The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 172 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 260 |
| Acc36I | ACCTGC | 2 cut(s) | 172, 297 |
| AccII | CGCG | 1 cut(s) | 17 |
| AciI | CCGC | 1 cut(s) | 167 |
| AclWI | GGATC | 1 cut(s) | 117 |
| AfaI | GTAC | 1 cut(s) | 177 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 127 |
| Alw26I | GTCTC | 1 cut(s) | 307 |
| AlwI | GGATC | 1 cut(s) | 117 |
| AoxI | GGCC | 1 cut(s) | 41 |
| AsuHPI | GGTGA | 1 cut(s) | 153 |
| BbsI | GAAGAC | 1 cut(s) | 90 |
| BccI | CCATC | 1 cut(s) | 81 |
| BcoDI | GTCTC | 1 cut(s) | 307 |
| BfuAI | ACCTGC | 2 cut(s) | 172, 297 |
| BglII | AGATCT | 1 cut(s) | 202 |
| BpiI | GAAGAC | 1 cut(s) | 90 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 256 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 127 |
| BseDI | CCNNGG | 2 cut(s) | 105, 256 |
| BseGI | GGATG | 2 cut(s) | 73, 175 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 127 |
| BseMII | CTCAG | 1 cut(s) | 318 |
| Bsh1236I | CGCG | 1 cut(s) | 17 |
| BshFI | GGCC | 1 cut(s) | 43 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 127 |
| BsmAI | GTCTC | 1 cut(s) | 307 |
| BsnI | GGCC | 1 cut(s) | 43 |
| Bsp143I | GATC | 2 cut(s) | 109, 202 |
| Bsp68I | TCGCGA | 1 cut(s) | 17 |
| BspACI | CCGC | 1 cut(s) | 167 |
| BspANI | GGCC | 1 cut(s) | 43 |
| BspCNI | CTCAG | 1 cut(s) | 317 |
| BspFNI | CGCG | 1 cut(s) | 17 |
| BspMI | ACCTGC | 2 cut(s) | 172, 297 |
| BspPI | GGATC | 1 cut(s) | 117 |
| BssECI | CCNNGG | 2 cut(s) | 105, 256 |
| BssMI | GATC | 2 cut(s) | 109, 202 |
| BssT1I | CCWWGG | 1 cut(s) | 105 |
| Bst4CI | ACNGT | 1 cut(s) | 118 |
| BstDEI | CTNAG | 1 cut(s) | 304 |
| BstF5I | GGATG | 2 cut(s) | 73, 175 |
| BstFNI | CGCG | 1 cut(s) | 17 |
| BstKTI | GATC | 2 cut(s) | 112, 205 |
| BstMAI | GTCTC | 1 cut(s) | 307 |
| BstMBI | GATC | 2 cut(s) | 109, 202 |
| BstUI | CGCG | 1 cut(s) | 17 |
| BstV2I | GAAGAC | 1 cut(s) | 90 |
| BstX2I | RGATCY | 1 cut(s) | 202 |
| BstYI | RGATCY | 1 cut(s) | 202 |
| BsuRI | GGCC | 1 cut(s) | 43 |
| BtsCI | GGATG | 2 cut(s) | 73, 175 |
| BtuMI | TCGCGA | 1 cut(s) | 17 |
| BveI | ACCTGC | 2 cut(s) | 172, 297 |
| Csp6I | GTAC | 1 cut(s) | 176 |
| CviAII | CATG | 1 cut(s) | 251 |
| CviJI | RGCY | 2 cut(s) | 43, 272 |
| CviKI_1 | RGCY | 2 cut(s) | 43, 272 |
| CviQI | GTAC | 1 cut(s) | 176 |
| DdeI | CTNAG | 1 cut(s) | 304 |
| DpnI | GATC | 2 cut(s) | 111, 204 |
| DpnII | GATC | 2 cut(s) | 109, 202 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 260 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 260 |
| Eco130I | CCWWGG | 1 cut(s) | 105 |
| EcoT14I | CCWWGG | 1 cut(s) | 105 |
| ErhI | CCWWGG | 1 cut(s) | 105 |
| FaeI | CATG | 1 cut(s) | 254 |
| FaiI | YATR | 4 cut(s) | 55, 63, 237, 252 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 72, 104, 224, 256 |
| FatI | CATG | 1 cut(s) | 250 |
| FokI | GGATG | 2 cut(s) | 60, 182 |
| HaeIII | GGCC | 1 cut(s) | 43 |
| Hin1II | CATG | 1 cut(s) | 254 |
| HphI | GGTGA | 1 cut(s) | 153 |
| Hpy188I | TCNGA | 2 cut(s) | 144, 192 |
| Hpy188III | TCNNGA | 2 cut(s) | 16, 306 |
| HpyAV | CCTTC | 1 cut(s) | 239 |
| HpyCH4III | ACNGT | 1 cut(s) | 118 |
| HpyCH4V | TGCA | 1 cut(s) | 181 |
| HpyF3I | CTNAG | 1 cut(s) | 304 |
| Hsp92II | CATG | 1 cut(s) | 254 |
| Kzo9I | GATC | 2 cut(s) | 109, 202 |
| LmnI | GCTCC | 1 cut(s) | 308 |
| LpnPI | CCDG | 4 cut(s) | 25, 177, 291, 302 |
| MaeIII | GTNAC | 1 cut(s) | 159 |
| MalI | GATC | 2 cut(s) | 111, 204 |
| MboI | GATC | 2 cut(s) | 109, 202 |
| MboII | GAAGA | 2 cut(s) | 95, 197 |
| MfeI | CAATTG | 1 cut(s) | 265 |
| MflI | RGATCY | 1 cut(s) | 202 |
| MluCI | AATT | 1 cut(s) | 265 |
| MnlI | CCTC | 3 cut(s) | 76, 112, 251 |
| MseI | TTAA | 2 cut(s) | 231, 319 |
| MunI | CAATTG | 1 cut(s) | 265 |
| MvnI | CGCG | 1 cut(s) | 17 |
| NdeII | GATC | 2 cut(s) | 109, 202 |
| NlaIII | CATG | 1 cut(s) | 254 |
| NmuCI | GTSAC | 1 cut(s) | 159 |
| NruI | TCGCGA | 1 cut(s) | 17 |
| PaqCI | CACCTGC | 1 cut(s) | 172 |
| PsuI | RGATCY | 1 cut(s) | 202 |
| RruI | TCGCGA | 1 cut(s) | 17 |
| RsaI | GTAC | 1 cut(s) | 177 |
| RsaNI | GTAC | 1 cut(s) | 176 |
| SaqAI | TTAA | 2 cut(s) | 231, 319 |
| Sau3AI | GATC | 2 cut(s) | 109, 202 |
| SetI | ASST | 5 cut(s) | 90, 104, 166, 262, 291 |
| Sse9I | AATT | 1 cut(s) | 265 |
| SsiI | CCGC | 1 cut(s) | 167 |
| StyI | CCWWGG | 1 cut(s) | 105 |
| TaaI | ACNGT | 1 cut(s) | 118 |
| TaqII | GACCGA | 1 cut(s) | 270 |
| TasI | AATT | 1 cut(s) | 265 |
| TatI | WGTACW | 1 cut(s) | 175 |
| Tru1I | TTAA | 2 cut(s) | 231, 319 |
| Tru9I | TTAA | 2 cut(s) | 231, 319 |
| TseFI | GTSAC | 1 cut(s) | 159 |
| Tsp45I | GTSAC | 1 cut(s) | 159 |
| TspDTI | ATGAA | 2 cut(s) | 209, 264 |
| TspGWI | ACGGA | 1 cut(s) | 110 |