Rorug05G0218700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000636
Physical Location & Seq
Reverse (-)
22477496 .. 22478805
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0218700.1

Sequence Viewer

Length: 1218 bp
ATGAAAACTCTTCAGCAAAAGTTACAAACGTTGCCTGCTCTTGCCCAGTCCATCCGCTCAATTGAGCTCCCAGAGGAGCCCTCCCCTGATCAGGTTATTTGGTCTGAAACGGCGTCAGGGTCATTGACATCCAGTGATGCTTACAACGCCTTGGATCCTTCTATGCCTGGCCCTTCATGGTGCAAGCACATTTGGCATGCTGCAATACAACCTAGAAAGAGTGTCACCACTTGGAAAGTCTTGCATGGAAAAATTCAAACAGATGATTTTCTTCAGAAACGGGGTACAAGTCTGTGTTCTAGATGCCTCCTTTGTGGGACAAATTCTGAATCACACATGCATCTTTTTCAGACTTGTGAGATCGCCAGTTCATTATGGCACTGGCTCCACCATCTCTTCAGATTGCGGTTCCCCCCAGGTGCGTCGCTCCATGATTTTTTCCTTGATAATTTGCTATCCCTACTCGATTATTCCTCCAAGTTATTGTGGTACATAGCGGTCTGCAACCTCCTTTGGTGCATCTGGCAAGAAAGGAACAAAAGAAAGTTTGATGGGGGAGCTTTTTGTCTATCAAGATTTAAGCAGTTTTTCACCCTATCACTAAGGGAATCTGCAGGGTTAATATTTGCCTCATCCTCCCTGGCATCCTCTGTAACCCCCATTTTCGGCCTATTGGGCATCTCCCCCCTCCGCCCCATGGCACCTCGTTATATTCCAGTCACATGGACTCCCCCGCCAGAGAATTGGTTCAAAGCAAATTCAGATGGCTCTTTTCGTGATCCCAATCATGCCGGCTTTGGAGGCATTTTTAGAAACAGTGATGCAGCTTTCTTAGGTGCCTTCTCTTGTAAAGCAATTGTGCCATGGGCCATTGACACTGAGTTATTAGCAGTAATGGCGGTTGTTAAAGTGGCAAGTGATCGTAACTGGTTTCCTCTATGGTTAGAGACCGATTCAATGTTGGTTATGCACTATTTTAACAATCCATCCAGGGTGCCTTGGCATCTTCGTACTAGATGGAAAAACTGTGTTGCTTCGATTCGCCAATTGCAAGTGCACATCTCCCATATCTATAGAGAAGGGAACCGTGTGGCCGACAAGCTGGCTAATTTTGGAGCCACGAATGACAATCCAACTTGGTGGGATTCAATTCCGCCTTTCCTGATTAGCGCTTTTGGTCATGACTATTCAGCCCAGACGGACTACCGGTTTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

46.12

Weight (kDa)

8.77

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 43 - 126 2.2e-21 zinc-binding in reverse transcriptase
RVT_3 PF13456 253 - 371 1.5e-14 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000636)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36290 FvH4_5g27770 FvH4_5g29860 FvH4_5g37342 FvH4_5g37877 FvH4_5g37880 FvH4_5g37900 FvH4_6g40020
rosa_chinensis RchiOBHm_Chr7g0227221 RchiOBHm_Chr7g0239011
rosa_multiflora Rmu_co8202712.1_g000001 Rmu_sc0000477.1_g000006 Rmu_sc0001047.1_g000050 Rmu_sc0001371.1_g000021 Rmu_sc0002449.1_g000018 Rmu_sc0002988.1_g000003 Rmu_sc0003089.1_g000031 Rmu_sc0004062.1_g000013 Rmu_sc0004132.1_g000001 Rmu_sc0004277.1_g000045 Rmu_sc0004567.1_g000017 Rmu_sc0005327.1_g000024 Rmu_sc0005651.1_g000005 Rmu_sc0006669.1_g000014 Rmu_sc0018004.1_g000002 Rmu_sc0018978.1_g000001 Rmu_sc0021209.1_g000001 Rmu_sc0034369.1_g000001 Rmu_ssc0000398.1_g000019
rosa_roxburghii Rroxscaffold_1G00004410 Rroxscaffold_3G00220090 Rroxscaffold_3G00221380 Rroxscaffold_3G00222090 Rroxscaffold_3G00223100 Rroxscaffold_6G00400160 Rroxscaffold_7G00204490 Rroxscaffold_7G00204500
rosa_rugosa Rorug02G0147900 Rorug03G0117000 Rorug03G0194000 Rorug04G0112500 Rorug05G0218600 Rorug05G0218700 Rorug05G0218800 Rorug05G0218900 Rorug05G0219000 Rorug05G0219000 Rorug05G0219000 Rorug05G0219100 Rorug05G0220000 Rorug05G0574900 Rorug07G0311900 Rorug07G0312000 Rorug07G0324500 Rorug07G0324600
rosa_samantha Rh3BG280100 Rh4BG071800 Rh5BG306100 Rh5BG308200 Rh5BG313000 Rh5BG335200 Rh5BG532100 Rh5BG532200 Rh5CG556100 Rh5DG315400 Rh5DG318300 Rh5DG318400 Rh5DG347500 Rh6CG116800 Rh7BG420800 Rh7BG437600 Rh7BG452200 Rh7CG484800 Rh7CG496900 Rh7DG453200 Rh7DG464300
rosa_wichuraiana Rw7G039600 Rw7G041070

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 700, 836, 994
AccBSI CCGCTC 1 cut(s) 57
AciI CCGC 7 cut(s) 55, 406, 497, 691, 734, 899, 1154
AclI AACGTT 1 cut(s) 29
AclWI GGATC 3 cut(s) 149, 162, 773
AcoI YGGCCR 1 cut(s) 1092
AcsI RAATTY 3 cut(s) 252, 322, 757
AcuI CTGAAG 2 cut(s) 257, 382
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 3 cut(s) 286, 491, 1012
AfeI AGCGCT 1 cut(s) 1171
AfiI CCNNNNNNNGG 3 cut(s) 91, 665, 697
AgeI ACCGGT 1 cut(s) 1206
AgsI TTSAA 4 cut(s) 257, 751, 957, 1149
AjnI CCWGG 4 cut(s) 166, 415, 639, 989
AluBI AGCT 4 cut(s) 67, 560, 827, 1102
AluI AGCT 4 cut(s) 67, 560, 827, 1102
Alw21I GWGCWC 2 cut(s) 69, 1059
Alw26I GTCTC 1 cut(s) 941
Alw44I GTGCAC 1 cut(s) 1055
AlwI GGATC 3 cut(s) 149, 162, 773
Aor51HI AGCGCT 1 cut(s) 1171
AoxI GGCC 4 cut(s) 169, 667, 867, 1092
ApaLI GTGCAC 1 cut(s) 1055
ApeKI GCWGC 2 cut(s) 200, 824
ApoI RAATTY 3 cut(s) 252, 322, 757
AsiGI ACCGGT 1 cut(s) 1206
Asp700I GAANNNNTTC 1 cut(s) 746
AspLEI GCGC 1 cut(s) 1172
AspS9I GGNCC 2 cut(s) 170, 867
AsuHPI GGTGA 2 cut(s) 217, 583
BaeGI GKGCMC 1 cut(s) 1059
BamHI GGATCC 1 cut(s) 154
BanI GGYRCC 3 cut(s) 700, 836, 994
BanII GRGCYC 2 cut(s) 69, 81
Bbv12I GWGCWC 2 cut(s) 69, 1059
BbvI GCAGC 2 cut(s) 187, 836
BccI CCATC 6 cut(s) 59, 399, 545, 758, 994, 1011
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 4 cut(s) 168, 417, 641, 991
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 941
BfaI CTAG 3 cut(s) 213, 300, 1014
BfmI CTRYAG 2 cut(s) 612, 1072
BfoI RGCGCY 1 cut(s) 1173
BglI GCCNNNNNGGC 1 cut(s) 675
BisI GCNGC 2 cut(s) 201, 825
BlsI GCNGC 2 cut(s) 202, 826
Bme1390I CCNGG 4 cut(s) 168, 417, 641, 991
BmgT120I GGNCC 2 cut(s) 170, 867
BmiI GGNNCC 9 cut(s) 78, 156, 386, 410, 702, 838, 996, 1085, 1117
BmrFI CCNGG 4 cut(s) 168, 417, 641, 991
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 8 cut(s) 127, 293, 349, 528, 653, 687, 811, 1012
BmuI ACTGGG 1 cut(s) 40
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BsaBI GATNNNNATC 1 cut(s) 783
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 941
BsaJI CCNNGG 7 cut(s) 150, 415, 639, 696, 863, 990, 998
BsaWI WCCGGW 1 cut(s) 1206
BsaXI ACNNNNNCTCC 2 cut(s) 712, 742
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 665, 697
Bse118I RCCGGY 2 cut(s) 791, 1206
Bse1I ACTGG 6 cut(s) 46, 132, 366, 386, 716, 932
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 4 cut(s) 168, 417, 641, 991
BseDI CCNNGG 7 cut(s) 150, 415, 639, 696, 863, 990, 998
BseGI GGATG 5 cut(s) 51, 128, 632, 644, 986
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 3 cut(s) 91, 665, 697
BseMII CTCAG 1 cut(s) 870
BseNI ACTGG 6 cut(s) 46, 132, 366, 386, 716, 932
BseRI GAGGAG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 1059
BseXI GCAGC 2 cut(s) 187, 836
BshFI GGCC 4 cut(s) 171, 669, 869, 1094
BshNI GGYRCC 3 cut(s) 700, 836, 994
BshTI ACCGGT 1 cut(s) 1206
BsiHKAI GWGCWC 2 cut(s) 69, 1059
BsiSI CCGG 2 cut(s) 792, 1207
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 3 cut(s) 91, 665, 697
BsmAI GTCTC 1 cut(s) 941
BsmFI GGGAC 1 cut(s) 331
BsnI GGCC 4 cut(s) 171, 669, 869, 1094
Bso31I GGTCTC 1 cut(s) 941
Bsp1286I GDGCHC 3 cut(s) 69, 81, 1059
Bsp143I GATC 5 cut(s) 88, 154, 360, 778, 919
Bsp19I CCATGG 2 cut(s) 696, 863
BspACI CCGC 7 cut(s) 55, 406, 497, 691, 734, 899, 1154
BspANI GGCC 4 cut(s) 171, 669, 869, 1094
BspCNI CTCAG 1 cut(s) 871
BspHI TCATGA 1 cut(s) 1180
BspLI GGNNCC 9 cut(s) 78, 156, 386, 410, 702, 838, 996, 1085, 1117
BspMAI CTGCAG 1 cut(s) 616
BspPI GGATC 3 cut(s) 149, 162, 773
BspT107I GGYRCC 3 cut(s) 700, 836, 994
BspTNI GGTCTC 1 cut(s) 941
BsrBI CCGCTC 1 cut(s) 57
BsrFI RCCGGY 2 cut(s) 791, 1206
BsrI ACTGG 6 cut(s) 46, 132, 366, 386, 716, 932
BssAI RCCGGY 2 cut(s) 791, 1206
BssECI CCNNGG 7 cut(s) 150, 415, 639, 696, 863, 990, 998
BssMI GATC 5 cut(s) 88, 154, 360, 778, 919
BssNI GRCGYC 1 cut(s) 113
BssT1I CCWWGG 4 cut(s) 150, 696, 863, 998
Bst2UI CCWGG 4 cut(s) 168, 417, 641, 991
Bst4CI ACNGT 3 cut(s) 818, 1028, 1088
Bst6I CTCTTC 2 cut(s) 15, 401
BstACI GRCGYC 1 cut(s) 113
BstC8I GCNNGC 5 cut(s) 36, 185, 198, 793, 1104
BstDEI CTNAG 3 cut(s) 602, 832, 879
BstDSI CCRYGG 2 cut(s) 696, 863
BstF5I GGATG 5 cut(s) 51, 128, 632, 644, 986
BstH2I RGCGCY 1 cut(s) 1173
BstHHI GCGC 1 cut(s) 1172
BstKTI GATC 5 cut(s) 91, 157, 363, 781, 922
BstMAI GTCTC 1 cut(s) 941
BstMBI GATC 5 cut(s) 88, 154, 360, 778, 919
BstMWI GCNNNNNNNGC 5 cut(s) 146, 193, 675, 801, 896
BstNI CCWGG 4 cut(s) 168, 417, 641, 991
BstNSI RCATGY 2 cut(s) 200, 340
BstSCI CCNGG 4 cut(s) 166, 415, 639, 989
BstSFI CTRYAG 2 cut(s) 612, 1072
BstSLI GKGCMC 1 cut(s) 1059
BstV1I GCAGC 2 cut(s) 187, 836
BstX2I RGATCY 1 cut(s) 154
BstXI CCANNNNNNTGG 3 cut(s) 723, 744, 1140
BstYI RGATCY 1 cut(s) 154
BsuRI GGCC 4 cut(s) 171, 669, 869, 1094
BtgI CCRYGG 2 cut(s) 696, 863
BtsCI GGATG 5 cut(s) 51, 128, 632, 644, 986
BtsIMutI CAGTG 4 cut(s) 139, 379, 823, 876
Cac8I GCNNGC 5 cut(s) 36, 185, 198, 793, 1104
CciI TCATGA 1 cut(s) 1180
CfoI GCGC 1 cut(s) 1172
Cfr10I RCCGGY 2 cut(s) 791, 1206
Cfr13I GGNCC 2 cut(s) 170, 867
CseI GACGC 2 cut(s) 102, 411
Csp6I GTAC 3 cut(s) 285, 490, 1011
CspAI ACCGGT 1 cut(s) 1206
CviQI GTAC 3 cut(s) 285, 490, 1011
DdeI CTNAG 3 cut(s) 602, 832, 879
DpnI GATC 5 cut(s) 90, 156, 362, 780, 921
DpnII GATC 5 cut(s) 88, 154, 360, 778, 919
EaeI YGGCCR 1 cut(s) 1092
Eam1104I CTCTTC 2 cut(s) 15, 401
EarI CTCTTC 2 cut(s) 15, 401
EciI GGCGGA 2 cut(s) 680, 1143
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 4 cut(s) 150, 696, 863, 998
Eco24I GRGCYC 2 cut(s) 69, 81
Eco31I GGTCTC 1 cut(s) 941
Eco47III AGCGCT 1 cut(s) 1171
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 257, 382
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 4 cut(s) 166, 415, 639, 989
EcoT14I CCWWGG 4 cut(s) 150, 696, 863, 998
EcoT22I ATGCAT 1 cut(s) 342
EcoT38I GRGCYC 2 cut(s) 69, 81
ErhI CCWWGG 4 cut(s) 150, 696, 863, 998
FaqI GGGAC 1 cut(s) 331
FauI CCCGC 1 cut(s) 741
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 201, 825
FokI GGATG 5 cut(s) 38, 115, 619, 631, 973
FriOI GRGCYC 2 cut(s) 69, 81
Fsp4HI GCNGC 2 cut(s) 201, 825
FspBI CTAG 3 cut(s) 213, 300, 1014
GlaI GCGC 1 cut(s) 1171
GluI GCNGC 2 cut(s) 201, 825
HaeII RGCGCY 1 cut(s) 1173
HaeIII GGCC 4 cut(s) 171, 669, 869, 1094
HapII CCGG 2 cut(s) 792, 1207
HgaI GACGC 2 cut(s) 102, 411
HhaI GCGC 1 cut(s) 1172
Hin1I GRCGYC 1 cut(s) 113
Hin6I GCGC 1 cut(s) 1170
HinP1I GCGC 1 cut(s) 1170
HinfI GANTC 6 cut(s) 329, 608, 727, 953, 1039, 1145
HpaII CCGG 2 cut(s) 792, 1207
HphI GGTGA 2 cut(s) 217, 583
Hpy166II GTNNAC 1 cut(s) 1057
Hpy188I TCNGA 6 cut(s) 106, 276, 328, 351, 401, 763
Hpy188III TCNNGA 5 cut(s) 300, 573, 776, 1162, 1181
Hpy8I GTNNAC 1 cut(s) 1057
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 4 cut(s) 168, 183, 850, 1073
HpyCH4III ACNGT 3 cut(s) 818, 1028, 1088
HpyCH4IV ACGT 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 193, 675, 801, 896
HpyF3I CTNAG 3 cut(s) 602, 832, 879
HpySE526I ACGT 1 cut(s) 29
Hsp92I GRCGYC 1 cut(s) 113
HspAI GCGC 1 cut(s) 1170
KroI GCCGGC 1 cut(s) 791
KroNI GCCGGC 1 cut(s) 793
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 5 cut(s) 88, 154, 360, 778, 919
LmnI GCTCC 6 cut(s) 72, 76, 390, 432, 557, 1115
Lsp1109I GCAGC 2 cut(s) 187, 836
LweI GCATC 8 cut(s) 127, 293, 349, 528, 653, 687, 811, 1012
MaeI CTAG 3 cut(s) 213, 300, 1014
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 5 cut(s) 21, 223, 652, 718, 923
MalI GATC 5 cut(s) 90, 156, 362, 780, 921
MbiI CCGCTC 1 cut(s) 57
MboI GATC 5 cut(s) 88, 154, 360, 778, 919
MboII GAAGA 3 cut(s) 263, 388, 998
MfeI CAATTG 3 cut(s) 60, 855, 1046
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 3 cut(s) 69, 81, 1059
MlyI GAGTC 1 cut(s) 721
MmeI TCCRAC 1 cut(s) 1157
Mph1103I ATGCAT 1 cut(s) 342
MroNI GCCGGC 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 746
MseI TTAA 4 cut(s) 579, 620, 906, 978
MspI CCGG 2 cut(s) 792, 1207
MspR9I CCNGG 4 cut(s) 168, 417, 641, 991
MunI CAATTG 3 cut(s) 60, 855, 1046
MvaI CCWGG 4 cut(s) 168, 417, 641, 991
MwoI GCNNNNNNNGC 5 cut(s) 146, 193, 675, 801, 896
NaeI GCCGGC 1 cut(s) 793
NcoI CCATGG 2 cut(s) 696, 863
NdeII GATC 5 cut(s) 88, 154, 360, 778, 919
NgoMIV GCCGGC 1 cut(s) 791
NlaIV GGNNCC 9 cut(s) 78, 156, 386, 410, 702, 838, 996, 1085, 1117
NmuCI GTSAC 2 cut(s) 223, 718
NsiI ATGCAT 1 cut(s) 342
NspI RCATGY 2 cut(s) 200, 340
PaeI GCATGC 1 cut(s) 200
PagI TCATGA 1 cut(s) 1180
PdiI GCCGGC 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 746
PfeI GAWTC 5 cut(s) 329, 608, 953, 1039, 1145
PinAI ACCGGT 1 cut(s) 1206
PkrI GCNGC 2 cut(s) 202, 826
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
Psp124BI GAGCTC 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 29
Psp6I CCWGG 4 cut(s) 166, 415, 639, 989
PspGI CCWGG 4 cut(s) 166, 415, 639, 989
PspN4I GGNNCC 9 cut(s) 78, 156, 386, 410, 702, 838, 996, 1085, 1117
PspPI GGNCC 2 cut(s) 170, 867
PstI CTGCAG 1 cut(s) 616
PsuI RGATCY 1 cut(s) 154
RsaI GTAC 3 cut(s) 286, 491, 1012
RsaNI GTAC 3 cut(s) 285, 490, 1011
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 4 cut(s) 579, 620, 906, 978
SatI GCNGC 2 cut(s) 201, 825
Sau3AI GATC 5 cut(s) 88, 154, 360, 778, 919
Sau96I GGNCC 2 cut(s) 170, 867
SchI GAGTC 1 cut(s) 721
ScrFI CCNGG 4 cut(s) 168, 417, 641, 991
SduI GDGCHC 3 cut(s) 69, 81, 1059
SfaNI GCATC 8 cut(s) 127, 293, 349, 528, 653, 687, 811, 1012
SfcI CTRYAG 2 cut(s) 612, 1072
SphI GCATGC 1 cut(s) 200
SsiI CCGC 7 cut(s) 55, 406, 497, 691, 734, 899, 1154
SspI AATATT 1 cut(s) 624
SspMI CTAG 3 cut(s) 213, 300, 1014
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 166, 415, 639, 989
StyI CCWWGG 4 cut(s) 150, 696, 863, 998
TaaI ACNGT 3 cut(s) 818, 1028, 1088
TaiI ACGT 1 cut(s) 32
TaqI TCGA 2 cut(s) 465, 1037
TaqII GACCGA 1 cut(s) 965
TfiI GAWTC 5 cut(s) 329, 608, 953, 1039, 1145
Tru1I TTAA 4 cut(s) 579, 620, 906, 978
Tru9I TTAA 4 cut(s) 579, 620, 906, 978
TscAI CASTG 4 cut(s) 139, 386, 823, 883
TseFI GTSAC 2 cut(s) 223, 718
TseI GCWGC 2 cut(s) 200, 824
Tsp45I GTSAC 2 cut(s) 223, 718
TspDTI ATGAA 3 cut(s) 17, 165, 360
TspGWI ACGGA 1 cut(s) 1214
TspRI CASTG 4 cut(s) 139, 386, 823, 883
VneI GTGCAC 1 cut(s) 1055
XapI RAATTY 3 cut(s) 252, 322, 757
XbaI TCTAGA 1 cut(s) 299
XceI RCATGY 2 cut(s) 200, 340
XmnI GAANNNNTTC 1 cut(s) 746
XspI CTAG 3 cut(s) 213, 300, 1014
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.