RLG00000015372

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000689
Physical Location & Seq
Forward (+)
65173952 .. 65175210
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015372

Sequence Viewer

Length: 411 bp
ATGATCAAAATCTTCTATTACTTTCCAGGTATTACTGCTGCTTCTGGGATAGCAGCAGAGCTGGGGATCTCATTAACGCACAGAGGTGTTGCAAATAGTCTGTTAAATCCGACAGAGTTCTTGGAGGACATTGTAATTGAGCATGAACATGTTGAGTGCACTTCATCTGTAATCCAGGCTTTAGTTCTGTTCAAGAAGCTACACCCTGGGCATAGGAAGAAAGAGATTGAACATTCCATCACCAATGCTGTACGCTACATTGAAAATATGCAAATGCCGAATGGTTCATGGTATGGAGATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCACCAGGAGGATTGGCAGCTGCTGGCAAGACTTTTAGCACTTGTGCAGCCATGCGCAATGGGGTTAGCAGGAGATGA

Protein Analysis

137

Amino Acids

15.02

Weight (kDa)

7.78

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 72 - 125 2.3e-11 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000689)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08040
malus_domestica MD09G1245800.v1.1 MD11G1098500.v1.1 MD16G1237300.v1.1
pyrus_communis pycom03g07090 pycom03g07100 pycom09g08560 pycom13g20460 pycom16g19890
rosa_chinensis RchiOBHm_Chr2g0118961 RchiOBHm_Chr2g0148671 RchiOBHm_Chr2g0150531 RchiOBHm_Chr2g0171301 RchiOBHm_Chr3g0455021 RchiOBHm_Chr6g0249451
rosa_laevigata RLG00000001225 RLG00000001972 RLG00000008936 RLG00000009011 RLG00000009032 RLG00000015372 RLG00000019992 RLG00000020680 RLG00000026543
rosa_multiflora Rmu_co8272705.1_g000001 Rmu_co8286409.1_g000001 Rmu_sc0000048.1_g000034 Rmu_sc0000824.1_g000002 Rmu_sc0001839.1_g000023 Rmu_sc0002070.1_g000050 Rmu_sc0002349.1_g000005 Rmu_sc0002453.1_g000019 Rmu_sc0006431.1_g000026 Rmu_sc0006758.1_g000001 Rmu_sc0006758.1_g000013 Rmu_sc0006997.1_g000015 Rmu_sc0008411.1_g000007 Rmu_sc0008411.1_g000019 Rmu_sc0008411.1_g000028 Rmu_sc0008678.1_g000001
rosa_roxburghii Rroxscaffold_3G00225850 Rroxscaffold_3G00225860 Rroxscaffold_3G00233460 Rroxscaffold_3G00234260 Rroxscaffold_3G00236880 Rroxscaffold_3G00236890 Rroxscaffold_4G00294300 Rroxscaffold_4G00297380 Rroxscaffold_4G00324650 Rroxscaffold_5G00352840 Rroxscaffold_5G00354640 Rroxscaffold_5G00361500 Rroxscaffold_7G00156410 Rroxscaffold_7G00156420 Rroxscaffold_7G00198110 Rroxscaffold_7G00198120
rosa_samantha Rh1CG014400 Rh1DG009900 Rh2AG465900 Rh2BG342900 Rh2CG452400 Rh2DG488000 Rh3DG303300 Rh4AG118000 Rh4DG159500 Rh5AG246100 Rh6AG232100 Rh6BG178700 Rh6DG174100 Rh7BG231200 Rh7DG380300
rosa_wichuraiana Rw2G027640 Rw2G038070 Rw6G005920 Rw7G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 389
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 323
AclWI GGATC 1 cut(s) 74
AfaI GTAC 2 cut(s) 252, 325
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 3 cut(s) 193, 230, 263
AjnI CCWGG 5 cut(s) 25, 174, 205, 326, 337
AleI CACNNNNGTG 1 cut(s) 84
AluBI AGCT 3 cut(s) 61, 199, 353
AluI AGCT 3 cut(s) 61, 199, 353
Alw21I GWGCWC 1 cut(s) 161
Alw44I GTGCAC 1 cut(s) 157
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 356
ApaLI GTGCAC 1 cut(s) 157
ApeKI GCWGC 5 cut(s) 38, 53, 350, 353, 380
Asp718I GGTACC 1 cut(s) 323
AspLEI GCGC 1 cut(s) 390
AsuHPI GGTGA 1 cut(s) 232
BaeGI GKGCMC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 323
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 5 cut(s) 25, 65, 340, 362, 392
BccI CCATC 1 cut(s) 245
BciT130I CCWGG 5 cut(s) 27, 176, 207, 328, 339
BclI TGATCA 1 cut(s) 3
BisI GCNGC 5 cut(s) 39, 54, 351, 354, 381
BlsI GCNGC 5 cut(s) 40, 55, 352, 355, 382
Bme1390I CCNGG 5 cut(s) 27, 176, 207, 328, 339
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 5 cut(s) 27, 176, 207, 328, 339
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 205, 206
Bse3DI GCAATG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 5 cut(s) 27, 176, 207, 328, 339
BseDI CCNNGG 2 cut(s) 205, 206
BseJI GATNNNNATC 1 cut(s) 8
BseMI GCAATG 1 cut(s) 397
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 5 cut(s) 25, 65, 340, 362, 392
BseYI CCCAGC 1 cut(s) 61
BsgI GTGCAG 1 cut(s) 399
BshNI GGYRCC 1 cut(s) 323
BsiHKAI GWGCWC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 161
Bsp143I GATC 2 cut(s) 3, 66
BspLI GGNNCC 1 cut(s) 325
BspPI GGATC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 397
BssECI CCNNGG 2 cut(s) 205, 206
BssMI GATC 2 cut(s) 3, 66
Bst2UI CCWGG 5 cut(s) 27, 176, 207, 328, 339
BstC8I GCNNGC 1 cut(s) 358
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 2 cut(s) 6, 69
BstMBI GATC 2 cut(s) 3, 66
BstNI CCWGG 5 cut(s) 27, 176, 207, 328, 339
BstNSI RCATGY 1 cut(s) 152
BstSCI CCNGG 5 cut(s) 25, 174, 205, 326, 337
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 5 cut(s) 25, 65, 340, 362, 392
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
Cac8I GCNNGC 1 cut(s) 358
CaiI CAGNNNCTG 1 cut(s) 356
CfoI GCGC 1 cut(s) 390
CsiI ACCWGGT 1 cut(s) 326
Csp6I GTAC 2 cut(s) 251, 324
CviAII CATG 4 cut(s) 143, 149, 288, 385
CviJI RGCY 5 cut(s) 61, 179, 199, 353, 383
CviKI_1 RGCY 5 cut(s) 61, 179, 199, 353, 383
CviQI GTAC 2 cut(s) 251, 324
DpnI GATC 2 cut(s) 5, 68
DpnII GATC 2 cut(s) 3, 66
EcoRII CCWGG 5 cut(s) 25, 174, 205, 326, 337
FaeI CATG 4 cut(s) 146, 152, 291, 388
FaiI YATR 9 cut(s) 144, 150, 213, 269, 289, 294, 319, 321, 386
FatI CATG 4 cut(s) 142, 148, 287, 384
FauNDI CATATG 1 cut(s) 319
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 5 cut(s) 39, 54, 351, 354, 381
Fsp4HI GCNGC 5 cut(s) 39, 54, 351, 354, 381
FspI TGCGCA 1 cut(s) 389
GlaI GCGC 1 cut(s) 389
GluI GCNGC 5 cut(s) 39, 54, 351, 354, 381
GsaI CCCAGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 390
Hin1II CATG 4 cut(s) 146, 152, 291, 388
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 1 cut(s) 159
HpyCH4V TGCA 5 cut(s) 92, 159, 271, 335, 380
Hsp92II CATG 4 cut(s) 146, 152, 291, 388
HspAI GCGC 1 cut(s) 388
KpnI GGTACC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 66
Lsp1109I GCAGC 5 cut(s) 25, 65, 340, 362, 392
MabI ACCWGGT 1 cut(s) 326
MalI GATC 2 cut(s) 5, 68
MboI GATC 2 cut(s) 3, 66
MboII GAAGA 2 cut(s) 4, 229
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 1 cut(s) 135
MmeI TCCRAC 1 cut(s) 134
MnlI CCTC 3 cut(s) 77, 118, 335
MseI TTAA 2 cut(s) 74, 104
MslI CAYNNNNRTG 2 cut(s) 84, 147
MspA1I CMGCKG 1 cut(s) 353
MspR9I CCNGG 5 cut(s) 27, 176, 207, 328, 339
MvaI CCWGG 5 cut(s) 27, 176, 207, 328, 339
NdeI CATATG 1 cut(s) 319
NdeII GATC 2 cut(s) 3, 66
NlaIII CATG 4 cut(s) 146, 152, 291, 388
NlaIV GGNNCC 1 cut(s) 325
NsbI TGCGCA 1 cut(s) 389
NspI RCATGY 1 cut(s) 152
OliI CACNNNNGTG 1 cut(s) 84
PasI CCCWGGG 1 cut(s) 206
PciI ACATGT 1 cut(s) 148
PkrI GCNGC 5 cut(s) 40, 55, 352, 355, 382
PscI ACATGT 1 cut(s) 148
Psp6I CCWGG 5 cut(s) 25, 174, 205, 326, 337
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 5 cut(s) 25, 174, 205, 326, 337
PspN4I GGNNCC 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 2 cut(s) 252, 325
RsaNI GTAC 2 cut(s) 251, 324
RseI CAYNNNNRTG 2 cut(s) 84, 147
SaqAI TTAA 2 cut(s) 74, 104
SatI GCNGC 5 cut(s) 39, 54, 351, 354, 381
Sau3AI GATC 2 cut(s) 3, 66
ScrFI CCNGG 5 cut(s) 27, 176, 207, 328, 339
SduI GDGCHC 1 cut(s) 161
SetI ASST 6 cut(s) 31, 63, 88, 201, 329, 355
SexAI ACCWGGT 1 cut(s) 326
SmiMI CAYNNNNRTG 2 cut(s) 84, 147
Sse9I AATT 1 cut(s) 135
StyD4I CCNGG 5 cut(s) 25, 174, 205, 326, 337
TasI AATT 1 cut(s) 135
Tru1I TTAA 2 cut(s) 74, 104
Tru9I TTAA 2 cut(s) 74, 104
TseI GCWGC 5 cut(s) 38, 53, 350, 353, 380
TspDTI ATGAA 3 cut(s) 153, 159, 276
VneI GTGCAC 1 cut(s) 157
XceI RCATGY 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.