Rh2BG287600

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000565
Physical Location & Seq
Forward (+)
31786003 .. 31793815
7813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG287600.1

Sequence Viewer

Length: 504 bp
ATGAAAGCTTCATCTACGCCTAAAGAGTTGAGATCTTATCAAGGACGATCGATTCAGCTCTTGCAGGATCCGAAGCACCATACAAATTCAAAGGGACCTGCTCAAGCTCAGAACTTAAGAGATTCTCTAGCTGAAGCAAAGTTGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTTGCGCAACTGGTTTCACCGAGGAGAATGGGACTTTAGGTGACATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCCGCACAGTTGGATATAATTCATGACTTCTTTTTTTTTGACACTGCTGAAGTTTCCTCTGCAAACTACTCTGCTCGAGATTATGGAGTGAGGGCTGCGATGTTTGTCAGAGATGCTAAGGCTCTTTATCCACAACTTGTCATTTTTCCTTATGATTTTGAAGTGTATGAGGAGGTAGCTGATCAGTTCCATGACATTCTACATAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

167

Amino Acids

18.81

Weight (kDa)

5.3

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KARI_N PF07991 30 - 105 1.1e-06 Acetohydroxy acid isomeroreductase, NADPH-binding domain
IMS PF00817 113 - 166 7.5e-13 impB/mucB/samB family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000565)

Species Orthologous Gene IDs
pyrus_communis pycom10g08490
rosa_chinensis RchiOBHm_Chr1g0329691 RchiOBHm_Chr1g0332621 RchiOBHm_Chr2g0113681 RchiOBHm_Chr3g0494941 RchiOBHm_Chr4g0405101 RchiOBHm_Chr4g0432031 RchiOBHm_Chr6g0256671 RchiOBHm_Chr6g0271971
rosa_laevigata RLG00000000812 RLG00000014208 RLG00000019091 RLG00000023770 RLG00000024432 RLG00000030162
rosa_multiflora Rmu_sc0000173.1_g000039 Rmu_sc0000213.1_g000001 Rmu_sc0000580.1_g000059 Rmu_sc0001084.1_g000007 Rmu_sc0004755.1_g000037 Rmu_sc0005523.1_g000016 Rmu_sc0006670.1_g000025 Rmu_sc0021593.1_g000003
rosa_roxburghii Rroxscaffold_176G00431130 Rroxscaffold_1G00016960 Rroxscaffold_1G00025070 Rroxscaffold_1G00041460 Rroxscaffold_4G00294040 Rroxscaffold_6G00409240 Rroxscaffold_6G00423240 Rroxscaffold_6G00425100 Rroxscaffold_7G00185440 Rroxscaffold_7G00204100
rosa_rugosa Rorug03G0304000 Rorug03G0304000 Rorug06G0059000 Rorug06G0112900
rosa_samantha Rh1AG075200 Rh1AG157000 Rh1AG157100 Rh1AG161600 Rh1BG065800 Rh1BG094200 Rh2AG675300 Rh2BG242500 Rh2BG287600 Rh2BG319100 Rh2BG494800 Rh2BG686600 Rh2CG248000 Rh2CG288100 Rh2CG288200 Rh2CG649800 Rh2DG603100 Rh2DG700700 Rh3AG253500 Rh3BG236900 Rh3CG110600 Rh3CG318000 Rh3DG231400 Rh4CG141900 Rh5BG429900 Rh5CG251900 Rh5CG266000 Rh5CG350700 Rh5DG443300 Rh6AG032600 Rh6AG032700 Rh6BG408900 Rh6CG008400 Rh6DG009300 Rh7AG279000 Rh7BG261100 Rh7CG160900 Rh7CG298200 Rh7CG465100 Rh7CG502800 Rh7DG293900 Rh7DG471100 Rh7DG471300
rosa_wichuraiana Rw0G009600 Rw1G010610 Rw3G018660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 202
Acc36I ACCTGC 1 cut(s) 106
AciI CCGC 1 cut(s) 294
AclWI GGATC 5 cut(s) 62, 75, 171, 184, 284
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 2 cut(s) 153, 360
AflII CTTAAG 1 cut(s) 115
AgsI TTSAA 3 cut(s) 90, 145, 452
AluBI AGCT 6 cut(s) 8, 58, 107, 131, 197, 470
AluI AGCT 6 cut(s) 8, 58, 107, 131, 197, 470
AlwI GGATC 5 cut(s) 62, 75, 171, 184, 284
Ama87I CYCGRG 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 85
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 205, 248
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 1 cut(s) 95
BamHI GGATCC 2 cut(s) 67, 176
BanII GRGCYC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 373
BclI TGATCA 1 cut(s) 472
BfaI CTAG 1 cut(s) 128
BfrI CTTAAG 1 cut(s) 115
BfuAI ACCTGC 1 cut(s) 106
BglII AGATCT 1 cut(s) 32
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 3 cut(s) 69, 96, 178
BmsI GCATC 1 cut(s) 394
Bpu10I CCTNAGC 2 cut(s) 167, 408
BpuEI CTTGAG 1 cut(s) 87
Bsa29I ATCGAT 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 216
Bse1I ACTGG 1 cut(s) 211
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 216
BseMII CTCAG 3 cut(s) 122, 181, 270
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 2 cut(s) 233, 476
BseXI GCAGC 1 cut(s) 373
Bsh1285I CGRYCG 1 cut(s) 50
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 1 cut(s) 50
BsiHKCI CYCGRG 1 cut(s) 366
BslFI GGGAC 2 cut(s) 108, 241
BsmFI GGGAC 2 cut(s) 108, 241
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
BspACI CCGC 1 cut(s) 294
BspCNI CTCAG 3 cut(s) 121, 180, 269
BspDI ATCGAT 1 cut(s) 50
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 69, 96, 178
BspMI ACCTGC 1 cut(s) 106
BspPI GGATC 5 cut(s) 62, 75, 171, 184, 284
BspTI CTTAAG 1 cut(s) 115
BsrI ACTGG 1 cut(s) 211
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
Bst4CI ACNGT 1 cut(s) 300
BstAFI CTTAAG 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 4 cut(s) 108, 167, 256, 408
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 7 cut(s) 35, 50, 70, 179, 268, 292, 475
BstMBI GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
BstMCI CGRYCG 1 cut(s) 50
BstV1I GCAGC 1 cut(s) 373
BstX2I RGATCY 3 cut(s) 32, 67, 176
BstYI RGATCY 3 cut(s) 32, 67, 176
Bsu15I ATCGAT 1 cut(s) 50
BsuTUI ATCGAT 1 cut(s) 50
BtgZI GCGATG 1 cut(s) 404
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 2 cut(s) 333, 499
BveI ACCTGC 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 199
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 95
ClaI ATCGAT 1 cut(s) 50
CviAII CATG 2 cut(s) 314, 482
CviJI RGCY 9 cut(s) 8, 58, 107, 131, 166, 197, 386, 413, 470
CviKI_1 RGCY 9 cut(s) 8, 58, 107, 131, 166, 197, 386, 413, 470
DdeI CTNAG 4 cut(s) 108, 167, 256, 408
DpnI GATC 7 cut(s) 34, 49, 69, 178, 267, 291, 474
DpnII GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 95
Eco57I CTGAAG 2 cut(s) 153, 360
Eco88I CYCGRG 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 168
FaeI CATG 2 cut(s) 317, 485
FaqI GGGAC 2 cut(s) 108, 241
FatI CATG 2 cut(s) 313, 481
FauI CCCGC 1 cut(s) 301
FauNDI CATATG 1 cut(s) 242
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 1 cut(s) 387
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 1 cut(s) 128
FspI TGCGCA 1 cut(s) 202
GlaI GCGC 1 cut(s) 202
GluI GCNGC 1 cut(s) 387
HhaI GCGC 1 cut(s) 203
Hin1II CATG 2 cut(s) 317, 485
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HindIII AAGCTT 2 cut(s) 6, 195
HinfI GANTC 2 cut(s) 52, 122
HphI GGTGA 2 cut(s) 205, 248
Hpy188I TCNGA 5 cut(s) 72, 111, 259, 289, 401
Hpy188III TCNNGA 4 cut(s) 157, 169, 314, 368
HpyAV CCTTC 1 cut(s) 166
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 64, 353
HpyF3I CTNAG 4 cut(s) 108, 167, 256, 408
Hsp92II CATG 2 cut(s) 317, 485
HspAI GCGC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
LpnPI CCDG 4 cut(s) 50, 111, 154, 192
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 1 cut(s) 394
MaeI CTAG 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 236
MalI GATC 7 cut(s) 34, 49, 69, 178, 267, 291, 474
MboI GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
MflI RGATCY 3 cut(s) 32, 67, 176
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 3 cut(s) 85, 282, 309
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 5 cut(s) 211, 358, 375, 454, 457
MseI TTAA 2 cut(s) 116, 189
MspCI CTTAAG 1 cut(s) 115
NdeI CATATG 1 cut(s) 242
NdeII GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
NlaIII CATG 2 cut(s) 317, 485
NlaIV GGNNCC 3 cut(s) 69, 96, 178
NmuCI GTSAC 1 cut(s) 236
NsbI TGCGCA 1 cut(s) 202
PaeR7I CTCGAG 1 cut(s) 366
PagI TCATGA 1 cut(s) 313
PfeI GAWTC 2 cut(s) 52, 122
PkrI GCNGC 1 cut(s) 388
Ple19I CGATCG 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 95
Psp5II RGGWCCY 1 cut(s) 95
PspN4I GGNNCC 3 cut(s) 69, 96, 178
PspPI GGNCC 1 cut(s) 95
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 3 cut(s) 32, 67, 176
PvuI CGATCG 1 cut(s) 50
SaqAI TTAA 2 cut(s) 116, 189
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 7 cut(s) 32, 47, 67, 176, 265, 289, 472
Sau96I GGNCC 1 cut(s) 95
SduI GDGCHC 1 cut(s) 168
SetI ASST 9 cut(s) 10, 60, 100, 109, 133, 199, 238, 468, 472
SfaNI GCATC 1 cut(s) 394
Sfr274I CTCGAG 1 cut(s) 366
SinI GGWCC 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 366
SmlI CTYRAG 3 cut(s) 102, 115, 366
SmoI CTYRAG 3 cut(s) 102, 115, 366
Sse9I AATT 3 cut(s) 85, 282, 309
SsiI CCGC 1 cut(s) 294
SspI AATATT 1 cut(s) 148
SspMI CTAG 1 cut(s) 128
TaaI ACNGT 1 cut(s) 300
TaqI TCGA 2 cut(s) 50, 367
TasI AATT 3 cut(s) 85, 282, 309
TfiI GAWTC 2 cut(s) 52, 122
Tru1I TTAA 2 cut(s) 116, 189
Tru9I TTAA 2 cut(s) 116, 189
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 4 cut(s) 17, 174, 207, 302
TspRI CASTG 1 cut(s) 340
Vha464I CTTAAG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 1 cut(s) 85
XhoI CTCGAG 1 cut(s) 366
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.