RchiOBHm_Chr1g0343691

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000300
Physical Location & Seq
Reverse (-)
35887852 .. 35892166
4315 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57020

Sequence Viewer

Length: 1899 bp
ATGGCTTCCACACCTGGAATTCTTACTAACTGGCCATGGACACCTCTTGGGAGCTTTAAGTACGTGGTGTTAGCTCCTTGGGTGATTCACAGCACATACTCATATATGTTTGGAGAAGAAGGCACAGATCTATTTTACATCCTCGTCTTTCCATTTCTGTTGTGGAGGATGCTCCACAACCAGATATGGATCTCACTTTCTCGCTATCAAACTGCCAAAGGCAAAGGAACAATCGTGGACAAGGGCCTCGAATTTGAACAAGTCGACAGAGAAAGAGATTGGGATGATCAAATCATATTCAATGGAATCCTATTTTACTTAGCCAGGTGCGTCCTGCCTATGACTCAAACTGACCTACCAATTTGGAGGGGAAATGGGTTGGTCATGACGATGCTGCTTCATGCTGGTCCGGTCGAGTACCTATACTACTGGTTACACAGAGCACTTCACCACCATTTCCTCTACTCCCGCTACCATTCTCATCACCATTCCTCCATTGTCACTGAGCCAATTACTTCTGTCATTCACCCATTTGCAGAGCACATAACATATTTCATGCTCTTCGCAATACCAATTGTGGCAACTCTGTTGATCGGAACAGCTTCCATCTCATCCTATTTTGTTTATATTACTTATATCGACTTCATGAACAACATGGGGCACTGCAATTTCGAGTTCATTCCGAACTGGATCTTCTCTCTTTTTCCTCCTATTAAGTATCTAATATATACTCCCTCGTACCACTCCTTGCATCACACACAATTTCGAACCAATTACTCCCTCTTCATGCCAATCTATGACTATATATATGGTACCATGGACAAGTCTAGTGATACTCTACACGAATCTTCACTCAGGAAAGAGGAATCACCTGATGTGGTTCATCTAACCCATTTAACAACAACTGAATCCATCTACCGACTCCCACTGGCATTTGCTTCCTTGGCCTCTAATCCACACACTTCAACATGGTACTTATGGTTGTTGTGGCCTGTGACATTATGGTCCTTGATGCTAACTTGGATATTTGGCCGTTCATTTGTTATTGAGAGGCAGCGTTTTGATAAACACAGACTACAAACTTGGGTTATTCCAAAATACAGTTTGCAGTACTTGCTCTCATGGCAAACTGAAGCTATAAACAGAGCGATTGAGGATGCTATACTTGAAGCTGAGCAAATGGGTGTCAAAGTTATAAATTTAGGTCTCTTTAATCAGGGTGAGGGGCTCAATAATTATGGTGGTATATATGTCCAGAGGAATCCACGGCTGAAAGTCAAGGTGGTGGATGGGAGTAGCTTGGCTGTGGCCGTCATCCTTAACAGCATTCCGAAAGGGACAACCCAGGTTCTCCTTACAGGCAACCTCACAAAGGTTGCTTATGCCCTTGCCTTTTCTTTGTGCCAAAGAGATATCCAGGTAGTTACATTACAGCAGGCTGAGTATTTGAAGCTCACCAAATCATTAAATGCTACAGAGGGTAAGGTGGTTCATGACAAAAGCTATGCTCAAAAGATTTGGTTGGTGGGAGATGGGTTGAGTAAACAAGAACAGTTGAGTGCACCAAAAGGAACAATTTTTGTTCCCTTCTCTCAATTTCCACCAAAACAGTTGCGCAGAGACTGCCTCTACCACTACACACCAGCAATGAAGATCCCCAAGTCTCTTGAGAATGTTTACTCTTGTGAGAACTGGTTGCCAAGGAGGGTGATGAGTGCGTCGCGTATAGCAGGAATAGTGCATGCATTGGAAGGTTGGAACGAGCACGAGTGTGGTCACACCATGTCCAGCATTGACAAAGTTTGGCAAGCAACTCTTCGACATGGCTTTCAGCCTCTAGTCTCCAAAGAGGCAAAGATTGATAAGTCACCTCTTCAAGAAAGCATTACCCAATTCTGA

Protein Analysis

632

Amino Acids

73.08

Weight (kDa)

8.29

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 136 - 272 7.6e-22 Fatty acid hydroxylase
CER1-like_C PF12076 450 - 612 1.8e-73 CER1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1196
AasI GACNNNNNNGTC 1 cut(s) 1003
Acc16I TGCGCA 1 cut(s) 1616
Acc65I GGTACC 1 cut(s) 812
AccB1I GGYRCC 1 cut(s) 812
AccI GTMKAC 1 cut(s) 264
AccII CGCG 1 cut(s) 1723
AciI CCGC 1 cut(s) 469
AclWI GGATC 3 cut(s) 197, 698, 1648
AcoI YGGCCR 3 cut(s) 32, 1030, 1308
AcsI RAATTY 3 cut(s) 18, 251, 1198
AcuI CTGAAG 1 cut(s) 1152
AfaI GTAC 6 cut(s) 62, 419, 740, 814, 974, 1112
AfiI CCNNNNNNNGG 1 cut(s) 1372
AgsI TTSAA 6 cut(s) 257, 301, 966, 1169, 1450, 1877
AjnI CCWGG 4 cut(s) 13, 323, 1344, 1416
AleI CACNNNNGTG 1 cut(s) 1770
AluBI AGCT 8 cut(s) 54, 74, 602, 1136, 1172, 1299, 1453, 1503
AluI AGCT 8 cut(s) 54, 74, 602, 1136, 1172, 1299, 1453, 1503
Alw21I GWGCWC 4 cut(s) 445, 543, 1564, 1767
Alw26I GTCTC 4 cut(s) 1211, 1614, 1668, 1846
Alw44I GTGCAC 1 cut(s) 1560
AlwI GGATC 3 cut(s) 197, 698, 1648
AlwNI CAGNNNCTG 1 cut(s) 1623
AoxI GGCC 6 cut(s) 32, 244, 945, 989, 1030, 1308
ApaLI GTGCAC 1 cut(s) 1560
ApeKI GCWGC 2 cut(s) 394, 1054
ApoI RAATTY 3 cut(s) 18, 251, 1198
Asp700I GAANNNNTTC 1 cut(s) 601
Asp718I GGTACC 1 cut(s) 812
AspLEI GCGC 1 cut(s) 1617
AspS9I GGNCC 3 cut(s) 244, 407, 1005
AsuHPI GGTGA 9 cut(s) 94, 440, 476, 518, 861, 1232, 1447, 1720, 1860
AsuII TTCGAA 1 cut(s) 766
AvaII GGWCC 2 cut(s) 407, 1005
BaeGI GKGCMC 2 cut(s) 663, 1564
BalI TGGCCA 1 cut(s) 34
BanI GGYRCC 1 cut(s) 812
BanII GRGCYC 1 cut(s) 1230
BauI CACGAG 1 cut(s) 1766
Bbv12I GWGCWC 4 cut(s) 445, 543, 1564, 1767
BbvI GCAGC 2 cut(s) 381, 1066
BccI CCATC 4 cut(s) 614, 920, 1283, 1526
BceAI ACGGC 3 cut(s) 1017, 1283, 1295
BciT130I CCWGG 4 cut(s) 15, 325, 1346, 1418
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 4 cut(s) 1211, 1614, 1668, 1846
BfaI CTAG 2 cut(s) 828, 1838
BfmI CTRYAG 1 cut(s) 1473
BglII AGATCT 1 cut(s) 127
BisI GCNGC 2 cut(s) 395, 1055
BlpI GCTNAGC 1 cut(s) 1173
BlsI GCNGC 2 cut(s) 396, 1056
BmcAI AGTACT 1 cut(s) 1112
Bme1390I CCNGG 4 cut(s) 15, 325, 1346, 1418
Bme18I GGWCC 2 cut(s) 407, 1005
BmgT120I GGNCC 3 cut(s) 244, 407, 1005
BmiI GGNNCC 1 cut(s) 814
BmrFI CCNGG 4 cut(s) 15, 325, 1346, 1418
BmsI GCATC 5 cut(s) 159, 381, 760, 1002, 1147
BplI GAGNNNNNCTC 2 cut(s) 1611, 1643
Bpu1102I GCTNAGC 1 cut(s) 1173
Bpu14I TTCGAA 1 cut(s) 766
BpuEI CTTGAG 1 cut(s) 1688
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 188
BsaI GGTCTC 1 cut(s) 1211
BsaJI CCNNGG 7 cut(s) 35, 77, 816, 942, 1265, 1344, 1700
BsaWI WCCGGW 1 cut(s) 409
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 1372
Bse1I ACTGG 5 cut(s) 35, 434, 692, 933, 1697
Bse3DI GCAATG 1 cut(s) 1653
Bse8I GATNNNNATC 1 cut(s) 188
BseBI CCWGG 4 cut(s) 15, 325, 1346, 1418
BseDI CCNNGG 7 cut(s) 35, 77, 816, 942, 1265, 1344, 1700
BseGI GGATG 7 cut(s) 138, 174, 289, 611, 1162, 1294, 1314
BseJI GATNNNNATC 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 1372
BseMI GCAATG 1 cut(s) 1653
BseMII CTCAG 4 cut(s) 495, 868, 1164, 1431
BseNI ACTGG 5 cut(s) 35, 434, 692, 933, 1697
BseSI GKGCMC 2 cut(s) 663, 1564
BseXI GCAGC 2 cut(s) 381, 1066
Bsh1236I CGCG 1 cut(s) 1723
Bsh1285I CGRYCG 1 cut(s) 414
BshFI GGCC 6 cut(s) 34, 246, 947, 991, 1032, 1310
BshNI GGYRCC 1 cut(s) 812
BsiEI CGRYCG 1 cut(s) 414
BsiHKAI GWGCWC 4 cut(s) 445, 543, 1564, 1767
BsiSI CCGG 1 cut(s) 410
BslFI GGGAC 1 cut(s) 1351
BslI CCNNNNNNNGG 1 cut(s) 1372
BsmAI GTCTC 4 cut(s) 1211, 1614, 1668, 1846
BsmFI GGGAC 1 cut(s) 1351
BsmI GAATGC 1 cut(s) 1326
BsnI GGCC 6 cut(s) 34, 246, 947, 991, 1032, 1310
Bso31I GGTCTC 1 cut(s) 1211
Bsp119I TTCGAA 1 cut(s) 766
Bsp1286I GDGCHC 6 cut(s) 445, 543, 663, 1230, 1564, 1767
Bsp143I GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
Bsp1720I GCTNAGC 1 cut(s) 1173
Bsp19I CCATGG 2 cut(s) 35, 816
BspACI CCGC 1 cut(s) 469
BspANI GGCC 6 cut(s) 34, 246, 947, 991, 1032, 1310
BspCNI CTCAG 4 cut(s) 496, 867, 1165, 1432
BspFNI CGCG 1 cut(s) 1723
BspHI TCATGA 3 cut(s) 384, 645, 1492
BspLI GGNNCC 1 cut(s) 814
BspPI GGATC 3 cut(s) 197, 698, 1648
BspQI GCTCTTC 1 cut(s) 566
BspT104I TTCGAA 1 cut(s) 766
BspT107I GGYRCC 1 cut(s) 812
BspTNI GGTCTC 1 cut(s) 1211
BsrDI GCAATG 1 cut(s) 1653
BsrI ACTGG 5 cut(s) 35, 434, 692, 933, 1697
BssECI CCNNGG 7 cut(s) 35, 77, 816, 942, 1265, 1344, 1700
BssMI GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
BssSI CACGAG 1 cut(s) 1766
BssT1I CCWWGG 5 cut(s) 35, 77, 816, 942, 1700
Bst2BI CACGAG 1 cut(s) 1766
Bst2UI CCWGG 4 cut(s) 15, 325, 1346, 1418
Bst4CI ACNGT 3 cut(s) 1103, 1554, 1611
Bst6I CTCTTC 4 cut(s) 566, 788, 1821, 1878
BstAPI GCANNNNNTGC 2 cut(s) 1114, 1623
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 766
BstC8I GCNNGC 3 cut(s) 1437, 1743, 1809
BstDEI CTNAG 5 cut(s) 319, 504, 854, 1173, 1440
BstDSI CCRYGG 3 cut(s) 35, 816, 1265
BstENI CCTNNNNNAGG 1 cut(s) 1370
BstF5I GGATG 7 cut(s) 138, 174, 289, 611, 1162, 1294, 1314
BstFNI CGCG 1 cut(s) 1723
BstHHI GCGC 1 cut(s) 1617
BstKTI GATC 6 cut(s) 130, 192, 289, 594, 693, 1656
BstMAI GTCTC 4 cut(s) 1211, 1614, 1668, 1846
BstMBI GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
BstMCI CGRYCG 1 cut(s) 414
BstMWI GCNNNNNNNGC 4 cut(s) 944, 1114, 1123, 1623
BstNI CCWGG 4 cut(s) 15, 325, 1346, 1418
BstNSI RCATGY 1 cut(s) 1745
BstSCI CCNGG 4 cut(s) 13, 323, 1344, 1416
BstSFI CTRYAG 1 cut(s) 1473
BstSLI GKGCMC 2 cut(s) 663, 1564
BstUI CGCG 1 cut(s) 1723
BstV1I GCAGC 2 cut(s) 381, 1066
BstX2I RGATCY 4 cut(s) 127, 189, 690, 1653
BstYI RGATCY 4 cut(s) 127, 189, 690, 1653
BsuRI GGCC 6 cut(s) 34, 246, 947, 991, 1032, 1310
BtgI CCRYGG 3 cut(s) 35, 816, 1265
BtsCI GGATG 7 cut(s) 138, 174, 289, 611, 1162, 1294, 1314
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 3 cut(s) 501, 661, 926
Cac8I GCNNGC 3 cut(s) 1437, 1743, 1809
CaiI CAGNNNCTG 1 cut(s) 1623
CciI TCATGA 3 cut(s) 384, 645, 1492
CfoI GCGC 1 cut(s) 1617
Cfr13I GGNCC 3 cut(s) 244, 407, 1005
CseI GACGC 2 cut(s) 319, 1707
Csp6I GTAC 6 cut(s) 61, 418, 739, 813, 973, 1111
CviQI GTAC 6 cut(s) 61, 418, 739, 813, 973, 1111
DdeI CTNAG 5 cut(s) 319, 504, 854, 1173, 1440
DpnI GATC 6 cut(s) 129, 191, 288, 593, 692, 1655
DpnII GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
DrdI GACNNNNNNGTC 1 cut(s) 1003
DseDI GACNNNNNNGTC 1 cut(s) 1003
EaeI YGGCCR 3 cut(s) 32, 1030, 1308
Eam1104I CTCTTC 4 cut(s) 566, 788, 1821, 1878
EarI CTCTTC 4 cut(s) 566, 788, 1821, 1878
Eco130I CCWWGG 5 cut(s) 35, 77, 816, 942, 1700
Eco24I GRGCYC 1 cut(s) 1230
Eco31I GGTCTC 1 cut(s) 1211
Eco32I GATATC 1 cut(s) 1414
Eco47I GGWCC 2 cut(s) 407, 1005
Eco57I CTGAAG 1 cut(s) 1152
EcoNI CCTNNNNNAGG 1 cut(s) 1370
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 4 cut(s) 13, 323, 1344, 1416
EcoRV GATATC 1 cut(s) 1414
EcoT14I CCWWGG 5 cut(s) 35, 77, 816, 942, 1700
EcoT22I ATGCAT 1 cut(s) 1747
EcoT38I GRGCYC 1 cut(s) 1230
ErhI CCWWGG 5 cut(s) 35, 77, 816, 942, 1700
FalI AAGNNNNNCTT 2 cut(s) 1800, 1832
FaqI GGGAC 1 cut(s) 1351
FauI CCCGC 1 cut(s) 476
FbaI TGATCA 1 cut(s) 286
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 395, 1055
FokI GGATG 7 cut(s) 125, 181, 296, 598, 1169, 1301, 1301
FriOI GRGCYC 1 cut(s) 1230
Fsp4HI GCNGC 2 cut(s) 395, 1055
FspBI CTAG 2 cut(s) 828, 1838
FspI TGCGCA 1 cut(s) 1616
GlaI GCGC 1 cut(s) 1616
GluI GCNGC 2 cut(s) 395, 1055
HaeIII GGCC 6 cut(s) 34, 246, 947, 991, 1032, 1310
HapII CCGG 1 cut(s) 410
HgaI GACGC 2 cut(s) 319, 1707
HhaI GCGC 1 cut(s) 1617
Hin6I GCGC 1 cut(s) 1615
HinP1I GCGC 1 cut(s) 1615
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 8 cut(s) 85, 306, 343, 845, 866, 908, 921, 1261
HpaII CCGG 1 cut(s) 410
HphI GGTGA 9 cut(s) 94, 440, 476, 518, 861, 1232, 1447, 1720, 1860
Hpy166II GTNNAC 5 cut(s) 238, 265, 1544, 1562, 1678
Hpy188I TCNGA 4 cut(s) 596, 684, 1332, 1898
Hpy188III TCNNGA 7 cut(s) 385, 646, 856, 1255, 1493, 1667, 1877
Hpy8I GTNNAC 5 cut(s) 238, 265, 1544, 1562, 1678
Hpy99I CGWCG 1 cut(s) 1723
HpyAV CCTTC 3 cut(s) 113, 1597, 1745
HpyCH4III ACNGT 3 cut(s) 1103, 1554, 1611
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 7 cut(s) 536, 666, 751, 1108, 1562, 1741, 1745
HpyF10VI GCNNNNNNNGC 4 cut(s) 944, 1114, 1123, 1623
HpyF3I CTNAG 5 cut(s) 319, 504, 854, 1173, 1440
HpySE526I ACGT 1 cut(s) 63
HspAI GCGC 1 cut(s) 1615
KpnI GGTACC 1 cut(s) 816
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
LguI GCTCTTC 1 cut(s) 566
LmnI GCTCC 3 cut(s) 51, 79, 177
Lsp1109I GCAGC 2 cut(s) 381, 1066
LweI GCATC 5 cut(s) 159, 381, 760, 1002, 1147
MaeI CTAG 2 cut(s) 828, 1838
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 6 cut(s) 432, 499, 994, 1423, 1775, 1866
MalI GATC 6 cut(s) 129, 191, 288, 593, 692, 1655
MboI GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
MboII GAAGA 8 cut(s) 128, 553, 685, 775, 840, 1663, 1808, 1865
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 4 cut(s) 127, 189, 690, 1653
MhlI GDGCHC 6 cut(s) 445, 543, 663, 1230, 1564, 1767
MlsI TGGCCA 1 cut(s) 34
MluNI TGGCCA 1 cut(s) 34
MlyI GAGTC 2 cut(s) 337, 915
MmeI TCCRAC 1 cut(s) 1736
Mox20I TGGCCA 1 cut(s) 34
Mph1103I ATGCAT 1 cut(s) 1747
MroXI GAANNNNTTC 1 cut(s) 601
MscI TGGCCA 1 cut(s) 34
MseI TTAA 6 cut(s) 57, 714, 896, 1212, 1320, 1466
MslI CAYNNNNRTG 2 cut(s) 389, 1770
Msp20I TGGCCA 1 cut(s) 34
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 4 cut(s) 15, 325, 1346, 1418
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 1326
MvaI CCWGG 4 cut(s) 15, 325, 1346, 1418
MvnI CGCG 1 cut(s) 1723
MwoI GCNNNNNNNGC 4 cut(s) 944, 1114, 1123, 1623
NcoI CCATGG 2 cut(s) 35, 816
NdeII GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
NlaIV GGNNCC 1 cut(s) 814
NmuCI GTSAC 4 cut(s) 499, 994, 1775, 1866
NsbI TGCGCA 1 cut(s) 1616
NsiI ATGCAT 1 cut(s) 1747
NspI RCATGY 1 cut(s) 1745
NspV TTCGAA 1 cut(s) 766
OliI CACNNNNGTG 1 cut(s) 1770
PaeI GCATGC 1 cut(s) 1745
PagI TCATGA 3 cut(s) 384, 645, 1492
PciSI GCTCTTC 1 cut(s) 566
PctI GAATGC 1 cut(s) 1326
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 6 cut(s) 85, 306, 845, 866, 908, 1261
PkrI GCNGC 2 cut(s) 396, 1056
PleI GAGTC 2 cut(s) 337, 915
PpsI GAGTC 2 cut(s) 337, 915
Ppu21I YACGTR 1 cut(s) 64
PsiI TTATAA 1 cut(s) 1196
Psp6I CCWGG 4 cut(s) 13, 323, 1344, 1416
PspGI CCWGG 4 cut(s) 13, 323, 1344, 1416
PspN4I GGNNCC 1 cut(s) 814
PspPI GGNCC 3 cut(s) 244, 407, 1005
PstNI CAGNNNCTG 1 cut(s) 1623
PsuI RGATCY 4 cut(s) 127, 189, 690, 1653
RsaI GTAC 6 cut(s) 62, 419, 740, 814, 974, 1112
RsaNI GTAC 6 cut(s) 61, 418, 739, 813, 973, 1111
RseI CAYNNNNRTG 2 cut(s) 389, 1770
SalI GTCGAC 1 cut(s) 263
SapI GCTCTTC 1 cut(s) 566
SaqAI TTAA 6 cut(s) 57, 714, 896, 1212, 1320, 1466
SatI GCNGC 2 cut(s) 395, 1055
Sau3AI GATC 6 cut(s) 127, 189, 286, 591, 690, 1653
Sau96I GGNCC 3 cut(s) 244, 407, 1005
ScaI AGTACT 1 cut(s) 1112
SchI GAGTC 2 cut(s) 337, 915
ScrFI CCNGG 4 cut(s) 15, 325, 1346, 1418
SduI GDGCHC 6 cut(s) 445, 543, 663, 1230, 1564, 1767
SfaNI GCATC 5 cut(s) 159, 381, 760, 1002, 1147
SfcI CTRYAG 1 cut(s) 1473
SfuI TTCGAA 1 cut(s) 766
SinI GGWCC 2 cut(s) 407, 1005
SmiMI CAYNNNNRTG 2 cut(s) 389, 1770
SmlI CTYRAG 1 cut(s) 1667
SmoI CTYRAG 1 cut(s) 1667
SphI GCATGC 1 cut(s) 1745
SsiI CCGC 1 cut(s) 469
SspMI CTAG 2 cut(s) 828, 1838
StyD4I CCNGG 4 cut(s) 13, 323, 1344, 1416
StyI CCWWGG 5 cut(s) 35, 77, 816, 942, 1700
TaaI ACNGT 3 cut(s) 1103, 1554, 1611
TaiI ACGT 1 cut(s) 66
TaqI TCGA 7 cut(s) 249, 264, 414, 639, 672, 766, 1819
TatI WGTACW 1 cut(s) 1110
TfiI GAWTC 6 cut(s) 85, 306, 845, 866, 908, 1261
Tru1I TTAA 6 cut(s) 57, 714, 896, 1212, 1320, 1466
Tru9I TTAA 6 cut(s) 57, 714, 896, 1212, 1320, 1466
TscAI CASTG 3 cut(s) 508, 668, 933
TseFI GTSAC 4 cut(s) 499, 994, 1775, 1866
TseI GCWGC 2 cut(s) 394, 1054
Tsp45I GTSAC 4 cut(s) 499, 994, 1775, 1866
TspRI CASTG 3 cut(s) 508, 668, 933
VneI GTGCAC 1 cut(s) 1560
VpaK11BI GGWCC 2 cut(s) 407, 1005
XagI CCTNNNNNAGG 1 cut(s) 1370
XapI RAATTY 3 cut(s) 18, 251, 1198
XceI RCATGY 1 cut(s) 1745
XcmI CCANNNNNNNNNTGG 1 cut(s) 159
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 2 cut(s) 828, 1838
ZrmI AGTACT 1 cut(s) 1112
Zsp2I ATGCAT 1 cut(s) 1747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.