358, 2370
BssNI	GRCGYC	5 cut(s)	444, 1095, 1586, 2079, 2115
BssSI	CACGAG	1 cut(s)	752
BssT1I	CCWWGG	1 cut(s)	2370
Bst2BI	CACGAG	1 cut(s)	752
Bst2UI	CCWGG	5 cut(s)	1454, 1790, 2039, 2761, 2842
Bst4CI	ACNGT	9 cut(s)	596, 657, 677, 974, 1878, 2136, 2171, 2399, 2549
Bst6I	CTCTTC	2 cut(s)	1582, 2049
BstACI	GRCGYC	5 cut(s)	444, 1095, 1586, 2079, 2115
BstAFI	CTTAAG	1 cut(s)	1082
BstAUI	TGTACA	2 cut(s)	954, 2604
BstBAI	YACGTR	2 cut(s)	661, 2239
BstDSI	CCRYGG	4 cut(s)	388, 1127, 2095, 2358
BstEII	GGTNACC	1 cut(s)	4
BstF5I	GGATG	6 cut(s)	426, 762, 1962, 2038, 2428, 2509
BstFNI	CGCG	5 cut(s)	95, 201, 623, 1129, 2097
BstH2I	RGCGCY	2 cut(s)	1533, 2082
BstHHI	GCGC	3 cut(s)	97, 1532, 2081
BstMAI	GTCTC	4 cut(s)	232, 876, 2661, 2693
BstMCI	CGRYCG	6 cut(s)	127, 199, 261, 333, 1546, 2181
BstNI	CCWGG	5 cut(s)	1454, 1790, 2039, 2761, 2842
BstNSI	RCATGY	3 cut(s)	2281, 2527, 2872
BstPI	GGTNACC	1 cut(s)	4
BstSLI	GKGCMC	3 cut(s)	761, 1142, 1856
BstUI	CGCG	5 cut(s)	95, 201, 623, 1129, 2097
BstV2I	GAAGAC	3 cut(s)	1163, 1786, 2520
BstZI	CGGCCG	5 cut(s)	124, 258, 330, 1543, 2178
BsuI	GTATCC	2 cut(s)	1761, 2722
BtgI	CCRYGG	4 cut(s)	388, 1127, 2095, 2358
BtgZI	GCGATG	1 cut(s)	1850
BtrI	CACGTC	3 cut(s)	1806, 2122, 2602
BtsCI	GGATG	6 cut(s)	426, 762, 1962, 2038, 2428, 2509
BtsI	GCAGTG	2 cut(s)	169, 2339
BtsIMutI	CAGTG	3 cut(s)	169, 1856, 2339
BveI	ACCTGC	1 cut(s)	77
CaiI	CAGNNNCTG	1 cut(s)	1789
CciI	TCATGA	1 cut(s)	1302
CfoI	GCGC	3 cut(s)	97, 1532, 2081
Cfr10I	RCCGGY	6 cut(s)	302, 536, 1414, 1688, 1775, 2650
Cfr13I	GGNCC	6 cut(s)	616, 757, 758, 974, 1190, 2464
Cfr42I	CCGCGG	2 cut(s)	1130, 2098
CpoI	CGGWCCG	1 cut(s)	974
CseI	GACGC	7 cut(s)	101, 452, 1834, 2104, 2200, 2724, 2809
CspI	CGGWCCG	1 cut(s)	974
DinI	GGCGCC	1 cut(s)	2080
DraI	TTTAAA	1 cut(s)	1503
DrdI	GACNNNNNNGTC	2 cut(s)	209, 284
DseDI	GACNNNNNNGTC	2 cut(s)	209, 284
EaeI	YGGCCR	6 cut(s)	124, 258, 330, 339, 1543, 2178
EagI	CGGCCG	5 cut(s)	124, 258, 330, 1543, 2178
Eam1104I	CTCTTC	2 cut(s)	1582, 2049
EarI	CTCTTC	2 cut(s)	1582, 2049
EciI	GGCGGA	9 cut(s)	8, 52, 90, 684, 702, 1084, 1401, 1579, 2874
Ecl136II	GAGCTC	6 cut(s)	484, 717, 847, 895, 928, 2713
EclXI	CGGCCG	5 cut(s)	124, 258, 330, 1543, 2178
Eco130I	CCWWGG	1 cut(s)	2370
Eco24I	GRGCYC	9 cut(s)	144, 486, 719, 761, 849, 897, 930, 2487, 2715
Eco47I	GGWCC	2 cut(s)	616, 974
Eco52I	CGGCCG	5 cut(s)	124, 258, 330, 1543, 2178
Eco53kI	GAGCTC	6 cut(s)	484, 717, 847, 895, 928, 2713
Eco57I	CTGAAG	1 cut(s)	524
Eco88I	CYCGRG	8 cut(s)	143, 548, 1010, 1064, 1199, 1208, 1983, 2561
Eco91I	GGTNACC	1 cut(s)	4
EcoICRI	GAGCTC	6 cut(s)	484, 717, 847, 895, 928, 2713
EcoO109I	RGGNCCY	2 cut(s)	616, 758
EcoO65I	GGTNACC	1 cut(s)	4
EcoRI	GAATTC	2 cut(s)	690, 2660
EcoRII	CCWGG	5 cut(s)	1452, 1788, 2037, 2759, 2840
EcoT14I	CCWWGG	1 cut(s)	2370
EcoT38I	GRGCYC	9 cut(s)	144, 486, 719, 761, 849, 897, 930, 2487, 2715
EgeI	GGCGCC	1 cut(s)	2080
EheI	GGCGCC	1 cut(s)	2080
ErhI	CCWWGG	1 cut(s)	2370
Esp3I	CGTCTC	2 cut(s)	232, 2661
FaqI	GGGAC	4 cut(s)	481, 602, 2326, 2388
FauI	CCCGC	9 cut(s)	515, 628, 1122, 1577, 1708, 2090, 2315, 2784, 2896
FbaI	TGATCA	1 cut(s)	1299
FblI	GTMKAC	2 cut(s)	2091, 2118
FokI	GGATG	6 cut(s)	433, 749, 1969, 2045, 2435, 2516
FriOI	GRGCYC	9 cut(s)	144, 486, 719, 761, 849, 897, 930, 2487, 2715
FspBI	CTAG	1 cut(s)	1962
GlaI	GCGC	3 cut(s)	96, 1531, 2080
GsaI	CCCAGC	4 cut(s)	707, 2198, 2511, 2541
GsuI	CTGGAG	7 cut(s)	190, 452, 497, 1475, 2060, 2648, 2743
HaeII	RGCGCY	2 cut(s)	1533, 2082
HgaI	GACGC	7 cut(s)	101, 452, 1834, 2104, 2200, 2724, 2809
HhaI	GCGC	3 cut(s)	97, 1532, 2081
Hin1I	GRCGYC	5 cut(s)	444, 1095, 1586, 2079, 2115
Hin6I	GCGC	3 cut(s)	95, 1530, 2079
HinP1I	GCGC	3 cut(s)	95, 1530, 2079
HincII	GTYRAC	4 cut(s)	289, 592, 2119, 2587
HindII	GTYRAC	4 cut(s)	289, 592, 2119, 2587
HpyAV	CCTTC	4 cut(s)	168, 606, 2014, 2311
HpyCH4III	ACNGT	9 cut(s)	596, 657, 677, 974, 1878, 2136, 2171, 2399, 2549
Hsp92I	GRCGYC	5 cut(s)	444, 1095, 1586, 2079, 2115
HspAI	GCGC	3 cut(s)	95, 1530, 2079
KasI	GGCGCC	1 cut(s)	2078
KflI	GGGWCCC	1 cut(s)	616
Kpn2I	TCCGGA	4 cut(s)	899, 1400, 1555, 1814
KpnI	GGTACC	1 cut(s)	2776
KroI	GCCGGC	2 cut(s)	302, 1414
KroNI	GCCGGC	2 cut(s)	304, 1416
Ksp22I	TGATCA	1 cut(s)	1299
KspI	CCGCGG	2 cut(s)	1130, 2098
LguI	GCTCTTC	1 cut(s)	1582
LweI	GCATC	1 cut(s)	2494
MaeI	CTAG	1 cut(s)	1962
MaeIII	GTNAC	6 cut(s)	4, 677, 1096, 2234, 2381, 2549
MbiI	CCGCTC	2 cut(s)	353, 2894
MfeI	CAATTG	1 cut(s)	297
MlsI	TGGCCA	1 cut(s)	341
MluNI	TGGCCA	1 cut(s)	341
Mly113I	GGCGCC	1 cut(s)	2079
MmeI	TCCRAC	5 cut(s)	1467, 1770, 2688, 2706, 2921
Mox20I	TGGCCA	1 cut(s)	341
MreI	CGCCGGCG	1 cut(s)	1414
MroI	TCCGGA	4 cut(s)	899, 1400, 1555, 1814
MroNI	GCCGGC	2 cut(s)	302, 1414
MroXI	GAANNNNTTC	2 cut(s)	1037, 1181
MscI	TGGCCA	1 cut(s)	341
MseI	TTAA	4 cut(s)	1083, 1502, 2049, 2427
MslI	CAYNNNNRTG	1 cut(s)	2421
Msp20I	TGGCCA	1 cut(s)	341
MspA1I	CMGCKG	9 cut(s)	609, 744, 828, 1129, 1260, 1274, 1572, 2097, 2779
MspCI	CTTAAG	1 cut(s)	1082
MunI	CAATTG	1 cut(s)	297
Mva1269I	GAATGC	2 cut(s)	834, 1659
MvaI	CCWGG	5 cut(s)	1454, 1790, 2039, 2761, 2842
MvnI	CGCG	5 cut(s)	95, 201, 623, 1129, 2097
NaeI	GCCGGC	2 cut(s)	304, 1416
NarI	GGCGCC	1 cut(s)	2079
NciI	CCSGG	6 cut(s)	319, 466, 1387, 2083, 2155, 2770
NgoMIV	GCCGGC	2 cut(s)	302, 1414
NlaIV	GGNNCC	9 cut(s)	155, 617, 618, 759, 760, 2080, 2152, 2210, 2774
NmeAIII	GCCGAG	6 cut(s)	286, 358, 830, 1521, 2156, 2206
NmuCI	GTSAC	1 cut(s)	1096
NspI	RCATGY	3 cut(s)	2281, 2527, 2872
PaeR7I	CTCGAG	2 cut(s)	143, 1010
PagI	TCATGA	1 cut(s)	1302
PaqCI	CACCTGC	1 cut(s)	77
PciSI	GCTCTTC	1 cut(s)	1582
PcsI	WCGNNNNNNNCGW	8 cut(s)	87, 1314, 1727, 1743, 1902, 2118, 2595, 2732
PctI	GAATGC	2 cut(s)	834, 1659
PdiI	GCCGGC	2 cut(s)	304, 1416
PdmI	GAANNNNTTC	2 cut(s)	1037, 1181
Pfl23II	CGTACG	1 cut(s)	2544
PflFI	GACNNNGTC	2 cut(s)	2114, 2704
PfoI	TCCNGGA	2 cut(s)	1385, 2037
PluTI	GGCGCC	1 cut(s)	2082
Ppu21I	YACGTR	2 cut(s)	661, 2239
PpuMI	RGGWCCY	1 cut(s)	616
Psp124BI	GAGCTC	6 cut(s)	486, 719, 849, 897, 930, 2715
Psp1406I	AACGTT	1 cut(s)	133
Psp5II	RGGWCCY	1 cut(s)	616
Psp6I	CCWGG	5 cut(s)	1452, 1788, 2037, 2759, 2840
PspEI	GGTNACC	1 cut(s)	4
PspFI	CCCAGC	4 cut(s)	703, 2194, 2507, 2537
PspGI	CCWGG	5 cut(s)	1452, 1788, 2037, 2759, 2840
PspLI	CGTACG	1 cut(s)	2544
PspN4I	GGNNCC	9 cut(s)	155, 617, 618, 759, 760, 2080, 2152, 2210, 2774
PspOMI	GGGCCC	1 cut(s)	757
PspPI	GGNCC	6 cut(s)	616, 757, 758, 974, 1190, 2464
PspPPI	RGGWCCY	1 cut(s)	616
PspXI	VCTCGAGB	1 cut(s)	143
PsrI	GAACNNNNNNTAC	2 cut(s)	953, 985
PstNI	CAGNNNCTG	1 cut(s)	1789
PsyI	GACNNNGTC	2 cut(s)	2114, 2704
PvuII	CAGCTG	2 cut(s)	744, 1274
RseI	CAYNNNNRTG	1 cut(s)	2421
Rsr2I	CGGWCCG	1 cut(s)	974
RsrII	CGGWCCG	1 cut(s)	974
SacI	GAGCTC	6 cut(s)	486, 719, 849, 897, 930, 2715
SacII	CCGCGG	2 cut(s)	1130, 2098
SalI	GTCGAC	1 cut(s)	2117
SapI	GCTCTTC	1 cut(s)	1582
SaqAI	TTAA	4 cut(s)	1083, 1502, 2049, 2427
Sau96I	GGNCC	6 cut(s)	616, 757, 758, 974, 1190, 2464
ScaI	AGTACT	1 cut(s)	2742
SfaNI	GCATC	1 cut(s)	2494
SfiI	GGCCNNNNNGGCC	1 cut(s)	338
SfoI	GGCGCC	1 cut(s)	2080
Sfr274I	CTCGAG	2 cut(s)	143, 1010
Sfr303I	CCGCGG	2 cut(s)	1130, 2098
SgrAI	CRCCGGYG	3 cut(s)	536, 1414, 1688
SgrBI	CCGCGG	2 cut(s)	1130, 2098
SgrDI	CGTCGACG	1 cut(s)	2117
SinI	GGWCC	2 cut(s)	616, 974
SlaI	CTCGAG	2 cut(s)	143, 1010
SmiMI	CAYNNNNRTG	1 cut(s)	2421
SmlI	CTYRAG	9 cut(s)	107, 143, 356, 628, 929, 1010, 1082, 1430, 2579
SmoI	CTYRAG	9 cut(s)	107, 143, 356, 628, 929, 1010, 1082, 1430, 2579
SspDI	GGCGCC	1 cut(s)	2078
SspI	AATATT	2 cut(s)	1366, 2440
SspMI	CTAG	1 cut(s)	1962
SstI	GAGCTC	6 cut(s)	486, 719, 849, 897, 930, 2715
StyI	CCWWGG	1 cut(s)	2370
TaaI	ACNGT	9 cut(s)	596, 657, 677, 974, 1878, 2136, 2171, 2399, 2549
TatI	WGTACW	4 cut(s)	954, 2274, 2604, 2740
TauI	GCSGC	9 cut(s)	75, 116, 129, 260, 1548, 1996, 2079, 2369, 2894
Tru1I	TTAA	4 cut(s)	1083, 1502, 2049, 2427
Tru9I	TTAA	4 cut(s)	1083, 1502, 2049, 2427
TscAI	CASTG	3 cut(s)	176, 1856, 2339
TseFI	GTSAC	1 cut(s)	1096
Tsp45I	GTSAC	1 cut(s)	1096
TspDTI	ATGAA	5 cut(s)	520, 979, 1259, 1475, 1930
TspGWI	ACGGA	3 cut(s)	440, 1473, 1479
TspRI	CASTG	3 cut(s)	176, 1856, 2339
Tth111I	GACNNNGTC	2 cut(s)	2114, 2704
Vha464I	CTTAAG	1 cut(s)	1082
VneI	GTGCAC	1 cut(s)	1138
VpaK11BI	GGWCC	2 cut(s)	616, 974
XapI	RAATTY	9 cut(s)	543, 690, 1150, 1278, 1294, 1407, 1479, 1551, 2660
XceI	RCATGY	3 cut(s)	2281, 2527, 2872
XcmI	CCANNNNNNNNNTGG	1 cut(s)	409
XhoI	CTCGAG	2 cut(s)	143, 1010
XmiI	GTMKAC	2 cut(s)	2091, 2118
XmnI	GAANNNNTTC	2 cut(s)	1037, 1181
XspI	CTAG	1 cut(s)	1962
ZraI	GACGTC	2 cut(s)	1096, 1587
ZrmI	AGTACT	1 cut(s)	2742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.