RchiOBHm_Chr2g0150531

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000689
Physical Location & Seq
Forward (+)
68218939 .. 68219540
602 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51982

Sequence Viewer

Length: 390 bp
ATGCGCCATGGGGTCAAATTTCTACTTACCATGCAAAGAGAGAACGGTGGTTGGGGAGAGAGTTATCTTTCATGTCCACAAAAGACATACATTCCTCTTGAAGGAAACCGATCTAATCTAGTACAAACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAGGCAGAAAGAGACCCAACACCTCTTCATCATGCAGCAAAGTTTATAATCAATTCTCAACTGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCAAGAAGAACTGCATGCAACATTATGCAGCATATAGAAATATATACCCACTGTGGGCTCTGGCAGAATATTGTAATCGGGTTCCGTTGCCTTCCAAGGGCTGCATTGCTAACCGTAATATGTAG

Protein Analysis

129

Amino Acids

14.7

Weight (kDa)

8.98

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 1 - 113 7.2e-19 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000689)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08040
malus_domestica MD09G1245800.v1.1 MD11G1098500.v1.1 MD16G1237300.v1.1
pyrus_communis pycom03g07090 pycom03g07100 pycom09g08560 pycom13g20460 pycom16g19890
rosa_chinensis RchiOBHm_Chr2g0118961 RchiOBHm_Chr2g0148671 RchiOBHm_Chr2g0150531 RchiOBHm_Chr2g0171301 RchiOBHm_Chr3g0455021 RchiOBHm_Chr6g0249451
rosa_laevigata RLG00000001225 RLG00000001972 RLG00000008936 RLG00000009011 RLG00000009032 RLG00000015372 RLG00000019992 RLG00000020680 RLG00000026543
rosa_multiflora Rmu_co8272705.1_g000001 Rmu_co8286409.1_g000001 Rmu_sc0000048.1_g000034 Rmu_sc0000824.1_g000002 Rmu_sc0001839.1_g000023 Rmu_sc0002070.1_g000050 Rmu_sc0002349.1_g000005 Rmu_sc0002453.1_g000019 Rmu_sc0006431.1_g000026 Rmu_sc0006758.1_g000001 Rmu_sc0006758.1_g000013 Rmu_sc0006997.1_g000015 Rmu_sc0008411.1_g000007 Rmu_sc0008411.1_g000019 Rmu_sc0008411.1_g000028 Rmu_sc0008678.1_g000001
rosa_roxburghii Rroxscaffold_3G00225850 Rroxscaffold_3G00225860 Rroxscaffold_3G00233460 Rroxscaffold_3G00234260 Rroxscaffold_3G00236880 Rroxscaffold_3G00236890 Rroxscaffold_4G00294300 Rroxscaffold_4G00297380 Rroxscaffold_4G00324650 Rroxscaffold_5G00352840 Rroxscaffold_5G00354640 Rroxscaffold_5G00361500 Rroxscaffold_7G00156410 Rroxscaffold_7G00156420 Rroxscaffold_7G00198110 Rroxscaffold_7G00198120
rosa_samantha Rh1CG014400 Rh1DG009900 Rh2AG465900 Rh2BG342900 Rh2CG452400 Rh2DG488000 Rh3DG303300 Rh4AG118000 Rh4DG159500 Rh5AG246100 Rh6AG232100 Rh6BG178700 Rh6DG174100 Rh7BG231200 Rh7DG380300
rosa_wichuraiana Rw2G027640 Rw2G038070 Rw6G005920 Rw7G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AciI CCGC 1 cut(s) 158
AcsI RAATTY 1 cut(s) 17
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 101, 319, 362
AgsI TTSAA 2 cut(s) 101, 268
Alw26I GTCTC 2 cut(s) 152, 168
ApeKI GCWGC 3 cut(s) 197, 293, 366
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 1 cut(s) 6
AsuHPI GGTGA 1 cut(s) 245
BanII GRGCYC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 256
BbvI GCAGC 3 cut(s) 209, 305, 353
BccI CCATC 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 152, 168
BfaI CTAG 1 cut(s) 119
BisI GCNGC 3 cut(s) 198, 294, 367
BlsI GCNGC 3 cut(s) 199, 295, 368
BmiI GGNNCC 1 cut(s) 348
BpiI GAAGAC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 279
BsaI GGTCTC 2 cut(s) 152, 168
BsaJI CCNNGG 2 cut(s) 7, 360
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 319, 362
Bse1I ACTGG 2 cut(s) 228, 262
Bse3DI GCAATG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 7, 360
BseLI CCNNNNNNNGG 3 cut(s) 101, 319, 362
BseMI GCAATG 1 cut(s) 369
BseNI ACTGG 2 cut(s) 228, 262
BseXI GCAGC 3 cut(s) 209, 305, 353
BseYI CCCAGC 1 cut(s) 243
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 3 cut(s) 101, 319, 362
BsmAI GTCTC 2 cut(s) 152, 168
BsmFI GGGAC 1 cut(s) 174
Bso31I GGTCTC 2 cut(s) 152, 168
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 1 cut(s) 110
Bsp19I CCATGG 1 cut(s) 7
BspACI CCGC 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 348
BspTNI GGTCTC 2 cut(s) 152, 168
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 2 cut(s) 228, 262
BssECI CCNNGG 2 cut(s) 7, 360
BssMI GATC 1 cut(s) 110
BssT1I CCWWGG 2 cut(s) 7, 360
Bst4CI ACNGT 3 cut(s) 47, 318, 380
Bst6I CTCTTC 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 281
BstDSI CCRYGG 1 cut(s) 7
BstENI CCTNNNNNAGG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 113
BstMAI GTCTC 2 cut(s) 152, 168
BstMBI GATC 1 cut(s) 110
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 283
BstV1I GCAGC 3 cut(s) 209, 305, 353
BstV2I GAAGAC 1 cut(s) 256
BtgI CCRYGG 1 cut(s) 7
BtsIMutI CAGTG 2 cut(s) 255, 314
Cac8I GCNNGC 1 cut(s) 281
CfoI GCGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 122
CviAII CATG 6 cut(s) 8, 31, 72, 155, 194, 280
CviJI RGCY 3 cut(s) 137, 323, 366
CviKI_1 RGCY 3 cut(s) 137, 323, 366
CviQI GTAC 1 cut(s) 122
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 7, 360
Eco24I GRGCYC 1 cut(s) 325
Eco31I GGTCTC 2 cut(s) 152, 168
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoT14I CCWWGG 2 cut(s) 7, 360
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 7, 360
FaeI CATG 6 cut(s) 11, 34, 75, 158, 197, 283
FaqI GGGAC 1 cut(s) 174
FatI CATG 6 cut(s) 7, 30, 71, 154, 193, 279
FauI CCCGC 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 198, 294, 367
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 3 cut(s) 198, 294, 367
FspBI CTAG 1 cut(s) 119
GlaI GCGC 1 cut(s) 5
GluI GCNGC 3 cut(s) 198, 294, 367
GsaI CCCAGC 1 cut(s) 247
GsuI CTGGAG 1 cut(s) 279
HhaI GCGC 1 cut(s) 6
Hin1II CATG 6 cut(s) 11, 34, 75, 158, 197, 283
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 1 cut(s) 261
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 77
Hpy188III TCNNGA 2 cut(s) 98, 268
Hpy8I GTNNAC 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 95, 366
HpyCH4III ACNGT 3 cut(s) 47, 318, 380
HpyCH4V TGCA 6 cut(s) 34, 197, 279, 283, 293, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
Hsp92II CATG 6 cut(s) 11, 34, 75, 158, 197, 283
HspAI GCGC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 110
LpnPI CCDG 6 cut(s) 149, 209, 233, 243, 257, 311
Lsp1109I GCAGC 3 cut(s) 209, 305, 353
MaeI CTAG 1 cut(s) 119
MalI GATC 1 cut(s) 112
MboI GATC 1 cut(s) 110
MboII GAAGA 3 cut(s) 179, 256, 283
MhlI GDGCHC 1 cut(s) 325
MluCI AATT 2 cut(s) 17, 214
MlyI GAGTC 1 cut(s) 270
MnlI CCTC 2 cut(s) 105, 195
MwoI GCNNNNNNNGC 1 cut(s) 164
NcoI CCATGG 1 cut(s) 7
NdeII GATC 1 cut(s) 110
NlaIII CATG 6 cut(s) 11, 34, 75, 158, 197, 283
NlaIV GGNNCC 1 cut(s) 348
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PkrI GCNGC 3 cut(s) 199, 295, 368
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
PsiI TTATAA 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 348
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
SatI GCNGC 3 cut(s) 198, 294, 367
Sau3AI GATC 1 cut(s) 110
SchI GAGTC 1 cut(s) 270
SduI GDGCHC 1 cut(s) 325
SetI ASST 1 cut(s) 187
SphI GCATGC 1 cut(s) 283
Sse9I AATT 2 cut(s) 17, 214
SsiI CCGC 1 cut(s) 158
SspI AATATT 1 cut(s) 335
SspMI CTAG 1 cut(s) 119
StyI CCWWGG 2 cut(s) 7, 360
TaaI ACNGT 3 cut(s) 47, 318, 380
TasI AATT 2 cut(s) 17, 214
TatI WGTACW 1 cut(s) 121
TscAI CASTG 2 cut(s) 262, 321
TseI GCWGC 3 cut(s) 197, 293, 366
TspDTI ATGAA 3 cut(s) 60, 143, 179
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 2 cut(s) 262, 321
XagI CCTNNNNNAGG 1 cut(s) 99
XapI RAATTY 1 cut(s) 17
XceI RCATGY 1 cut(s) 283
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.