Belongs to the formin-like family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 32, 137, 140, 184 |
| AclWI | GGATC | 1 cut(s) | 118 |
| AcoI | YGGCCR | 1 cut(s) | 214 |
| AcsI | RAATTY | 1 cut(s) | 269 |
| AfaI | GTAC | 1 cut(s) | 53 |
| AluBI | AGCT | 3 cut(s) | 193, 207, 244 |
| AluI | AGCT | 3 cut(s) | 193, 207, 244 |
| Alw26I | GTCTC | 2 cut(s) | 104, 241 |
| AlwI | GGATC | 1 cut(s) | 118 |
| AoxI | GGCC | 1 cut(s) | 214 |
| ApeKI | GCWGC | 2 cut(s) | 190, 207 |
| ApoI | RAATTY | 1 cut(s) | 269 |
| BalI | TGGCCA | 1 cut(s) | 216 |
| BanII | GRGCYC | 1 cut(s) | 266 |
| BbvI | GCAGC | 2 cut(s) | 194, 202 |
| BceAI | ACGGC | 1 cut(s) | 45 |
| BclI | TGATCA | 1 cut(s) | 69 |
| BcoDI | GTCTC | 2 cut(s) | 104, 241 |
| BfaI | CTAG | 1 cut(s) | 200 |
| BisI | GCNGC | 4 cut(s) | 137, 140, 191, 208 |
| BlsI | GCNGC | 4 cut(s) | 138, 141, 192, 209 |
| BmsI | GCATC | 1 cut(s) | 237 |
| BpuEI | CTTGAG | 1 cut(s) | 86 |
| BsaI | GGTCTC | 1 cut(s) | 241 |
| BsaJI | CCNNGG | 1 cut(s) | 211 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 102, 132 |
| Bse1I | ACTGG | 1 cut(s) | 59 |
| BseDI | CCNNGG | 1 cut(s) | 211 |
| BseMII | CTCAG | 2 cut(s) | 158, 208 |
| BseNI | ACTGG | 1 cut(s) | 59 |
| BseXI | GCAGC | 2 cut(s) | 194, 202 |
| BshFI | GGCC | 1 cut(s) | 216 |
| BsmAI | GTCTC | 2 cut(s) | 104, 241 |
| BsnI | GGCC | 1 cut(s) | 216 |
| Bso31I | GGTCTC | 1 cut(s) | 241 |
| Bsp1286I | GDGCHC | 1 cut(s) | 266 |
| Bsp143I | GATC | 4 cut(s) | 69, 77, 82, 123 |
| Bsp19I | CCATGG | 1 cut(s) | 211 |
| BspACI | CCGC | 4 cut(s) | 32, 137, 140, 184 |
| BspANI | GGCC | 1 cut(s) | 216 |
| BspCNI | CTCAG | 2 cut(s) | 157, 207 |
| BspPI | GGATC | 1 cut(s) | 118 |
| BspTNI | GGTCTC | 1 cut(s) | 241 |
| BsrI | ACTGG | 1 cut(s) | 59 |
| BssECI | CCNNGG | 1 cut(s) | 211 |
| BssMI | GATC | 4 cut(s) | 69, 77, 82, 123 |
| BssT1I | CCWWGG | 1 cut(s) | 211 |
| BstC8I | GCNNGC | 2 cut(s) | 188, 246 |
| BstDEI | CTNAG | 2 cut(s) | 144, 194 |
| BstDSI | CCRYGG | 1 cut(s) | 211 |
| BstKTI | GATC | 4 cut(s) | 72, 80, 85, 126 |
| BstMAI | GTCTC | 2 cut(s) | 104, 241 |
| BstMBI | GATC | 4 cut(s) | 69, 77, 82, 123 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 213 |
| BstV1I | GCAGC | 2 cut(s) | 194, 202 |
| BsuRI | GGCC | 1 cut(s) | 216 |
| BtgI | CCRYGG | 1 cut(s) | 211 |
| BtgZI | GCGATG | 1 cut(s) | 24 |
| Cac8I | GCNNGC | 2 cut(s) | 188, 246 |
| Csp6I | GTAC | 1 cut(s) | 52 |
| CviAII | CATG | 2 cut(s) | 16, 212 |
| CviJI | RGCY | 5 cut(s) | 193, 207, 216, 244, 264 |
| CviKI_1 | RGCY | 5 cut(s) | 193, 207, 216, 244, 264 |
| CviQI | GTAC | 1 cut(s) | 52 |
| DdeI | CTNAG | 2 cut(s) | 144, 194 |
| DpnI | GATC | 4 cut(s) | 71, 79, 84, 125 |
| DpnII | GATC | 4 cut(s) | 69, 77, 82, 123 |
| EaeI | YGGCCR | 1 cut(s) | 214 |
| EciI | GGCGGA | 1 cut(s) | 47 |
| Eco130I | CCWWGG | 1 cut(s) | 211 |
| Eco24I | GRGCYC | 1 cut(s) | 266 |
| Eco31I | GGTCTC | 1 cut(s) | 241 |
| EcoRI | GAATTC | 1 cut(s) | 269 |
| EcoT14I | CCWWGG | 1 cut(s) | 211 |
| EcoT38I | GRGCYC | 1 cut(s) | 266 |
| ErhI | CCWWGG | 1 cut(s) | 211 |
| FaeI | CATG | 2 cut(s) | 19, 215 |
| FaiI | YATR | 3 cut(s) | 17, 90, 213 |
| FatI | CATG | 2 cut(s) | 15, 211 |
| FbaI | TGATCA | 1 cut(s) | 69 |
| Fnu4HI | GCNGC | 4 cut(s) | 137, 140, 191, 208 |
| FriOI | GRGCYC | 1 cut(s) | 266 |
| Fsp4HI | GCNGC | 4 cut(s) | 137, 140, 191, 208 |
| FspBI | CTAG | 1 cut(s) | 200 |
| GluI | GCNGC | 4 cut(s) | 137, 140, 191, 208 |
| HaeIII | GGCC | 1 cut(s) | 216 |
| Hin1II | CATG | 2 cut(s) | 19, 215 |
| HinfI | GANTC | 2 cut(s) | 97, 155 |
| Hpy188I | TCNGA | 4 cut(s) | 82, 147, 268, 275 |
| Hpy188III | TCNNGA | 3 cut(s) | 73, 121, 127 |
| Hpy99I | CGWCG | 1 cut(s) | 31 |
| HpyCH4V | TGCA | 2 cut(s) | 109, 228 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 213 |
| HpyF3I | CTNAG | 2 cut(s) | 144, 194 |
| Hsp92II | CATG | 2 cut(s) | 19, 215 |
| Ksp22I | TGATCA | 1 cut(s) | 69 |
| Kzo9I | GATC | 4 cut(s) | 69, 77, 82, 123 |
| LmnI | GCTCC | 2 cut(s) | 23, 253 |
| LpnPI | CCDG | 2 cut(s) | 40, 200 |
| Lsp1109I | GCAGC | 2 cut(s) | 194, 202 |
| LweI | GCATC | 1 cut(s) | 237 |
| MaeI | CTAG | 1 cut(s) | 200 |
| MalI | GATC | 4 cut(s) | 71, 79, 84, 125 |
| MboI | GATC | 4 cut(s) | 69, 77, 82, 123 |
| MhlI | GDGCHC | 1 cut(s) | 266 |
| MlsI | TGGCCA | 1 cut(s) | 216 |
| MluCI | AATT | 2 cut(s) | 40, 269 |
| MluNI | TGGCCA | 1 cut(s) | 216 |
| MlyI | GAGTC | 1 cut(s) | 106 |
| MmeI | TCCRAC | 1 cut(s) | 275 |
| MnlI | CCTC | 3 cut(s) | 153, 161, 184 |
| Mox20I | TGGCCA | 1 cut(s) | 216 |
| MscI | TGGCCA | 1 cut(s) | 216 |
| Msp20I | TGGCCA | 1 cut(s) | 216 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 213 |
| NcoI | CCATGG | 1 cut(s) | 211 |
| NdeII | GATC | 4 cut(s) | 69, 77, 82, 123 |
| NlaIII | CATG | 2 cut(s) | 19, 215 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 164 |
| PfeI | GAWTC | 1 cut(s) | 155 |
| PkrI | GCNGC | 4 cut(s) | 138, 141, 192, 209 |
| PleI | GAGTC | 1 cut(s) | 105 |
| PpsI | GAGTC | 1 cut(s) | 105 |
| RsaI | GTAC | 1 cut(s) | 53 |
| RsaNI | GTAC | 1 cut(s) | 52 |
| SatI | GCNGC | 4 cut(s) | 137, 140, 191, 208 |
| Sau3AI | GATC | 4 cut(s) | 69, 77, 82, 123 |
| SchI | GAGTC | 1 cut(s) | 106 |
| SduI | GDGCHC | 1 cut(s) | 266 |
| SetI | ASST | 6 cut(s) | 95, 153, 176, 195, 209, 246 |
| SfaNI | GCATC | 1 cut(s) | 237 |
| SmlI | CTYRAG | 1 cut(s) | 101 |
| SmoI | CTYRAG | 1 cut(s) | 101 |
| Sse9I | AATT | 2 cut(s) | 40, 269 |
| SsiI | CCGC | 4 cut(s) | 32, 137, 140, 184 |
| SspMI | CTAG | 1 cut(s) | 200 |
| StyI | CCWWGG | 1 cut(s) | 211 |
| TaqI | TCGA | 2 cut(s) | 95, 153 |
| TasI | AATT | 2 cut(s) | 40, 269 |
| TatI | WGTACW | 1 cut(s) | 51 |
| TauI | GCSGC | 2 cut(s) | 139, 142 |
| TfiI | GAWTC | 1 cut(s) | 155 |
| TseI | GCWGC | 2 cut(s) | 190, 207 |
| XapI | RAATTY | 1 cut(s) | 269 |
| XspI | CTAG | 1 cut(s) | 200 |