Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

33146960 .. 33150027

Gene Structure 3068 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G023000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGGTACTGGTAGGCTCCCGTTGTTGGCATAATCTGCTCCACATTTCTTTCATTTTCCTTTTCCTTCCACAATGTTGTTTCTGCGTTGGTGACCTTGACGCACTGTTGATGCTCAAGAAAGCCATGATTGGCCCCAAAGGTTCAGGGCTAGAGAACTGGGACCCCTCTTCTCATTACTACTGCTCCTTCTCTGGAGTTTCATGTGACCACCAATCACGAGTTGTTGCTCTCAACGTGTCCGGTTTGCCTCTGTTTGGGTCGATTCCAGCAGAGATTGGGCTTCTAAACAAACTCGTCAACCTCACCATTGCCGACAACAACTTCACAGGGAAGCTTCCCCTCGAGATGGCCAACCTGACTTGTTTAAAGCTCTTGAACATCTCCAACAACTTGTTCAATGATCTTGACGCCTATAATAACAACTATAATGGCCCACTTCCGGTAGAGCTCGTCAATCTCAAAAAGCTCAAATATCTGCATCTTGGCGGGAACTACTTCACTGGAGAAATTCCGAATAGTTATTCAGAGATTCAAAGCTTGGAGTATCTGGGCCTCAACGGTATCGGGCTCAACGGAATGTTCCCTTCCAGCTTGGCTCGGCTAAAGAATCTGAAAGAATTGTACGTTGGTTACTACAATACTTATAGTGGTGGAATTCCACCTGAGTTGGGAAGGATGGAGTCGTTGCAAGTGCTTGACATGGCCAGCTCCAACCTCACCGGCACGATTCCGGTCAGTCTAGGCTTTTTGAAGAACTTACGCCGGTTGTATCTACAGATGAACTGTTTGAGTGGTTATATACCTCCAAAACTTTCAGGTATGGTGAGCCTGATGTCTTTGGATCTCTCTATTAACGAACTCACCGGAGAAATACCAGAAAGTCTCTCGGAGCTCAAGAACCTTACCCTACTCAACCTGTATAAGAACAGTTTTTACGGCCCGATACGCAACTTCATCGGCGATCTTCCTCATCTGGAGGTGCTTCAAGTTTGGGGGAACAACTTTACATTTGAACTACCTGAGAGTCTTGGCCGGAATGGAAGGCTCAAAGACCTCGATGTAACCGGTAACCACCTCACCGGGTTGATTCCTCGAGATTTGTGCAAAGGAGGGAGGTTAAGGTCGTTGATTCTGATGGATAATCACTTCTTTGGGCCGATTCCGGAGGAATTGGGTCGGTGCAAGTCGCTCGGGCATATCCTATTGAAGAAAAACACACTCAACGGGACTATTCCTGCCGGAATCTTTAATAACTTACCTGATTTGATCAGGATTGAGCTGAATGATAACTACTTGTCTGGAAAACTTCCATTGCAGATGTCGGGTGCCTCACTCGAAATTCTCACACTTTCCCGGAATCAGATTTCTGGAAAAATCCCATCCACCATTGGAACCCTCAAGAATTTAAAACATCTTTCGCTAGATGTGAACAGATTCTCCGGGCAAGTACCAACAGAGATCTTCAGTTTACAATTGCTAAGTAAACTCAACATGAGCACCAACAATATTGGTGGTGAAATTCCGGCTTCAATCTCTGGTTGTTCCTCTCTGACGTTCCTAGATTTTAGTCAAAACACTTTTGTTGGGGAAATCCCAAGAGGGATAAGTAAGCTGAAAGTAATTCAGTTTCTTAATTTTTCAAGGAACAAGCTAACAGGTCCAATACCTGTGGAATTCATGTACATGGGGAGCCTCACAAATTTGGATCTCTCCTATAACAATTTCATTGGTAGGATCCCAGCCGGAGGTCAGTTTTTATGGTCGAATGCTTTGTTTGAAGGAAATCCCAATCTGTGTTCGGCGGAAAAGGTGTTGTGCAGCTCTGATAACCACCCACGAGCTCGAGACCATAAAGTCATCATCGTTGGACTCACTGGAGCTCTGCTAACTTTGCTACTCATGTTGCTTCTTATATATGTAGTTATCAGGGTTTATAGGAAGAGGAAGACCACAAAGATAGAGAAATGTGGTTCTTGGAAGCTAACAGTTTTCCAAGTACAGCTCAATCTCAACATAGAAGACGTTCTCCAGTGTCTAAAAGCAGAGAACATTATCGGCAGGGGTGGTGTTGTCTACCGGGGGACAATGCCAAATGGTGGTGATATTGCAATCAAACAGCTGCAGAGGGATCAAGGATTTTCAGCTGAAATTAAAACATTGGGACAAATCAAGCACCGAAATATAGTGCGGCTGCTGGGGTACATGTCGAGCAAGGACACGAATTTGTTGCTGTACGAGTACATGCCTAATGGTAGCTTGGGACAACTTTTGCGTGGGCCTAATGGAGGGCATTTGGGGTGGGAAATCAGGTACAAGATTGCTGTGGAAGCTGCCAAGGGATTGTGTTATCTCCACCATGACTGTTCGCCTCTGATTATACACAGGGATGTAAAGTCCCATAACATCTTGCTGGACTCTAACTTTGAAGCTCATGTTGCTGACTTTGGGCTTGCCAAGTGCTTTCAAGGACCAACAGATTGCATGTCTTCGCTTGCTGGTACCTTTGGTTACATTGCCCCAGAGTACGGTTGCACACTACAAGTGGATGAGAAAATCGATGTGTACAGCTTTGGTGTGGTTCTGCTGGAACTAGTAACAGGGAGGAAACCAGTAGTTGAACTAGTCGAAGAAGCTGTGAACCTAGTAACTTGGGTAAGGAAAACCACATCAGAAATCCCTCAGTCATCCAATGCATCATCTGTACTTGCAGTTGTGGACTCTAGGCTCAGTGAGTTCCCATTGTCAGGTGTCGAGCATATGTTCAAGATAGCGATGATGTGCGTCAAGAATCACAGCTCTTCAAGACCTACAATGAGGGAAGTAGTCTACTTGCTCACTAATCCTCCTCCTTCTGCTCCAGGCATGACAAACACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

958

Amino Acids

105.77

Weight (kDa)

8.47

Isoelectric Point (pI)

35.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	31 - 69	1.2e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	149 - 260	1e-11	Leucine-rich repeat region
LRR_14	PF23598	445 - 550	3.9e-09	Leucine-rich repeat region
Pkinase	PF00069	684 - 942	2.6e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	685 - 942	8.6e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1853
Acc65I	GGTACC	1 cut(s)	2498
AccB1I	GGYRCC	2 cut(s)	1325, 2498
AccB7I	CCANNNNNTGG	1 cut(s)	2096
AccI	GTMKAC	2 cut(s)	2073, 2826
AccIII	TCCGGA	1 cut(s)	1162
AciI	CCGC	3 cut(s)	486, 1802, 2188
AclWI	GGATC	5 cut(s)	849, 1713, 1729, 1742, 2136
AcoI	YGGCCR	3 cut(s)	348, 702, 1030
AcsI	RAATTY	8 cut(s)	507, 654, 1338, 1402, 1518, 1673, 1699, 2221
AcuI	CTGAAG	1 cut(s)	1447
AcyI	GRCGYC	1 cut(s)	408
AflIII	ACRYGT	2 cut(s)	234, 2202
AgeI	ACCGGT	1 cut(s)	1064
AhlI	ACTAGT	2 cut(s)	2590, 2620
AjnI	CCWGG	1 cut(s)	2857
AjuI	GAANNNNNNNTTGG	4 cut(s)	377, 409, 609, 641
AloI	GAACNNNNNNTCC	2 cut(s)	1421, 1453
Alw21I	GWGCWC	5 cut(s)	450, 894, 1499, 1843, 1882
Alw26I	GTCTC	2 cut(s)	887, 1839
AlwI	GGATC	5 cut(s)	849, 1713, 1729, 1742, 2136
Ama87I	CYCGRG	4 cut(s)	341, 1092, 1190, 1842
Aor13HI	TCCGGA	1 cut(s)	1162
AoxI	GGCC	9 cut(s)	130, 348, 430, 550, 702, 937, 1030, 1154, 2276
ApeKI	GCWGC	4 cut(s)	1818, 2119, 2191, 2330
ApoI	RAATTY	8 cut(s)	507, 654, 1338, 1402, 1518, 1673, 1699, 2221
ArsI	GACNNNNNNTTYG	2 cut(s)	2405, 2437
AsiGI	ACCGGT	1 cut(s)	1064
Asp700I	GAANNNNTTC	3 cut(s)	494, 1433, 2022
Asp718I	GGTACC	1 cut(s)	2498
AspS9I	GGNCC	9 cut(s)	131, 160, 431, 550, 938, 1154, 1658, 2276, 2468
AsuC2I	CCSGG	4 cut(s)	1081, 1354, 1441, 2078
AsuHPI	GGTGA	8 cut(s)	101, 295, 709, 835, 853, 1069, 1526, 2111
AvaI	CYCGRG	4 cut(s)	341, 1092, 1190, 1842
AvaII	GGWCC	3 cut(s)	160, 1658, 2468
BalI	TGGCCA	2 cut(s)	350, 704
BamHI	GGATCC	1 cut(s)	1734
BanI	GGYRCC	2 cut(s)	1325, 2498
BanII	GRGCYC	5 cut(s)	450, 570, 894, 1843, 1882
BauI	CACGAG	2 cut(s)	216, 1836
BbsI	GAAGAC	3 cut(s)	1952, 2025, 2478
Bbv12I	GWGCWC	5 cut(s)	450, 894, 1499, 1843, 1882
BbvI	GCAGC	4 cut(s)	1830, 2106, 2178, 2317
BccI	CCATC	4 cut(s)	340, 670, 1129, 1387
BceAI	ACGGC	1 cut(s)	952
BcgI	CGANNNNNNTGC	8 cut(s)	937, 971, 1083, 1117, 1171, 1205, 2209, 2243
BciT130I	CCWGG	1 cut(s)	2859
BclI	TGATCA	1 cut(s)	1266
BcnI	CCSGG	4 cut(s)	1081, 1354, 1441, 2078
BcoDI	GTCTC	2 cut(s)	887, 1839
BcuI	ACTAGT	2 cut(s)	2590, 2620
BfaI	CTAG	8 cut(s)	149, 740, 1421, 1559, 2591, 2621, 2642, 2721
BfmI	CTRYAG	2 cut(s)	773, 2120
BglII	AGATCT	1 cut(s)	1458
BisI	GCNGC	5 cut(s)	1819, 2120, 2189, 2192, 2331
BlsI	GCNGC	5 cut(s)	1820, 2121, 2190, 2193, 2332
Bme1390I	CCNGG	5 cut(s)	1081, 1354, 1441, 2078, 2859
Bme18I	GGWCC	3 cut(s)	160, 1658, 2468
BmeT110I	CYCGRG	4 cut(s)	341, 1092, 1190, 1842
BmgT120I	GGNCC	9 cut(s)	131, 160, 431, 550, 938, 1154, 1658, 2276, 2468
BmiI	GGNNCC	9 cut(s)	16, 133, 161, 162, 1327, 1393, 1691, 1736, 2500
BmrFI	CCNGG	5 cut(s)	1081, 1354, 1441, 2078, 2859
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	3 cut(s)	99, 487, 2702
BmuI	ACTGGG	1 cut(s)	166
BpiI	GAAGAC	3 cut(s)	1952, 2025, 2478
BpmI	CTGGAG	6 cut(s)	213, 522, 995, 1896, 2012, 2841
BpuEI	CTTGAG	3 cut(s)	98, 878, 1382
BpuMI	CCSGG	4 cut(s)	1081, 1354, 1441, 2078
Bsa29I	ATCGAT	1 cut(s)	2556
BsaHI	GRCGYC	1 cut(s)	408
BsaI	GGTCTC	1 cut(s)	1839
BsaJI	CCNNGG	2 cut(s)	2077, 2334
BsaWI	WCCGGW	6 cut(s)	239, 439, 730, 863, 1064, 1162
BsaXI	ACNNNNNCTCC	6 cut(s)	167, 197, 1421, 1451, 2827, 2857
Bse118I	RCCGGY	3 cut(s)	719, 762, 1064
Bse1I	ACTGG	6 cut(s)	12, 161, 505, 1879, 2029, 2609
Bse3DI	GCAATG	3 cut(s)	306, 1310, 2511
BseAI	TCCGGA	1 cut(s)	1162
BseBI	CCWGG	1 cut(s)	2859
BseCI	ATCGAT	1 cut(s)	2556
BseDI	CCNNGG	2 cut(s)	2077, 2334
BseGI	GGATG	5 cut(s)	681, 1379, 2392, 2551, 2684
BseMI	GCAATG	3 cut(s)	306, 1310, 2511
BseMII	CTCAG	4 cut(s)	654, 1011, 2693, 2740
BseNI	ACTGG	6 cut(s)	12, 161, 505, 1879, 2029, 2609
BseRI	GAGGAG	1 cut(s)	2835
BseXI	GCAGC	4 cut(s)	1830, 2106, 2178, 2317
BseYI	CCCAGC	2 cut(s)	1738, 2194
BsgI	GTGCAG	1 cut(s)	1837
BshFI	GGCC	9 cut(s)	132, 350, 432, 552, 704, 939, 1032, 1156, 2278
BshNI	GGYRCC	2 cut(s)	1325, 2498
BshTI	ACCGGT	1 cut(s)	1064
BshVI	ATCGAT	1 cut(s)	2556
BsiHKAI	GWGCWC	5 cut(s)	450, 894, 1499, 1843, 1882
BsiHKCI	CYCGRG	4 cut(s)	341, 1092, 1190, 1842
BslFI	GGGAC	6 cut(s)	173, 1240, 2095, 2175, 2274, 2380
BsmAI	GTCTC	2 cut(s)	887, 1839
BsmFI	GGGAC	6 cut(s)	173, 1240, 2095, 2175, 2274, 2380
BsmI	GAATGC	1 cut(s)	1771
BsnI	GGCC	9 cut(s)	132, 350, 432, 552, 704, 939, 1032, 1156, 2278
Bso31I	GGTCTC	1 cut(s)	1839
BsoBI	CYCGRG	4 cut(s)	341, 1092, 1190, 1842
Bsp1286I	GDGCHC	6 cut(s)	450, 570, 894, 1499, 1843, 1882
Bsp13I	TCCGGA	1 cut(s)	1162
Bsp1407I	TGTACA	2 cut(s)	1680, 2562
Bsp143I	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
BspACI	CCGC	3 cut(s)	486, 1802, 2188
BspANI	GGCC	9 cut(s)	132, 350, 432, 552, 704, 939, 1032, 1156, 2278
BspCNI	CTCAG	4 cut(s)	655, 1012, 2692, 2739
BspDI	ATCGAT	1 cut(s)	2556
BspEI	TCCGGA	1 cut(s)	1162
BspLI	GGNNCC	9 cut(s)	16, 133, 161, 162, 1327, 1393, 1691, 1736, 2500
BspMAI	CTGCAG	1 cut(s)	2124
BspPI	GGATC	5 cut(s)	849, 1713, 1729, 1742, 2136
BspQI	GCTCTTC	1 cut(s)	2803
BspT107I	GGYRCC	2 cut(s)	1325, 2498
BspTNI	GGTCTC	1 cut(s)	1839
BsrDI	GCAATG	3 cut(s)	306, 1310, 2511
BsrFI	RCCGGY	3 cut(s)	719, 762, 1064
BsrGI	TGTACA	2 cut(s)	1680, 2562
BsrI	ACTGG	6 cut(s)	12, 161, 505, 1879, 2029, 2609
BssAI	RCCGGY	3 cut(s)	719, 762, 1064
BssECI	CCNNGG	2 cut(s)	2077, 2334
BssMI	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
BssNI	GRCGYC	1 cut(s)	408
BssSI	CACGAG	2 cut(s)	216, 1836
BssT1I	CCWWGG	1 cut(s)	2334
Bst2BI	CACGAG	2 cut(s)	216, 1836
Bst2UI	CCWGG	1 cut(s)	2859
Bst4CI	ACNGT	7 cut(s)	105, 560, 785, 929, 1987, 2363, 2528
Bst6I	CTCTTC	3 cut(s)	172, 1934, 2803
BstACI	GRCGYC	1 cut(s)	408
BstAPI	GCANNNNNTGC	1 cut(s)	34
BstAUI	TGTACA	2 cut(s)	1680, 2562
BstC8I	GCNNGC	3 cut(s)	706, 2451, 2493
BstDEI	CTNAG	6 cut(s)	663, 1020, 1478, 2679, 2726, 2874
BstEII	GGTNACC	2 cut(s)	89, 1067
BstENI	CCTNNNNNAGG	1 cut(s)	2283
BstF5I	GGATG	5 cut(s)	681, 1379, 2392, 2551, 2684
BstKTI	GATC	8 cut(s)	403, 844, 964, 1269, 1461, 1708, 1737, 2131
BstMAI	GTCTC	2 cut(s)	887, 1839
BstMBI	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
BstMWI	GCNNNNNNNGC	5 cut(s)	34, 945, 1891, 2327, 2435
BstNI	CCWGG	1 cut(s)	2859
BstNSI	RCATGY	3 cut(s)	2206, 2245, 2485
BstPI	GGTNACC	2 cut(s)	89, 1067
BstSCI	CCNGG	5 cut(s)	1079, 1352, 1439, 2076, 2857
BstSFI	CTRYAG	2 cut(s)	773, 2120
BstV1I	GCAGC	4 cut(s)	1830, 2106, 2178, 2317
BstV2I	GAAGAC	3 cut(s)	1952, 2025, 2478
BstX2I	RGATCY	4 cut(s)	841, 1458, 1705, 1734
BstYI	RGATCY	4 cut(s)	841, 1458, 1705, 1734
Bsu15I	ATCGAT	1 cut(s)	2556
BsuRI	GGCC	9 cut(s)	132, 350, 432, 552, 704, 939, 1032, 1156, 2278
BsuTUI	ATCGAT	1 cut(s)	2556
BtgZI	GCGATG	1 cut(s)	2786
BtsCI	GGATG	5 cut(s)	681, 1379, 2392, 2551, 2684
BtsIMutI	CAGTG	5 cut(s)	101, 498, 1872, 2036, 2734
Cac8I	GCNNGC	3 cut(s)	706, 2451, 2493
Cfr10I	RCCGGY	3 cut(s)	719, 762, 1064
Cfr13I	GGNCC	9 cut(s)	131, 160, 431, 550, 938, 1154, 1658, 2276, 2468
ClaI	ATCGAT	1 cut(s)	2556
CseI	GACGC	3 cut(s)	107, 416, 2770
CspAI	ACCGGT	1 cut(s)	1064
CspCI	CAANNNNNGTGG	6 cut(s)	648, 683, 1492, 1527, 1650, 1685
DdeI	CTNAG	6 cut(s)	663, 1020, 1478, 2679, 2726, 2874
DpnI	GATC	8 cut(s)	402, 843, 963, 1268, 1460, 1707, 1736, 2130
DpnII	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
DraI	TTTAAA	2 cut(s)	366, 1407
DrdI	GACNNNNNNGTC	1 cut(s)	1853
DseDI	GACNNNNNNGTC	1 cut(s)	1853
EaeI	YGGCCR	3 cut(s)	348, 702, 1030
Eam1104I	CTCTTC	3 cut(s)	172, 1934, 2803
EarI	CTCTTC	3 cut(s)	172, 1934, 2803
EciI	GGCGGA	1 cut(s)	1817
Ecl136II	GAGCTC	4 cut(s)	448, 892, 1841, 1880
Eco130I	CCWWGG	1 cut(s)	2334
Eco24I	GRGCYC	5 cut(s)	450, 570, 894, 1843, 1882
Eco31I	GGTCTC	1 cut(s)	1839
Eco47I	GGWCC	3 cut(s)	160, 1658, 2468
Eco53kI	GAGCTC	4 cut(s)	448, 892, 1841, 1880
Eco57I	CTGAAG	1 cut(s)	1447
Eco88I	CYCGRG	4 cut(s)	341, 1092, 1190, 1842
Eco91I	GGTNACC	2 cut(s)	89, 1067
EcoICRI	GAGCTC	4 cut(s)	448, 892, 1841, 1880
EcoNI	CCTNNNNNAGG	1 cut(s)	2283
EcoO109I	RGGNCCY	1 cut(s)	160
EcoO65I	GGTNACC	2 cut(s)	89, 1067
EcoRI	GAATTC	2 cut(s)	654, 1673
EcoRII	CCWGG	1 cut(s)	2857
EcoT14I	CCWWGG	1 cut(s)	2334
EcoT22I	ATGCAT	1 cut(s)	2695
EcoT38I	GRGCYC	5 cut(s)	450, 570, 894, 1843, 1882
ErhI	CCWWGG	1 cut(s)	2334
FaqI	GGGAC	6 cut(s)	173, 1240, 2095, 2175, 2274, 2380
FauI	CCCGC	1 cut(s)	479
FauNDI	CATATG	1 cut(s)	2757
FbaI	TGATCA	1 cut(s)	1266
FblI	GTMKAC	2 cut(s)	2073, 2826
Fnu4HI	GCNGC	5 cut(s)	1819, 2120, 2189, 2192, 2331
FokI	GGATG	5 cut(s)	688, 1366, 2399, 2558, 2671
FriOI	GRGCYC	5 cut(s)	450, 570, 894, 1843, 1882
Fsp4HI	GCNGC	5 cut(s)	1819, 2120, 2189, 2192, 2331
FspBI	CTAG	8 cut(s)	149, 740, 1421, 1559, 2591, 2621, 2642, 2721
GluI	GCNGC	5 cut(s)	1819, 2120, 2189, 2192, 2331
GsaI	CCCAGC	2 cut(s)	1742, 2198
GsuI	CTGGAG	6 cut(s)	213, 522, 995, 1896, 2012, 2841
HaeIII	GGCC	9 cut(s)	132, 350, 432, 552, 704, 939, 1032, 1156, 2278
HgaI	GACGC	3 cut(s)	107, 416, 2770
Hin1I	GRCGYC	1 cut(s)	408
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HindIII	AAGCTT	2 cut(s)	332, 535
HphI	GGTGA	8 cut(s)	101, 295, 709, 835, 853, 1069, 1526, 2111
Hpy166II	GTNNAC	9 cut(s)	298, 1429, 1469, 1484, 2074, 2563, 2638, 2716, 2827
Hpy8I	GTNNAC	9 cut(s)	298, 1429, 1469, 1484, 2074, 2563, 2638, 2716, 2827
HpyAV	CCTTC	7 cut(s)	74, 196, 594, 666, 1035, 1772, 2859
HpyCH4III	ACNGT	7 cut(s)	105, 560, 785, 929, 1987, 2363, 2528
HpyCH4IV	ACGT	4 cut(s)	234, 624, 1553, 2022
HpyF10VI	GCNNNNNNNGC	5 cut(s)	34, 945, 1891, 2327, 2435
HpyF3I	CTNAG	6 cut(s)	663, 1020, 1478, 2679, 2726, 2874
HpySE526I	ACGT	4 cut(s)	234, 624, 1553, 2022
Hsp92I	GRCGYC	1 cut(s)	408
KflI	GGGWCCC	1 cut(s)	160
Kpn2I	TCCGGA	1 cut(s)	1162
KpnI	GGTACC	1 cut(s)	2502
Ksp22I	TGATCA	1 cut(s)	1266
Kzo9I	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
LguI	GCTCTTC	1 cut(s)	2803
LmnI	GCTCC	8 cut(s)	20, 42, 188, 713, 889, 1689, 1877, 2860
Lsp1109I	GCAGC	4 cut(s)	1830, 2106, 2178, 2317
LweI	GCATC	3 cut(s)	99, 487, 2702
MaeI	CTAG	8 cut(s)	149, 740, 1421, 1559, 2591, 2621, 2642, 2721
MaeII	ACGT	4 cut(s)	234, 624, 1553, 2022
MaeIII	GTNAC	8 cut(s)	89, 203, 629, 1060, 1067, 2507, 2593, 2644
MalI	GATC	8 cut(s)	402, 843, 963, 1268, 1460, 1707, 1736, 2130
MboI	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
MfeI	CAATTG	1 cut(s)	1472
MflI	RGATCY	4 cut(s)	841, 1458, 1705, 1734
MhlI	GDGCHC	6 cut(s)	450, 570, 894, 1499, 1843, 1882
MlsI	TGGCCA	2 cut(s)	350, 704
MluNI	TGGCCA	2 cut(s)	350, 704
MlyI	GAGTC	5 cut(s)	689, 1033, 1863, 2408, 2711
MmeI	TCCRAC	3 cut(s)	408, 735, 1846
Mox20I	TGGCCA	2 cut(s)	350, 704
Mph1103I	ATGCAT	1 cut(s)	2695
MroI	TCCGGA	1 cut(s)	1162
MroXI	GAANNNNTTC	3 cut(s)	494, 1433, 2022
MscI	TGGCCA	2 cut(s)	350, 704
MseI	TTAA	7 cut(s)	365, 852, 1118, 1248, 1406, 1632, 2151
MslI	CAYNNNNRTG	2 cut(s)	1682, 2385
Msp20I	TGGCCA	2 cut(s)	350, 704
MspA1I	CMGCKG	2 cut(s)	2119, 2144
MspR9I	CCNGG	5 cut(s)	1081, 1354, 1441, 2078, 2859
MunI	CAATTG	1 cut(s)	1472
Mva1269I	GAATGC	1 cut(s)	1771
MvaI	CCWGG	1 cut(s)	2859
MwoI	GCNNNNNNNGC	5 cut(s)	34, 945, 1891, 2327, 2435
NciI	CCSGG	4 cut(s)	1081, 1354, 1441, 2078
NdeI	CATATG	1 cut(s)	2757
NdeII	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
NlaIV	GGNNCC	9 cut(s)	16, 133, 161, 162, 1327, 1393, 1691, 1736, 2500
NmeAIII	GCCGAG	1 cut(s)	577
NmuCI	GTSAC	2 cut(s)	89, 203
NsiI	ATGCAT	1 cut(s)	2695
NspI	RCATGY	3 cut(s)	2206, 2245, 2485
PaeR7I	CTCGAG	3 cut(s)	341, 1092, 1842
PciI	ACATGT	1 cut(s)	2202
PciSI	GCTCTTC	1 cut(s)	2803
PctI	GAATGC	1 cut(s)	1771
PdmI	GAANNNNTTC	3 cut(s)	494, 1433, 2022
PflMI	CCANNNNNTGG	1 cut(s)	2096
PfoI	TCCNGGA	1 cut(s)	1352
PinAI	ACCGGT	1 cut(s)	1064
PkrI	GCNGC	5 cut(s)	1820, 2121, 2190, 2193, 2332
PleI	GAGTC	5 cut(s)	688, 1032, 1863, 2408, 2711
PpsI	GAGTC	5 cut(s)	688, 1032, 1863, 2408, 2711
PpuMI	RGGWCCY	1 cut(s)	160
PscI	ACATGT	1 cut(s)	2202
Psp124BI	GAGCTC	4 cut(s)	450, 894, 1843, 1882
Psp5II	RGGWCCY	1 cut(s)	160
Psp6I	CCWGG	1 cut(s)	2857
PspEI	GGTNACC	2 cut(s)	89, 1067
PspFI	CCCAGC	2 cut(s)	1738, 2194
PspGI	CCWGG	1 cut(s)	2857
PspN4I	GGNNCC	9 cut(s)	16, 133, 161, 162, 1327, 1393, 1691, 1736, 2500
PspPI	GGNCC	9 cut(s)	131, 160, 431, 550, 938, 1154, 1658, 2276, 2468
PspPPI	RGGWCCY	1 cut(s)	160
PsrI	GAACNNNNNNTAC	2 cut(s)	917, 949
PstI	CTGCAG	1 cut(s)	2124
PsuI	RGATCY	4 cut(s)	841, 1458, 1705, 1734
PvuII	CAGCTG	2 cut(s)	2119, 2144
RseI	CAYNNNNRTG	2 cut(s)	1682, 2385
SacI	GAGCTC	4 cut(s)	450, 894, 1843, 1882
SapI	GCTCTTC	1 cut(s)	2803
SaqAI	TTAA	7 cut(s)	365, 852, 1118, 1248, 1406, 1632, 2151
SatI	GCNGC	5 cut(s)	1819, 2120, 2189, 2192, 2331
Sau3AI	GATC	8 cut(s)	400, 841, 961, 1266, 1458, 1705, 1734, 2128
Sau96I	GGNCC	9 cut(s)	131, 160, 431, 550, 938, 1154, 1658, 2276, 2468
SchI	GAGTC	5 cut(s)	689, 1033, 1863, 2408, 2711
ScrFI	CCNGG	5 cut(s)	1081, 1354, 1441, 2078, 2859
SduI	GDGCHC	6 cut(s)	450, 570, 894, 1499, 1843, 1882
SfaNI	GCATC	3 cut(s)	99, 487, 2702
SfcI	CTRYAG	2 cut(s)	773, 2120
Sfr274I	CTCGAG	3 cut(s)	341, 1092, 1842
SinI	GGWCC	3 cut(s)	160, 1658, 2468
SlaI	CTCGAG	3 cut(s)	341, 1092, 1842
SmiMI	CAYNNNNRTG	2 cut(s)	1682, 2385
SmlI	CTYRAG	6 cut(s)	113, 341, 893, 1092, 1397, 1842
SmoI	CTYRAG	6 cut(s)	113, 341, 893, 1092, 1397, 1842
SpeI	ACTAGT	2 cut(s)	2590, 2620
SsiI	CCGC	3 cut(s)	486, 1802, 2188
SspI	AATATT	1 cut(s)	1507
SspMI	CTAG	8 cut(s)	149, 740, 1421, 1559, 2591, 2621, 2642, 2721
SstI	GAGCTC	4 cut(s)	450, 894, 1843, 1882
StyD4I	CCNGG	5 cut(s)	1079, 1352, 1439, 2076, 2857
StyI	CCWWGG	1 cut(s)	2334
TaaI	ACNGT	7 cut(s)	105, 560, 785, 929, 1987, 2363, 2528
TaiI	ACGT	4 cut(s)	237, 627, 1556, 2025
TatI	WGTACW	5 cut(s)	1680, 1996, 2238, 2562, 2701
TauI	GCSGC	1 cut(s)	2191
Tru1I	TTAA	7 cut(s)	365, 852, 1118, 1248, 1406, 1632, 2151
Tru9I	TTAA	7 cut(s)	365, 852, 1118, 1248, 1406, 1632, 2151
TscAI	CASTG	5 cut(s)	108, 505, 1879, 2036, 2734
TseFI	GTSAC	2 cut(s)	89, 203
TseI	GCWGC	4 cut(s)	1818, 2119, 2191, 2330
Tsp45I	GTSAC	2 cut(s)	89, 203
TspDTI	ATGAA	6 cut(s)	40, 189, 794, 943, 1666, 1714
TspGWI	ACGGA	1 cut(s)	588
TspRI	CASTG	5 cut(s)	108, 505, 1879, 2036, 2734
Van91I	CCANNNNNTGG	1 cut(s)	2096
VpaK11BI	GGWCC	3 cut(s)	160, 1658, 2468
XagI	CCTNNNNNAGG	1 cut(s)	2283
XapI	RAATTY	8 cut(s)	507, 654, 1338, 1402, 1518, 1673, 1699, 2221
XceI	RCATGY	3 cut(s)	2206, 2245, 2485
XhoI	CTCGAG	3 cut(s)	341, 1092, 1842
XmiI	GTMKAC	2 cut(s)	2073, 2826
XmnI	GAANNNNTTC	3 cut(s)	494, 1433, 2022
XspI	CTAG	8 cut(s)	149, 740, 1421, 1559, 2591, 2621, 2642, 2721
Zsp2I	ATGCAT	1 cut(s)	2695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.