Belongs to the sterol desaturase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1619 |
| AccB1I | GGYRCC | 1 cut(s) | 812 |
| AccI | GTMKAC | 1 cut(s) | 728 |
| AciI | CCGC | 3 cut(s) | 228, 959, 1060 |
| AclWI | GGATC | 5 cut(s) | 200, 242, 1087, 1651, 1837 |
| AcsI | RAATTY | 2 cut(s) | 18, 29 |
| AfaI | GTAC | 2 cut(s) | 62, 740 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 535, 1125, 1225, 1375 |
| AgsI | TTSAA | 7 cut(s) | 606, 969, 1216, 1453, 1499, 1515, 1673 |
| AhlI | ACTAGT | 1 cut(s) | 827 |
| AjnI | CCWGG | 3 cut(s) | 13, 77, 1218 |
| AleI | CACNNNNGTG | 1 cut(s) | 1043 |
| Alw21I | GWGCWC | 3 cut(s) | 445, 543, 678 |
| Alw26I | GTCTC | 3 cut(s) | 114, 1334, 1671 |
| AlwI | GGATC | 5 cut(s) | 200, 242, 1087, 1651, 1837 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1626, 1840 |
| AoxI | GGCC | 4 cut(s) | 247, 948, 992, 1405 |
| ApeKI | GCWGC | 3 cut(s) | 225, 327, 1057 |
| ApoI | RAATTY | 2 cut(s) | 18, 29 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 601 |
| AspA2I | CCTAGG | 1 cut(s) | 322 |
| AspLEI | GCGC | 4 cut(s) | 1567, 1620, 1721, 1728 |
| AspS9I | GGNCC | 3 cut(s) | 247, 407, 1405 |
| AsuC2I | CCSGG | 1 cut(s) | 342 |
| AsuHPI | GGTGA | 6 cut(s) | 94, 359, 473, 518, 1235, 1450 |
| AsuII | TTCGAA | 2 cut(s) | 258, 766 |
| AvaII | GGWCC | 1 cut(s) | 407 |
| AvrII | CCTAGG | 1 cut(s) | 322 |
| BanI | GGYRCC | 1 cut(s) | 812 |
| BanII | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| BauI | CACGAG | 1 cut(s) | 1684 |
| BbsI | GAAGAC | 1 cut(s) | 1557 |
| Bbv12I | GWGCWC | 3 cut(s) | 445, 543, 678 |
| BbvCI | CCTCAGC | 1 cut(s) | 1269 |
| BbvI | GCAGC | 3 cut(s) | 237, 339, 1069 |
| BccI | CCATC | 5 cut(s) | 742, 903, 923, 1286, 1529 |
| BciT130I | CCWGG | 3 cut(s) | 15, 79, 1220 |
| BclI | TGATCA | 1 cut(s) | 289 |
| BcnI | CCSGG | 1 cut(s) | 342 |
| BcoDI | GTCTC | 3 cut(s) | 114, 1334, 1671 |
| BcuI | ACTAGT | 1 cut(s) | 827 |
| BfaI | CTAG | 3 cut(s) | 251, 323, 828 |
| BglII | AGATCT | 1 cut(s) | 130 |
| BisI | GCNGC | 4 cut(s) | 226, 328, 959, 1058 |
| BlnI | CCTAGG | 1 cut(s) | 322 |
| BlpI | GCTNAGC | 1 cut(s) | 1303 |
| BlsI | GCNGC | 4 cut(s) | 227, 329, 960, 1059 |
| Bme1390I | CCNGG | 4 cut(s) | 15, 79, 342, 1220 |
| Bme18I | GGWCC | 1 cut(s) | 407 |
| BmgT120I | GGNCC | 3 cut(s) | 247, 407, 1405 |
| BmiI | GGNNCC | 2 cut(s) | 75, 814 |
| BmrFI | CCNGG | 4 cut(s) | 15, 79, 342, 1220 |
| BmrI | ACTGGG | 1 cut(s) | 292 |
| BmsI | GCATC | 4 cut(s) | 162, 760, 895, 1005 |
| BmuI | ACTGGG | 1 cut(s) | 292 |
| BpiI | GAAGAC | 1 cut(s) | 1557 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1252, 1284 |
| BpmI | CTGGAG | 2 cut(s) | 858, 1629 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1269 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1303 |
| Bpu14I | TTCGAA | 2 cut(s) | 258, 766 |
| BpuEI | CTTGAG | 1 cut(s) | 698 |
| BpuMI | CCSGG | 1 cut(s) | 342 |
| BsaAI | YACGTR | 1 cut(s) | 64 |
| BsaJI | CCNNGG | 9 cut(s) | 35, 77, 78, 322, 816, 945, 1124, 1219, 1749 |
| BsaWI | WCCGGW | 1 cut(s) | 409 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 476, 506 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 535, 1125, 1225, 1375 |
| Bse1I | ACTGG | 2 cut(s) | 184, 287 |
| Bse3DI | GCAATG | 1 cut(s) | 1656 |
| BseBI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| BseDI | CCNNGG | 9 cut(s) | 35, 77, 78, 322, 816, 945, 1124, 1219, 1749 |
| BseGI | GGATG | 3 cut(s) | 177, 292, 1447 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 535, 1125, 1225, 1375 |
| BseMI | GCAATG | 1 cut(s) | 1656 |
| BseMII | CTCAG | 2 cut(s) | 495, 1283 |
| BseNI | ACTGG | 2 cut(s) | 184, 287 |
| BseRI | GAGGAG | 2 cut(s) | 384, 1241 |
| BseXI | GCAGC | 3 cut(s) | 237, 339, 1069 |
| BsgI | GTGCAG | 2 cut(s) | 431, 1624 |
| Bsh1285I | CGRYCG | 1 cut(s) | 232 |
| BshFI | GGCC | 4 cut(s) | 249, 950, 994, 1407 |
| BshNI | GGYRCC | 1 cut(s) | 812 |
| BsiEI | CGRYCG | 1 cut(s) | 232 |
| BsiHKAI | GWGCWC | 3 cut(s) | 445, 543, 678 |
| BsiSI | CCGG | 3 cut(s) | 342, 410, 1734 |
| BslFI | GGGAC | 2 cut(s) | 672, 1637 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 535, 1125, 1225, 1375 |
| BsmAI | GTCTC | 3 cut(s) | 114, 1334, 1671 |
| BsmFI | GGGAC | 2 cut(s) | 672, 1637 |
| BsnI | GGCC | 4 cut(s) | 249, 950, 994, 1407 |
| Bsp119I | TTCGAA | 2 cut(s) | 258, 766 |
| Bsp1286I | GDGCHC | 4 cut(s) | 445, 543, 678, 1212 |
| Bsp143I | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1303 |
| Bsp19I | CCATGG | 3 cut(s) | 35, 816, 1124 |
| BspACI | CCGC | 3 cut(s) | 228, 959, 1060 |
| BspANI | GGCC | 4 cut(s) | 249, 950, 994, 1407 |
| BspCNI | CTCAG | 2 cut(s) | 496, 1282 |
| BspLI | GGNNCC | 2 cut(s) | 75, 814 |
| BspPI | GGATC | 5 cut(s) | 200, 242, 1087, 1651, 1837 |
| BspT104I | TTCGAA | 2 cut(s) | 258, 766 |
| BspT107I | GGYRCC | 1 cut(s) | 812 |
| BsrDI | GCAATG | 1 cut(s) | 1656 |
| BsrI | ACTGG | 2 cut(s) | 184, 287 |
| BssECI | CCNNGG | 9 cut(s) | 35, 77, 78, 322, 816, 945, 1124, 1219, 1749 |
| BssMI | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| BssNAI | GTATAC | 1 cut(s) | 729 |
| BssSI | CACGAG | 1 cut(s) | 1684 |
| BssT1I | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| Bst1107I | GTATAC | 1 cut(s) | 729 |
| Bst2BI | CACGAG | 1 cut(s) | 1684 |
| Bst2UI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| Bst4CI | ACNGT | 2 cut(s) | 665, 840 |
| Bst6I | CTCTTC | 1 cut(s) | 788 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1626 |
| BstBAI | YACGTR | 1 cut(s) | 64 |
| BstBI | TTCGAA | 2 cut(s) | 258, 766 |
| BstC8I | GCNNGC | 2 cut(s) | 332, 1746 |
| BstDEI | CTNAG | 5 cut(s) | 504, 954, 1269, 1303, 1479 |
| BstDSI | CCRYGG | 3 cut(s) | 35, 816, 1124 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| BstF5I | GGATG | 3 cut(s) | 177, 292, 1447 |
| BstHHI | GCGC | 4 cut(s) | 1567, 1620, 1721, 1728 |
| BstKTI | GATC | 7 cut(s) | 133, 195, 237, 292, 1095, 1659, 1845 |
| BstMAI | GTCTC | 3 cut(s) | 114, 1334, 1671 |
| BstMBI | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| BstMCI | CGRYCG | 1 cut(s) | 232 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 947, 1564, 1626, 1725 |
| BstNI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| BstNSI | RCATGY | 1 cut(s) | 1748 |
| BstSCI | CCNGG | 4 cut(s) | 13, 77, 340, 1218 |
| BstV1I | GCAGC | 3 cut(s) | 237, 339, 1069 |
| BstV2I | GAAGAC | 1 cut(s) | 1557 |
| BstX2I | RGATCY | 3 cut(s) | 130, 192, 1656 |
| BstYI | RGATCY | 3 cut(s) | 130, 192, 1656 |
| BstZ17I | GTATAC | 1 cut(s) | 729 |
| BsuRI | GGCC | 4 cut(s) | 249, 950, 994, 1407 |
| BtgI | CCRYGG | 3 cut(s) | 35, 816, 1124 |
| BtsCI | GGATG | 3 cut(s) | 177, 292, 1447 |
| BtsI | GCAGTG | 1 cut(s) | 1636 |
| BtsIMutI | CAGTG | 3 cut(s) | 501, 661, 1636 |
| Cac8I | GCNNGC | 2 cut(s) | 332, 1746 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1626, 1840 |
| CfoI | GCGC | 4 cut(s) | 1567, 1620, 1721, 1728 |
| Cfr13I | GGNCC | 3 cut(s) | 247, 407, 1405 |
| Csp6I | GTAC | 2 cut(s) | 61, 739 |
| CviQI | GTAC | 2 cut(s) | 61, 739 |
| DdeI | CTNAG | 5 cut(s) | 504, 954, 1269, 1303, 1479 |
| DpnI | GATC | 7 cut(s) | 132, 194, 236, 291, 1094, 1658, 1844 |
| DpnII | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| Eam1104I | CTCTTC | 1 cut(s) | 788 |
| EarI | CTCTTC | 1 cut(s) | 788 |
| Ecl136II | GAGCTC | 2 cut(s) | 443, 676 |
| Eco130I | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| Eco24I | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| Eco32I | GATATC | 1 cut(s) | 1265 |
| Eco47I | GGWCC | 1 cut(s) | 407 |
| Eco53kI | GAGCTC | 2 cut(s) | 443, 676 |
| EcoICRI | GAGCTC | 2 cut(s) | 443, 676 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| EcoO109I | RGGNCCY | 1 cut(s) | 247 |
| EcoRI | GAATTC | 1 cut(s) | 18 |
| EcoRII | CCWGG | 3 cut(s) | 13, 77, 1218 |
| EcoRV | GATATC | 1 cut(s) | 1265 |
| EcoT14I | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| EcoT38I | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| ErhI | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1152, 1184, 1341, 1373, 1803, 1835 |
| FaqI | GGGAC | 2 cut(s) | 672, 1637 |
| FbaI | TGATCA | 1 cut(s) | 289 |
| FblI | GTMKAC | 1 cut(s) | 728 |
| Fnu4HI | GCNGC | 4 cut(s) | 226, 328, 959, 1058 |
| FokI | GGATG | 3 cut(s) | 184, 299, 1454 |
| FriOI | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| Fsp4HI | GCNGC | 4 cut(s) | 226, 328, 959, 1058 |
| FspBI | CTAG | 3 cut(s) | 251, 323, 828 |
| FspI | TGCGCA | 1 cut(s) | 1619 |
| GlaI | GCGC | 4 cut(s) | 1566, 1619, 1720, 1727 |
| GluI | GCNGC | 4 cut(s) | 226, 328, 959, 1058 |
| GsuI | CTGGAG | 2 cut(s) | 858, 1629 |
| HaeIII | GGCC | 4 cut(s) | 249, 950, 994, 1407 |
| HapII | CCGG | 3 cut(s) | 342, 410, 1734 |
| HhaI | GCGC | 4 cut(s) | 1567, 1620, 1721, 1728 |
| Hin6I | GCGC | 4 cut(s) | 1565, 1618, 1719, 1726 |
| HinP1I | GCGC | 4 cut(s) | 1565, 1618, 1719, 1726 |
| HincII | GTYRAC | 1 cut(s) | 268 |
| HindII | GTYRAC | 1 cut(s) | 268 |
| HinfI | GANTC | 3 cut(s) | 378, 845, 911 |
| HpaII | CCGG | 3 cut(s) | 342, 410, 1734 |
| HphI | GGTGA | 6 cut(s) | 94, 359, 473, 518, 1235, 1450 |
| Hpy166II | GTNNAC | 3 cut(s) | 268, 729, 1681 |
| Hpy188I | TCNGA | 6 cut(s) | 24, 234, 1260, 1792, 1842, 1860 |
| Hpy188III | TCNNGA | 5 cut(s) | 204, 875, 1213, 1496, 1670 |
| Hpy8I | GTNNAC | 3 cut(s) | 268, 729, 1681 |
| HpyAV | CCTTC | 4 cut(s) | 368, 1600, 1701, 1748 |
| HpyCH4III | ACNGT | 2 cut(s) | 665, 840 |
| HpyCH4IV | ACGT | 2 cut(s) | 63, 1430 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 947, 1564, 1626, 1725 |
| HpyF3I | CTNAG | 5 cut(s) | 504, 954, 1269, 1303, 1479 |
| HpySE526I | ACGT | 2 cut(s) | 63, 1430 |
| HspAI | GCGC | 4 cut(s) | 1565, 1618, 1719, 1726 |
| Ksp22I | TGATCA | 1 cut(s) | 289 |
| Kzo9I | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| LmnI | GCTCC | 5 cut(s) | 79, 180, 538, 877, 1228 |
| Lsp1109I | GCAGC | 3 cut(s) | 237, 339, 1069 |
| LweI | GCATC | 4 cut(s) | 162, 760, 895, 1005 |
| MaeI | CTAG | 3 cut(s) | 251, 323, 828 |
| MaeII | ACGT | 2 cut(s) | 63, 1430 |
| MaeIII | GTNAC | 4 cut(s) | 499, 520, 665, 997 |
| MalI | GATC | 7 cut(s) | 132, 194, 236, 291, 1094, 1658, 1844 |
| MboI | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| MboII | GAAGA | 6 cut(s) | 310, 507, 775, 840, 1562, 1666 |
| MfeI | CAATTG | 1 cut(s) | 1389 |
| MflI | RGATCY | 3 cut(s) | 130, 192, 1656 |
| MhlI | GDGCHC | 4 cut(s) | 445, 543, 678, 1212 |
| MmeI | TCCRAC | 2 cut(s) | 212, 1739 |
| MroXI | GAANNNNTTC | 1 cut(s) | 601 |
| MseI | TTAA | 7 cut(s) | 57, 111, 566, 1143, 1170, 1469, 1870 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1043, 1773 |
| MspA1I | CMGCKG | 3 cut(s) | 228, 1060, 1273 |
| MspI | CCGG | 3 cut(s) | 342, 410, 1734 |
| MspR9I | CCNGG | 4 cut(s) | 15, 79, 342, 1220 |
| MunI | CAATTG | 1 cut(s) | 1389 |
| MvaI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 947, 1564, 1626, 1725 |
| NciI | CCSGG | 1 cut(s) | 342 |
| NcoI | CCATGG | 3 cut(s) | 35, 816, 1124 |
| NdeII | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| NlaIV | GGNNCC | 2 cut(s) | 75, 814 |
| NmeAIII | GCCGAG | 1 cut(s) | 1728 |
| NmuCI | GTSAC | 2 cut(s) | 499, 997 |
| NsbI | TGCGCA | 1 cut(s) | 1619 |
| NspI | RCATGY | 1 cut(s) | 1748 |
| NspV | TTCGAA | 2 cut(s) | 258, 766 |
| OliI | CACNNNNGTG | 1 cut(s) | 1043 |
| PaeI | GCATGC | 1 cut(s) | 1748 |
| PasI | CCCWGGG | 1 cut(s) | 78 |
| PdmI | GAANNNNTTC | 1 cut(s) | 601 |
| PfeI | GAWTC | 3 cut(s) | 378, 845, 911 |
| PfoI | TCCNGGA | 1 cut(s) | 13 |
| PkrI | GCNGC | 4 cut(s) | 227, 329, 960, 1059 |
| Ppu21I | YACGTR | 1 cut(s) | 64 |
| Psp124BI | GAGCTC | 2 cut(s) | 445, 678 |
| Psp6I | CCWGG | 3 cut(s) | 13, 77, 1218 |
| PspGI | CCWGG | 3 cut(s) | 13, 77, 1218 |
| PspN4I | GGNNCC | 2 cut(s) | 75, 814 |
| PspPI | GGNCC | 3 cut(s) | 247, 407, 1405 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1626, 1840 |
| PsuI | RGATCY | 3 cut(s) | 130, 192, 1656 |
| PvuII | CAGCTG | 1 cut(s) | 1273 |
| RsaI | GTAC | 2 cut(s) | 62, 740 |
| RsaNI | GTAC | 2 cut(s) | 61, 739 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1043, 1773 |
| SacI | GAGCTC | 2 cut(s) | 445, 678 |
| SaqAI | TTAA | 7 cut(s) | 57, 111, 566, 1143, 1170, 1469, 1870 |
| SatI | GCNGC | 4 cut(s) | 226, 328, 959, 1058 |
| Sau3AI | GATC | 7 cut(s) | 130, 192, 234, 289, 1092, 1656, 1842 |
| Sau96I | GGNCC | 3 cut(s) | 247, 407, 1405 |
| ScrFI | CCNGG | 4 cut(s) | 15, 79, 342, 1220 |
| SduI | GDGCHC | 4 cut(s) | 445, 543, 678, 1212 |
| SfaNI | GCATC | 4 cut(s) | 162, 760, 895, 1005 |
| SfuI | TTCGAA | 2 cut(s) | 258, 766 |
| SinI | GGWCC | 1 cut(s) | 407 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1043, 1773 |
| SmlI | CTYRAG | 1 cut(s) | 713 |
| SmoI | CTYRAG | 1 cut(s) | 713 |
| SpeI | ACTAGT | 1 cut(s) | 827 |
| SphI | GCATGC | 1 cut(s) | 1748 |
| SsiI | CCGC | 3 cut(s) | 228, 959, 1060 |
| SspI | AATATT | 1 cut(s) | 1868 |
| SspMI | CTAG | 3 cut(s) | 251, 323, 828 |
| SstI | GAGCTC | 2 cut(s) | 445, 678 |
| StyD4I | CCNGG | 4 cut(s) | 13, 77, 340, 1218 |
| StyI | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| TaaI | ACNGT | 2 cut(s) | 665, 840 |
| TaiI | ACGT | 2 cut(s) | 66, 1433 |
| TaqI | TCGA | 7 cut(s) | 205, 240, 258, 672, 766, 1010, 1822 |
| TauI | GCSGC | 1 cut(s) | 961 |
| TfiI | GAWTC | 3 cut(s) | 378, 845, 911 |
| Tru1I | TTAA | 7 cut(s) | 57, 111, 566, 1143, 1170, 1469, 1870 |
| Tru9I | TTAA | 7 cut(s) | 57, 111, 566, 1143, 1170, 1469, 1870 |
| TscAI | CASTG | 3 cut(s) | 508, 668, 1643 |
| TseFI | GTSAC | 2 cut(s) | 499, 997 |
| TseI | GCWGC | 3 cut(s) | 225, 327, 1057 |
| Tsp45I | GTSAC | 2 cut(s) | 499, 997 |
| TspGWI | ACGGA | 1 cut(s) | 132 |
| TspRI | CASTG | 3 cut(s) | 508, 668, 1643 |
| VpaK11BI | GGWCC | 1 cut(s) | 407 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| XapI | RAATTY | 2 cut(s) | 18, 29 |
| XceI | RCATGY | 1 cut(s) | 1748 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 580 |
| XmaJI | CCTAGG | 1 cut(s) | 322 |
| XmiI | GTMKAC | 1 cut(s) | 728 |
| XmnI | GAANNNNTTC | 1 cut(s) | 601 |
| XspI | CTAG | 3 cut(s) | 251, 323, 828 |