Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

30323905 .. 30327068

Gene Structure 3164 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG275700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGATTGGCCCCAAAGGTTCAGGGCTAGAGAACTGGGACCCCTCTTCTTATTACTACTGCTCCTTCTCTGGAGTTTCATGTGACCACCAATCACGAGTTGTTGCTCTCAACGTGTCCGGTTTGCCTCTGTTTGGCTCGCTTCCGGCGGAGATTGGGCTTCTGAACAAGCTCGTCAACCTCACCATTGCCAACAACAACTTAACAGGGAGTCTTCCCCGGGAGATGGCCAACCTGACTTGTTTAAAGCTCTTGAACATCTCCACGAACTTGTTCGTTGGAAGATTTCCCGGAGAAATCACGCTCAGAATGACAGAGCTTGAGGTTCTTGACGCCTGTGACAACAACTTCACTGGTCCACTTCCGGTAGAGCTCGTCAAACTCAAAAAGATCAAACATCTAAATCTTGGCGGGAACTACTTCGCCGGAGAAATTCCCGAAAGTTATTCAAAGATTCAAAGCTTAGAATATTTGGGCCTGCACGCTAACTCGCTTGGCGGAAAGTTTCCGCCCAGCTTGGCTCTGTTGCAGAATCTCAAGGAATTGTACGTTGGTTACGACAACACTTACAGCGGTGGAATTCCACCAGAGTTGGGAACGATGGAGTCGCTGCAAGTGCTTGACATGGCGAGCTGTAACCTCACCGGCACGATTCCTGTCAGTCTAGGCTTTTTGAAGAACTTACGCGCGTTGTTTCTACATATTAACCATTTGAGTGGTTATATACCTCCAAAACTTTCAGGTATGGTGAGTCTGATGTCTTTGGATCTCTCTATTAACGAACTCACCGGAGAAATACCAGAAAGTCTCTCGGAGCTCAAGAACCTTACCCTACTCAACCTGTTTAAGAACAATTTTTACGGCCCAATACCCAACTTCATCGGCGATCTTCCTCATCTGGAGGTGCTTCATCTTTGGCAGAACAACTTTACATTTGAACTACCTGAGAGTCTTGGCCGGAATGGAAGGCTCGAAGACCTCGATGTAGCCGGTAACCACCTCACCGGGTTGATTCCTCGAGATTTGTGCAAAGGAGGGAGGTTGAGGGCGTTGATTCTGATGAATAATCACTTCTTTGGGCCGATTCCGGAGGCATTGGGTCGGTGCAAGTCGCTCGGGCATATCCTATTGAAGAAAAACACACTCAACGGGACTATTCCTGCCGGAATCTTTAATAACTTACCTGATTTGATCAGGATTGAGCTGAATGATAACTACTTGTCTGGAAAACTTCCATTGCAGATGTCGGGTGCCTCACCCGAAATTCTCACACTTTCCCGGAATCAGATTTCTGGAAAAATCCCATCCACCATTGGAACCCTCAAGAATTTAAAACATCTTTCGCTAGATGGGAACAGATTCTCCGGGCAAGTACCAACAGAGATCTTCAGTTTACAATTGCTAAGTAAACTCAACATGAGCACCAACAATATTGGTGGTGAAATTCCGGCTTCAATCTCTGGTTGTTCCTCTCTGACGTTCCTAGATTTTAGTCAAAACACTTTTGTTGGGGAAATCCCAAGAGGGATAAGTAAGCTGAAAGTAATTCAGTTTCTTAATTTTTCAAGGAACAAGCTAACAGGTCCAATACCTGTGGAATTCATATACATGGGTAGCCTCACAAATTTGGATCTCTCCTATAACAATTTCATTGGTAGGATCCCAGCCGGAGGTCAGTTTTTATGGTCGAATGCTTGGTTTGAAGGAAATCCCAATCTGTGTTCGGCGGAAAAGGTGTTGTGCAGCTCTGATAACCACCCACGAGCTCGAGACCATAAAGTCATCATCGTTGGACTCACTGGAGCTCTGCTAACTTTGCTACTCATGTTGCTTCTTATATATGTAGTTATCAGGGTTTATAGGAAGAGGAAGACCACAAAGATAGAGAAATGCGGCTCTTGGAAGCTAACAGTTTTCCAAGTACAGCTCAATCTCAACATAGAAGACGTTCTCCAGTGTCTAAAAGCAGAGAACATTATCGGCAGGGGTGGTGCTGGAATTGTCTACCGGGGGACAATGCCAAATGGTGGTGATATTGCAATCAAACAGCTGCAGAGGGATCAGGGATTTTCAGCTGAAATTAAAACATTGGGACAAATCAAGCACCGAAATATAGTGCGGCTGCTGGGGTACATGTCGAACAAGGACACGAATTTGTTGCTGTACGAGTACATGCCTAATGGGAGGAAACCAGTGGTGCAACTAGCCGACGAACCTGTGAACCTAGTAACTTGGGTAAGGGAAACCACATCAGAAATCCCTCAGTCATCCAATGCATCATCTGTCCTTGCAGTTGTAGACTCTAGGCTCAGTAAGTTCCCATTGGCAGGTGTCGAGCATATGTTCAAGGTAGCGATGATGTGCGTCAATAATCACAGCTCTTCAAGACCTACAATGAGGGAAGTTGTCTACTTGCTCACTAATCCTCATTCTGTTCCAGGCATGACAAACCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

817

Amino Acids

89.84

Weight (kDa)

8.75

Isoelectric Point (pI)

35.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	123 - 236	3.3e-10	Leucine-rich repeat region
LRR_8	PF13855	297 - 357	4.8e-06	Leucine rich repeat
LRR_14	PF23598	434 - 523	4.1e-09	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	654 - 727	1.8e-08	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	655 - 728	2.1e-08	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2315
AasI	GACNNNNNNGTC	1 cut(s)	1775
Acc36I	ACCTGC	1 cut(s)	2315
AccB1I	GGYRCC	1 cut(s)	1247
AccB7I	CCANNNNNTGG	1 cut(s)	2024
AccI	GTMKAC	3 cut(s)	2001, 2295, 2406
AccII	CGCG	2 cut(s)	684, 686
AccIII	TCCGGA	1 cut(s)	1084
AciI	CCGC	8 cut(s)	146, 408, 495, 506, 570, 1724, 1890, 2116
AclWI	GGATC	5 cut(s)	771, 1635, 1651, 1664, 2064
AcoI	YGGCCR	2 cut(s)	225, 952
AcsI	RAATTY	8 cut(s)	429, 576, 1260, 1324, 1440, 1595, 1621, 2149
AcuI	CTGAAG	1 cut(s)	1369
AcyI	GRCGYC	1 cut(s)	330
AfaI	GTAC	6 cut(s)	545, 1371, 1920, 2129, 2162, 2168
AfiI	CCNNNNNNNGG	6 cut(s)	131, 223, 590, 1667, 2024, 2324
AflIII	ACRYGT	2 cut(s)	111, 2130
AjnI	CCWGG	1 cut(s)	2434
AjuI	GAANNNNNNNTTGG	2 cut(s)	531, 563
AloI	GAACNNNNNNTCC	2 cut(s)	1343, 1375
Alw21I	GWGCWC	5 cut(s)	372, 816, 1421, 1765, 1804
Alw26I	GTCTC	2 cut(s)	809, 1761
AlwI	GGATC	5 cut(s)	771, 1635, 1651, 1664, 2064
Ama87I	CYCGRG	4 cut(s)	216, 1014, 1112, 1764
Aor13HI	TCCGGA	1 cut(s)	1084
AoxI	GGCC	6 cut(s)	7, 225, 472, 859, 952, 1076
ApeKI	GCWGC	4 cut(s)	607, 1740, 2047, 2119
ApoI	RAATTY	8 cut(s)	429, 576, 1260, 1324, 1440, 1595, 1621, 2149
Asp700I	GAANNNNTTC	4 cut(s)	269, 416, 1355, 1944
AspLEI	GCGC	1 cut(s)	686
AspS9I	GGNCC	7 cut(s)	8, 37, 353, 472, 860, 1076, 1580
AsuC2I	CCSGG	7 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006
AsuHPI	GGTGA	8 cut(s)	172, 631, 757, 775, 991, 1245, 1448, 2039
AvaI	CYCGRG	4 cut(s)	216, 1014, 1112, 1764
AvaII	GGWCC	3 cut(s)	37, 353, 1580
BalI	TGGCCA	1 cut(s)	227
BamHI	GGATCC	1 cut(s)	1656
BanI	GGYRCC	1 cut(s)	1247
BanII	GRGCYC	4 cut(s)	372, 816, 1765, 1804
BauI	CACGAG	2 cut(s)	93, 1758
BbsI	GAAGAC	4 cut(s)	203, 978, 1874, 1947
Bbv12I	GWGCWC	5 cut(s)	372, 816, 1421, 1765, 1804
BbvI	GCAGC	4 cut(s)	594, 1752, 2034, 2106
BccI	CCATC	4 cut(s)	217, 592, 1309, 1340
BceAI	ACGGC	1 cut(s)	874
BcgI	CGANNNNNNTGC	6 cut(s)	1005, 1039, 1093, 1127, 2137, 2171
BciT130I	CCWGG	1 cut(s)	2436
BclI	TGATCA	1 cut(s)	1188
BcnI	CCSGG	7 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006
BcoDI	GTCTC	2 cut(s)	809, 1761
BfaI	CTAG	7 cut(s)	26, 662, 1343, 1481, 2201, 2222, 2301
BfmI	CTRYAG	1 cut(s)	2048
BfuAI	ACCTGC	1 cut(s)	2315
BglII	AGATCT	1 cut(s)	1380
BisI	GCNGC	6 cut(s)	608, 1741, 1891, 2048, 2117, 2120
BlsI	GCNGC	6 cut(s)	609, 1742, 1892, 2049, 2118, 2121
Bme1390I	CCNGG	8 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006, 2436
Bme18I	GGWCC	3 cut(s)	37, 353, 1580
BmeT110I	CYCGRG	4 cut(s)	216, 1014, 1112, 1764
BmgT120I	GGNCC	7 cut(s)	8, 37, 353, 472, 860, 1076, 1580
BmiI	GGNNCC	6 cut(s)	10, 38, 39, 1249, 1315, 1658
BmrFI	CCNGG	8 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006, 2436
BmrI	ACTGGG	1 cut(s)	43
BmsI	GCATC	1 cut(s)	2282
BmuI	ACTGGG	1 cut(s)	43
BpiI	GAAGAC	4 cut(s)	203, 978, 1874, 1947
BpmI	CTGGAG	4 cut(s)	90, 917, 1818, 1934
BpuEI	CTTGAG	4 cut(s)	338, 518, 800, 1304
BpuMI	CCSGG	7 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006
BsaHI	GRCGYC	1 cut(s)	330
BsaI	GGTCTC	1 cut(s)	1761
BsaJI	CCNNGG	3 cut(s)	215, 216, 2005
BsaWI	WCCGGW	4 cut(s)	116, 361, 785, 1084
BsaXI	ACNNNNNCTCC	4 cut(s)	44, 74, 1343, 1373
Bsc4I	CCNNNNNNNGG	6 cut(s)	131, 223, 590, 1667, 2024, 2324
Bse118I	RCCGGY	2 cut(s)	641, 986
Bse1I	ACTGG	5 cut(s)	38, 355, 1801, 1951, 2189
Bse3DI	GCAATG	2 cut(s)	183, 1232
BseAI	TCCGGA	1 cut(s)	1084
BseBI	CCWGG	1 cut(s)	2436
BseDI	CCNNGG	3 cut(s)	215, 216, 2005
BseGI	GGATG	2 cut(s)	1301, 2264
BseLI	CCNNNNNNNGG	6 cut(s)	131, 223, 590, 1667, 2024, 2324
BseMI	GCAATG	2 cut(s)	183, 1232
BseMII	CTCAG	4 cut(s)	316, 933, 2273, 2320
BseNI	ACTGG	5 cut(s)	38, 355, 1801, 1951, 2189
BseXI	GCAGC	4 cut(s)	594, 1752, 2034, 2106
BseYI	CCCAGC	3 cut(s)	509, 1660, 2122
BsgI	GTGCAG	2 cut(s)	461, 1759
Bsh1236I	CGCG	2 cut(s)	684, 686
BshFI	GGCC	6 cut(s)	9, 227, 474, 861, 954, 1078
BshNI	GGYRCC	1 cut(s)	1247
BsiHKAI	GWGCWC	5 cut(s)	372, 816, 1421, 1765, 1804
BsiHKCI	CYCGRG	4 cut(s)	216, 1014, 1112, 1764
BslFI	GGGAC	4 cut(s)	50, 1162, 2023, 2103
BslI	CCNNNNNNNGG	6 cut(s)	131, 223, 590, 1667, 2024, 2324
BsmAI	GTCTC	2 cut(s)	809, 1761
BsmFI	GGGAC	4 cut(s)	50, 1162, 2023, 2103
BsmI	GAATGC	1 cut(s)	1693
BsnI	GGCC	6 cut(s)	9, 227, 474, 861, 954, 1078
Bso31I	GGTCTC	1 cut(s)	1761
BsoBI	CYCGRG	4 cut(s)	216, 1014, 1112, 1764
Bsp1286I	GDGCHC	5 cut(s)	372, 816, 1421, 1765, 1804
Bsp13I	TCCGGA	1 cut(s)	1084
Bsp143I	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
BspACI	CCGC	8 cut(s)	146, 408, 495, 506, 570, 1724, 1890, 2116
BspANI	GGCC	6 cut(s)	9, 227, 474, 861, 954, 1078
BspCNI	CTCAG	4 cut(s)	315, 934, 2272, 2319
BspEI	TCCGGA	1 cut(s)	1084
BspFNI	CGCG	2 cut(s)	684, 686
BspLI	GGNNCC	6 cut(s)	10, 38, 39, 1249, 1315, 1658
BspMAI	CTGCAG	1 cut(s)	2052
BspMI	ACCTGC	1 cut(s)	2315
BspPI	GGATC	5 cut(s)	771, 1635, 1651, 1664, 2064
BspQI	GCTCTTC	1 cut(s)	2383
BspT107I	GGYRCC	1 cut(s)	1247
BspTNI	GGTCTC	1 cut(s)	1761
BsrDI	GCAATG	2 cut(s)	183, 1232
BsrFI	RCCGGY	2 cut(s)	641, 986
BsrI	ACTGG	5 cut(s)	38, 355, 1801, 1951, 2189
BssAI	RCCGGY	2 cut(s)	641, 986
BssECI	CCNNGG	3 cut(s)	215, 216, 2005
BssMI	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
BssNI	GRCGYC	1 cut(s)	330
BssSI	CACGAG	2 cut(s)	93, 1758
Bst2BI	CACGAG	2 cut(s)	93, 1758
Bst2UI	CCWGG	1 cut(s)	2436
Bst4CI	ACNGT	1 cut(s)	1909
Bst6I	CTCTTC	3 cut(s)	49, 1856, 2383
BstACI	GRCGYC	1 cut(s)	330
BstC8I	GCNNGC	4 cut(s)	137, 476, 480, 628
BstDEI	CTNAG	7 cut(s)	302, 460, 942, 1400, 2259, 2306, 2451
BstEII	GGTNACC	1 cut(s)	989
BstF5I	GGATG	2 cut(s)	1301, 2264
BstFNI	CGCG	2 cut(s)	684, 686
BstHHI	GCGC	1 cut(s)	686
BstKTI	GATC	8 cut(s)	390, 766, 886, 1191, 1383, 1630, 1659, 2059
BstMAI	GTCTC	2 cut(s)	809, 1761
BstMBI	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
BstMWI	GCNNNNNNNGC	2 cut(s)	613, 1813
BstNI	CCWGG	1 cut(s)	2436
BstNSI	RCATGY	2 cut(s)	2134, 2173
BstPI	GGTNACC	1 cut(s)	989
BstSCI	CCNGG	8 cut(s)	215, 216, 286, 1001, 1274, 1361, 2004, 2434
BstSFI	CTRYAG	1 cut(s)	2048
BstUI	CGCG	2 cut(s)	684, 686
BstV1I	GCAGC	4 cut(s)	594, 1752, 2034, 2106
BstV2I	GAAGAC	4 cut(s)	203, 978, 1874, 1947
BstX2I	RGATCY	4 cut(s)	763, 1380, 1627, 1656
BstXI	CCANNNNNNTGG	1 cut(s)	713
BstYI	RGATCY	4 cut(s)	763, 1380, 1627, 1656
BsuRI	GGCC	6 cut(s)	9, 227, 474, 861, 954, 1078
BtgZI	GCGATG	1 cut(s)	2366
BtsCI	GGATG	2 cut(s)	1301, 2264
BtsIMutI	CAGTG	4 cut(s)	348, 1794, 1958, 2196
BveI	ACCTGC	1 cut(s)	2315
Cac8I	GCNNGC	4 cut(s)	137, 476, 480, 628
CfoI	GCGC	1 cut(s)	686
Cfr10I	RCCGGY	2 cut(s)	641, 986
Cfr13I	GGNCC	7 cut(s)	8, 37, 353, 472, 860, 1076, 1580
Cfr9I	CCCGGG	1 cut(s)	216
CseI	GACGC	2 cut(s)	338, 2350
Csp6I	GTAC	6 cut(s)	544, 1370, 1919, 2128, 2161, 2167
CspCI	CAANNNNNGTGG	6 cut(s)	570, 605, 1414, 1449, 1572, 1607
CviAII	CATG	8 cut(s)	78, 622, 1414, 1606, 1822, 2131, 2170, 2440
CviQI	GTAC	6 cut(s)	544, 1370, 1919, 2128, 2161, 2167
DdeI	CTNAG	7 cut(s)	302, 460, 942, 1400, 2259, 2306, 2451
DpnI	GATC	8 cut(s)	389, 765, 885, 1190, 1382, 1629, 1658, 2058
DpnII	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
DraI	TTTAAA	2 cut(s)	243, 1329
DrdI	GACNNNNNNGTC	1 cut(s)	1775
DseDI	GACNNNNNNGTC	1 cut(s)	1775
EaeI	YGGCCR	2 cut(s)	225, 952
Eam1104I	CTCTTC	3 cut(s)	49, 1856, 2383
EarI	CTCTTC	3 cut(s)	49, 1856, 2383
EciI	GGCGGA	4 cut(s)	161, 495, 510, 1739
Ecl136II	GAGCTC	4 cut(s)	370, 814, 1763, 1802
Eco24I	GRGCYC	4 cut(s)	372, 816, 1765, 1804
Eco31I	GGTCTC	1 cut(s)	1761
Eco47I	GGWCC	3 cut(s)	37, 353, 1580
Eco53kI	GAGCTC	4 cut(s)	370, 814, 1763, 1802
Eco57I	CTGAAG	1 cut(s)	1369
Eco88I	CYCGRG	4 cut(s)	216, 1014, 1112, 1764
Eco91I	GGTNACC	1 cut(s)	989
EcoICRI	GAGCTC	4 cut(s)	370, 814, 1763, 1802
EcoO109I	RGGNCCY	1 cut(s)	37
EcoO65I	GGTNACC	1 cut(s)	989
EcoRI	GAATTC	2 cut(s)	576, 1595
EcoRII	CCWGG	1 cut(s)	2434
EcoT22I	ATGCAT	1 cut(s)	2275
EcoT38I	GRGCYC	4 cut(s)	372, 816, 1765, 1804
FaeI	CATG	8 cut(s)	81, 625, 1417, 1609, 1825, 2134, 2173, 2443
FaqI	GGGAC	4 cut(s)	50, 1162, 2023, 2103
FatI	CATG	8 cut(s)	77, 621, 1413, 1605, 1821, 2130, 2169, 2439
FauI	CCCGC	1 cut(s)	401
FauNDI	CATATG	1 cut(s)	2337
FbaI	TGATCA	1 cut(s)	1188
FblI	GTMKAC	3 cut(s)	2001, 2295, 2406
Fnu4HI	GCNGC	6 cut(s)	608, 1741, 1891, 2048, 2117, 2120
FokI	GGATG	2 cut(s)	1288, 2251
FriOI	GRGCYC	4 cut(s)	372, 816, 1765, 1804
Fsp4HI	GCNGC	6 cut(s)	608, 1741, 1891, 2048, 2117, 2120
FspBI	CTAG	7 cut(s)	26, 662, 1343, 1481, 2201, 2222, 2301
GlaI	GCGC	1 cut(s)	685
GluI	GCNGC	6 cut(s)	608, 1741, 1891, 2048, 2117, 2120
GsaI	CCCAGC	3 cut(s)	513, 1664, 2126
GsuI	CTGGAG	4 cut(s)	90, 917, 1818, 1934
HaeIII	GGCC	6 cut(s)	9, 227, 474, 861, 954, 1078
HgaI	GACGC	2 cut(s)	338, 2350
HhaI	GCGC	1 cut(s)	686
Hin1I	GRCGYC	1 cut(s)	330
Hin1II	CATG	8 cut(s)	81, 625, 1417, 1609, 1825, 2134, 2173, 2443
Hin6I	GCGC	1 cut(s)	684
HinP1I	GCGC	1 cut(s)	684
HincII	GTYRAC	1 cut(s)	175
HindII	GTYRAC	1 cut(s)	175
HindIII	AAGCTT	1 cut(s)	457
HphI	GGTGA	8 cut(s)	172, 631, 757, 775, 991, 1245, 1448, 2039
Hpy166II	GTNNAC	8 cut(s)	175, 356, 1391, 1406, 2002, 2218, 2296, 2407
Hpy188I	TCNGA	9 cut(s)	162, 305, 753, 811, 1056, 1284, 1473, 1747, 2251
Hpy8I	GTNNAC	8 cut(s)	175, 356, 1391, 1406, 2002, 2218, 2296, 2407
Hpy99I	CGWCG	1 cut(s)	2210
HpyAV	CCTTC	3 cut(s)	73, 957, 1694
HpyCH4III	ACNGT	1 cut(s)	1909
HpyCH4IV	ACGT	4 cut(s)	111, 546, 1475, 1944
HpyF10VI	GCNNNNNNNGC	2 cut(s)	613, 1813
HpyF3I	CTNAG	7 cut(s)	302, 460, 942, 1400, 2259, 2306, 2451
HpySE526I	ACGT	4 cut(s)	111, 546, 1475, 1944
Hsp92I	GRCGYC	1 cut(s)	330
Hsp92II	CATG	8 cut(s)	81, 625, 1417, 1609, 1825, 2134, 2173, 2443
HspAI	GCGC	1 cut(s)	684
KflI	GGGWCCC	1 cut(s)	37
Kpn2I	TCCGGA	1 cut(s)	1084
Ksp22I	TGATCA	1 cut(s)	1188
Kzo9I	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
LguI	GCTCTTC	1 cut(s)	2383
LmnI	GCTCC	3 cut(s)	65, 811, 1799
Lsp1109I	GCAGC	4 cut(s)	594, 1752, 2034, 2106
LweI	GCATC	1 cut(s)	2282
MaeI	CTAG	7 cut(s)	26, 662, 1343, 1481, 2201, 2222, 2301
MaeII	ACGT	4 cut(s)	111, 546, 1475, 1944
MaeIII	GTNAC	6 cut(s)	80, 335, 551, 632, 989, 2224
MalI	GATC	8 cut(s)	389, 765, 885, 1190, 1382, 1629, 1658, 2058
MboI	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
MfeI	CAATTG	1 cut(s)	1394
MflI	RGATCY	4 cut(s)	763, 1380, 1627, 1656
MhlI	GDGCHC	5 cut(s)	372, 816, 1421, 1765, 1804
MlsI	TGGCCA	1 cut(s)	227
MluNI	TGGCCA	1 cut(s)	227
MlyI	GAGTC	6 cut(s)	217, 611, 757, 955, 1785, 2291
MmeI	TCCRAC	2 cut(s)	256, 1768
Mox20I	TGGCCA	1 cut(s)	227
Mph1103I	ATGCAT	1 cut(s)	2275
MroI	TCCGGA	1 cut(s)	1084
MroXI	GAANNNNTTC	4 cut(s)	269, 416, 1355, 1944
MscI	TGGCCA	1 cut(s)	227
MseI	TTAA	9 cut(s)	200, 242, 702, 774, 843, 1170, 1328, 1554, 2079
MslI	CAYNNNNRTG	2 cut(s)	711, 1604
Msp20I	TGGCCA	1 cut(s)	227
MspA1I	CMGCKG	3 cut(s)	570, 2047, 2072
MspR9I	CCNGG	8 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006, 2436
MunI	CAATTG	1 cut(s)	1394
Mva1269I	GAATGC	1 cut(s)	1693
MvaI	CCWGG	1 cut(s)	2436
MvnI	CGCG	2 cut(s)	684, 686
MwoI	GCNNNNNNNGC	2 cut(s)	613, 1813
NciI	CCSGG	7 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006
NdeI	CATATG	1 cut(s)	2337
NdeII	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
NlaIII	CATG	8 cut(s)	81, 625, 1417, 1609, 1825, 2134, 2173, 2443
NlaIV	GGNNCC	6 cut(s)	10, 38, 39, 1249, 1315, 1658
NmuCI	GTSAC	2 cut(s)	80, 335
NsiI	ATGCAT	1 cut(s)	2275
NspI	RCATGY	2 cut(s)	2134, 2173
PaeR7I	CTCGAG	2 cut(s)	1014, 1764
PaqCI	CACCTGC	1 cut(s)	2315
PciI	ACATGT	1 cut(s)	2130
PciSI	GCTCTTC	1 cut(s)	2383
PcsI	WCGNNNNNNNCGW	2 cut(s)	552, 975
PctI	GAATGC	1 cut(s)	1693
PdmI	GAANNNNTTC	4 cut(s)	269, 416, 1355, 1944
PfeI	GAWTC	9 cut(s)	451, 529, 649, 1009, 1051, 1081, 1164, 1279, 1356
PflMI	CCANNNNNTGG	1 cut(s)	2024
PfoI	TCCNGGA	2 cut(s)	286, 1274
PkrI	GCNGC	6 cut(s)	609, 1742, 1892, 2049, 2118, 2121
PleI	GAGTC	6 cut(s)	216, 610, 756, 954, 1785, 2291
PpsI	GAGTC	6 cut(s)	216, 610, 756, 954, 1785, 2291
PpuMI	RGGWCCY	1 cut(s)	37
PscI	ACATGT	1 cut(s)	2130
Psp124BI	GAGCTC	4 cut(s)	372, 816, 1765, 1804
Psp5II	RGGWCCY	1 cut(s)	37
Psp6I	CCWGG	1 cut(s)	2434
PspEI	GGTNACC	1 cut(s)	989
PspFI	CCCAGC	3 cut(s)	509, 1660, 2122
PspGI	CCWGG	1 cut(s)	2434
PspN4I	GGNNCC	6 cut(s)	10, 38, 39, 1249, 1315, 1658
PspPI	GGNCC	7 cut(s)	8, 37, 353, 472, 860, 1076, 1580
PspPPI	RGGWCCY	1 cut(s)	37
PsrI	GAACNNNNNNTAC	2 cut(s)	839, 871
PstI	CTGCAG	1 cut(s)	2052
PsuI	RGATCY	4 cut(s)	763, 1380, 1627, 1656
PvuII	CAGCTG	2 cut(s)	2047, 2072
RsaI	GTAC	6 cut(s)	545, 1371, 1920, 2129, 2162, 2168
RsaNI	GTAC	6 cut(s)	544, 1370, 1919, 2128, 2161, 2167
RseI	CAYNNNNRTG	2 cut(s)	711, 1604
SacI	GAGCTC	4 cut(s)	372, 816, 1765, 1804
SapI	GCTCTTC	1 cut(s)	2383
SaqAI	TTAA	9 cut(s)	200, 242, 702, 774, 843, 1170, 1328, 1554, 2079
SatI	GCNGC	6 cut(s)	608, 1741, 1891, 2048, 2117, 2120
Sau3AI	GATC	8 cut(s)	387, 763, 883, 1188, 1380, 1627, 1656, 2056
Sau96I	GGNCC	7 cut(s)	8, 37, 353, 472, 860, 1076, 1580
SchI	GAGTC	6 cut(s)	217, 611, 757, 955, 1785, 2291
ScrFI	CCNGG	8 cut(s)	217, 218, 288, 1003, 1276, 1363, 2006, 2436
SduI	GDGCHC	5 cut(s)	372, 816, 1421, 1765, 1804
SfaNI	GCATC	1 cut(s)	2282
SfcI	CTRYAG	1 cut(s)	2048
Sfr274I	CTCGAG	2 cut(s)	1014, 1764
SinI	GGWCC	3 cut(s)	37, 353, 1580
SlaI	CTCGAG	2 cut(s)	1014, 1764
SmaI	CCCGGG	1 cut(s)	218
SmiMI	CAYNNNNRTG	2 cut(s)	711, 1604
SmlI	CTYRAG	6 cut(s)	317, 533, 815, 1014, 1319, 1764
SmoI	CTYRAG	6 cut(s)	317, 533, 815, 1014, 1319, 1764
SsiI	CCGC	8 cut(s)	146, 408, 495, 506, 570, 1724, 1890, 2116
SspI	AATATT	2 cut(s)	467, 1429
SspMI	CTAG	7 cut(s)	26, 662, 1343, 1481, 2201, 2222, 2301
SstI	GAGCTC	4 cut(s)	372, 816, 1765, 1804
StyD4I	CCNGG	8 cut(s)	215, 216, 286, 1001, 1274, 1361, 2004, 2434
TaaI	ACNGT	1 cut(s)	1909
TaiI	ACGT	4 cut(s)	114, 549, 1478, 1947
TaqI	TCGA	7 cut(s)	969, 978, 1015, 1685, 1765, 2135, 2331
TatI	WGTACW	2 cut(s)	1918, 2166
TauI	GCSGC	2 cut(s)	1893, 2119
TfiI	GAWTC	9 cut(s)	451, 529, 649, 1009, 1051, 1081, 1164, 1279, 1356
Tru1I	TTAA	9 cut(s)	200, 242, 702, 774, 843, 1170, 1328, 1554, 2079
Tru9I	TTAA	9 cut(s)	200, 242, 702, 774, 843, 1170, 1328, 1554, 2079
TscAI	CASTG	4 cut(s)	355, 1801, 1958, 2196
TseFI	GTSAC	2 cut(s)	80, 335
TseI	GCWGC	4 cut(s)	607, 1740, 2047, 2119
Tsp45I	GTSAC	2 cut(s)	80, 335
TspDTI	ATGAA	6 cut(s)	66, 865, 896, 1073, 1588, 1636
TspMI	CCCGGG	1 cut(s)	216
TspRI	CASTG	4 cut(s)	355, 1801, 1958, 2196
Van91I	CCANNNNNTGG	1 cut(s)	2024
VpaK11BI	GGWCC	3 cut(s)	37, 353, 1580
XapI	RAATTY	8 cut(s)	429, 576, 1260, 1324, 1440, 1595, 1621, 2149
XceI	RCATGY	2 cut(s)	2134, 2173
XhoI	CTCGAG	2 cut(s)	1014, 1764
XmaI	CCCGGG	1 cut(s)	216
XmiI	GTMKAC	3 cut(s)	2001, 2295, 2406
XmnI	GAANNNNTTC	4 cut(s)	269, 416, 1355, 1944
XspI	CTAG	7 cut(s)	26, 662, 1343, 1481, 2201, 2222, 2301
Zsp2I	ATGCAT	1 cut(s)	2275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.