pycom07g06930

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000300
Physical Location & Seq
Reverse (-)
6273555 .. 6276275
2721 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06930.1

Sequence Viewer

Length: 1146 bp
ATGCACCGTAGGTACCATTCTTTGCACCACACGCATTTCAGGACCAATTACTGTCTCTTCATGCCAATCTACGACTACGTATATGGCACCATGGACAAATCAAGCGATTCACTCTATGATACTTCACTCAAGAGGGAGGAGGAATCGCCAGATGTCCTCCATCTAACCCATCTAACCACACCCGAGTCCATCTTTCATCTACCCCTAGGATTTGCTGCCTTAGCCTCTAGGCCCTACACAAGGACGTGGTACTTGTGGCTGATGTGGCCTGTGACATTGTGGTCTATGATGATAACTTGGATCCACGGCCGTACATTTGTTGTCGACAGGCACCGCTTTGACAAGCTCAGATTACAGACTTGGGCCATTCCCAAGTACAGTTTGCAATACTTTATGCAATGGCAAAATGAAGCTATCAATGGTTTGATTGAGGACGCCATACTTGAAGCAGAGGAAAAGGGTGTCAAGGTTTTAAGCCTAGGTCTCTTGAACCAGGGTGAGGAGTTGAATAGAAATGGCGGTCTCTACGTTCAAAGGCATCCACATCTCAAAATCAAGGTGGTGGATGGAAGTAGCTTGGCTGTGGCAGTAATCTTAGACAGCATTCCAAAAGGGACAACCCAAGTTGTTCTTAGAGGCAAACTCACAAAGGTTGCTTATGCCATTGCCTTTGCTTTGAGCCAGAAGGGTGTCCAGGTAGGTGTGTTATACCAGGCTGAGTATTTGAAGCTCACCAAATCACTAGGCACTGAAAGTAATTTGGTTGTTGCAAAAAGCAATGCCCAAAAGATATGGTTAGTGGGTGATGGAATGATCGAGAAAGAACAGATGAGTGCACCAAGAGGAACATTATTTGTTCCCTTCTCACAATTCCCACCCAAAAAATTGCGCAAGGACTGCTTCTATCACTACACTCCAGCAATGAAGACTCCCACATCTCTTGAGAATGTTCACGCTTGCGAGAACTGGTTGCCAAGAAGGGTGATGAGTGCATGGCGAGTAGCAGGAATAGTGCATGCCTTGGAAGGTTGGAAGGAGCATGATTGTGGTTACACCATGTCCAATATCGACAAAGTTTGGCAAGCAAGTCTTGTGCATGGGTTTCAGCCTCTGACGATCAATACCATTCACACCAAAAATCAGTGA

Protein Analysis

382

Amino Acids

43.82

Weight (kDa)

9.19

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CER1-like_C PF12076 209 - 370 2.3e-72 CER1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 280
Acc16I TGCGCA 1 cut(s) 890
Acc65I GGTACC 1 cut(s) 12
AccB1I GGYRCC 3 cut(s) 12, 86, 330
AccI GTMKAC 1 cut(s) 324
AciI CCGC 2 cut(s) 334, 519
AclWI GGATC 2 cut(s) 295, 308
AcoI YGGCCR 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 435
AfaI GTAC 4 cut(s) 14, 251, 313, 377
AfiI CCNNNNNNNGG 2 cut(s) 240, 499
AgsI TTSAA 5 cut(s) 446, 490, 508, 533, 727
AjiI CACGTC 1 cut(s) 246
AjnI CCWGG 3 cut(s) 492, 693, 711
AluBI AGCT 4 cut(s) 346, 413, 576, 730
AluI AGCT 4 cut(s) 346, 413, 576, 730
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 3 cut(s) 59, 488, 527
Alw44I GTGCAC 1 cut(s) 834
AlwI GGATC 2 cut(s) 295, 308
AlwNI CAGNNNCTG 1 cut(s) 1111
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 4 cut(s) 230, 266, 307, 363
ApaLI GTGCAC 1 cut(s) 834
ApeKI GCWGC 1 cut(s) 215
Asp718I GGTACC 1 cut(s) 12
AspA2I CCTAGG 2 cut(s) 205, 478
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 3 cut(s) 42, 231, 363
AsuHPI GGTGA 4 cut(s) 509, 724, 815, 994
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 42
AvrII CCTAGG 2 cut(s) 205, 478
BaeGI GKGCMC 1 cut(s) 838
BamHI GGATCC 1 cut(s) 300
BanI GGYRCC 3 cut(s) 12, 86, 330
BbsI GAAGAC 1 cut(s) 932
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 1 cut(s) 202
BccI CCATC 5 cut(s) 168, 177, 197, 560, 800
BceAI ACGGC 2 cut(s) 294, 322
BciT130I CCWGG 3 cut(s) 494, 695, 713
BcoDI GTCTC 3 cut(s) 59, 488, 527
BfaI CTAG 4 cut(s) 206, 228, 479, 743
BisI GCNGC 1 cut(s) 216
BlnI CCTAGG 2 cut(s) 205, 478
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 3 cut(s) 494, 695, 713
Bme18I GGWCC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 246
BmgT120I GGNCC 3 cut(s) 42, 231, 363
BmiI GGNNCC 4 cut(s) 14, 88, 302, 332
BmrFI CCNGG 3 cut(s) 494, 695, 713
BmsI GCATC 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 932
BplI GAGNNNNNCTC 2 cut(s) 627, 659
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 1 cut(s) 220
BpuEI CTTGAG 2 cut(s) 113, 962
BsaAI YACGTR 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 2 cut(s) 488, 527
BsaJI CCNNGG 6 cut(s) 90, 205, 304, 478, 493, 1020
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 499
Bse1I ACTGG 1 cut(s) 971
Bse3DI GCAATG 4 cut(s) 404, 663, 784, 927
BseBI CCWGG 3 cut(s) 494, 695, 713
BseDI CCNNGG 6 cut(s) 90, 205, 304, 478, 493, 1020
BseGI GGATG 2 cut(s) 538, 571
BseLI CCNNNNNNNGG 2 cut(s) 240, 499
BseMI GCAATG 4 cut(s) 404, 663, 784, 927
BseMII CTCAG 2 cut(s) 361, 708
BseNI ACTGG 1 cut(s) 971
BseRI GAGGAG 2 cut(s) 152, 515
BseSI GKGCMC 1 cut(s) 838
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 202
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 4 cut(s) 232, 268, 309, 365
BshNI GGYRCC 3 cut(s) 12, 86, 330
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 838
BsiHKCI CYCGRG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 2 cut(s) 240, 499
BsmAI GTCTC 3 cut(s) 59, 488, 527
BsmFI GGGAC 1 cut(s) 628
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 4 cut(s) 232, 268, 309, 365
Bso31I GGTCTC 2 cut(s) 488, 527
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 838
Bsp143I GATC 3 cut(s) 300, 813, 1116
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 2 cut(s) 334, 519
BspANI GGCC 4 cut(s) 232, 268, 309, 365
BspCNI CTCAG 2 cut(s) 360, 709
BspLI GGNNCC 4 cut(s) 14, 88, 302, 332
BspPI GGATC 2 cut(s) 295, 308
BspT107I GGYRCC 3 cut(s) 12, 86, 330
BspTNI GGTCTC 2 cut(s) 488, 527
BsrDI GCAATG 4 cut(s) 404, 663, 784, 927
BsrI ACTGG 1 cut(s) 971
BssECI CCNNGG 6 cut(s) 90, 205, 304, 478, 493, 1020
BssMI GATC 3 cut(s) 300, 813, 1116
BssNI GRCGYC 1 cut(s) 435
BssT1I CCWWGG 4 cut(s) 90, 205, 478, 1020
Bst2UI CCWGG 3 cut(s) 494, 695, 713
Bst4CI ACNGT 3 cut(s) 8, 53, 380
Bst6I CTCTTC 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 435
BstAPI GCANNNNNTGC 1 cut(s) 897
BstBAI YACGTR 1 cut(s) 79
BstC8I GCNNGC 3 cut(s) 958, 1017, 1083
BstDEI CTNAG 5 cut(s) 220, 347, 595, 632, 717
BstDSI CCRYGG 2 cut(s) 90, 304
BstENI CCTNNNNNAGG 1 cut(s) 238
BstF5I GGATG 2 cut(s) 538, 571
BstHHI GCGC 1 cut(s) 891
BstKTI GATC 3 cut(s) 303, 816, 1119
BstMAI GTCTC 3 cut(s) 59, 488, 527
BstMBI GATC 3 cut(s) 300, 813, 1116
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 4 cut(s) 31, 221, 265, 897
BstNI CCWGG 3 cut(s) 494, 695, 713
BstNSI RCATGY 1 cut(s) 1019
BstSCI CCNGG 3 cut(s) 492, 693, 711
BstSLI GKGCMC 1 cut(s) 838
BstSNI TACGTA 1 cut(s) 79
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 1 cut(s) 932
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 4 cut(s) 232, 268, 309, 365
BtgI CCRYGG 2 cut(s) 90, 304
BtrI CACGTC 1 cut(s) 246
BtsCI GGATG 2 cut(s) 538, 571
BtsIMutI CAGTG 1 cut(s) 747
Cac8I GCNNGC 3 cut(s) 958, 1017, 1083
CaiI CAGNNNCTG 1 cut(s) 1111
CfoI GCGC 1 cut(s) 891
Cfr13I GGNCC 3 cut(s) 42, 231, 363
CseI GACGC 1 cut(s) 443
Csp6I GTAC 4 cut(s) 13, 250, 312, 376
CspCI CAANNNNNGTGG 2 cut(s) 922, 957
CviAII CATG 7 cut(s) 61, 91, 993, 1016, 1040, 1057, 1097
CviQI GTAC 4 cut(s) 13, 250, 312, 376
DdeI CTNAG 5 cut(s) 220, 347, 595, 632, 717
DpnI GATC 3 cut(s) 302, 815, 1118
DpnII GATC 3 cut(s) 300, 813, 1116
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 307
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EclXI CGGCCG 1 cut(s) 307
Eco105I TACGTA 1 cut(s) 79
Eco130I CCWWGG 4 cut(s) 90, 205, 478, 1020
Eco31I GGTCTC 2 cut(s) 488, 527
Eco47I GGWCC 1 cut(s) 42
Eco52I CGGCCG 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 182
EcoNI CCTNNNNNAGG 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 231
EcoRII CCWGG 3 cut(s) 492, 693, 711
EcoT14I CCWWGG 4 cut(s) 90, 205, 478, 1020
ErhI CCWWGG 4 cut(s) 90, 205, 478, 1020
FaeI CATG 7 cut(s) 64, 94, 996, 1019, 1043, 1060, 1100
FalI AAGNNNNNCTT 6 cut(s) 615, 647, 884, 916, 1074, 1106
FaqI GGGAC 1 cut(s) 628
FatI CATG 7 cut(s) 60, 90, 992, 1015, 1039, 1056, 1096
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 525, 578
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 4 cut(s) 206, 228, 479, 743
FspI TGCGCA 1 cut(s) 890
GlaI GCGC 1 cut(s) 890
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 4 cut(s) 232, 268, 309, 365
HgaI GACGC 1 cut(s) 443
HhaI GCGC 1 cut(s) 891
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 7 cut(s) 64, 94, 996, 1019, 1043, 1060, 1100
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HinfI GANTC 4 cut(s) 107, 143, 185, 928
HphI GGTGA 4 cut(s) 509, 724, 815, 994
Hpy166II GTNNAC 3 cut(s) 325, 836, 952
Hpy188I TCNGA 2 cut(s) 350, 1113
Hpy188III TCNNGA 5 cut(s) 40, 130, 487, 817, 941
Hpy8I GTNNAC 3 cut(s) 325, 836, 952
HpyAV CCTTC 5 cut(s) 679, 871, 972, 1019, 1027
HpyCH4III ACNGT 3 cut(s) 8, 53, 380
HpyCH4IV ACGT 3 cut(s) 78, 245, 528
HpyCH4V TGCA 9 cut(s) 4, 25, 385, 397, 770, 836, 992, 1015, 1096
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 221, 265, 897
HpyF3I CTNAG 5 cut(s) 220, 347, 595, 632, 717
HpySE526I ACGT 3 cut(s) 78, 245, 528
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 7 cut(s) 64, 94, 996, 1019, 1043, 1060, 1100
HspAI GCGC 1 cut(s) 889
KpnI GGTACC 1 cut(s) 16
Kzo9I GATC 3 cut(s) 300, 813, 1116
LmnI GCTCC 1 cut(s) 1036
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 1 cut(s) 547
MaeI CTAG 4 cut(s) 206, 228, 479, 743
MaeII ACGT 3 cut(s) 78, 245, 528
MaeIII GTNAC 2 cut(s) 271, 1049
MalI GATC 3 cut(s) 302, 815, 1118
MboI GATC 3 cut(s) 300, 813, 1116
MboII GAAGA 2 cut(s) 49, 937
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 1 cut(s) 838
MluCI AATT 4 cut(s) 46, 757, 869, 884
MlyI GAGTC 2 cut(s) 194, 922
MmeI TCCRAC 1 cut(s) 1010
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 1044
MspR9I CCNGG 3 cut(s) 494, 695, 713
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 3 cut(s) 494, 695, 713
MwoI GCNNNNNNNGC 4 cut(s) 31, 221, 265, 897
NcoI CCATGG 1 cut(s) 90
NdeII GATC 3 cut(s) 300, 813, 1116
NlaIII CATG 7 cut(s) 64, 94, 996, 1019, 1043, 1060, 1100
NlaIV GGNNCC 4 cut(s) 14, 88, 302, 332
NmuCI GTSAC 1 cut(s) 271
NsbI TGCGCA 1 cut(s) 890
NspI RCATGY 1 cut(s) 1019
PaeI GCATGC 1 cut(s) 1019
PctI GAATGC 1 cut(s) 603
PfeI GAWTC 2 cut(s) 107, 143
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 2 cut(s) 193, 922
PpsI GAGTC 2 cut(s) 193, 922
Ppu21I YACGTR 1 cut(s) 79
Psp6I CCWGG 3 cut(s) 492, 693, 711
PspGI CCWGG 3 cut(s) 492, 693, 711
PspN4I GGNNCC 4 cut(s) 14, 88, 302, 332
PspPI GGNCC 3 cut(s) 42, 231, 363
PstNI CAGNNNCTG 1 cut(s) 1111
PsuI RGATCY 1 cut(s) 300
RsaI GTAC 4 cut(s) 14, 251, 313, 377
RsaNI GTAC 4 cut(s) 13, 250, 312, 376
RseI CAYNNNNRTG 1 cut(s) 1044
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 3 cut(s) 300, 813, 1116
Sau96I GGNCC 3 cut(s) 42, 231, 363
SchI GAGTC 2 cut(s) 194, 922
ScrFI CCNGG 3 cut(s) 494, 695, 713
SduI GDGCHC 1 cut(s) 838
SfaNI GCATC 1 cut(s) 547
SinI GGWCC 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 1044
SmlI CTYRAG 2 cut(s) 128, 941
SmoI CTYRAG 2 cut(s) 128, 941
SnaBI TACGTA 1 cut(s) 79
SphI GCATGC 1 cut(s) 1019
Sse9I AATT 4 cut(s) 46, 757, 869, 884
SsiI CCGC 2 cut(s) 334, 519
SspMI CTAG 4 cut(s) 206, 228, 479, 743
StyD4I CCNGG 3 cut(s) 492, 693, 711
StyI CCWWGG 4 cut(s) 90, 205, 478, 1020
TaaI ACNGT 3 cut(s) 8, 53, 380
TaiI ACGT 3 cut(s) 81, 248, 531
TaqI TCGA 3 cut(s) 324, 816, 1068
TasI AATT 4 cut(s) 46, 757, 869, 884
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 2 cut(s) 107, 143
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 4 cut(s) 49, 185, 423, 938
TspRI CASTG 1 cut(s) 754
VneI GTGCAC 1 cut(s) 834
VpaK11BI GGWCC 1 cut(s) 42
XagI CCTNNNNNAGG 1 cut(s) 238
XceI RCATGY 1 cut(s) 1019
XmaJI CCTAGG 2 cut(s) 205, 478
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 4 cut(s) 206, 228, 479, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.