Rh1BG065800

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000565
Physical Location & Seq
Forward (+)
10015880 .. 10016395
516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG065800.1

Sequence Viewer

Length: 249 bp
ATGGGCGGACCTGCTCAAGCTCAGAACTTAAGAGATTCTCTAGCTGAAGCAAAGTTGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTTGCGCAGCTGGTTTCACCGAGGAGAATGGGACTTTAGGTGACATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCCGCACAGGTATGTTGGTTCTGCATTGGAAAGTCGTACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

82

Amino Acids

8.83

Weight (kDa)

4.69

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 3 - 71 3.7e-06 Acetohydroxy acid isomeroreductase, NADPH-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000565)

Species Orthologous Gene IDs
pyrus_communis pycom10g08490
rosa_chinensis RchiOBHm_Chr1g0329691 RchiOBHm_Chr1g0332621 RchiOBHm_Chr2g0113681 RchiOBHm_Chr3g0494941 RchiOBHm_Chr4g0405101 RchiOBHm_Chr4g0432031 RchiOBHm_Chr6g0256671 RchiOBHm_Chr6g0271971
rosa_laevigata RLG00000000812 RLG00000014208 RLG00000019091 RLG00000023770 RLG00000024432 RLG00000030162
rosa_multiflora Rmu_sc0000173.1_g000039 Rmu_sc0000213.1_g000001 Rmu_sc0000580.1_g000059 Rmu_sc0001084.1_g000007 Rmu_sc0004755.1_g000037 Rmu_sc0005523.1_g000016 Rmu_sc0006670.1_g000025 Rmu_sc0021593.1_g000003
rosa_roxburghii Rroxscaffold_176G00431130 Rroxscaffold_1G00016960 Rroxscaffold_1G00025070 Rroxscaffold_1G00041460 Rroxscaffold_4G00294040 Rroxscaffold_6G00409240 Rroxscaffold_6G00423240 Rroxscaffold_6G00425100 Rroxscaffold_7G00185440 Rroxscaffold_7G00204100
rosa_rugosa Rorug03G0304000 Rorug03G0304000 Rorug06G0059000 Rorug06G0112900
rosa_samantha Rh1AG075200 Rh1AG157000 Rh1AG157100 Rh1AG161600 Rh1BG065800 Rh1BG094200 Rh2AG675300 Rh2BG242500 Rh2BG287600 Rh2BG319100 Rh2BG494800 Rh2BG686600 Rh2CG248000 Rh2CG288100 Rh2CG288200 Rh2CG649800 Rh2DG603100 Rh2DG700700 Rh3AG253500 Rh3BG236900 Rh3CG110600 Rh3CG318000 Rh3DG231400 Rh4CG141900 Rh5BG429900 Rh5CG251900 Rh5CG266000 Rh5CG350700 Rh5DG443300 Rh6AG032600 Rh6AG032700 Rh6BG408900 Rh6CG008400 Rh6DG009300 Rh7AG279000 Rh7BG261100 Rh7CG160900 Rh7CG298200 Rh7CG465100 Rh7CG502800 Rh7DG293900 Rh7DG471100 Rh7DG471300
rosa_wichuraiana Rw0G009600 Rw1G010610 Rw3G018660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 115
Acc36I ACCTGC 1 cut(s) 19
AciI CCGC 2 cut(s) 6, 207
AclWI GGATC 3 cut(s) 84, 97, 197
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 1 cut(s) 242
AflII CTTAAG 1 cut(s) 28
AgsI TTSAA 1 cut(s) 58
AluBI AGCT 4 cut(s) 20, 44, 110, 119
AluI AGCT 4 cut(s) 20, 44, 110, 119
AlwI GGATC 3 cut(s) 84, 97, 197
ApeKI GCWGC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 2 cut(s) 118, 161
AvaII GGWCC 1 cut(s) 8
BamHI GGATCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 128
BfaI CTAG 1 cut(s) 41
BfrI CTTAAG 1 cut(s) 28
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 1 cut(s) 91
Bpu10I CCTNAGC 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 129
BseMII CTCAG 3 cut(s) 35, 94, 183
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 128
BslFI GGGAC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 3 cut(s) 89, 178, 202
BspACI CCGC 2 cut(s) 6, 207
BspCNI CTCAG 3 cut(s) 34, 93, 182
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 19
BspPI GGATC 3 cut(s) 84, 97, 197
BspTI CTTAAG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 3 cut(s) 89, 178, 202
BstAFI CTTAAG 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 3 cut(s) 21, 80, 169
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 92, 181, 205
BstMBI GATC 3 cut(s) 89, 178, 202
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 112
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 241
CviJI RGCY 5 cut(s) 20, 44, 79, 110, 119
CviKI_1 RGCY 5 cut(s) 20, 44, 79, 110, 119
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 3 cut(s) 21, 80, 169
DpnI GATC 3 cut(s) 91, 180, 204
DpnII GATC 3 cut(s) 89, 178, 202
EciI GGCGGA 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 81
FaiI YATR 3 cut(s) 155, 157, 217
FaqI GGGAC 1 cut(s) 154
FauI CCCGC 1 cut(s) 214
FauNDI CATATG 1 cut(s) 155
Fnu4HI GCNGC 1 cut(s) 117
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 41
FspI TGCGCA 1 cut(s) 115
GlaI GCGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 117
HhaI GCGC 1 cut(s) 116
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 1 cut(s) 35
HphI GGTGA 2 cut(s) 118, 161
Hpy188I TCNGA 3 cut(s) 24, 172, 202
Hpy188III TCNNGA 2 cut(s) 70, 82
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 3 cut(s) 21, 80, 169
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 89, 178, 202
LpnPI CCDG 4 cut(s) 24, 67, 105, 197
Lsp1109I GCAGC 1 cut(s) 128
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 149
MalI GATC 3 cut(s) 91, 180, 204
MboI GATC 3 cut(s) 89, 178, 202
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 1 cut(s) 195
MnlI CCTC 1 cut(s) 124
MseI TTAA 2 cut(s) 29, 102
MslI CAYNNNNRTG 1 cut(s) 214
MspA1I CMGCKG 1 cut(s) 119
MspCI CTTAAG 1 cut(s) 28
MwoI GCNNNNNNNGC 1 cut(s) 116
NdeI CATATG 1 cut(s) 155
NdeII GATC 3 cut(s) 89, 178, 202
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 149
NsbI TGCGCA 1 cut(s) 115
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 8
PsuI RGATCY 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 214
SaqAI TTAA 2 cut(s) 29, 102
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 3 cut(s) 89, 178, 202
Sau96I GGNCC 1 cut(s) 8
SduI GDGCHC 1 cut(s) 81
SetI ASST 7 cut(s) 13, 22, 46, 112, 121, 151, 216
SinI GGWCC 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 214
SmlI CTYRAG 2 cut(s) 15, 28
SmoI CTYRAG 2 cut(s) 15, 28
Sse9I AATT 1 cut(s) 195
SsiI CCGC 2 cut(s) 6, 207
SspI AATATT 1 cut(s) 61
SspMI CTAG 1 cut(s) 41
TasI AATT 1 cut(s) 195
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 2 cut(s) 29, 102
Tru9I TTAA 2 cut(s) 29, 102
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 2 cut(s) 87, 120
Vha464I CTTAAG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 8
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.