Belongs to the sterol desaturase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 345 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1663 |
| Acc16I | TGCGCA | 1 cut(s) | 1619 |
| Acc36I | ACCTGC | 1 cut(s) | 345 |
| AccB1I | GGYRCC | 2 cut(s) | 333, 812 |
| AccI | GTMKAC | 2 cut(s) | 240, 267 |
| AccIII | TCCGGA | 1 cut(s) | 765 |
| AciI | CCGC | 2 cut(s) | 216, 536 |
| AclWI | GGATC | 3 cut(s) | 200, 242, 619 |
| AcoI | YGGCCR | 4 cut(s) | 32, 992, 1033, 1851 |
| AcsI | RAATTY | 2 cut(s) | 254, 1599 |
| AcyI | GRCGYC | 1 cut(s) | 41 |
| AdeI | CACNNNGTG | 1 cut(s) | 1005 |
| AfaI | GTAC | 4 cut(s) | 62, 719, 728, 1115 |
| AfeI | AGCGCT | 1 cut(s) | 1061 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 343, 535, 1375 |
| AflII | CTTAAG | 1 cut(s) | 713 |
| AflIII | ACRYGT | 1 cut(s) | 963 |
| AgsI | TTSAA | 6 cut(s) | 304, 1172, 1216, 1453, 1471, 1561 |
| AjiI | CACGTC | 1 cut(s) | 966 |
| AjnI | CCWGG | 3 cut(s) | 13, 340, 1419 |
| AleI | CACNNNNGTG | 1 cut(s) | 1773 |
| Alw21I | GWGCWC | 2 cut(s) | 445, 678 |
| Alw26I | GTCTC | 1 cut(s) | 1214 |
| AlwI | GGATC | 3 cut(s) | 200, 242, 619 |
| Ama87I | CYCGRG | 1 cut(s) | 1670 |
| Aor13HI | TCCGGA | 1 cut(s) | 765 |
| Aor51HI | AGCGCT | 1 cut(s) | 1061 |
| AoxI | GGCC | 7 cut(s) | 32, 229, 948, 992, 1033, 1246, 1851 |
| ApeKI | GCWGC | 5 cut(s) | 54, 327, 1015, 1057, 1511 |
| ApoI | RAATTY | 2 cut(s) | 254, 1599 |
| AspA2I | CCTAGG | 1 cut(s) | 322 |
| AspLEI | GCGC | 3 cut(s) | 1062, 1620, 1728 |
| AspS9I | GGNCC | 3 cut(s) | 119, 407, 768 |
| AsuHPI | GGTGA | 6 cut(s) | 440, 493, 518, 589, 743, 1723 |
| AsuII | TTCGAA | 1 cut(s) | 258 |
| AvaI | CYCGRG | 1 cut(s) | 1670 |
| AvaII | GGWCC | 3 cut(s) | 119, 407, 768 |
| AvrII | CCTAGG | 1 cut(s) | 322 |
| BaeGI | GKGCMC | 2 cut(s) | 663, 1770 |
| BalI | TGGCCA | 2 cut(s) | 34, 994 |
| BanI | GGYRCC | 2 cut(s) | 333, 812 |
| BanII | GRGCYC | 3 cut(s) | 349, 445, 678 |
| BauI | CACGAG | 1 cut(s) | 1769 |
| BbsI | GAAGAC | 1 cut(s) | 1661 |
| Bbv12I | GWGCWC | 2 cut(s) | 445, 678 |
| BbvCI | CCTCAGC | 1 cut(s) | 1176 |
| BbvI | GCAGC | 5 cut(s) | 41, 339, 1002, 1069, 1498 |
| BccI | CCATC | 5 cut(s) | 368, 800, 903, 923, 1286 |
| BceAI | ACGGC | 5 cut(s) | 106, 826, 1048, 1162, 1866 |
| BciT130I | CCWGG | 3 cut(s) | 15, 342, 1421 |
| BcoDI | GTCTC | 1 cut(s) | 1214 |
| BfaI | CTAG | 1 cut(s) | 323 |
| BfmI | CTRYAG | 1 cut(s) | 930 |
| BfoI | RGCGCY | 1 cut(s) | 1063 |
| BfrI | CTTAAG | 1 cut(s) | 713 |
| BfuAI | ACCTGC | 1 cut(s) | 345 |
| BglII | AGATCT | 2 cut(s) | 130, 1518 |
| BisI | GCNGC | 5 cut(s) | 55, 328, 1016, 1058, 1512 |
| BlnI | CCTAGG | 1 cut(s) | 322 |
| BlpI | GCTNAGC | 1 cut(s) | 1303 |
| BlsI | GCNGC | 5 cut(s) | 56, 329, 1017, 1059, 1513 |
| BmcAI | AGTACT | 1 cut(s) | 1115 |
| Bme1390I | CCNGG | 3 cut(s) | 15, 342, 1421 |
| Bme18I | GGWCC | 3 cut(s) | 119, 407, 768 |
| BmeT110I | CYCGRG | 1 cut(s) | 1670 |
| BmgBI | CACGTC | 1 cut(s) | 966 |
| BmgT120I | GGNCC | 3 cut(s) | 119, 407, 768 |
| BmiI | GGNNCC | 3 cut(s) | 75, 335, 814 |
| BmrFI | CCNGG | 3 cut(s) | 15, 342, 1421 |
| BmrI | ACTGGG | 1 cut(s) | 292 |
| BmsI | GCATC | 2 cut(s) | 162, 895 |
| BmuI | ACTGGG | 1 cut(s) | 292 |
| BpiI | GAAGAC | 1 cut(s) | 1661 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1353, 1385 |
| BpmI | CTGGAG | 1 cut(s) | 1629 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1176 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1303 |
| Bpu14I | TTCGAA | 1 cut(s) | 258 |
| BsaAI | YACGTR | 1 cut(s) | 64 |
| BsaBI | GATNNNNATC | 1 cut(s) | 129 |
| BsaHI | GRCGYC | 1 cut(s) | 41 |
| BsaI | GGTCTC | 1 cut(s) | 1214 |
| BsaJI | CCNNGG | 8 cut(s) | 35, 77, 115, 322, 341, 816, 945, 1848 |
| BsaWI | WCCGGW | 2 cut(s) | 409, 765 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 476, 506 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 343, 535, 1375 |
| Bse118I | RCCGGY | 1 cut(s) | 404 |
| Bse1I | ACTGG | 8 cut(s) | 35, 184, 287, 434, 692, 995, 1438, 1700 |
| Bse3DI | GCAATG | 1 cut(s) | 1728 |
| Bse8I | GATNNNNATC | 1 cut(s) | 129 |
| BseAI | TCCGGA | 1 cut(s) | 765 |
| BseBI | CCWGG | 3 cut(s) | 15, 342, 1421 |
| BseDI | CCNNGG | 8 cut(s) | 35, 77, 115, 322, 341, 816, 945, 1848 |
| BseGI | GGATG | 5 cut(s) | 177, 292, 555, 1297, 1317 |
| BseJI | GATNNNNATC | 1 cut(s) | 129 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 343, 535, 1375 |
| BseMI | GCAATG | 1 cut(s) | 1728 |
| BseMII | CTCAG | 3 cut(s) | 1167, 1470, 1874 |
| BseNI | ACTGG | 8 cut(s) | 35, 184, 287, 434, 692, 995, 1438, 1700 |
| BseRI | GAGGAG | 3 cut(s) | 699, 878, 1241 |
| BseSI | GKGCMC | 2 cut(s) | 663, 1770 |
| BseX3I | CGGCCG | 1 cut(s) | 1033 |
| BseXI | GCAGC | 5 cut(s) | 41, 339, 1002, 1069, 1498 |
| BsgI | GTGCAG | 1 cut(s) | 1624 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1036 |
| BshFI | GGCC | 7 cut(s) | 34, 231, 950, 994, 1035, 1248, 1853 |
| BshNI | GGYRCC | 2 cut(s) | 333, 812 |
| BsiEI | CGRYCG | 1 cut(s) | 1036 |
| BsiHKAI | GWGCWC | 2 cut(s) | 445, 678 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1670 |
| BsiSI | CCGG | 5 cut(s) | 232, 405, 410, 766, 1734 |
| BslFI | GGGAC | 3 cut(s) | 261, 610, 1354 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 343, 535, 1375 |
| BsmAI | GTCTC | 1 cut(s) | 1214 |
| BsmFI | GGGAC | 3 cut(s) | 261, 610, 1354 |
| BsmI | GAATGC | 3 cut(s) | 18, 1329, 1567 |
| BsnI | GGCC | 7 cut(s) | 34, 231, 950, 994, 1035, 1248, 1853 |
| Bso31I | GGTCTC | 1 cut(s) | 1214 |
| BsoBI | CYCGRG | 1 cut(s) | 1670 |
| Bsp119I | TTCGAA | 1 cut(s) | 258 |
| Bsp1286I | GDGCHC | 5 cut(s) | 349, 445, 663, 678, 1770 |
| Bsp13I | TCCGGA | 1 cut(s) | 765 |
| Bsp1407I | TGTACA | 1 cut(s) | 726 |
| Bsp143I | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1303 |
| Bsp19I | CCATGG | 2 cut(s) | 35, 816 |
| BspACI | CCGC | 2 cut(s) | 216, 536 |
| BspANI | GGCC | 7 cut(s) | 34, 231, 950, 994, 1035, 1248, 1853 |
| BspCNI | CTCAG | 3 cut(s) | 1168, 1471, 1873 |
| BspEI | TCCGGA | 1 cut(s) | 765 |
| BspLI | GGNNCC | 3 cut(s) | 75, 335, 814 |
| BspMI | ACCTGC | 1 cut(s) | 345 |
| BspPI | GGATC | 3 cut(s) | 200, 242, 619 |
| BspQI | GCTCTTC | 1 cut(s) | 450 |
| BspT104I | TTCGAA | 1 cut(s) | 258 |
| BspT107I | GGYRCC | 2 cut(s) | 333, 812 |
| BspTI | CTTAAG | 1 cut(s) | 713 |
| BspTNI | GGTCTC | 1 cut(s) | 1214 |
| BsrDI | GCAATG | 1 cut(s) | 1728 |
| BsrFI | RCCGGY | 1 cut(s) | 404 |
| BsrGI | TGTACA | 1 cut(s) | 726 |
| BsrI | ACTGG | 8 cut(s) | 35, 184, 287, 434, 692, 995, 1438, 1700 |
| BssAI | RCCGGY | 1 cut(s) | 404 |
| BssECI | CCNNGG | 8 cut(s) | 35, 77, 115, 322, 341, 816, 945, 1848 |
| BssMI | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| BssNI | GRCGYC | 1 cut(s) | 41 |
| BssSI | CACGAG | 1 cut(s) | 1769 |
| BssT1I | CCWWGG | 6 cut(s) | 35, 77, 115, 322, 816, 945 |
| Bst2BI | CACGAG | 1 cut(s) | 1769 |
| Bst2UI | CCWGG | 3 cut(s) | 15, 342, 1421 |
| Bst4CI | ACNGT | 3 cut(s) | 1005, 1106, 1244 |
| Bst6I | CTCTTC | 5 cut(s) | 450, 566, 753, 788, 832 |
| BstACI | GRCGYC | 1 cut(s) | 41 |
| BstAFI | CTTAAG | 1 cut(s) | 713 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1626 |
| BstAUI | TGTACA | 1 cut(s) | 726 |
| BstBAI | YACGTR | 1 cut(s) | 64 |
| BstBI | TTCGAA | 1 cut(s) | 258 |
| BstC8I | GCNNGC | 3 cut(s) | 332, 1746, 1812 |
| BstDEI | CTNAG | 8 cut(s) | 954, 1176, 1303, 1358, 1374, 1479, 1750, 1860 |
| BstDSI | CCRYGG | 3 cut(s) | 35, 816, 1848 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| BstF5I | GGATG | 5 cut(s) | 177, 292, 555, 1297, 1317 |
| BstH2I | RGCGCY | 1 cut(s) | 1063 |
| BstHHI | GCGC | 3 cut(s) | 1062, 1620, 1728 |
| BstKTI | GATC | 5 cut(s) | 133, 195, 237, 614, 1521 |
| BstMAI | GTCTC | 1 cut(s) | 1214 |
| BstMBI | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| BstMCI | CGRYCG | 1 cut(s) | 1036 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 40, 947, 1626 |
| BstNI | CCWGG | 3 cut(s) | 15, 342, 1421 |
| BstNSI | RCATGY | 1 cut(s) | 1748 |
| BstSCI | CCNGG | 3 cut(s) | 13, 340, 1419 |
| BstSFI | CTRYAG | 1 cut(s) | 930 |
| BstSLI | GKGCMC | 2 cut(s) | 663, 1770 |
| BstV1I | GCAGC | 5 cut(s) | 41, 339, 1002, 1069, 1498 |
| BstV2I | GAAGAC | 1 cut(s) | 1661 |
| BstX2I | RGATCY | 3 cut(s) | 130, 192, 1518 |
| BstYI | RGATCY | 3 cut(s) | 130, 192, 1518 |
| BstZI | CGGCCG | 1 cut(s) | 1033 |
| BsuRI | GGCC | 7 cut(s) | 34, 231, 950, 994, 1035, 1248, 1853 |
| BtgI | CCRYGG | 3 cut(s) | 35, 816, 1848 |
| BtrI | CACGTC | 1 cut(s) | 966 |
| BtsCI | GGATG | 5 cut(s) | 177, 292, 555, 1297, 1317 |
| BtsI | GCAGTG | 2 cut(s) | 661, 1636 |
| BtsIMutI | CAGTG | 4 cut(s) | 661, 1001, 1476, 1636 |
| BveI | ACCTGC | 1 cut(s) | 345 |
| Cac8I | GCNNGC | 3 cut(s) | 332, 1746, 1812 |
| CfoI | GCGC | 3 cut(s) | 1062, 1620, 1728 |
| Cfr10I | RCCGGY | 1 cut(s) | 404 |
| Cfr13I | GGNCC | 3 cut(s) | 119, 407, 768 |
| CpoI | CGGWCCG | 1 cut(s) | 407 |
| CseI | GACGC | 1 cut(s) | 49 |
| Csp6I | GTAC | 4 cut(s) | 61, 718, 727, 1114 |
| CspI | CGGWCCG | 1 cut(s) | 407 |
| CviQI | GTAC | 4 cut(s) | 61, 718, 727, 1114 |
| DdeI | CTNAG | 8 cut(s) | 954, 1176, 1303, 1358, 1374, 1479, 1750, 1860 |
| DpnI | GATC | 5 cut(s) | 132, 194, 236, 613, 1520 |
| DpnII | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| DraIII | CACNNNGTG | 1 cut(s) | 1005 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1663 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1663 |
| EaeI | YGGCCR | 4 cut(s) | 32, 992, 1033, 1851 |
| EagI | CGGCCG | 1 cut(s) | 1033 |
| Eam1104I | CTCTTC | 5 cut(s) | 450, 566, 753, 788, 832 |
| EarI | CTCTTC | 5 cut(s) | 450, 566, 753, 788, 832 |
| Ecl136II | GAGCTC | 2 cut(s) | 443, 676 |
| EclXI | CGGCCG | 1 cut(s) | 1033 |
| Eco130I | CCWWGG | 6 cut(s) | 35, 77, 115, 322, 816, 945 |
| Eco24I | GRGCYC | 3 cut(s) | 349, 445, 678 |
| Eco31I | GGTCTC | 1 cut(s) | 1214 |
| Eco32I | GATATC | 1 cut(s) | 209 |
| Eco47I | GGWCC | 3 cut(s) | 119, 407, 768 |
| Eco47III | AGCGCT | 1 cut(s) | 1061 |
| Eco52I | CGGCCG | 1 cut(s) | 1033 |
| Eco53kI | GAGCTC | 2 cut(s) | 443, 676 |
| Eco88I | CYCGRG | 1 cut(s) | 1670 |
| EcoICRI | GAGCTC | 2 cut(s) | 443, 676 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| EcoO109I | RGGNCCY | 1 cut(s) | 119 |
| EcoRI | GAATTC | 1 cut(s) | 254 |
| EcoRII | CCWGG | 3 cut(s) | 13, 340, 1419 |
| EcoRV | GATATC | 1 cut(s) | 209 |
| EcoT14I | CCWWGG | 6 cut(s) | 35, 77, 115, 322, 816, 945 |
| EcoT38I | GRGCYC | 3 cut(s) | 349, 445, 678 |
| ErhI | CCWWGG | 6 cut(s) | 35, 77, 115, 322, 816, 945 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1152, 1184, 1341, 1373 |
| FaqI | GGGAC | 3 cut(s) | 261, 610, 1354 |
| FblI | GTMKAC | 2 cut(s) | 240, 267 |
| Fnu4HI | GCNGC | 5 cut(s) | 55, 328, 1016, 1058, 1512 |
| FokI | GGATG | 5 cut(s) | 184, 299, 542, 1304, 1304 |
| FriOI | GRGCYC | 3 cut(s) | 349, 445, 678 |
| Fsp4HI | GCNGC | 5 cut(s) | 55, 328, 1016, 1058, 1512 |
| FspBI | CTAG | 1 cut(s) | 323 |
| FspI | TGCGCA | 1 cut(s) | 1619 |
| GlaI | GCGC | 3 cut(s) | 1061, 1619, 1727 |
| GluI | GCNGC | 5 cut(s) | 55, 328, 1016, 1058, 1512 |
| GsuI | CTGGAG | 1 cut(s) | 1629 |
| HaeII | RGCGCY | 1 cut(s) | 1063 |
| HaeIII | GGCC | 7 cut(s) | 34, 231, 950, 994, 1035, 1248, 1853 |
| HapII | CCGG | 5 cut(s) | 232, 405, 410, 766, 1734 |
| HgaI | GACGC | 1 cut(s) | 49 |
| HhaI | GCGC | 3 cut(s) | 1062, 1620, 1728 |
| Hin1I | GRCGYC | 1 cut(s) | 41 |
| Hin6I | GCGC | 3 cut(s) | 1060, 1618, 1726 |
| HinP1I | GCGC | 3 cut(s) | 1060, 1618, 1726 |
| HincII | GTYRAC | 4 cut(s) | 112, 241, 268, 969 |
| HindII | GTYRAC | 4 cut(s) | 112, 241, 268, 969 |
| HindIII | AAGCTT | 1 cut(s) | 1454 |
| HpaI | GTTAAC | 1 cut(s) | 112 |
| HpaII | CCGG | 5 cut(s) | 232, 405, 410, 766, 1734 |
| HphI | GGTGA | 6 cut(s) | 440, 493, 518, 589, 743, 1723 |
| Hpy166II | GTNNAC | 8 cut(s) | 88, 112, 241, 268, 591, 729, 969, 1258 |
| Hpy188III | TCNNGA | 4 cut(s) | 204, 766, 1213, 1672 |
| Hpy8I | GTNNAC | 8 cut(s) | 88, 112, 241, 268, 591, 729, 969, 1258 |
| Hpy99I | CGWCG | 1 cut(s) | 242 |
| HpyAV | CCTTC | 3 cut(s) | 1600, 1701, 1748 |
| HpyCH4III | ACNGT | 3 cut(s) | 1005, 1106, 1244 |
| HpyCH4IV | ACGT | 5 cut(s) | 63, 389, 965, 971, 1664 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 40, 947, 1626 |
| HpyF3I | CTNAG | 8 cut(s) | 954, 1176, 1303, 1358, 1374, 1479, 1750, 1860 |
| HpySE526I | ACGT | 5 cut(s) | 63, 389, 965, 971, 1664 |
| Hsp92I | GRCGYC | 1 cut(s) | 41 |
| HspAI | GCGC | 3 cut(s) | 1060, 1618, 1726 |
| Kpn2I | TCCGGA | 1 cut(s) | 765 |
| KspAI | GTTAAC | 1 cut(s) | 112 |
| Kzo9I | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| LguI | GCTCTTC | 1 cut(s) | 450 |
| LmnI | GCTCC | 3 cut(s) | 79, 180, 1228 |
| Lsp1109I | GCAGC | 5 cut(s) | 41, 339, 1002, 1069, 1498 |
| LweI | GCATC | 2 cut(s) | 162, 895 |
| MaeI | CTAG | 1 cut(s) | 323 |
| MaeII | ACGT | 5 cut(s) | 63, 389, 965, 971, 1664 |
| MaeIII | GTNAC | 6 cut(s) | 499, 577, 628, 997, 1665, 1778 |
| MalI | GATC | 5 cut(s) | 132, 194, 236, 613, 1520 |
| MboI | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| MboII | GAAGA | 9 cut(s) | 437, 553, 740, 775, 819, 840, 1346, 1562, 1666 |
| MfeI | CAATTG | 1 cut(s) | 1556 |
| MflI | RGATCY | 3 cut(s) | 130, 192, 1518 |
| MhlI | GDGCHC | 5 cut(s) | 349, 445, 663, 678, 1770 |
| MlsI | TGGCCA | 2 cut(s) | 34, 994 |
| MluCI | AATT | 9 cut(s) | 254, 667, 761, 772, 1076, 1556, 1599, 1613, 1868 |
| MluNI | TGGCCA | 2 cut(s) | 34, 994 |
| MlyI | GAGTC | 2 cut(s) | 243, 1651 |
| MmeI | TCCRAC | 4 cut(s) | 589, 1739, 1815, 1858 |
| Mox20I | TGGCCA | 2 cut(s) | 34, 994 |
| MroI | TCCGGA | 1 cut(s) | 765 |
| MscI | TGGCCA | 2 cut(s) | 34, 994 |
| MseI | TTAA | 4 cut(s) | 111, 714, 1074, 1871 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1773 |
| Msp20I | TGGCCA | 2 cut(s) | 34, 994 |
| MspA1I | CMGCKG | 1 cut(s) | 1273 |
| MspCI | CTTAAG | 1 cut(s) | 713 |
| MspI | CCGG | 5 cut(s) | 232, 405, 410, 766, 1734 |
| MspR9I | CCNGG | 3 cut(s) | 15, 342, 1421 |
| MunI | CAATTG | 1 cut(s) | 1556 |
| Mva1269I | GAATGC | 3 cut(s) | 18, 1329, 1567 |
| MvaI | CCWGG | 3 cut(s) | 15, 342, 1421 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 40, 947, 1626 |
| NcoI | CCATGG | 2 cut(s) | 35, 816 |
| NdeII | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| NlaIV | GGNNCC | 3 cut(s) | 75, 335, 814 |
| NmuCI | GTSAC | 5 cut(s) | 499, 577, 997, 1665, 1778 |
| NsbI | TGCGCA | 1 cut(s) | 1619 |
| NspI | RCATGY | 1 cut(s) | 1748 |
| NspV | TTCGAA | 1 cut(s) | 258 |
| OliI | CACNNNNGTG | 1 cut(s) | 1773 |
| PaeI | GCATGC | 1 cut(s) | 1748 |
| PaeR7I | CTCGAG | 1 cut(s) | 1670 |
| PaqCI | CACCTGC | 1 cut(s) | 345 |
| PciSI | GCTCTTC | 1 cut(s) | 450 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 264 |
| PctI | GAATGC | 3 cut(s) | 18, 1329, 1567 |
| PkrI | GCNGC | 5 cut(s) | 56, 329, 1017, 1059, 1513 |
| PleI | GAGTC | 2 cut(s) | 243, 1651 |
| PpsI | GAGTC | 2 cut(s) | 243, 1651 |
| Ppu21I | YACGTR | 1 cut(s) | 64 |
| PpuMI | RGGWCCY | 1 cut(s) | 119 |
| Psp124BI | GAGCTC | 2 cut(s) | 445, 678 |
| Psp5II | RGGWCCY | 1 cut(s) | 119 |
| Psp6I | CCWGG | 3 cut(s) | 13, 340, 1419 |
| PspGI | CCWGG | 3 cut(s) | 13, 340, 1419 |
| PspN4I | GGNNCC | 3 cut(s) | 75, 335, 814 |
| PspPI | GGNCC | 3 cut(s) | 119, 407, 768 |
| PspPPI | RGGWCCY | 1 cut(s) | 119 |
| PsuI | RGATCY | 3 cut(s) | 130, 192, 1518 |
| PvuII | CAGCTG | 1 cut(s) | 1273 |
| RsaI | GTAC | 4 cut(s) | 62, 719, 728, 1115 |
| RsaNI | GTAC | 4 cut(s) | 61, 718, 727, 1114 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1773 |
| Rsr2I | CGGWCCG | 1 cut(s) | 407 |
| RsrII | CGGWCCG | 1 cut(s) | 407 |
| SacI | GAGCTC | 2 cut(s) | 445, 678 |
| SalI | GTCGAC | 2 cut(s) | 239, 266 |
| SapI | GCTCTTC | 1 cut(s) | 450 |
| SaqAI | TTAA | 4 cut(s) | 111, 714, 1074, 1871 |
| SatI | GCNGC | 5 cut(s) | 55, 328, 1016, 1058, 1512 |
| Sau3AI | GATC | 5 cut(s) | 130, 192, 234, 611, 1518 |
| Sau96I | GGNCC | 3 cut(s) | 119, 407, 768 |
| ScaI | AGTACT | 1 cut(s) | 1115 |
| SchI | GAGTC | 2 cut(s) | 243, 1651 |
| ScrFI | CCNGG | 3 cut(s) | 15, 342, 1421 |
| SduI | GDGCHC | 5 cut(s) | 349, 445, 663, 678, 1770 |
| SfaNI | GCATC | 2 cut(s) | 162, 895 |
| SfcI | CTRYAG | 1 cut(s) | 930 |
| Sfr274I | CTCGAG | 1 cut(s) | 1670 |
| SfuI | TTCGAA | 1 cut(s) | 258 |
| SinI | GGWCC | 3 cut(s) | 119, 407, 768 |
| SlaI | CTCGAG | 1 cut(s) | 1670 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1773 |
| SmlI | CTYRAG | 2 cut(s) | 713, 1670 |
| SmoI | CTYRAG | 2 cut(s) | 713, 1670 |
| SphI | GCATGC | 1 cut(s) | 1748 |
| Sse9I | AATT | 9 cut(s) | 254, 667, 761, 772, 1076, 1556, 1599, 1613, 1868 |
| SsiI | CCGC | 2 cut(s) | 216, 536 |
| SspI | AATATT | 2 cut(s) | 300, 312 |
| SspMI | CTAG | 1 cut(s) | 323 |
| SstI | GAGCTC | 2 cut(s) | 445, 678 |
| StyD4I | CCNGG | 3 cut(s) | 13, 340, 1419 |
| StyI | CCWWGG | 6 cut(s) | 35, 77, 115, 322, 816, 945 |
| TaaI | ACNGT | 3 cut(s) | 1005, 1106, 1244 |
| TaiI | ACGT | 5 cut(s) | 66, 392, 968, 974, 1667 |
| TaqI | TCGA | 8 cut(s) | 211, 240, 252, 258, 267, 672, 1671, 1822 |
| TasI | AATT | 9 cut(s) | 254, 667, 761, 772, 1076, 1556, 1599, 1613, 1868 |
| TatI | WGTACW | 2 cut(s) | 726, 1113 |
| Tru1I | TTAA | 4 cut(s) | 111, 714, 1074, 1871 |
| Tru9I | TTAA | 4 cut(s) | 111, 714, 1074, 1871 |
| TscAI | CASTG | 4 cut(s) | 668, 1008, 1483, 1643 |
| TseFI | GTSAC | 5 cut(s) | 499, 577, 997, 1665, 1778 |
| TseI | GCWGC | 5 cut(s) | 54, 327, 1015, 1057, 1511 |
| Tsp45I | GTSAC | 5 cut(s) | 499, 577, 997, 1665, 1778 |
| TspDTI | ATGAA | 8 cut(s) | 94, 148, 389, 544, 662, 775, 858, 1667 |
| TspGWI | ACGGA | 1 cut(s) | 321 |
| TspRI | CASTG | 4 cut(s) | 668, 1008, 1483, 1643 |
| Vha464I | CTTAAG | 1 cut(s) | 713 |
| VpaK11BI | GGWCC | 3 cut(s) | 119, 407, 768 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| XapI | RAATTY | 2 cut(s) | 254, 1599 |
| XceI | RCATGY | 1 cut(s) | 1748 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 162, 1002 |
| XhoI | CTCGAG | 1 cut(s) | 1670 |
| XmaJI | CCTAGG | 1 cut(s) | 322 |
| XmiI | GTMKAC | 2 cut(s) | 240, 267 |
| XspI | CTAG | 1 cut(s) | 323 |
| ZrmI | AGTACT | 1 cut(s) | 1115 |