FvH4_7g08560

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000300
Physical Location & Seq
Forward (+)
8412892 .. 8417635
4744 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08560.t3

Sequence Viewer

Length: 1878 bp
ATGGCTTCCACTCCTGGTATTCTCACAGACTGGCCATGGACTCCTCTCGGAAGCTTCAAGTACGTGGTTTTGGCTCCTTGGGTGGTTCACAGCACATACTCGTTCATGGTTAACGAAGGACCTGATAGAGATCTGTCTTACTTTCTCATACTTCCGCTCATGCTATGGAGGATGCTCCACAACCAGCTATGGATCTCTCTTTCTCGATATCGAACCGCCAAAGGCAAAGGCCGGATCGTCGACAAGGGCCTCGAATTCGAGCAAGTCGACAGAGAAAGAAACTGGGATGACCAGATAATATTCAACGGAATATTGTTCTACCTAGGCAGCAGGCACCTGCCCGGCGGTAGCAACTTACCGATGTGGAAGGCAGACGGGTTGGTCATAACGTTTCTGCTTCACGCCGGTCCGGTGGAGTTCCTCTACTACTGGCTTCACAGAGCTCTTCACCACCATTTTCTCTACTCTCGCTACCATTCCCACCACCATTCCTCCATCGTCACCGAGCCTATTTCTTCTGTGATTCACCCTTTTGCGGAACACATAGCATATTTCATCCTCTTCGCAATTCCACAGGTGACAATAGCGTTGACGGGGACAGGTTCAGTCGCATCATATTTGATTTATCTTACATATATCGACCTAATGAACAACATGGGGCACTGCAATTTCGAGCTCATTCCCAACTGGATATTTTCTATTTTTCCTCCTCTTAAGTACTTCATGTACACTCCCTCGTTTCACTCACTTCATCACACACAATTCCGAACAAATTACTCGCTCTTTATGCCGATTTACGACTACATATACGGCACTACGGACAAGTCTACCGATTCTCTGTATGAATCTTCACTGAAAAGAGAAGAGGAATCGCCGGATGTGCTGCATCTGACCCATTTAACAACCCCTGAATCTATCTATCAACTTCCGGTTGGGTTTGCTTCCTTGGCCTCTAAGCCACACACCTCAACATGGTACTTATGGTTGATGTGGCCTGTCACACTGTGGTCTATGCTGCTCACTTGGATCTATGGCCGCACTTTCGTGGTCGAGAGGCAGCGGTTTGAGAAGCTTAAATTGCAAACTTGGGCTATACCTAAATACAGTTTGCAGTACTTCTTGGAGTGGCAAAACGAAGCTATCAACGGCCTGATTGAGGGAGCTATACTTGAAGCCGAGGAAAAGGGTGTCAAAGTTTTAACTTTAGGTCTCTTGAATCAGAGTGAGGAGCTGAATAGATACGGCGGCCTATATGTTCACAGGAATCCGCAGCTGAAAATCAAGGTGGTGGATGGAAGTAGCTTAGCTGTGGCTGTCATTCTTAACAGCATTCCGAAAGGGACAACAGAAGTTCTTCTTAGAGGCAACCTCACAAAGGTCGCCTACGCCCTTGCCTTTTCTTTGTGCCAGAAGGGAATCCAGGTTGCTACGCTACACCAGTCTGATTATTTGAAGCTCACCAAATCATTCAGTGCTACTGAGAGTAAGTTGGTTCTTGCAAAGAGCTATGCTGCAAAGATCTGGTTAGTGGGAGATGGATTGAGCAAGGAAGAACAGTTGAATGCACCAAAGGGAACTATATTTGTTCCCTTCTCCCAACTTCCTCCCAAAAAATTGCGCAAAGACTGCTACTACCACTGTACCCCAGCCATGAAAACTCCTACATCCCTTGAGAACATTTACTCTTGTGAGAACTGGTTGCCAAGAAGAGTGATGAGTGTGTGGCGCATTGCCGGAATAGTGCATGCCTTGGAAGGTTGGAATAAGCACGAGTGCGGTTACACCATGTCGGACATTAACAAAGTTTGGCAAGCAACTCTTCGACATGGCTTCCAGCCTCTTACCACCACAGTCACTACTCAGACCAAAAATGACTGA

Protein Analysis

626

Amino Acids

71.97

Weight (kDa)

8.69

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 98 - 237 3.6e-21 Fatty acid hydroxylase
CER1-like_C PF12076 416 - 578 1.2e-72 CER1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 345
AasI GACNNNNNNGTC 1 cut(s) 380
Acc16I TGCGCA 1 cut(s) 1619
Acc36I ACCTGC 1 cut(s) 345
AccB1I GGYRCC 1 cut(s) 333
AccBSI CCGCTC 1 cut(s) 157
AccI GTMKAC 3 cut(s) 240, 267, 827
AciI CCGC 9 cut(s) 155, 216, 345, 536, 1036, 1060, 1245, 1268, 1776
AclI AACGTT 1 cut(s) 389
AclWI GGATC 3 cut(s) 200, 242, 1034
AcoI YGGCCR 2 cut(s) 32, 1033
AcsI RAATTY 1 cut(s) 254
AdeI CACNNNGTG 1 cut(s) 1005
AfaI GTAC 6 cut(s) 62, 719, 728, 977, 1115, 1642
AfiI CCNNNNNNNGG 4 cut(s) 535, 972, 1156, 1375
AflII CTTAAG 1 cut(s) 713
AgsI TTSAA 6 cut(s) 58, 304, 1172, 1216, 1453, 1561
AjnI CCWGG 2 cut(s) 13, 1419
AleI CACNNNNGTG 1 cut(s) 1043
Alw21I GWGCWC 2 cut(s) 445, 678
Alw26I GTCTC 1 cut(s) 1214
AlwI GGATC 3 cut(s) 200, 242, 1034
AoxI GGCC 8 cut(s) 32, 229, 247, 948, 992, 1033, 1147, 1246
ApeKI GCWGC 6 cut(s) 327, 883, 1015, 1057, 1270, 1511
ApoI RAATTY 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 1353
AspA2I CCTAGG 1 cut(s) 322
AspLEI GCGC 2 cut(s) 1620, 1728
AspS9I GGNCC 3 cut(s) 119, 247, 407
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 5 cut(s) 440, 493, 518, 589, 1450
AvaII GGWCC 2 cut(s) 119, 407
AvrII CCTAGG 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 663
BaeI ACNNNNGTAYC 2 cut(s) 1624, 1657
BalI TGGCCA 1 cut(s) 34
BanI GGYRCC 1 cut(s) 333
BanII GRGCYC 2 cut(s) 445, 678
BauI CACGAG 1 cut(s) 1769
Bbv12I GWGCWC 2 cut(s) 445, 678
BbvI GCAGC 6 cut(s) 339, 870, 1002, 1069, 1282, 1498
BccI CCATC 3 cut(s) 503, 1286, 1529
BceAI ACGGC 3 cut(s) 826, 1162, 1258
BciT130I CCWGG 2 cut(s) 15, 1421
BcnI CCSGG 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 1214
BfaI CTAG 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 713
BfuAI ACCTGC 1 cut(s) 345
BglII AGATCT 2 cut(s) 130, 1518
BisI GCNGC 8 cut(s) 328, 884, 1016, 1036, 1058, 1246, 1271, 1512
BlnI CCTAGG 1 cut(s) 322
BlpI GCTNAGC 1 cut(s) 1303
BlsI GCNGC 8 cut(s) 329, 885, 1017, 1037, 1059, 1247, 1272, 1513
BmcAI AGTACT 2 cut(s) 719, 1115
Bme1390I CCNGG 3 cut(s) 15, 342, 1421
Bme18I GGWCC 2 cut(s) 119, 407
BmgT120I GGNCC 3 cut(s) 119, 247, 407
BmiI GGNNCC 2 cut(s) 75, 335
BmrFI CCNGG 3 cut(s) 15, 342, 1421
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 3 cut(s) 162, 620, 895
BmuI ACTGGG 1 cut(s) 292
BplI GAGNNNNNCTC 2 cut(s) 1353, 1385
Bpu1102I GCTNAGC 1 cut(s) 1303
BpuEI CTTGAG 1 cut(s) 1691
BpuMI CCSGG 1 cut(s) 342
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 1214
BsaJI CCNNGG 6 cut(s) 35, 77, 322, 945, 1176, 1749
BsaWI WCCGGW 2 cut(s) 409, 928
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 4 cut(s) 535, 972, 1156, 1375
Bse118I RCCGGY 1 cut(s) 404
Bse1I ACTGG 6 cut(s) 35, 287, 434, 692, 1438, 1700
Bse3DI GCAATG 1 cut(s) 1728
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 2 cut(s) 15, 1421
BseDI CCNNGG 6 cut(s) 35, 77, 322, 945, 1176, 1749
BseGI GGATG 6 cut(s) 177, 292, 555, 883, 1297, 1664
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 4 cut(s) 535, 972, 1156, 1375
BseMI GCAATG 1 cut(s) 1728
BseMII CTCAG 2 cut(s) 1470, 1874
BseNI ACTGG 6 cut(s) 35, 287, 434, 692, 1438, 1700
BseRI GAGGAG 3 cut(s) 33, 699, 1241
BseSI GKGCMC 1 cut(s) 663
BseXI GCAGC 6 cut(s) 339, 870, 1002, 1069, 1282, 1498
BseYI CCCAGC 1 cut(s) 1645
BshFI GGCC 8 cut(s) 34, 231, 249, 950, 994, 1035, 1149, 1248
BshNI GGYRCC 1 cut(s) 333
BsiHKAI GWGCWC 2 cut(s) 445, 678
BsiSI CCGG 7 cut(s) 232, 342, 405, 410, 875, 929, 1734
BslFI GGGAC 2 cut(s) 610, 1354
BslI CCNNNNNNNGG 4 cut(s) 535, 972, 1156, 1375
BsmAI GTCTC 1 cut(s) 1214
BsmFI GGGAC 2 cut(s) 610, 1354
BsmI GAATGC 2 cut(s) 1329, 1567
BsnI GGCC 8 cut(s) 34, 231, 249, 950, 994, 1035, 1149, 1248
Bso31I GGTCTC 1 cut(s) 1214
Bsp1286I GDGCHC 3 cut(s) 445, 663, 678
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 5 cut(s) 130, 192, 234, 1026, 1518
Bsp1720I GCTNAGC 1 cut(s) 1303
Bsp19I CCATGG 1 cut(s) 35
BspACI CCGC 9 cut(s) 155, 216, 345, 536, 1036, 1060, 1245, 1268, 1776
BspANI GGCC 8 cut(s) 34, 231, 249, 950, 994, 1035, 1149, 1248
BspCNI CTCAG 2 cut(s) 1471, 1873
BspLI GGNNCC 2 cut(s) 75, 335
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 3 cut(s) 200, 242, 1034
BspQI GCTCTTC 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 333
BspTI CTTAAG 1 cut(s) 713
BspTNI GGTCTC 1 cut(s) 1214
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 1728
BsrFI RCCGGY 1 cut(s) 404
BsrGI TGTACA 1 cut(s) 726
BsrI ACTGG 6 cut(s) 35, 287, 434, 692, 1438, 1700
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 6 cut(s) 35, 77, 322, 945, 1176, 1749
BssMI GATC 5 cut(s) 130, 192, 234, 1026, 1518
BssSI CACGAG 1 cut(s) 1769
BssT1I CCWWGG 5 cut(s) 35, 77, 322, 945, 1749
Bst2BI CACGAG 1 cut(s) 1769
Bst2UI CCWGG 2 cut(s) 15, 1421
Bst4CI ACNGT 5 cut(s) 1005, 1106, 1557, 1640, 1852
Bst6I CTCTTC 5 cut(s) 450, 566, 858, 1702, 1824
BstAFI CTTAAG 1 cut(s) 713
BstAPI GCANNNNNTGC 1 cut(s) 1626
BstAUI TGTACA 1 cut(s) 726
BstBAI YACGTR 1 cut(s) 64
BstC8I GCNNGC 3 cut(s) 332, 1746, 1812
BstDEI CTNAG 5 cut(s) 954, 1303, 1358, 1479, 1860
BstDSI CCRYGG 1 cut(s) 35
BstENI CCTNNNNNAGG 2 cut(s) 1154, 1373
BstF5I GGATG 6 cut(s) 177, 292, 555, 883, 1297, 1664
BstHHI GCGC 2 cut(s) 1620, 1728
BstKTI GATC 5 cut(s) 133, 195, 237, 1029, 1521
BstMAI GTCTC 1 cut(s) 1214
BstMBI GATC 5 cut(s) 130, 192, 234, 1026, 1518
BstMWI GCNNNNNNNGC 5 cut(s) 787, 880, 947, 1078, 1626
BstNI CCWGG 2 cut(s) 15, 1421
BstNSI RCATGY 1 cut(s) 1748
BstSCI CCNGG 3 cut(s) 13, 340, 1419
BstSLI GKGCMC 1 cut(s) 663
BstV1I GCAGC 6 cut(s) 339, 870, 1002, 1069, 1282, 1498
BstX2I RGATCY 4 cut(s) 130, 192, 1026, 1518
BstYI RGATCY 4 cut(s) 130, 192, 1026, 1518
BsuRI GGCC 8 cut(s) 34, 231, 249, 950, 994, 1035, 1149, 1248
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 6 cut(s) 177, 292, 555, 883, 1297, 1664
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 5 cut(s) 661, 851, 1001, 1477, 1636
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 3 cut(s) 332, 1746, 1812
CfoI GCGC 2 cut(s) 1620, 1728
Cfr10I RCCGGY 1 cut(s) 404
Cfr13I GGNCC 3 cut(s) 119, 247, 407
CpoI CGGWCCG 1 cut(s) 407
Csp6I GTAC 6 cut(s) 61, 718, 727, 976, 1114, 1641
CspI CGGWCCG 1 cut(s) 407
CviQI GTAC 6 cut(s) 61, 718, 727, 976, 1114, 1641
DdeI CTNAG 5 cut(s) 954, 1303, 1358, 1479, 1860
DpnI GATC 5 cut(s) 132, 194, 236, 1028, 1520
DpnII GATC 5 cut(s) 130, 192, 234, 1026, 1518
DraIII CACNNNGTG 1 cut(s) 1005
DrdI GACNNNNNNGTC 1 cut(s) 380
DseDI GACNNNNNNGTC 1 cut(s) 380
EaeI YGGCCR 2 cut(s) 32, 1033
Eam1104I CTCTTC 5 cut(s) 450, 566, 858, 1702, 1824
EarI CTCTTC 5 cut(s) 450, 566, 858, 1702, 1824
Ecl136II GAGCTC 2 cut(s) 443, 676
Eco130I CCWWGG 5 cut(s) 35, 77, 322, 945, 1749
Eco24I GRGCYC 2 cut(s) 445, 678
Eco31I GGTCTC 1 cut(s) 1214
Eco32I GATATC 1 cut(s) 209
Eco47I GGWCC 2 cut(s) 119, 407
Eco53kI GAGCTC 2 cut(s) 443, 676
EcoICRI GAGCTC 2 cut(s) 443, 676
EcoNI CCTNNNNNAGG 2 cut(s) 1154, 1373
EcoO109I RGGNCCY 2 cut(s) 119, 247
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 13, 1419
EcoRV GATATC 1 cut(s) 209
EcoT14I CCWWGG 5 cut(s) 35, 77, 322, 945, 1749
EcoT38I GRGCYC 2 cut(s) 445, 678
ErhI CCWWGG 5 cut(s) 35, 77, 322, 945, 1749
FalI AAGNNNNNCTT 4 cut(s) 1341, 1373, 1803, 1835
FaqI GGGAC 2 cut(s) 610, 1354
FblI GTMKAC 3 cut(s) 240, 267, 827
Fnu4HI GCNGC 8 cut(s) 328, 884, 1016, 1036, 1058, 1246, 1271, 1512
FokI GGATG 6 cut(s) 184, 299, 542, 890, 1304, 1651
FriOI GRGCYC 2 cut(s) 445, 678
Fsp4HI GCNGC 8 cut(s) 328, 884, 1016, 1036, 1058, 1246, 1271, 1512
FspBI CTAG 1 cut(s) 323
FspI TGCGCA 1 cut(s) 1619
GlaI GCGC 2 cut(s) 1619, 1727
GluI GCNGC 8 cut(s) 328, 884, 1016, 1036, 1058, 1246, 1271, 1512
GsaI CCCAGC 1 cut(s) 1649
HaeIII GGCC 8 cut(s) 34, 231, 249, 950, 994, 1035, 1149, 1248
HapII CCGG 7 cut(s) 232, 342, 405, 410, 875, 929, 1734
HhaI GCGC 2 cut(s) 1620, 1728
Hin6I GCGC 2 cut(s) 1618, 1726
HinP1I GCGC 2 cut(s) 1618, 1726
HincII GTYRAC 4 cut(s) 112, 241, 268, 591
HindII GTYRAC 4 cut(s) 112, 241, 268, 591
HindIII AAGCTT 2 cut(s) 52, 1070
HinfI GANTC 9 cut(s) 40, 523, 833, 845, 869, 911, 1216, 1264, 1416
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 7 cut(s) 232, 342, 405, 410, 875, 929, 1734
HphI GGTGA 5 cut(s) 440, 493, 518, 589, 1450
Hpy166II GTNNAC 8 cut(s) 88, 112, 241, 268, 591, 729, 828, 1258
Hpy188I TCNGA 8 cut(s) 50, 767, 891, 1221, 1335, 1444, 1792, 1863
Hpy188III TCNNGA 3 cut(s) 204, 1051, 1213
Hpy8I GTNNAC 8 cut(s) 88, 112, 241, 268, 591, 729, 828, 1258
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 5 cut(s) 110, 361, 1405, 1600, 1748
HpyCH4III ACNGT 5 cut(s) 1005, 1106, 1557, 1640, 1852
HpyCH4IV ACGT 2 cut(s) 63, 389
HpyCH4V TGCA 8 cut(s) 666, 886, 1081, 1111, 1499, 1514, 1565, 1744
HpyF10VI GCNNNNNNNGC 5 cut(s) 787, 880, 947, 1078, 1626
HpyF3I CTNAG 5 cut(s) 954, 1303, 1358, 1479, 1860
HpySE526I ACGT 2 cut(s) 63, 389
HspAI GCGC 2 cut(s) 1618, 1726
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 5 cut(s) 130, 192, 234, 1026, 1518
LguI GCTCTTC 1 cut(s) 450
LmnI GCTCC 4 cut(s) 79, 180, 1160, 1228
Lsp1109I GCAGC 6 cut(s) 339, 870, 1002, 1069, 1282, 1498
LweI GCATC 3 cut(s) 162, 620, 895
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 63, 389
MaeIII GTNAC 5 cut(s) 499, 577, 997, 1778, 1852
MalI GATC 5 cut(s) 132, 194, 236, 1028, 1520
MbiI CCGCTC 1 cut(s) 157
MboI GATC 5 cut(s) 130, 192, 234, 1026, 1518
MboII GAAGA 9 cut(s) 437, 507, 553, 840, 875, 1346, 1562, 1719, 1811
MflI RGATCY 4 cut(s) 130, 192, 1026, 1518
MhlI GDGCHC 3 cut(s) 445, 663, 678
MlsI TGGCCA 1 cut(s) 34
MluCI AATT 7 cut(s) 254, 567, 667, 761, 772, 1076, 1613
MluNI TGGCCA 1 cut(s) 34
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 2 cut(s) 1739, 1770
Mox20I TGGCCA 1 cut(s) 34
MroXI GAANNNNTTC 1 cut(s) 1353
MscI TGGCCA 1 cut(s) 34
MseI TTAA 7 cut(s) 111, 714, 899, 1074, 1199, 1323, 1797
MslI CAYNNNNRTG 1 cut(s) 1043
Msp20I TGGCCA 1 cut(s) 34
MspA1I CMGCKG 2 cut(s) 1060, 1273
MspCI CTTAAG 1 cut(s) 713
MspI CCGG 7 cut(s) 232, 342, 405, 410, 875, 929, 1734
MspR9I CCNGG 3 cut(s) 15, 342, 1421
Mva1269I GAATGC 2 cut(s) 1329, 1567
MvaI CCWGG 2 cut(s) 15, 1421
MwoI GCNNNNNNNGC 5 cut(s) 787, 880, 947, 1078, 1626
NciI CCSGG 1 cut(s) 342
NcoI CCATGG 1 cut(s) 35
NdeII GATC 5 cut(s) 130, 192, 234, 1026, 1518
NlaIV GGNNCC 2 cut(s) 75, 335
NmeAIII GCCGAG 1 cut(s) 1201
NmuCI GTSAC 4 cut(s) 499, 577, 997, 1852
NsbI TGCGCA 1 cut(s) 1619
NspI RCATGY 1 cut(s) 1748
OliI CACNNNNGTG 1 cut(s) 1043
PaeI GCATGC 1 cut(s) 1748
PaqCI CACCTGC 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 2 cut(s) 1329, 1567
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 8 cut(s) 523, 833, 845, 869, 911, 1216, 1264, 1416
PkrI GCNGC 8 cut(s) 329, 885, 1017, 1037, 1059, 1247, 1272, 1513
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 64
PpuMI RGGWCCY 1 cut(s) 119
Psp124BI GAGCTC 2 cut(s) 445, 678
Psp1406I AACGTT 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 119
Psp6I CCWGG 2 cut(s) 13, 1419
PspFI CCCAGC 1 cut(s) 1645
PspGI CCWGG 2 cut(s) 13, 1419
PspN4I GGNNCC 2 cut(s) 75, 335
PspPI GGNCC 3 cut(s) 119, 247, 407
PspPPI RGGWCCY 1 cut(s) 119
PsuI RGATCY 4 cut(s) 130, 192, 1026, 1518
PvuII CAGCTG 1 cut(s) 1273
RsaI GTAC 6 cut(s) 62, 719, 728, 977, 1115, 1642
RsaNI GTAC 6 cut(s) 61, 718, 727, 976, 1114, 1641
RseI CAYNNNNRTG 1 cut(s) 1043
Rsr2I CGGWCCG 1 cut(s) 407
RsrII CGGWCCG 1 cut(s) 407
SacI GAGCTC 2 cut(s) 445, 678
SalI GTCGAC 2 cut(s) 239, 266
SapI GCTCTTC 1 cut(s) 450
SaqAI TTAA 7 cut(s) 111, 714, 899, 1074, 1199, 1323, 1797
SatI GCNGC 8 cut(s) 328, 884, 1016, 1036, 1058, 1246, 1271, 1512
Sau3AI GATC 5 cut(s) 130, 192, 234, 1026, 1518
Sau96I GGNCC 3 cut(s) 119, 247, 407
ScaI AGTACT 2 cut(s) 719, 1115
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 3 cut(s) 15, 342, 1421
SduI GDGCHC 3 cut(s) 445, 663, 678
SfaNI GCATC 3 cut(s) 162, 620, 895
SinI GGWCC 2 cut(s) 119, 407
SmiMI CAYNNNNRTG 1 cut(s) 1043
SmlI CTYRAG 2 cut(s) 713, 1670
SmoI CTYRAG 2 cut(s) 713, 1670
SphI GCATGC 1 cut(s) 1748
Sse9I AATT 7 cut(s) 254, 567, 667, 761, 772, 1076, 1613
SsiI CCGC 9 cut(s) 155, 216, 345, 536, 1036, 1060, 1245, 1268, 1776
SspI AATATT 2 cut(s) 300, 312
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 2 cut(s) 445, 678
StyD4I CCNGG 3 cut(s) 13, 340, 1419
StyI CCWWGG 5 cut(s) 35, 77, 322, 945, 1749
TaaI ACNGT 5 cut(s) 1005, 1106, 1557, 1640, 1852
TaiI ACGT 2 cut(s) 66, 392
TasI AATT 7 cut(s) 254, 567, 667, 761, 772, 1076, 1613
TatI WGTACW 3 cut(s) 717, 726, 1113
TauI GCSGC 2 cut(s) 1038, 1248
TfiI GAWTC 8 cut(s) 523, 833, 845, 869, 911, 1216, 1264, 1416
Tru1I TTAA 7 cut(s) 111, 714, 899, 1074, 1199, 1323, 1797
Tru9I TTAA 7 cut(s) 111, 714, 899, 1074, 1199, 1323, 1797
TscAI CASTG 5 cut(s) 668, 858, 1008, 1477, 1643
TseFI GTSAC 4 cut(s) 499, 577, 997, 1852
TseI GCWGC 6 cut(s) 327, 883, 1015, 1057, 1270, 1511
Tsp45I GTSAC 4 cut(s) 499, 577, 997, 1852
TspDTI ATGAA 7 cut(s) 94, 544, 662, 712, 740, 858, 1667
TspGWI ACGGA 2 cut(s) 321, 833
TspRI CASTG 5 cut(s) 668, 858, 1008, 1477, 1643
Vha464I CTTAAG 1 cut(s) 713
VpaK11BI GGWCC 2 cut(s) 119, 407
XagI CCTNNNNNAGG 2 cut(s) 1154, 1373
XapI RAATTY 1 cut(s) 254
XceI RCATGY 1 cut(s) 1748
XmaJI CCTAGG 1 cut(s) 322
XmiI GTMKAC 3 cut(s) 240, 267, 827
XmnI GAANNNNTTC 1 cut(s) 1353
XspI CTAG 1 cut(s) 323
ZrmI AGTACT 2 cut(s) 719, 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.