Rmu_sc0000173.1_g000039

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000173.1
OG0000565
Physical Location & Seq
Forward (+)
165567 .. 167886
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000173.1_g000039.1.cds

Sequence Viewer

Length: 657 bp
atgacgtcgaccgagacggaggacgccgtacgacgtcgcatcgccatcaccgatcactgcaagagattcgcactccaaatcctccttattcatgttcgattctgcatctgcaaccgccattgccgaaccggctcacaagcaaattcgaaagtccagagagcttcatctgcacctgaagagttgagatcttatcaaggaggatcgattcagctcttgcaggatccgaagcaccatacaaattcaaagattgggctcaggaagggatccagttcatttaatgaagctcgcgcagctggtttcaccgaggagaatgggactttaggtgacatatgggaaacaatctcagagagtgatcttgtgttgctactaatttctgatcccgaacagggcatgttggaaatatacagctctttgtctgaagctgaaaagaaggagtttgagactgcatatagtgcctcttattatccttgcatggacatcctctatgaatgctatgaggatgtagcaagcggcagtgagatccgtagcattgttttggctgggcggcgattttatgagaaagatggtcttcctgccttccctatgggaaagattgatcagacgcgcatgtggaaagttggtgagcgtgtccgatcgacagaatgcagtggattgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

218

Amino Acids

24.52

Weight (kDa)

5.8

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000565)

Species Orthologous Gene IDs
pyrus_communis pycom10g08490
rosa_chinensis RchiOBHm_Chr1g0329691 RchiOBHm_Chr1g0332621 RchiOBHm_Chr2g0113681 RchiOBHm_Chr3g0494941 RchiOBHm_Chr4g0405101 RchiOBHm_Chr4g0432031 RchiOBHm_Chr6g0256671 RchiOBHm_Chr6g0271971
rosa_laevigata RLG00000000812 RLG00000014208 RLG00000019091 RLG00000023770 RLG00000024432 RLG00000030162
rosa_multiflora Rmu_sc0000173.1_g000039 Rmu_sc0000213.1_g000001 Rmu_sc0000580.1_g000059 Rmu_sc0001084.1_g000007 Rmu_sc0004755.1_g000037 Rmu_sc0005523.1_g000016 Rmu_sc0006670.1_g000025 Rmu_sc0021593.1_g000003
rosa_roxburghii Rroxscaffold_176G00431130 Rroxscaffold_1G00016960 Rroxscaffold_1G00025070 Rroxscaffold_1G00041460 Rroxscaffold_4G00294040 Rroxscaffold_6G00409240 Rroxscaffold_6G00423240 Rroxscaffold_6G00425100 Rroxscaffold_7G00185440 Rroxscaffold_7G00204100
rosa_rugosa Rorug03G0304000 Rorug03G0304000 Rorug06G0059000 Rorug06G0112900
rosa_samantha Rh1AG075200 Rh1AG157000 Rh1AG157100 Rh1AG161600 Rh1BG065800 Rh1BG094200 Rh2AG675300 Rh2BG242500 Rh2BG287600 Rh2BG319100 Rh2BG494800 Rh2BG686600 Rh2CG248000 Rh2CG288100 Rh2CG288200 Rh2CG649800 Rh2DG603100 Rh2DG700700 Rh3AG253500 Rh3BG236900 Rh3CG110600 Rh3CG318000 Rh3DG231400 Rh4CG141900 Rh5BG429900 Rh5CG251900 Rh5CG266000 Rh5CG350700 Rh5DG443300 Rh6AG032600 Rh6AG032700 Rh6BG408900 Rh6CG008400 Rh6DG009300 Rh7AG279000 Rh7BG261100 Rh7CG160900 Rh7CG298200 Rh7CG465100 Rh7CG502800 Rh7DG293900 Rh7DG471100 Rh7DG471300
rosa_wichuraiana Rw0G009600 Rw1G010610 Rw3G018660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 8, 37
AccI GTMKAC 1 cut(s) 8
AccII CGCG 2 cut(s) 288, 604
AciI CCGC 3 cut(s) 115, 510, 544
AclWI GGATC 7 cut(s) 208, 215, 228, 258, 271, 371, 514
AcsI RAATTY 2 cut(s) 142, 238
AcuI CTGAAG 2 cut(s) 195, 438
AcyI GRCGYC 3 cut(s) 5, 24, 34
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 386
AgsI TTSAA 1 cut(s) 243
AluBI AGCT 6 cut(s) 161, 211, 284, 293, 408, 422
AluI AGCT 6 cut(s) 161, 211, 284, 293, 408, 422
Alw26I GTCTC 2 cut(s) 8, 434
AlwI GGATC 7 cut(s) 208, 215, 228, 258, 271, 371, 514
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 2 cut(s) 142, 238
AspLEI GCGC 2 cut(s) 290, 606
AsuHPI GGTGA 4 cut(s) 40, 292, 335, 632
AsuII TTCGAA 1 cut(s) 146
BamHI GGATCC 2 cut(s) 220, 263
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 560
BbvI GCAGC 1 cut(s) 302
BccI CCATC 2 cut(s) 53, 557
BceAI ACGGC 1 cut(s) 11
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 2 cut(s) 8, 434
BglI GCCNNNNNGGC 1 cut(s) 129
BglII AGATCT 1 cut(s) 185
BisI GCNGC 3 cut(s) 291, 511, 545
BlsI GCNGC 3 cut(s) 292, 512, 546
BmiI GGNNCC 2 cut(s) 222, 265
BmsI GCATC 2 cut(s) 48, 114
BpiI GAAGAC 1 cut(s) 560
Bpu10I CCTNAGC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 203
BsaHI GRCGYC 3 cut(s) 5, 24, 34
BsaJI CCNNGG 1 cut(s) 303
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 267
Bse3DI GCAATG 1 cut(s) 118
BseCI ATCGAT 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 2 cut(s) 477, 505
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 2 cut(s) 268, 357
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 302
BseYI CCCAGC 1 cut(s) 539
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 2 cut(s) 288, 604
Bsh1285I CGRYCG 2 cut(s) 12, 635
BshVI ATCGAT 1 cut(s) 203
BsiEI CGRYCG 2 cut(s) 12, 635
BsiSI CCGG 1 cut(s) 129
BsiWI CGTACG 1 cut(s) 28
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 2 cut(s) 8, 434
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 2 cut(s) 494, 647
Bsp119I TTCGAA 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 255
BspACI CCGC 3 cut(s) 115, 510, 544
BspCNI CTCAG 2 cut(s) 267, 356
BspDI ATCGAT 1 cut(s) 203
BspFNI CGCG 2 cut(s) 288, 604
BspLI GGNNCC 2 cut(s) 222, 265
BspPI GGATC 7 cut(s) 208, 215, 228, 258, 271, 371, 514
BspT104I TTCGAA 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 303
BssNI GRCGYC 3 cut(s) 5, 24, 34
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 3 cut(s) 5, 24, 34
BstAPI GCANNNNNTGC 1 cut(s) 452
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 286, 508
BstDEI CTNAG 2 cut(s) 254, 343
BstF5I GGATG 2 cut(s) 477, 505
BstFNI CGCG 2 cut(s) 288, 604
BstHHI GCGC 2 cut(s) 290, 606
BstMAI GTCTC 2 cut(s) 8, 434
BstMCI CGRYCG 2 cut(s) 12, 635
BstMWI GCNNNNNNNGC 4 cut(s) 129, 167, 290, 452
BstNSI RCATGY 2 cut(s) 394, 610
BstUI CGCG 2 cut(s) 288, 604
BstV1I GCAGC 1 cut(s) 302
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 4 cut(s) 185, 220, 263, 519
BstYI RGATCY 4 cut(s) 185, 220, 263, 519
Bsu15I ATCGAT 1 cut(s) 203
BsuTUI ATCGAT 1 cut(s) 203
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 2 cut(s) 477, 505
BtsI GCAGTG 3 cut(s) 55, 520, 652
BtsIMutI CAGTG 3 cut(s) 55, 520, 652
Cac8I GCNNGC 2 cut(s) 286, 508
CfoI GCGC 2 cut(s) 290, 606
Cfr10I RCCGGY 1 cut(s) 128
ClaI ATCGAT 1 cut(s) 203
CseI GACGC 2 cut(s) 32, 610
Csp6I GTAC 1 cut(s) 29
CviAII CATG 4 cut(s) 92, 391, 472, 607
CviJI RGCY 9 cut(s) 132, 161, 211, 253, 284, 293, 408, 422, 539
CviKI_1 RGCY 9 cut(s) 132, 161, 211, 253, 284, 293, 408, 422, 539
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 2 cut(s) 254, 343
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 255
Eco57I CTGAAG 2 cut(s) 195, 438
EcoT38I GRGCYC 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 4 cut(s) 95, 394, 475, 610
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FaqI GGGAC 1 cut(s) 328
FatI CATG 4 cut(s) 91, 390, 471, 606
FauNDI CATATG 1 cut(s) 329
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 3 cut(s) 291, 511, 545
FokI GGATG 2 cut(s) 464, 512
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 3 cut(s) 291, 511, 545
GlaI GCGC 2 cut(s) 289, 605
GluI GCNGC 3 cut(s) 291, 511, 545
GsaI CCCAGC 1 cut(s) 543
HapII CCGG 1 cut(s) 129
HgaI GACGC 2 cut(s) 32, 610
HhaI GCGC 2 cut(s) 290, 606
Hin1I GRCGYC 3 cut(s) 5, 24, 34
Hin1II CATG 4 cut(s) 95, 394, 475, 610
Hin6I GCGC 2 cut(s) 288, 604
HinP1I GCGC 2 cut(s) 288, 604
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 3 cut(s) 66, 99, 205
HpaII CCGG 1 cut(s) 129
HphI GGTGA 4 cut(s) 40, 292, 335, 632
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 6 cut(s) 225, 346, 376, 418, 600, 632
Hpy188III TCNNGA 3 cut(s) 154, 256, 380
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 3 cut(s) 10, 36, 39
HpyAV CCTTC 3 cut(s) 253, 424, 586
HpyCH4IV ACGT 2 cut(s) 5, 34
HpyCH4V TGCA 8 cut(s) 60, 105, 111, 170, 217, 446, 471, 645
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 167, 290, 452
HpyF3I CTNAG 2 cut(s) 254, 343
HpySE526I ACGT 2 cut(s) 5, 34
Hsp92I GRCGYC 3 cut(s) 5, 24, 34
Hsp92II CATG 4 cut(s) 95, 394, 475, 610
HspAI GCGC 2 cut(s) 288, 604
Ksp22I TGATCA 1 cut(s) 595
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 2 cut(s) 48, 114
MaeII ACGT 2 cut(s) 5, 34
MaeIII GTNAC 1 cut(s) 323
MboII GAAGA 2 cut(s) 188, 560
MflI RGATCY 4 cut(s) 185, 220, 263, 519
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 3 cut(s) 142, 238, 369
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 7 cut(s) 13, 92, 191, 298, 466, 490, 491
MseI TTAA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 129
Mva1269I GAATGC 2 cut(s) 494, 647
MvnI CGCG 2 cut(s) 288, 604
MwoI GCNNNNNNNGC 4 cut(s) 129, 167, 290, 452
NdeI CATATG 1 cut(s) 329
NlaIII CATG 4 cut(s) 95, 394, 475, 610
NlaIV GGNNCC 2 cut(s) 222, 265
NmuCI GTSAC 1 cut(s) 323
NspI RCATGY 2 cut(s) 394, 610
NspV TTCGAA 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 2 cut(s) 494, 647
PfeI GAWTC 3 cut(s) 66, 99, 205
Pfl23II CGTACG 1 cut(s) 28
PkrI GCNGC 3 cut(s) 292, 512, 546
Ple19I CGATCG 1 cut(s) 635
PspFI CCCAGC 1 cut(s) 539
PspLI CGTACG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 222, 265
PsuI RGATCY 4 cut(s) 185, 220, 263, 519
PvuI CGATCG 1 cut(s) 635
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 3 cut(s) 291, 511, 545
SduI GDGCHC 1 cut(s) 255
SfaNI GCATC 2 cut(s) 48, 114
SfuI TTCGAA 1 cut(s) 146
Sse9I AATT 3 cut(s) 142, 238, 369
SsiI CCGC 3 cut(s) 115, 510, 544
TaiI ACGT 2 cut(s) 8, 37
TaqI TCGA 5 cut(s) 8, 97, 146, 203, 635
TaqII GACCGA 1 cut(s) 26
TasI AATT 3 cut(s) 142, 238, 369
TauI GCSGC 2 cut(s) 513, 547
TfiI GAWTC 3 cut(s) 66, 99, 205
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 3 cut(s) 62, 520, 652
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 5 cut(s) 80, 153, 261, 294, 501
TspGWI ACGGA 2 cut(s) 32, 512
TspRI CASTG 3 cut(s) 62, 520, 652
XapI RAATTY 2 cut(s) 142, 238
XceI RCATGY 2 cut(s) 394, 610
XmiI GTMKAC 1 cut(s) 8
ZraI GACGTC 2 cut(s) 6, 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.