Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

31363595 .. 31366627

Gene Structure 3033 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ49184

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGGCCAACCTGACTTCATTAAAGCTTTTGAACATCTCCAACAGCGACCTCAATGGGAGTTTTCCTGGAGAAATCATACTCCGAATGACGGAGCTTGAGGTTCTCGACGCCTACAACAACGACTTTTTAGGGACTCTTCCGCTTGAGATTGCCAGTCTCAAAAAGCTCAGATATCTACATCTTGGCGATAGCTTCTTCACTGGAGAAATTCCCGGAAATTATTCAAATATTCATAGTTTAGAATATTTGGCCCTCAACGATAACTTCCTTGCCGGAAAAGTTCCGGCTAGCTTGGCTGGGTTGAAGAATCTCAAGAAACTGTACGTCGGGAATGTTAGAATCTCAATAAACTGTAACAAGTTCGACGGTGGAATTCCACCTGAGTTGGGAATGTTAGAGTCGCTGCAAGTGCTTGACATGGTCGGCTGTAAGCTCTCCGGCACGATTCCGACGACTCTGGGCTTTTTGAAGAACTTACACACGTTGCATATATCGAATAACAGTTTGAGTGGTAATATACCTCCAGAACTTTCGGATATGGTAAACCTGATGAGTTTGGACCTCTCTGTTAACAAACTCACCGCTGAAATACCAGAGAGTTTCTCAAAGCTCAAGAACCTTAAACTTCTCCAACTAGTTGGGAACAACCTCTACGGCCAGATACCCAACTTCATTGGTGATCTTCCTCATCTGGAGGCGTTTGACGTTGGGGGCAACAACTTCACATTTGCTCTACCGGAGAGTCTTGGCCGGAATGAAAGACTTACGTCCCTCAAGGTATCCGGTAACCACCTTACCGGGTTGATTCCTCGGGATTTGTGCAAAGGAGGGAGGTTAAGGGAGTTGATTCTGAAGGAGAACCACTTCTTTGGGCCGATTCCGGAGGAATTGGGCCGGTGCAAGTCGCTTGTGGATATCCTATTGATGAAAAACACACTGAATGGGACTATTCCTCCTGGAATCTTCAGCTTACCTGATGTGAACGTGATCAACTTCAACGATAACCACTTGTCCGGAGAACTTCCGATGCAGATGTCGGCTGCCTCACTTGAATTCCTCACACTCACCCGGAATCAGATTTCTGGAAAAATCCCATCCACCATCGGAACCCTCAAGAATTTACAGAAGCTTGAGCTGGCTATGAACAGATTCTCCGGGCAAGTTCCAAAAGAAATATTCAGTTTACAATCGTTATACATACTCAACATGAGCACCAACAAATTCGGAGGTGAAATTCCAGCTGCAATTTCTGATTGTTCCTCTCTAACATCCCTAGATTTTAGTCAAAACAGTTTAGTTGGAGAAATCCCAAGAGGGATAGGCAAACTGAAAGTAATCGAGTTTCTTAATTTCTCAAGGAACAAGCTAACAGGTCCAATACCTGTGGAATTCATATACATGGCGAACCTCACAATTTTGGATCTCTCCTATAACAATTTCACTGGTAGGATCCCAACTGGAGGTCAGTTTTTATCAGACATCGCTTCGTTTGAAGGAAATCCACATCTGTGTTCGCCGAGAAATGTGTCATGCAGCTCCTATAACCACCCACGACCTCGAGACCATAAAGTCATCATCGCTGGACTCACTGGAGCTCTGCTACCTTTGCTACTCATGCCGCTTCTCATACATGTACTTATCTGGGTTTATAGGAATAGAAAGATCAGAATGATTGAGAGATGTGGATCTTGGAAGCTCACAGTTTTCCAAGTACAGCATGATCTCAAAATAGAGGACGTTCTCCAGTGTCTAAACGCAGAGAACATTATCGGCAGGGGCGGTGCTGGAGTTGTCTACCGGGGGACAATGCCAAACGGTGATGATGTCGCCATCAAACGGTTGAAGAGGGATCATGGATTTTCGGCTGAAATTAAAACATTGGGACAAATCAAGCACCGAAATATAGTGAGGCTGTTGGGGTACATGTCAAACAAGGACACCAATTTGTTGTTGTACGAGTACATGCCTAATGGTAGCTTGGGACAACTTTTGCAAGGGTCAAATGGAGGGCATTTGGAGTGGGAAATGAGGTACAAGATTGCTGTGGAAGCTGCAAAGGGATTGTGTTATCTCCACAATGATTGCGTGCCTCTGATTATACACAGGGATGTAAAGTCCTATAACATCTTGCTGGACTCTAATTTCGAAGCTCATATTGCTGATTTCGGGCTTGCCAAGTACTTTCAAGGACCAACAGATTGCATGTCTTCGTTTGCTGGTACCTTCGGTTATATTGCCCTAGAGTACGGTCGCACAATGAAAGTGGATGAGAAAATTGATGTGTACAGCTTTGGTGTGGTTCTGCTGGAACTAGTAACAGGGAGGAAACCAGTGGTGGAACTAGTGGAAGAAGTTGTGAACTTAGTAAGTTGGGTAAGGAAAACCACATCACAAATCCCTCAGCCATCCACTGCATCATCTGTACTCACAGTTGTGGACTCCAGGCTCAATGGGTTCCCATTGGCAAGTGTCGAGCATGTGTTCAAGATAGCAAAGATGTGCGTCGAGAATCACAGCTCTGCAAGGCCTACAATGAGGGAAGTCGTCTACTTCCTCACTAATCCTCCTTGTTCTGCTCCAGGCATGACAAACCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

865

Amino Acids

95.53

Weight (kDa)

7.85

Isoelectric Point (pI)

29.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	2 - 109	7.1e-07	Leucine-rich repeat region
LRR_14	PF23598	125 - 212	1.4e-06	Leucine-rich repeat region
LRR_8	PF13855	158 - 217	2.3e-07	Leucine rich repeat
LRR_14	PF23598	349 - 427	2.3e-06	Leucine-rich repeat region
Pkinase	PF00069	586 - 849	2.7e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	586 - 850	1.1e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1568
Acc65I	GGTACC	1 cut(s)	2219
AccB1I	GGYRCC	1 cut(s)	2219
AccI	GTMKAC	2 cut(s)	1794, 2547
AccIII	TCCGGA	2 cut(s)	880, 1013
AciI	CCGC	4 cut(s)	140, 582, 1619, 1779
AclWI	GGATC	5 cut(s)	1428, 1444, 1457, 1693, 1857
AcoI	YGGCCR	3 cut(s)	3, 655, 748
AcsI	RAATTY	7 cut(s)	207, 372, 1052, 1117, 1220, 1233, 1388
AcuI	CTGAAG	2 cut(s)	872, 949
AcyI	GRCGYC	1 cut(s)	108
AfiI	CCNNNNNNNGG	4 cut(s)	88, 386, 1460, 1836
AflIII	ACRYGT	3 cut(s)	480, 1630, 1923
AhlI	ACTAGT	3 cut(s)	634, 2311, 2341
AjnI	CCWGG	4 cut(s)	64, 955, 2441, 2578
AleI	CACNNNNGTG	2 cut(s)	1507, 2432
AloI	GAACNNNNNNTCC	2 cut(s)	1136, 1168
Alw21I	GWGCWC	2 cut(s)	1214, 1597
Alw26I	GTCTC	2 cut(s)	161, 1554
AlwI	GGATC	5 cut(s)	1428, 1444, 1457, 1693, 1857
Ama87I	CYCGRG	2 cut(s)	810, 1557
Aor13HI	TCCGGA	2 cut(s)	880, 1013
AoxI	GGCC	7 cut(s)	3, 249, 655, 748, 872, 892, 2525
ApeKI	GCWGC	5 cut(s)	403, 1040, 1241, 1533, 2051
ApoI	RAATTY	7 cut(s)	207, 372, 1052, 1117, 1220, 1233, 1388
ArsI	GACNNNNNNTTYG	2 cut(s)	2126, 2158
Asp700I	GAANNNNTTC	3 cut(s)	220, 863, 1148
Asp718I	GGTACC	1 cut(s)	2219
AspS9I	GGNCC	6 cut(s)	250, 559, 872, 892, 1373, 2189
AsuC2I	CCSGG	5 cut(s)	213, 799, 1069, 1156, 1799
AsuHPI	GGTGA	5 cut(s)	571, 689, 1057, 1241, 1829
AsuII	TTCGAA	1 cut(s)	2145
AsuNHI	GCTAGC	1 cut(s)	287
AvaI	CYCGRG	2 cut(s)	810, 1557
AvaII	GGWCC	3 cut(s)	559, 1373, 2189
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	1449
BanI	GGYRCC	1 cut(s)	2219
BanII	GRGCYC	1 cut(s)	1597
BbsI	GAAGAC	1 cut(s)	2199
Bbv12I	GWGCWC	2 cut(s)	1214, 1597
BbvCI	CCTCAGC	1 cut(s)	2400
BbvI	GCAGC	5 cut(s)	390, 1027, 1228, 1545, 2038
BccI	CCATC	4 cut(s)	1102, 1109, 1838, 2413
BceAI	ACGGC	1 cut(s)	670
BciT130I	CCWGG	4 cut(s)	66, 957, 2443, 2580
BciVI	GTATCC	1 cut(s)	790
BclI	TGATCA	1 cut(s)	987
BcnI	CCSGG	5 cut(s)	213, 799, 1069, 1156, 1799
BcoDI	GTCTC	2 cut(s)	161, 1554
BcuI	ACTAGT	3 cut(s)	634, 2311, 2341
BfaI	CTAG	6 cut(s)	288, 635, 1274, 2240, 2312, 2342
BfuI	GTATCC	1 cut(s)	790
BisI	GCNGC	6 cut(s)	404, 1041, 1242, 1534, 1619, 2052
BlsI	GCNGC	6 cut(s)	405, 1042, 1243, 1535, 1620, 2053
BmcAI	AGTACT	1 cut(s)	2180
Bme1390I	CCNGG	9 cut(s)	66, 213, 799, 957, 1069, 1156, 1799, 2443, 2580
Bme18I	GGWCC	3 cut(s)	559, 1373, 2189
BmeT110I	CYCGRG	2 cut(s)	810, 1557
BmgT120I	GGNCC	6 cut(s)	250, 559, 872, 892, 1373, 2189
BmiI	GGNNCC	4 cut(s)	1108, 1451, 2221, 2456
BmrFI	CCNGG	9 cut(s)	66, 213, 799, 957, 1069, 1156, 1799, 2443, 2580
BmsI	GCATC	2 cut(s)	1017, 2423
BmtI	GCTAGC	1 cut(s)	291
BoxI	GACNNNNGTC	1 cut(s)	766
BpiI	GAAGAC	1 cut(s)	2199
BplI	GAGNNNNNCTC	2 cut(s)	587, 619
Bpu10I	CCTNAGC	1 cut(s)	2400
Bpu14I	TTCGAA	1 cut(s)	2145
BpuEI	CTTGAG	8 cut(s)	116, 164, 296, 596, 758, 1097, 1151, 1339
BpuMI	CCSGG	5 cut(s)	213, 799, 1069, 1156, 1799
BsaBI	GATNNNNATC	1 cut(s)	1684
BsaHI	GRCGYC	1 cut(s)	108
BsaI	GGTCTC	1 cut(s)	1554
BsaJI	CCNNGG	2 cut(s)	809, 1798
BsaWI	WCCGGW	4 cut(s)	736, 782, 880, 1013
BsaXI	ACNNNNNCTCC	6 cut(s)	937, 967, 1136, 1166, 2548, 2578
Bsc4I	CCNNNNNNNGG	4 cut(s)	88, 386, 1460, 1836
Bse118I	RCCGGY	1 cut(s)	894
Bse1I	ACTGG	7 cut(s)	153, 205, 1447, 1462, 1594, 1744, 2330
Bse8I	GATNNNNATC	1 cut(s)	1684
BseAI	TCCGGA	2 cut(s)	880, 1013
BseBI	CCWGG	4 cut(s)	66, 957, 2443, 2580
BseDI	CCNNGG	2 cut(s)	809, 1798
BseGI	GGATG	5 cut(s)	1094, 1268, 2113, 2272, 2405
BseJI	GATNNNNATC	1 cut(s)	1684
BseLI	CCNNNNNNNGG	4 cut(s)	88, 386, 1460, 1836
BseMII	CTCAG	3 cut(s)	181, 372, 2414
BseNI	ACTGG	7 cut(s)	153, 205, 1447, 1462, 1594, 1744, 2330
BseXI	GCAGC	5 cut(s)	390, 1027, 1228, 1545, 2038
BseYI	CCCAGC	1 cut(s)	296
Bsh1285I	CGRYCG	1 cut(s)	2251
BshFI	GGCC	7 cut(s)	5, 251, 657, 750, 874, 894, 2527
BshNI	GGYRCC	1 cut(s)	2219
BsiEI	CGRYCG	1 cut(s)	2251
BsiHKAI	GWGCWC	2 cut(s)	1214, 1597
BsiHKCI	CYCGRG	2 cut(s)	810, 1557
BslFI	GGGAC	6 cut(s)	145, 754, 958, 1816, 1896, 1995
BslI	CCNNNNNNNGG	4 cut(s)	88, 386, 1460, 1836
BsmAI	GTCTC	2 cut(s)	161, 1554
BsmFI	GGGAC	6 cut(s)	145, 754, 958, 1816, 1896, 1995
BsnI	GGCC	7 cut(s)	5, 251, 657, 750, 874, 894, 2527
Bso31I	GGTCTC	1 cut(s)	1554
BsoBI	CYCGRG	2 cut(s)	810, 1557
Bsp119I	TTCGAA	1 cut(s)	2145
Bsp1286I	GDGCHC	2 cut(s)	1214, 1597
Bsp13I	TCCGGA	2 cut(s)	880, 1013
Bsp1407I	TGTACA	1 cut(s)	2283
Bsp143I	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
BspACI	CCGC	4 cut(s)	140, 582, 1619, 1779
BspANI	GGCC	7 cut(s)	5, 251, 657, 750, 874, 894, 2527
BspCNI	CTCAG	3 cut(s)	180, 373, 2413
BspEI	TCCGGA	2 cut(s)	880, 1013
BspLI	GGNNCC	4 cut(s)	1108, 1451, 2221, 2456
BspOI	GCTAGC	1 cut(s)	291
BspPI	GGATC	5 cut(s)	1428, 1444, 1457, 1693, 1857
BspT104I	TTCGAA	1 cut(s)	2145
BspT107I	GGYRCC	1 cut(s)	2219
BspTNI	GGTCTC	1 cut(s)	1554
BsrFI	RCCGGY	1 cut(s)	894
BsrGI	TGTACA	1 cut(s)	2283
BsrI	ACTGG	7 cut(s)	153, 205, 1447, 1462, 1594, 1744, 2330
BssAI	RCCGGY	1 cut(s)	894
BssECI	CCNNGG	2 cut(s)	809, 1798
BssMI	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
BssNI	GRCGYC	1 cut(s)	108
Bst2UI	CCWGG	4 cut(s)	66, 957, 2443, 2580
Bst6I	CTCTTC	2 cut(s)	141, 1838
BstACI	GRCGYC	1 cut(s)	108
BstAUI	TGTACA	1 cut(s)	2283
BstBI	TTCGAA	1 cut(s)	2145
BstC8I	GCNNGC	4 cut(s)	289, 1137, 2087, 2172
BstDEI	CTNAG	4 cut(s)	167, 381, 2362, 2400
BstEII	GGTNACC	1 cut(s)	785
BstF5I	GGATG	5 cut(s)	1094, 1268, 2113, 2272, 2405
BstKTI	GATC	8 cut(s)	682, 990, 1423, 1452, 1665, 1688, 1723, 1852
BstMAI	GTCTC	2 cut(s)	161, 1554
BstMBI	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
BstMCI	CGRYCG	1 cut(s)	2251
BstMWI	GCNNNNNNNGC	6 cut(s)	293, 409, 1606, 1615, 2048, 2156
BstNI	CCWGG	4 cut(s)	66, 957, 2443, 2580
BstNSI	RCATGY	5 cut(s)	1634, 1927, 1966, 2206, 2480
BstPAI	GACNNNNGTC	1 cut(s)	766
BstPI	GGTNACC	1 cut(s)	785
BstSCI	CCNGG	9 cut(s)	64, 211, 797, 955, 1067, 1154, 1797, 2441, 2578
BstV1I	GCAGC	5 cut(s)	390, 1027, 1228, 1545, 2038
BstV2I	GAAGAC	1 cut(s)	2199
BstX2I	RGATCY	3 cut(s)	1420, 1449, 1685
BstXI	CCANNNNNNTGG	2 cut(s)	638, 869
BstYI	RGATCY	3 cut(s)	1420, 1449, 1685
BsuI	GTATCC	1 cut(s)	790
BsuRI	GGCC	7 cut(s)	5, 251, 657, 750, 874, 894, 2527
BtgZI	GCGATG	2 cut(s)	1465, 1561
BtsCI	GGATG	5 cut(s)	1094, 1268, 2113, 2272, 2405
BtsI	GCAGTG	1 cut(s)	2409
BtsIMutI	CAGTG	7 cut(s)	198, 935, 1440, 1587, 1751, 2337, 2409
Cac8I	GCNNGC	4 cut(s)	289, 1137, 2087, 2172
Cfr10I	RCCGGY	1 cut(s)	894
Cfr13I	GGNCC	6 cut(s)	250, 559, 872, 892, 1373, 2189
CseI	GACGC	2 cut(s)	116, 2491
CspCI	CAANNNNNGTGG	8 cut(s)	366, 401, 1365, 1400, 2063, 2098, 2244, 2279
DdeI	CTNAG	4 cut(s)	167, 381, 2362, 2400
DpnI	GATC	8 cut(s)	681, 989, 1422, 1451, 1664, 1687, 1722, 1851
DpnII	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
DrdI	GACNNNNNNGTC	1 cut(s)	1568
DseDI	GACNNNNNNGTC	1 cut(s)	1568
EaeI	YGGCCR	3 cut(s)	3, 655, 748
Eam1104I	CTCTTC	2 cut(s)	141, 1838
EarI	CTCTTC	2 cut(s)	141, 1838
Ecl136II	GAGCTC	1 cut(s)	1595
Eco147I	AGGCCT	1 cut(s)	2527
Eco24I	GRGCYC	1 cut(s)	1597
Eco31I	GGTCTC	1 cut(s)	1554
Eco32I	GATATC	2 cut(s)	173, 916
Eco47I	GGWCC	3 cut(s)	559, 1373, 2189
Eco53kI	GAGCTC	1 cut(s)	1595
Eco57I	CTGAAG	2 cut(s)	872, 949
Eco88I	CYCGRG	2 cut(s)	810, 1557
Eco91I	GGTNACC	1 cut(s)	785
EcoICRI	GAGCTC	1 cut(s)	1595
EcoO65I	GGTNACC	1 cut(s)	785
EcoRI	GAATTC	3 cut(s)	372, 1052, 1388
EcoRII	CCWGG	4 cut(s)	64, 955, 2441, 2578
EcoRV	GATATC	2 cut(s)	173, 916
EcoT38I	GRGCYC	1 cut(s)	1597
FaqI	GGGAC	6 cut(s)	145, 754, 958, 1816, 1896, 1995
FbaI	TGATCA	1 cut(s)	987
FblI	GTMKAC	2 cut(s)	1794, 2547
Fnu4HI	GCNGC	6 cut(s)	404, 1041, 1242, 1534, 1619, 2052
FokI	GGATG	5 cut(s)	1081, 1255, 2120, 2279, 2392
FriOI	GRGCYC	1 cut(s)	1597
Fsp4HI	GCNGC	6 cut(s)	404, 1041, 1242, 1534, 1619, 2052
FspBI	CTAG	6 cut(s)	288, 635, 1274, 2240, 2312, 2342
GluI	GCNGC	6 cut(s)	404, 1041, 1242, 1534, 1619, 2052
GsaI	CCCAGC	1 cut(s)	300
HaeIII	GGCC	7 cut(s)	5, 251, 657, 750, 874, 894, 2527
HgaI	GACGC	2 cut(s)	116, 2491
Hin1I	GRCGYC	1 cut(s)	108
HincII	GTYRAC	1 cut(s)	571
HindII	GTYRAC	1 cut(s)	571
HindIII	AAGCTT	2 cut(s)	23, 1127
HpaI	GTTAAC	1 cut(s)	571
HphI	GGTGA	5 cut(s)	571, 689, 1057, 1241, 1829
Hpy166II	GTNNAC	9 cut(s)	544, 571, 982, 1184, 1795, 2284, 2359, 2437, 2548
Hpy8I	GTNNAC	9 cut(s)	544, 571, 982, 1184, 1795, 2284, 2359, 2437, 2548
Hpy99I	CGWCG	5 cut(s)	110, 329, 368, 454, 2507
HpyAV	CCTTC	3 cut(s)	847, 1487, 2233
HpyCH4IV	ACGT	6 cut(s)	324, 482, 705, 767, 984, 1737
HpyF10VI	GCNNNNNNNGC	6 cut(s)	293, 409, 1606, 1615, 2048, 2156
HpyF3I	CTNAG	4 cut(s)	167, 381, 2362, 2400
HpySE526I	ACGT	6 cut(s)	324, 482, 705, 767, 984, 1737
Hsp92I	GRCGYC	1 cut(s)	108
Kpn2I	TCCGGA	2 cut(s)	880, 1013
KpnI	GGTACC	1 cut(s)	2223
Ksp22I	TGATCA	1 cut(s)	987
KspAI	GTTAAC	1 cut(s)	571
Kzo9I	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
LmnI	GCTCC	4 cut(s)	91, 1541, 1592, 2581
Lsp1109I	GCAGC	5 cut(s)	390, 1027, 1228, 1545, 2038
LweI	GCATC	2 cut(s)	1017, 2423
MaeI	CTAG	6 cut(s)	288, 635, 1274, 2240, 2312, 2342
MaeII	ACGT	6 cut(s)	324, 482, 705, 767, 984, 1737
MaeIII	GTNAC	3 cut(s)	353, 785, 2314
MalI	GATC	8 cut(s)	681, 989, 1422, 1451, 1664, 1687, 1722, 1851
MboI	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
MboII	GAAGA	9 cut(s)	128, 187, 316, 481, 674, 955, 1855, 2199, 2360
MflI	RGATCY	3 cut(s)	1420, 1449, 1685
MhlI	GDGCHC	2 cut(s)	1214, 1597
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	7 cut(s)	127, 407, 448, 751, 1578, 2129, 2432
MmeI	TCCRAC	4 cut(s)	63, 473, 655, 1279
Mox20I	TGGCCA	1 cut(s)	5
MroI	TCCGGA	2 cut(s)	880, 1013
MroXI	GAANNNNTTC	3 cut(s)	220, 863, 1148
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	6 cut(s)	20, 570, 621, 836, 1347, 1872
MslI	CAYNNNNRTG	4 cut(s)	1397, 1507, 2106, 2432
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	584, 1241
MspR9I	CCNGG	9 cut(s)	66, 213, 799, 957, 1069, 1156, 1799, 2443, 2580
MvaI	CCWGG	4 cut(s)	66, 957, 2443, 2580
MwoI	GCNNNNNNNGC	6 cut(s)	293, 409, 1606, 1615, 2048, 2156
NciI	CCSGG	5 cut(s)	213, 799, 1069, 1156, 1799
NdeII	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
NheI	GCTAGC	1 cut(s)	287
NlaIV	GGNNCC	4 cut(s)	1108, 1451, 2221, 2456
NmeAIII	GCCGAG	1 cut(s)	1542
NspI	RCATGY	5 cut(s)	1634, 1927, 1966, 2206, 2480
NspV	TTCGAA	1 cut(s)	2145
OliI	CACNNNNGTG	2 cut(s)	1507, 2432
PaeR7I	CTCGAG	1 cut(s)	1557
PceI	AGGCCT	1 cut(s)	2527
PciI	ACATGT	2 cut(s)	1630, 1923
PcsI	WCGNNNNNNNCGW	1 cut(s)	449
PdmI	GAANNNNTTC	3 cut(s)	220, 863, 1148
PflFI	GACNNNGTC	1 cut(s)	419
PfoI	TCCNGGA	3 cut(s)	64, 211, 955
PkrI	GCNGC	6 cut(s)	405, 1042, 1243, 1535, 1620, 2053
PleI	GAGTC	7 cut(s)	127, 406, 448, 750, 1578, 2129, 2432
PpsI	GAGTC	7 cut(s)	127, 406, 448, 750, 1578, 2129, 2432
PscI	ACATGT	2 cut(s)	1630, 1923
PshAI	GACNNNNGTC	1 cut(s)	766
Psp124BI	GAGCTC	1 cut(s)	1597
Psp6I	CCWGG	4 cut(s)	64, 955, 2441, 2578
PspEI	GGTNACC	1 cut(s)	785
PspFI	CCCAGC	1 cut(s)	296
PspGI	CCWGG	4 cut(s)	64, 955, 2441, 2578
PspN4I	GGNNCC	4 cut(s)	1108, 1451, 2221, 2456
PspPI	GGNCC	6 cut(s)	250, 559, 872, 892, 1373, 2189
PsrI	GAACNNNNNNTAC	2 cut(s)	635, 667
PsuI	RGATCY	3 cut(s)	1420, 1449, 1685
PsyI	GACNNNGTC	1 cut(s)	419
PvuII	CAGCTG	1 cut(s)	1241
RseI	CAYNNNNRTG	4 cut(s)	1397, 1507, 2106, 2432
SacI	GAGCTC	1 cut(s)	1597
SaqAI	TTAA	6 cut(s)	20, 570, 621, 836, 1347, 1872
SatI	GCNGC	6 cut(s)	404, 1041, 1242, 1534, 1619, 2052
Sau3AI	GATC	8 cut(s)	679, 987, 1420, 1449, 1662, 1685, 1720, 1849
Sau96I	GGNCC	6 cut(s)	250, 559, 872, 892, 1373, 2189
ScaI	AGTACT	1 cut(s)	2180
SchI	GAGTC	7 cut(s)	127, 407, 448, 751, 1578, 2129, 2432
ScrFI	CCNGG	9 cut(s)	66, 213, 799, 957, 1069, 1156, 1799, 2443, 2580
SduI	GDGCHC	2 cut(s)	1214, 1597
SfaNI	GCATC	2 cut(s)	1017, 2423
Sfr274I	CTCGAG	1 cut(s)	1557
SfuI	TTCGAA	1 cut(s)	2145
SinI	GGWCC	3 cut(s)	559, 1373, 2189
SlaI	CTCGAG	1 cut(s)	1557
SmiMI	CAYNNNNRTG	4 cut(s)	1397, 1507, 2106, 2432
SmlI	CTYRAG	9 cut(s)	95, 143, 311, 611, 773, 1112, 1130, 1354, 1557
SmoI	CTYRAG	9 cut(s)	95, 143, 311, 611, 773, 1112, 1130, 1354, 1557
SpeI	ACTAGT	3 cut(s)	634, 2311, 2341
SseBI	AGGCCT	1 cut(s)	2527
SsiI	CCGC	4 cut(s)	140, 582, 1619, 1779
SspI	AATATT	3 cut(s)	229, 245, 1176
SspMI	CTAG	6 cut(s)	288, 635, 1274, 2240, 2312, 2342
SstI	GAGCTC	1 cut(s)	1597
StuI	AGGCCT	1 cut(s)	2527
StyD4I	CCNGG	9 cut(s)	64, 211, 797, 955, 1067, 1154, 1797, 2441, 2578
TaiI	ACGT	6 cut(s)	327, 485, 708, 770, 987, 1740
TaqI	TCGA	8 cut(s)	105, 363, 494, 1338, 1558, 2145, 2472, 2505
TatI	WGTACW	6 cut(s)	1633, 1711, 1959, 2178, 2283, 2422
TauI	GCSGC	1 cut(s)	1621
Tru1I	TTAA	6 cut(s)	20, 570, 621, 836, 1347, 1872
Tru9I	TTAA	6 cut(s)	20, 570, 621, 836, 1347, 1872
TscAI	CASTG	7 cut(s)	205, 942, 1447, 1594, 1751, 2337, 2416
TseI	GCWGC	5 cut(s)	403, 1040, 1241, 1533, 2051
TspDTI	ATGAA	8 cut(s)	6, 221, 661, 771, 941, 1157, 1381, 2273
TspGWI	ACGGA	1 cut(s)	104
TspRI	CASTG	7 cut(s)	205, 942, 1447, 1594, 1751, 2337, 2416
Tth111I	GACNNNGTC	1 cut(s)	419
VpaK11BI	GGWCC	3 cut(s)	559, 1373, 2189
XapI	RAATTY	7 cut(s)	207, 372, 1052, 1117, 1220, 1233, 1388
XceI	RCATGY	5 cut(s)	1634, 1927, 1966, 2206, 2480
XhoI	CTCGAG	1 cut(s)	1557
XmiI	GTMKAC	2 cut(s)	1794, 2547
XmnI	GAANNNNTTC	3 cut(s)	220, 863, 1148
XspI	CTAG	6 cut(s)	288, 635, 1274, 2240, 2312, 2342
ZrmI	AGTACT	1 cut(s)	2180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.