Belongs to the sterol desaturase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1223 |
| Acc16I | TGCGCA | 1 cut(s) | 1643 |
| Acc65I | GGTACC | 2 cut(s) | 332, 999 |
| AccB1I | GGYRCC | 3 cut(s) | 332, 836, 999 |
| AccI | GTMKAC | 1 cut(s) | 752 |
| AciI | CCGC | 2 cut(s) | 560, 953 |
| AclWI | GGATC | 4 cut(s) | 193, 224, 1058, 1260 |
| AcoI | YGGCCR | 1 cut(s) | 32 |
| AcsI | RAATTY | 3 cut(s) | 18, 258, 278 |
| AfaI | GTAC | 8 cut(s) | 334, 344, 381, 443, 1001, 1034, 1063, 1430 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 94, 559, 1399 |
| AgsI | TTSAA | 6 cut(s) | 278, 328, 1196, 1240, 1283, 1477 |
| AjnI | CCWGG | 2 cut(s) | 13, 1443 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 344, 376 |
| Alw21I | GWGCWC | 3 cut(s) | 469, 567, 702 |
| Alw26I | GTCTC | 2 cut(s) | 1232, 1277 |
| AlwI | GGATC | 4 cut(s) | 193, 224, 1058, 1260 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 629, 1337 |
| AoxI | GGCC | 3 cut(s) | 32, 1016, 1231 |
| ApeKI | GCWGC | 1 cut(s) | 1081 |
| ApoI | RAATTY | 3 cut(s) | 18, 258, 278 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 625 |
| Asp718I | GGTACC | 2 cut(s) | 332, 999 |
| AspLEI | GCGC | 1 cut(s) | 1644 |
| AspS9I | GGNCC | 1 cut(s) | 431 |
| AsuHPI | GGTGA | 7 cut(s) | 464, 500, 542, 1259, 1474, 1747, 1861 |
| AsuNHI | GCTAGC | 1 cut(s) | 601 |
| AvaII | GGWCC | 1 cut(s) | 431 |
| BaeGI | GKGCMC | 1 cut(s) | 1591 |
| BalI | TGGCCA | 1 cut(s) | 34 |
| BanI | GGYRCC | 3 cut(s) | 332, 836, 999 |
| BanII | GRGCYC | 2 cut(s) | 373, 702 |
| BbsI | GAAGAC | 1 cut(s) | 1835 |
| Bbv12I | GWGCWC | 3 cut(s) | 469, 567, 702 |
| BbvI | GCAGC | 1 cut(s) | 1093 |
| BccI | CCATC | 6 cut(s) | 134, 483, 927, 947, 1310, 1553 |
| BciT130I | CCWGG | 2 cut(s) | 15, 1445 |
| BciVI | GTATCC | 1 cut(s) | 1451 |
| BcoDI | GTCTC | 2 cut(s) | 1232, 1277 |
| BfaI | CTAG | 2 cut(s) | 602, 956 |
| BfuI | GTATCC | 1 cut(s) | 1451 |
| BisI | GCNGC | 1 cut(s) | 1082 |
| BlpI | GCTNAGC | 1 cut(s) | 1327 |
| BlsI | GCNGC | 1 cut(s) | 1083 |
| Bme1390I | CCNGG | 2 cut(s) | 15, 1445 |
| Bme18I | GGWCC | 1 cut(s) | 431 |
| BmgT120I | GGNCC | 1 cut(s) | 431 |
| BmiI | GGNNCC | 4 cut(s) | 99, 334, 838, 1001 |
| BmrFI | CCNGG | 2 cut(s) | 15, 1445 |
| BmrI | ACTGGG | 1 cut(s) | 316 |
| BmsI | GCATC | 2 cut(s) | 784, 1297 |
| BmtI | GCTAGC | 1 cut(s) | 605 |
| BmuI | ACTGGG | 1 cut(s) | 316 |
| BoxI | GACNNNNGTC | 1 cut(s) | 270 |
| BpiI | GAAGAC | 1 cut(s) | 1835 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1377, 1409 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1327 |
| BpuEI | CTTGAG | 4 cut(s) | 722, 863, 1356, 1715 |
| BsaAI | YACGTR | 1 cut(s) | 829 |
| BsaBI | GATNNNNATC | 1 cut(s) | 215 |
| BsaI | GGTCTC | 1 cut(s) | 1277 |
| BsaJI | CCNNGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| BsaWI | WCCGGW | 1 cut(s) | 931 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1781, 1811 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 94, 559, 1399 |
| Bse1I | ACTGG | 1 cut(s) | 311 |
| Bse3DI | GCAATG | 1 cut(s) | 256 |
| Bse8I | GATNNNNATC | 1 cut(s) | 215 |
| BseBI | CCWGG | 2 cut(s) | 15, 1445 |
| BseDI | CCNNGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| BseGI | GGATG | 5 cut(s) | 165, 201, 316, 1288, 1321 |
| BseJI | GATNNNNATC | 1 cut(s) | 215 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 94, 559, 1399 |
| BseMI | GCAATG | 1 cut(s) | 256 |
| BseMII | CTCAG | 2 cut(s) | 1111, 1191 |
| BseNI | ACTGG | 1 cut(s) | 311 |
| BseRI | GAGGAG | 2 cut(s) | 723, 902 |
| BseSI | GKGCMC | 1 cut(s) | 1591 |
| BseXI | GCAGC | 1 cut(s) | 1093 |
| BseYI | CCCAGC | 1 cut(s) | 1669 |
| BshFI | GGCC | 3 cut(s) | 34, 1018, 1233 |
| BshNI | GGYRCC | 3 cut(s) | 332, 836, 999 |
| BsiHKAI | GWGCWC | 3 cut(s) | 469, 567, 702 |
| BsiSI | CCGG | 1 cut(s) | 932 |
| BslFI | GGGAC | 2 cut(s) | 696, 1378 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 94, 559, 1399 |
| BsmAI | GTCTC | 2 cut(s) | 1232, 1277 |
| BsmFI | GGGAC | 2 cut(s) | 696, 1378 |
| BsmI | GAATGC | 1 cut(s) | 1353 |
| BsnI | GGCC | 3 cut(s) | 34, 1018, 1233 |
| Bso31I | GGTCTC | 1 cut(s) | 1277 |
| Bsp1286I | GDGCHC | 5 cut(s) | 373, 469, 567, 702, 1591 |
| Bsp143I | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1327 |
| Bsp19I | CCATGG | 2 cut(s) | 35, 840 |
| BspACI | CCGC | 2 cut(s) | 560, 953 |
| BspANI | GGCC | 3 cut(s) | 34, 1018, 1233 |
| BspCNI | CTCAG | 2 cut(s) | 1110, 1192 |
| BspHI | TCATGA | 2 cut(s) | 129, 669 |
| BspLI | GGNNCC | 4 cut(s) | 99, 334, 838, 1001 |
| BspOI | GCTAGC | 1 cut(s) | 605 |
| BspPI | GGATC | 4 cut(s) | 193, 224, 1058, 1260 |
| BspQI | GCTCTTC | 1 cut(s) | 590 |
| BspT107I | GGYRCC | 3 cut(s) | 332, 836, 999 |
| BspTNI | GGTCTC | 1 cut(s) | 1277 |
| BsrDI | GCAATG | 1 cut(s) | 256 |
| BsrI | ACTGG | 1 cut(s) | 311 |
| BssECI | CCNNGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| BssMI | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| BssNAI | GTATAC | 1 cut(s) | 753 |
| BssT1I | CCWWGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| Bst1107I | GTATAC | 1 cut(s) | 753 |
| Bst2UI | CCWGG | 2 cut(s) | 15, 1445 |
| Bst4CI | ACNGT | 1 cut(s) | 610 |
| Bst6I | CTCTTC | 3 cut(s) | 590, 722, 812 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1650 |
| BstBAI | YACGTR | 1 cut(s) | 829 |
| BstC8I | GCNNGC | 4 cut(s) | 603, 1759, 1770, 1836 |
| BstDEI | CTNAG | 3 cut(s) | 1097, 1200, 1327 |
| BstDSI | CCRYGG | 2 cut(s) | 35, 840 |
| BstEII | GGTNACC | 1 cut(s) | 1388 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1397 |
| BstF5I | GGATG | 5 cut(s) | 165, 201, 316, 1288, 1321 |
| BstHHI | GCGC | 1 cut(s) | 1644 |
| BstKTI | GATC | 5 cut(s) | 201, 219, 286, 1053, 1255 |
| BstMAI | GTCTC | 2 cut(s) | 1232, 1277 |
| BstMBI | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 971, 1650 |
| BstNI | CCWGG | 2 cut(s) | 15, 1445 |
| BstNSI | RCATGY | 1 cut(s) | 1772 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 270 |
| BstPI | GGTNACC | 1 cut(s) | 1388 |
| BstSCI | CCNGG | 2 cut(s) | 13, 1443 |
| BstSLI | GKGCMC | 1 cut(s) | 1591 |
| BstSNI | TACGTA | 1 cut(s) | 829 |
| BstV1I | GCAGC | 1 cut(s) | 1093 |
| BstV2I | GAAGAC | 1 cut(s) | 1835 |
| BstX2I | RGATCY | 2 cut(s) | 1050, 1252 |
| BstYI | RGATCY | 2 cut(s) | 1050, 1252 |
| BstZ17I | GTATAC | 1 cut(s) | 753 |
| BsuI | GTATCC | 1 cut(s) | 1451 |
| BsuRI | GGCC | 3 cut(s) | 34, 1018, 1233 |
| BtgI | CCRYGG | 2 cut(s) | 35, 840 |
| BtgZI | GCGATG | 1 cut(s) | 1688 |
| BtsCI | GGATG | 5 cut(s) | 165, 201, 316, 1288, 1321 |
| BtsI | GCAGTG | 1 cut(s) | 685 |
| BtsIMutI | CAGTG | 3 cut(s) | 685, 1500, 1818 |
| Cac8I | GCNNGC | 4 cut(s) | 603, 1759, 1770, 1836 |
| CaiI | CAGNNNCTG | 2 cut(s) | 629, 1337 |
| CciI | TCATGA | 2 cut(s) | 129, 669 |
| CfoI | GCGC | 1 cut(s) | 1644 |
| Cfr13I | GGNCC | 1 cut(s) | 431 |
| Csp6I | GTAC | 8 cut(s) | 333, 343, 380, 442, 1000, 1033, 1062, 1429 |
| CviQI | GTAC | 8 cut(s) | 333, 343, 380, 442, 1000, 1033, 1062, 1429 |
| DdeI | CTNAG | 3 cut(s) | 1097, 1200, 1327 |
| DpnI | GATC | 5 cut(s) | 200, 218, 285, 1052, 1254 |
| DpnII | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| EaeI | YGGCCR | 1 cut(s) | 32 |
| Eam1104I | CTCTTC | 3 cut(s) | 590, 722, 812 |
| EarI | CTCTTC | 3 cut(s) | 590, 722, 812 |
| Ecl136II | GAGCTC | 1 cut(s) | 700 |
| Eco105I | TACGTA | 1 cut(s) | 829 |
| Eco130I | CCWWGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| Eco24I | GRGCYC | 2 cut(s) | 373, 702 |
| Eco31I | GGTCTC | 1 cut(s) | 1277 |
| Eco32I | GATATC | 2 cut(s) | 233, 904 |
| Eco47I | GGWCC | 1 cut(s) | 431 |
| Eco53kI | GAGCTC | 1 cut(s) | 700 |
| Eco91I | GGTNACC | 1 cut(s) | 1388 |
| EcoICRI | GAGCTC | 1 cut(s) | 700 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1397 |
| EcoO65I | GGTNACC | 1 cut(s) | 1388 |
| EcoRI | GAATTC | 3 cut(s) | 18, 258, 278 |
| EcoRII | CCWGG | 2 cut(s) | 13, 1443 |
| EcoRV | GATATC | 2 cut(s) | 233, 904 |
| EcoT14I | CCWWGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| EcoT38I | GRGCYC | 2 cut(s) | 373, 702 |
| ErhI | CCWWGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1827, 1859 |
| FaqI | GGGAC | 2 cut(s) | 696, 1378 |
| FblI | GTMKAC | 1 cut(s) | 752 |
| Fnu4HI | GCNGC | 1 cut(s) | 1082 |
| FokI | GGATG | 5 cut(s) | 152, 208, 323, 1275, 1328 |
| FriOI | GRGCYC | 2 cut(s) | 373, 702 |
| Fsp4HI | GCNGC | 1 cut(s) | 1082 |
| FspBI | CTAG | 2 cut(s) | 602, 956 |
| FspI | TGCGCA | 1 cut(s) | 1643 |
| GlaI | GCGC | 1 cut(s) | 1643 |
| GluI | GCNGC | 1 cut(s) | 1082 |
| GsaI | CCCAGC | 1 cut(s) | 1673 |
| HaeIII | GGCC | 3 cut(s) | 34, 1018, 1233 |
| HapII | CCGG | 1 cut(s) | 932 |
| HhaI | GCGC | 1 cut(s) | 1644 |
| Hin6I | GCGC | 1 cut(s) | 1642 |
| HinP1I | GCGC | 1 cut(s) | 1642 |
| HincII | GTYRAC | 2 cut(s) | 292, 1044 |
| HindII | GTYRAC | 2 cut(s) | 292, 1044 |
| HindIII | AAGCTT | 1 cut(s) | 52 |
| HinfI | GANTC | 7 cut(s) | 547, 857, 893, 935, 1149, 1240, 1681 |
| HpaI | GTTAAC | 1 cut(s) | 1044 |
| HpaII | CCGG | 1 cut(s) | 932 |
| HphI | GGTGA | 7 cut(s) | 464, 500, 542, 1259, 1474, 1747, 1861 |
| Hpy166II | GTNNAC | 3 cut(s) | 292, 753, 1044 |
| Hpy188I | TCNGA | 6 cut(s) | 791, 1050, 1100, 1257, 1385, 1816 |
| Hpy188III | TCNNGA | 9 cut(s) | 130, 228, 263, 275, 670, 880, 1153, 1237, 1694 |
| Hpy8I | GTNNAC | 3 cut(s) | 292, 753, 1044 |
| HpyAV | CCTTC | 7 cut(s) | 768, 917, 1429, 1624, 1725, 1772, 1780 |
| HpyCH4III | ACNGT | 1 cut(s) | 610 |
| HpyCH4IV | ACGT | 1 cut(s) | 828 |
| HpyCH4V | TGCA | 8 cut(s) | 410, 690, 775, 1135, 1523, 1535, 1745, 1768 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 971, 1650 |
| HpyF3I | CTNAG | 3 cut(s) | 1097, 1200, 1327 |
| HpySE526I | ACGT | 1 cut(s) | 828 |
| HspAI | GCGC | 1 cut(s) | 1642 |
| KpnI | GGTACC | 2 cut(s) | 336, 1003 |
| KspAI | GTTAAC | 1 cut(s) | 1044 |
| Kzo9I | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| LguI | GCTCTTC | 1 cut(s) | 590 |
| LmnI | GCTCC | 3 cut(s) | 103, 562, 1789 |
| Lsp1109I | GCAGC | 1 cut(s) | 1093 |
| LweI | GCATC | 2 cut(s) | 784, 1297 |
| MaeI | CTAG | 2 cut(s) | 602, 956 |
| MaeII | ACGT | 1 cut(s) | 828 |
| MaeIII | GTNAC | 5 cut(s) | 523, 1021, 1388, 1802, 1867 |
| MalI | GATC | 5 cut(s) | 200, 218, 285, 1052, 1254 |
| MboI | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| MboII | GAAGA | 6 cut(s) | 577, 709, 799, 1475, 1690, 1835 |
| MfeI | CAATTG | 1 cut(s) | 1168 |
| MflI | RGATCY | 2 cut(s) | 1050, 1252 |
| MhlI | GDGCHC | 5 cut(s) | 373, 469, 567, 702, 1591 |
| MlsI | TGGCCA | 1 cut(s) | 34 |
| MluNI | TGGCCA | 1 cut(s) | 34 |
| MmeI | TCCRAC | 4 cut(s) | 375, 1534, 1763, 1839 |
| Mox20I | TGGCCA | 1 cut(s) | 34 |
| MroXI | GAANNNNTTC | 1 cut(s) | 625 |
| MscI | TGGCCA | 1 cut(s) | 34 |
| MseI | TTAA | 4 cut(s) | 57, 1043, 1089, 1175 |
| MslI | CAYNNNNRTG | 3 cut(s) | 994, 1464, 1797 |
| Msp20I | TGGCCA | 1 cut(s) | 34 |
| MspI | CCGG | 1 cut(s) | 932 |
| MspR9I | CCNGG | 2 cut(s) | 15, 1445 |
| MunI | CAATTG | 1 cut(s) | 1168 |
| Mva1269I | GAATGC | 1 cut(s) | 1353 |
| MvaI | CCWGG | 2 cut(s) | 15, 1445 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 971, 1650 |
| NcoI | CCATGG | 2 cut(s) | 35, 840 |
| NdeII | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| NheI | GCTAGC | 1 cut(s) | 601 |
| NlaIV | GGNNCC | 4 cut(s) | 99, 334, 838, 1001 |
| NmeAIII | GCCGAG | 1 cut(s) | 1209 |
| NmuCI | GTSAC | 2 cut(s) | 1021, 1867 |
| NsbI | TGCGCA | 1 cut(s) | 1643 |
| NspI | RCATGY | 1 cut(s) | 1772 |
| PaeI | GCATGC | 1 cut(s) | 1772 |
| PagI | TCATGA | 2 cut(s) | 129, 669 |
| PciSI | GCTCTTC | 1 cut(s) | 590 |
| PctI | GAATGC | 1 cut(s) | 1353 |
| PdmI | GAANNNNTTC | 1 cut(s) | 625 |
| PfeI | GAWTC | 7 cut(s) | 547, 857, 893, 935, 1149, 1240, 1681 |
| PkrI | GCNGC | 1 cut(s) | 1083 |
| Ppu21I | YACGTR | 1 cut(s) | 829 |
| PshAI | GACNNNNGTC | 1 cut(s) | 270 |
| PsiI | TTATAA | 1 cut(s) | 1223 |
| Psp124BI | GAGCTC | 1 cut(s) | 702 |
| Psp6I | CCWGG | 2 cut(s) | 13, 1443 |
| PspEI | GGTNACC | 1 cut(s) | 1388 |
| PspFI | CCCAGC | 1 cut(s) | 1669 |
| PspGI | CCWGG | 2 cut(s) | 13, 1443 |
| PspN4I | GGNNCC | 4 cut(s) | 99, 334, 838, 1001 |
| PspPI | GGNCC | 1 cut(s) | 431 |
| PstNI | CAGNNNCTG | 2 cut(s) | 629, 1337 |
| PsuI | RGATCY | 2 cut(s) | 1050, 1252 |
| RsaI | GTAC | 8 cut(s) | 334, 344, 381, 443, 1001, 1034, 1063, 1430 |
| RsaNI | GTAC | 8 cut(s) | 333, 343, 380, 442, 1000, 1033, 1062, 1429 |
| RseI | CAYNNNNRTG | 3 cut(s) | 994, 1464, 1797 |
| SacI | GAGCTC | 1 cut(s) | 702 |
| SapI | GCTCTTC | 1 cut(s) | 590 |
| SaqAI | TTAA | 4 cut(s) | 57, 1043, 1089, 1175 |
| SatI | GCNGC | 1 cut(s) | 1082 |
| Sau3AI | GATC | 5 cut(s) | 198, 216, 283, 1050, 1252 |
| Sau96I | GGNCC | 1 cut(s) | 431 |
| ScrFI | CCNGG | 2 cut(s) | 15, 1445 |
| SduI | GDGCHC | 5 cut(s) | 373, 469, 567, 702, 1591 |
| SfaNI | GCATC | 2 cut(s) | 784, 1297 |
| SinI | GGWCC | 1 cut(s) | 431 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 994, 1464, 1797 |
| SmlI | CTYRAG | 4 cut(s) | 737, 878, 1371, 1694 |
| SmoI | CTYRAG | 4 cut(s) | 737, 878, 1371, 1694 |
| SnaBI | TACGTA | 1 cut(s) | 829 |
| SphI | GCATGC | 1 cut(s) | 1772 |
| SsiI | CCGC | 2 cut(s) | 560, 953 |
| SspI | AATATT | 3 cut(s) | 321, 1472, 1702 |
| SspMI | CTAG | 2 cut(s) | 602, 956 |
| SstI | GAGCTC | 1 cut(s) | 702 |
| StyD4I | CCNGG | 2 cut(s) | 13, 1443 |
| StyI | CCWWGG | 5 cut(s) | 35, 101, 840, 969, 1773 |
| TaaI | ACNGT | 1 cut(s) | 610 |
| TaiI | ACGT | 1 cut(s) | 831 |
| TaqI | TCGA | 6 cut(s) | 229, 235, 256, 282, 1147, 1846 |
| TfiI | GAWTC | 7 cut(s) | 547, 857, 893, 935, 1149, 1240, 1681 |
| Tru1I | TTAA | 4 cut(s) | 57, 1043, 1089, 1175 |
| Tru9I | TTAA | 4 cut(s) | 57, 1043, 1089, 1175 |
| TscAI | CASTG | 3 cut(s) | 692, 1507, 1825 |
| TseFI | GTSAC | 2 cut(s) | 1021, 1867 |
| TseI | GCWGC | 1 cut(s) | 1081 |
| Tsp45I | GTSAC | 2 cut(s) | 1021, 1867 |
| TspGWI | ACGGA | 1 cut(s) | 414 |
| TspRI | CASTG | 3 cut(s) | 692, 1507, 1825 |
| VpaK11BI | GGWCC | 1 cut(s) | 431 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1397 |
| XapI | RAATTY | 3 cut(s) | 18, 258, 278 |
| XceI | RCATGY | 1 cut(s) | 1772 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 186 |
| XmiI | GTMKAC | 1 cut(s) | 752 |
| XmnI | GAANNNNTTC | 1 cut(s) | 625 |
| XspI | CTAG | 2 cut(s) | 602, 956 |