Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

34085079 .. 34088177

Gene Structure 3099 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG298200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGGTACCGGTAGGCTCCCGTTTTTGGCATAATCTGCTCCACATTTCTTTAATTTTCCTTTTCCTTCCACAATGTTGTTTCTGCGTTGGTGACCTTGACGCACTGTTGATGCTCAAGAAAGCCATGATTGGCCCCAAAGGTTCAGGGCTAGAGAACTGGGATCCCTCTTCTCATTACTACTGCTCCTTCTCTGGAGTTTCATGTGACCACCAATCACGAGTTGTTGCTCTCAACGTGTCCGGTTTGCCTCTGTTTGGGTCGATTCCAGCAGAGATTGGGCTTCTGAACAAACTCGTCAACCTCACCATTGCCGACAACAACTTCACAGGGAAGCTTCCCCTCGAGATGGCCAACCTGACTTGTTTAAAGCTCTTGAACATCTCCAACAACTTGTTCGTTGGAAAATTTCCCGGAGAAATGACGCTCAGAATGACAGAGCTTGAGGTTCTTGACGCCTATAATAACAACTATAATGGCCCACTTCCGGTAGAGCTCGTCAATCTCAAAAAGCTCAAATATCTGCATCTTGGCGGGAACTACTTCACTGGAGAAATTCCGAATAGTTATTCAGAGATTCAAAGCTTGGAGTATCTGGGCCTCAACGGTATCGGGCTCAACGGAATGTTCCCTTCCAGCTTGGCTCGGCTAAAGAATCTGAAAGAATTGTACGTTGGTTACTACAATACTTATAGTGGTGGAATTCCACCTGAGTTGGGAAGGATGGAGTCCTTGCAAGTGCTTGACATGGCCAGCTCCAACCTCACCGGCACGATTCCGGTCAGTCTAGGCTTTTTGAAGAACTTACGCCGGTTGTATCTACAGATTAACTGTTTCAGTGGTTATATACCTCCGCAACTTTCATGTTTGTTGAGTCTGACGTCTTTGGATCTCTCTATAAACCAACTCACCGGAGAAATACCAGAAAGTCTCTCAGAGCTCAAGAACCTCACCCTACTCAACCTGTATAAGAACAATTTTTACGGCCCGATACCCAACTTCATCGGCGATCTTCCTCATCTGGAGGTGCTTCAACTTTGGGAGAACAACTTTGCATATGAACTACCTGAGAGTCTTGGCCGGAATGGAAGGCTCACAGACCTCGATGTAACCGGTAGCCACCTTACCGGGTTGATTCCTCGAGATTTGTGCAAAGGAGGAAGGTTGAGGACGTTGATTCTGATGGATAATCACTTCTTTGGGCCGATTCCGGAGGCATTGGGTCGGTGCAAGTCACTGGTCAAAATCAGATTGGGGAAAAACACACTCAACGGGACTATTCCTGCCGGAATCTTCAGCTTACCTCATGTGAACATAATTGAGATCAATGATAATTACTTGTCCGGAGAGCTCCCGATGCAGCTGTCTGGTACCTCACTCGGAATCCTCACTCTTTCTGGTAATCAAATTTCTGGAAGAATCCCATCTACCATTGGAAATCTCCGGAATTTACAAATTCTTTCACTAGATATGAACAGATTCTCTGGGCCAGTACCAGAGGAAATCTTCAGTTTACAATTGCTAGCGAAACTCAACATCAGCACCAACAATTTTGGTGGTGAAATTCCGGCTTCAATCTCTGGTTGTTCCTCTCTAACTTTCCTAGATTTTAGTCAAAACACTTTTGTTGGGGAAATCCCTAGAGGGATAGGCAAACTGACAGTGCTTGGCATTCTTAATTTGTCAAGGAACAACCTGACAGGTCCAATACCTGTGGAATTCGAAGACATGGCGAGCCTCAAAACTTTGGATCTCTCCTATAACAATTTCATTGGTAGGATCCCAACCGGAGGTCACTTTTTATGGGTGGATGCTTCCTTTGAAGGAAATCCCAATCTGTGTTCGGCGGAAAAGTTGTTATGCAGCTCTGATAACCACCCACGACCTCGAGACCATGAAGTCATCATCGTTGGACTCACTGGAGCTCTGCTAACTGTGCTACTCATGTTGCTTCTTATATATGTAGTTATCAGGGTTTATAGGAATAGGAAGACCACAAAGATAGAGAAATGGGGCTCTTGGAAGCTAACAGTTTTCCAAGTACAGCATGATCTCAACATAGAGGATATTCTCCAGTGTCTAAAGGCAGAGAATATTATCGGCAGGGGTGGTGCTGGAGTTGTCTACCGGGGTACAATGCCAAATGGTGGTGATGTTGCAATCAAACAGCTGCAGAGGGATCAGGGATTTTCGGCTGAAATTAAAACTTTAGGACAAATCAAGCATCGTAATATAGTGCGGTTGTTGGGGTACATGTCGAACAAGGACACGAATTTGTTGCTGTACGAGTACATGCCTAACGGTAGCTTGGGACAACTGTTGCGTGGGTCTAATGGAGTGCATTTGGAGTGGGAAATCAGGTACAAGATTGCTGTGGAAGCTTCCAAGGGATTGTGTTATCTCCACCATGATTGTTCGCCTCTGATTATACACAGGGATGTAAAGTCCCATAACATCTTGCTGGACTCTAACTTTGAAGCTCATGTTTCTGATTTTGGGCTTGCCAAGTACTTTCAAGGACCGGCAGACTGCATGTCTTCACTTGCTGGTACCTTTGGTTACATTGCCCCAGAGTATGGTTGCACACTGAAAGTGGATGAGAAAATCGATGTGTACAGCTTTGGTGTGGTTCTGCTGGAACTAGTAACAGGGAAGAAACCAGTGGTGGAACTAGTCGAAGAAGCTGTGAACCTGGTAAGTTGGGTAAGGAAAACCACATCAGAAGTCCCTCAGTCATCCACTGCATCATCTGTACTTGCAGTTGTAGACTCCAGGCTCAGTGGGTTCCCATTGGCAGGTGTCGAGCATGTGTTCAAGATAGCAATGATGTGCGTTGAGAATCACAGTTCTGCAAGACCTACGATGAGGGAAGTCGTCTACTTGCTCACTAATCCTCCTCCTTCTGCTCCAGGCATGACAAACCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

974

Amino Acids

107.21

Weight (kDa)

6.44

Isoelectric Point (pI)

32.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	31 - 69	1.3e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	164 - 275	1e-11	Leucine-rich repeat region
LRR_14	PF23598	461 - 586	7.9e-10	Leucine-rich repeat region
LRR_8	PF13855	552 - 589	3e-06	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	694 - 958	8.1e-39	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	695 - 958	9.7e-43	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2783
AasI	GACNNNNNNGTC	1 cut(s)	1895
AatII	GACGTC	1 cut(s)	881
Acc36I	ACCTGC	1 cut(s)	2783
Acc65I	GGTACC	3 cut(s)	4, 1367, 2546
AccB1I	GGYRCC	3 cut(s)	4, 1367, 2546
AccB7I	CCANNNNNTGG	2 cut(s)	2144, 2573
AccI	GTMKAC	3 cut(s)	2121, 2763, 2874
AccIII	TCCGGA	3 cut(s)	1207, 1340, 1440
AciI	CCGC	4 cut(s)	531, 851, 1844, 2236
AclWI	GGATC	7 cut(s)	155, 168, 894, 1755, 1771, 1784, 2184
AcoI	YGGCCR	3 cut(s)	348, 747, 1075
AcsI	RAATTY	9 cut(s)	404, 552, 699, 1404, 1444, 1452, 1560, 1715, 2269
AcuI	CTGAAG	2 cut(s)	1276, 1489
AcyI	GRCGYC	2 cut(s)	453, 878
AfiI	CCNNNNNNNGG	9 cut(s)	24, 254, 346, 484, 713, 1787, 2144, 2573, 2792
AflIII	ACRYGT	2 cut(s)	234, 2250
AgeI	ACCGGT	2 cut(s)	7, 1109
AgsI	TTSAA	9 cut(s)	376, 578, 796, 1031, 1572, 1820, 2474, 2513, 2812
AhdI	GACNNNNNGTC	1 cut(s)	2530
AhlI	ACTAGT	2 cut(s)	2638, 2668
AjnI	CCWGG	3 cut(s)	2688, 2768, 2905
AjuI	GAANNNNNNNTTGG	4 cut(s)	377, 409, 654, 686
Alw21I	GWGCWC	4 cut(s)	495, 939, 1350, 1924
Alw26I	GTCTC	2 cut(s)	932, 1881
AlwI	GGATC	7 cut(s)	155, 168, 894, 1755, 1771, 1784, 2184
Ama87I	CYCGRG	3 cut(s)	341, 1137, 1884
Aor13HI	TCCGGA	3 cut(s)	1207, 1340, 1440
AoxI	GGCC	9 cut(s)	130, 348, 475, 595, 747, 982, 1075, 1199, 1484
ApeKI	GCWGC	3 cut(s)	1357, 1860, 2167
ApoI	RAATTY	9 cut(s)	404, 552, 699, 1404, 1444, 1452, 1560, 1715, 2269
ArsI	GACNNNNNNTTYG	2 cut(s)	2453, 2485
AsiGI	ACCGGT	2 cut(s)	7, 1109
Asp700I	GAANNNNTTC	2 cut(s)	539, 1475
Asp718I	GGTACC	3 cut(s)	4, 1367, 2546
AspS9I	GGNCC	8 cut(s)	131, 476, 595, 983, 1199, 1484, 1700, 2516
AsuC2I	CCSGG	3 cut(s)	411, 1126, 2126
AsuHPI	GGTGA	7 cut(s)	101, 295, 754, 898, 940, 1568, 2159
AsuII	TTCGAA	1 cut(s)	1719
AsuNHI	GCTAGC	1 cut(s)	1519
AvaI	CYCGRG	3 cut(s)	341, 1137, 1884
AvaII	GGWCC	2 cut(s)	1700, 2516
BalI	TGGCCA	2 cut(s)	350, 749
BamHI	GGATCC	2 cut(s)	160, 1776
BanI	GGYRCC	3 cut(s)	4, 1367, 2546
BanII	GRGCYC	6 cut(s)	495, 615, 939, 1350, 1924, 2015
BauI	CACGAG	1 cut(s)	216
BbsI	GAAGAC	3 cut(s)	1728, 1994, 2526
Bbv12I	GWGCWC	4 cut(s)	495, 939, 1350, 1924
BbvI	GCAGC	3 cut(s)	1369, 1872, 2154
BccI	CCATC	4 cut(s)	340, 715, 1174, 1429
BceAI	ACGGC	1 cut(s)	997
BcgI	CGANNNNNNTGC	4 cut(s)	1128, 1162, 2257, 2291
BciT130I	CCWGG	3 cut(s)	2690, 2770, 2907
BcnI	CCSGG	3 cut(s)	411, 1126, 2126
BcoDI	GTCTC	2 cut(s)	932, 1881
BcuI	ACTAGT	2 cut(s)	2638, 2668
BfaI	CTAG	8 cut(s)	149, 785, 1463, 1520, 1601, 1638, 2639, 2669
BfmI	CTRYAG	2 cut(s)	818, 2168
BfuAI	ACCTGC	1 cut(s)	2783
BisI	GCNGC	3 cut(s)	1358, 1861, 2168
BlsI	GCNGC	3 cut(s)	1359, 1862, 2169
BmcAI	AGTACT	1 cut(s)	2507
Bme1390I	CCNGG	6 cut(s)	411, 1126, 2126, 2690, 2770, 2907
Bme18I	GGWCC	2 cut(s)	1700, 2516
BmeRI	GACNNNNNGTC	1 cut(s)	2530
BmeT110I	CYCGRG	3 cut(s)	341, 1137, 1884
BmgT120I	GGNCC	8 cut(s)	131, 476, 595, 983, 1199, 1484, 1700, 2516
BmiI	GGNNCC	8 cut(s)	6, 16, 133, 162, 1369, 1778, 2548, 2783
BmrFI	CCNGG	6 cut(s)	411, 1126, 2126, 2690, 2770, 2907
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	6 cut(s)	99, 532, 1344, 1798, 2230, 2750
BmtI	GCTAGC	1 cut(s)	1523
BmuI	ACTGGG	1 cut(s)	166
BpiI	GAAGAC	3 cut(s)	1728, 1994, 2526
BpmI	CTGGAG	8 cut(s)	213, 567, 1040, 1938, 2054, 2133, 2752, 2889
Bpu14I	TTCGAA	1 cut(s)	1719
BpuEI	CTTGAG	3 cut(s)	98, 461, 923
BpuMI	CCSGG	3 cut(s)	411, 1126, 2126
Bsa29I	ATCGAT	1 cut(s)	2604
BsaHI	GRCGYC	2 cut(s)	453, 878
BsaI	GGTCTC	1 cut(s)	1881
BsaJI	CCNNGG	2 cut(s)	2125, 2382
BsaXI	ACNNNNNCTCC	4 cut(s)	167, 197, 2875, 2905
Bsc4I	CCNNNNNNNGG	9 cut(s)	24, 254, 346, 484, 713, 1787, 2144, 2573, 2792
Bse118I	RCCGGY	5 cut(s)	7, 764, 807, 1109, 2518
Bse1I	ACTGG	7 cut(s)	161, 550, 1239, 1487, 1921, 2071, 2657
Bse3DI	GCAATG	3 cut(s)	306, 2559, 2826
BseAI	TCCGGA	3 cut(s)	1207, 1340, 1440
BseBI	CCWGG	3 cut(s)	2690, 2770, 2907
BseCI	ATCGAT	1 cut(s)	2604
BseDI	CCNNGG	2 cut(s)	2125, 2382
BseGI	GGATG	5 cut(s)	726, 1813, 2440, 2599, 2732
BseLI	CCNNNNNNNGG	9 cut(s)	24, 254, 346, 484, 713, 1787, 2144, 2573, 2792
BseMI	GCAATG	3 cut(s)	306, 2559, 2826
BseMII	CTCAG	6 cut(s)	439, 699, 945, 1056, 2741, 2788
BseNI	ACTGG	7 cut(s)	161, 550, 1239, 1487, 1921, 2071, 2657
BseRI	GAGGAG	1 cut(s)	2883
BseXI	GCAGC	3 cut(s)	1369, 1872, 2154
BshFI	GGCC	9 cut(s)	132, 350, 477, 597, 749, 984, 1077, 1201, 1486
BshNI	GGYRCC	3 cut(s)	4, 1367, 2546
BshTI	ACCGGT	2 cut(s)	7, 1109
BshVI	ATCGAT	1 cut(s)	2604
BsiHKAI	GWGCWC	4 cut(s)	495, 939, 1350, 1924
BsiHKCI	CYCGRG	3 cut(s)	341, 1137, 1884
BslFI	GGGAC	4 cut(s)	1285, 2322, 2428, 2708
BslI	CCNNNNNNNGG	9 cut(s)	24, 254, 346, 484, 713, 1787, 2144, 2573, 2792
BsmAI	GTCTC	2 cut(s)	932, 1881
BsmFI	GGGAC	4 cut(s)	1285, 2322, 2428, 2708
BsmI	GAATGC	1 cut(s)	1668
BsnI	GGCC	9 cut(s)	132, 350, 477, 597, 749, 984, 1077, 1201, 1486
Bso31I	GGTCTC	1 cut(s)	1881
BsoBI	CYCGRG	3 cut(s)	341, 1137, 1884
Bsp119I	TTCGAA	1 cut(s)	1719
Bsp1286I	GDGCHC	6 cut(s)	495, 615, 939, 1350, 1924, 2015
Bsp13I	TCCGGA	3 cut(s)	1207, 1340, 1440
Bsp1407I	TGTACA	1 cut(s)	2610
Bsp143I	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
BspACI	CCGC	4 cut(s)	531, 851, 1844, 2236
BspANI	GGCC	9 cut(s)	132, 350, 477, 597, 749, 984, 1077, 1201, 1486
BspCNI	CTCAG	6 cut(s)	438, 700, 944, 1057, 2740, 2787
BspDI	ATCGAT	1 cut(s)	2604
BspEI	TCCGGA	3 cut(s)	1207, 1340, 1440
BspLI	GGNNCC	8 cut(s)	6, 16, 133, 162, 1369, 1778, 2548, 2783
BspMAI	CTGCAG	1 cut(s)	2172
BspMI	ACCTGC	1 cut(s)	2783
BspOI	GCTAGC	1 cut(s)	1523
BspPI	GGATC	7 cut(s)	155, 168, 894, 1755, 1771, 1784, 2184
BspT104I	TTCGAA	1 cut(s)	1719
BspT107I	GGYRCC	3 cut(s)	4, 1367, 2546
BspTNI	GGTCTC	1 cut(s)	1881
BsrDI	GCAATG	3 cut(s)	306, 2559, 2826
BsrFI	RCCGGY	5 cut(s)	7, 764, 807, 1109, 2518
BsrGI	TGTACA	1 cut(s)	2610
BsrI	ACTGG	7 cut(s)	161, 550, 1239, 1487, 1921, 2071, 2657
BssAI	RCCGGY	5 cut(s)	7, 764, 807, 1109, 2518
BssECI	CCNNGG	2 cut(s)	2125, 2382
BssMI	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
BssNI	GRCGYC	2 cut(s)	453, 878
BssSI	CACGAG	1 cut(s)	216
BssT1I	CCWWGG	1 cut(s)	2382
Bst2BI	CACGAG	1 cut(s)	216
Bst2UI	CCWGG	3 cut(s)	2690, 2770, 2907
Bst4CI	ACNGT	9 cut(s)	105, 605, 830, 1660, 1933, 2029, 2300, 2316, 2843
Bst6I	CTCTTC	1 cut(s)	172
BstACI	GRCGYC	2 cut(s)	453, 878
BstAPI	GCANNNNNTGC	1 cut(s)	34
BstAUI	TGTACA	1 cut(s)	2610
BstBI	TTCGAA	1 cut(s)	1719
BstC8I	GCNNGC	4 cut(s)	751, 1521, 1732, 2499
BstDEI	CTNAG	7 cut(s)	425, 708, 931, 1065, 2727, 2774, 2922
BstEII	GGTNACC	1 cut(s)	89
BstF5I	GGATG	5 cut(s)	726, 1813, 2440, 2599, 2732
BstKTI	GATC	8 cut(s)	163, 889, 1009, 1323, 1750, 1779, 2050, 2179
BstMAI	GTCTC	2 cut(s)	932, 1881
BstMBI	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
BstMWI	GCNNNNNNNGC	4 cut(s)	34, 1354, 1933, 2375
BstNI	CCWGG	3 cut(s)	2690, 2770, 2907
BstNSI	RCATGY	4 cut(s)	2254, 2293, 2533, 2807
BstPI	GGTNACC	1 cut(s)	89
BstSCI	CCNGG	6 cut(s)	409, 1124, 2124, 2688, 2768, 2905
BstSFI	CTRYAG	2 cut(s)	818, 2168
BstV1I	GCAGC	3 cut(s)	1369, 1872, 2154
BstV2I	GAAGAC	3 cut(s)	1728, 1994, 2526
BstX2I	RGATCY	4 cut(s)	160, 886, 1747, 1776
BstYI	RGATCY	4 cut(s)	160, 886, 1747, 1776
Bsu15I	ATCGAT	1 cut(s)	2604
BsuRI	GGCC	9 cut(s)	132, 350, 477, 597, 749, 984, 1077, 1201, 1486
BsuTUI	ATCGAT	1 cut(s)	2604
BtsCI	GGATG	5 cut(s)	726, 1813, 2440, 2599, 2732
BtsI	GCAGTG	1 cut(s)	2736
BveI	ACCTGC	1 cut(s)	2783
Cac8I	GCNNGC	4 cut(s)	751, 1521, 1732, 2499
Cfr10I	RCCGGY	5 cut(s)	7, 764, 807, 1109, 2518
Cfr13I	GGNCC	8 cut(s)	131, 476, 595, 983, 1199, 1484, 1700, 2516
ClaI	ATCGAT	1 cut(s)	2604
CseI	GACGC	3 cut(s)	107, 430, 461
CsiI	ACCWGGT	1 cut(s)	2688
CspAI	ACCGGT	2 cut(s)	7, 1109
CspCI	CAANNNNNGTGG	8 cut(s)	693, 728, 1534, 1569, 1692, 1727, 2390, 2425
DdeI	CTNAG	7 cut(s)	425, 708, 931, 1065, 2727, 2774, 2922
DpnI	GATC	8 cut(s)	162, 888, 1008, 1322, 1749, 1778, 2049, 2178
DpnII	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
DraI	TTTAAA	1 cut(s)	366
DrdI	GACNNNNNNGTC	1 cut(s)	1895
DriI	GACNNNNNGTC	1 cut(s)	2530
DseDI	GACNNNNNNGTC	1 cut(s)	1895
EaeI	YGGCCR	3 cut(s)	348, 747, 1075
Eam1104I	CTCTTC	1 cut(s)	172
Eam1105I	GACNNNNNGTC	1 cut(s)	2530
EarI	CTCTTC	1 cut(s)	172
EciI	GGCGGA	1 cut(s)	1859
Ecl136II	GAGCTC	4 cut(s)	493, 937, 1348, 1922
Eco130I	CCWWGG	1 cut(s)	2382
Eco24I	GRGCYC	6 cut(s)	495, 615, 939, 1350, 1924, 2015
Eco31I	GGTCTC	1 cut(s)	1881
Eco47I	GGWCC	2 cut(s)	1700, 2516
Eco53kI	GAGCTC	4 cut(s)	493, 937, 1348, 1922
Eco57I	CTGAAG	2 cut(s)	1276, 1489
Eco88I	CYCGRG	3 cut(s)	341, 1137, 1884
Eco91I	GGTNACC	1 cut(s)	89
EcoICRI	GAGCTC	4 cut(s)	493, 937, 1348, 1922
EcoO65I	GGTNACC	1 cut(s)	89
EcoRI	GAATTC	2 cut(s)	699, 1715
EcoRII	CCWGG	3 cut(s)	2688, 2768, 2905
EcoT14I	CCWWGG	1 cut(s)	2382
EcoT38I	GRGCYC	6 cut(s)	495, 615, 939, 1350, 1924, 2015
ErhI	CCWWGG	1 cut(s)	2382
FaqI	GGGAC	4 cut(s)	1285, 2322, 2428, 2708
FauI	CCCGC	1 cut(s)	524
FauNDI	CATATG	1 cut(s)	1054
FblI	GTMKAC	3 cut(s)	2121, 2763, 2874
Fnu4HI	GCNGC	3 cut(s)	1358, 1861, 2168
FokI	GGATG	5 cut(s)	733, 1820, 2447, 2606, 2719
FriOI	GRGCYC	6 cut(s)	495, 615, 939, 1350, 1924, 2015
Fsp4HI	GCNGC	3 cut(s)	1358, 1861, 2168
FspBI	CTAG	8 cut(s)	149, 785, 1463, 1520, 1601, 1638, 2639, 2669
GluI	GCNGC	3 cut(s)	1358, 1861, 2168
GsuI	CTGGAG	8 cut(s)	213, 567, 1040, 1938, 2054, 2133, 2752, 2889
HaeIII	GGCC	9 cut(s)	132, 350, 477, 597, 749, 984, 1077, 1201, 1486
HgaI	GACGC	3 cut(s)	107, 430, 461
Hin1I	GRCGYC	2 cut(s)	453, 878
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HindIII	AAGCTT	3 cut(s)	332, 580, 2376
HphI	GGTGA	7 cut(s)	101, 295, 754, 898, 940, 1568, 2159
Hpy166II	GTNNAC	8 cut(s)	298, 1309, 1511, 2122, 2611, 2686, 2764, 2875
Hpy8I	GTNNAC	8 cut(s)	298, 1309, 1511, 2122, 2611, 2686, 2764, 2875
HpyAV	CCTTC	8 cut(s)	74, 196, 639, 711, 1080, 1152, 1814, 2907
HpyCH4III	ACNGT	9 cut(s)	105, 605, 830, 1660, 1933, 2029, 2300, 2316, 2843
HpyCH4IV	ACGT	4 cut(s)	234, 669, 878, 1169
HpyF10VI	GCNNNNNNNGC	4 cut(s)	34, 1354, 1933, 2375
HpyF3I	CTNAG	7 cut(s)	425, 708, 931, 1065, 2727, 2774, 2922
HpySE526I	ACGT	4 cut(s)	234, 669, 878, 1169
Hsp92I	GRCGYC	2 cut(s)	453, 878
Kpn2I	TCCGGA	3 cut(s)	1207, 1340, 1440
KpnI	GGTACC	3 cut(s)	8, 1371, 2550
Kzo9I	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
LmnI	GCTCC	7 cut(s)	20, 42, 188, 758, 1353, 1919, 2908
Lsp1109I	GCAGC	3 cut(s)	1369, 1872, 2154
LweI	GCATC	6 cut(s)	99, 532, 1344, 1798, 2230, 2750
MabI	ACCWGGT	1 cut(s)	2688
MaeI	CTAG	8 cut(s)	149, 785, 1463, 1520, 1601, 1638, 2639, 2669
MaeII	ACGT	4 cut(s)	234, 669, 878, 1169
MaeIII	GTNAC	8 cut(s)	89, 203, 674, 1105, 1230, 1790, 2555, 2641
MalI	GATC	8 cut(s)	162, 888, 1008, 1322, 1749, 1778, 2049, 2178
MboI	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
MfeI	CAATTG	1 cut(s)	1514
MflI	RGATCY	4 cut(s)	160, 886, 1747, 1776
MhlI	GDGCHC	6 cut(s)	495, 615, 939, 1350, 1924, 2015
MlsI	TGGCCA	2 cut(s)	350, 749
MluNI	TGGCCA	2 cut(s)	350, 749
MlyI	GAGTC	6 cut(s)	734, 880, 1078, 1905, 2456, 2759
MmeI	TCCRAC	4 cut(s)	379, 408, 780, 1888
Mox20I	TGGCCA	2 cut(s)	350, 749
MroI	TCCGGA	3 cut(s)	1207, 1340, 1440
MroXI	GAANNNNTTC	2 cut(s)	539, 1475
MscI	TGGCCA	2 cut(s)	350, 749
MseI	TTAA	5 cut(s)	50, 365, 825, 1674, 2199
MslI	CAYNNNNRTG	1 cut(s)	2433
Msp20I	TGGCCA	2 cut(s)	350, 749
MspA1I	CMGCKG	2 cut(s)	1360, 2167
MspR9I	CCNGG	6 cut(s)	411, 1126, 2126, 2690, 2770, 2907
MunI	CAATTG	1 cut(s)	1514
Mva1269I	GAATGC	1 cut(s)	1668
MvaI	CCWGG	3 cut(s)	2690, 2770, 2907
MwoI	GCNNNNNNNGC	4 cut(s)	34, 1354, 1933, 2375
NciI	CCSGG	3 cut(s)	411, 1126, 2126
NdeI	CATATG	1 cut(s)	1054
NdeII	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
NheI	GCTAGC	1 cut(s)	1519
NlaIV	GGNNCC	8 cut(s)	6, 16, 133, 162, 1369, 1778, 2548, 2783
NmeAIII	GCCGAG	1 cut(s)	622
NmuCI	GTSAC	4 cut(s)	89, 203, 1230, 1790
NspI	RCATGY	4 cut(s)	2254, 2293, 2533, 2807
NspV	TTCGAA	1 cut(s)	1719
PaeR7I	CTCGAG	3 cut(s)	341, 1137, 1884
PaqCI	CACCTGC	1 cut(s)	2783
PciI	ACATGT	1 cut(s)	2250
PctI	GAATGC	1 cut(s)	1668
PdmI	GAANNNNTTC	2 cut(s)	539, 1475
PflMI	CCANNNNNTGG	2 cut(s)	2144, 2573
PfoI	TCCNGGA	1 cut(s)	409
PinAI	ACCGGT	2 cut(s)	7, 1109
PkrI	GCNGC	3 cut(s)	1359, 1862, 2169
PleI	GAGTC	6 cut(s)	733, 879, 1077, 1905, 2456, 2759
PpsI	GAGTC	6 cut(s)	733, 879, 1077, 1905, 2456, 2759
PscI	ACATGT	1 cut(s)	2250
Psp124BI	GAGCTC	4 cut(s)	495, 939, 1350, 1924
Psp6I	CCWGG	3 cut(s)	2688, 2768, 2905
PspEI	GGTNACC	1 cut(s)	89
PspGI	CCWGG	3 cut(s)	2688, 2768, 2905
PspN4I	GGNNCC	8 cut(s)	6, 16, 133, 162, 1369, 1778, 2548, 2783
PspPI	GGNCC	8 cut(s)	131, 476, 595, 983, 1199, 1484, 1700, 2516
PsrI	GAACNNNNNNTAC	2 cut(s)	962, 994
PstI	CTGCAG	1 cut(s)	2172
PsuI	RGATCY	4 cut(s)	160, 886, 1747, 1776
PvuII	CAGCTG	2 cut(s)	1360, 2167
RseI	CAYNNNNRTG	1 cut(s)	2433
SacI	GAGCTC	4 cut(s)	495, 939, 1350, 1924
SaqAI	TTAA	5 cut(s)	50, 365, 825, 1674, 2199
SatI	GCNGC	3 cut(s)	1358, 1861, 2168
Sau3AI	GATC	8 cut(s)	160, 886, 1006, 1320, 1747, 1776, 2047, 2176
Sau96I	GGNCC	8 cut(s)	131, 476, 595, 983, 1199, 1484, 1700, 2516
ScaI	AGTACT	1 cut(s)	2507
SchI	GAGTC	6 cut(s)	734, 880, 1078, 1905, 2456, 2759
ScrFI	CCNGG	6 cut(s)	411, 1126, 2126, 2690, 2770, 2907
SduI	GDGCHC	6 cut(s)	495, 615, 939, 1350, 1924, 2015
SexAI	ACCWGGT	1 cut(s)	2688
SfaNI	GCATC	6 cut(s)	99, 532, 1344, 1798, 2230, 2750
SfcI	CTRYAG	2 cut(s)	818, 2168
Sfr274I	CTCGAG	3 cut(s)	341, 1137, 1884
SfuI	TTCGAA	1 cut(s)	1719
SinI	GGWCC	2 cut(s)	1700, 2516
SlaI	CTCGAG	3 cut(s)	341, 1137, 1884
SmiMI	CAYNNNNRTG	1 cut(s)	2433
SmlI	CTYRAG	6 cut(s)	113, 341, 440, 938, 1137, 1884
SmoI	CTYRAG	6 cut(s)	113, 341, 440, 938, 1137, 1884
SpeI	ACTAGT	2 cut(s)	2638, 2668
SsiI	CCGC	4 cut(s)	531, 851, 1844, 2236
SspI	AATATT	1 cut(s)	2092
SspMI	CTAG	8 cut(s)	149, 785, 1463, 1520, 1601, 1638, 2639, 2669
SstI	GAGCTC	4 cut(s)	495, 939, 1350, 1924
StyD4I	CCNGG	6 cut(s)	409, 1124, 2124, 2688, 2768, 2905
StyI	CCWWGG	1 cut(s)	2382
TaaI	ACNGT	9 cut(s)	105, 605, 830, 1660, 1933, 2029, 2300, 2316, 2843
TaiI	ACGT	4 cut(s)	237, 672, 881, 1172
TatI	WGTACW	5 cut(s)	2038, 2286, 2505, 2610, 2749
Tru1I	TTAA	5 cut(s)	50, 365, 825, 1674, 2199
Tru9I	TTAA	5 cut(s)	50, 365, 825, 1674, 2199
TseFI	GTSAC	4 cut(s)	89, 203, 1230, 1790
TseI	GCWGC	3 cut(s)	1357, 1860, 2167
Tsp45I	GTSAC	4 cut(s)	89, 203, 1230, 1790
TspDTI	ATGAA	7 cut(s)	189, 849, 988, 1071, 1484, 1756, 1908
TspGWI	ACGGA	1 cut(s)	633
Van91I	CCANNNNNTGG	2 cut(s)	2144, 2573
VpaK11BI	GGWCC	2 cut(s)	1700, 2516
XapI	RAATTY	9 cut(s)	404, 552, 699, 1404, 1444, 1452, 1560, 1715, 2269
XceI	RCATGY	4 cut(s)	2254, 2293, 2533, 2807
XhoI	CTCGAG	3 cut(s)	341, 1137, 1884
XmiI	GTMKAC	3 cut(s)	2121, 2763, 2874
XmnI	GAANNNNTTC	2 cut(s)	539, 1475
XspI	CTAG	8 cut(s)	149, 785, 1463, 1520, 1601, 1638, 2639, 2669
ZraI	GACGTC	1 cut(s)	879
ZrmI	AGTACT	1 cut(s)	2507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.