The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 35 |
| AccII | CGCG | 1 cut(s) | 480 |
| AciI | CCGC | 7 cut(s) | 4, 95, 200, 281, 408, 434, 467 |
| AclWI | GGATC | 2 cut(s) | 58, 94 |
| AcoI | YGGCCR | 1 cut(s) | 92 |
| AcsI | RAATTY | 2 cut(s) | 108, 329 |
| AcyI | GRCGYC | 1 cut(s) | 32 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 355, 378 |
| AflIII | ACRYGT | 1 cut(s) | 478 |
| AgsI | TTSAA | 2 cut(s) | 155, 236 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 479 |
| AjnI | CCWGG | 1 cut(s) | 186 |
| AluBI | AGCT | 2 cut(s) | 128, 366 |
| AluI | AGCT | 2 cut(s) | 128, 366 |
| Alw21I | GWGCWC | 1 cut(s) | 223 |
| Alw26I | GTCTC | 2 cut(s) | 23, 329 |
| AlwI | GGATC | 2 cut(s) | 58, 94 |
| AoxI | GGCC | 5 cut(s) | 36, 82, 92, 201, 380 |
| ApeKI | GCWGC | 1 cut(s) | 461 |
| ApoI | RAATTY | 2 cut(s) | 108, 329 |
| AseI | ATTAAT | 1 cut(s) | 132 |
| AspS9I | GGNCC | 5 cut(s) | 6, 37, 82, 202, 410 |
| AsuC2I | CCSGG | 1 cut(s) | 91 |
| AvaII | GGWCC | 2 cut(s) | 6, 410 |
| BanII | GRGCYC | 1 cut(s) | 174 |
| BbsI | GAAGAC | 1 cut(s) | 392 |
| Bbv12I | GWGCWC | 1 cut(s) | 223 |
| BbvI | GCAGC | 1 cut(s) | 448 |
| BccI | CCATC | 2 cut(s) | 111, 455 |
| BceAI | ACGGC | 1 cut(s) | 413 |
| BciT130I | CCWGG | 1 cut(s) | 188 |
| BcnI | CCSGG | 1 cut(s) | 91 |
| BcoDI | GTCTC | 2 cut(s) | 23, 329 |
| BfaI | CTAG | 1 cut(s) | 278 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 434 |
| BglII | AGATCT | 1 cut(s) | 157 |
| BisI | GCNGC | 4 cut(s) | 95, 201, 435, 462 |
| BlpI | GCTNAGC | 1 cut(s) | 362 |
| BlsI | GCNGC | 4 cut(s) | 96, 202, 436, 463 |
| Bme1390I | CCNGG | 2 cut(s) | 91, 188 |
| Bme18I | GGWCC | 2 cut(s) | 6, 410 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 479 |
| BmgT120I | GGNCC | 5 cut(s) | 6, 37, 82, 202, 410 |
| BmiI | GGNNCC | 1 cut(s) | 173 |
| BmrFI | CCNGG | 2 cut(s) | 91, 188 |
| BmrI | ACTGGG | 1 cut(s) | 306 |
| BmsI | GCATC | 3 cut(s) | 48, 111, 309 |
| BmuI | ACTGGG | 1 cut(s) | 306 |
| BpiI | GAAGAC | 1 cut(s) | 392 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 362 |
| BpuMI | CCSGG | 1 cut(s) | 91 |
| BsaHI | GRCGYC | 1 cut(s) | 32 |
| BsaI | GGTCTC | 1 cut(s) | 329 |
| BsaJI | CCNNGG | 4 cut(s) | 89, 178, 354, 383 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 355, 378 |
| Bse1I | ACTGG | 1 cut(s) | 312 |
| BseBI | CCWGG | 1 cut(s) | 188 |
| BseDI | CCNNGG | 4 cut(s) | 89, 178, 354, 383 |
| BseGI | GGATG | 1 cut(s) | 460 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 355, 378 |
| BseMII | CTCAG | 1 cut(s) | 353 |
| BseNI | ACTGG | 1 cut(s) | 312 |
| BseX3I | CGGCCG | 1 cut(s) | 92 |
| BseXI | GCAGC | 1 cut(s) | 448 |
| BseYI | CCCAGC | 1 cut(s) | 371 |
| BsgI | GTGCAG | 1 cut(s) | 447 |
| Bsh1236I | CGCG | 1 cut(s) | 480 |
| Bsh1285I | CGRYCG | 1 cut(s) | 95 |
| BshFI | GGCC | 5 cut(s) | 38, 84, 94, 203, 382 |
| BsiEI | CGRYCG | 1 cut(s) | 95 |
| BsiHKAI | GWGCWC | 1 cut(s) | 223 |
| BsiSI | CCGG | 1 cut(s) | 91 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 355, 378 |
| BsmAI | GTCTC | 2 cut(s) | 23, 329 |
| BsmBI | CGTCTC | 1 cut(s) | 23 |
| BsnI | GGCC | 5 cut(s) | 38, 84, 94, 203, 382 |
| Bso31I | GGTCTC | 1 cut(s) | 329 |
| Bsp1286I | GDGCHC | 2 cut(s) | 174, 223 |
| Bsp143I | GATC | 3 cut(s) | 50, 99, 157 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 362 |
| Bsp19I | CCATGG | 1 cut(s) | 383 |
| BspACI | CCGC | 7 cut(s) | 4, 95, 200, 281, 408, 434, 467 |
| BspANI | GGCC | 5 cut(s) | 38, 84, 94, 203, 382 |
| BspCNI | CTCAG | 1 cut(s) | 354 |
| BspFNI | CGCG | 1 cut(s) | 480 |
| BspHI | TCATGA | 1 cut(s) | 25 |
| BspLI | GGNNCC | 1 cut(s) | 173 |
| BspPI | GGATC | 2 cut(s) | 58, 94 |
| BspTNI | GGTCTC | 1 cut(s) | 329 |
| BsrI | ACTGG | 1 cut(s) | 312 |
| BssECI | CCNNGG | 4 cut(s) | 89, 178, 354, 383 |
| BssMI | GATC | 3 cut(s) | 50, 99, 157 |
| BssNI | GRCGYC | 1 cut(s) | 32 |
| BssT1I | CCWWGG | 2 cut(s) | 354, 383 |
| Bst2UI | CCWGG | 1 cut(s) | 188 |
| Bst4CI | ACNGT | 2 cut(s) | 179, 350 |
| BstACI | GRCGYC | 1 cut(s) | 32 |
| BstC8I | GCNNGC | 2 cut(s) | 86, 126 |
| BstDEI | CTNAG | 1 cut(s) | 362 |
| BstDSI | CCRYGG | 2 cut(s) | 178, 383 |
| BstF5I | GGATG | 1 cut(s) | 460 |
| BstFNI | CGCG | 1 cut(s) | 480 |
| BstKTI | GATC | 3 cut(s) | 53, 102, 160 |
| BstMAI | GTCTC | 2 cut(s) | 23, 329 |
| BstMBI | GATC | 3 cut(s) | 50, 99, 157 |
| BstMCI | CGRYCG | 1 cut(s) | 95 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 44, 94, 146, 169, 372, 434, 443 |
| BstNI | CCWGG | 1 cut(s) | 188 |
| BstSCI | CCNGG | 2 cut(s) | 89, 186 |
| BstUI | CGCG | 1 cut(s) | 480 |
| BstV1I | GCAGC | 1 cut(s) | 448 |
| BstV2I | GAAGAC | 1 cut(s) | 392 |
| BstX2I | RGATCY | 2 cut(s) | 99, 157 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 212 |
| BstYI | RGATCY | 2 cut(s) | 99, 157 |
| BstZI | CGGCCG | 1 cut(s) | 92 |
| BsuRI | GGCC | 5 cut(s) | 38, 84, 94, 203, 382 |
| BtgI | CCRYGG | 2 cut(s) | 178, 383 |
| BtsCI | GGATG | 1 cut(s) | 460 |
| BtsI | GCAGTG | 1 cut(s) | 223 |
| BtsIMutI | CAGTG | 2 cut(s) | 60, 223 |
| Cac8I | GCNNGC | 2 cut(s) | 86, 126 |
| CciI | TCATGA | 1 cut(s) | 25 |
| Cfr13I | GGNCC | 5 cut(s) | 6, 37, 82, 202, 410 |
| CseI | GACGC | 2 cut(s) | 467, 469 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 164, 199 |
| CviAII | CATG | 3 cut(s) | 13, 26, 384 |
| DdeI | CTNAG | 1 cut(s) | 362 |
| DpnI | GATC | 3 cut(s) | 52, 101, 159 |
| DpnII | GATC | 3 cut(s) | 50, 99, 157 |
| DriI | GACNNNNNGTC | 1 cut(s) | 479 |
| EaeI | YGGCCR | 1 cut(s) | 92 |
| EagI | CGGCCG | 1 cut(s) | 92 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 479 |
| EclXI | CGGCCG | 1 cut(s) | 92 |
| Eco130I | CCWWGG | 2 cut(s) | 354, 383 |
| Eco24I | GRGCYC | 1 cut(s) | 174 |
| Eco31I | GGTCTC | 1 cut(s) | 329 |
| Eco47I | GGWCC | 2 cut(s) | 6, 410 |
| Eco52I | CGGCCG | 1 cut(s) | 92 |
| EcoRII | CCWGG | 1 cut(s) | 186 |
| EcoT14I | CCWWGG | 2 cut(s) | 354, 383 |
| EcoT22I | ATGCAT | 1 cut(s) | 265 |
| EcoT38I | GRGCYC | 1 cut(s) | 174 |
| ErhI | CCWWGG | 2 cut(s) | 354, 383 |
| Esp3I | CGTCTC | 1 cut(s) | 23 |
| FaeI | CATG | 3 cut(s) | 16, 29, 387 |
| FaiI | YATR | 5 cut(s) | 14, 27, 261, 385, 405 |
| FatI | CATG | 3 cut(s) | 12, 25, 383 |
| FauI | CCCGC | 1 cut(s) | 274 |
| Fnu4HI | GCNGC | 4 cut(s) | 95, 201, 435, 462 |
| FokI | GGATG | 1 cut(s) | 467 |
| FriOI | GRGCYC | 1 cut(s) | 174 |
| Fsp4HI | GCNGC | 4 cut(s) | 95, 201, 435, 462 |
| FspBI | CTAG | 1 cut(s) | 278 |
| GluI | GCNGC | 4 cut(s) | 95, 201, 435, 462 |
| GsaI | CCCAGC | 1 cut(s) | 375 |
| HaeIII | GGCC | 5 cut(s) | 38, 84, 94, 203, 382 |
| HapII | CCGG | 1 cut(s) | 91 |
| HgaI | GACGC | 2 cut(s) | 467, 469 |
| Hin1I | GRCGYC | 1 cut(s) | 32 |
| Hin1II | CATG | 3 cut(s) | 16, 29, 387 |
| HinfI | GANTC | 3 cut(s) | 22, 75, 397 |
| HpaII | CCGG | 1 cut(s) | 91 |
| Hpy188I | TCNGA | 2 cut(s) | 120, 242 |
| Hpy188III | TCNNGA | 1 cut(s) | 26 |
| Hpy99I | CGWCG | 2 cut(s) | 37, 485 |
| HpyCH4III | ACNGT | 2 cut(s) | 179, 350 |
| HpyCH4IV | ACGT | 1 cut(s) | 32 |
| HpyCH4V | TGCA | 4 cut(s) | 47, 124, 263, 464 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 44, 94, 146, 169, 372, 434, 443 |
| HpyF3I | CTNAG | 1 cut(s) | 362 |
| HpySE526I | ACGT | 1 cut(s) | 32 |
| Hsp92I | GRCGYC | 1 cut(s) | 32 |
| Hsp92II | CATG | 3 cut(s) | 16, 29, 387 |
| Kzo9I | GATC | 3 cut(s) | 50, 99, 157 |
| LmnI | GCTCC | 1 cut(s) | 177 |
| LpnPI | CCDG | 9 cut(s) | 22, 33, 53, 98, 104, 173, 200, 325, 385 |
| Lsp1109I | GCAGC | 1 cut(s) | 448 |
| LweI | GCATC | 3 cut(s) | 48, 111, 309 |
| MaeI | CTAG | 1 cut(s) | 278 |
| MaeII | ACGT | 1 cut(s) | 32 |
| MaeIII | GTNAC | 1 cut(s) | 344 |
| MalI | GATC | 3 cut(s) | 52, 101, 159 |
| MboI | GATC | 3 cut(s) | 50, 99, 157 |
| MboII | GAAGA | 2 cut(s) | 167, 392 |
| MflI | RGATCY | 2 cut(s) | 99, 157 |
| MhlI | GDGCHC | 2 cut(s) | 174, 223 |
| MluCI | AATT | 2 cut(s) | 108, 329 |
| MluI | ACGCGT | 1 cut(s) | 478 |
| MlyI | GAGTC | 1 cut(s) | 406 |
| MnlI | CCTC | 2 cut(s) | 372, 423 |
| Mph1103I | ATGCAT | 1 cut(s) | 265 |
| MseI | TTAA | 2 cut(s) | 132, 144 |
| MspI | CCGG | 1 cut(s) | 91 |
| MspR9I | CCNGG | 2 cut(s) | 91, 188 |
| MvaI | CCWGG | 1 cut(s) | 188 |
| MvnI | CGCG | 1 cut(s) | 480 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 44, 94, 146, 169, 372, 434, 443 |
| NciI | CCSGG | 1 cut(s) | 91 |
| NcoI | CCATGG | 1 cut(s) | 383 |
| NdeII | GATC | 3 cut(s) | 50, 99, 157 |
| NlaIII | CATG | 3 cut(s) | 16, 29, 387 |
| NlaIV | GGNNCC | 1 cut(s) | 173 |
| NsiI | ATGCAT | 1 cut(s) | 265 |
| PagI | TCATGA | 1 cut(s) | 25 |
| PfeI | GAWTC | 2 cut(s) | 22, 75 |
| PkrI | GCNGC | 4 cut(s) | 96, 202, 436, 463 |
| PleI | GAGTC | 1 cut(s) | 405 |
| PpsI | GAGTC | 1 cut(s) | 405 |
| PshBI | ATTAAT | 1 cut(s) | 132 |
| Psp6I | CCWGG | 1 cut(s) | 186 |
| PspFI | CCCAGC | 1 cut(s) | 371 |
| PspGI | CCWGG | 1 cut(s) | 186 |
| PspN4I | GGNNCC | 1 cut(s) | 173 |
| PspPI | GGNCC | 5 cut(s) | 6, 37, 82, 202, 410 |
| PsuI | RGATCY | 2 cut(s) | 99, 157 |
| SaqAI | TTAA | 2 cut(s) | 132, 144 |
| SatI | GCNGC | 4 cut(s) | 95, 201, 435, 462 |
| Sau3AI | GATC | 3 cut(s) | 50, 99, 157 |
| Sau96I | GGNCC | 5 cut(s) | 6, 37, 82, 202, 410 |
| SchI | GAGTC | 1 cut(s) | 406 |
| ScrFI | CCNGG | 2 cut(s) | 91, 188 |
| SduI | GDGCHC | 2 cut(s) | 174, 223 |
| SetI | ASST | 5 cut(s) | 35, 130, 275, 368, 415 |
| SfaNI | GCATC | 3 cut(s) | 48, 111, 309 |
| SinI | GGWCC | 2 cut(s) | 6, 410 |
| Sse9I | AATT | 2 cut(s) | 108, 329 |
| SsiI | CCGC | 7 cut(s) | 4, 95, 200, 281, 408, 434, 467 |
| SspMI | CTAG | 1 cut(s) | 278 |
| StyD4I | CCNGG | 2 cut(s) | 89, 186 |
| StyI | CCWWGG | 2 cut(s) | 354, 383 |
| TaaI | ACNGT | 2 cut(s) | 179, 350 |
| TaiI | ACGT | 1 cut(s) | 35 |
| TaqI | TCGA | 3 cut(s) | 415, 472, 483 |
| TasI | AATT | 2 cut(s) | 108, 329 |
| TauI | GCSGC | 3 cut(s) | 97, 203, 437 |
| TfiI | GAWTC | 2 cut(s) | 22, 75 |
| Tru1I | TTAA | 2 cut(s) | 132, 144 |
| Tru9I | TTAA | 2 cut(s) | 132, 144 |
| TscAI | CASTG | 2 cut(s) | 60, 223 |
| TseI | GCWGC | 1 cut(s) | 461 |
| TspDTI | ATGAA | 2 cut(s) | 14, 392 |
| TspRI | CASTG | 2 cut(s) | 60, 223 |
| VpaK11BI | GGWCC | 2 cut(s) | 6, 410 |
| VspI | ATTAAT | 1 cut(s) | 132 |
| XapI | RAATTY | 2 cut(s) | 108, 329 |
| XspI | CTAG | 1 cut(s) | 278 |
| ZraI | GACGTC | 1 cut(s) | 33 |
| Zsp2I | ATGCAT | 1 cut(s) | 265 |