Belongs to the sterol desaturase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 293 |
| AatII | GACGTC | 1 cut(s) | 882 |
| Acc16I | TGCGCA | 1 cut(s) | 1052 |
| Acc65I | GGTACC | 1 cut(s) | 981 |
| AccB1I | GGYRCC | 2 cut(s) | 344, 981 |
| AccI | GTMKAC | 1 cut(s) | 1508 |
| AccII | CGCG | 1 cut(s) | 1452 |
| AccIII | TCCGGA | 1 cut(s) | 765 |
| AciI | CCGC | 3 cut(s) | 1622, 1636, 1794 |
| AclWI | GGATC | 3 cut(s) | 200, 592, 605 |
| AcoI | YGGCCR | 1 cut(s) | 32 |
| AcuI | CTGAAG | 1 cut(s) | 1020 |
| AcyI | GRCGYC | 5 cut(s) | 5, 345, 584, 879, 1431 |
| AfaI | GTAC | 5 cut(s) | 62, 104, 983, 1268, 1503 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 70, 214, 366, 594 |
| AgsI | TTSAA | 4 cut(s) | 58, 304, 1187, 1291 |
| AhlI | ACTAGT | 1 cut(s) | 1300 |
| AjiI | CACGTC | 1 cut(s) | 516 |
| AjnI | CCWGG | 1 cut(s) | 340 |
| AleI | CACNNNNGTG | 2 cut(s) | 518, 1391 |
| Alw21I | GWGCWC | 4 cut(s) | 22, 445, 528, 543 |
| Alw26I | GTCTC | 5 cut(s) | 207, 695, 972, 1082, 1823 |
| Alw44I | GTGCAC | 1 cut(s) | 524 |
| AlwI | GGATC | 3 cut(s) | 200, 592, 605 |
| Aor13HI | TCCGGA | 1 cut(s) | 765 |
| AoxI | GGCC | 6 cut(s) | 11, 32, 72, 378, 1001, 1222 |
| ApaLI | GTGCAC | 1 cut(s) | 524 |
| ApeKI | GCWGC | 3 cut(s) | 1314, 1322, 1838 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 808, 840 |
| Asp718I | GGTACC | 1 cut(s) | 981 |
| AspLEI | GCGC | 5 cut(s) | 279, 347, 930, 1053, 1143 |
| AspS9I | GGNCC | 7 cut(s) | 12, 73, 268, 378, 407, 768, 1002 |
| AsuC2I | CCSGG | 1 cut(s) | 15 |
| AsuHPI | GGTGA | 9 cut(s) | 94, 473, 493, 864, 887, 892, 1081, 1094, 1741 |
| AvaII | GGWCC | 3 cut(s) | 268, 407, 768 |
| BaeGI | GKGCMC | 1 cut(s) | 528 |
| BalI | TGGCCA | 1 cut(s) | 34 |
| BamHI | GGATCC | 1 cut(s) | 597 |
| BanI | GGYRCC | 2 cut(s) | 344, 981 |
| BanII | GRGCYC | 3 cut(s) | 22, 445, 510 |
| Bbv12I | GWGCWC | 4 cut(s) | 22, 445, 528, 543 |
| BbvCI | CCTCAGC | 1 cut(s) | 134 |
| BbvI | GCAGC | 3 cut(s) | 1326, 1334, 1825 |
| BccI | CCATC | 9 cut(s) | 79, 389, 518, 614, 623, 923, 1163, 1547, 1879 |
| BceAI | ACGGC | 3 cut(s) | 126, 231, 597 |
| BciT130I | CCWGG | 1 cut(s) | 342 |
| BciVI | GTATCC | 2 cut(s) | 617, 1290 |
| BcnI | CCSGG | 1 cut(s) | 15 |
| BcoDI | GTCTC | 5 cut(s) | 207, 695, 972, 1082, 1823 |
| BcuI | ACTAGT | 1 cut(s) | 1300 |
| BfaI | CTAG | 2 cut(s) | 371, 1301 |
| BfoI | RGCGCY | 2 cut(s) | 348, 1144 |
| BfuI | GTATCC | 2 cut(s) | 617, 1290 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1428 |
| BisI | GCNGC | 4 cut(s) | 1315, 1323, 1795, 1839 |
| BlsI | GCNGC | 4 cut(s) | 1316, 1324, 1796, 1840 |
| Bme1390I | CCNGG | 2 cut(s) | 15, 342 |
| Bme18I | GGWCC | 3 cut(s) | 268, 407, 768 |
| BmgBI | CACGTC | 1 cut(s) | 516 |
| BmgT120I | GGNCC | 7 cut(s) | 12, 73, 268, 378, 407, 768, 1002 |
| BmiI | GGNNCC | 7 cut(s) | 75, 346, 379, 409, 599, 983, 1594 |
| BmrFI | CCNGG | 2 cut(s) | 15, 342 |
| BmsI | GCATC | 1 cut(s) | 150 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1368, 1400 |
| BpmI | CTGGAG | 2 cut(s) | 1278, 1647 |
| Bpu10I | CCTNAGC | 3 cut(s) | 134, 219, 684 |
| BpuEI | CTTGAG | 2 cut(s) | 698, 1790 |
| BpuMI | CCSGG | 1 cut(s) | 15 |
| BsaAI | YACGTR | 2 cut(s) | 106, 1270 |
| BsaBI | GATNNNNATC | 1 cut(s) | 191 |
| BsaHI | GRCGYC | 5 cut(s) | 5, 345, 584, 879, 1431 |
| BsaI | GGTCTC | 1 cut(s) | 972 |
| BsaJI | CCNNGG | 7 cut(s) | 35, 46, 340, 362, 931, 977, 1359 |
| BsaWI | WCCGGW | 1 cut(s) | 765 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 473, 476, 503, 506, 1685, 1715, 1743, 1773 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 70, 214, 366, 594 |
| Bse1I | ACTGG | 3 cut(s) | 434, 1654, 1718 |
| Bse3DI | GCAATG | 1 cut(s) | 661 |
| Bse8I | GATNNNNATC | 1 cut(s) | 191 |
| BseAI | TCCGGA | 1 cut(s) | 765 |
| BseBI | CCWGG | 1 cut(s) | 342 |
| BseDI | CCNNGG | 7 cut(s) | 35, 46, 340, 362, 931, 977, 1359 |
| BseGI | GGATG | 4 cut(s) | 124, 381, 882, 1558 |
| BseJI | GATNNNNATC | 1 cut(s) | 191 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 70, 214, 366, 594 |
| BseMI | GCAATG | 1 cut(s) | 661 |
| BseMII | CTCAG | 1 cut(s) | 148 |
| BseNI | ACTGG | 3 cut(s) | 434, 1654, 1718 |
| BseRI | GAGGAG | 9 cut(s) | 137, 302, 481, 940, 1004, 1469, 1738, 1796, 1866 |
| BseSI | GKGCMC | 1 cut(s) | 528 |
| BseXI | GCAGC | 3 cut(s) | 1326, 1334, 1825 |
| BseYI | CCCAGC | 1 cut(s) | 1717 |
| Bsh1236I | CGCG | 1 cut(s) | 1452 |
| BshFI | GGCC | 6 cut(s) | 13, 34, 74, 380, 1003, 1224 |
| BshNI | GGYRCC | 2 cut(s) | 344, 981 |
| BsiHKAI | GWGCWC | 4 cut(s) | 22, 445, 528, 543 |
| BsiSI | CCGG | 2 cut(s) | 15, 766 |
| BsiWI | CGTACG | 1 cut(s) | 102 |
| BslFI | GGGAC | 7 cut(s) | 27, 299, 393, 571, 672, 826, 928 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 70, 214, 366, 594 |
| BsmAI | GTCTC | 5 cut(s) | 207, 695, 972, 1082, 1823 |
| BsmBI | CGTCTC | 3 cut(s) | 695, 1082, 1823 |
| BsmFI | GGGAC | 7 cut(s) | 27, 299, 393, 571, 672, 826, 928 |
| BsnI | GGCC | 6 cut(s) | 13, 34, 74, 380, 1003, 1224 |
| Bso31I | GGTCTC | 1 cut(s) | 972 |
| Bsp1286I | GDGCHC | 5 cut(s) | 22, 445, 510, 528, 543 |
| Bsp13I | TCCGGA | 1 cut(s) | 765 |
| Bsp143I | GATC | 3 cut(s) | 192, 234, 597 |
| Bsp19I | CCATGG | 3 cut(s) | 35, 362, 977 |
| BspACI | CCGC | 3 cut(s) | 1622, 1636, 1794 |
| BspANI | GGCC | 6 cut(s) | 13, 34, 74, 380, 1003, 1224 |
| BspCNI | CTCAG | 1 cut(s) | 147 |
| BspEI | TCCGGA | 1 cut(s) | 765 |
| BspFNI | CGCG | 1 cut(s) | 1452 |
| BspHI | TCATGA | 1 cut(s) | 645 |
| BspLI | GGNNCC | 7 cut(s) | 75, 346, 379, 409, 599, 983, 1594 |
| BspPI | GGATC | 3 cut(s) | 200, 592, 605 |
| BspQI | GCTCTTC | 2 cut(s) | 1563, 1848 |
| BspT107I | GGYRCC | 2 cut(s) | 344, 981 |
| BspTNI | GGTCTC | 1 cut(s) | 972 |
| BsrDI | GCAATG | 1 cut(s) | 661 |
| BsrI | ACTGG | 3 cut(s) | 434, 1654, 1718 |
| BssECI | CCNNGG | 7 cut(s) | 35, 46, 340, 362, 931, 977, 1359 |
| BssMI | GATC | 3 cut(s) | 192, 234, 597 |
| BssNI | GRCGYC | 5 cut(s) | 5, 345, 584, 879, 1431 |
| BssT1I | CCWWGG | 5 cut(s) | 35, 46, 362, 931, 977 |
| Bst2UI | CCWGG | 1 cut(s) | 342 |
| Bst4CI | ACNGT | 5 cut(s) | 1076, 1093, 1330, 1506, 1646 |
| Bst6I | CTCTTC | 4 cut(s) | 698, 707, 1563, 1848 |
| BstACI | GRCGYC | 5 cut(s) | 5, 345, 584, 879, 1431 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1803 |
| BstBAI | YACGTR | 2 cut(s) | 106, 1270 |
| BstC8I | GCNNGC | 3 cut(s) | 91, 95, 1764 |
| BstDEI | CTNAG | 3 cut(s) | 134, 219, 684 |
| BstDSI | CCRYGG | 4 cut(s) | 35, 362, 977, 1359 |
| BstF5I | GGATG | 4 cut(s) | 124, 381, 882, 1558 |
| BstFNI | CGCG | 1 cut(s) | 1452 |
| BstH2I | RGCGCY | 2 cut(s) | 348, 1144 |
| BstHHI | GCGC | 5 cut(s) | 279, 347, 930, 1053, 1143 |
| BstKTI | GATC | 3 cut(s) | 195, 237, 600 |
| BstMAI | GTCTC | 5 cut(s) | 207, 695, 972, 1082, 1823 |
| BstMBI | GATC | 3 cut(s) | 192, 234, 597 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 204, 1428, 1803 |
| BstNI | CCWGG | 1 cut(s) | 342 |
| BstNSI | RCATGY | 3 cut(s) | 97, 993, 1766 |
| BstSCI | CCNGG | 2 cut(s) | 13, 340 |
| BstSLI | GKGCMC | 1 cut(s) | 528 |
| BstUI | CGCG | 1 cut(s) | 1452 |
| BstV1I | GCAGC | 3 cut(s) | 1326, 1334, 1825 |
| BstX2I | RGATCY | 1 cut(s) | 597 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 388, 574, 1539 |
| BstYI | RGATCY | 1 cut(s) | 597 |
| BsuI | GTATCC | 2 cut(s) | 617, 1290 |
| BsuRI | GGCC | 6 cut(s) | 13, 34, 74, 380, 1003, 1224 |
| BtgI | CCRYGG | 4 cut(s) | 35, 362, 977, 1359 |
| BtgZI | GCGATG | 1 cut(s) | 529 |
| BtrI | CACGTC | 1 cut(s) | 516 |
| BtsCI | GGATG | 4 cut(s) | 124, 381, 882, 1558 |
| Cac8I | GCNNGC | 3 cut(s) | 91, 95, 1764 |
| CciI | TCATGA | 1 cut(s) | 645 |
| CfoI | GCGC | 5 cut(s) | 279, 347, 930, 1053, 1143 |
| Cfr13I | GGNCC | 7 cut(s) | 12, 73, 268, 378, 407, 768, 1002 |
| CseI | GACGC | 3 cut(s) | 573, 677, 1420 |
| Csp6I | GTAC | 5 cut(s) | 61, 103, 982, 1267, 1502 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1348, 1383 |
| CviQI | GTAC | 5 cut(s) | 61, 103, 982, 1267, 1502 |
| DdeI | CTNAG | 3 cut(s) | 134, 219, 684 |
| DinI | GGCGCC | 1 cut(s) | 346 |
| DpnI | GATC | 3 cut(s) | 194, 236, 599 |
| DpnII | GATC | 3 cut(s) | 192, 234, 597 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 293 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 293 |
| EaeI | YGGCCR | 1 cut(s) | 32 |
| Eam1104I | CTCTTC | 4 cut(s) | 698, 707, 1563, 1848 |
| EarI | CTCTTC | 4 cut(s) | 698, 707, 1563, 1848 |
| EciI | GGCGGA | 1 cut(s) | 1611 |
| Ecl136II | GAGCTC | 2 cut(s) | 20, 443 |
| Eco130I | CCWWGG | 5 cut(s) | 35, 46, 362, 931, 977 |
| Eco24I | GRGCYC | 3 cut(s) | 22, 445, 510 |
| Eco31I | GGTCTC | 1 cut(s) | 972 |
| Eco47I | GGWCC | 3 cut(s) | 268, 407, 768 |
| Eco53kI | GAGCTC | 2 cut(s) | 20, 443 |
| Eco57I | CTGAAG | 1 cut(s) | 1020 |
| EcoICRI | GAGCTC | 2 cut(s) | 20, 443 |
| EcoRII | CCWGG | 1 cut(s) | 340 |
| EcoT14I | CCWWGG | 5 cut(s) | 35, 46, 362, 931, 977 |
| EcoT38I | GRGCYC | 3 cut(s) | 22, 445, 510 |
| EgeI | GGCGCC | 1 cut(s) | 346 |
| EheI | GGCGCC | 1 cut(s) | 346 |
| ErhI | CCWWGG | 5 cut(s) | 35, 46, 362, 931, 977 |
| Esp3I | CGTCTC | 3 cut(s) | 695, 1082, 1823 |
| FaqI | GGGAC | 7 cut(s) | 27, 299, 393, 571, 672, 826, 928 |
| FauNDI | CATATG | 1 cut(s) | 1829 |
| FblI | GTMKAC | 1 cut(s) | 1508 |
| Fnu4HI | GCNGC | 4 cut(s) | 1315, 1323, 1795, 1839 |
| FokI | GGATG | 4 cut(s) | 131, 368, 869, 1565 |
| FriOI | GRGCYC | 3 cut(s) | 22, 445, 510 |
| Fsp4HI | GCNGC | 4 cut(s) | 1315, 1323, 1795, 1839 |
| FspBI | CTAG | 2 cut(s) | 371, 1301 |
| FspI | TGCGCA | 1 cut(s) | 1052 |
| GlaI | GCGC | 5 cut(s) | 278, 346, 929, 1052, 1142 |
| GluI | GCNGC | 4 cut(s) | 1315, 1323, 1795, 1839 |
| GsaI | CCCAGC | 1 cut(s) | 1721 |
| GsuI | CTGGAG | 2 cut(s) | 1278, 1647 |
| HaeII | RGCGCY | 2 cut(s) | 348, 1144 |
| HaeIII | GGCC | 6 cut(s) | 13, 34, 74, 380, 1003, 1224 |
| HapII | CCGG | 2 cut(s) | 15, 766 |
| HgaI | GACGC | 3 cut(s) | 573, 677, 1420 |
| HhaI | GCGC | 5 cut(s) | 279, 347, 930, 1053, 1143 |
| Hin1I | GRCGYC | 5 cut(s) | 5, 345, 584, 879, 1431 |
| Hin6I | GCGC | 5 cut(s) | 277, 345, 928, 1051, 1141 |
| HinP1I | GCGC | 5 cut(s) | 277, 345, 928, 1051, 1141 |
| HincII | GTYRAC | 1 cut(s) | 1489 |
| HindII | GTYRAC | 1 cut(s) | 1489 |
| HinfI | GANTC | 7 cut(s) | 40, 833, 869, 1151, 1216, 1495, 1700 |
| HpaII | CCGG | 2 cut(s) | 15, 766 |
| HphI | GGTGA | 9 cut(s) | 94, 473, 493, 864, 887, 892, 1081, 1094, 1741 |
| Hpy166II | GTNNAC | 9 cut(s) | 178, 241, 268, 526, 1096, 1306, 1489, 1509, 1659 |
| Hpy188I | TCNGA | 1 cut(s) | 1284 |
| Hpy188III | TCNNGA | 4 cut(s) | 646, 766, 969, 1257 |
| Hpy8I | GTNNAC | 9 cut(s) | 178, 241, 268, 526, 1096, 1306, 1489, 1509, 1659 |
| Hpy99I | CGWCG | 3 cut(s) | 10, 416, 881 |
| HpyAV | CCTTC | 5 cut(s) | 365, 541, 1015, 1118, 1583 |
| HpyCH4III | ACNGT | 5 cut(s) | 1076, 1093, 1330, 1506, 1646 |
| HpyCH4IV | ACGT | 5 cut(s) | 105, 515, 879, 1269, 1809 |
| HpyCH4V | TGCA | 8 cut(s) | 97, 526, 666, 1273, 1739, 1762, 1766, 1806 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 204, 1428, 1803 |
| HpyF3I | CTNAG | 3 cut(s) | 134, 219, 684 |
| HpySE526I | ACGT | 5 cut(s) | 105, 515, 879, 1269, 1809 |
| Hsp92I | GRCGYC | 5 cut(s) | 5, 345, 584, 879, 1431 |
| HspAI | GCGC | 5 cut(s) | 277, 345, 928, 1051, 1141 |
| KasI | GGCGCC | 1 cut(s) | 344 |
| Kpn2I | TCCGGA | 1 cut(s) | 765 |
| KpnI | GGTACC | 1 cut(s) | 985 |
| Kzo9I | GATC | 3 cut(s) | 192, 234, 597 |
| LguI | GCTCTTC | 2 cut(s) | 1563, 1848 |
| LmnI | GCTCC | 5 cut(s) | 17, 342, 753, 1311, 1783 |
| Lsp1109I | GCAGC | 3 cut(s) | 1326, 1334, 1825 |
| LweI | GCATC | 1 cut(s) | 150 |
| MaeI | CTAG | 2 cut(s) | 371, 1301 |
| MaeII | ACGT | 5 cut(s) | 105, 515, 879, 1269, 1809 |
| MaeIII | GTNAC | 6 cut(s) | 106, 499, 577, 725, 1082, 1446 |
| MalI | GATC | 3 cut(s) | 194, 236, 599 |
| MboI | GATC | 3 cut(s) | 192, 234, 597 |
| MboII | GAAGA | 7 cut(s) | 292, 685, 694, 878, 1303, 1580, 1835 |
| MflI | RGATCY | 1 cut(s) | 597 |
| MhlI | GDGCHC | 5 cut(s) | 22, 445, 510, 528, 543 |
| MlsI | TGGCCA | 1 cut(s) | 34 |
| MluCI | AATT | 2 cut(s) | 823, 1365 |
| MluNI | TGGCCA | 1 cut(s) | 34 |
| Mly113I | GGCGCC | 1 cut(s) | 345 |
| MlyI | GAGTC | 5 cut(s) | 49, 827, 1160, 1225, 1694 |
| MmeI | TCCRAC | 2 cut(s) | 834, 1882 |
| Mox20I | TGGCCA | 1 cut(s) | 34 |
| MroI | TCCGGA | 1 cut(s) | 765 |
| MscI | TGGCCA | 1 cut(s) | 34 |
| MslI | CAYNNNNRTG | 4 cut(s) | 386, 518, 572, 1391 |
| Msp20I | TGGCCA | 1 cut(s) | 34 |
| MspI | CCGG | 2 cut(s) | 15, 766 |
| MspR9I | CCNGG | 2 cut(s) | 15, 342 |
| MvaI | CCWGG | 1 cut(s) | 342 |
| MvnI | CGCG | 1 cut(s) | 1452 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 204, 1428, 1803 |
| NarI | GGCGCC | 1 cut(s) | 345 |
| NciI | CCSGG | 1 cut(s) | 15 |
| NcoI | CCATGG | 3 cut(s) | 35, 362, 977 |
| NdeI | CATATG | 1 cut(s) | 1829 |
| NdeII | GATC | 3 cut(s) | 192, 234, 597 |
| NlaIV | GGNNCC | 7 cut(s) | 75, 346, 379, 409, 599, 983, 1594 |
| NmeAIII | GCCGAG | 1 cut(s) | 562 |
| NmuCI | GTSAC | 3 cut(s) | 106, 499, 1082 |
| NsbI | TGCGCA | 1 cut(s) | 1052 |
| NspI | RCATGY | 3 cut(s) | 97, 993, 1766 |
| OliI | CACNNNNGTG | 2 cut(s) | 518, 1391 |
| PaeI | GCATGC | 2 cut(s) | 97, 1766 |
| PagI | TCATGA | 1 cut(s) | 645 |
| PciSI | GCTCTTC | 2 cut(s) | 1563, 1848 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 408, 1449 |
| PfeI | GAWTC | 2 cut(s) | 869, 1495 |
| Pfl23II | CGTACG | 1 cut(s) | 102 |
| PflFI | GACNNNGTC | 1 cut(s) | 293 |
| PkrI | GCNGC | 4 cut(s) | 1316, 1324, 1796, 1840 |
| PleI | GAGTC | 5 cut(s) | 48, 827, 1159, 1224, 1694 |
| PluTI | GGCGCC | 1 cut(s) | 348 |
| PpsI | GAGTC | 5 cut(s) | 48, 827, 1159, 1224, 1694 |
| Ppu21I | YACGTR | 2 cut(s) | 106, 1270 |
| Psp124BI | GAGCTC | 2 cut(s) | 22, 445 |
| Psp6I | CCWGG | 1 cut(s) | 340 |
| PspFI | CCCAGC | 1 cut(s) | 1717 |
| PspGI | CCWGG | 1 cut(s) | 340 |
| PspLI | CGTACG | 1 cut(s) | 102 |
| PspN4I | GGNNCC | 7 cut(s) | 75, 346, 379, 409, 599, 983, 1594 |
| PspPI | GGNCC | 7 cut(s) | 12, 73, 268, 378, 407, 768, 1002 |
| PsuI | RGATCY | 1 cut(s) | 597 |
| PsyI | GACNNNGTC | 1 cut(s) | 293 |
| RsaI | GTAC | 5 cut(s) | 62, 104, 983, 1268, 1503 |
| RsaNI | GTAC | 5 cut(s) | 61, 103, 982, 1267, 1502 |
| RseI | CAYNNNNRTG | 4 cut(s) | 386, 518, 572, 1391 |
| SacI | GAGCTC | 2 cut(s) | 22, 445 |
| SapI | GCTCTTC | 2 cut(s) | 1563, 1848 |
| SatI | GCNGC | 4 cut(s) | 1315, 1323, 1795, 1839 |
| Sau3AI | GATC | 3 cut(s) | 192, 234, 597 |
| Sau96I | GGNCC | 7 cut(s) | 12, 73, 268, 378, 407, 768, 1002 |
| SchI | GAGTC | 5 cut(s) | 49, 827, 1160, 1225, 1694 |
| ScrFI | CCNGG | 2 cut(s) | 15, 342 |
| SduI | GDGCHC | 5 cut(s) | 22, 445, 510, 528, 543 |
| SfaNI | GCATC | 1 cut(s) | 150 |
| SfoI | GGCGCC | 1 cut(s) | 346 |
| SinI | GGWCC | 3 cut(s) | 268, 407, 768 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 386, 518, 572, 1391 |
| SmlI | CTYRAG | 2 cut(s) | 713, 1769 |
| SmoI | CTYRAG | 2 cut(s) | 713, 1769 |
| SpeI | ACTAGT | 1 cut(s) | 1300 |
| SphI | GCATGC | 2 cut(s) | 97, 1766 |
| Sse9I | AATT | 2 cut(s) | 823, 1365 |
| SsiI | CCGC | 3 cut(s) | 1622, 1636, 1794 |
| SspDI | GGCGCC | 1 cut(s) | 344 |
| SspI | AATATT | 1 cut(s) | 1345 |
| SspMI | CTAG | 2 cut(s) | 371, 1301 |
| SstI | GAGCTC | 2 cut(s) | 22, 445 |
| StyD4I | CCNGG | 2 cut(s) | 13, 340 |
| StyI | CCWWGG | 5 cut(s) | 35, 46, 362, 931, 977 |
| TaaI | ACNGT | 5 cut(s) | 1076, 1093, 1330, 1506, 1646 |
| TaiI | ACGT | 5 cut(s) | 108, 518, 882, 1272, 1812 |
| TaqI | TCGA | 5 cut(s) | 8, 414, 672, 970, 1698 |
| TaqII | GACCGA | 2 cut(s) | 285, 845 |
| TasI | AATT | 2 cut(s) | 823, 1365 |
| TauI | GCSGC | 1 cut(s) | 1797 |
| TfiI | GAWTC | 2 cut(s) | 869, 1495 |
| TseFI | GTSAC | 3 cut(s) | 106, 499, 1082 |
| TseI | GCWGC | 3 cut(s) | 1314, 1322, 1838 |
| Tsp45I | GTSAC | 3 cut(s) | 106, 499, 1082 |
| TspDTI | ATGAA | 8 cut(s) | 634, 662, 775, 793, 985, 1209, 1244, 1592 |
| TspGWI | ACGGA | 4 cut(s) | 41, 1070, 1376, 1805 |
| Tth111I | GACNNNGTC | 1 cut(s) | 293 |
| VneI | GTGCAC | 1 cut(s) | 524 |
| VpaK11BI | GGWCC | 3 cut(s) | 268, 407, 768 |
| XceI | RCATGY | 3 cut(s) | 97, 993, 1766 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 517, 929 |
| XmiI | GTMKAC | 1 cut(s) | 1508 |
| XspI | CTAG | 2 cut(s) | 371, 1301 |
| ZraI | GACGTC | 1 cut(s) | 880 |