Rorug05G0218900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000636
Physical Location & Seq
Reverse (-)
22509126 .. 22512931
3806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0218900.1

Sequence Viewer

Length: 1047 bp
ATGCCTTCGTCCCCCAAGTTCTTCCACGCGCGCCAACCCCCCTCCGGTCGCCGATCGACGGTCCTGATCATCTGCTTCTGCCTCGTCTTCGGCCTCTCCGGCTTCGTCTTCGGCCTCGTCGCGCTCCGCAGCAGCCAGTACAACTGCCTCACCACCCAGTCCAGATCGGTGAGGGTCGTCTGGGAGCGAATTGGCAGCACCACCAGAACCAGCCAAAACGGCCTCGTGTTCAGCGACGGAGATGCAAATAGCGGCAGGCATAAAGTCATGGGCTTCGTCGGCATTCAGACCGGCTTCGGATCCGTCGGCCGGAGAGAGTCCCTCCGGAAGACTTGGATGCCGTCCGATGCCCAGGGCCTTCAACGATTGGAAGAAGCCACTGGTTTGGCTTTTAGGTTCATCATTGGTAAAAGCAAGGACAAAGCACAGATGTTGGAGCTGGGAAAGGAAGTGGCGAAATACGACGATTTCTTGCTGTTGGATATTGAAGAGGAGTACAGTAAGCTCCCCTACAAAACATTGGCTTTCTTCAAAGCGGCCTATGCGCTTTTTGATTCCGACTTCTATGTTAAAGCTGACGACGACATTTACTTGAGGCCAGATCGCCTTTCGACGCTCTTGGCAAAAGAGCGCTCTCACTCTCAGACTTACCTTGGATGCATGAAAAAGGGCCCAGTCTTCACCGACCCAAAGCTTAAATGGTTTGAGCCACTAGCGTATTTGCTAGGGAGTGAATACTTTCTCCATGCCTACGGTCCAATCTATGCTCTTTCTGCCGATGTTGTTGCAAGTTTGGTTGCTCTTAGGAATAACAGTTTCCGGATGTTTAGCAATGAGGATGTCACAATTGGTGCGTGGATGCTTGCAATGAACGTCAACCATGAGAACAATCAAGCACTATGCTCACCAGAATGTACATCATCATCTATTGCTGTGTGGGATATTCCTAAGTGCTCCGGGCTCTGCAATCCAGAAAAGAAACTGTTAGAACTACATCAAAAGGACAGCTGTACAAAAAGTCCAACGATGGAATCTGATGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

348

Amino Acids

39.0

Weight (kDa)

8.0

Isoelectric Point (pI)

52.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Galactosyl_T PF01762 104 - 297 1.7e-48 Galactosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000636)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36290 FvH4_5g27770 FvH4_5g29860 FvH4_5g37342 FvH4_5g37877 FvH4_5g37880 FvH4_5g37900 FvH4_6g40020
rosa_chinensis RchiOBHm_Chr7g0227221 RchiOBHm_Chr7g0239011
rosa_multiflora Rmu_co8202712.1_g000001 Rmu_sc0000477.1_g000006 Rmu_sc0001047.1_g000050 Rmu_sc0001371.1_g000021 Rmu_sc0002449.1_g000018 Rmu_sc0002988.1_g000003 Rmu_sc0003089.1_g000031 Rmu_sc0004062.1_g000013 Rmu_sc0004132.1_g000001 Rmu_sc0004277.1_g000045 Rmu_sc0004567.1_g000017 Rmu_sc0005327.1_g000024 Rmu_sc0005651.1_g000005 Rmu_sc0006669.1_g000014 Rmu_sc0018004.1_g000002 Rmu_sc0018978.1_g000001 Rmu_sc0021209.1_g000001 Rmu_sc0034369.1_g000001 Rmu_ssc0000398.1_g000019
rosa_roxburghii Rroxscaffold_1G00004410 Rroxscaffold_3G00220090 Rroxscaffold_3G00221380 Rroxscaffold_3G00222090 Rroxscaffold_3G00223100 Rroxscaffold_6G00400160 Rroxscaffold_7G00204490 Rroxscaffold_7G00204500
rosa_rugosa Rorug02G0147900 Rorug03G0117000 Rorug03G0194000 Rorug04G0112500 Rorug05G0218600 Rorug05G0218700 Rorug05G0218800 Rorug05G0218900 Rorug05G0219000 Rorug05G0219000 Rorug05G0219000 Rorug05G0219100 Rorug05G0220000 Rorug05G0574900 Rorug07G0311900 Rorug07G0312000 Rorug07G0324500 Rorug07G0324600
rosa_samantha Rh3BG280100 Rh4BG071800 Rh5BG306100 Rh5BG308200 Rh5BG313000 Rh5BG335200 Rh5BG532100 Rh5BG532200 Rh5CG556100 Rh5DG315400 Rh5DG318300 Rh5DG318400 Rh5DG347500 Rh6CG116800 Rh7BG420800 Rh7BG437600 Rh7BG452200 Rh7CG484800 Rh7CG496900 Rh7DG453200 Rh7DG464300
rosa_wichuraiana Rw7G039600 Rw7G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 29, 31, 122
AccIII TCCGGA 2 cut(s) 324, 819
AciI CCGC 3 cut(s) 127, 252, 536
AclWI GGATC 2 cut(s) 294, 307
AcoI YGGCCR 1 cut(s) 307
AfaI GTAC 4 cut(s) 140, 497, 916, 1012
AfeI AGCGCT 1 cut(s) 632
AfiI CCNNNNNNNGG 3 cut(s) 44, 58, 309
AgsI TTSAA 3 cut(s) 362, 488, 532
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 5 cut(s) 439, 505, 575, 694, 1008
AluI AGCT 5 cut(s) 439, 505, 575, 694, 1008
Alw21I GWGCWC 1 cut(s) 956
AlwI GGATC 2 cut(s) 294, 307
Aor13HI TCCGGA 2 cut(s) 324, 819
Aor51HI AGCGCT 1 cut(s) 632
AoxI GGCC 8 cut(s) 91, 112, 220, 307, 355, 537, 596, 670
ApaI GGGCCC 1 cut(s) 674
ApeKI GCWGC 3 cut(s) 129, 132, 195
Asp700I GAANNNNTTC 1 cut(s) 738
AspLEI GCGC 5 cut(s) 31, 33, 124, 547, 633
AspS9I GGNCC 5 cut(s) 61, 355, 670, 671, 755
AsuC2I CCSGG 1 cut(s) 958
AsuHPI GGTGA 4 cut(s) 142, 181, 673, 897
AvaII GGWCC 2 cut(s) 61, 755
BaeGI GKGCMC 1 cut(s) 674
BamHI GGATCC 1 cut(s) 299
BanII GRGCYC 2 cut(s) 674, 963
BauI CACGAG 1 cut(s) 224
BbsI GAAGAC 4 cut(s) 79, 100, 335, 670
Bbv12I GWGCWC 1 cut(s) 956
BbvI GCAGC 3 cut(s) 141, 144, 207
BccI CCATC 1 cut(s) 1021
BceAI ACGGC 2 cut(s) 235, 325
BcgI CGANNNNNNTGC 4 cut(s) 224, 258, 767, 801
BciT130I CCWGG 1 cut(s) 353
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 1 cut(s) 958
BfaI CTAG 2 cut(s) 713, 725
BfoI RGCGCY 1 cut(s) 634
BglI GCCNNNNNGGC 2 cut(s) 99, 219
BisI GCNGC 5 cut(s) 130, 133, 196, 253, 537
BlsI GCNGC 5 cut(s) 131, 134, 197, 254, 538
Bme1390I CCNGG 2 cut(s) 353, 958
Bme18I GGWCC 2 cut(s) 61, 755
BmgT120I GGNCC 5 cut(s) 61, 355, 670, 671, 755
BmiI GGNNCC 2 cut(s) 301, 672
BmrFI CCNGG 2 cut(s) 353, 958
BmrI ACTGGG 2 cut(s) 151, 668
BmsI GCATC 5 cut(s) 232, 327, 337, 647, 849
BmuI ACTGGG 2 cut(s) 151, 668
BpiI GAAGAC 4 cut(s) 79, 100, 335, 670
BplI GAGNNNNNCTC 2 cut(s) 306, 338
BpuEI CTTGAG 1 cut(s) 613
BpuMI CCSGG 1 cut(s) 958
BsaJI CCNNGG 3 cut(s) 351, 352, 652
BsaWI WCCGGW 3 cut(s) 44, 324, 819
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 58, 309
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 4 cut(s) 136, 157, 385, 674
Bse3DI GCAATG 2 cut(s) 838, 873
BseAI TCCGGA 2 cut(s) 324, 819
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 3 cut(s) 351, 352, 652
BseGI GGATG 5 cut(s) 342, 662, 828, 844, 864
BseLI CCNNNNNNNGG 3 cut(s) 44, 58, 309
BseMI GCAATG 2 cut(s) 838, 873
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 4 cut(s) 136, 157, 385, 674
BsePI GCGCGC 1 cut(s) 29
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 674
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 3 cut(s) 141, 144, 207
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 3 cut(s) 29, 31, 122
Bsh1285I CGRYCG 3 cut(s) 49, 56, 310
BshFI GGCC 8 cut(s) 93, 114, 222, 309, 357, 539, 598, 672
BsiEI CGRYCG 3 cut(s) 49, 56, 310
BsiHKAI GWGCWC 1 cut(s) 956
BsiSI CCGG 7 cut(s) 45, 99, 291, 310, 325, 820, 957
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 3 cut(s) 44, 58, 309
BsmFI GGGAC 1 cut(s) 304
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 8 cut(s) 93, 114, 222, 309, 357, 539, 598, 672
Bsp120I GGGCCC 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 674, 956, 963
Bsp13I TCCGGA 2 cut(s) 324, 819
Bsp1407I TGTACA 2 cut(s) 914, 1010
Bsp143I GATC 5 cut(s) 53, 66, 164, 299, 601
BspACI CCGC 3 cut(s) 127, 252, 536
BspANI GGCC 8 cut(s) 93, 114, 222, 309, 357, 539, 598, 672
BspCNI CTCAG 1 cut(s) 655
BspEI TCCGGA 2 cut(s) 324, 819
BspFNI CGCG 3 cut(s) 29, 31, 122
BspLI GGNNCC 2 cut(s) 301, 672
BspPI GGATC 2 cut(s) 294, 307
BsrDI GCAATG 2 cut(s) 838, 873
BsrFI RCCGGY 1 cut(s) 290
BsrGI TGTACA 2 cut(s) 914, 1010
BsrI ACTGG 4 cut(s) 136, 157, 385, 674
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 3 cut(s) 351, 352, 652
BssHII GCGCGC 1 cut(s) 29
BssMI GATC 5 cut(s) 53, 66, 164, 299, 601
BssSI CACGAG 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 652
Bst2BI CACGAG 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 5 cut(s) 61, 500, 755, 815, 984
Bst6I CTCTTC 1 cut(s) 483
BstAUI TGTACA 2 cut(s) 914, 1010
BstC8I GCNNGC 3 cut(s) 31, 257, 864
BstDEI CTNAG 3 cut(s) 642, 803, 948
BstF5I GGATG 5 cut(s) 342, 662, 828, 844, 864
BstFNI CGCG 3 cut(s) 29, 31, 122
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 5 cut(s) 31, 33, 124, 547, 633
BstKTI GATC 5 cut(s) 56, 69, 167, 302, 604
BstMBI GATC 5 cut(s) 53, 66, 164, 299, 601
BstMCI CGRYCG 3 cut(s) 49, 56, 310
BstMWI GCNNNNNNNGC 5 cut(s) 99, 219, 279, 542, 773
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 351, 956
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 3 cut(s) 29, 31, 122
BstV1I GCAGC 3 cut(s) 141, 144, 207
BstV2I GAAGAC 4 cut(s) 79, 100, 335, 670
BstX2I RGATCY 1 cut(s) 299
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 1 cut(s) 299
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 8 cut(s) 93, 114, 222, 309, 357, 539, 598, 672
BtsCI GGATG 5 cut(s) 342, 662, 828, 844, 864
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 31, 257, 864
CfoI GCGC 5 cut(s) 31, 33, 124, 547, 633
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 5 cut(s) 61, 355, 670, 671, 755
CseI GACGC 1 cut(s) 622
Csp6I GTAC 4 cut(s) 139, 496, 915, 1011
CviAII CATG 4 cut(s) 268, 661, 746, 881
CviQI GTAC 4 cut(s) 139, 496, 915, 1011
DdeI CTNAG 3 cut(s) 642, 803, 948
DpnI GATC 5 cut(s) 55, 68, 166, 301, 603
DpnII GATC 5 cut(s) 53, 66, 164, 299, 601
EaeI YGGCCR 1 cut(s) 307
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 652
Eco24I GRGCYC 2 cut(s) 674, 963
Eco47I GGWCC 2 cut(s) 61, 755
Eco47III AGCGCT 1 cut(s) 632
Eco52I CGGCCG 1 cut(s) 307
EcoO109I RGGNCCY 2 cut(s) 355, 670
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 1 cut(s) 652
EcoT22I ATGCAT 1 cut(s) 662
EcoT38I GRGCYC 2 cut(s) 674, 963
ErhI CCWWGG 1 cut(s) 652
FaeI CATG 4 cut(s) 271, 664, 749, 884
FaiI YATR 9 cut(s) 261, 269, 543, 567, 662, 747, 765, 882, 901
FaqI GGGAC 1 cut(s) 304
FatI CATG 4 cut(s) 267, 660, 745, 880
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 130, 133, 196, 253, 537
FokI GGATG 5 cut(s) 349, 669, 835, 851, 871
FriOI GRGCYC 2 cut(s) 674, 963
Fsp4HI GCNGC 5 cut(s) 130, 133, 196, 253, 537
FspBI CTAG 2 cut(s) 713, 725
GlaI GCGC 5 cut(s) 30, 32, 123, 546, 632
GluI GCNGC 5 cut(s) 130, 133, 196, 253, 537
GsaI CCCAGC 1 cut(s) 443
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 8 cut(s) 93, 114, 222, 309, 357, 539, 598, 672
HapII CCGG 7 cut(s) 45, 99, 291, 310, 325, 820, 957
HgaI GACGC 1 cut(s) 622
HhaI GCGC 5 cut(s) 31, 33, 124, 547, 633
Hin1II CATG 4 cut(s) 271, 664, 749, 884
Hin6I GCGC 5 cut(s) 29, 31, 122, 545, 631
HinP1I GCGC 5 cut(s) 29, 31, 122, 545, 631
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 3 cut(s) 317, 554, 1031
HpaII CCGG 7 cut(s) 45, 99, 291, 310, 325, 820, 957
HphI GGTGA 4 cut(s) 142, 181, 673, 897
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 6 cut(s) 288, 299, 346, 559, 645, 1036
Hpy188III TCNNGA 5 cut(s) 64, 162, 325, 820, 971
Hpy8I GTNNAC 1 cut(s) 877
Hpy99I CGWCG 8 cut(s) 61, 122, 239, 281, 308, 467, 584, 616
HpyAV CCTTC 2 cut(s) 15, 368
HpyCH4III ACNGT 5 cut(s) 61, 500, 755, 815, 984
HpyCH4IV ACGT 1 cut(s) 873
HpyCH4V TGCA 5 cut(s) 245, 660, 788, 866, 966
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 219, 279, 542, 773
HpyF3I CTNAG 3 cut(s) 642, 803, 948
HpySE526I ACGT 1 cut(s) 873
Hsp92II CATG 4 cut(s) 271, 664, 749, 884
HspAI GCGC 5 cut(s) 29, 31, 122, 545, 631
Kpn2I TCCGGA 2 cut(s) 324, 819
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 5 cut(s) 53, 66, 164, 299, 601
LmnI GCTCC 5 cut(s) 129, 184, 436, 510, 959
Lsp1109I GCAGC 3 cut(s) 141, 144, 207
LweI GCATC 5 cut(s) 232, 327, 337, 647, 849
MaeI CTAG 2 cut(s) 713, 725
MaeII ACGT 1 cut(s) 873
MaeIII GTNAC 1 cut(s) 841
MalI GATC 5 cut(s) 55, 68, 166, 301, 603
MboI GATC 5 cut(s) 53, 66, 164, 299, 601
MboII GAAGA 8 cut(s) 13, 79, 100, 340, 383, 500, 520, 670
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 1 cut(s) 299
MhlI GDGCHC 3 cut(s) 674, 956, 963
MluCI AATT 2 cut(s) 189, 846
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 4 cut(s) 414, 459, 582, 1046
Mph1103I ATGCAT 1 cut(s) 662
MroI TCCGGA 2 cut(s) 324, 819
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 2 cut(s) 570, 696
MslI CAYNNNNRTG 1 cut(s) 910
MspA1I CMGCKG 1 cut(s) 1008
MspI CCGG 7 cut(s) 45, 99, 291, 310, 325, 820, 957
MspR9I CCNGG 2 cut(s) 353, 958
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 1 cut(s) 353
MvnI CGCG 3 cut(s) 29, 31, 122
MwoI GCNNNNNNNGC 5 cut(s) 99, 219, 279, 542, 773
NciI CCSGG 1 cut(s) 958
NdeII GATC 5 cut(s) 53, 66, 164, 299, 601
NlaIII CATG 4 cut(s) 271, 664, 749, 884
NlaIV GGNNCC 2 cut(s) 301, 672
NmuCI GTSAC 1 cut(s) 841
NsiI ATGCAT 1 cut(s) 662
PasI CCCWGGG 1 cut(s) 352
PauI GCGCGC 1 cut(s) 29
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 2 cut(s) 554, 1031
PkrI GCNGC 5 cut(s) 131, 134, 197, 254, 538
Ple19I CGATCG 1 cut(s) 56
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 351
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 301, 672
PspOMI GGGCCC 1 cut(s) 670
PspPI GGNCC 5 cut(s) 61, 355, 670, 671, 755
PsuI RGATCY 1 cut(s) 299
PteI GCGCGC 1 cut(s) 29
PvuI CGATCG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 1008
RsaI GTAC 4 cut(s) 140, 497, 916, 1012
RsaNI GTAC 4 cut(s) 139, 496, 915, 1011
RseI CAYNNNNRTG 1 cut(s) 910
SaqAI TTAA 2 cut(s) 570, 696
SatI GCNGC 5 cut(s) 130, 133, 196, 253, 537
Sau3AI GATC 5 cut(s) 53, 66, 164, 299, 601
Sau96I GGNCC 5 cut(s) 61, 355, 670, 671, 755
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 2 cut(s) 353, 958
SduI GDGCHC 3 cut(s) 674, 956, 963
SetI ASST 8 cut(s) 398, 441, 507, 577, 654, 696, 876, 1010
SfaNI GCATC 5 cut(s) 232, 327, 337, 647, 849
SinI GGWCC 2 cut(s) 61, 755
SmiMI CAYNNNNRTG 1 cut(s) 910
SmlI CTYRAG 1 cut(s) 592
SmoI CTYRAG 1 cut(s) 592
Sse9I AATT 2 cut(s) 189, 846
SsiI CCGC 3 cut(s) 127, 252, 536
SspMI CTAG 2 cut(s) 713, 725
StyD4I CCNGG 2 cut(s) 351, 956
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 5 cut(s) 61, 500, 755, 815, 984
TaiI ACGT 1 cut(s) 876
TaqI TCGA 2 cut(s) 56, 611
TasI AATT 2 cut(s) 189, 846
TatI WGTACW 4 cut(s) 138, 495, 914, 1010
TauI GCSGC 2 cut(s) 255, 539
TfiI GAWTC 2 cut(s) 554, 1031
Tru1I TTAA 2 cut(s) 570, 696
Tru9I TTAA 2 cut(s) 570, 696
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 3 cut(s) 129, 132, 195
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 3 cut(s) 388, 677, 884
TspGWI ACGGA 2 cut(s) 252, 292
TspRI CASTG 1 cut(s) 385
VpaK11BI GGWCC 2 cut(s) 61, 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 696
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 2 cut(s) 713, 725
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.