Rorug03G0327700

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000417
Physical Location & Seq
Reverse (-)
36277930 .. 36290373
12444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0327700.1

Sequence Viewer

Length: 4206 bp
ATGACGGCTCTGGATTCTACGGACCTCTTCCTGACTGCCCTAACCGCCTTTTCCCACCCACCCACATCCAGACCCAGACCCATCGAACTCTCTCTCCCTCCGATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTCGACGCATTCCGCTATGCCGGCGATTATTCCATCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGTCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATTGATGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCAGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGTTCCCGCCTCCAATGGAGACTTCCAGAGGTATGTTCACACCGGTGATTTTCGGTTTTCTGCTTCGATGAAGCTAGACCCTTTTCTCTCTAAGTTTTTGGGTTCAGATGCCGTTTTCTTGGACACAACTTATTGTAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTTGGAGGTGAACATCAAGTTTCTATGAAAAGCGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTATGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTCTGGGTTACGGAGACAGTGGGGTGTTTACGGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTTGTCCCTACTGGCTGGACTTATGAAGTGAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAACATTCAAGCTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACAGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACATGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATTGATGAAATGGCCAACAAAAAAGAGTTCTTGAGGGGTTTTCATCGTGGGACTACACAAGGAGGTGGGAAGGTTGACAGTGATGCCAAAGATTACACGATGGAGGGGCAGGATCAAGAGAAAAAGGCAACACCTTCAGATATTGATACTATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTTGTCTCCTCTCCAAGAACCTGATTCACAAACTCCAATATTGTTAACTGATGAAAAAGCAGAGGAAATTATTCAACAACTTCATGATTGTTTGCCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACAGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCCGTATCTGTATCCGAGACCAGCTCACTCTGTGACACAGCATCACCGACAAAAGCAGGTTCTGTTCATGCAAATATTGATGTTTCTTCAAGCCAAGCTCATATTTCACCCAACCCAAGAAATATTGTTAAAAGTGGCATTTCCCCTGGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAAAATTGAATTCGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTGCCTGACGTTACAGAAAGTGCAGAGATGGGATCAGTAGGTGAACAAAACCCTAAAGATAAAAAATTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTATGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAACTGTGGTATAGATAACTGTTCTTCTGTCCAAAAAAAAATTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACAGGGGTCATTACCAGATAACGTCGCTGCAACTTCTGTGTCACTACCACCAGAAAAATACAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTTGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTGCTTCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCCTGTCTCCTGATGATGTGCTGCCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCATGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGCATTGGGAGATCTTGGTGATGTTGCTCAAGCATGCCGGCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTGTTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAATCACGAAGGAAGTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTTGAAGCATACAATGTACTGCCCAATTTGGATTTGGTCGTTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAATACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAGCACTGAAGCTATTTGAAAATAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAATTCATAAACTAGTTGATGGGTCCATGCATGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCCGATGCAGTGACTTTCATACTGGATGCAGAGGTTGTTGGAGTTGATAGGAAGAATGGTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGAGGGAGCAGAGATGCATCAATAACGTTGGATTGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTACGATGAGAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCATGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGCGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGGTGAAAAGTTTTTATTCTGGGGACAAGATCTTGGCCAAAAAACCACCTTATTATAGAACAGCTGAGGTGCCTGATATGTGGTTTTCTCCTGAGCTTGTATGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCTGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

1401

Amino Acids

155.61

Weight (kDa)

6.46

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 267 - 370 3e-21 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 770 - 946 6.7e-42 DNA ligase N terminus
DNA_ligase_A_M PF01068 1016 - 1236 1.8e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1262 - 1369 2.4e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000417)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32770 FvH4_4g02520 FvH4_4g02520 FvH4_4g02530 FvH4_4g02550 FvH4_4g17730 FvH4_4g17730
rosa_chinensis RchiOBHm_Chr1g0318801 RchiOBHm_Chr4g0390011 RchiOBHm_Chr4g0390031 RchiOBHm_Chr4g0390041 RchiOBHm_Chr4g0390061 RchiOBHm_Chr4g0390091 RchiOBHm_Chr4g0390101 RchiOBHm_Chr4g0390141 RchiOBHm_Chr4g0390181 RchiOBHm_Chr4g0390191 RchiOBHm_Chr4g0390201 RchiOBHm_Chr4g0390231 RchiOBHm_Chr4g0390241 RchiOBHm_Chr4g0390251 RchiOBHm_Chr4g0390661 RchiOBHm_Chr4g0390711 RchiOBHm_Chr4g0390741 RchiOBHm_Chr4g0390771 RchiOBHm_Chr4g0390801 RchiOBHm_Chr5g0058271
rosa_laevigata RLG00000008685 RLG00000009910 RLG00000009930 RLG00000009932 RLG00000009933 RLG00000009934 RLG00000009935 RLG00000009937 RLG00000009938 RLG00000009940 RLG00000035208 RLG00000035209
rosa_multiflora Rmu_co8281525.1_g000001 Rmu_co8464925.1_g000001 Rmu_sc0000171.1_g000023 Rmu_sc0000171.1_g000027 Rmu_sc0000367.1_g000061 Rmu_sc0000679.1_g000027 Rmu_sc0001380.1_g000026 Rmu_sc0001380.1_g000030 Rmu_sc0001471.1_g000010 Rmu_sc0003643.1_g000007 Rmu_sc0004336.1_g000002 Rmu_sc0004336.1_g000011 Rmu_sc0004336.1_g000012 Rmu_sc0005003.1_g000007 Rmu_sc0005003.1_g000012 Rmu_sc0005003.1_g000016 Rmu_sc0005003.1_g000022 Rmu_sc0008322.1_g000002 Rmu_sc0011197.1_g000004 Rmu_sc0014620.1_g000006 Rmu_sc0014620.1_g000017 Rmu_sc0018617.1_g000002
rosa_roxburghii Rroxscaffold_1G00022270 Rroxscaffold_5G00336180 Rroxscaffold_5G00336220 Rroxscaffold_5G00336240 Rroxscaffold_5G00336250 Rroxscaffold_5G00336290 Rroxscaffold_5G00336310 Rroxscaffold_5G00336350 Rroxscaffold_5G00336370 Rroxscaffold_5G00336670 Rroxscaffold_5G00336700 Rroxscaffold_5G00336710
rosa_rugosa Rorug01G0020300 Rorug03G0327700 Rorug03G0327800 Rorug03G0328000 Rorug03G0328100 Rorug03G0328300 Rorug03G0328600 Rorug03G0328600 Rorug03G0331700 Rorug03G0331800 Rorug05G0312900
rosa_samantha Rh4DG024700 Rh4DG024900 Rh4DG025000 Rh4DG025200 Rh4DG025500 Rh4DG025900 Rh4DG026200 Rh4DG030200 Rh5DG407000
rosa_wichuraiana Rw4G002360 Rw4G002410 Rw4G002420 Rw4G002450 Rw4G002480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 300
Acc36I ACCTGC 3 cut(s) 1628, 2061, 2836
Acc65I GGTACC 1 cut(s) 2000
AccB1I GGYRCC 3 cut(s) 401, 2000, 3958
AccB7I CCANNNNNTGG 1 cut(s) 4064
AccBSI CCGCTC 2 cut(s) 281, 337
AccI GTMKAC 1 cut(s) 156
AccIII TCCGGA 1 cut(s) 2670
AciI CCGC 8 cut(s) 45, 166, 228, 279, 335, 435, 767, 3463
AclI AACGTT 1 cut(s) 3377
AclWI GGATC 4 cut(s) 1009, 1293, 1882, 2538
AcoI YGGCCR 4 cut(s) 869, 1185, 3133, 3924
AcsI RAATTY 8 cut(s) 572, 886, 1529, 1550, 1792, 2854, 3147, 3836
AcuI CTGAAG 6 cut(s) 1293, 1943, 2418, 2894, 3107, 3852
AcvI CACGTG 2 cut(s) 3347, 4064
AcyI GRCGYC 3 cut(s) 297, 384, 402
AdeI CACNNNGTG 1 cut(s) 1613
AfaI GTAC 9 cut(s) 1026, 2002, 2205, 2564, 2685, 2695, 2949, 3685, 3794
AfiI CCNNNNNNNGG 4 cut(s) 243, 1106, 2553, 4064
AflIII ACRYGT 1 cut(s) 3113
AgeI ACCGGT 1 cut(s) 470
AhlI ACTAGT 1 cut(s) 3157
AjnI CCWGG 5 cut(s) 397, 929, 1726, 3028, 4032
AjuI GAANNNNNNNTTGG 4 cut(s) 434, 466, 2475, 2507
AleI CACNNNNGTG 1 cut(s) 471
AloI GAACNNNNNNTCC 2 cut(s) 78, 110
Alw21I GWGCWC 4 cut(s) 1499, 2450, 2710, 3145
Alw44I GTGCAC 2 cut(s) 2446, 3141
AlwI GGATC 4 cut(s) 1009, 1293, 1882, 2538
AlwNI CAGNNNCTG 4 cut(s) 1643, 2696, 2927, 4139
Aor13HI TCCGGA 1 cut(s) 2670
AoxI GGCC 7 cut(s) 253, 869, 881, 1185, 1802, 3133, 3924
ApaLI GTGCAC 2 cut(s) 2446, 3141
ApeKI GCWGC 8 cut(s) 772, 2231, 2369, 2428, 2462, 2513, 2924, 4037
ApoI RAATTY 8 cut(s) 572, 886, 1529, 1550, 1792, 2854, 3147, 3836
ArsI GACNNNNNNTTYG 6 cut(s) 2602, 2634, 3410, 3442, 3835, 3867
AseI ATTAAT 1 cut(s) 3228
AsiGI ACCGGT 1 cut(s) 470
Asp700I GAANNNNTTC 4 cut(s) 1428, 2858, 3837, 4155
Asp718I GGTACC 1 cut(s) 2000
AspLEI GCGC 2 cut(s) 404, 2916
AspS9I GGNCC 4 cut(s) 22, 246, 1802, 3168
AsuII TTCGAA 1 cut(s) 2788
AvaII GGWCC 3 cut(s) 22, 246, 3168
BaeGI GKGCMC 2 cut(s) 2450, 3145
BaeI ACNNNNGTAYC 4 cut(s) 1323, 1356, 2782, 2815
BalI TGGCCA 4 cut(s) 871, 1187, 3135, 3926
BanI GGYRCC 3 cut(s) 401, 2000, 3958
BanII GRGCYC 2 cut(s) 1112, 1499
BarI GAAGNNNNNNTAC 4 cut(s) 3497, 3529, 4120, 4152
BbrPI CACGTG 2 cut(s) 3347, 4064
BbsI GAAGAC 2 cut(s) 278, 3406
Bbv12I GWGCWC 4 cut(s) 1499, 2450, 2710, 3145
BbvCI CCTCAGC 1 cut(s) 3954
BbvI GCAGC 8 cut(s) 759, 2218, 2356, 2415, 2449, 2525, 2936, 4024
BceAI ACGGC 7 cut(s) 21, 137, 241, 389, 524, 1438, 1568
BciT130I CCWGG 5 cut(s) 399, 931, 1728, 3030, 4034
BciVI GTATCC 1 cut(s) 1603
BclI TGATCA 1 cut(s) 1558
BcuI ACTAGT 1 cut(s) 3157
BfaI CTAG 6 cut(s) 503, 615, 1481, 2342, 3158, 4049
BfmI CTRYAG 5 cut(s) 1030, 1101, 2271, 2463, 2928
BfoI RGCGCY 2 cut(s) 405, 2917
BfuAI ACCTGC 3 cut(s) 1628, 2061, 2836
BfuI GTATCC 1 cut(s) 1603
BglII AGATCT 4 cut(s) 1483, 2578, 3220, 3918
BisI GCNGC 9 cut(s) 335, 773, 2232, 2370, 2429, 2463, 2514, 2925, 4038
BlsI GCNGC 9 cut(s) 336, 774, 2233, 2371, 2430, 2464, 2515, 2926, 4039
Bme1390I CCNGG 5 cut(s) 399, 931, 1728, 3030, 4034
Bme18I GGWCC 3 cut(s) 22, 246, 3168
BmgT120I GGNCC 4 cut(s) 22, 246, 1802, 3168
BmiI GGNNCC 8 cut(s) 248, 327, 403, 1111, 2002, 3027, 3169, 3960
BmrFI CCNGG 5 cut(s) 399, 931, 1728, 3030, 4034
BpiI GAAGAC 2 cut(s) 278, 3406
BplI GAGNNNNNCTC 2 cut(s) 1589, 1621
BpmI CTGGAG 3 cut(s) 2308, 2540, 4008
Bpu10I CCTNAGC 3 cut(s) 1498, 3954, 3981
Bpu14I TTCGAA 1 cut(s) 2788
BpuEI CTTGAG 3 cut(s) 1225, 2580, 2823
BsaAI YACGTR 3 cut(s) 2798, 3347, 4064
BsaHI GRCGYC 3 cut(s) 297, 384, 402
BsaI GGTCTC 2 cut(s) 236, 1592
BsaJI CCNNGG 5 cut(s) 250, 397, 930, 1726, 3528
BsaWI WCCGGW 2 cut(s) 470, 2670
BsaXI ACNNNNNCTCC 6 cut(s) 78, 108, 197, 227, 4006, 4036
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 1106, 2553, 4064
Bse118I RCCGGY 3 cut(s) 173, 470, 2604
Bse1I ACTGG 7 cut(s) 958, 1143, 2523, 2701, 3279, 3567, 4025
Bse3DI GCAATG 4 cut(s) 391, 1981, 2299, 2814
BseAI TCCGGA 1 cut(s) 2670
BseBI CCWGG 5 cut(s) 399, 931, 1728, 3030, 4034
BseDI CCNNGG 5 cut(s) 250, 397, 930, 1726, 3528
BseLI CCNNNNNNNGG 4 cut(s) 243, 1106, 2553, 4064
BseMI GCAATG 4 cut(s) 391, 1981, 2299, 2814
BseMII CTCAG 4 cut(s) 1512, 3945, 3972, 4178
BseNI ACTGG 7 cut(s) 958, 1143, 2523, 2701, 3279, 3567, 4025
BseRI GAGGAG 4 cut(s) 917, 1356, 2622, 2995
BseSI GKGCMC 2 cut(s) 2450, 3145
BseXI GCAGC 8 cut(s) 759, 2218, 2356, 2415, 2449, 2525, 2936, 4024
BseYI CCCAGC 1 cut(s) 1166
BsgI GTGCAG 3 cut(s) 1884, 2649, 4026
Bsh1285I CGRYCG 1 cut(s) 1540
BshFI GGCC 7 cut(s) 255, 871, 883, 1187, 1804, 3135, 3926
BshNI GGYRCC 3 cut(s) 401, 2000, 3958
BshTI ACCGGT 1 cut(s) 470
BsiEI CGRYCG 1 cut(s) 1540
BsiHKAI GWGCWC 4 cut(s) 1499, 2450, 2710, 3145
BsiSI CCGG 4 cut(s) 174, 471, 2605, 2671
BslFI GGGAC 7 cut(s) 232, 932, 1237, 1520, 2548, 3734, 3926
BslI CCNNNNNNNGG 4 cut(s) 243, 1106, 2553, 4064
BsmBI CGTCTC 1 cut(s) 313
BsmFI GGGAC 7 cut(s) 232, 932, 1237, 1520, 2548, 3734, 3926
BsmI GAATGC 1 cut(s) 161
BsnI GGCC 7 cut(s) 255, 871, 883, 1187, 1804, 3135, 3926
Bso31I GGTCTC 2 cut(s) 236, 1592
Bsp119I TTCGAA 1 cut(s) 2788
Bsp1286I GDGCHC 5 cut(s) 1112, 1499, 2450, 2710, 3145
Bsp13I TCCGGA 1 cut(s) 2670
Bsp1407I TGTACA 3 cut(s) 2203, 2562, 2683
BspACI CCGC 8 cut(s) 45, 166, 228, 279, 335, 435, 767, 3463
BspANI GGCC 7 cut(s) 255, 871, 883, 1187, 1804, 3135, 3926
BspCNI CTCAG 4 cut(s) 1511, 3946, 3973, 4177
BspEI TCCGGA 1 cut(s) 2670
BspHI TCATGA 3 cut(s) 1441, 1555, 2982
BspLI GGNNCC 8 cut(s) 248, 327, 403, 1111, 2002, 3027, 3169, 3960
BspMAI CTGCAG 1 cut(s) 2467
BspMI ACCTGC 3 cut(s) 1628, 2061, 2836
BspPI GGATC 4 cut(s) 1009, 1293, 1882, 2538
BspT104I TTCGAA 1 cut(s) 2788
BspT107I GGYRCC 3 cut(s) 401, 2000, 3958
BspTNI GGTCTC 2 cut(s) 236, 1592
BsrBI CCGCTC 2 cut(s) 281, 337
BsrDI GCAATG 4 cut(s) 391, 1981, 2299, 2814
BsrFI RCCGGY 3 cut(s) 173, 470, 2604
BsrGI TGTACA 3 cut(s) 2203, 2562, 2683
BsrI ACTGG 7 cut(s) 958, 1143, 2523, 2701, 3279, 3567, 4025
BssAI RCCGGY 3 cut(s) 173, 470, 2604
BssECI CCNNGG 5 cut(s) 250, 397, 930, 1726, 3528
BssNI GRCGYC 3 cut(s) 297, 384, 402
BssT1I CCWWGG 2 cut(s) 250, 3528
Bst2UI CCWGG 5 cut(s) 399, 931, 1728, 3030, 4034
Bst6I CTCTTC 2 cut(s) 32, 316
BstACI GRCGYC 3 cut(s) 297, 384, 402
BstAPI GCANNNNNTGC 1 cut(s) 3554
BstAUI TGTACA 3 cut(s) 2203, 2562, 2683
BstBAI YACGTR 3 cut(s) 2798, 3347, 4064
BstBI TTCGAA 1 cut(s) 2788
BstDEI CTNAG 6 cut(s) 519, 1058, 1498, 3954, 3981, 4164
BstH2I RGCGCY 2 cut(s) 405, 2917
BstHHI GCGC 2 cut(s) 404, 2916
BstMCI CGRYCG 1 cut(s) 1540
BstMWI GCNNNNNNNGC 6 cut(s) 44, 158, 174, 343, 3469, 3554
BstNI CCWGG 5 cut(s) 399, 931, 1728, 3030, 4034
BstNSI RCATGY 9 cut(s) 839, 1142, 2286, 2306, 2532, 2604, 3117, 3179, 3816
BstSCI CCNGG 5 cut(s) 397, 929, 1726, 3028, 4032
BstSFI CTRYAG 5 cut(s) 1030, 1101, 2271, 2463, 2928
BstSLI GKGCMC 2 cut(s) 2450, 3145
BstSNI TACGTA 1 cut(s) 2798
BstV1I GCAGC 8 cut(s) 759, 2218, 2356, 2415, 2449, 2525, 2936, 4024
BstV2I GAAGAC 2 cut(s) 278, 3406
BstX2I RGATCY 6 cut(s) 1014, 1483, 2543, 2578, 3220, 3918
BstYI RGATCY 6 cut(s) 1014, 1483, 2543, 2578, 3220, 3918
BsuI GTATCC 1 cut(s) 1603
BsuRI GGCC 7 cut(s) 255, 871, 883, 1187, 1804, 3135, 3926
BtsI GCAGTG 2 cut(s) 2710, 3267
BveI ACCTGC 3 cut(s) 1628, 2061, 2836
CaiI CAGNNNCTG 4 cut(s) 1643, 2696, 2927, 4139
CciI TCATGA 3 cut(s) 1441, 1555, 2982
CfoI GCGC 2 cut(s) 404, 2916
Cfr10I RCCGGY 3 cut(s) 173, 470, 2604
Cfr13I GGNCC 4 cut(s) 22, 246, 1802, 3168
CseI GACGC 5 cut(s) 167, 392, 2534, 3394, 3835
Csp6I GTAC 9 cut(s) 1025, 2001, 2204, 2563, 2684, 2694, 2948, 3684, 3793
CspAI ACCGGT 1 cut(s) 470
CviQI GTAC 9 cut(s) 1025, 2001, 2204, 2563, 2684, 2694, 2948, 3684, 3793
DdeI CTNAG 6 cut(s) 519, 1058, 1498, 3954, 3981, 4164
DinI GGCGCC 1 cut(s) 403
DraIII CACNNNGTG 1 cut(s) 1613
EaeI YGGCCR 4 cut(s) 869, 1185, 3133, 3924
Eam1104I CTCTTC 2 cut(s) 32, 316
EarI CTCTTC 2 cut(s) 32, 316
Ecl136II GAGCTC 1 cut(s) 1497
Eco105I TACGTA 1 cut(s) 2798
Eco130I CCWWGG 2 cut(s) 250, 3528
Eco147I AGGCCT 1 cut(s) 255
Eco24I GRGCYC 2 cut(s) 1112, 1499
Eco31I GGTCTC 2 cut(s) 236, 1592
Eco47I GGWCC 3 cut(s) 22, 246, 3168
Eco53kI GAGCTC 1 cut(s) 1497
Eco57I CTGAAG 6 cut(s) 1293, 1943, 2418, 2894, 3107, 3852
Eco72I CACGTG 2 cut(s) 3347, 4064
EcoICRI GAGCTC 1 cut(s) 1497
EcoRI GAATTC 2 cut(s) 1792, 2854
EcoRII CCWGG 5 cut(s) 397, 929, 1726, 3028, 4032
EcoT14I CCWWGG 2 cut(s) 250, 3528
EcoT22I ATGCAT 5 cut(s) 2284, 2572, 3177, 3370, 3613
EcoT38I GRGCYC 2 cut(s) 1112, 1499
EgeI GGCGCC 1 cut(s) 403
EheI GGCGCC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 250, 3528
Esp3I CGTCTC 1 cut(s) 313
FaqI GGGAC 7 cut(s) 232, 932, 1237, 1520, 2548, 3734, 3926
FauI CCCGC 1 cut(s) 442
FauNDI CATATG 1 cut(s) 685
FbaI TGATCA 1 cut(s) 1558
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 9 cut(s) 335, 773, 2232, 2370, 2429, 2463, 2514, 2925, 4038
FriOI GRGCYC 2 cut(s) 1112, 1499
Fsp4HI GCNGC 9 cut(s) 335, 773, 2232, 2370, 2429, 2463, 2514, 2925, 4038
FspBI CTAG 6 cut(s) 503, 615, 1481, 2342, 3158, 4049
GlaI GCGC 2 cut(s) 403, 2915
GluI GCNGC 9 cut(s) 335, 773, 2232, 2370, 2429, 2463, 2514, 2925, 4038
GsaI CCCAGC 1 cut(s) 1170
GsuI CTGGAG 3 cut(s) 2308, 2540, 4008
HaeII RGCGCY 2 cut(s) 405, 2917
HaeIII GGCC 7 cut(s) 255, 871, 883, 1187, 1804, 3135, 3926
HapII CCGG 4 cut(s) 174, 471, 2605, 2671
HgaI GACGC 5 cut(s) 167, 392, 2534, 3394, 3835
HhaI GCGC 2 cut(s) 404, 2916
Hin1I GRCGYC 3 cut(s) 297, 384, 402
Hin6I GCGC 2 cut(s) 402, 2914
HinP1I GCGC 2 cut(s) 402, 2914
HincII GTYRAC 6 cut(s) 157, 1249, 1404, 1762, 3204, 3397
HindII GTYRAC 6 cut(s) 157, 1249, 1404, 1762, 3204, 3397
HindIII AAGCTT 3 cut(s) 2906, 3105, 3451
HpaI GTTAAC 2 cut(s) 1404, 1762
HpaII CCGG 4 cut(s) 174, 471, 2605, 2671
Hpy99I CGWCG 3 cut(s) 158, 161, 2231
HpyCH4IV ACGT 9 cut(s) 297, 306, 1068, 1851, 2226, 2797, 3346, 3377, 4063
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 158, 174, 343, 3469, 3554
HpyF3I CTNAG 6 cut(s) 519, 1058, 1498, 3954, 3981, 4164
HpySE526I ACGT 9 cut(s) 297, 306, 1068, 1851, 2226, 2797, 3346, 3377, 4063
Hsp92I GRCGYC 3 cut(s) 297, 384, 402
HspAI GCGC 2 cut(s) 402, 2914
KasI GGCGCC 1 cut(s) 401
Kpn2I TCCGGA 1 cut(s) 2670
KpnI GGTACC 1 cut(s) 2004
KroI GCCGGC 2 cut(s) 173, 2604
KroNI GCCGGC 2 cut(s) 175, 2606
Ksp22I TGATCA 1 cut(s) 1558
KspAI GTTAAC 2 cut(s) 1404, 1762
LmnI GCTCC 9 cut(s) 272, 1115, 1494, 2092, 2289, 2311, 2804, 3357, 3758
Lsp1109I GCAGC 8 cut(s) 759, 2218, 2356, 2415, 2449, 2525, 2936, 4024
MaeI CTAG 6 cut(s) 503, 615, 1481, 2342, 3158, 4049
MaeII ACGT 9 cut(s) 297, 306, 1068, 1851, 2226, 2797, 3346, 3377, 4063
MbiI CCGCTC 2 cut(s) 281, 337
MfeI CAATTG 2 cut(s) 241, 3753
MflI RGATCY 6 cut(s) 1014, 1483, 2543, 2578, 3220, 3918
MhlI GDGCHC 5 cut(s) 1112, 1499, 2450, 2710, 3145
MlsI TGGCCA 4 cut(s) 871, 1187, 3135, 3926
MluNI TGGCCA 4 cut(s) 871, 1187, 3135, 3926
Mly113I GGCGCC 1 cut(s) 402
MlyI GAGTC 1 cut(s) 3209
MmeI TCCRAC 4 cut(s) 613, 2148, 3271, 3360
Mox20I TGGCCA 4 cut(s) 871, 1187, 3135, 3926
Mph1103I ATGCAT 5 cut(s) 2284, 2572, 3177, 3370, 3613
MroI TCCGGA 1 cut(s) 2670
MroNI GCCGGC 2 cut(s) 173, 2604
MroXI GAANNNNTTC 4 cut(s) 1428, 2858, 3837, 4155
MscI TGGCCA 4 cut(s) 871, 1187, 3135, 3926
MslI CAYNNNNRTG 2 cut(s) 471, 2475
Msp20I TGGCCA 4 cut(s) 871, 1187, 3135, 3926
MspA1I CMGCKG 2 cut(s) 2927, 3953
MspI CCGG 4 cut(s) 174, 471, 2605, 2671
MspR9I CCNGG 5 cut(s) 399, 931, 1728, 3030, 4034
MunI CAATTG 2 cut(s) 241, 3753
Mva1269I GAATGC 1 cut(s) 161
MvaI CCWGG 5 cut(s) 399, 931, 1728, 3030, 4034
MwoI GCNNNNNNNGC 6 cut(s) 44, 158, 174, 343, 3469, 3554
NaeI GCCGGC 2 cut(s) 175, 2606
NarI GGCGCC 1 cut(s) 402
NdeI CATATG 1 cut(s) 685
NgoMIV GCCGGC 2 cut(s) 173, 2604
NlaIV GGNNCC 8 cut(s) 248, 327, 403, 1111, 2002, 3027, 3169, 3960
NmuCI GTSAC 8 cut(s) 1462, 1522, 1613, 1921, 2244, 2506, 3262, 4102
NsiI ATGCAT 5 cut(s) 2284, 2572, 3177, 3370, 3613
NspI RCATGY 9 cut(s) 839, 1142, 2286, 2306, 2532, 2604, 3117, 3179, 3816
NspV TTCGAA 1 cut(s) 2788
OliI CACNNNNGTG 1 cut(s) 471
PaeI GCATGC 2 cut(s) 2286, 2604
PagI TCATGA 3 cut(s) 1441, 1555, 2982
PceI AGGCCT 1 cut(s) 255
PciI ACATGT 1 cut(s) 3113
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 2794
PctI GAATGC 1 cut(s) 161
PdiI GCCGGC 2 cut(s) 175, 2606
PdmI GAANNNNTTC 4 cut(s) 1428, 2858, 3837, 4155
PflMI CCANNNNNTGG 1 cut(s) 4064
PfoI TCCNGGA 1 cut(s) 3028
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 9 cut(s) 336, 774, 2233, 2371, 2430, 2464, 2515, 2926, 4039
Ple19I CGATCG 1 cut(s) 1540
PleI GAGTC 1 cut(s) 3208
PluTI GGCGCC 1 cut(s) 405
PmaCI CACGTG 2 cut(s) 3347, 4064
PmlI CACGTG 2 cut(s) 3347, 4064
PpsI GAGTC 1 cut(s) 3208
Ppu21I YACGTR 3 cut(s) 2798, 3347, 4064
PscI ACATGT 1 cut(s) 3113
PshBI ATTAAT 1 cut(s) 3228
Psp124BI GAGCTC 1 cut(s) 1499
Psp1406I AACGTT 1 cut(s) 3377
Psp6I CCWGG 5 cut(s) 397, 929, 1726, 3028, 4032
PspCI CACGTG 2 cut(s) 3347, 4064
PspFI CCCAGC 1 cut(s) 1166
PspGI CCWGG 5 cut(s) 397, 929, 1726, 3028, 4032
PspN4I GGNNCC 8 cut(s) 248, 327, 403, 1111, 2002, 3027, 3169, 3960
PspPI GGNCC 4 cut(s) 22, 246, 1802, 3168
PstI CTGCAG 1 cut(s) 2467
PstNI CAGNNNCTG 4 cut(s) 1643, 2696, 2927, 4139
PsuI RGATCY 6 cut(s) 1014, 1483, 2543, 2578, 3220, 3918
PvuI CGATCG 1 cut(s) 1540
PvuII CAGCTG 2 cut(s) 2927, 3953
RsaI GTAC 9 cut(s) 1026, 2002, 2205, 2564, 2685, 2695, 2949, 3685, 3794
RsaNI GTAC 9 cut(s) 1025, 2001, 2204, 2563, 2684, 2694, 2948, 3684, 3793
RseI CAYNNNNRTG 2 cut(s) 471, 2475
SacI GAGCTC 1 cut(s) 1499
SalI GTCGAC 1 cut(s) 155
SatI GCNGC 9 cut(s) 335, 773, 2232, 2370, 2429, 2463, 2514, 2925, 4038
Sau96I GGNCC 4 cut(s) 22, 246, 1802, 3168
SchI GAGTC 1 cut(s) 3209
ScrFI CCNGG 5 cut(s) 399, 931, 1728, 3030, 4034
SduI GDGCHC 5 cut(s) 1112, 1499, 2450, 2710, 3145
SfcI CTRYAG 5 cut(s) 1030, 1101, 2271, 2463, 2928
SfoI GGCGCC 1 cut(s) 403
SfuI TTCGAA 1 cut(s) 2788
SgrAI CRCCGGYG 1 cut(s) 470
SgrDI CGTCGACG 1 cut(s) 155
SinI GGWCC 3 cut(s) 22, 246, 3168
SmiMI CAYNNNNRTG 2 cut(s) 471, 2475
SmlI CTYRAG 3 cut(s) 1204, 2595, 2838
SmoI CTYRAG 3 cut(s) 1204, 2595, 2838
SnaBI TACGTA 1 cut(s) 2798
SpeI ACTAGT 1 cut(s) 3157
SphI GCATGC 2 cut(s) 2286, 2604
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 8 cut(s) 45, 166, 228, 279, 335, 435, 767, 3463
SspDI GGCGCC 1 cut(s) 401
SspI AATATT 3 cut(s) 1398, 1657, 1705
SspMI CTAG 6 cut(s) 503, 615, 1481, 2342, 3158, 4049
SstI GAGCTC 1 cut(s) 1499
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 5 cut(s) 397, 929, 1726, 3028, 4032
StyI CCWWGG 2 cut(s) 250, 3528
TaiI ACGT 9 cut(s) 300, 309, 1071, 1854, 2229, 2800, 3349, 3380, 4066
TatI WGTACW 4 cut(s) 2203, 2562, 2683, 2947
TauI GCSGC 1 cut(s) 337
TseFI GTSAC 8 cut(s) 1462, 1522, 1613, 1921, 2244, 2506, 3262, 4102
TseI GCWGC 8 cut(s) 772, 2231, 2369, 2428, 2462, 2513, 2924, 4037
Tsp45I GTSAC 8 cut(s) 1462, 1522, 1613, 1921, 2244, 2506, 3262, 4102
TspGWI ACGGA 3 cut(s) 35, 804, 824
Van91I CCANNNNNTGG 1 cut(s) 4064
VneI GTGCAC 2 cut(s) 2446, 3141
VpaK11BI GGWCC 3 cut(s) 22, 246, 3168
VspI ATTAAT 1 cut(s) 3228
XapI RAATTY 8 cut(s) 572, 886, 1529, 1550, 1792, 2854, 3147, 3836
XceI RCATGY 9 cut(s) 839, 1142, 2286, 2306, 2532, 2604, 3117, 3179, 3816
XcmI CCANNNNNNNNNTGG 2 cut(s) 2962, 3759
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 4 cut(s) 1428, 2858, 3837, 4155
XspI CTAG 6 cut(s) 503, 615, 1481, 2342, 3158, 4049
ZraI GACGTC 1 cut(s) 298
Zsp2I ATGCAT 5 cut(s) 2284, 2572, 3177, 3370, 3613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.