Rorug07G0312000
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000636
Physical Location & Seq
Reverse (-)
31275973 .. 31283105
7133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0312000.1

Sequence Viewer

Length: 2853 bp
ATGGTGAAGTCGTACTTGCGATACGAGCAGGGTGCCGCCTTCGGCGTGATAGTTTCTGGCAATTCAAACATCGCCTACGACAGTTCCGGGAAGCACATTTTTGCCCCGGGCCTGGAAAAGGTGGGCGTGTGGAACGTGCGACAAGGCATTTGCATCAAAACCTTAACTCCTTGTGCTGCTGATGGATATTCGCCGCCAGTCACCTCCATCGCGGTCTCGCCTTCTTCTTCTTCTTCTCTGATAGCCACTGGGTATGGAGATGGGAGCATTAGAATTTGGGACTCTCAGAATAAAATTTGTGAGACCACATTCTACGGTCATACGGGGGCCGTTAGTGTCTTACGGTACAACAAGATTGGAGATAGGCTGGTTTCTGGTGGTCAAGATAAGGACATTATATTGTGGGATGTGGAGAGGGGCGAGGGCATCTATCGCCTCCGCGGGCATGGGAATCAGGTCACTGACCTCGTCTTCTTGGACCGTTGTAAGAAACTCGTTAGTTCCTCAAAAGACAAATTTTTGAAGGTCTGGGATCTTGAAACCCCCCATTGCATGCAGACAATAAGTGACCACCATAGTGAAATTTGGTCCATAGATATCGATCCGGAAGAAAGATATTTGGTCTCTGGCTCTGCTGACTTGGAACTTAGGTTTTACACAATTAAGCATGACCTTGAAGCTTGCCAATCCACTTCTAATGCGAATGCAAATGGAACAGACTCTGGAGATCCATCCCCTCATAATAAATGGCAAGTTTTGAAGCTGTTTGGTGAATATCAGCGAAAAAGCAAGAACAGAGTTGCAACTGTGAGATTCAACAAGTCTGGAAATTTGCTGGCATGTCAAGAGGCAGGAAAGGCGGTACATATATTCAACGTACTGGATGAGGCTGAATCTACCCGTAAAGCAAACCGCAGGATTCGCCGGATCAAAAAGAAAAAGTCTGCCCAAGGGGAAGCCGAGGTGATGAAGGATGATGGAGCTGGAAAAGAGGGAAAGAGGCACGTGGTCATGGTCTCTGATGTTTTTCAACTTTTCCAAACTATTCGACCTGGTAAAAAGATATGTTCCATCTCTTTTTGTCCAAACCCTCCCAAGACTTCCCTAGCCACAATAGCTTTGTCTCTGAGCGATAATCTGTGCGTGTTTTATTCTCTTGAGAGCGAAGCAACAAAACAAACACGCTCTATAGACCTCCACGGCCACCGATCATCTGTCACAAGTGTAGCGCTTAGCTCAGACAACACAGTTTTGATGTCAACCAGTCATAATGCAGCAAAGATCTGGAATCCAACCAATGGTTACTGCCTTGGAACTATTGACACAGGATTCGCCATGTGTGCTTTTATCCTTCCTTCTTGCAATAACAAGTTTGCACTTGTTGGAACCAAGGGTGGAACAATAGAAATTATTGACGTTGGGAGTGCAACTGTCACTGAAGTAGTAGAAGCTCATGGTGGCTCTGTTCAGTCTATTGCAAGAATTCCCAATGAAAATGGTTTTGTCACAGGAAGCTCTGATGGTTATGTTAAGTTCTGGGAATATGGAGTCAAAGAAGATTCTAAGCGCCTGCTGGTGTCAAATGTAAGAATGAAGGAGATGGATACTGATGTTCGTGTGGTTTCTATCAGTCCTGATGCCAAATATCTTTTGGTGGCGTTTGATACCATAGTAAAGGTATTATATATGGATTCACTCAAACTTTTCGTCACCTTATATGGTCACAAGCTGCCAGTGCTATGTCTCGATGTCTCATCCGATGGAGAGTTGATAGTGACTGGATCTCAAGACAAAAATGTGAAGATTTGGGGGCTAGATTTTGGTGACTGCCACAAATCAATGTTTGATCATCAGAGCAGCGTTCAGCAAGTACAATTTGTGCCAAATACCCATTACTTTGTTAGTGTTGGGGATGATAAGCAAGTAAACTACTGGGATGCTGATAAATTTGAGTTGCTTTTAACTATGAAGGGACACCATGAGGGTGTTTGTTGCCTCGCTATTAGTAACCGTGGTGATTTCTTTGTCACGGGATCTCATGATCGCTCAATTCGCCGCTGGGATCGTACTGATGAGCCATTTTTCCTTGAGGAAGAGAGGGAAAAACGGGAGTTGGAAAAGATGTACGAGTCTGACTTCAAGGATGAATATGAGAACAATTTTGCACTCAAGGAAAAGATCCCTGCAGAGGGAGCTGTGGCTTTTGCTGGGAAGAAAACTAAAGAAACAATTTCTTTGACCGACTCAATTGTTGCTAAACTAGAAATAGCATGGGAAGAATCAAAGCAACGAGCCAATTATGAGGCGGAAAAAGTCAGTGGAAAGCTGGTTGCTGAGTTTCAACCAAATCCTTTTATGCTAGGGCTGTCTCCCCCTGATTATTTTCTGCATGAACTTTTGAAGGTTAAGACAAGCGATTTGGAGTCGATGCTATTGGGGCTACCCTTCTCGGATGCCTTGAAGATTTTGTCTTTCTGCAAGGATGTTGGTCTACGTCCTGATGAGGTTGAATTCATTACCAGGGTGGTTACATTGCTGCTGCAGATAAATTGTAATCAATTGATTTCCACTCCAGCTGTAAAACCAATATTGACCGGTTTAAAAGAGGTTCTTTATCCCAACTTAAAGGCAAAGAAGGATATCATTGGTTACAACCTTGCAGCAATGACTCATATCAAGCAATTGCATGATTCAAGATCAAATGCACCACTTCCAGATGCAATCTCCAAGCGTGGTGTAGGTGCAAGTTGGTATGGAAAGACAGAAGAAGGTAAATGCAAAAAAGTTCGAACATGTGAACCCACGCCTGGCATGCATCGACACAGTTGGTTACAGCCCTGCAGCAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

950

Amino Acids

105.61

Weight (kDa)

7.52

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 19 - 188 6.3e-12 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 23 - 221 3.1e-27 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 24 - 223 8.2e-33 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 29 - 224 1.2e-21 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 32 - 221 5e-67 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 32 - 138 1.4e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 40 - 211 3.7e-13 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 41 - 177 1.2e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR75_1st PF23869 59 - 138 9.5e-07 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR3_2nd PF25172 63 - 178 1.5e-14 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 66 - 266 6.9e-14 CAF1B/HIR1 beta-propeller domain
WDR55 PF24796 67 - 231 1.7e-14 WDR55
Beta-prop_EML_2 PF23414 70 - 224 1.9e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_IFT140_1st PF23383 78 - 138 3.4e-06 IFT140 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 79 - 179 1e-08 MABP1/WDR62 second WD40 domain
Beta-prop_SCAP PF24017 79 - 181 1.3e-11 SCAP Beta-propeller
EIF3I PF24805 105 - 189 1.8e-10 EIF3I
WD40_Gbeta PF25391 106 - 228 9.5e-13 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 120 - 234 1.4e-20 Prp19 WD40 domain
Beta-prop_WDR41 PF25178 121 - 216 5.5e-09 WDR41
Beta-prop_TEP1_2nd PF25047 122 - 197 2.4e-07 TEP-1 second beta-propeller
EIF3I PF24805 146 - 225 2.3e-08 EIF3I
Beta-prop_WDR3_2nd PF25172 174 - 289 1.1e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 354 - 553 1.9e-14 WDR5 beta-propeller domain
WD40_Gbeta PF25391 372 - 603 3e-16 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 376 - 612 2.8e-24 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 406 - 693 1.1e-109 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 448 - 636 4.5e-13 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 455 - 565 1.4e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 455 - 687 2e-22 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 455 - 646 4.3e-23 Prp19 WD40 domain
WDR55 PF24796 463 - 688 7.6e-15 WDR55
Beta-prop_EML_2 PF23414 491 - 627 7.1e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 498 - 689 2.8e-15 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 531 - 688 9.8e-27 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 531 - 688 6.1e-10 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 534 - 694 1e-17 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 535 - 690 1.1e-29 WDR5 beta-propeller domain
Beta-prop_IFT122_1st PF23381 539 - 638 1.5e-06 IFT122 first beta-propeller
EIF3I PF24805 552 - 632 6.9e-08 EIF3I
WD40_Gbeta PF25391 552 - 689 3.5e-11 G protein beta WD-40 repeat protein
WD40 PF00400 569 - 603 1e-08 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 570 - 648 4.8e-06 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 579 - 687 2.3e-09 MABP1/WDR62 first WD40 domain
EIF3I PF24805 616 - 689 2.2e-06 EIF3I
Beta-prop_THOC3 PF25174 617 - 688 2.6e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 630 - 693 5.9e-08 Prp19 WD40 domain
WD40 PF00400 651 - 688 1.3e-08 WD domain, G-beta repeat
Utp12 PF04003 794 - 894 8.5e-16 Dip2/Utp12 Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000636)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36290 FvH4_5g27770 FvH4_5g29860 FvH4_5g37342 FvH4_5g37877 FvH4_5g37880 FvH4_5g37900 FvH4_6g40020
rosa_chinensis RchiOBHm_Chr7g0227221 RchiOBHm_Chr7g0239011
rosa_multiflora Rmu_co8202712.1_g000001 Rmu_sc0000477.1_g000006 Rmu_sc0001047.1_g000050 Rmu_sc0001371.1_g000021 Rmu_sc0002449.1_g000018 Rmu_sc0002988.1_g000003 Rmu_sc0003089.1_g000031 Rmu_sc0004062.1_g000013 Rmu_sc0004132.1_g000001 Rmu_sc0004277.1_g000045 Rmu_sc0004567.1_g000017 Rmu_sc0005327.1_g000024 Rmu_sc0005651.1_g000005 Rmu_sc0006669.1_g000014 Rmu_sc0018004.1_g000002 Rmu_sc0018978.1_g000001 Rmu_sc0021209.1_g000001 Rmu_sc0034369.1_g000001 Rmu_ssc0000398.1_g000019
rosa_roxburghii Rroxscaffold_1G00004410 Rroxscaffold_3G00220090 Rroxscaffold_3G00221380 Rroxscaffold_3G00222090 Rroxscaffold_3G00223100 Rroxscaffold_6G00400160 Rroxscaffold_7G00204490 Rroxscaffold_7G00204500
rosa_rugosa Rorug02G0147900 Rorug03G0117000 Rorug03G0194000 Rorug04G0112500 Rorug05G0218600 Rorug05G0218700 Rorug05G0218800 Rorug05G0218900 Rorug05G0219000 Rorug05G0219000 Rorug05G0219000 Rorug05G0219100 Rorug05G0220000 Rorug05G0574900 Rorug07G0311900 Rorug07G0312000 Rorug07G0324500 Rorug07G0324600
rosa_samantha Rh3BG280100 Rh4BG071800 Rh5BG306100 Rh5BG308200 Rh5BG313000 Rh5BG335200 Rh5BG532100 Rh5BG532200 Rh5CG556100 Rh5DG315400 Rh5DG318300 Rh5DG318400 Rh5DG347500 Rh6CG116800 Rh7BG420800 Rh7BG437600 Rh7BG452200 Rh7CG484800 Rh7CG496900 Rh7DG453200 Rh7DG464300
rosa_wichuraiana Rw7G039600 Rw7G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 32
AccB7I CCANNNNNTGG 1 cut(s) 1298
AccI GTMKAC 1 cut(s) 2491
AccII CGCG 2 cut(s) 212, 441
AccIII TCCGGA 1 cut(s) 604
AciI CCGC 9 cut(s) 36, 194, 212, 439, 441, 860, 913, 2056, 2306
AclWI GGATC 8 cut(s) 540, 596, 722, 935, 1789, 2041, 2070, 2173
AcoI YGGCCR 1 cut(s) 1201
AcsI RAATTY 8 cut(s) 273, 294, 515, 582, 829, 1482, 1946, 2510
AcuI CTGAAG 1 cut(s) 1458
AcvI CACGTG 1 cut(s) 1006
AfaI GTAC 7 cut(s) 14, 347, 864, 879, 1872, 2068, 2126
AfeI AGCGCT 1 cut(s) 1230
AfiI CCNNNNNNNGG 6 cut(s) 112, 118, 1298, 2188, 2189, 2807
AflIII ACRYGT 1 cut(s) 2792
AgeI ACCGGT 1 cut(s) 2594
AjnI CCWGG 4 cut(s) 111, 1051, 2519, 2806
AleI CACNNNNGTG 1 cut(s) 576
Alw26I GTCTC 8 cut(s) 220, 296, 628, 1021, 1128, 1748, 1756, 2373
AlwI GGATC 8 cut(s) 540, 596, 722, 935, 1789, 2041, 2070, 2173
AlwNI CAGNNNCTG 1 cut(s) 722
Ama87I CYCGRG 1 cut(s) 106
Aor13HI TCCGGA 1 cut(s) 604
Aor51HI AGCGCT 1 cut(s) 1230
AoxI GGCC 3 cut(s) 109, 327, 1201
ApeKI GCWGC 8 cut(s) 176, 1274, 1729, 1857, 2536, 2539, 2660, 2841
ApoI RAATTY 8 cut(s) 273, 294, 515, 582, 829, 1482, 1946, 2510
ArsI GACNNNNNNTTYG 8 cut(s) 132, 164, 1313, 1345, 1692, 1724, 2401, 2433
AsiGI ACCGGT 1 cut(s) 2594
AspLEI GCGC 2 cut(s) 1231, 1569
AspS9I GGNCC 4 cut(s) 109, 327, 478, 588
AsuC2I CCSGG 3 cut(s) 88, 107, 108
AsuHPI GGTGA 7 cut(s) 16, 193, 782, 976, 1702, 1835, 2027
AsuII TTCGAA 1 cut(s) 2788
AvaI CYCGRG 1 cut(s) 106
AvaII GGWCC 2 cut(s) 478, 588
BaeI ACNNNNGTAYC 4 cut(s) 1596, 1629, 1656, 1689
BanI GGYRCC 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 1006
BbsI GAAGAC 1 cut(s) 463
BbvI GCAGC 7 cut(s) 163, 1286, 1716, 1869, 2523, 2526, 2672
BccI CCATC 9 cut(s) 176, 215, 254, 739, 971, 1079, 1514, 1594, 1754
BceAI ACGGC 2 cut(s) 314, 1216
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 4 cut(s) 113, 1053, 2521, 2808
BciVI GTATCC 1 cut(s) 1597
BclI TGATCA 1 cut(s) 1846
BcnI CCSGG 3 cut(s) 88, 107, 108
BcoDI GTCTC 8 cut(s) 220, 296, 628, 1021, 1128, 1748, 1756, 2373
BfaI CTAG 4 cut(s) 1106, 1814, 2261, 2360
BfmI CTRYAG 4 cut(s) 1188, 2184, 2540, 2839
BfoI RGCGCY 2 cut(s) 1232, 1570
BfuI GTATCC 1 cut(s) 1597
BglII AGATCT 1 cut(s) 1281
BlpI GCTNAGC 1 cut(s) 1232
Bme1390I CCNGG 7 cut(s) 88, 107, 108, 113, 1053, 2521, 2808
Bme18I GGWCC 2 cut(s) 478, 588
BmeT110I CYCGRG 1 cut(s) 106
BmgT120I GGNCC 4 cut(s) 109, 327, 478, 588
BmiI GGNNCC 3 cut(s) 34, 328, 1387
BmrFI CCNGG 7 cut(s) 88, 107, 108, 113, 1053, 2521, 2808
BmrI ACTGGG 2 cut(s) 258, 1942
BmsI GCATC 8 cut(s) 162, 435, 1627, 1927, 2418, 2443, 2707, 2824
BmuI ACTGGG 2 cut(s) 258, 1942
BpiI GAAGAC 1 cut(s) 463
BpmI CTGGAG 2 cut(s) 744, 2556
Bpu1102I GCTNAGC 1 cut(s) 1232
Bpu14I TTCGAA 1 cut(s) 2788
BpuEI CTTGAG 4 cut(s) 1178, 1770, 2108, 2153
BpuMI CCSGG 3 cut(s) 88, 107, 108
Bsa29I ATCGAT 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 1006
BsaBI GATNNNNATC 1 cut(s) 600
BsaI GGTCTC 4 cut(s) 220, 296, 628, 1021
BsaWI WCCGGW 2 cut(s) 604, 2594
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 6 cut(s) 112, 118, 1298, 2188, 2189, 2807
Bse118I RCCGGY 1 cut(s) 2594
Bse1I ACTGG 7 cut(s) 197, 253, 885, 1263, 1733, 1783, 1937
Bse3DI GCAATG 3 cut(s) 547, 2531, 2670
Bse8I GATNNNNATC 1 cut(s) 600
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 4 cut(s) 113, 1053, 2521, 2808
BseCI ATCGAT 1 cut(s) 600
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 6 cut(s) 112, 118, 1298, 2188, 2189, 2807
BseMI GCAATG 3 cut(s) 547, 2531, 2670
BseMII CTCAG 4 cut(s) 299, 1118, 1251, 2325
BseNI ACTGG 7 cut(s) 197, 253, 885, 1263, 1733, 1783, 1937
BseXI GCAGC 7 cut(s) 163, 1286, 1716, 1869, 2523, 2526, 2672
BseYI CCCAGC 2 cut(s) 2058, 2207
Bsh1236I CGCG 2 cut(s) 212, 441
BshFI GGCC 3 cut(s) 111, 329, 1203
BshNI GGYRCC 1 cut(s) 32
BshTI ACCGGT 1 cut(s) 2594
BshVI ATCGAT 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 5 cut(s) 87, 107, 605, 925, 2595
BslFI GGGAC 2 cut(s) 293, 1986
BslI CCNNNNNNNGG 6 cut(s) 112, 118, 1298, 2188, 2189, 2807
BsmAI GTCTC 8 cut(s) 220, 296, 628, 1021, 1128, 1748, 1756, 2373
BsmFI GGGAC 2 cut(s) 293, 1986
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 3 cut(s) 111, 329, 1203
Bso31I GGTCTC 4 cut(s) 220, 296, 628, 1021
BsoBI CYCGRG 1 cut(s) 106
Bsp119I TTCGAA 1 cut(s) 2788
Bsp13I TCCGGA 1 cut(s) 604
Bsp1720I GCTNAGC 1 cut(s) 1232
BspACI CCGC 9 cut(s) 36, 194, 212, 439, 441, 860, 913, 2056, 2306
BspANI GGCC 3 cut(s) 111, 329, 1203
BspCNI CTCAG 4 cut(s) 298, 1119, 1250, 2326
BspDI ATCGAT 1 cut(s) 600
BspEI TCCGGA 1 cut(s) 604
BspFNI CGCG 2 cut(s) 212, 441
BspHI TCATGA 1 cut(s) 2038
BspLI GGNNCC 3 cut(s) 34, 328, 1387
BspMAI CTGCAG 3 cut(s) 2188, 2544, 2843
BspPI GGATC 8 cut(s) 540, 596, 722, 935, 1789, 2041, 2070, 2173
BspT104I TTCGAA 1 cut(s) 2788
BspT107I GGYRCC 1 cut(s) 32
BspTNI GGTCTC 4 cut(s) 220, 296, 628, 1021
BsrDI GCAATG 3 cut(s) 547, 2531, 2670
BsrFI RCCGGY 1 cut(s) 2594
BsrI ACTGG 7 cut(s) 197, 253, 885, 1263, 1733, 1783, 1937
BssAI RCCGGY 1 cut(s) 2594
BssT1I CCWWGG 3 cut(s) 949, 1309, 1389
Bst2UI CCWGG 4 cut(s) 113, 1053, 2521, 2808
Bst4CI ACNGT 9 cut(s) 83, 317, 345, 482, 808, 1249, 1432, 2012, 2825
Bst6I CTCTTC 1 cut(s) 2088
BstBAI YACGTR 1 cut(s) 1006
BstBI TTCGAA 1 cut(s) 2788
BstC8I GCNNGC 6 cut(s) 443, 554, 682, 837, 1571, 2813
BstDEI CTNAG 7 cut(s) 285, 647, 1127, 1232, 1237, 1563, 2334
BstDSI CCRYGG 3 cut(s) 439, 1198, 2011
BstENI CCTNNNNNAGG 1 cut(s) 116
BstFNI CGCG 2 cut(s) 212, 441
BstH2I RGCGCY 2 cut(s) 1232, 1570
BstHHI GCGC 2 cut(s) 1231, 1569
BstMAI GTCTC 8 cut(s) 220, 296, 628, 1021, 1128, 1748, 1756, 2373
BstNI CCWGG 4 cut(s) 113, 1053, 2521, 2808
BstNSI RCATGY 4 cut(s) 556, 843, 2796, 2815
BstSCI CCNGG 7 cut(s) 86, 105, 106, 111, 1051, 2519, 2806
BstSFI CTRYAG 4 cut(s) 1188, 2184, 2540, 2839
BstUI CGCG 2 cut(s) 212, 441
BstV1I GCAGC 7 cut(s) 163, 1286, 1716, 1869, 2523, 2526, 2672
BstV2I GAAGAC 1 cut(s) 463
BstX2I RGATCY 6 cut(s) 532, 727, 1281, 1781, 2033, 2178
BstYI RGATCY 6 cut(s) 532, 727, 1281, 1781, 2033, 2178
Bsu15I ATCGAT 1 cut(s) 600
BsuI GTATCC 1 cut(s) 1597
BsuRI GGCC 3 cut(s) 111, 329, 1203
BsuTUI ATCGAT 1 cut(s) 600
BtgI CCRYGG 3 cut(s) 439, 1198, 2011
BtgZI GCGATG 2 cut(s) 55, 193
BtsIMutI CAGTG 5 cut(s) 246, 459, 1434, 1740, 2323
Cac8I GCNNGC 6 cut(s) 443, 554, 682, 837, 1571, 2813
CaiI CAGNNNCTG 1 cut(s) 722
CciI TCATGA 1 cut(s) 2038
CfoI GCGC 2 cut(s) 1231, 1569
Cfr10I RCCGGY 1 cut(s) 2594
Cfr13I GGNCC 4 cut(s) 109, 327, 478, 588
Cfr42I CCGCGG 1 cut(s) 442
Cfr9I CCCGGG 1 cut(s) 106
ClaI ATCGAT 1 cut(s) 600
CsiI ACCWGGT 1 cut(s) 1051
Csp6I GTAC 7 cut(s) 13, 346, 863, 878, 1871, 2067, 2125
CspAI ACCGGT 1 cut(s) 2594
CviQI GTAC 7 cut(s) 13, 346, 863, 878, 1871, 2067, 2125
DdeI CTNAG 7 cut(s) 285, 647, 1127, 1232, 1237, 1563, 2334
DraI TTTAAA 1 cut(s) 2601
EaeI YGGCCR 1 cut(s) 1201
Eam1104I CTCTTC 1 cut(s) 2088
EarI CTCTTC 1 cut(s) 2088
EciI GGCGGA 1 cut(s) 2321
Eco130I CCWWGG 3 cut(s) 949, 1309, 1389
Eco31I GGTCTC 4 cut(s) 220, 296, 628, 1021
Eco32I GATATC 2 cut(s) 598, 2641
Eco47I GGWCC 2 cut(s) 478, 588
Eco47III AGCGCT 1 cut(s) 1230
Eco57I CTGAAG 1 cut(s) 1458
Eco72I CACGTG 1 cut(s) 1006
Eco88I CYCGRG 1 cut(s) 106
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoRI GAATTC 2 cut(s) 1482, 2510
EcoRII CCWGG 4 cut(s) 111, 1051, 2519, 2806
EcoRV GATATC 2 cut(s) 598, 2641
EcoT14I CCWWGG 3 cut(s) 949, 1309, 1389
EcoT22I ATGCAT 1 cut(s) 2817
ErhI CCWWGG 3 cut(s) 949, 1309, 1389
FalI AAGNNNNNCTT 3 cut(s) 31, 2595, 2627
FaqI GGGAC 2 cut(s) 293, 1986
FauI CCCGC 1 cut(s) 434
FbaI TGATCA 1 cut(s) 1846
FblI GTMKAC 1 cut(s) 2491
FspBI CTAG 4 cut(s) 1106, 1814, 2261, 2360
GlaI GCGC 2 cut(s) 1230, 1568
GsaI CCCAGC 2 cut(s) 2062, 2211
GsuI CTGGAG 2 cut(s) 744, 2556
HaeII RGCGCY 2 cut(s) 1232, 1570
HaeIII GGCC 3 cut(s) 111, 329, 1203
HapII CCGG 5 cut(s) 87, 107, 605, 925, 2595
HhaI GCGC 2 cut(s) 1231, 1569
Hin6I GCGC 2 cut(s) 1229, 1567
HinP1I GCGC 2 cut(s) 1229, 1567
HincII GTYRAC 1 cut(s) 1260
HindII GTYRAC 1 cut(s) 1260
HindIII AAGCTT 1 cut(s) 678
HpaII CCGG 5 cut(s) 87, 107, 605, 925, 2595
HphI GGTGA 7 cut(s) 16, 193, 782, 976, 1702, 1835, 2027
Hpy166II GTNNAC 4 cut(s) 1260, 1927, 2492, 2798
Hpy8I GTNNAC 4 cut(s) 1260, 1927, 2492, 2798
HpyCH4III ACNGT 9 cut(s) 83, 317, 345, 482, 808, 1249, 1432, 2012, 2825
HpyCH4IV ACGT 5 cut(s) 135, 876, 1005, 1416, 2494
HpyF3I CTNAG 7 cut(s) 285, 647, 1127, 1232, 1237, 1563, 2334
HpySE526I ACGT 5 cut(s) 135, 876, 1005, 1416, 2494
HspAI GCGC 2 cut(s) 1229, 1567
Kpn2I TCCGGA 1 cut(s) 604
Ksp22I TGATCA 1 cut(s) 1846
KspI CCGCGG 1 cut(s) 442
LmnI GCTCC 3 cut(s) 264, 980, 2192
Lsp1109I GCAGC 7 cut(s) 163, 1286, 1716, 1869, 2523, 2526, 2672
LweI GCATC 8 cut(s) 162, 435, 1627, 1927, 2418, 2443, 2707, 2824
MabI ACCWGGT 1 cut(s) 1051
MaeI CTAG 4 cut(s) 1106, 1814, 2261, 2360
MaeII ACGT 5 cut(s) 135, 876, 1005, 1416, 2494
MfeI CAATTG 3 cut(s) 2247, 2558, 2681
MflI RGATCY 6 cut(s) 532, 727, 1281, 1781, 2033, 2178
MlyI GAGTC 7 cut(s) 275, 713, 1557, 2138, 2237, 2432, 2662
MmeI TCCRAC 3 cut(s) 1316, 1363, 2094
Mph1103I ATGCAT 1 cut(s) 2817
MroI TCCGGA 1 cut(s) 604
MseI TTAA 7 cut(s) 164, 663, 1530, 1961, 2406, 2600, 2624
MslI CAYNNNNRTG 2 cut(s) 576, 1983
MspA1I CMGCKG 3 cut(s) 441, 2058, 2576
MspI CCGG 5 cut(s) 87, 107, 605, 925, 2595
MspR9I CCNGG 7 cut(s) 88, 107, 108, 113, 1053, 2521, 2808
MunI CAATTG 3 cut(s) 2247, 2558, 2681
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 4 cut(s) 113, 1053, 2521, 2808
MvnI CGCG 2 cut(s) 212, 441
NciI CCSGG 3 cut(s) 88, 107, 108
NlaIV GGNNCC 3 cut(s) 34, 328, 1387
NmeAIII GCCGAG 1 cut(s) 985
NsiI ATGCAT 1 cut(s) 2817
NspI RCATGY 4 cut(s) 556, 843, 2796, 2815
NspV TTCGAA 1 cut(s) 2788
OliI CACNNNNGTG 1 cut(s) 576
PaeI GCATGC 2 cut(s) 556, 2815
PagI TCATGA 1 cut(s) 2038
PciI ACATGT 1 cut(s) 2792
PctI GAATGC 1 cut(s) 709
PflFI GACNNNGTC 1 cut(s) 467
PflMI CCANNNNNTGG 1 cut(s) 1298
PfoI TCCNGGA 1 cut(s) 86
PinAI ACCGGT 1 cut(s) 2594
PleI GAGTC 7 cut(s) 275, 713, 1556, 2137, 2237, 2431, 2662
PmaCI CACGTG 1 cut(s) 1006
PmlI CACGTG 1 cut(s) 1006
PpsI GAGTC 7 cut(s) 275, 713, 1556, 2137, 2237, 2431, 2662
Ppu21I YACGTR 1 cut(s) 1006
PscI ACATGT 1 cut(s) 2792
Psp6I CCWGG 4 cut(s) 111, 1051, 2519, 2806
PspCI CACGTG 1 cut(s) 1006
PspFI CCCAGC 2 cut(s) 2058, 2207
PspGI CCWGG 4 cut(s) 111, 1051, 2519, 2806
PspN4I GGNNCC 3 cut(s) 34, 328, 1387
PspPI GGNCC 4 cut(s) 109, 327, 478, 588
PstI CTGCAG 3 cut(s) 2188, 2544, 2843
PstNI CAGNNNCTG 1 cut(s) 722
PsuI RGATCY 6 cut(s) 532, 727, 1281, 1781, 2033, 2178
PsyI GACNNNGTC 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 2576
RsaI GTAC 7 cut(s) 14, 347, 864, 879, 1872, 2068, 2126
RsaNI GTAC 7 cut(s) 13, 346, 863, 878, 1871, 2067, 2125
RseI CAYNNNNRTG 2 cut(s) 576, 1983
SacII CCGCGG 1 cut(s) 442
SaqAI TTAA 7 cut(s) 164, 663, 1530, 1961, 2406, 2600, 2624
Sau96I GGNCC 4 cut(s) 109, 327, 478, 588
SchI GAGTC 7 cut(s) 275, 713, 1557, 2138, 2237, 2432, 2662
ScrFI CCNGG 7 cut(s) 88, 107, 108, 113, 1053, 2521, 2808
SexAI ACCWGGT 1 cut(s) 1051
SfaNI GCATC 8 cut(s) 162, 435, 1627, 1927, 2418, 2443, 2707, 2824
SfcI CTRYAG 4 cut(s) 1188, 2184, 2540, 2839
Sfr303I CCGCGG 1 cut(s) 442
SfuI TTCGAA 1 cut(s) 2788
SgrBI CCGCGG 1 cut(s) 442
SinI GGWCC 2 cut(s) 478, 588
SmaI CCCGGG 1 cut(s) 108
SmiMI CAYNNNNRTG 2 cut(s) 576, 1983
SmlI CTYRAG 4 cut(s) 1157, 1785, 2087, 2168
SmoI CTYRAG 4 cut(s) 1157, 1785, 2087, 2168
SphI GCATGC 2 cut(s) 556, 2815
SsiI CCGC 9 cut(s) 36, 194, 212, 439, 441, 860, 913, 2056, 2306
SspI AATATT 1 cut(s) 2589
SspMI CTAG 4 cut(s) 1106, 1814, 2261, 2360
StyD4I CCNGG 7 cut(s) 86, 105, 106, 111, 1051, 2519, 2806
StyI CCWWGG 3 cut(s) 949, 1309, 1389
TaaI ACNGT 9 cut(s) 83, 317, 345, 482, 808, 1249, 1432, 2012, 2825
TaiI ACGT 5 cut(s) 138, 879, 1008, 1419, 2497
TaqI TCGA 6 cut(s) 600, 1048, 1746, 2426, 2788, 2818
TaqII GACCGA 1 cut(s) 2255
TatI WGTACW 1 cut(s) 1870
TauI GCSGC 3 cut(s) 38, 196, 2058
Tru1I TTAA 7 cut(s) 164, 663, 1530, 1961, 2406, 2600, 2624
Tru9I TTAA 7 cut(s) 164, 663, 1530, 1961, 2406, 2600, 2624
TscAI CASTG 5 cut(s) 253, 466, 1441, 1740, 2323
TseI GCWGC 8 cut(s) 176, 1274, 1729, 1857, 2536, 2539, 2660, 2841
TspDTI ATGAA 7 cut(s) 983, 1506, 1607, 1982, 2160, 2406, 2503
TspMI CCCGGG 1 cut(s) 106
TspRI CASTG 5 cut(s) 253, 466, 1441, 1740, 2323
Tth111I GACNNNGTC 1 cut(s) 467
Van91I CCANNNNNTGG 1 cut(s) 1298
VpaK11BI GGWCC 2 cut(s) 478, 588
XagI CCTNNNNNAGG 1 cut(s) 116
XapI RAATTY 8 cut(s) 273, 294, 515, 582, 829, 1482, 1946, 2510
XceI RCATGY 4 cut(s) 556, 843, 2796, 2815
XcmI CCANNNNNNNNNTGG 1 cut(s) 1648
XmaI CCCGGG 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 2491
XspI CTAG 4 cut(s) 1106, 1814, 2261, 2360
Zsp2I ATGCAT 1 cut(s) 2817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.