Rh2BG242500

Ketol-acid reductoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000565
Physical Location & Seq
Forward (+)
24330254 .. 24331999
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG242500.1

Sequence Viewer

Length: 429 bp
ATGTTCGATTCTGCATCTACAACCGGAGAGCGTCTCTCTCGCCATTGCCGAACAGGCTCACAAGCAAATTCGAAAGTCCAGAGAGCTTCATCTACGCCTGAAGAGTTGAGATCTTATCAAGGACGATCGATTCAGCTCTTGCAGGATCCGAAGCACCATACAAATTCAAAGAACTTAAGAGATTCTCTAGTTGAAGCAAAGTCGAATATTGTGGTCAAGATTGGGCTCAGGAAGGGATCCCGTTCATTTAATGAAGCTCGCGCAGCTGGTTTCACCAAGGAGAATGGGACTTTAGGTGACATATGGGAAACAATCTCAGAGAGTGATCTTGTGTTGCTACTAATTTCTGATCCCGCACAGGCATATTACTTGGTGCTGTTCATGGAATTGTGGAGTCCCTTTTTAGAAGATACACCGACAGCGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
IlvC IlvN

Protein Analysis

142

Amino Acids

15.85

Weight (kDa)

6.31

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000565)

Species Orthologous Gene IDs
pyrus_communis pycom10g08490
rosa_chinensis RchiOBHm_Chr1g0329691 RchiOBHm_Chr1g0332621 RchiOBHm_Chr2g0113681 RchiOBHm_Chr3g0494941 RchiOBHm_Chr4g0405101 RchiOBHm_Chr4g0432031 RchiOBHm_Chr6g0256671 RchiOBHm_Chr6g0271971
rosa_laevigata RLG00000000812 RLG00000014208 RLG00000019091 RLG00000023770 RLG00000024432 RLG00000030162
rosa_multiflora Rmu_sc0000173.1_g000039 Rmu_sc0000213.1_g000001 Rmu_sc0000580.1_g000059 Rmu_sc0001084.1_g000007 Rmu_sc0004755.1_g000037 Rmu_sc0005523.1_g000016 Rmu_sc0006670.1_g000025 Rmu_sc0021593.1_g000003
rosa_roxburghii Rroxscaffold_176G00431130 Rroxscaffold_1G00016960 Rroxscaffold_1G00025070 Rroxscaffold_1G00041460 Rroxscaffold_4G00294040 Rroxscaffold_6G00409240 Rroxscaffold_6G00423240 Rroxscaffold_6G00425100 Rroxscaffold_7G00185440 Rroxscaffold_7G00204100
rosa_rugosa Rorug03G0304000 Rorug03G0304000 Rorug06G0059000 Rorug06G0112900
rosa_samantha Rh1AG075200 Rh1AG157000 Rh1AG157100 Rh1AG161600 Rh1BG065800 Rh1BG094200 Rh2AG675300 Rh2BG242500 Rh2BG287600 Rh2BG319100 Rh2BG494800 Rh2BG686600 Rh2CG248000 Rh2CG288100 Rh2CG288200 Rh2CG649800 Rh2DG603100 Rh2DG700700 Rh3AG253500 Rh3BG236900 Rh3CG110600 Rh3CG318000 Rh3DG231400 Rh4CG141900 Rh5BG429900 Rh5CG251900 Rh5CG266000 Rh5CG350700 Rh5DG443300 Rh6AG032600 Rh6AG032700 Rh6BG408900 Rh6CG008400 Rh6DG009300 Rh7AG279000 Rh7BG261100 Rh7CG160900 Rh7CG298200 Rh7CG465100 Rh7CG502800 Rh7DG293900 Rh7DG471100 Rh7DG471300
rosa_wichuraiana Rw0G009600 Rw1G010610 Rw3G018660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 261
AciI CCGC 2 cut(s) 354, 422
AclWI GGATC 5 cut(s) 140, 153, 231, 244, 344
AcsI RAATTY 2 cut(s) 67, 163
AcuI CTGAAG 1 cut(s) 120
AflII CTTAAG 1 cut(s) 175
AgsI TTSAA 2 cut(s) 168, 194
AluBI AGCT 4 cut(s) 86, 136, 257, 266
AluI AGCT 4 cut(s) 86, 136, 257, 266
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 5 cut(s) 140, 153, 231, 244, 344
ApeKI GCWGC 1 cut(s) 263
ApoI RAATTY 2 cut(s) 67, 163
AspLEI GCGC 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 265, 308
AsuII TTCGAA 1 cut(s) 71
BamHI GGATCC 2 cut(s) 145, 236
BanII GRGCYC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 275
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 1 cut(s) 188
BfrI CTTAAG 1 cut(s) 175
BglI GCCNNNNNGGC 1 cut(s) 54
BglII AGATCT 1 cut(s) 110
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
BmiI GGNNCC 2 cut(s) 147, 238
BmsI GCATC 1 cut(s) 23
BplI GAGNNNNNCTC 4 cut(s) 18, 50, 20, 52
Bpu10I CCTNAGC 1 cut(s) 227
Bpu14I TTCGAA 1 cut(s) 71
Bsa29I ATCGAT 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 23
Bse3DI GCAATG 1 cut(s) 43
BseCI ATCGAT 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 276
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 241, 330
BseXI GCAGC 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 261
Bsh1285I CGRYCG 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 2 cut(s) 301, 381
BsmAI GTCTC 1 cut(s) 38
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 2 cut(s) 301, 381
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 6 cut(s) 110, 125, 145, 236, 325, 349
BspACI CCGC 2 cut(s) 354, 422
BspCNI CTCAG 2 cut(s) 240, 329
BspDI ATCGAT 1 cut(s) 128
BspFNI CGCG 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 147, 238
BspPI GGATC 5 cut(s) 140, 153, 231, 244, 344
BspT104I TTCGAA 1 cut(s) 71
BspTI CTTAAG 1 cut(s) 175
BsrDI GCAATG 1 cut(s) 43
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 6 cut(s) 110, 125, 145, 236, 325, 349
BssT1I CCWWGG 1 cut(s) 276
Bst6I CTCTTC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 175
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 2 cut(s) 227, 316
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 6 cut(s) 113, 128, 148, 239, 328, 352
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 6 cut(s) 110, 125, 145, 236, 325, 349
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 2 cut(s) 54, 263
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 275
BstX2I RGATCY 3 cut(s) 110, 145, 236
BstYI RGATCY 3 cut(s) 110, 145, 236
Bsu15I ATCGAT 1 cut(s) 128
BsuTUI ATCGAT 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 259
CfoI GCGC 1 cut(s) 263
ClaI ATCGAT 1 cut(s) 128
CseI GACGC 1 cut(s) 20
CviAII CATG 1 cut(s) 382
CviJI RGCY 6 cut(s) 57, 86, 136, 226, 257, 266
CviKI_1 RGCY 6 cut(s) 57, 86, 136, 226, 257, 266
DdeI CTNAG 2 cut(s) 227, 316
DpnI GATC 6 cut(s) 112, 127, 147, 238, 327, 351
DpnII GATC 6 cut(s) 110, 125, 145, 236, 325, 349
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 1 cut(s) 385
FaiI YATR 5 cut(s) 159, 302, 304, 364, 383
FaqI GGGAC 2 cut(s) 301, 381
FatI CATG 1 cut(s) 381
FauI CCCGC 1 cut(s) 361
FauNDI CATATG 1 cut(s) 302
Fnu4HI GCNGC 1 cut(s) 264
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 1 cut(s) 188
GlaI GCGC 1 cut(s) 262
GluI GCNGC 1 cut(s) 264
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 20
HhaI GCGC 1 cut(s) 263
Hin1II CATG 1 cut(s) 385
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HinfI GANTC 4 cut(s) 8, 130, 182, 394
HpaII CCGG 1 cut(s) 24
HphI GGTGA 2 cut(s) 265, 308
Hpy188I TCNGA 3 cut(s) 150, 319, 349
Hpy188III TCNNGA 3 cut(s) 79, 217, 229
HpyAV CCTTC 1 cut(s) 226
HpyCH4V TGCA 2 cut(s) 14, 142
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 263
HpyF3I CTNAG 2 cut(s) 227, 316
Hsp92II CATG 1 cut(s) 385
HspAI GCGC 1 cut(s) 261
Kzo9I GATC 6 cut(s) 110, 125, 145, 236, 325, 349
LpnPI CCDG 8 cut(s) 37, 39, 92, 111, 128, 214, 252, 344
Lsp1109I GCAGC 1 cut(s) 275
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 1 cut(s) 296
MalI GATC 6 cut(s) 112, 127, 147, 238, 327, 351
MboI GATC 6 cut(s) 110, 125, 145, 236, 325, 349
MboII GAAGA 2 cut(s) 113, 419
MflI RGATCY 3 cut(s) 110, 145, 236
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 4 cut(s) 67, 163, 342, 386
MlyI GAGTC 1 cut(s) 403
MseI TTAA 2 cut(s) 176, 249
MspA1I CMGCKG 2 cut(s) 266, 422
MspCI CTTAAG 1 cut(s) 175
MspI CCGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 54, 263
NdeI CATATG 1 cut(s) 302
NdeII GATC 6 cut(s) 110, 125, 145, 236, 325, 349
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 147, 238
NmuCI GTSAC 1 cut(s) 296
NspV TTCGAA 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PfeI GAWTC 3 cut(s) 8, 130, 182
PkrI GCNGC 1 cut(s) 265
Ple19I CGATCG 1 cut(s) 128
PleI GAGTC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 402
PspN4I GGNNCC 2 cut(s) 147, 238
PsuI RGATCY 3 cut(s) 110, 145, 236
PvuI CGATCG 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 266
SaqAI TTAA 2 cut(s) 176, 249
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 6 cut(s) 110, 125, 145, 236, 325, 349
SchI GAGTC 1 cut(s) 403
SduI GDGCHC 1 cut(s) 228
SetI ASST 5 cut(s) 88, 138, 259, 268, 298
SfaNI GCATC 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 175
SmoI CTYRAG 1 cut(s) 175
Sse9I AATT 4 cut(s) 67, 163, 342, 386
SsiI CCGC 2 cut(s) 354, 422
SspI AATATT 1 cut(s) 208
SspMI CTAG 1 cut(s) 188
StyI CCWWGG 1 cut(s) 276
TaqI TCGA 4 cut(s) 6, 71, 128, 203
TasI AATT 4 cut(s) 67, 163, 342, 386
TfiI GAWTC 3 cut(s) 8, 130, 182
Tru1I TTAA 2 cut(s) 176, 249
Tru9I TTAA 2 cut(s) 176, 249
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 4 cut(s) 78, 234, 267, 370
Vha464I CTTAAG 1 cut(s) 175
XapI RAATTY 2 cut(s) 67, 163
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.