Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0000300

Physical Location & Seq

Genomic Coordinates Forward (+)

16137923 .. 16141761

Gene Structure 3839 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G007640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGGCTACCACACCTGGAATTCTTACTGACTGGCCATGGACACCTCTTGGGAGCTTTAAGTATGTGGTGTTAGTACCTTGGGTGATTCACAGCACATACTCGTTTATGTTTGGAAAAGAAGGCACAGATCTATTTTACATCCTCATATTTCCATTTCTGTTGTTGAGGATGCTCCACAACCAGATATGGATCTCACTTTCTCGCTATCAAACTGGCATCGAATTCGAACAAGTTGATAGAGAAAAAGATTGGGATGATCAAATCATATTCAACGGAATTCTGTTTTACATAGGCAGGTGGGTCCTGACTAGGTCTCAGATTGAGCTACCAATTTGGAGGGGAAATGGGTTGGTCATGATGATGCTGCTTCATGCTGGTCCGATAGAGTACCTCTACTACTGGTTACACAGAGCACTTCACCACCATTTCCTCTACTCTCGCTACCATTCTCATCACCATTCCTCCATTGTCACTGAGCCAATTACTTCTGTGACTCACCCATTTGCAGAGCACATAATATATTATATGCTCTTCACAGCACCAATGGTGGCAATTCAGTTGATCGGAACAGCTTCCATCACATCCTTTTTTTTATATATTACTTATTTCGACTTCATGAACAACTTGGGACACTGCAACTTCGAGTTCATTCCGAACTGGATCTTCTCTCTGTTTCCTCCTCTTAAGTATCTCATATATACTCCCTCGTACCACTCTTTGCATCACACTCAATTTCGTACCAATTACTCCCTCTTCATGCCAATCTATGACTACATTTATGGTACCATGGACAAGTCTAGTGATACTCTACATGAATCTTCAATCAAGAAAGAGGAATCCCCTGATGTGGTTCATCTAACCCATTTAACAACAACCACGTCCATCTACCGACTCCCATTGGCATTTGCTTCATTGGCCTCTAATCCACACACCTCAACATGGTACTTATGGTTGTTGTGGCCAGTAACATTAGGGTCTATGATGCTAACTCGGATATTTGGCCGTTCATTTGATATTGAGAGACAGCGTTTTGATAAACAAAGACTACAAACTTGGGTTATTCCAAAATACAGTTCGCAGTACTTGCTCTCACGGCAAAATGAAGCTATAAACAGAGCGATTGAGGACGCCATACTTGAAGCTGAGAAAAAGGGTGTCAAAGTTATAAGTTTAGGTCTCTTTAATCAGGGCAAGGGGCTTAATAATTATGGTGAGATATATGTCCAGAGGAATCCACGGCTGAAAATCAAGGTGGTGGATGGGAGTAGCTTAGCTGTGGCTGTCATCCTTAACAGCATTCCAAAAGGCGCTACCCAGGTTCTCCTTACAGGAAACCTCACAAAGGTTGCTCATGCCCTTGCCTTTTCTTTGTGCCAGAGGGATATCCAGGTAGTTACATTACATCAGGCTGAGTATTTGAAGCTCACCAAAGCATTAAATGCTACAGAGGGTAAGGTGGTTCATGACAGAAGCTATGCTCAAAAGTTGCGAAAAGACTGTTACTATCACTGTACTCCAGCCATGAAGATCCCCAAGTCTCTTGAGAATGTTTACTCTTGTGAGAACTGGTTGCCAAGGAGGGTGATGAGTGCGTGGCGTATAGCTGGGATAGTGCATGCATTGGAAGGTTGGAATGACCATGAGTGTGGTTATACCATGTCCGATATTGACAAAGTTTGGCAAGCTACTCTTCGACATGGCTTTCTGCCACTGATCCCCACCGCTGCTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016491 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko00073 ko01110 map00073 map01110

Pfam Domains

Protein Families

FA_hydroxylase Wax2_C

Protein Analysis

577

Amino Acids

67.02

Weight (kDa)

8.73

Isoelectric Point (pI)

31.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FA_hydroxylase	PF04116	124 - 262	9.1e-21	Fatty acid hydroxylase
CER1-like_C	PF12076	440 - 495	3.4e-08	CER1-like, C-terminal domain
CER1-like_C	PF12076	494 - 570	9.7e-41	CER1-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca	FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica	MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica	Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis	pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis	RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata	RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora	Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii	Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa	Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha	Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana	Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1166
AarI	CACCTGC	1 cut(s)	285
Acc36I	ACCTGC	1 cut(s)	285
Acc65I	GGTACC	1 cut(s)	782
AccB1I	GGYRCC	1 cut(s)	782
AciI	CCGC	1 cut(s)	1722
AclWI	GGATC	4 cut(s)	197, 668, 1522, 1708
AcoI	YGGCCR	3 cut(s)	32, 959, 1000
AcsI	RAATTY	3 cut(s)	18, 221, 276
AcyI	GRCGYC	1 cut(s)	1128
AfaI	GTAC	8 cut(s)	75, 389, 710, 739, 784, 944, 1082, 1513
AfiI	CCNNNNNNNGG	3 cut(s)	847, 939, 1342
AflII	CTTAAG	1 cut(s)	683
AgsI	TTSAA	4 cut(s)	271, 822, 1139, 1420
AjiI	CACGTC	1 cut(s)	879
AjnI	CCWGG	3 cut(s)	13, 1314, 1386
AjuI	GAANNNNNNNTTGG	2 cut(s)	1057, 1089
Alw21I	GWGCWC	2 cut(s)	415, 513
Alw26I	GTCTC	4 cut(s)	318, 1015, 1181, 1542
AlwI	GGATC	4 cut(s)	197, 668, 1522, 1708
AoxI	GGCC	4 cut(s)	32, 915, 959, 1000
ApeKI	GCWGC	2 cut(s)	364, 1724
ApoI	RAATTY	3 cut(s)	18, 221, 276
Asp700I	GAANNNNTTC	1 cut(s)	571
Asp718I	GGTACC	1 cut(s)	782
AspLEI	GCGC	1 cut(s)	1310
AspS9I	GGNCC	2 cut(s)	301, 377
AsuHPI	GGTGA	7 cut(s)	94, 410, 446, 488, 1223, 1417, 1594
AsuII	TTCGAA	1 cut(s)	225
AvaII	GGWCC	2 cut(s)	301, 377
BalI	TGGCCA	2 cut(s)	34, 961
BanI	GGYRCC	1 cut(s)	782
Bbv12I	GWGCWC	2 cut(s)	415, 513
BbvI	GCAGC	2 cut(s)	351, 1711
BccI	CCATC	3 cut(s)	584, 890, 1253
BceAI	ACGGC	3 cut(s)	987, 1109, 1253
BcgI	CGANNNNNNTGC	2 cut(s)	205, 239
BciT130I	CCWGG	3 cut(s)	15, 1316, 1388
BclI	TGATCA	1 cut(s)	256
BcoDI	GTCTC	4 cut(s)	318, 1015, 1181, 1542
BfaI	CTAG	2 cut(s)	309, 798
BfmI	CTRYAG	1 cut(s)	1443
BfoI	RGCGCY	1 cut(s)	1311
BfrI	CTTAAG	1 cut(s)	683
BfuAI	ACCTGC	1 cut(s)	285
BglII	AGATCT	1 cut(s)	127
BisI	GCNGC	2 cut(s)	365, 1725
BlpI	GCTNAGC	1 cut(s)	1270
BlsI	GCNGC	2 cut(s)	366, 1726
BmcAI	AGTACT	1 cut(s)	1082
Bme1390I	CCNGG	3 cut(s)	15, 1316, 1388
Bme18I	GGWCC	2 cut(s)	301, 377
BmgBI	CACGTC	1 cut(s)	879
BmgT120I	GGNCC	2 cut(s)	301, 377
BmiI	GGNNCC	2 cut(s)	302, 784
BmrFI	CCNGG	3 cut(s)	15, 1316, 1388
BmsI	GCATC	5 cut(s)	159, 225, 351, 730, 972
BpmI	CTGGAG	1 cut(s)	1500
Bpu1102I	GCTNAGC	1 cut(s)	1270
Bpu14I	TTCGAA	1 cut(s)	225
BpuEI	CTTGAG	1 cut(s)	1562
BsaBI	GATNNNNATC	1 cut(s)	188
BsaHI	GRCGYC	1 cut(s)	1128
BsaI	GGTCTC	2 cut(s)	318, 1181
BsaJI	CCNNGG	6 cut(s)	35, 77, 786, 1235, 1314, 1574
BsaXI	ACNNNNNCTCC	2 cut(s)	446, 476
Bsc4I	CCNNNNNNNGG	3 cut(s)	847, 939, 1342
Bse1I	ACTGG	6 cut(s)	35, 217, 404, 662, 962, 1571
Bse8I	GATNNNNATC	1 cut(s)	188
BseBI	CCWGG	3 cut(s)	15, 1316, 1388
BseDI	CCNNGG	6 cut(s)	35, 77, 786, 1235, 1314, 1574
BseGI	GGATG	6 cut(s)	138, 174, 259, 581, 1264, 1284
BseJI	GATNNNNATC	1 cut(s)	188
BseLI	CCNNNNNNNGG	3 cut(s)	847, 939, 1342
BseMII	CTCAG	4 cut(s)	329, 465, 1134, 1401
BseNI	ACTGG	6 cut(s)	35, 217, 404, 662, 962, 1571
BseRI	GAGGAG	1 cut(s)	669
BseXI	GCAGC	2 cut(s)	351, 1711
BseYI	CCCAGC	1 cut(s)	1604
BshFI	GGCC	4 cut(s)	34, 917, 961, 1002
BshNI	GGYRCC	1 cut(s)	782
BsiHKAI	GWGCWC	2 cut(s)	415, 513
BslFI	GGGAC	1 cut(s)	642
BslI	CCNNNNNNNGG	3 cut(s)	847, 939, 1342
BsmAI	GTCTC	4 cut(s)	318, 1015, 1181, 1542
BsmFI	GGGAC	1 cut(s)	642
BsmI	GAATGC	1 cut(s)	1296
BsnI	GGCC	4 cut(s)	34, 917, 961, 1002
Bso31I	GGTCTC	2 cut(s)	318, 1181
Bsp119I	TTCGAA	1 cut(s)	225
Bsp1286I	GDGCHC	2 cut(s)	415, 513
Bsp143I	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
Bsp1720I	GCTNAGC	1 cut(s)	1270
Bsp19I	CCATGG	2 cut(s)	35, 786
BspACI	CCGC	1 cut(s)	1722
BspANI	GGCC	4 cut(s)	34, 917, 961, 1002
BspCNI	CTCAG	4 cut(s)	328, 466, 1135, 1402
BspHI	TCATGA	3 cut(s)	354, 615, 1462
BspLI	GGNNCC	2 cut(s)	302, 784
BspMI	ACCTGC	1 cut(s)	285
BspPI	GGATC	4 cut(s)	197, 668, 1522, 1708
BspQI	GCTCTTC	1 cut(s)	536
BspT104I	TTCGAA	1 cut(s)	225
BspT107I	GGYRCC	1 cut(s)	782
BspTI	CTTAAG	1 cut(s)	683
BspTNI	GGTCTC	2 cut(s)	318, 1181
BsrI	ACTGG	6 cut(s)	35, 217, 404, 662, 962, 1571
BssECI	CCNNGG	6 cut(s)	35, 77, 786, 1235, 1314, 1574
BssMI	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
BssNI	GRCGYC	1 cut(s)	1128
BssT1I	CCWWGG	4 cut(s)	35, 77, 786, 1574
Bst2UI	CCWGG	3 cut(s)	15, 1316, 1388
Bst4CI	ACNGT	3 cut(s)	1073, 1499, 1511
Bst6I	CTCTTC	3 cut(s)	536, 758, 1695
BstACI	GRCGYC	1 cut(s)	1128
BstAFI	CTTAAG	1 cut(s)	683
BstAPI	GCANNNNNTGC	2 cut(s)	1084, 1439
BstBI	TTCGAA	1 cut(s)	225
BstC8I	GCNNGC	2 cut(s)	1617, 1683
BstDEI	CTNAG	5 cut(s)	315, 474, 1143, 1270, 1410
BstDSI	CCRYGG	3 cut(s)	35, 786, 1235
BstENI	CCTNNNNNAGG	1 cut(s)	1340
BstF5I	GGATG	6 cut(s)	138, 174, 259, 581, 1264, 1284
BstH2I	RGCGCY	1 cut(s)	1311
BstHHI	GCGC	1 cut(s)	1310
BstKTI	GATC	7 cut(s)	130, 192, 259, 564, 663, 1530, 1716
BstMAI	GTCTC	4 cut(s)	318, 1015, 1181, 1542
BstMBI	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
BstMWI	GCNNNNNNNGC	4 cut(s)	914, 1084, 1093, 1439
BstNI	CCWGG	3 cut(s)	15, 1316, 1388
BstNSI	RCATGY	1 cut(s)	1619
BstSCI	CCNGG	3 cut(s)	13, 1314, 1386
BstSFI	CTRYAG	1 cut(s)	1443
BstV1I	GCAGC	2 cut(s)	351, 1711
BstX2I	RGATCY	4 cut(s)	127, 189, 660, 1527
BstXI	CCANNNNNNTGG	1 cut(s)	1646
BstYI	RGATCY	4 cut(s)	127, 189, 660, 1527
BsuRI	GGCC	4 cut(s)	34, 917, 961, 1002
BtgI	CCRYGG	3 cut(s)	35, 786, 1235
BtrI	CACGTC	1 cut(s)	879
BtsCI	GGATG	6 cut(s)	138, 174, 259, 581, 1264, 1284
BtsI	GCAGTG	1 cut(s)	631
BtsIMutI	CAGTG	4 cut(s)	471, 631, 1507, 1709
BveI	ACCTGC	1 cut(s)	285
Cac8I	GCNNGC	2 cut(s)	1617, 1683
CciI	TCATGA	3 cut(s)	354, 615, 1462
CfoI	GCGC	1 cut(s)	1310
Cfr13I	GGNCC	2 cut(s)	301, 377
CseI	GACGC	1 cut(s)	1136
Csp6I	GTAC	8 cut(s)	74, 388, 709, 738, 783, 943, 1081, 1512
CviQI	GTAC	8 cut(s)	74, 388, 709, 738, 783, 943, 1081, 1512
DdeI	CTNAG	5 cut(s)	315, 474, 1143, 1270, 1410
DpnI	GATC	7 cut(s)	129, 191, 258, 563, 662, 1529, 1715
DpnII	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
EaeI	YGGCCR	3 cut(s)	32, 959, 1000
Eam1104I	CTCTTC	3 cut(s)	536, 758, 1695
EarI	CTCTTC	3 cut(s)	536, 758, 1695
Eco130I	CCWWGG	4 cut(s)	35, 77, 786, 1574
Eco31I	GGTCTC	2 cut(s)	318, 1181
Eco32I	GATATC	1 cut(s)	1384
Eco47I	GGWCC	2 cut(s)	301, 377
EcoNI	CCTNNNNNAGG	1 cut(s)	1340
EcoO109I	RGGNCCY	1 cut(s)	301
EcoRI	GAATTC	3 cut(s)	18, 221, 276
EcoRII	CCWGG	3 cut(s)	13, 1314, 1386
EcoRV	GATATC	1 cut(s)	1384
EcoT14I	CCWWGG	4 cut(s)	35, 77, 786, 1574
EcoT22I	ATGCAT	1 cut(s)	1621
ErhI	CCWWGG	4 cut(s)	35, 77, 786, 1574
FalI	AAGNNNNNCTT	2 cut(s)	1674, 1706
FaqI	GGGAC	1 cut(s)	642
FbaI	TGATCA	1 cut(s)	256
Fnu4HI	GCNGC	2 cut(s)	365, 1725
FokI	GGATG	6 cut(s)	125, 181, 266, 568, 1271, 1271
Fsp4HI	GCNGC	2 cut(s)	365, 1725
FspBI	CTAG	2 cut(s)	309, 798
GlaI	GCGC	1 cut(s)	1309
GluI	GCNGC	2 cut(s)	365, 1725
GsaI	CCCAGC	1 cut(s)	1608
GsuI	CTGGAG	1 cut(s)	1500
HaeII	RGCGCY	1 cut(s)	1311
HaeIII	GGCC	4 cut(s)	34, 917, 961, 1002
HgaI	GACGC	1 cut(s)	1136
HhaI	GCGC	1 cut(s)	1310
Hin1I	GRCGYC	1 cut(s)	1128
Hin6I	GCGC	1 cut(s)	1308
HinP1I	GCGC	1 cut(s)	1308
HinfI	GANTC	6 cut(s)	85, 493, 815, 836, 891, 1231
HphI	GGTGA	7 cut(s)	94, 410, 446, 488, 1223, 1417, 1594
Hpy166II	GTNNAC	1 cut(s)	1552
Hpy188I	TCNGA	6 cut(s)	318, 381, 566, 654, 993, 1663
Hpy188III	TCNNGA	7 cut(s)	304, 355, 616, 826, 1225, 1463, 1541
Hpy8I	GTNNAC	1 cut(s)	1552
HpyAV	CCTTC	2 cut(s)	113, 1619
HpyCH4III	ACNGT	3 cut(s)	1073, 1499, 1511
HpyCH4IV	ACGT	1 cut(s)	878
HpyCH4V	TGCA	5 cut(s)	506, 636, 721, 1615, 1619
HpyF10VI	GCNNNNNNNGC	4 cut(s)	914, 1084, 1093, 1439
HpyF3I	CTNAG	5 cut(s)	315, 474, 1143, 1270, 1410
HpySE526I	ACGT	1 cut(s)	878
Hsp92I	GRCGYC	1 cut(s)	1128
HspAI	GCGC	1 cut(s)	1308
KpnI	GGTACC	1 cut(s)	786
Ksp22I	TGATCA	1 cut(s)	256
Kzo9I	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
LguI	GCTCTTC	1 cut(s)	536
LmnI	GCTCC	2 cut(s)	51, 177
Lsp1109I	GCAGC	2 cut(s)	351, 1711
LweI	GCATC	5 cut(s)	159, 225, 351, 730, 972
MaeI	CTAG	2 cut(s)	309, 798
MaeII	ACGT	1 cut(s)	878
MaeIII	GTNAC	6 cut(s)	402, 469, 490, 964, 1393, 1499
MalI	GATC	7 cut(s)	129, 191, 258, 563, 662, 1529, 1715
MboI	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
MboII	GAAGA	6 cut(s)	523, 655, 745, 810, 1537, 1682
MflI	RGATCY	4 cut(s)	127, 189, 660, 1527
MhlI	GDGCHC	2 cut(s)	415, 513
MlsI	TGGCCA	2 cut(s)	34, 961
MluCI	AATT	9 cut(s)	18, 221, 276, 330, 480, 552, 731, 742, 1204
MluNI	TGGCCA	2 cut(s)	34, 961
MlyI	GAGTC	2 cut(s)	487, 885
MmeI	TCCRAC	1 cut(s)	1610
Mox20I	TGGCCA	2 cut(s)	34, 961
Mph1103I	ATGCAT	1 cut(s)	1621
MroXI	GAANNNNTTC	1 cut(s)	571
MscI	TGGCCA	2 cut(s)	34, 961
MseI	TTAA	8 cut(s)	57, 684, 866, 1182, 1200, 1290, 1436, 1732
MslI	CAYNNNNRTG	2 cut(s)	359, 1644
Msp20I	TGGCCA	2 cut(s)	34, 961
MspA1I	CMGCKG	1 cut(s)	1724
MspCI	CTTAAG	1 cut(s)	683
MspR9I	CCNGG	3 cut(s)	15, 1316, 1388
Mva1269I	GAATGC	1 cut(s)	1296
MvaI	CCWGG	3 cut(s)	15, 1316, 1388
MwoI	GCNNNNNNNGC	4 cut(s)	914, 1084, 1093, 1439
NcoI	CCATGG	2 cut(s)	35, 786
NdeII	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
NlaIV	GGNNCC	2 cut(s)	302, 784
NmuCI	GTSAC	2 cut(s)	469, 490
NsiI	ATGCAT	1 cut(s)	1621
NspI	RCATGY	1 cut(s)	1619
NspV	TTCGAA	1 cut(s)	225
PaeI	GCATGC	1 cut(s)	1619
PagI	TCATGA	3 cut(s)	354, 615, 1462
PaqCI	CACCTGC	1 cut(s)	285
PciSI	GCTCTTC	1 cut(s)	536
PctI	GAATGC	1 cut(s)	1296
PdmI	GAANNNNTTC	1 cut(s)	571
PfeI	GAWTC	4 cut(s)	85, 815, 836, 1231
PflFI	GACNNNGTC	1 cut(s)	310
PkrI	GCNGC	2 cut(s)	366, 1726
PleI	GAGTC	2 cut(s)	487, 885
PpsI	GAGTC	2 cut(s)	487, 885
PpuMI	RGGWCCY	1 cut(s)	301
PsiI	TTATAA	1 cut(s)	1166
Psp5II	RGGWCCY	1 cut(s)	301
Psp6I	CCWGG	3 cut(s)	13, 1314, 1386
PspFI	CCCAGC	1 cut(s)	1604
PspGI	CCWGG	3 cut(s)	13, 1314, 1386
PspN4I	GGNNCC	2 cut(s)	302, 784
PspPI	GGNCC	2 cut(s)	301, 377
PspPPI	RGGWCCY	1 cut(s)	301
PsuI	RGATCY	4 cut(s)	127, 189, 660, 1527
PsyI	GACNNNGTC	1 cut(s)	310
RsaI	GTAC	8 cut(s)	75, 389, 710, 739, 784, 944, 1082, 1513
RsaNI	GTAC	8 cut(s)	74, 388, 709, 738, 783, 943, 1081, 1512
RseI	CAYNNNNRTG	2 cut(s)	359, 1644
SapI	GCTCTTC	1 cut(s)	536
SaqAI	TTAA	8 cut(s)	57, 684, 866, 1182, 1200, 1290, 1436, 1732
SatI	GCNGC	2 cut(s)	365, 1725
Sau3AI	GATC	7 cut(s)	127, 189, 256, 561, 660, 1527, 1713
Sau96I	GGNCC	2 cut(s)	301, 377
ScaI	AGTACT	1 cut(s)	1082
SchI	GAGTC	2 cut(s)	487, 885
ScrFI	CCNGG	3 cut(s)	15, 1316, 1388
SduI	GDGCHC	2 cut(s)	415, 513
SfaNI	GCATC	5 cut(s)	159, 225, 351, 730, 972
SfcI	CTRYAG	1 cut(s)	1443
SfuI	TTCGAA	1 cut(s)	225
SinI	GGWCC	2 cut(s)	301, 377
SmiMI	CAYNNNNRTG	2 cut(s)	359, 1644
SmlI	CTYRAG	2 cut(s)	683, 1541
SmoI	CTYRAG	2 cut(s)	683, 1541
SphI	GCATGC	1 cut(s)	1619
Sse9I	AATT	9 cut(s)	18, 221, 276, 330, 480, 552, 731, 742, 1204
SsiI	CCGC	1 cut(s)	1722
SspMI	CTAG	2 cut(s)	309, 798
StyD4I	CCNGG	3 cut(s)	13, 1314, 1386
StyI	CCWWGG	4 cut(s)	35, 77, 786, 1574
TaaI	ACNGT	3 cut(s)	1073, 1499, 1511
TaiI	ACGT	1 cut(s)	881
TaqI	TCGA	5 cut(s)	219, 225, 609, 642, 1693
TasI	AATT	9 cut(s)	18, 221, 276, 330, 480, 552, 731, 742, 1204
TatI	WGTACW	2 cut(s)	1080, 1511
TfiI	GAWTC	4 cut(s)	85, 815, 836, 1231
Tru1I	TTAA	8 cut(s)	57, 684, 866, 1182, 1200, 1290, 1436, 1732
Tru9I	TTAA	8 cut(s)	57, 684, 866, 1182, 1200, 1290, 1436, 1732
TscAI	CASTG	4 cut(s)	478, 638, 1514, 1716
TseFI	GTSAC	2 cut(s)	469, 490
TseI	GCWGC	2 cut(s)	364, 1724
Tsp45I	GTSAC	2 cut(s)	469, 490
TspGWI	ACGGA	1 cut(s)	288
TspRI	CASTG	4 cut(s)	478, 638, 1514, 1716
Tth111I	GACNNNGTC	1 cut(s)	310
Vha464I	CTTAAG	1 cut(s)	683
VpaK11BI	GGWCC	2 cut(s)	301, 377
XagI	CCTNNNNNAGG	1 cut(s)	1340
XapI	RAATTY	3 cut(s)	18, 221, 276
XceI	RCATGY	1 cut(s)	1619
XmnI	GAANNNNTTC	1 cut(s)	571
XspI	CTAG	2 cut(s)	309, 798
ZrmI	AGTACT	1 cut(s)	1082
Zsp2I	ATGCAT	1 cut(s)	1621

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.