Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

33103428 .. 33106505

Gene Structure 3078 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G022990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2919 bp

ATGGTACTGGTAGGTTCCCCTTTTTGGCATAATCTCCTCCACATTTCTTTCATTTTCCTTTTCCTTCCACAATGTTGTTTCTGCGTTGGTGACCTTGACGCACTGTTGATGCTCAAGAAAGCCATGATTGGCCCCAAAGGTTCAGGGCTAGAGAACTGGGACCCCTCTTCTCATTACTACTGCTCCTTCTCTGGAGTTTCATGTGACCACCAATCACGAGTTGTTGCTCTCAACGTGTCCGGTTTGTCTCTGTTTGGCTCGCTTCCGGCGGAGATTCGGCTTCTGAACAAGCTCGTCAACCTCACCATTGCCAACAACAACTTAACAGGGAGTCTTCCCCGGGAGATGGCCAACCTGACTTGTTTAAAGCTCTTGAACATCTCCAACAACTTGTTCGTTGGAAGATTTCCCGGAGAAATCACGCTCAGAATGACAGAGCTTGAGGTTCTCGACGCCTTTGACAACAACTTCACTGGTCCACTTCCGGTAGAGCTCGTCAAACTCAAAAAGATCAAACATCTAAATCTTGGCGGGAACTTCTTCGCCGGAGAAATTCCCGAAAGTTATTCAAAGATTCAAAGCTTAGAATATTTGGGCCTGCACGGTAACTCGCTTGGTGGAAAGTTTCCGCCCAGCTTGGCTCTGTTGCAGAATCTCAAGGAATTGTACGTTGGTTACGACAACACTTACAGCGGTGGAATTCCACCAGAGTTGGGAACGATGGAGTCGCTGCAAGTGCTTGACATGGCCAGCTGTAACCTCACCGGCACGATTCCTGTCAGTCTAGGCTTTTTGAAGAACTTACGCTCGTTGTTTCTACATATTAACCATTTGAGTGGTTATATACCTCCAAAACTTTCAGGTATGGTGAGTCTGATGTCTTTGGATCTCTCTATTAACGAACTCACCGGAGAAATACCAGAAAGTCTCTCGGAGCTCAAGAACCTTACCCTACTCAACCTGTATAAGAACAGTTTTTACGGCCCGATACGCAACTTCATCGGCGATCTTCCTCATCTGGAGGTGCTTCAAGTTTGGGGGAACAACTTTACATTTGAACTACCTGAGAGTCTTGGGCGGAATGGAAGGCTCAAAGACCTCGATGTAACCGGTAACCACCTTACCGGGTTGATTCCTCGAGATTTGTGCAAAGGAGGGAGGTTAAGGTCGTTGGTTCTGATGGATAATCACTTCTTTGGGCCGATTCCGGATGAATTGGGTCGGTGCAAGTCGCTCGGGCATATCCTATTGAAGAAAAACACACTCAACGGGACTATTCCTGCCGGAATCTTTAATAACTTACCTGATTTGATCAGGATTCAGCTGAATGATAACTACTTGTCTGGAAAACTTCCATTGCAGATGTCGGGTGCCTCACTCGAAATTCTCACACTTTCCCGGAATCAGATTTCTGGAAAAATCCCATCCACCATTGGAACCCTCAAGAATTTAAAACATCTTTCGCTAGATGGGAACAGATTCTCCGGGCAAGTACCAACAGAGATCTTCAGTTTACAATTGCTAAGTAAACTCAACATGAGCACCAACAATATTGGTGGTGAAATTCCGGCTTCAATCTCTGGTTGTTCCTCTCTGACGTTCCTAGATTTTAGTCAAAACACTTTTGTTGGGGAAATCCCAAGAGGGATAAGTAAGCTGAAAGTAATTCAGTTTCTTAATTTTTCAAGGAACAAGCTAACAGGTCCAATACCTGTGGAATTCATATACATGGGGAGCCTCACAAATTTGGATCTCTCCTATAACAATTTCATTGGTAGGATCCCAGCCGGAGGTCAGATTTTATGGTCGAATGCTTGGTTTGAAGGAAATCCCAATCTGTGTTCGGCGGAAAAGGTGTTGTGCAGCTCTGATAACCACCCACGAGCTCGAGACCATAAAGTCATACTCACTGGAGCTCTGCTAACTTTGCTACTCATGTTGCTTCTTATATATGTAGTTATCAGGGTTTATAGGAAGAGGAAGACCACAAAGATAGAGAAATGTGGTTCTTGGAAGCTAACAGTTTTCCAAGTACATCTCAATCTCAACATAGAAGACGTTCTCCAGTGTCTAAAAGCAGAGAACATTATCGGCAGGGGTGGTGCTGGAATTGTCTACCGGGGGACAATGCCAAATGGTGGTGATATGGCAATCAAACAGCTGCAGAGGGATCAGGGATTTTCAGCTGAAATTAAAACATTGGGACAAATCAAGCACCGAAATATAGTGCGGCTGCTGGGGTACATGTCGAACAAGGACACGAATTTGTTGCTGTACGAGTACATGCCTAATGGTAGCTTGGGACAACTTTTGCGTGGGCCTAATGGAGGGCATTTGGGGTGGGAAATCAGGTACAAGATTGCTGTGGAAGCTGCCAAGGGATTGTGTTATCTCCACCATGACTGTTCGCCTCTGATTATACACAGGGATATAAAGTCCCATAACATCTTGCTGGACTCTAACTTTGAAGCTCATGTTGCTGACTTTGGGCTTGCCAAGTGCTTTCAAGGACCAACAGATTGCATGTCTTCGCTTGCTGGTACCTTTGGTTACATTGCCCCAGAGTACGGCTGCACACTACAAGTGGATGAGAAAATCGATGTGTACAGCTTTGGTGTGGTTCTGCTGGAACTAGTAACAGGGAGGAAACCAGTGGTGGAACTAGTCGAAGAAGCTGTGAACTTAGTAACTTGGGTAAGGAAAACCACATCAGAAATCCCTCAGTCATCCAATGCATCATCTGTATTTGCAGTTGTGGACTCTACGCTCAGTGAGTTCCCATTGGCAGGTGTCGAGCATATGTTCAAGATAGCGATGATGTGCGTCAAGAATCACAGCTCTTCAAGACCTACAATGAGGGAAGTAGTCTACTTGCTCGCTAATCCTCCTCCTTCTGCTCCAGGCATGACAAACCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

972

Amino Acids

107.03

Weight (kDa)

8.34

Isoelectric Point (pI)

35.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	31 - 69	1.3e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	165 - 277	9.2e-10	Leucine-rich repeat region
LRR_14	PF23598	460 - 564	1.7e-09	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	692 - 956	4.5e-38	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	693 - 956	5.1e-42	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2777
AasI	GACNNNNNNGTC	1 cut(s)	1898
Acc36I	ACCTGC	1 cut(s)	2777
Acc65I	GGTACC	1 cut(s)	2540
AccB1I	GGYRCC	2 cut(s)	1370, 2540
AccB7I	CCANNNNNTGG	1 cut(s)	2138
AccI	GTMKAC	2 cut(s)	2115, 2868
AccIII	TCCGGA	1 cut(s)	1207
AciI	CCGC	7 cut(s)	269, 531, 629, 693, 1078, 1847, 2230
AclWI	GGATC	5 cut(s)	894, 1758, 1774, 1787, 2178
AcoI	YGGCCR	2 cut(s)	348, 747
AcsI	RAATTY	8 cut(s)	552, 699, 1383, 1447, 1563, 1718, 1744, 2263
AcuI	CTGAAG	1 cut(s)	1492
AcyI	GRCGYC	1 cut(s)	453
AfiI	CCNNNNNNNGG	8 cut(s)	24, 346, 713, 1790, 2138, 2327, 2567, 2786
AflIII	ACRYGT	2 cut(s)	234, 2244
AgeI	ACCGGT	1 cut(s)	1109
AhlI	ACTAGT	2 cut(s)	2632, 2662
AjnI	CCWGG	1 cut(s)	2899
AjuI	GAANNNNNNNTTGG	4 cut(s)	377, 409, 654, 686
AloI	GAACNNNNNNTCC	2 cut(s)	1466, 1498
Alw21I	GWGCWC	5 cut(s)	495, 939, 1544, 1888, 1918
Alw26I	GTCTC	3 cut(s)	252, 932, 1884
AlwI	GGATC	5 cut(s)	894, 1758, 1774, 1787, 2178
Ama87I	CYCGRG	4 cut(s)	339, 1137, 1235, 1887
Aor13HI	TCCGGA	1 cut(s)	1207
AoxI	GGCC	7 cut(s)	130, 348, 595, 747, 982, 1199, 2318
ApeKI	GCWGC	6 cut(s)	730, 1863, 2161, 2233, 2372, 2571
ApoI	RAATTY	8 cut(s)	552, 699, 1383, 1447, 1563, 1718, 1744, 2263
ArsI	GACNNNNNNTTYG	2 cut(s)	2447, 2479
AsiGI	ACCGGT	1 cut(s)	1109
Asp700I	GAANNNNTTC	3 cut(s)	539, 1478, 2058
Asp718I	GGTACC	1 cut(s)	2540
AspS9I	GGNCC	9 cut(s)	131, 160, 476, 595, 983, 1199, 1703, 2318, 2510
AsuC2I	CCSGG	7 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120
AsuHPI	GGTGA	7 cut(s)	101, 295, 754, 880, 898, 1571, 2153
AvaI	CYCGRG	4 cut(s)	339, 1137, 1235, 1887
AvaII	GGWCC	4 cut(s)	160, 476, 1703, 2510
BalI	TGGCCA	2 cut(s)	350, 749
BamHI	GGATCC	1 cut(s)	1779
BanI	GGYRCC	2 cut(s)	1370, 2540
BanII	GRGCYC	4 cut(s)	495, 939, 1888, 1918
BauI	CACGAG	2 cut(s)	216, 1881
BbsI	GAAGAC	4 cut(s)	326, 1988, 2061, 2520
Bbv12I	GWGCWC	5 cut(s)	495, 939, 1544, 1888, 1918
BbvI	GCAGC	6 cut(s)	717, 1875, 2148, 2220, 2359, 2558
BccI	CCATC	5 cut(s)	340, 715, 1174, 1432, 1463
BceAI	ACGGC	2 cut(s)	997, 2584
BcgI	CGANNNNNNTGC	8 cut(s)	982, 1016, 1128, 1162, 1216, 1250, 2251, 2285
BciT130I	CCWGG	1 cut(s)	2901
BclI	TGATCA	1 cut(s)	1311
BcnI	CCSGG	7 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120
BcoDI	GTCTC	3 cut(s)	252, 932, 1884
BcuI	ACTAGT	2 cut(s)	2632, 2662
BfaI	CTAG	6 cut(s)	149, 785, 1466, 1604, 2633, 2663
BfmI	CTRYAG	1 cut(s)	2162
BfuAI	ACCTGC	1 cut(s)	2777
BglII	AGATCT	1 cut(s)	1503
BisI	GCNGC	7 cut(s)	731, 1864, 2162, 2231, 2234, 2373, 2572
BlsI	GCNGC	7 cut(s)	732, 1865, 2163, 2232, 2235, 2374, 2573
Bme1390I	CCNGG	8 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120, 2901
Bme18I	GGWCC	4 cut(s)	160, 476, 1703, 2510
BmeT110I	CYCGRG	4 cut(s)	339, 1137, 1235, 1887
BmgT120I	GGNCC	9 cut(s)	131, 160, 476, 595, 983, 1199, 1703, 2318, 2510
BmiI	GGNNCC	9 cut(s)	16, 133, 161, 162, 1372, 1438, 1736, 1781, 2542
BmrFI	CCNGG	8 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120, 2901
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	2 cut(s)	99, 2744
BmuI	ACTGGG	1 cut(s)	166
BpiI	GAAGAC	4 cut(s)	326, 1988, 2061, 2520
BpmI	CTGGAG	5 cut(s)	213, 1040, 1932, 2048, 2883
BpuEI	CTTGAG	5 cut(s)	98, 461, 641, 923, 1427
BpuMI	CCSGG	7 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120
Bsa29I	ATCGAT	1 cut(s)	2598
BsaHI	GRCGYC	1 cut(s)	453
BsaI	GGTCTC	1 cut(s)	1884
BsaJI	CCNNGG	4 cut(s)	338, 339, 2119, 2376
BsaWI	WCCGGW	5 cut(s)	239, 484, 908, 1109, 1207
BsaXI	ACNNNNNCTCC	4 cut(s)	167, 197, 1466, 1496
Bsc4I	CCNNNNNNNGG	8 cut(s)	24, 346, 713, 1790, 2138, 2327, 2567, 2786
Bse118I	RCCGGY	2 cut(s)	764, 1109
Bse1I	ACTGG	6 cut(s)	12, 161, 478, 1915, 2065, 2651
Bse3DI	GCAATG	3 cut(s)	306, 1355, 2553
BseAI	TCCGGA	1 cut(s)	1207
BseBI	CCWGG	1 cut(s)	2901
BseCI	ATCGAT	1 cut(s)	2598
BseDI	CCNNGG	4 cut(s)	338, 339, 2119, 2376
BseGI	GGATG	4 cut(s)	1216, 1424, 2593, 2726
BseLI	CCNNNNNNNGG	8 cut(s)	24, 346, 713, 1790, 2138, 2327, 2567, 2786
BseMI	GCAATG	3 cut(s)	306, 1355, 2553
BseMII	CTCAG	4 cut(s)	439, 1056, 2735, 2782
BseNI	ACTGG	6 cut(s)	12, 161, 478, 1915, 2065, 2651
BseRI	GAGGAG	2 cut(s)	26, 2877
BseXI	GCAGC	6 cut(s)	717, 1875, 2148, 2220, 2359, 2558
BseYI	CCCAGC	3 cut(s)	632, 1783, 2236
BsgI	GTGCAG	3 cut(s)	584, 1882, 2557
BshFI	GGCC	7 cut(s)	132, 350, 597, 749, 984, 1201, 2320
BshNI	GGYRCC	2 cut(s)	1370, 2540
BshTI	ACCGGT	1 cut(s)	1109
BshVI	ATCGAT	1 cut(s)	2598
BsiHKAI	GWGCWC	5 cut(s)	495, 939, 1544, 1888, 1918
BsiHKCI	CYCGRG	4 cut(s)	339, 1137, 1235, 1887
BslFI	GGGAC	6 cut(s)	173, 1285, 2137, 2217, 2316, 2422
BslI	CCNNNNNNNGG	8 cut(s)	24, 346, 713, 1790, 2138, 2327, 2567, 2786
BsmAI	GTCTC	3 cut(s)	252, 932, 1884
BsmFI	GGGAC	6 cut(s)	173, 1285, 2137, 2217, 2316, 2422
BsmI	GAATGC	1 cut(s)	1816
BsnI	GGCC	7 cut(s)	132, 350, 597, 749, 984, 1201, 2320
Bso31I	GGTCTC	1 cut(s)	1884
BsoBI	CYCGRG	4 cut(s)	339, 1137, 1235, 1887
Bsp1286I	GDGCHC	5 cut(s)	495, 939, 1544, 1888, 1918
Bsp13I	TCCGGA	1 cut(s)	1207
Bsp1407I	TGTACA	1 cut(s)	2604
Bsp143I	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
BspACI	CCGC	7 cut(s)	269, 531, 629, 693, 1078, 1847, 2230
BspANI	GGCC	7 cut(s)	132, 350, 597, 749, 984, 1201, 2320
BspCNI	CTCAG	4 cut(s)	438, 1057, 2734, 2781
BspDI	ATCGAT	1 cut(s)	2598
BspEI	TCCGGA	1 cut(s)	1207
BspLI	GGNNCC	9 cut(s)	16, 133, 161, 162, 1372, 1438, 1736, 1781, 2542
BspMAI	CTGCAG	1 cut(s)	2166
BspMI	ACCTGC	1 cut(s)	2777
BspPI	GGATC	5 cut(s)	894, 1758, 1774, 1787, 2178
BspQI	GCTCTTC	1 cut(s)	2845
BspT107I	GGYRCC	2 cut(s)	1370, 2540
BspTNI	GGTCTC	1 cut(s)	1884
BsrDI	GCAATG	3 cut(s)	306, 1355, 2553
BsrFI	RCCGGY	2 cut(s)	764, 1109
BsrGI	TGTACA	1 cut(s)	2604
BsrI	ACTGG	6 cut(s)	12, 161, 478, 1915, 2065, 2651
BssAI	RCCGGY	2 cut(s)	764, 1109
BssECI	CCNNGG	4 cut(s)	338, 339, 2119, 2376
BssMI	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
BssNI	GRCGYC	1 cut(s)	453
BssSI	CACGAG	2 cut(s)	216, 1881
BssT1I	CCWWGG	1 cut(s)	2376
Bst2BI	CACGAG	2 cut(s)	216, 1881
Bst2UI	CCWGG	1 cut(s)	2901
Bst4CI	ACNGT	5 cut(s)	105, 605, 974, 2023, 2405
Bst6I	CTCTTC	3 cut(s)	172, 1970, 2845
BstACI	GRCGYC	1 cut(s)	453
BstAUI	TGTACA	1 cut(s)	2604
BstC8I	GCNNGC	6 cut(s)	260, 599, 751, 2493, 2535, 2877
BstDEI	CTNAG	8 cut(s)	425, 583, 1065, 1523, 2683, 2721, 2768, 2916
BstEII	GGTNACC	2 cut(s)	89, 1112
BstENI	CCTNNNNNAGG	1 cut(s)	2325
BstF5I	GGATG	4 cut(s)	1216, 1424, 2593, 2726
BstKTI	GATC	8 cut(s)	513, 889, 1009, 1314, 1506, 1753, 1782, 2173
BstMAI	GTCTC	3 cut(s)	252, 932, 1884
BstMBI	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
BstMWI	GCNNNNNNNGC	5 cut(s)	736, 990, 1927, 2369, 2477
BstNI	CCWGG	1 cut(s)	2901
BstNSI	RCATGY	3 cut(s)	2248, 2287, 2527
BstPI	GGTNACC	2 cut(s)	89, 1112
BstSCI	CCNGG	8 cut(s)	338, 339, 409, 1124, 1397, 1484, 2118, 2899
BstSFI	CTRYAG	1 cut(s)	2162
BstV1I	GCAGC	6 cut(s)	717, 1875, 2148, 2220, 2359, 2558
BstV2I	GAAGAC	4 cut(s)	326, 1988, 2061, 2520
BstX2I	RGATCY	4 cut(s)	886, 1503, 1750, 1779
BstXI	CCANNNNNNTGG	1 cut(s)	836
BstYI	RGATCY	4 cut(s)	886, 1503, 1750, 1779
Bsu15I	ATCGAT	1 cut(s)	2598
BsuRI	GGCC	7 cut(s)	132, 350, 597, 749, 984, 1201, 2320
BsuTUI	ATCGAT	1 cut(s)	2598
BtgZI	GCGATG	1 cut(s)	2828
BtsCI	GGATG	4 cut(s)	1216, 1424, 2593, 2726
BtsIMutI	CAGTG	6 cut(s)	101, 471, 1908, 2072, 2658, 2776
BveI	ACCTGC	1 cut(s)	2777
Cac8I	GCNNGC	6 cut(s)	260, 599, 751, 2493, 2535, 2877
Cfr10I	RCCGGY	2 cut(s)	764, 1109
Cfr13I	GGNCC	9 cut(s)	131, 160, 476, 595, 983, 1199, 1703, 2318, 2510
Cfr9I	CCCGGG	1 cut(s)	339
ClaI	ATCGAT	1 cut(s)	2598
CseI	GACGC	3 cut(s)	107, 461, 2812
CspAI	ACCGGT	1 cut(s)	1109
CspCI	CAANNNNNGTGG	6 cut(s)	693, 728, 1537, 1572, 1695, 1730
DdeI	CTNAG	8 cut(s)	425, 583, 1065, 1523, 2683, 2721, 2768, 2916
DpnI	GATC	8 cut(s)	512, 888, 1008, 1313, 1505, 1752, 1781, 2172
DpnII	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
DraI	TTTAAA	2 cut(s)	366, 1452
DrdI	GACNNNNNNGTC	1 cut(s)	1898
DseDI	GACNNNNNNGTC	1 cut(s)	1898
EaeI	YGGCCR	2 cut(s)	348, 747
Eam1104I	CTCTTC	3 cut(s)	172, 1970, 2845
EarI	CTCTTC	3 cut(s)	172, 1970, 2845
EciI	GGCGGA	4 cut(s)	284, 618, 1093, 1862
Ecl136II	GAGCTC	4 cut(s)	493, 937, 1886, 1916
Eco130I	CCWWGG	1 cut(s)	2376
Eco24I	GRGCYC	4 cut(s)	495, 939, 1888, 1918
Eco31I	GGTCTC	1 cut(s)	1884
Eco47I	GGWCC	4 cut(s)	160, 476, 1703, 2510
Eco53kI	GAGCTC	4 cut(s)	493, 937, 1886, 1916
Eco57I	CTGAAG	1 cut(s)	1492
Eco88I	CYCGRG	4 cut(s)	339, 1137, 1235, 1887
Eco91I	GGTNACC	2 cut(s)	89, 1112
EcoICRI	GAGCTC	4 cut(s)	493, 937, 1886, 1916
EcoNI	CCTNNNNNAGG	1 cut(s)	2325
EcoO109I	RGGNCCY	1 cut(s)	160
EcoO65I	GGTNACC	2 cut(s)	89, 1112
EcoRI	GAATTC	2 cut(s)	699, 1718
EcoRII	CCWGG	1 cut(s)	2899
EcoT14I	CCWWGG	1 cut(s)	2376
EcoT22I	ATGCAT	1 cut(s)	2737
EcoT38I	GRGCYC	4 cut(s)	495, 939, 1888, 1918
ErhI	CCWWGG	1 cut(s)	2376
FaqI	GGGAC	6 cut(s)	173, 1285, 2137, 2217, 2316, 2422
FauI	CCCGC	1 cut(s)	524
FauNDI	CATATG	1 cut(s)	2799
FbaI	TGATCA	1 cut(s)	1311
FblI	GTMKAC	2 cut(s)	2115, 2868
Fnu4HI	GCNGC	7 cut(s)	731, 1864, 2162, 2231, 2234, 2373, 2572
FokI	GGATG	4 cut(s)	1223, 1411, 2600, 2713
FriOI	GRGCYC	4 cut(s)	495, 939, 1888, 1918
Fsp4HI	GCNGC	7 cut(s)	731, 1864, 2162, 2231, 2234, 2373, 2572
FspBI	CTAG	6 cut(s)	149, 785, 1466, 1604, 2633, 2663
GluI	GCNGC	7 cut(s)	731, 1864, 2162, 2231, 2234, 2373, 2572
GsaI	CCCAGC	3 cut(s)	636, 1787, 2240
GsuI	CTGGAG	5 cut(s)	213, 1040, 1932, 2048, 2883
HaeIII	GGCC	7 cut(s)	132, 350, 597, 749, 984, 1201, 2320
HgaI	GACGC	3 cut(s)	107, 461, 2812
Hin1I	GRCGYC	1 cut(s)	453
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HindIII	AAGCTT	1 cut(s)	580
HphI	GGTGA	7 cut(s)	101, 295, 754, 880, 898, 1571, 2153
Hpy166II	GTNNAC	9 cut(s)	298, 479, 1514, 1529, 2116, 2605, 2680, 2758, 2869
Hpy8I	GTNNAC	9 cut(s)	298, 479, 1514, 1529, 2116, 2605, 2680, 2758, 2869
Hpy99I	CGWCG	1 cut(s)	455
HpyAV	CCTTC	5 cut(s)	74, 196, 1080, 1817, 2901
HpyCH4III	ACNGT	5 cut(s)	105, 605, 974, 2023, 2405
HpyCH4IV	ACGT	4 cut(s)	234, 669, 1598, 2058
HpyF10VI	GCNNNNNNNGC	5 cut(s)	736, 990, 1927, 2369, 2477
HpyF3I	CTNAG	8 cut(s)	425, 583, 1065, 1523, 2683, 2721, 2768, 2916
HpySE526I	ACGT	4 cut(s)	234, 669, 1598, 2058
Hsp92I	GRCGYC	1 cut(s)	453
KflI	GGGWCCC	1 cut(s)	160
Kpn2I	TCCGGA	1 cut(s)	1207
KpnI	GGTACC	1 cut(s)	2544
Ksp22I	TGATCA	1 cut(s)	1311
Kzo9I	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
LguI	GCTCTTC	1 cut(s)	2845
LmnI	GCTCC	5 cut(s)	188, 934, 1734, 1913, 2902
Lsp1109I	GCAGC	6 cut(s)	717, 1875, 2148, 2220, 2359, 2558
LweI	GCATC	2 cut(s)	99, 2744
MaeI	CTAG	6 cut(s)	149, 785, 1466, 1604, 2633, 2663
MaeII	ACGT	4 cut(s)	234, 669, 1598, 2058
MalI	GATC	8 cut(s)	512, 888, 1008, 1313, 1505, 1752, 1781, 2172
MboI	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
MfeI	CAATTG	1 cut(s)	1517
MflI	RGATCY	4 cut(s)	886, 1503, 1750, 1779
MhlI	GDGCHC	5 cut(s)	495, 939, 1544, 1888, 1918
MlsI	TGGCCA	2 cut(s)	350, 749
MluNI	TGGCCA	2 cut(s)	350, 749
MlyI	GAGTC	6 cut(s)	340, 734, 880, 1078, 2450, 2753
MmeI	TCCRAC	2 cut(s)	379, 408
Mox20I	TGGCCA	2 cut(s)	350, 749
Mph1103I	ATGCAT	1 cut(s)	2737
MroI	TCCGGA	1 cut(s)	1207
MroXI	GAANNNNTTC	3 cut(s)	539, 1478, 2058
MscI	TGGCCA	2 cut(s)	350, 749
MseI	TTAA	9 cut(s)	323, 365, 825, 897, 1163, 1293, 1451, 1677, 2193
MslI	CAYNNNNRTG	2 cut(s)	834, 1727
Msp20I	TGGCCA	2 cut(s)	350, 749
MspA1I	CMGCKG	5 cut(s)	693, 753, 1324, 2161, 2186
MspR9I	CCNGG	8 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120, 2901
MunI	CAATTG	1 cut(s)	1517
Mva1269I	GAATGC	1 cut(s)	1816
MvaI	CCWGG	1 cut(s)	2901
MwoI	GCNNNNNNNGC	5 cut(s)	736, 990, 1927, 2369, 2477
NciI	CCSGG	7 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120
NdeI	CATATG	1 cut(s)	2799
NdeII	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
NlaIV	GGNNCC	9 cut(s)	16, 133, 161, 162, 1372, 1438, 1736, 1781, 2542
NmuCI	GTSAC	2 cut(s)	89, 203
NsiI	ATGCAT	1 cut(s)	2737
NspI	RCATGY	3 cut(s)	2248, 2287, 2527
PaeR7I	CTCGAG	2 cut(s)	1137, 1887
PaqCI	CACCTGC	1 cut(s)	2777
PciI	ACATGT	1 cut(s)	2244
PciSI	GCTCTTC	1 cut(s)	2845
PcsI	WCGNNNNNNNCGW	1 cut(s)	675
PctI	GAATGC	1 cut(s)	1816
PdmI	GAANNNNTTC	3 cut(s)	539, 1478, 2058
PflMI	CCANNNNNTGG	1 cut(s)	2138
PfoI	TCCNGGA	2 cut(s)	409, 1397
PinAI	ACCGGT	1 cut(s)	1109
PkrI	GCNGC	7 cut(s)	732, 1865, 2163, 2232, 2235, 2374, 2573
PleI	GAGTC	6 cut(s)	339, 733, 879, 1077, 2450, 2753
PpsI	GAGTC	6 cut(s)	339, 733, 879, 1077, 2450, 2753
PpuMI	RGGWCCY	1 cut(s)	160
PscI	ACATGT	1 cut(s)	2244
Psp124BI	GAGCTC	4 cut(s)	495, 939, 1888, 1918
Psp5II	RGGWCCY	1 cut(s)	160
Psp6I	CCWGG	1 cut(s)	2899
PspEI	GGTNACC	2 cut(s)	89, 1112
PspFI	CCCAGC	3 cut(s)	632, 1783, 2236
PspGI	CCWGG	1 cut(s)	2899
PspN4I	GGNNCC	9 cut(s)	16, 133, 161, 162, 1372, 1438, 1736, 1781, 2542
PspPI	GGNCC	9 cut(s)	131, 160, 476, 595, 983, 1199, 1703, 2318, 2510
PspPPI	RGGWCCY	1 cut(s)	160
PsrI	GAACNNNNNNTAC	2 cut(s)	962, 994
PstI	CTGCAG	1 cut(s)	2166
PsuI	RGATCY	4 cut(s)	886, 1503, 1750, 1779
PvuII	CAGCTG	4 cut(s)	753, 1324, 2161, 2186
RseI	CAYNNNNRTG	2 cut(s)	834, 1727
SacI	GAGCTC	4 cut(s)	495, 939, 1888, 1918
SapI	GCTCTTC	1 cut(s)	2845
SaqAI	TTAA	9 cut(s)	323, 365, 825, 897, 1163, 1293, 1451, 1677, 2193
SatI	GCNGC	7 cut(s)	731, 1864, 2162, 2231, 2234, 2373, 2572
Sau3AI	GATC	8 cut(s)	510, 886, 1006, 1311, 1503, 1750, 1779, 2170
Sau96I	GGNCC	9 cut(s)	131, 160, 476, 595, 983, 1199, 1703, 2318, 2510
SchI	GAGTC	6 cut(s)	340, 734, 880, 1078, 2450, 2753
ScrFI	CCNGG	8 cut(s)	340, 341, 411, 1126, 1399, 1486, 2120, 2901
SduI	GDGCHC	5 cut(s)	495, 939, 1544, 1888, 1918
SfaNI	GCATC	2 cut(s)	99, 2744
SfcI	CTRYAG	1 cut(s)	2162
Sfr274I	CTCGAG	2 cut(s)	1137, 1887
SinI	GGWCC	4 cut(s)	160, 476, 1703, 2510
SlaI	CTCGAG	2 cut(s)	1137, 1887
SmaI	CCCGGG	1 cut(s)	341
SmiMI	CAYNNNNRTG	2 cut(s)	834, 1727
SmlI	CTYRAG	7 cut(s)	113, 440, 656, 938, 1137, 1442, 1887
SmoI	CTYRAG	7 cut(s)	113, 440, 656, 938, 1137, 1442, 1887
SpeI	ACTAGT	2 cut(s)	2632, 2662
SsiI	CCGC	7 cut(s)	269, 531, 629, 693, 1078, 1847, 2230
SspI	AATATT	2 cut(s)	590, 1552
SspMI	CTAG	6 cut(s)	149, 785, 1466, 1604, 2633, 2663
SstI	GAGCTC	4 cut(s)	495, 939, 1888, 1918
StyD4I	CCNGG	8 cut(s)	338, 339, 409, 1124, 1397, 1484, 2118, 2899
StyI	CCWWGG	1 cut(s)	2376
TaaI	ACNGT	5 cut(s)	105, 605, 974, 2023, 2405
TaiI	ACGT	4 cut(s)	237, 672, 1601, 2061
TatI	WGTACW	3 cut(s)	2032, 2280, 2604
TauI	GCSGC	1 cut(s)	2233
Tru1I	TTAA	9 cut(s)	323, 365, 825, 897, 1163, 1293, 1451, 1677, 2193
Tru9I	TTAA	9 cut(s)	323, 365, 825, 897, 1163, 1293, 1451, 1677, 2193
TscAI	CASTG	6 cut(s)	108, 478, 1915, 2072, 2658, 2776
TseFI	GTSAC	2 cut(s)	89, 203
TseI	GCWGC	6 cut(s)	730, 1863, 2161, 2233, 2372, 2571
Tsp45I	GTSAC	2 cut(s)	89, 203
TspDTI	ATGAA	6 cut(s)	40, 189, 988, 1227, 1711, 1759
TspMI	CCCGGG	1 cut(s)	339
TspRI	CASTG	6 cut(s)	108, 478, 1915, 2072, 2658, 2776
Van91I	CCANNNNNTGG	1 cut(s)	2138
VpaK11BI	GGWCC	4 cut(s)	160, 476, 1703, 2510
XagI	CCTNNNNNAGG	1 cut(s)	2325
XapI	RAATTY	8 cut(s)	552, 699, 1383, 1447, 1563, 1718, 1744, 2263
XceI	RCATGY	3 cut(s)	2248, 2287, 2527
XhoI	CTCGAG	2 cut(s)	1137, 1887
XmaI	CCCGGG	1 cut(s)	339
XmiI	GTMKAC	2 cut(s)	2115, 2868
XmnI	GAANNNNTTC	3 cut(s)	539, 1478, 2058
XspI	CTAG	6 cut(s)	149, 785, 1466, 1604, 2633, 2663
Zsp2I	ATGCAT	1 cut(s)	2737

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.