Rw2G038070

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000689
Physical Location & Seq
Forward (+)
62896636 .. 62902397
5762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G038070.1

Sequence Viewer

Length: 909 bp
ATGGCCGAAACAGCAAAGCGCCTAGAAGGGTTTTGGATCCTGGATCCGCCCCTGCTTGCTAGTAATCTCACAGATGAAATTGGGCCAACACTCGCTAGAGGACATGACTTCATAAAAAAATCTCAGGTCAAGGACAACCCATCTGGTGATTTCAAAAGCATGCACCGCCACATTTCTAAAGGATCATGGACTTTCTCTGATCAAGATCATGGATGGCAAGTTTCTGATTGCACTGCAGAAGGTTTAAAGTGTTGCCTTCTGTTGTCGATGATGCCACTGGAGATAGTTGGAGAAAAATTGGAACCTGAGCGCTTGTACGATTCTGTCAATGTTCTACTTTCGCTACAGAGCAAGAATGGTGGTTTATCAGCCTGGGAACCAGCAGGGGCAGCAGAGTGGTTAGAACTGTTAAATCCGACAGAATTCTTCGCAGACATTGTGATTGAGCATGAATACGTTCAGTACACTTCATCTGCAATCCAGGCTTTAGTTTTGTTTAAAAAGCTAAACCCTGGCCACAGGAAGAAAGAGATCGAACATTCCATCACCAATGCTGTACACTACCTTGAAAATATACAAATGCCGGATGGTTCATGGTATGGAAATTGGGGAGTTTGCTTCATATATGGTACCTGGTTTGCGCTAGGAGGATTGGCAGCTGCTGGCAAGACTTTCAGAACTAGTGAGGCCATCGCAATGGGGTTAGTTTTCTACTTATGGCGCAGAGAGAGAACGGCGGTTGGGGAGAAAGCTATCTTTCATGTCCACAAAAGAAGGAAACCGATCCAATTTAGTACATACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAAGCAGAAAGAGACCCAACACCTCTTCATCGTGCAGCAAAGTTTATAATCAATTCTCAATTGGAAAATGGTGA

Protein Analysis

302

Amino Acids

33.99

Weight (kDa)

7.65

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_C PF13243 28 - 227 1.3e-22 Squalene-hopene cyclase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000689)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08040
malus_domestica MD09G1245800.v1.1 MD11G1098500.v1.1 MD16G1237300.v1.1
pyrus_communis pycom03g07090 pycom03g07100 pycom09g08560 pycom13g20460 pycom16g19890
rosa_chinensis RchiOBHm_Chr2g0118961 RchiOBHm_Chr2g0148671 RchiOBHm_Chr2g0150531 RchiOBHm_Chr2g0171301 RchiOBHm_Chr3g0455021 RchiOBHm_Chr6g0249451
rosa_laevigata RLG00000001225 RLG00000001972 RLG00000008936 RLG00000009011 RLG00000009032 RLG00000015372 RLG00000019992 RLG00000020680 RLG00000026543
rosa_multiflora Rmu_co8272705.1_g000001 Rmu_co8286409.1_g000001 Rmu_sc0000048.1_g000034 Rmu_sc0000824.1_g000002 Rmu_sc0001839.1_g000023 Rmu_sc0002070.1_g000050 Rmu_sc0002349.1_g000005 Rmu_sc0002453.1_g000019 Rmu_sc0006431.1_g000026 Rmu_sc0006758.1_g000001 Rmu_sc0006758.1_g000013 Rmu_sc0006997.1_g000015 Rmu_sc0008411.1_g000007 Rmu_sc0008411.1_g000019 Rmu_sc0008411.1_g000028 Rmu_sc0008678.1_g000001
rosa_roxburghii Rroxscaffold_3G00225850 Rroxscaffold_3G00225860 Rroxscaffold_3G00233460 Rroxscaffold_3G00234260 Rroxscaffold_3G00236880 Rroxscaffold_3G00236890 Rroxscaffold_4G00294300 Rroxscaffold_4G00297380 Rroxscaffold_4G00324650 Rroxscaffold_5G00352840 Rroxscaffold_5G00354640 Rroxscaffold_5G00361500 Rroxscaffold_7G00156410 Rroxscaffold_7G00156420 Rroxscaffold_7G00198110 Rroxscaffold_7G00198120
rosa_samantha Rh1CG014400 Rh1DG009900 Rh2AG465900 Rh2BG342900 Rh2CG452400 Rh2DG488000 Rh3DG303300 Rh4AG118000 Rh4DG159500 Rh5AG246100 Rh6AG232100 Rh6BG178700 Rh6DG174100 Rh7BG231200 Rh7DG380300
rosa_wichuraiana Rw2G027640 Rw2G038070 Rw6G005920 Rw7G034430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
Acc65I GGTACC 1 cut(s) 629
AccB1I GGYRCC 1 cut(s) 629
AciI CCGC 4 cut(s) 47, 166, 737, 831
AclWI GGATC 6 cut(s) 31, 38, 44, 51, 190, 778
AcoI YGGCCR 2 cut(s) 3, 514
AcsI RAATTY 1 cut(s) 422
AfaI GTAC 5 cut(s) 317, 464, 558, 631, 796
AfeI AGCGCT 1 cut(s) 311
AgsI TTSAA 2 cut(s) 154, 569
AhlI ACTAGT 1 cut(s) 680
AjnI CCWGG 5 cut(s) 39, 371, 480, 511, 632
AluBI AGCT 3 cut(s) 505, 659, 752
AluI AGCT 3 cut(s) 505, 659, 752
Alw26I GTCTC 2 cut(s) 825, 841
AlwI GGATC 6 cut(s) 31, 38, 44, 51, 190, 778
AlwNI CAGNNNCTG 1 cut(s) 662
Aor51HI AGCGCT 1 cut(s) 311
AoxI GGCC 4 cut(s) 3, 83, 514, 687
ApeKI GCWGC 4 cut(s) 389, 656, 659, 870
ApoI RAATTY 1 cut(s) 422
Asp700I GAANNNNTTC 1 cut(s) 456
Asp718I GGTACC 1 cut(s) 629
AspLEI GCGC 4 cut(s) 21, 312, 643, 723
AspS9I GGNCC 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 158, 538
BalI TGGCCA 1 cut(s) 516
BamHI GGATCC 2 cut(s) 36, 43
BanI GGYRCC 1 cut(s) 629
BbvI GCAGC 4 cut(s) 401, 646, 668, 882
BccI CCATC 6 cut(s) 148, 207, 551, 581, 698, 809
BceAI ACGGC 1 cut(s) 750
BciT130I CCWGG 5 cut(s) 41, 373, 482, 513, 634
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 2 cut(s) 825, 841
BcuI ACTAGT 1 cut(s) 680
BfaI CTAG 5 cut(s) 23, 60, 96, 644, 681
BfmI CTRYAG 2 cut(s) 234, 344
BfoI RGCGCY 2 cut(s) 22, 313
BisI GCNGC 4 cut(s) 390, 657, 660, 871
BlsI GCNGC 4 cut(s) 391, 658, 661, 872
Bme1390I CCNGG 5 cut(s) 41, 373, 482, 513, 634
BmgT120I GGNCC 1 cut(s) 83
BmiI GGNNCC 5 cut(s) 38, 45, 303, 378, 631
BmrFI CCNGG 5 cut(s) 41, 373, 482, 513, 634
BmsI GCATC 1 cut(s) 261
BpmI CTGGAG 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 306
BsaBI GATNNNNATC 1 cut(s) 204
BsaI GGTCTC 2 cut(s) 825, 841
BsaJI CCNNGG 2 cut(s) 372, 511
Bse1I ACTGG 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 702
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 5 cut(s) 41, 373, 482, 513, 634
BseDI CCNNGG 2 cut(s) 372, 511
BseGI GGATG 2 cut(s) 218, 592
BseJI GATNNNNATC 1 cut(s) 204
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 2 cut(s) 137, 297
BseNI ACTGG 1 cut(s) 282
BseXI GCAGC 4 cut(s) 401, 646, 668, 882
BsgI GTGCAG 1 cut(s) 889
BshFI GGCC 4 cut(s) 5, 85, 516, 689
BshNI GGYRCC 1 cut(s) 629
BsiSI CCGG 1 cut(s) 584
BslFI GGGAC 1 cut(s) 847
BsmAI GTCTC 2 cut(s) 825, 841
BsmFI GGGAC 1 cut(s) 847
BsnI GGCC 4 cut(s) 5, 85, 516, 689
Bso31I GGTCTC 2 cut(s) 825, 841
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
BspACI CCGC 4 cut(s) 47, 166, 737, 831
BspANI GGCC 4 cut(s) 5, 85, 516, 689
BspCNI CTCAG 2 cut(s) 136, 298
BspLI GGNNCC 5 cut(s) 38, 45, 303, 378, 631
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 6 cut(s) 31, 38, 44, 51, 190, 778
BspT107I GGYRCC 1 cut(s) 629
BspTNI GGTCTC 2 cut(s) 825, 841
BsrDI GCAATG 1 cut(s) 702
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 2 cut(s) 372, 511
BssMI GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
Bst2UI CCWGG 5 cut(s) 41, 373, 482, 513, 634
Bst4CI ACNGT 1 cut(s) 408
Bst6I CTCTTC 1 cut(s) 865
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 57, 161, 664
BstDEI CTNAG 2 cut(s) 123, 306
BstF5I GGATG 2 cut(s) 218, 592
BstH2I RGCGCY 2 cut(s) 22, 313
BstHHI GCGC 4 cut(s) 21, 312, 643, 723
BstKTI GATC 7 cut(s) 39, 46, 185, 202, 208, 534, 786
BstMAI GTCTC 2 cut(s) 825, 841
BstMBI GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
BstMWI GCNNNNNNNGC 5 cut(s) 11, 165, 389, 482, 837
BstNI CCWGG 5 cut(s) 41, 373, 482, 513, 634
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 5 cut(s) 39, 371, 480, 511, 632
BstSFI CTRYAG 2 cut(s) 234, 344
BstV1I GCAGC 4 cut(s) 401, 646, 668, 882
BstX2I RGATCY 2 cut(s) 36, 43
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 2 cut(s) 36, 43
BsuRI GGCC 4 cut(s) 5, 85, 516, 689
BtgZI GCGATG 1 cut(s) 676
BtsCI GGATG 2 cut(s) 218, 592
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 231, 275
Cac8I GCNNGC 3 cut(s) 57, 161, 664
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 4 cut(s) 21, 312, 643, 723
Cfr13I GGNCC 1 cut(s) 83
CsiI ACCWGGT 1 cut(s) 632
Csp6I GTAC 5 cut(s) 316, 463, 557, 630, 795
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 8 cut(s) 104, 160, 186, 209, 449, 594, 761, 828
CviQI GTAC 5 cut(s) 316, 463, 557, 630, 795
DdeI CTNAG 2 cut(s) 123, 306
DpnI GATC 7 cut(s) 38, 45, 184, 201, 207, 533, 785
DpnII GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
DraI TTTAAA 2 cut(s) 246, 499
EaeI YGGCCR 2 cut(s) 3, 514
Eam1104I CTCTTC 1 cut(s) 865
EarI CTCTTC 1 cut(s) 865
EciI GGCGGA 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 825, 841
Eco47III AGCGCT 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 5 cut(s) 39, 371, 480, 511, 632
FaeI CATG 8 cut(s) 107, 163, 189, 212, 452, 597, 764, 831
FaqI GGGAC 1 cut(s) 847
FatI CATG 8 cut(s) 103, 159, 185, 208, 448, 593, 760, 827
FauI CCCGC 1 cut(s) 824
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 4 cut(s) 390, 657, 660, 871
FokI GGATG 2 cut(s) 225, 599
Fsp4HI GCNGC 4 cut(s) 390, 657, 660, 871
FspBI CTAG 5 cut(s) 23, 60, 96, 644, 681
GlaI GCGC 4 cut(s) 20, 311, 642, 722
GluI GCNGC 4 cut(s) 390, 657, 660, 871
GsuI CTGGAG 1 cut(s) 299
HaeII RGCGCY 2 cut(s) 22, 313
HaeIII GGCC 4 cut(s) 5, 85, 516, 689
HapII CCGG 1 cut(s) 584
HhaI GCGC 4 cut(s) 21, 312, 643, 723
Hin1II CATG 8 cut(s) 107, 163, 189, 212, 452, 597, 764, 831
Hin6I GCGC 4 cut(s) 19, 310, 641, 721
HinP1I GCGC 4 cut(s) 19, 310, 641, 721
HinfI GANTC 1 cut(s) 320
HpaII CCGG 1 cut(s) 584
HphI GGTGA 2 cut(s) 158, 538
Hpy166II GTNNAC 3 cut(s) 465, 559, 766
Hpy188I TCNGA 4 cut(s) 199, 226, 417, 677
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 3 cut(s) 465, 559, 766
HpyAV CCTTC 4 cut(s) 20, 233, 266, 768
HpyCH4III ACNGT 1 cut(s) 408
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 5 cut(s) 163, 231, 236, 476, 870
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 165, 389, 482, 837
HpyF3I CTNAG 2 cut(s) 123, 306
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 8 cut(s) 107, 163, 189, 212, 452, 597, 764, 831
HspAI GCGC 4 cut(s) 19, 310, 641, 721
KpnI GGTACC 1 cut(s) 633
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
Lsp1109I GCAGC 4 cut(s) 401, 646, 668, 882
LweI GCATC 1 cut(s) 261
MabI ACCWGGT 1 cut(s) 632
MaeI CTAG 5 cut(s) 23, 60, 96, 644, 681
MaeII ACGT 1 cut(s) 456
MalI GATC 7 cut(s) 38, 45, 184, 201, 207, 533, 785
MboI GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
MboII GAAGA 3 cut(s) 418, 535, 852
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 2 cut(s) 36, 43
MlsI TGGCCA 1 cut(s) 516
MluCI AATT 7 cut(s) 78, 296, 422, 604, 788, 887, 894
MluNI TGGCCA 1 cut(s) 516
MmeI TCCRAC 2 cut(s) 268, 440
MnlI CCTC 4 cut(s) 92, 641, 679, 868
Mox20I TGGCCA 1 cut(s) 516
MroXI GAANNNNTTC 1 cut(s) 456
MscI TGGCCA 1 cut(s) 516
MseI TTAA 3 cut(s) 245, 410, 498
MslI CAYNNNNRTG 1 cut(s) 695
Msp20I TGGCCA 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 5 cut(s) 41, 373, 482, 513, 634
MunI CAATTG 1 cut(s) 894
MvaI CCWGG 5 cut(s) 41, 373, 482, 513, 634
MwoI GCNNNNNNNGC 5 cut(s) 11, 165, 389, 482, 837
NdeII GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
NlaIII CATG 8 cut(s) 107, 163, 189, 212, 452, 597, 764, 831
NlaIV GGNNCC 5 cut(s) 38, 45, 303, 378, 631
NspI RCATGY 1 cut(s) 163
PaeI GCATGC 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 320
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 4 cut(s) 391, 658, 661, 872
PsiI TTATAA 1 cut(s) 882
Psp6I CCWGG 5 cut(s) 39, 371, 480, 511, 632
PspGI CCWGG 5 cut(s) 39, 371, 480, 511, 632
PspN4I GGNNCC 5 cut(s) 38, 45, 303, 378, 631
PspPI GGNCC 1 cut(s) 83
PstI CTGCAG 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 2 cut(s) 36, 43
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 5 cut(s) 317, 464, 558, 631, 796
RsaNI GTAC 5 cut(s) 316, 463, 557, 630, 795
RseI CAYNNNNRTG 1 cut(s) 695
SaqAI TTAA 3 cut(s) 245, 410, 498
SatI GCNGC 4 cut(s) 390, 657, 660, 871
Sau3AI GATC 7 cut(s) 36, 43, 182, 199, 205, 531, 783
Sau96I GGNCC 1 cut(s) 83
ScrFI CCNGG 5 cut(s) 41, 373, 482, 513, 634
SexAI ACCWGGT 1 cut(s) 632
SfaNI GCATC 1 cut(s) 261
SfcI CTRYAG 2 cut(s) 234, 344
SmiMI CAYNNNNRTG 1 cut(s) 695
SpeI ACTAGT 1 cut(s) 680
SphI GCATGC 1 cut(s) 163
Sse9I AATT 7 cut(s) 78, 296, 422, 604, 788, 887, 894
SsiI CCGC 4 cut(s) 47, 166, 737, 831
SspMI CTAG 5 cut(s) 23, 60, 96, 644, 681
StyD4I CCNGG 5 cut(s) 39, 371, 480, 511, 632
TaaI ACNGT 1 cut(s) 408
TaiI ACGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 266, 534
TasI AATT 7 cut(s) 78, 296, 422, 604, 788, 887, 894
TatI WGTACW 3 cut(s) 462, 556, 794
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 3 cut(s) 245, 410, 498
Tru9I TTAA 3 cut(s) 245, 410, 498
TscAI CASTG 2 cut(s) 238, 282
TseI GCWGC 4 cut(s) 389, 656, 659, 870
TspDTI ATGAA 9 cut(s) 90, 100, 459, 465, 582, 610, 749, 816, 852
TspRI CASTG 2 cut(s) 238, 282
XapI RAATTY 1 cut(s) 422
XceI RCATGY 1 cut(s) 163
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 5 cut(s) 23, 60, 96, 644, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.