Rroxscaffold_3G00225860

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000689
Physical Location & Seq
Reverse (-)
9181613 .. 9197350
15738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225860.1

Sequence Viewer

Length: 837 bp
ATGAAGTTAAGTAATGGTGGATCTGCATGTTGCATGCAGCAACATCAAGTAAATTGGGAGGGGAGGTTCTACGCAGTTATAGAGAGAACAAATTTGAACCCAAGTGAAGTAGGGGATATAGTGGTTGGCACAGTTCTCGCTCCCGGCTCACTTAGAGCAATTGAGTGTAGGATGGCAGCATTTTATATTGTGGACCCAAAACCTGGAGAGGAGAGGCATGTTACAATTTATGTGGATGATGGCATCCGGCCAAATGCAAACATGACTGATTTGGCGAAATTGAAGCTTCCATTTAAATCGATGGGACAACAACTGCAGGGGACTACTAAAATGTGTCGTCACAAACTGCAAGTTACAACAAAAGCACACCGAAATGTGTGGTTGCAGTTGCACGGAGTCTGCTTCACCTATGCTGGGTGGTTTGGAATAAAGGGTTTGGTTGTTGCTGGAAGGACTGATGAAGACTGCTCTAGCATCCGTAAAGCATGTGATTTTTTGTTATCCAGAGAGTTTGCTTCTAGTGGATGGGGAGAAAGTTATCTATCATGTCAGAACAAGGTGTATTCAAATCTTCAGGATAATAGGCCACATATAGTCAATACTGCATGGGCTATGTTGGCCCTACTTGGTGCTGGGCAGGCAAAGAGAGACCCAACTCCATTGCACCGTGCAGCTAGGGTACTATTAAATTGTCAAATGGCAAATGGAGATTTTCCCCAGAAGGAGATCATGGGAGTTTTTAATAAGAACTGTATGATTAGCTATTCAGCATACCGAAACATTTTCCCCGTTTGGGCCCTTGGAGAATGTCGTTGTCAAGTATTCGCGGCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

278

Amino Acids

31.0

Weight (kDa)

9.0

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 120 - 271 2.6e-21 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000689)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08040
malus_domestica MD09G1245800.v1.1 MD11G1098500.v1.1 MD16G1237300.v1.1
pyrus_communis pycom03g07090 pycom03g07100 pycom09g08560 pycom13g20460 pycom16g19890
rosa_chinensis RchiOBHm_Chr2g0118961 RchiOBHm_Chr2g0148671 RchiOBHm_Chr2g0150531 RchiOBHm_Chr2g0171301 RchiOBHm_Chr3g0455021 RchiOBHm_Chr6g0249451
rosa_laevigata RLG00000001225 RLG00000001972 RLG00000008936 RLG00000009011 RLG00000009032 RLG00000015372 RLG00000019992 RLG00000020680 RLG00000026543
rosa_multiflora Rmu_co8272705.1_g000001 Rmu_co8286409.1_g000001 Rmu_sc0000048.1_g000034 Rmu_sc0000824.1_g000002 Rmu_sc0001839.1_g000023 Rmu_sc0002070.1_g000050 Rmu_sc0002349.1_g000005 Rmu_sc0002453.1_g000019 Rmu_sc0006431.1_g000026 Rmu_sc0006758.1_g000001 Rmu_sc0006758.1_g000013 Rmu_sc0006997.1_g000015 Rmu_sc0008411.1_g000007 Rmu_sc0008411.1_g000019 Rmu_sc0008411.1_g000028 Rmu_sc0008678.1_g000001
rosa_roxburghii Rroxscaffold_3G00225850 Rroxscaffold_3G00225860 Rroxscaffold_3G00233460 Rroxscaffold_3G00234260 Rroxscaffold_3G00236880 Rroxscaffold_3G00236890 Rroxscaffold_4G00294300 Rroxscaffold_4G00297380 Rroxscaffold_4G00324650 Rroxscaffold_5G00352840 Rroxscaffold_5G00354640 Rroxscaffold_5G00361500 Rroxscaffold_7G00156410 Rroxscaffold_7G00156420 Rroxscaffold_7G00198110 Rroxscaffold_7G00198120
rosa_samantha Rh1CG014400 Rh1DG009900 Rh2AG465900 Rh2BG342900 Rh2CG452400 Rh2DG488000 Rh3DG303300 Rh4AG118000 Rh4DG159500 Rh5AG246100 Rh6AG232100 Rh6BG178700 Rh6DG174100 Rh7BG231200 Rh7DG380300
rosa_wichuraiana Rw2G027640 Rw2G038070 Rw6G005920 Rw7G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 203
AccII CGCG 1 cut(s) 827
AciI CCGC 1 cut(s) 827
AclWI GGATC 1 cut(s) 28
AcoI YGGCCR 1 cut(s) 248
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 557
AfaI GTAC 1 cut(s) 681
AfiI CCNNNNNNNGG 3 cut(s) 203, 414, 793
AgsI TTSAA 3 cut(s) 97, 283, 567
AjnI CCWGG 1 cut(s) 202
AloI GAACNNNNNNTCC 2 cut(s) 50, 82
AluBI AGCT 3 cut(s) 286, 674, 762
AluI AGCT 3 cut(s) 286, 674, 762
Alw26I GTCTC 1 cut(s) 642
AlwI GGATC 1 cut(s) 28
AoxI GGCC 5 cut(s) 248, 584, 618, 795, 828
ApaI GGGCCC 1 cut(s) 799
ApeKI GCWGC 3 cut(s) 37, 176, 671
ApoI RAATTY 1 cut(s) 91
AspS9I GGNCC 5 cut(s) 193, 619, 795, 796, 829
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 397
AvaII GGWCC 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 799
BanII GRGCYC 1 cut(s) 799
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 3 cut(s) 49, 188, 683
BccI CCATC 4 cut(s) 166, 233, 295, 519
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 204
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 642
BfaI CTAG 3 cut(s) 471, 519, 675
BfmI CTRYAG 1 cut(s) 314
BisI GCNGC 4 cut(s) 38, 177, 672, 828
BlsI GCNGC 4 cut(s) 39, 178, 673, 829
Bme1390I CCNGG 2 cut(s) 144, 204
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 5 cut(s) 193, 619, 795, 796, 829
BmiI GGNNCC 2 cut(s) 195, 797
BmrFI CCNGG 2 cut(s) 144, 204
BmsI GCATC 2 cut(s) 252, 483
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 144
Bsa29I ATCGAT 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 642
BsaJI CCNNGG 1 cut(s) 799
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 414, 793
Bse3DI GCAATG 1 cut(s) 659
BseBI CCWGG 1 cut(s) 204
BseCI ATCGAT 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 799
BseGI GGATG 5 cut(s) 177, 241, 243, 474, 530
BseLI CCNNNNNNNGG 3 cut(s) 203, 414, 793
BseMI GCAATG 1 cut(s) 659
BseRI GAGGAG 1 cut(s) 224
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 3 cut(s) 49, 188, 683
BseYI CCCAGC 2 cut(s) 413, 632
BsgI GTGCAG 1 cut(s) 690
Bsh1236I CGCG 1 cut(s) 827
BshFI GGCC 5 cut(s) 250, 586, 620, 797, 830
BshVI ATCGAT 1 cut(s) 299
BsiSI CCGG 2 cut(s) 144, 247
BslFI GGGAC 2 cut(s) 318, 334
BslI CCNNNNNNNGG 3 cut(s) 203, 414, 793
BsmAI GTCTC 1 cut(s) 642
BsmFI GGGAC 2 cut(s) 318, 334
BsnI GGCC 5 cut(s) 250, 586, 620, 797, 830
Bso31I GGTCTC 1 cut(s) 642
Bsp120I GGGCCC 1 cut(s) 795
Bsp1286I GDGCHC 1 cut(s) 799
Bsp143I GATC 2 cut(s) 20, 726
BspACI CCGC 1 cut(s) 827
BspANI GGCC 5 cut(s) 250, 586, 620, 797, 830
BspDI ATCGAT 1 cut(s) 299
BspFNI CGCG 1 cut(s) 827
BspLI GGNNCC 2 cut(s) 195, 797
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 659
BssECI CCNNGG 1 cut(s) 799
BssMI GATC 2 cut(s) 20, 726
BssT1I CCWWGG 1 cut(s) 799
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 3 cut(s) 133, 668, 752
BstC8I GCNNGC 2 cut(s) 35, 639
BstDEI CTNAG 1 cut(s) 152
BstF5I GGATG 5 cut(s) 177, 241, 243, 474, 530
BstFNI CGCG 1 cut(s) 827
BstKTI GATC 2 cut(s) 23, 729
BstMAI GTCTC 1 cut(s) 642
BstMBI GATC 2 cut(s) 20, 726
BstMWI GCNNNNNNNGC 2 cut(s) 617, 638
BstNI CCWGG 1 cut(s) 204
BstNSI RCATGY 4 cut(s) 30, 37, 221, 489
BstSCI CCNGG 2 cut(s) 142, 202
BstSFI CTRYAG 1 cut(s) 314
BstSLI GKGCMC 1 cut(s) 799
BstUI CGCG 1 cut(s) 827
BstV1I GCAGC 3 cut(s) 49, 188, 683
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
Bsu15I ATCGAT 1 cut(s) 299
BsuRI GGCC 5 cut(s) 250, 586, 620, 797, 830
BsuTUI ATCGAT 1 cut(s) 299
BtsCI GGATG 5 cut(s) 177, 241, 243, 474, 530
Cac8I GCNNGC 2 cut(s) 35, 639
Cfr13I GGNCC 5 cut(s) 193, 619, 795, 796, 829
ClaI ATCGAT 1 cut(s) 299
Csp6I GTAC 1 cut(s) 680
CspCI CAANNNNNGTGG 2 cut(s) 213, 248
CviAII CATG 8 cut(s) 27, 34, 218, 262, 486, 546, 606, 730
CviQI GTAC 1 cut(s) 680
DdeI CTNAG 1 cut(s) 152
DpnI GATC 2 cut(s) 22, 728
DpnII GATC 2 cut(s) 20, 726
DraI TTTAAA 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 799
Eco31I GGTCTC 1 cut(s) 642
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 796
EcoRII CCWGG 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 799
EcoT38I GRGCYC 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 8 cut(s) 30, 37, 221, 265, 489, 549, 609, 733
FaqI GGGAC 2 cut(s) 318, 334
FatI CATG 8 cut(s) 26, 33, 217, 261, 485, 545, 605, 729
Fnu4HI GCNGC 4 cut(s) 38, 177, 672, 828
FokI GGATG 5 cut(s) 184, 230, 248, 461, 537
FriOI GRGCYC 1 cut(s) 799
Fsp4HI GCNGC 4 cut(s) 38, 177, 672, 828
FspBI CTAG 3 cut(s) 471, 519, 675
GluI GCNGC 4 cut(s) 38, 177, 672, 828
GsaI CCCAGC 2 cut(s) 417, 636
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 5 cut(s) 250, 586, 620, 797, 830
HapII CCGG 2 cut(s) 144, 247
Hin1II CATG 8 cut(s) 30, 37, 221, 265, 489, 549, 609, 733
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 1 cut(s) 396
HpaII CCGG 2 cut(s) 144, 247
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 2 cut(s) 552, 836
Hpy188III TCNNGA 2 cut(s) 504, 575
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 2 cut(s) 444, 715
HpyCH4III ACNGT 3 cut(s) 133, 668, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 638
HpyF3I CTNAG 1 cut(s) 152
Hsp92II CATG 8 cut(s) 30, 37, 221, 265, 489, 549, 609, 733
Kzo9I GATC 2 cut(s) 20, 726
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 3 cut(s) 49, 188, 683
LweI GCATC 2 cut(s) 252, 483
MaeI CTAG 3 cut(s) 471, 519, 675
MaeIII GTNAC 3 cut(s) 220, 338, 352
MalI GATC 2 cut(s) 22, 728
MboI GATC 2 cut(s) 20, 726
MboII GAAGA 2 cut(s) 473, 563
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 1 cut(s) 799
MluCI AATT 6 cut(s) 52, 91, 159, 225, 278, 688
MlyI GAGTC 1 cut(s) 405
MnlI CCTC 4 cut(s) 52, 57, 202, 207
MseI TTAA 4 cut(s) 8, 294, 686, 741
MslI CAYNNNNRTG 1 cut(s) 372
MspI CCGG 2 cut(s) 144, 247
MspR9I CCNGG 2 cut(s) 144, 204
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 204
MvnI CGCG 1 cut(s) 827
MwoI GCNNNNNNNGC 2 cut(s) 617, 638
NciI CCSGG 1 cut(s) 144
NdeII GATC 2 cut(s) 20, 726
NlaIII CATG 8 cut(s) 30, 37, 221, 265, 489, 549, 609, 733
NlaIV GGNNCC 2 cut(s) 195, 797
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 4 cut(s) 30, 37, 221, 489
PaeI GCATGC 1 cut(s) 37
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 4 cut(s) 39, 178, 673, 829
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 202
PspFI CCCAGC 2 cut(s) 413, 632
PspGI CCWGG 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 195, 797
PspOMI GGGCCC 1 cut(s) 795
PspPI GGNCC 5 cut(s) 193, 619, 795, 796, 829
PstI CTGCAG 1 cut(s) 318
PsuI RGATCY 1 cut(s) 20
RsaI GTAC 1 cut(s) 681
RsaNI GTAC 1 cut(s) 680
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 4 cut(s) 8, 294, 686, 741
SatI GCNGC 4 cut(s) 38, 177, 672, 828
Sau3AI GATC 2 cut(s) 20, 726
Sau96I GGNCC 5 cut(s) 193, 619, 795, 796, 829
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 2 cut(s) 144, 204
SduI GDGCHC 1 cut(s) 799
SetI ASST 7 cut(s) 68, 205, 288, 410, 561, 676, 764
SfaNI GCATC 2 cut(s) 252, 483
SfcI CTRYAG 1 cut(s) 314
SinI GGWCC 1 cut(s) 193
SmiI ATTTAAAT 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 372
SphI GCATGC 1 cut(s) 37
Sse9I AATT 6 cut(s) 52, 91, 159, 225, 278, 688
SsiI CCGC 1 cut(s) 827
SspMI CTAG 3 cut(s) 471, 519, 675
StyD4I CCNGG 2 cut(s) 142, 202
StyI CCWWGG 1 cut(s) 799
SwaI ATTTAAAT 1 cut(s) 295
TaaI ACNGT 3 cut(s) 133, 668, 752
TaqI TCGA 1 cut(s) 299
TasI AATT 6 cut(s) 52, 91, 159, 225, 278, 688
TauI GCSGC 1 cut(s) 830
Tru1I TTAA 4 cut(s) 8, 294, 686, 741
Tru9I TTAA 4 cut(s) 8, 294, 686, 741
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 3 cut(s) 37, 176, 671
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 2 cut(s) 17, 474
TspGWI ACGGA 2 cut(s) 408, 467
Van91I CCANNNNNTGG 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 91
XceI RCATGY 4 cut(s) 30, 37, 221, 489
XspI CTAG 3 cut(s) 471, 519, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.