RLG00000020680

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000689
Physical Location & Seq
Forward (+)
67534970 .. 67552592
17623 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020680

Sequence Viewer

Length: 1671 bp
ATGATCAAAATCTTCTATTACTTTCCAGGTATTACTGCTGCTTCTGGGATAGCAGCAGAGCTGGGGATCCCATTAACGCACAGAGGTGTTGCAAATAGTCTGTTAAATCCGACAGAGTTCTTGGAGGACATTGTTATTGAGCATGAACATGTTGAGTGCACTTCATCTGTAATCCAGGCTTTAGTTCTGTTCAAGAAGCTACACCCTGGGCATAGGAAGAAAGAGATTGAACATTCCATCACCAATGTTGTACGCTACATTGAAAATATGCAAATGCCGGATGGTTCATGGTATGGAGATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCACTAGGAGGATTGGCAGCTGCTGGCAAGACTTTTAGCACTTGTGCAGCCATGCGCAATGGGGGGGAGAGGTTTGGACTGGAAACAAAGAAAACGACGAATCTTGGTATTATTGCTGGTGTGGTTGCTCTGGTTTTTGGATTCAATGAGTTGCCTGAAGTGGGTTGGAGGGTGAAGAGGATCTCATCACTGAACGGTATTACTGCTGCTTCTGGGATAGCAGCAGAGCTGGGGATCCCATTAACGCACAGAGGTGTTGCAAATAGTCTGTTAAATCCGACAGAGTTCTTGGAGGACATTGTTATTGAGCATGAACATGTTGAGTGCACTTCATCTGTAATCCAGGCTTTAGTTCTGTTCAAGAAGCTACACCCTGGGCATAGGAAGAAAGAGATTGAACATTCCATCACCAATGTTGTACGCTACATTGAAAATATGCAAATGCCGGATGGTTCATGGTATGGAGATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCACTAGGAGGATTGGCAGCTGCTGGCAAGACTTTTAGCACTTGTGCAGCCATGCGCAATGGGGGGGAGAGGTTTGGACTGGAAACAAAGAAAACGACGAATCTTGGTATTATTGCTGGTGTGGTTGCTCTGGTTTTTGGATTCAATGAGTTGCCTGAAGTGGGTTGGAGGGTGAAGAGGATCTCATCACTGAACGTAAAAGTGAAGATGTTGATCGGGTGCCTGATTCCAAATTCTAACAAGACTGATCGGGCTTCAATGCTGGATGAGGCAATTGATCGCTTTGAAAGCACCTGTGTACTCAATTGGACCAACTTAGGAGTGGAGTGCCTTCTGTGTAATAAAAAGTTTCTGGTCAAGATTGTGCATATTGAAGGGGGACAGTTTCAATCCTTTGTTTTGATTGCATTACTCTGTAAAAATTTCAAAGATGAGACTATTGTTGACATCGATGATGAGGAGGAGAGGAGAGGGGAAGATACTTTTGCCCTCATCCCTGACCAGAGGAGAATTTGGATTCATTCGCTGTCAAAACATTTAGTTCCTCCAATTCAGAATTCAGAGAAAGTTGATTTCACAAACCTTATATTTTCCAAACATTGTAAATGTTCCTCTCTTCAAGGGATGCTCAATGGAATTCATCAATCAGATTACCCTGAAGACCAGATCTGCCTATTCGGAGTCCAGAAGCGCATACATTATGTCTTTCACAATCTCAGACTCACCGATCTCAATCAATCTCCGTCAAGACTTGGTCGAAAAGAGGAAATCCGTCGTTCTCACTCAAAAACAAAGGTTGGACTAGCCAGAAGTTTTTTAAGGATGGATTGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 ko01110 map00909 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

557

Amino Acids

62.09

Weight (kDa)

7.88

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 73 - 124 3.8e-11 Squalene-hopene cyclase C-terminal domain
SQHop_cyclase_C PF13243 240 - 291 3.8e-11 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000689)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08040
malus_domestica MD09G1245800.v1.1 MD11G1098500.v1.1 MD16G1237300.v1.1
pyrus_communis pycom03g07090 pycom03g07100 pycom09g08560 pycom13g20460 pycom16g19890
rosa_chinensis RchiOBHm_Chr2g0118961 RchiOBHm_Chr2g0148671 RchiOBHm_Chr2g0150531 RchiOBHm_Chr2g0171301 RchiOBHm_Chr3g0455021 RchiOBHm_Chr6g0249451
rosa_laevigata RLG00000001225 RLG00000001972 RLG00000008936 RLG00000009011 RLG00000009032 RLG00000015372 RLG00000019992 RLG00000020680 RLG00000026543
rosa_multiflora Rmu_co8272705.1_g000001 Rmu_co8286409.1_g000001 Rmu_sc0000048.1_g000034 Rmu_sc0000824.1_g000002 Rmu_sc0001839.1_g000023 Rmu_sc0002070.1_g000050 Rmu_sc0002349.1_g000005 Rmu_sc0002453.1_g000019 Rmu_sc0006431.1_g000026 Rmu_sc0006758.1_g000001 Rmu_sc0006758.1_g000013 Rmu_sc0006997.1_g000015 Rmu_sc0008411.1_g000007 Rmu_sc0008411.1_g000019 Rmu_sc0008411.1_g000028 Rmu_sc0008678.1_g000001
rosa_roxburghii Rroxscaffold_3G00225850 Rroxscaffold_3G00225860 Rroxscaffold_3G00233460 Rroxscaffold_3G00234260 Rroxscaffold_3G00236880 Rroxscaffold_3G00236890 Rroxscaffold_4G00294300 Rroxscaffold_4G00297380 Rroxscaffold_4G00324650 Rroxscaffold_5G00352840 Rroxscaffold_5G00354640 Rroxscaffold_5G00361500 Rroxscaffold_7G00156410 Rroxscaffold_7G00156420 Rroxscaffold_7G00198110 Rroxscaffold_7G00198120
rosa_samantha Rh1CG014400 Rh1DG009900 Rh2AG465900 Rh2BG342900 Rh2CG452400 Rh2DG488000 Rh3DG303300 Rh4AG118000 Rh4DG159500 Rh5AG246100 Rh6AG232100 Rh6BG178700 Rh6DG174100 Rh7BG231200 Rh7DG380300
rosa_wichuraiana Rw2G027640 Rw2G038070 Rw6G005920 Rw7G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 389, 890
Acc65I GGTACC 2 cut(s) 323, 824
AccB1I GGYRCC 3 cut(s) 323, 824, 1053
AclWI GGATC 6 cut(s) 61, 74, 521, 562, 575, 1022
AcsI RAATTY 5 cut(s) 1066, 1255, 1344, 1390, 1470
AcuI CTGAAG 3 cut(s) 510, 1011, 1512
AfaI GTAC 5 cut(s) 252, 325, 753, 826, 1134
AfiI CCNNNNNNNGG 2 cut(s) 494, 995
AflIII ACRYGT 2 cut(s) 148, 649
AjnI CCWGG 7 cut(s) 25, 174, 205, 326, 675, 706, 827
AjuI GAANNNNNNNTTGG 2 cut(s) 1375, 1407
AleI CACNNNNGTG 2 cut(s) 84, 585
AluBI AGCT 6 cut(s) 61, 199, 353, 562, 700, 854
AluI AGCT 6 cut(s) 61, 199, 353, 562, 700, 854
Alw21I GWGCWC 2 cut(s) 161, 662
Alw26I GTCTC 1 cut(s) 1262
Alw44I GTGCAC 2 cut(s) 157, 658
AlwI GGATC 6 cut(s) 61, 74, 521, 562, 575, 1022
AlwNI CAGNNNCTG 2 cut(s) 356, 857
ApaLI GTGCAC 2 cut(s) 157, 658
ApoI RAATTY 5 cut(s) 1066, 1255, 1344, 1390, 1470
Asp718I GGTACC 2 cut(s) 323, 824
AspLEI GCGC 3 cut(s) 390, 891, 1527
AspS9I GGNCC 1 cut(s) 1143
AsuHPI GGTGA 5 cut(s) 232, 517, 733, 1018, 1549
AvaII GGWCC 1 cut(s) 1143
BaeGI GKGCMC 2 cut(s) 161, 662
BamHI GGATCC 2 cut(s) 66, 567
BanI GGYRCC 3 cut(s) 323, 824, 1053
BbsI GAAGAC 1 cut(s) 1500
Bbv12I GWGCWC 2 cut(s) 161, 662
BccI CCATC 5 cut(s) 245, 275, 746, 776, 1651
BciT130I CCWGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 1262
BfaI CTAG 3 cut(s) 338, 839, 1637
BglII AGATCT 1 cut(s) 1500
Bme1390I CCNGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
Bme18I GGWCC 1 cut(s) 1143
BmgT120I GGNCC 1 cut(s) 1143
BmiI GGNNCC 5 cut(s) 68, 325, 569, 826, 1055
BmrFI CCNGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
BmsI GCATC 1 cut(s) 1449
BpiI GAAGAC 1 cut(s) 1500
Bsa29I ATCGAT 1 cut(s) 1284
BsaBI GATNNNNATC 3 cut(s) 8, 1046, 1566
BsaJI CCNNGG 4 cut(s) 205, 206, 706, 707
Bsc4I CCNNNNNNNGG 2 cut(s) 494, 995
Bse1I ACTGG 2 cut(s) 417, 918
Bse3DI GCAATG 2 cut(s) 397, 898
Bse8I GATNNNNATC 3 cut(s) 8, 1046, 1566
BseBI CCWGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
BseCI ATCGAT 1 cut(s) 1284
BseDI CCNNGG 4 cut(s) 205, 206, 706, 707
BseGI GGATG 6 cut(s) 286, 787, 1105, 1326, 1464, 1662
BseJI GATNNNNATC 3 cut(s) 8, 1046, 1566
BseLI CCNNNNNNNGG 2 cut(s) 494, 995
BseMI GCAATG 2 cut(s) 397, 898
BseMII CTCAG 1 cut(s) 1564
BseNI ACTGG 2 cut(s) 417, 918
BseRI GAGGAG 4 cut(s) 1307, 1310, 1315, 1354
BseSI GKGCMC 2 cut(s) 161, 662
BseYI CCCAGC 2 cut(s) 61, 562
BsgI GTGCAG 2 cut(s) 399, 900
BshNI GGYRCC 3 cut(s) 323, 824, 1053
BshVI ATCGAT 1 cut(s) 1284
BsiHKAI GWGCWC 2 cut(s) 161, 662
BsiSI CCGG 2 cut(s) 278, 779
BslFI GGGAC 1 cut(s) 1227
BslI CCNNNNNNNGG 2 cut(s) 494, 995
BsmAI GTCTC 1 cut(s) 1262
BsmFI GGGAC 1 cut(s) 1227
Bsp1286I GDGCHC 2 cut(s) 161, 662
BspCNI CTCAG 1 cut(s) 1563
BspDI ATCGAT 1 cut(s) 1284
BspLI GGNNCC 5 cut(s) 68, 325, 569, 826, 1055
BspPI GGATC 6 cut(s) 61, 74, 521, 562, 575, 1022
BspT107I GGYRCC 3 cut(s) 323, 824, 1053
BsrDI GCAATG 2 cut(s) 397, 898
BsrI ACTGG 2 cut(s) 417, 918
BssECI CCNNGG 4 cut(s) 205, 206, 706, 707
Bst2UI CCWGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
Bst4CI ACNGT 2 cut(s) 530, 1218
Bst6I CTCTTC 3 cut(s) 503, 1004, 1455
BstC8I GCNNGC 2 cut(s) 358, 859
BstDEI CTNAG 2 cut(s) 1150, 1550
BstF5I GGATG 6 cut(s) 286, 787, 1105, 1326, 1464, 1662
BstHHI GCGC 3 cut(s) 390, 891, 1527
BstMAI GTCTC 1 cut(s) 1262
BstMWI GCNNNNNNNGC 1 cut(s) 1122
BstNI CCWGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
BstNSI RCATGY 2 cut(s) 152, 653
BstSCI CCNGG 7 cut(s) 25, 174, 205, 326, 675, 706, 827
BstSLI GKGCMC 2 cut(s) 161, 662
BstV2I GAAGAC 1 cut(s) 1500
BstX2I RGATCY 5 cut(s) 66, 513, 567, 1014, 1500
BstYI RGATCY 5 cut(s) 66, 513, 567, 1014, 1500
Bsu15I ATCGAT 1 cut(s) 1284
BsuTUI ATCGAT 1 cut(s) 1284
BtsCI GGATG 6 cut(s) 286, 787, 1105, 1326, 1464, 1662
BtsIMutI CAGTG 2 cut(s) 521, 1022
Cac8I GCNNGC 2 cut(s) 358, 859
CaiI CAGNNNCTG 2 cut(s) 356, 857
CfoI GCGC 3 cut(s) 390, 891, 1527
Cfr13I GGNCC 1 cut(s) 1143
ClaI ATCGAT 1 cut(s) 1284
CsiI ACCWGGT 2 cut(s) 326, 827
Csp6I GTAC 5 cut(s) 251, 324, 752, 825, 1133
CviAII CATG 8 cut(s) 143, 149, 288, 385, 644, 650, 789, 886
CviQI GTAC 5 cut(s) 251, 324, 752, 825, 1133
DdeI CTNAG 2 cut(s) 1150, 1550
Eam1104I CTCTTC 3 cut(s) 503, 1004, 1455
EarI CTCTTC 3 cut(s) 503, 1004, 1455
Eco47I GGWCC 1 cut(s) 1143
Eco57I CTGAAG 3 cut(s) 510, 1011, 1512
EcoRI GAATTC 2 cut(s) 1390, 1470
EcoRII CCWGG 7 cut(s) 25, 174, 205, 326, 675, 706, 827
FaeI CATG 8 cut(s) 146, 152, 291, 388, 647, 653, 792, 889
FaqI GGGAC 1 cut(s) 1227
FatI CATG 8 cut(s) 142, 148, 287, 384, 643, 649, 788, 885
FauNDI CATATG 2 cut(s) 319, 820
FbaI TGATCA 1 cut(s) 3
FokI GGATG 5 cut(s) 293, 794, 1112, 1313, 1471
FspBI CTAG 3 cut(s) 338, 839, 1637
FspI TGCGCA 2 cut(s) 389, 890
GlaI GCGC 3 cut(s) 389, 890, 1526
GsaI CCCAGC 2 cut(s) 65, 566
HapII CCGG 2 cut(s) 278, 779
HhaI GCGC 3 cut(s) 390, 891, 1527
Hin1II CATG 8 cut(s) 146, 152, 291, 388, 647, 653, 792, 889
Hin6I GCGC 3 cut(s) 388, 889, 1525
HinP1I GCGC 3 cut(s) 388, 889, 1525
HincII GTYRAC 1 cut(s) 1279
HindII GTYRAC 1 cut(s) 1279
HinfI GANTC 8 cut(s) 433, 474, 934, 975, 1060, 1351, 1515, 1554
HpaII CCGG 2 cut(s) 278, 779
HphI GGTGA 5 cut(s) 232, 517, 733, 1018, 1549
Hpy166II GTNNAC 4 cut(s) 159, 660, 1133, 1279
Hpy188I TCNGA 7 cut(s) 111, 612, 1389, 1396, 1483, 1514, 1553
Hpy188III TCNNGA 5 cut(s) 193, 694, 1192, 1519, 1581
Hpy8I GTNNAC 4 cut(s) 159, 660, 1133, 1279
Hpy99I CGWCG 3 cut(s) 433, 934, 1611
HpyAV CCTTC 2 cut(s) 1175, 1202
HpyCH4III ACNGT 2 cut(s) 530, 1218
HpyCH4IV ACGT 1 cut(s) 1029
HpyF10VI GCNNNNNNNGC 1 cut(s) 1122
HpyF3I CTNAG 2 cut(s) 1150, 1550
HpySE526I ACGT 1 cut(s) 1029
Hsp92II CATG 8 cut(s) 146, 152, 291, 388, 647, 653, 792, 889
HspAI GCGC 3 cut(s) 388, 889, 1525
KpnI GGTACC 2 cut(s) 327, 828
Ksp22I TGATCA 1 cut(s) 3
LweI GCATC 1 cut(s) 1449
MabI ACCWGGT 2 cut(s) 326, 827
MaeI CTAG 3 cut(s) 338, 839, 1637
MaeII ACGT 1 cut(s) 1029
MboII GAAGA 9 cut(s) 4, 229, 520, 730, 1021, 1051, 1322, 1442, 1505
MfeI CAATTG 2 cut(s) 1107, 1138
MflI RGATCY 5 cut(s) 66, 513, 567, 1014, 1500
MhlI GDGCHC 2 cut(s) 161, 662
MluCI AATT 8 cut(s) 1066, 1107, 1138, 1255, 1344, 1383, 1390, 1470
MlyI GAGTC 2 cut(s) 1524, 1548
MmeI TCCRAC 5 cut(s) 134, 479, 635, 980, 1612
MseI TTAA 5 cut(s) 74, 104, 575, 605, 1652
MslI CAYNNNNRTG 4 cut(s) 84, 147, 585, 648
MspA1I CMGCKG 2 cut(s) 353, 854
MspI CCGG 2 cut(s) 278, 779
MspR9I CCNGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
MunI CAATTG 2 cut(s) 1107, 1138
MvaI CCWGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
MwoI GCNNNNNNNGC 1 cut(s) 1122
NdeI CATATG 2 cut(s) 319, 820
NlaIII CATG 8 cut(s) 146, 152, 291, 388, 647, 653, 792, 889
NlaIV GGNNCC 5 cut(s) 68, 325, 569, 826, 1055
NsbI TGCGCA 2 cut(s) 389, 890
NspI RCATGY 2 cut(s) 152, 653
OliI CACNNNNGTG 2 cut(s) 84, 585
PasI CCCWGGG 2 cut(s) 206, 707
PciI ACATGT 2 cut(s) 148, 649
PfeI GAWTC 6 cut(s) 433, 474, 934, 975, 1060, 1351
PflFI GACNNNGTC 1 cut(s) 1587
PleI GAGTC 2 cut(s) 1523, 1548
PpsI GAGTC 2 cut(s) 1523, 1548
PscI ACATGT 2 cut(s) 148, 649
Psp6I CCWGG 7 cut(s) 25, 174, 205, 326, 675, 706, 827
PspFI CCCAGC 2 cut(s) 61, 562
PspGI CCWGG 7 cut(s) 25, 174, 205, 326, 675, 706, 827
PspN4I GGNNCC 5 cut(s) 68, 325, 569, 826, 1055
PspPI GGNCC 1 cut(s) 1143
PstNI CAGNNNCTG 2 cut(s) 356, 857
PsuI RGATCY 5 cut(s) 66, 513, 567, 1014, 1500
PsyI GACNNNGTC 1 cut(s) 1587
PvuII CAGCTG 2 cut(s) 353, 854
RsaI GTAC 5 cut(s) 252, 325, 753, 826, 1134
RsaNI GTAC 5 cut(s) 251, 324, 752, 825, 1133
RseI CAYNNNNRTG 4 cut(s) 84, 147, 585, 648
SaqAI TTAA 5 cut(s) 74, 104, 575, 605, 1652
Sau96I GGNCC 1 cut(s) 1143
SchI GAGTC 2 cut(s) 1524, 1548
ScrFI CCNGG 7 cut(s) 27, 176, 207, 328, 677, 708, 829
SduI GDGCHC 2 cut(s) 161, 662
SexAI ACCWGGT 2 cut(s) 326, 827
SfaNI GCATC 1 cut(s) 1449
SinI GGWCC 1 cut(s) 1143
SmiMI CAYNNNNRTG 4 cut(s) 84, 147, 585, 648
Sse9I AATT 8 cut(s) 1066, 1107, 1138, 1255, 1344, 1383, 1390, 1470
SspMI CTAG 3 cut(s) 338, 839, 1637
StyD4I CCNGG 7 cut(s) 25, 174, 205, 326, 675, 706, 827
TaaI ACNGT 2 cut(s) 530, 1218
TaiI ACGT 1 cut(s) 1032
TaqI TCGA 2 cut(s) 1284, 1591
TasI AATT 8 cut(s) 1066, 1107, 1138, 1255, 1344, 1383, 1390, 1470
TatI WGTACW 1 cut(s) 1132
TfiI GAWTC 6 cut(s) 433, 474, 934, 975, 1060, 1351
Tru1I TTAA 5 cut(s) 74, 104, 575, 605, 1652
Tru9I TTAA 5 cut(s) 74, 104, 575, 605, 1652
TscAI CASTG 2 cut(s) 528, 1029
TspDTI ATGAA 8 cut(s) 153, 159, 276, 654, 660, 777, 1343, 1463
TspGWI ACGGA 2 cut(s) 1566, 1595
TspRI CASTG 2 cut(s) 528, 1029
Tth111I GACNNNGTC 1 cut(s) 1587
VneI GTGCAC 2 cut(s) 157, 658
VpaK11BI GGWCC 1 cut(s) 1143
XapI RAATTY 5 cut(s) 1066, 1255, 1344, 1390, 1470
XceI RCATGY 2 cut(s) 152, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 1153
XspI CTAG 3 cut(s) 338, 839, 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.