Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000300

Physical Location & Seq

Genomic Coordinates Reverse (-)

32786750 .. 32792650

Gene Structure 5901 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00310710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGAGTCATGTACACCTATAAAAGATCAGACGAGGAAGTTAATCAAGGCACAGTTGGTAAAAAAAATGGCTTCCAATCCTGGCATTCTGACTGAATGGCCATGGACACCTCTTGGAAACTTTAAGTACGTGGTGGTGGCTCCCTGGGCGATACACAGCATATACTCGTTCATTGTTAATGATGGAGAAGACAGAGATCTATCTTACTTCCTCATATTTCCGTTCATGTTGTTTAGGATGCTGCACAACCAGTTATGGATCTCTGTTTCTCGATATAAAACCTCCAAAGGCAGCGGCCGGATCATCGACAAGGGCCTGGAGTTCGAACAAGTTGACAGAGAAAGAAACTGGGATGACCAAATTATATTCAATGGACTATTGTTCTTCCTAGGCAGCAGGCACTTGACCGGAGGTCGAAACCTACCGATGTGGAGAGCAGATGGATTCGTTATAACATTTCTGCTTCATGCTGGTCCGGTGGAGTTTCTCTACTACTGGTTGCATAGAGCTCTGCACCACCATTACCTTTACTCTCGCTACCATTCTCACCACCATTCCTCCATTGTCACTGAGCCAATTACTTCTGTAATTCACCCATTTGGGGAGCACATAGCATATTTCCTGCTTTTCGCAATACCCTTGTACACAATGGCGTTGAAGGGAACAGGTTCAATCATATCCTATGCAGTTTATGTTACTTATATTGACTTAATGAACAACATGGGACACTGCAACTTCGAGCTCATTCCAAATTGGATCTTCTCTCTTTTTCCTCCACTCAAGTATTTCATATATACCCCATCGTACCACTCTTTGCATCACACCCTATTTCGAACCAATTATTGCCTCTTCATGCCACTTTATGATTACATTTATGGCACCATGGAAAAGACTAGTGATGAACTGTATGAATCTTCACTAAAAAGAAAAGATGAAGCTCCAGATGTGTTGCATCTAACCCATCTTACAACACCCGACTCCATCTATCAACTCCCTTTGGGATTTGCTTCCTTGGCCTCTGAGCCGCACACTTCAACATGGTACATGTGGTTGATGTGGCCTGTTACATTTTGGTCGATGATGCTCACATGGATTTATGGTCGCACATTTGTGGTTGAGAGGCAGCGGTTTGATAAACTCAAATTACAAACTTGGGCGATCCCAAAATACAATTTGCAATACTTCATGCCATGGCAAAATGAAGTTATCAACAGTTTGATTGAGGAAGCTATACTTAAAGCAGAGGAAAAGGGTGTCAAAGTTGTAAGTTTGGGGCTCTTAAACCAGGGTGAGGAGCTGAATAGATATGGTGGTCTATATATTCGGAGATATCCTCAGCTGAAAGTAAAGGTAGTGGATGGAAGTAGCTTAGCTGTGGCTGTAATCCTCAACAGCATACCAAAAGAGACAACCCAAGTTGTTCTTACAGGCAACCTTACAAAGGTCGCTTATGCAATTGCATTTGCTTTGAGCCAGAGGGGCATTCAGGTAGCTACATTATACAAGGATGAGTATTTGAAGCTCACCAAATCATTAAGCGCTACTAAGAGTGAGGTGGTTCTTGCAAAACGCTCTGCTTCAAAGATTTGGTTAGTGGGAGATGGATTGAGTAAAGAAGACCAACTAAATGCACCAAAAGGAACAAAGTTTGTTCCCTTCTCCCAATTCCCCCTAAAAAAGTTGCGCAGGGACTGCTTCTACCACTACACTCCAGCAATGAAGATCCCCAAGTCTCTTGAGAATGTTTACTCGTGTGAGAACTGGTTGCCGAGAAGGGTAATGAGCGCATGGCGCATAGCTGGAATAGTGCATGCCTTGGAAGGTTGGAATGAACATGAGTGTGGTTACGCCATGTCCGACATTGACAAGGTTTGGGAAGCTACGCTTCGACATGGCTTTCAGCCTCTGATCCTCAATGCTTCTCCGAACAAATATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016491 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko00073 ko01110 map00073 map01110

Pfam Domains

Protein Families

FA_hydroxylase Wax2_C

Protein Analysis

645

Amino Acids

74.93

Weight (kDa)

9.0

Isoelectric Point (pI)

33.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FA_hydroxylase	PF04116	155 - 294	3.6e-20	Fatty acid hydroxylase
CER1-like_C	PF12076	473 - 635	2.6e-73	CER1-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca	FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica	MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica	Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis	pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis	RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata	RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora	Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii	Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa	Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha	Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana	Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	452
Acc16I	TGCGCA	1 cut(s)	1685
AccB1I	GGYRCC	1 cut(s)	878
AciI	CCGC	3 cut(s)	294, 1025, 1126
AclWI	GGATC	6 cut(s)	266, 308, 764, 1153, 1717, 1903
AcoI	YGGCCR	2 cut(s)	98, 295
AfaI	GTAC	5 cut(s)	13, 128, 644, 806, 1043
AfeI	AGCGCT	1 cut(s)	1540
AfiI	CCNNNNNNNGG	3 cut(s)	601, 1291, 1441
AflIII	ACRYGT	1 cut(s)	1044
AgsI	TTSAA	6 cut(s)	370, 658, 672, 1035, 1519, 1581
AhdI	GACNNNNNGTC	1 cut(s)	411
AhlI	ACTAGT	1 cut(s)	893
AjnI	CCWGG	4 cut(s)	79, 143, 315, 1284
AleI	CACNNNNGTG	1 cut(s)	1109
Alw21I	GWGCWC	3 cut(s)	511, 609, 744
Alw26I	GTCTC	2 cut(s)	1400, 1737
AlwI	GGATC	6 cut(s)	266, 308, 764, 1153, 1717, 1903
AlwNI	CAGNNNCTG	2 cut(s)	1692, 1906
Aor51HI	AGCGCT	1 cut(s)	1540
AoxI	GGCC	5 cut(s)	98, 295, 313, 1014, 1058
ApeKI	GCWGC	4 cut(s)	241, 291, 393, 1123
Asp700I	GAANNNNTTC	1 cut(s)	667
AspA2I	CCTAGG	1 cut(s)	388
AspLEI	GCGC	4 cut(s)	1541, 1686, 1787, 1794
AspS9I	GGNCC	2 cut(s)	313, 473
AsuHPI	GGTGA	4 cut(s)	539, 584, 1301, 1516
AsuII	TTCGAA	2 cut(s)	324, 832
AvaII	GGWCC	1 cut(s)	473
AvrII	CCTAGG	1 cut(s)	388
BalI	TGGCCA	1 cut(s)	100
BanI	GGYRCC	1 cut(s)	878
BanII	GRGCYC	3 cut(s)	511, 744, 1278
BauI	CACGAG	1 cut(s)	1750
BbsI	GAAGAC	2 cut(s)	195, 1623
Bbv12I	GWGCWC	3 cut(s)	511, 609, 744
BbvCI	CCTCAGC	1 cut(s)	1335
BbvI	GCAGC	4 cut(s)	228, 303, 405, 1135
BccI	CCATC	7 cut(s)	176, 434, 808, 969, 989, 1352, 1595
BciT130I	CCWGG	4 cut(s)	81, 145, 317, 1286
BcoDI	GTCTC	2 cut(s)	1400, 1737
BcuI	ACTAGT	1 cut(s)	893
BfaI	CTAG	2 cut(s)	389, 894
BfoI	RGCGCY	1 cut(s)	1542
BglI	GCCNNNNNGGC	1 cut(s)	1479
BglII	AGATCT	1 cut(s)	196
BisI	GCNGC	6 cut(s)	242, 292, 295, 394, 1025, 1124
BlnI	CCTAGG	1 cut(s)	388
BlpI	GCTNAGC	1 cut(s)	1369
BlsI	GCNGC	6 cut(s)	243, 293, 296, 395, 1026, 1125
Bme1390I	CCNGG	4 cut(s)	81, 145, 317, 1286
Bme18I	GGWCC	1 cut(s)	473
BmeRI	GACNNNNNGTC	1 cut(s)	411
BmgT120I	GGNCC	2 cut(s)	313, 473
BmiI	GGNNCC	2 cut(s)	141, 880
BmrFI	CCNGG	4 cut(s)	81, 145, 317, 1286
BmrI	ACTGGG	1 cut(s)	358
BmsI	GCATC	4 cut(s)	228, 826, 961, 1071
BmuI	ACTGGG	1 cut(s)	358
BpiI	GAAGAC	2 cut(s)	195, 1623
BplI	GAGNNNNNCTC	2 cut(s)	1318, 1350
BpmI	CTGGAG	3 cut(s)	338, 924, 1695
Bpu10I	CCTNAGC	1 cut(s)	1335
Bpu1102I	GCTNAGC	1 cut(s)	1369
Bpu14I	TTCGAA	2 cut(s)	324, 832
BpuEI	CTTGAG	2 cut(s)	764, 1757
BsaAI	YACGTR	1 cut(s)	130
BsaJI	CCNNGG	9 cut(s)	101, 143, 144, 388, 882, 1011, 1190, 1285, 1815
BsaWI	WCCGGW	2 cut(s)	407, 475
BsaXI	ACNNNNNCTCC	2 cut(s)	542, 572
Bsc4I	CCNNNNNNNGG	3 cut(s)	601, 1291, 1441
Bse1I	ACTGG	4 cut(s)	250, 353, 500, 1766
Bse3DI	GCAATG	1 cut(s)	1722
BseBI	CCWGG	4 cut(s)	81, 145, 317, 1286
BseDI	CCNNGG	9 cut(s)	101, 143, 144, 388, 882, 1011, 1190, 1285, 1815
BseGI	GGATG	4 cut(s)	243, 358, 1363, 1513
BseLI	CCNNNNNNNGG	3 cut(s)	601, 1291, 1441
BseMI	GCAATG	1 cut(s)	1722
BseMII	CTCAG	3 cut(s)	561, 1011, 1349
BseNI	ACTGG	4 cut(s)	250, 353, 500, 1766
BseRI	GAGGAG	1 cut(s)	1307
BseX3I	CGGCCG	1 cut(s)	295
BseXI	GCAGC	4 cut(s)	228, 303, 405, 1135
BsgI	GTGCAG	2 cut(s)	227, 497
Bsh1285I	CGRYCG	1 cut(s)	298
BshFI	GGCC	5 cut(s)	100, 297, 315, 1016, 1060
BshNI	GGYRCC	1 cut(s)	878
BsiEI	CGRYCG	1 cut(s)	298
BsiHKAI	GWGCWC	3 cut(s)	511, 609, 744
BsiSI	CCGG	3 cut(s)	298, 408, 476
BslFI	GGGAC	2 cut(s)	738, 1703
BslI	CCNNNNNNNGG	3 cut(s)	601, 1291, 1441
BsmAI	GTCTC	2 cut(s)	1400, 1737
BsmFI	GGGAC	2 cut(s)	738, 1703
BsmI	GAATGC	2 cut(s)	84, 1482
BsnI	GGCC	5 cut(s)	100, 297, 315, 1016, 1060
Bsp119I	TTCGAA	2 cut(s)	324, 832
Bsp1286I	GDGCHC	4 cut(s)	511, 609, 744, 1278
Bsp1407I	TGTACA	2 cut(s)	11, 642
Bsp143I	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
Bsp1720I	GCTNAGC	1 cut(s)	1369
Bsp19I	CCATGG	3 cut(s)	101, 882, 1190
BspACI	CCGC	3 cut(s)	294, 1025, 1126
BspANI	GGCC	5 cut(s)	100, 297, 315, 1016, 1060
BspCNI	CTCAG	3 cut(s)	562, 1012, 1348
BspLI	GGNNCC	2 cut(s)	141, 880
BspPI	GGATC	6 cut(s)	266, 308, 764, 1153, 1717, 1903
BspT104I	TTCGAA	2 cut(s)	324, 832
BspT107I	GGYRCC	1 cut(s)	878
BsrDI	GCAATG	1 cut(s)	1722
BsrGI	TGTACA	2 cut(s)	11, 642
BsrI	ACTGG	4 cut(s)	250, 353, 500, 1766
BssECI	CCNNGG	9 cut(s)	101, 143, 144, 388, 882, 1011, 1190, 1285, 1815
BssMI	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
BssSI	CACGAG	1 cut(s)	1750
BssT1I	CCWWGG	6 cut(s)	101, 388, 882, 1011, 1190, 1815
Bst2BI	CACGAG	1 cut(s)	1750
Bst2UI	CCWGG	4 cut(s)	81, 145, 317, 1286
Bst4CI	ACNGT	3 cut(s)	54, 906, 1214
Bst6I	CTCTTC	1 cut(s)	854
BstAPI	GCANNNNNTGC	1 cut(s)	1692
BstAUI	TGTACA	2 cut(s)	11, 642
BstBAI	YACGTR	1 cut(s)	130
BstBI	TTCGAA	2 cut(s)	324, 832
BstC8I	GCNNGC	2 cut(s)	398, 1812
BstDEI	CTNAG	5 cut(s)	570, 1020, 1335, 1369, 1545
BstDSI	CCRYGG	3 cut(s)	101, 882, 1190
BstENI	CCTNNNNNAGG	1 cut(s)	1439
BstF5I	GGATG	4 cut(s)	243, 358, 1363, 1513
BstH2I	RGCGCY	1 cut(s)	1542
BstHHI	GCGC	4 cut(s)	1541, 1686, 1787, 1794
BstKTI	GATC	8 cut(s)	28, 199, 261, 303, 759, 1161, 1725, 1911
BstMAI	GTCTC	2 cut(s)	1400, 1737
BstMBI	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
BstMCI	CGRYCG	1 cut(s)	298
BstMWI	GCNNNNNNNGC	5 cut(s)	146, 1013, 1479, 1692, 1791
BstNI	CCWGG	4 cut(s)	81, 145, 317, 1286
BstNSI	RCATGY	2 cut(s)	1048, 1814
BstSCI	CCNGG	4 cut(s)	79, 143, 315, 1284
BstV1I	GCAGC	4 cut(s)	228, 303, 405, 1135
BstV2I	GAAGAC	2 cut(s)	195, 1623
BstX2I	RGATCY	4 cut(s)	196, 258, 756, 1722
BstYI	RGATCY	4 cut(s)	196, 258, 756, 1722
BstZI	CGGCCG	1 cut(s)	295
BsuRI	GGCC	5 cut(s)	100, 297, 315, 1016, 1060
BtgI	CCRYGG	3 cut(s)	101, 882, 1190
BtsCI	GGATG	4 cut(s)	243, 358, 1363, 1513
BtsI	GCAGTG	1 cut(s)	727
BtsIMutI	CAGTG	2 cut(s)	567, 727
Cac8I	GCNNGC	2 cut(s)	398, 1812
CaiI	CAGNNNCTG	2 cut(s)	1692, 1906
CfoI	GCGC	4 cut(s)	1541, 1686, 1787, 1794
Cfr13I	GGNCC	2 cut(s)	313, 473
Csp6I	GTAC	5 cut(s)	12, 127, 643, 805, 1042
CviQI	GTAC	5 cut(s)	12, 127, 643, 805, 1042
DdeI	CTNAG	5 cut(s)	570, 1020, 1335, 1369, 1545
DpnI	GATC	8 cut(s)	27, 198, 260, 302, 758, 1160, 1724, 1910
DpnII	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
DriI	GACNNNNNGTC	1 cut(s)	411
EaeI	YGGCCR	2 cut(s)	98, 295
EagI	CGGCCG	1 cut(s)	295
Eam1104I	CTCTTC	1 cut(s)	854
Eam1105I	GACNNNNNGTC	1 cut(s)	411
EarI	CTCTTC	1 cut(s)	854
Ecl136II	GAGCTC	2 cut(s)	509, 742
EclXI	CGGCCG	1 cut(s)	295
Eco130I	CCWWGG	6 cut(s)	101, 388, 882, 1011, 1190, 1815
Eco24I	GRGCYC	3 cut(s)	511, 744, 1278
Eco32I	GATATC	1 cut(s)	1331
Eco47I	GGWCC	1 cut(s)	473
Eco47III	AGCGCT	1 cut(s)	1540
Eco52I	CGGCCG	1 cut(s)	295
Eco53kI	GAGCTC	2 cut(s)	509, 742
EcoICRI	GAGCTC	2 cut(s)	509, 742
EcoNI	CCTNNNNNAGG	1 cut(s)	1439
EcoO109I	RGGNCCY	1 cut(s)	313
EcoRII	CCWGG	4 cut(s)	79, 143, 315, 1284
EcoRV	GATATC	1 cut(s)	1331
EcoT14I	CCWWGG	6 cut(s)	101, 388, 882, 1011, 1190, 1815
EcoT38I	GRGCYC	3 cut(s)	511, 744, 1278
ErhI	CCWWGG	6 cut(s)	101, 388, 882, 1011, 1190, 1815
FalI	AAGNNNNNCTT	6 cut(s)	1218, 1250, 1407, 1439, 1869, 1901
FaqI	GGGAC	2 cut(s)	738, 1703
Fnu4HI	GCNGC	6 cut(s)	242, 292, 295, 394, 1025, 1124
FokI	GGATG	4 cut(s)	250, 365, 1370, 1520
FriOI	GRGCYC	3 cut(s)	511, 744, 1278
Fsp4HI	GCNGC	6 cut(s)	242, 292, 295, 394, 1025, 1124
FspBI	CTAG	2 cut(s)	389, 894
FspI	TGCGCA	1 cut(s)	1685
GlaI	GCGC	4 cut(s)	1540, 1685, 1786, 1793
GluI	GCNGC	6 cut(s)	242, 292, 295, 394, 1025, 1124
GsuI	CTGGAG	3 cut(s)	338, 924, 1695
HaeII	RGCGCY	1 cut(s)	1542
HaeIII	GGCC	5 cut(s)	100, 297, 315, 1016, 1060
HapII	CCGG	3 cut(s)	298, 408, 476
HhaI	GCGC	4 cut(s)	1541, 1686, 1787, 1794
Hin6I	GCGC	4 cut(s)	1539, 1684, 1785, 1792
HinP1I	GCGC	4 cut(s)	1539, 1684, 1785, 1792
HincII	GTYRAC	1 cut(s)	334
HindII	GTYRAC	1 cut(s)	334
HinfI	GANTC	4 cut(s)	5, 444, 911, 977
HpaII	CCGG	3 cut(s)	298, 408, 476
HphI	GGTGA	4 cut(s)	539, 584, 1301, 1516
Hpy166II	GTNNAC	4 cut(s)	14, 334, 645, 1747
Hpy188I	TCNGA	7 cut(s)	30, 90, 1021, 1326, 1858, 1908, 1926
Hpy188III	TCNNGA	3 cut(s)	270, 941, 1736
Hpy8I	GTNNAC	4 cut(s)	14, 334, 645, 1747
HpyAV	CCTTC	4 cut(s)	652, 1666, 1767, 1814
HpyCH4III	ACNGT	3 cut(s)	54, 906, 1214
HpyCH4IV	ACGT	1 cut(s)	129
HpyF10VI	GCNNNNNNNGC	5 cut(s)	146, 1013, 1479, 1692, 1791
HpyF3I	CTNAG	5 cut(s)	570, 1020, 1335, 1369, 1545
HpySE526I	ACGT	1 cut(s)	129
HspAI	GCGC	4 cut(s)	1539, 1684, 1785, 1792
Kzo9I	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
LmnI	GCTCC	4 cut(s)	145, 604, 943, 1294
Lsp1109I	GCAGC	4 cut(s)	228, 303, 405, 1135
LweI	GCATC	4 cut(s)	228, 826, 961, 1071
MaeI	CTAG	2 cut(s)	389, 894
MaeII	ACGT	1 cut(s)	129
MaeIII	GTNAC	4 cut(s)	565, 694, 1063, 1844
MalI	GATC	8 cut(s)	27, 198, 260, 302, 758, 1160, 1724, 1910
MboI	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
MboII	GAAGA	7 cut(s)	200, 376, 751, 841, 906, 1628, 1732
MfeI	CAATTG	1 cut(s)	1455
MflI	RGATCY	4 cut(s)	196, 258, 756, 1722
MhlI	GDGCHC	4 cut(s)	511, 609, 744, 1278
MlsI	TGGCCA	1 cut(s)	100
MluCI	AATT	9 cut(s)	360, 576, 588, 751, 838, 1142, 1171, 1455, 1664
MluNI	TGGCCA	1 cut(s)	100
MlyI	GAGTC	2 cut(s)	14, 971
MmeI	TCCRAC	2 cut(s)	1805, 1881
Mox20I	TGGCCA	1 cut(s)	100
MroXI	GAANNNNTTC	1 cut(s)	667
MscI	TGGCCA	1 cut(s)	100
MseI	TTAA	8 cut(s)	41, 123, 177, 710, 1236, 1280, 1535, 1936
MslI	CAYNNNNRTG	2 cut(s)	1109, 1839
Msp20I	TGGCCA	1 cut(s)	100
MspA1I	CMGCKG	3 cut(s)	294, 1126, 1339
MspI	CCGG	3 cut(s)	298, 408, 476
MspR9I	CCNGG	4 cut(s)	81, 145, 317, 1286
MunI	CAATTG	1 cut(s)	1455
Mva1269I	GAATGC	2 cut(s)	84, 1482
MvaI	CCWGG	4 cut(s)	81, 145, 317, 1286
MwoI	GCNNNNNNNGC	5 cut(s)	146, 1013, 1479, 1692, 1791
NcoI	CCATGG	3 cut(s)	101, 882, 1190
NdeII	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
NlaIV	GGNNCC	2 cut(s)	141, 880
NmeAIII	GCCGAG	1 cut(s)	1794
NmuCI	GTSAC	1 cut(s)	565
NsbI	TGCGCA	1 cut(s)	1685
NspI	RCATGY	2 cut(s)	1048, 1814
NspV	TTCGAA	2 cut(s)	324, 832
OliI	CACNNNNGTG	1 cut(s)	1109
PaeI	GCATGC	1 cut(s)	1814
PasI	CCCWGGG	1 cut(s)	144
PciI	ACATGT	1 cut(s)	1044
PcsI	WCGNNNNNNNCGW	1 cut(s)	1854
PctI	GAATGC	2 cut(s)	84, 1482
PdmI	GAANNNNTTC	1 cut(s)	667
PfeI	GAWTC	2 cut(s)	444, 911
PkrI	GCNGC	6 cut(s)	243, 293, 296, 395, 1026, 1125
PleI	GAGTC	2 cut(s)	13, 971
PpsI	GAGTC	2 cut(s)	13, 971
Ppu21I	YACGTR	1 cut(s)	130
PscI	ACATGT	1 cut(s)	1044
PsiI	TTATAA	1 cut(s)	452
Psp124BI	GAGCTC	2 cut(s)	511, 744
Psp6I	CCWGG	4 cut(s)	79, 143, 315, 1284
PspGI	CCWGG	4 cut(s)	79, 143, 315, 1284
PspN4I	GGNNCC	2 cut(s)	141, 880
PspPI	GGNCC	2 cut(s)	313, 473
PstNI	CAGNNNCTG	2 cut(s)	1692, 1906
PsuI	RGATCY	4 cut(s)	196, 258, 756, 1722
PvuII	CAGCTG	1 cut(s)	1339
RsaI	GTAC	5 cut(s)	13, 128, 644, 806, 1043
RsaNI	GTAC	5 cut(s)	12, 127, 643, 805, 1042
RseI	CAYNNNNRTG	2 cut(s)	1109, 1839
SacI	GAGCTC	2 cut(s)	511, 744
SaqAI	TTAA	8 cut(s)	41, 123, 177, 710, 1236, 1280, 1535, 1936
SatI	GCNGC	6 cut(s)	242, 292, 295, 394, 1025, 1124
Sau3AI	GATC	8 cut(s)	25, 196, 258, 300, 756, 1158, 1722, 1908
Sau96I	GGNCC	2 cut(s)	313, 473
SchI	GAGTC	2 cut(s)	14, 971
ScrFI	CCNGG	4 cut(s)	81, 145, 317, 1286
SduI	GDGCHC	4 cut(s)	511, 609, 744, 1278
SfaNI	GCATC	4 cut(s)	228, 826, 961, 1071
SfuI	TTCGAA	2 cut(s)	324, 832
SinI	GGWCC	1 cut(s)	473
SmiMI	CAYNNNNRTG	2 cut(s)	1109, 1839
SmlI	CTYRAG	2 cut(s)	779, 1736
SmoI	CTYRAG	2 cut(s)	779, 1736
SpeI	ACTAGT	1 cut(s)	893
SphI	GCATGC	1 cut(s)	1814
Sse9I	AATT	9 cut(s)	360, 576, 588, 751, 838, 1142, 1171, 1455, 1664
SsiI	CCGC	3 cut(s)	294, 1025, 1126
SspI	AATATT	1 cut(s)	1934
SspMI	CTAG	2 cut(s)	389, 894
SstI	GAGCTC	2 cut(s)	511, 744
StyD4I	CCNGG	4 cut(s)	79, 143, 315, 1284
StyI	CCWWGG	6 cut(s)	101, 388, 882, 1011, 1190, 1815
TaaI	ACNGT	3 cut(s)	54, 906, 1214
TaiI	ACGT	1 cut(s)	132
TaqI	TCGA	8 cut(s)	271, 306, 324, 415, 738, 832, 1076, 1888
TasI	AATT	9 cut(s)	360, 576, 588, 751, 838, 1142, 1171, 1455, 1664
TatI	WGTACW	2 cut(s)	11, 642
TauI	GCSGC	2 cut(s)	297, 1027
TfiI	GAWTC	2 cut(s)	444, 911
Tru1I	TTAA	8 cut(s)	41, 123, 177, 710, 1236, 1280, 1535, 1936
Tru9I	TTAA	8 cut(s)	41, 123, 177, 710, 1236, 1280, 1535, 1936
TscAI	CASTG	2 cut(s)	574, 734
TseFI	GTSAC	1 cut(s)	565
TseI	GCWGC	4 cut(s)	241, 291, 393, 1123
Tsp45I	GTSAC	1 cut(s)	565
TspGWI	ACGGA	1 cut(s)	210
TspRI	CASTG	2 cut(s)	574, 734
VpaK11BI	GGWCC	1 cut(s)	473
XagI	CCTNNNNNAGG	1 cut(s)	1439
XceI	RCATGY	2 cut(s)	1048, 1814
XmaJI	CCTAGG	1 cut(s)	388
XmnI	GAANNNNTTC	1 cut(s)	667
XspI	CTAG	2 cut(s)	389, 894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.