Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000461

Physical Location & Seq

Genomic Coordinates Forward (+)

591703 .. 595205

Gene Structure 3503 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1010800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2922 bp

ATGGTAACCTCCAGTCCCCGCCGAATTCTCCCCCTTTGTTTCGTCGTTTTACTGCTTTGCTCCGCCCCCTGCGGCGGACACCGCGACCTCGATGCGCTGTTGAAGCTCAAGGCCGCAATGGTCGGCCCCAAAGGTTCTGGGCTCGAGGACTGGAACTCTTCGTCCTCGCACTGCTTCTTTTCTGGTGTTTTATGCGACCGCGACTCCAGAGTCGTTGCTCTCAATGTTTCGAATATTCCCCTGTTCGGCACGATTCCGGCGGCGATTGGGCTGCTGGACAAGCTCGTCAACCTCGAAATTACCGACGACAACTTGACCGGGAGGCTTCCGGCCGAGATGGCCAACCTCACCTCTCTCAAGCATCTGAACATCTCCAACAACGCGTTCAGCGGCAGCTTCCCCGGCGAAATCGTGCTCGGGATGACGGACCTTGAAGTCCTCGATGCCTACAACAACAACTTCAATGGGACACTTCCCATTCAGCTCGTCAGTCTCAAAAACATCAAGCACCTCCATCTTGGCGGGAACTACATCACCGGCGAAATTCCCGAGGATTACTCGGAGATACAGAGCTTGGAGTATTTGGGACTCAACGGTAATTTGCTCACCGGAAAGTTACCGGCGAGCTTAAGTAGGTTGAAGAATCTCAGGGAAATGTACGTAGGGTACTATAACAGTTACGACGGCGGAATTCCGCCGGAGTTGGGGTCGGTGAGCTCGCTGCAAGTCCTCGACATGAGCAGCTGCAACCTCGTGGGTCCCATCCCCACCACTCTCAGCCTTTTGAAACACTTGCACTCTCTTTTTTTGCAGGTGAACCGCCTCAGCGGCAGCATTCCGCCGCAGCTCTCGGCGTTGAACATGCTCATGTCTCTGGATCTCTCCATCAACGAGCTCACCGGAGAGATACCTGAGAGCTTCTCGGAGCTCAAGAACCTCACGCTGGTCAATCTGTACAAGAACAATCTCTACGGTCCGATTCCAAAGTTCGTAGGCGATTTTCCTCATCTCGAGGTGCTTCAAATTTGGGAGAACAACTTCACATTCGAGCTGCCGGAGAACCTCGGCCGGAACGGCAGGCTGAAGGACCTCGATGTCACCGGAAACCACCTCACCGGGCTGATTCCGCGGGATTTGTGCAAAGGAGGTAATTTGAAAACGGCAATTCTAATGGAAAATCACTTTTTCGGGCCGATTCCCGAGGAACTCGGGCTGTGCAATTCGCTGGTGAAAATCCGAATGATGAAGAACACACTCACCGGGACGATTCCAGCTGGGATATTCAGTTTGCCCAATTTGATCATGATCGAGCTAAACGACAATTTCTTGTCCGGCGAATTGCCACAGCAAATTTCTGGGGGCAATATTGGAATCCTCACACTTTCGGGAAATCACATTTCCGGGAAAATTCCGCCGGCGATTGGTAATCTCAAGAGTCTGCAAACTCTTTCCCTGGAGATGAACAGATTTTCCGGTGAAATTCCGACGGAAATTTTTTATTTAAAGTTGCTATCAAAGATCAATATCAGCGCCAACAACCTCAGCAGCGACATTTCAGAGTCCATTTCCCGCTGTTCCTCCCTGACGTCCGTTGATCTCAGTGGAAACAATTTGGTTGGCGAAATCCCGAGAGGAATTGCGAAGCTGAAAGTCCTGAGCATTCTCAATTTCTCCAGAAACCAACTCACCGGAGAAATTCCCGCCGAAATGAGATCCATGACGAGCCTCACGACTCTCGACCTCTCCAACAACAATTTCGTCGGCAGGCTCCCAACCGGCGGACAGTTTCTGGTCTTCAACGACACGTCGTTTGCGGGAAACCCCTATCTTTGCTCCCCGCGCCGCGTCCAGTGTCCGTCGTTCCATAGCCGCAAACCGTTCGCTACCTCCAAGATCGCTCTTATCGTCATCGGACTCTGTACGATTCTGTTATTCTTGTTCATAACGGCTTACCGGATGAGTAAGAGTGAAATCCAGAAATCTTTGGTGTGGCGTCTCACCGCCTTCCAGCGCCTCGATTTCGGAGCAGAGGACGTCCTCGAGTGCCTTAAAGAAGAGAACATCATAGGGAAAGGCGGCGCCGGGATCGTGTACCGCGGGTCGATGCCGGACGGCGTTGACGTGGCGATCAAACGGTTAGTCGGGCGAGGAACCGGGCGAAACGACCACGGTTTCTCGGCCGAGATAAAGACTCTGGGAAGAATCCGGCACCGGAATATCGTGAGGCTGTTGGGGTACGTGTCGAACAAGGACACGAATCTGCTGCTGTACGAGTACATGCCAAATGGGAGCTTGGGGGAGCTGCTGCATGGGCCGAAAGGGGGGCATTTGCAGTGGGAGAGGAGGTACAGGATCGCCGTCGAGGCTGCCAAGGGGCTGTGTTATCTCCACCACGACTGTTCGCCGCTGATTATTCACAGGGACGTTAAGTCCAATAATATTTTGCTGGACTCGGATTTGGAAGCCCACGTTGCGGATTTTGGGCTCGCTAAGTTTTTGCAGGATGCTGGGGCGTCCGAGTGCATGTCTTCCGTTGCTGGGTCCTATGGTTACATTGCCCCAGAGTACGCCTACACATTGAAAGTGGACGAAAAAAGCGACGTGTACAGCTTCGGCGTGGTGCTGCTGGAGTTAATAGCAGGGAGGAAGCCGGTGGGAGAATTCGGAGACGGGGTGGACATAGTAAGATGGGTGAGGAAGACAACATCGGAGCTCTCTCAGCCATCTGATGCAGCGTCGGTACTGGCGGTGGTCGACCACAGGCTCTCCGGGTACCCACTGGCAGGCGTGGTACACCTGTTCAAGATTGCCATGATGAGCGTAGAGGATGAGAGCTCCGCCAGGCCAACGATGAGGGAAGTGGTGCACATGCTCACCAATCCTCCGCCCGCGGCTCCAACTCCGAGCCTGCTTAACCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000325 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009506 GO:0009791 GO:0009888 GO:0009908 GO:0009909 GO:0009934 GO:0009987 GO:0010073 GO:0010075 GO:0010080 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022414 GO:0022603 GO:0030054 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0036289 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048831 GO:0048832 GO:0048833 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0140096 GO:1901564 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

974

Amino Acids

106.17

Weight (kDa)

6.31

Isoelectric Point (pI)

34.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	28 - 66	8.2e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	146 - 269	2.1e-06	Leucine-rich repeat region
LRR_14	PF23598	233 - 371	6.1e-09	Leucine-rich repeat region
LRR_8	PF13855	479 - 538	5.3e-06	Leucine rich repeat
LRR_8	PF13855	527 - 586	1.1e-07	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	685 - 913	2e-41	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	685 - 886	4.9e-41	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000461)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75820
fragaria_vesca	FvH4_2g38230 FvH4_2g38230
malus_domestica	MD05G1138900.v1.1 MD05G1139100.v1.1 MD05G1139200.v1.1 MD08G1010700.v1.1 MD08G1241800.v1.1 MD15G1010800.v1.1
prunus_persica	Prupe.1G363300_v2.0.a1 Prupe.6G163000_v2.0.a1
pyrus_communis	pycom05g13420 pycom05g13430 pycom08g00930 pycom15g00920
rosa_chinensis	RchiOBHm_Chr2g0118991 RchiOBHm_Chr2g0119001 RchiOBHm_Chr2g0119031 RchiOBHm_Chr2g0119041 RchiOBHm_Chr2g0119051 RchiOBHm_Chr2g0119091 RchiOBHm_Chr2g0119101 RchiOBHm_Chr2g0119111 RchiOBHm_Chr2g0120661 RchiOBHm_Chr2g0173321 RchiOBHm_Chr3g0478281 RchiOBHm_Chr4g0421131 RchiOBHm_Chr6g0303151
rosa_laevigata	RLG00000011084 RLG00000011103 RLG00000018441 RLG00000018445 RLG00000018565 RLG00000018566 RLG00000018567 RLG00000018568 RLG00000018571
rosa_multiflora	Rmu_co8157004.1_g000001 Rmu_co8277963.1_g000001 Rmu_co8302039.1_g000001 Rmu_co8360703.1_g000001 Rmu_co8384839.1_g000001 Rmu_sc0000319.1_g000027 Rmu_sc0001653.1_g000012 Rmu_sc0001653.1_g000013 Rmu_sc0001653.1_g000021 Rmu_sc0001822.1_g000002 Rmu_sc0001822.1_g000008 Rmu_sc0001822.1_g000030 Rmu_sc0001844.1_g000003 Rmu_sc0008911.1_g000007 Rmu_sc0019499.1_g000001 Rmu_sc0025689.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123240 Rroxscaffold_2G00123260 Rroxscaffold_2G00124530 Rroxscaffold_7G00164970 Rroxscaffold_7G00192210
rosa_rugosa	Rorug02G0220100 Rorug02G0220200 Rorug02G0230200 Rorug02G0230300 Rorug06G0319400
rosa_samantha	Rh2AG277100 Rh2AG277200 Rh2AG277700 Rh2AG277800 Rh2AG278100 Rh2AG278300 Rh2AG286600 Rh2BG289000 Rh2BG289200 Rh2BG298100 Rh2BG298200 Rh2CG275700 Rh2CG275900 Rh2CG623600 Rh2DG284200 Rh2DG303300 Rh2DG303400 Rh2DG303500 Rh2DG312000 Rh2DG312100 Rh6AG433400 Rh6BG469300 Rh6CG445900 Rh6DG432500
rosa_wichuraiana	Rw2G022080 Rw2G022990 Rw2G023000 Rw6G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	802
AasI	GACNNNNNNGTC	4 cut(s)	209, 284, 434, 1094
AatII	GACGTC	2 cut(s)	1589, 2037
Acc36I	ACCTGC	1 cut(s)	802
Acc65I	GGTACC	1 cut(s)	2772
AccB1I	GGYRCC	3 cut(s)	2078, 2208, 2772
AccI	GTMKAC	1 cut(s)	2754
AccII	CGCG	8 cut(s)	84, 201, 383, 1129, 1840, 1845, 2097, 2891
AclWI	GGATC	4 cut(s)	885, 1707, 2093, 2360
AcoI	YGGCCR	4 cut(s)	330, 339, 1066, 2178
AcuI	CTGAAG	1 cut(s)	1103
AcyI	GRCGYC	5 cut(s)	1586, 1993, 2034, 2079, 2513
AflII	CTTAAG	1 cut(s)	628
AflIII	ACRYGT	4 cut(s)	381, 1803, 2238, 2601
AhdI	GACNNNNNGTC	1 cut(s)	2428
AjiI	CACGTC	3 cut(s)	1806, 2122, 2602
AjnI	CCWGG	2 cut(s)	1452, 2840
AjuI	GAANNNNNNNTTGG	4 cut(s)	368, 400, 1739, 1771
AloI	GAACNNNNNNTCC	4 cut(s)	146, 178, 1454, 1486
Alw21I	GWGCWC	7 cut(s)	417, 719, 897, 930, 2715, 2837, 2868
Alw26I	GTCTC	4 cut(s)	497, 876, 2000, 2661
Alw44I	GTGCAC	1 cut(s)	2864
AlwI	GGATC	4 cut(s)	885, 1707, 2093, 2360
AlwNI	CAGNNNCTG	1 cut(s)	1789
Ama87I	CYCGRG	8 cut(s)	143, 416, 548, 1010, 1199, 1208, 1627, 2039
AoxI	GGCC	9 cut(s)	111, 124, 330, 339, 1066, 1190, 2178, 2312, 2843
ApaLI	GTGCAC	1 cut(s)	2864
ArsI	GACNNNNNNTTYG	4 cut(s)	1793, 1825, 2124, 2156
Asp700I	GAANNNNTTC	1 cut(s)	1037
Asp718I	GGTACC	1 cut(s)	2772
AspLEI	GCGC	5 cut(s)	97, 1532, 1842, 2013, 2081
AspS9I	GGNCC	8 cut(s)	125, 427, 758, 974, 1087, 1190, 2312, 2541
AsuC2I	CCSGG	8 cut(s)	319, 402, 1117, 1261, 1402, 2083, 2155, 2770
AsuII	TTCGAA	1 cut(s)	230
AvaI	CYCGRG	8 cut(s)	143, 416, 548, 1010, 1199, 1208, 1627, 2039
AvaII	GGWCC	5 cut(s)	427, 758, 974, 1087, 2541
BaeGI	GKGCMC	1 cut(s)	2868
BaeI	ACNNNNGTAYC	2 cut(s)	2783, 2816
BalI	TGGCCA	1 cut(s)	341
BanI	GGYRCC	3 cut(s)	2078, 2208, 2772
BanII	GRGCYC	7 cut(s)	144, 719, 897, 930, 2487, 2715, 2837
BauI	CACGAG	1 cut(s)	752
BbsI	GAAGAC	3 cut(s)	1786, 2520, 2705
Bbv12I	GWGCWC	7 cut(s)	417, 719, 897, 930, 2715, 2837, 2868
BbvCI	CCTCAGC	2 cut(s)	824, 1541
BccI	CCATC	6 cut(s)	331, 522, 770, 893, 2682, 2731
BceAI	ACGGC	6 cut(s)	700, 1090, 1176, 1962, 2128, 2342
BcgI	CGANNNNNNTGC	8 cut(s)	74, 108, 253, 287, 1861, 1895, 2245, 2279
BciT130I	CCWGG	2 cut(s)	1454, 2842
BclI	TGATCA	1 cut(s)	1299
BcnI	CCSGG	8 cut(s)	319, 402, 1117, 1261, 1402, 2083, 2155, 2770
BcoDI	GTCTC	4 cut(s)	497, 876, 2000, 2661
BfoI	RGCGCY	3 cut(s)	1533, 2014, 2082
BfrI	CTTAAG	1 cut(s)	628
BfuAI	ACCTGC	1 cut(s)	802
BglI	GCCNNNNNGGC	4 cut(s)	338, 828, 1074, 2363
Bme18I	GGWCC	5 cut(s)	427, 758, 974, 1087, 2541
BmeRI	GACNNNNNGTC	1 cut(s)	2428
BmeT110I	CYCGRG	8 cut(s)	143, 416, 548, 1010, 1199, 1208, 1627, 2039
BmgBI	CACGTC	3 cut(s)	1806, 2122, 2602
BmgT120I	GGNCC	8 cut(s)	125, 427, 758, 974, 1087, 1190, 2312, 2541
BmsI	GCATC	6 cut(s)	82, 370, 433, 2094, 2494, 2719
BpiI	GAAGAC	3 cut(s)	1786, 2520, 2705
BplI	GAGNNNNNCTC	4 cut(s)	542, 574, 905, 937
BpmI	CTGGAG	4 cut(s)	190, 1475, 1657, 2648
Bpu10I	CCTNAGC	3 cut(s)	824, 1541, 1655
Bpu14I	TTCGAA	1 cut(s)	230
BpuEI	CTTGAG	4 cut(s)	92, 341, 914, 1415
BpuMI	CCSGG	8 cut(s)	319, 402, 1117, 1261, 1402, 2083, 2155, 2770
BsaAI	YACGTR	2 cut(s)	661, 2239
BsaBI	GATNNNNATC	2 cut(s)	1304, 1523
BsaHI	GRCGYC	5 cut(s)	1586, 1993, 2034, 2079, 2513
BsaWI	WCCGGW	7 cut(s)	608, 899, 1100, 1472, 1688, 1953, 2211
Bse118I	RCCGGY	5 cut(s)	536, 619, 1414, 1775, 2650
Bse1I	ACTGG	5 cut(s)	12, 155, 1849, 2748, 2784
Bse3DI	GCAATG	2 cut(s)	123, 2553
Bse8I	GATNNNNATC	2 cut(s)	1304, 1523
BseBI	CCWGG	2 cut(s)	1454, 2842
BseGI	GGATG	5 cut(s)	426, 762, 1962, 2509, 2833
BseJI	GATNNNNATC	2 cut(s)	1304, 1523
BseMI	GCAATG	2 cut(s)	123, 2553
BseMII	CTCAG	8 cut(s)	661, 790, 838, 903, 1555, 1612, 1646, 2732
BseNI	ACTGG	5 cut(s)	12, 155, 1849, 2748, 2784
BseRI	GAGGAG	1 cut(s)	2356
BseSI	GKGCMC	1 cut(s)	2868
BseX3I	CGGCCG	3 cut(s)	330, 1066, 2178
BseYI	CCCAGC	3 cut(s)	1274, 2507, 2537
Bsh1236I	CGCG	8 cut(s)	84, 201, 383, 1129, 1840, 1845, 2097, 2891
Bsh1285I	CGRYCG	4 cut(s)	199, 333, 1069, 2181
BshFI	GGCC	9 cut(s)	113, 126, 332, 341, 1068, 1192, 2180, 2314, 2845
BshNI	GGYRCC	3 cut(s)	2078, 2208, 2772
BsiEI	CGRYCG	4 cut(s)	199, 333, 1069, 2181
BsiHKAI	GWGCWC	7 cut(s)	417, 719, 897, 930, 2715, 2837, 2868
BsiHKCI	CYCGRG	8 cut(s)	143, 416, 548, 1010, 1199, 1208, 1627, 2039
BslFI	GGGAC	5 cut(s)	481, 600, 744, 1276, 2435
BsmAI	GTCTC	4 cut(s)	497, 876, 2000, 2661
BsmBI	CGTCTC	2 cut(s)	2000, 2661
BsmFI	GGGAC	5 cut(s)	481, 600, 744, 1276, 2435
BsmI	GAATGC	2 cut(s)	834, 1659
BsnI	GGCC	9 cut(s)	113, 126, 332, 341, 1068, 1192, 2180, 2314, 2845
BsoBI	CYCGRG	8 cut(s)	143, 416, 548, 1010, 1199, 1208, 1627, 2039
Bsp119I	TTCGAA	1 cut(s)	230
Bsp1286I	GDGCHC	9 cut(s)	144, 417, 719, 897, 930, 2487, 2715, 2837, 2868
Bsp1407I	TGTACA	2 cut(s)	954, 2604
BspANI	GGCC	9 cut(s)	113, 126, 332, 341, 1068, 1192, 2180, 2314, 2845
BspCNI	CTCAG	8 cut(s)	660, 789, 837, 904, 1554, 1611, 1647, 2731
BspFNI	CGCG	8 cut(s)	84, 201, 383, 1129, 1840, 1845, 2097, 2891
BspHI	TCATGA	1 cut(s)	1302
BspMI	ACCTGC	1 cut(s)	802
BspPI	GGATC	4 cut(s)	885, 1707, 2093, 2360
BspT104I	TTCGAA	1 cut(s)	230
BspT107I	GGYRCC	3 cut(s)	2078, 2208, 2772
BspTI	CTTAAG	1 cut(s)	628
BsrDI	GCAATG	2 cut(s)	123, 2553
BsrFI	RCCGGY	5 cut(s)	536, 619, 1414, 1775, 2650
BsrGI	TGTACA	2 cut(s)	954, 2604
BsrI	ACTGG	5 cut(s)	12, 155, 1849, 2748, 2784
BssAI	RCCGGY	5 cut(s)	536, 619, 1414, 1775, 2650
BssNI	GRCGYC	5 cut(s)	1586, 1993, 2034, 2079, 2513
BssSI	CACGAG	1 cut(s)	752
BssT1I	CCWWGG	1 cut(s)	2370
Bst2BI	CACGAG	1 cut(s)	752
Bst2UI	CCWGG	2 cut(s)	1454, 2842
Bst4CI	ACNGT	8 cut(s)	596, 677, 974, 1785, 1878, 2136, 2171, 2399
Bst6I	CTCTTC	2 cut(s)	163, 2049
BstACI	GRCGYC	5 cut(s)	1586, 1993, 2034, 2079, 2513
BstAFI	CTTAAG	1 cut(s)	628
BstAUI	TGTACA	2 cut(s)	954, 2604
BstBAI	YACGTR	2 cut(s)	661, 2239
BstBI	TTCGAA	1 cut(s)	230
BstC8I	GCNNGC	9 cut(s)	625, 719, 1079, 1416, 1766, 2487, 2785, 2889, 2909
BstDEI	CTNAG	9 cut(s)	647, 776, 824, 912, 1541, 1598, 1655, 2490, 2718
BstDSI	CCRYGG	4 cut(s)	1127, 2095, 2167, 2889
BstEII	GGTNACC	1 cut(s)	4
BstF5I	GGATG	5 cut(s)	426, 762, 1962, 2509, 2833
BstFNI	CGCG	8 cut(s)	84, 201, 383, 1129, 1840, 1845, 2097, 2891
BstH2I	RGCGCY	3 cut(s)	1533, 2014, 2082
BstHHI	GCGC	5 cut(s)	97, 1532, 1842, 2013, 2081
BstMAI	GTCTC	4 cut(s)	497, 876, 2000, 2661
BstMCI	CGRYCG	4 cut(s)	199, 333, 1069, 2181
BstNI	CCWGG	2 cut(s)	1454, 2842
BstNSI	RCATGY	4 cut(s)	865, 2281, 2527, 2872
BstPI	GGTNACC	1 cut(s)	4
BstSLI	GKGCMC	1 cut(s)	2868
BstSNI	TACGTA	1 cut(s)	661
BstUI	CGCG	8 cut(s)	84, 201, 383, 1129, 1840, 1845, 2097, 2891
BstV2I	GAAGAC	3 cut(s)	1786, 2520, 2705
BstX2I	RGATCY	2 cut(s)	877, 1712
BstYI	RGATCY	2 cut(s)	877, 1712
BstZI	CGGCCG	3 cut(s)	330, 1066, 2178
BsuRI	GGCC	9 cut(s)	113, 126, 332, 341, 1068, 1192, 2180, 2314, 2845
BtgI	CCRYGG	4 cut(s)	1127, 2095, 2167, 2889
BtrI	CACGTC	3 cut(s)	1806, 2122, 2602
BtsCI	GGATG	5 cut(s)	426, 762, 1962, 2509, 2833
BtsI	GCAGTG	2 cut(s)	169, 2339
BtsIMutI	CAGTG	5 cut(s)	169, 1606, 1856, 2339, 2777
BveI	ACCTGC	1 cut(s)	802
Cac8I	GCNNGC	9 cut(s)	625, 719, 1079, 1416, 1766, 2487, 2785, 2889, 2909
CaiI	CAGNNNCTG	1 cut(s)	1789
CciI	TCATGA	1 cut(s)	1302
CfoI	GCGC	5 cut(s)	97, 1532, 1842, 2013, 2081
Cfr10I	RCCGGY	5 cut(s)	536, 619, 1414, 1775, 2650
Cfr13I	GGNCC	8 cut(s)	125, 427, 758, 974, 1087, 1190, 2312, 2541
Cfr42I	CCGCGG	3 cut(s)	1130, 2098, 2892
CpoI	CGGWCCG	1 cut(s)	974
CseI	GACGC	4 cut(s)	1834, 1982, 2502, 2724
CspI	CGGWCCG	1 cut(s)	974
DdeI	CTNAG	9 cut(s)	647, 776, 824, 912, 1541, 1598, 1655, 2490, 2718
DinI	GGCGCC	1 cut(s)	2080
DraI	TTTAAA	1 cut(s)	1503
DrdI	GACNNNNNNGTC	4 cut(s)	209, 284, 434, 1094
DriI	GACNNNNNGTC	1 cut(s)	2428
DseDI	GACNNNNNNGTC	4 cut(s)	209, 284, 434, 1094
EaeI	YGGCCR	4 cut(s)	330, 339, 1066, 2178
EagI	CGGCCG	3 cut(s)	330, 1066, 2178
Eam1104I	CTCTTC	2 cut(s)	163, 2049
Eam1105I	GACNNNNNGTC	1 cut(s)	2428
EarI	CTCTTC	2 cut(s)	163, 2049
EciI	GGCGGA	9 cut(s)	52, 90, 684, 702, 828, 1401, 1794, 2827, 2874
Ecl136II	GAGCTC	5 cut(s)	717, 895, 928, 2713, 2835
EclXI	CGGCCG	3 cut(s)	330, 1066, 2178
Eco105I	TACGTA	1 cut(s)	661
Eco130I	CCWWGG	1 cut(s)	2370
Eco24I	GRGCYC	7 cut(s)	144, 719, 897, 930, 2487, 2715, 2837
Eco47I	GGWCC	5 cut(s)	427, 758, 974, 1087, 2541
Eco52I	CGGCCG	3 cut(s)	330, 1066, 2178
Eco53kI	GAGCTC	5 cut(s)	717, 895, 928, 2713, 2835
Eco57I	CTGAAG	1 cut(s)	1103
Eco88I	CYCGRG	8 cut(s)	143, 416, 548, 1010, 1199, 1208, 1627, 2039
Eco91I	GGTNACC	1 cut(s)	4
EcoICRI	GAGCTC	5 cut(s)	717, 895, 928, 2713, 2835
EcoO109I	RGGNCCY	3 cut(s)	758, 1087, 2541
EcoO65I	GGTNACC	1 cut(s)	4
EcoRI	GAATTC	3 cut(s)	24, 690, 2660
EcoRII	CCWGG	2 cut(s)	1452, 2840
EcoT14I	CCWWGG	1 cut(s)	2370
EcoT38I	GRGCYC	7 cut(s)	144, 719, 897, 930, 2487, 2715, 2837
EgeI	GGCGCC	1 cut(s)	2080
EheI	GGCGCC	1 cut(s)	2080
ErhI	CCWWGG	1 cut(s)	2370
Esp3I	CGTCTC	2 cut(s)	2000, 2661
FaqI	GGGAC	5 cut(s)	481, 600, 744, 1276, 2435
FauI	CCCGC	9 cut(s)	26, 515, 1122, 1577, 1708, 1807, 1845, 2090, 2896
FbaI	TGATCA	1 cut(s)	1299
FblI	GTMKAC	1 cut(s)	2754
FokI	GGATG	5 cut(s)	433, 749, 1969, 2516, 2840
FriOI	GRGCYC	7 cut(s)	144, 719, 897, 930, 2487, 2715, 2837
GlaI	GCGC	5 cut(s)	96, 1531, 1841, 2012, 2080
GsaI	CCCAGC	3 cut(s)	1278, 2511, 2541
GsuI	CTGGAG	4 cut(s)	190, 1475, 1657, 2648
HaeII	RGCGCY	3 cut(s)	1533, 2014, 2082
HaeIII	GGCC	9 cut(s)	113, 126, 332, 341, 1068, 1192, 2180, 2314, 2845
HgaI	GACGC	4 cut(s)	1834, 1982, 2502, 2724
HhaI	GCGC	5 cut(s)	97, 1532, 1842, 2013, 2081
Hin1I	GRCGYC	5 cut(s)	1586, 1993, 2034, 2079, 2513
Hin6I	GCGC	5 cut(s)	95, 1530, 1840, 2011, 2079
HinP1I	GCGC	5 cut(s)	95, 1530, 1840, 2011, 2079
HincII	GTYRAC	3 cut(s)	289, 2119, 2755
HindII	GTYRAC	3 cut(s)	289, 2119, 2755
HpyAV	CCTTC	2 cut(s)	1078, 2014
HpyCH4III	ACNGT	8 cut(s)	596, 677, 974, 1785, 1878, 2136, 2171, 2399
HpyCH4IV	ACGT	9 cut(s)	660, 1586, 1805, 2034, 2121, 2238, 2424, 2469, 2601
HpyF3I	CTNAG	9 cut(s)	647, 776, 824, 912, 1541, 1598, 1655, 2490, 2718
HpySE526I	ACGT	9 cut(s)	660, 1586, 1805, 2034, 2121, 2238, 2424, 2469, 2601
Hsp92I	GRCGYC	5 cut(s)	1586, 1993, 2034, 2079, 2513
HspAI	GCGC	5 cut(s)	95, 1530, 1840, 2011, 2079
KasI	GGCGCC	1 cut(s)	2078
KflI	GGGWCCC	1 cut(s)	758
KpnI	GGTACC	1 cut(s)	2776
KroI	GCCGGC	1 cut(s)	1414
KroNI	GCCGGC	1 cut(s)	1416
Ksp22I	TGATCA	1 cut(s)	1299
KspI	CCGCGG	3 cut(s)	1130, 2098, 2892
LweI	GCATC	6 cut(s)	82, 370, 433, 2094, 2494, 2719
MaeII	ACGT	9 cut(s)	660, 1586, 1805, 2034, 2121, 2238, 2424, 2469, 2601
MaeIII	GTNAC	5 cut(s)	4, 615, 677, 1096, 2549
MboII	GAAGA	8 cut(s)	150, 652, 1258, 1786, 2066, 2211, 2520, 2710
MflI	RGATCY	2 cut(s)	877, 1712
MhlI	GDGCHC	9 cut(s)	144, 417, 719, 897, 930, 2487, 2715, 2837, 2868
MlsI	TGGCCA	1 cut(s)	341
MluI	ACGCGT	1 cut(s)	381
MluNI	TGGCCA	1 cut(s)	341
Mly113I	GGCGCC	1 cut(s)	2079
MlyI	GAGTC	9 cut(s)	197, 219, 582, 1444, 1568, 1726, 1908, 2185, 2444
MmeI	TCCRAC	4 cut(s)	399, 1508, 1770, 2921
Mox20I	TGGCCA	1 cut(s)	341
MreI	CGCCGGCG	1 cut(s)	1414
MroNI	GCCGGC	1 cut(s)	1414
MroXI	GAANNNNTTC	1 cut(s)	1037
MscI	TGGCCA	1 cut(s)	341
MseI	TTAA	6 cut(s)	629, 1502, 2049, 2427, 2633, 2913
MslI	CAYNNNNRTG	1 cut(s)	866
Msp20I	TGGCCA	1 cut(s)	341
MspA1I	CMGCKG	9 cut(s)	390, 744, 828, 1129, 1274, 1572, 2097, 2407, 2891
MspCI	CTTAAG	1 cut(s)	628
Mva1269I	GAATGC	2 cut(s)	834, 1659
MvaI	CCWGG	2 cut(s)	1454, 2842
MvnI	CGCG	8 cut(s)	84, 201, 383, 1129, 1840, 1845, 2097, 2891
NaeI	GCCGGC	1 cut(s)	1416
NarI	GGCGCC	1 cut(s)	2079
NciI	CCSGG	8 cut(s)	319, 402, 1117, 1261, 1402, 2083, 2155, 2770
NgoMIV	GCCGGC	1 cut(s)	1414
NmeAIII	GCCGAG	5 cut(s)	358, 830, 1044, 2156, 2206
NmuCI	GTSAC	1 cut(s)	1096
NspI	RCATGY	4 cut(s)	865, 2281, 2527, 2872
NspV	TTCGAA	1 cut(s)	230
PaeR7I	CTCGAG	3 cut(s)	143, 1010, 2039
PagI	TCATGA	1 cut(s)	1302
PaqCI	CACCTGC	1 cut(s)	802
PcsI	WCGNNNNNNNCGW	7 cut(s)	291, 300, 1314, 1727, 1902, 2118, 2595
PctI	GAATGC	2 cut(s)	834, 1659
PdiI	GCCGGC	1 cut(s)	1416
PdmI	GAANNNNTTC	1 cut(s)	1037
PfoI	TCCNGGA	1 cut(s)	1400
PleI	GAGTC	9 cut(s)	197, 218, 582, 1443, 1567, 1726, 1908, 2185, 2444
PluTI	GGCGCC	1 cut(s)	2082
PpsI	GAGTC	9 cut(s)	197, 218, 582, 1443, 1567, 1726, 1908, 2185, 2444
Ppu21I	YACGTR	2 cut(s)	661, 2239
PpuMI	RGGWCCY	3 cut(s)	758, 1087, 2541
Psp124BI	GAGCTC	5 cut(s)	719, 897, 930, 2715, 2837
Psp5II	RGGWCCY	3 cut(s)	758, 1087, 2541
Psp6I	CCWGG	2 cut(s)	1452, 2840
PspEI	GGTNACC	1 cut(s)	4
PspFI	CCCAGC	3 cut(s)	1274, 2507, 2537
PspGI	CCWGG	2 cut(s)	1452, 2840
PspPI	GGNCC	8 cut(s)	125, 427, 758, 974, 1087, 1190, 2312, 2541
PspPPI	RGGWCCY	3 cut(s)	758, 1087, 2541
PspXI	VCTCGAGB	2 cut(s)	143, 2039
PsrI	GAACNNNNNNTAC	2 cut(s)	953, 985
PstNI	CAGNNNCTG	1 cut(s)	1789
PsuI	RGATCY	2 cut(s)	877, 1712
PvuII	CAGCTG	2 cut(s)	744, 1274
RseI	CAYNNNNRTG	1 cut(s)	866
Rsr2I	CGGWCCG	1 cut(s)	974
RsrII	CGGWCCG	1 cut(s)	974
SacI	GAGCTC	5 cut(s)	719, 897, 930, 2715, 2837
SacII	CCGCGG	3 cut(s)	1130, 2098, 2892
SalI	GTCGAC	1 cut(s)	2753
SaqAI	TTAA	6 cut(s)	629, 1502, 2049, 2427, 2633, 2913
Sau96I	GGNCC	8 cut(s)	125, 427, 758, 974, 1087, 1190, 2312, 2541
SchI	GAGTC	9 cut(s)	197, 219, 582, 1444, 1568, 1726, 1908, 2185, 2444
SduI	GDGCHC	9 cut(s)	144, 417, 719, 897, 930, 2487, 2715, 2837, 2868
SfaNI	GCATC	6 cut(s)	82, 370, 433, 2094, 2494, 2719
SfiI	GGCCNNNNNGGCC	1 cut(s)	338
SfoI	GGCGCC	1 cut(s)	2080
Sfr274I	CTCGAG	3 cut(s)	143, 1010, 2039
Sfr303I	CCGCGG	3 cut(s)	1130, 2098, 2892
SfuI	TTCGAA	1 cut(s)	230
SgrAI	CRCCGGYG	2 cut(s)	536, 1414
SgrBI	CCGCGG	3 cut(s)	1130, 2098, 2892
SinI	GGWCC	5 cut(s)	427, 758, 974, 1087, 2541
SlaI	CTCGAG	3 cut(s)	143, 1010, 2039
SmiMI	CAYNNNNRTG	1 cut(s)	866
SmlI	CTYRAG	8 cut(s)	107, 143, 356, 628, 929, 1010, 1430, 2039
SmoI	CTYRAG	8 cut(s)	107, 143, 356, 628, 929, 1010, 1430, 2039
SnaBI	TACGTA	1 cut(s)	661
SspDI	GGCGCC	1 cut(s)	2078
SspI	AATATT	3 cut(s)	235, 1366, 2440
SstI	GAGCTC	5 cut(s)	719, 897, 930, 2715, 2837
StyI	CCWWGG	1 cut(s)	2370
TaaI	ACNGT	8 cut(s)	596, 677, 974, 1785, 1878, 2136, 2171, 2399
TaiI	ACGT	9 cut(s)	663, 1589, 1808, 2037, 2124, 2241, 2427, 2472, 2604
TatI	WGTACW	3 cut(s)	954, 2274, 2604
Tru1I	TTAA	6 cut(s)	629, 1502, 2049, 2427, 2633, 2913
Tru9I	TTAA	6 cut(s)	629, 1502, 2049, 2427, 2633, 2913
TscAI	CASTG	5 cut(s)	176, 1606, 1856, 2339, 2784
TseFI	GTSAC	1 cut(s)	1096
Tsp45I	GTSAC	1 cut(s)	1096
TspDTI	ATGAA	3 cut(s)	1259, 1475, 1930
TspGWI	ACGGA	5 cut(s)	440, 1502, 1579, 1845, 2521
TspRI	CASTG	5 cut(s)	176, 1606, 1856, 2339, 2784
Vha464I	CTTAAG	1 cut(s)	628
VneI	GTGCAC	1 cut(s)	2864
VpaK11BI	GGWCC	5 cut(s)	427, 758, 974, 1087, 2541
XceI	RCATGY	4 cut(s)	865, 2281, 2527, 2872
XhoI	CTCGAG	3 cut(s)	143, 1010, 2039
XmiI	GTMKAC	1 cut(s)	2754
XmnI	GAANNNNTTC	1 cut(s)	1037
ZraI	GACGTC	2 cut(s)	1587, 2035

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.