RchiOBHm_Chr1g0343371

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000300
Physical Location & Seq
Forward (+)
35435928 .. 35440331
4404 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56990

Sequence Viewer

Length: 1845 bp
ATGGCTTCCAATCCTGGCATTCTGACTGAATGGCCATGGACACCTCTTGGAAACTTTAAGTACGTGGTGGCTGCTCCCTGGGCGATACACAGCATATTCTCGTTCATTGTTAATGAGGGAGAAGACAGAGATCTATCTTACTTCCTCATATTTCCATTCATGTTGTTCAGGATGCTGCACAACCAGTTATGGATCTCTCTTTCTCGATATAAAACATCCAAAGGCAGCGGCCGGATCATCGACAAGGGTCTAGAGTTCGAACAAGTTGACAGAGAAAGAAACTGGGATGACCAAATTATATTCAATGGACTATTGTTCTTCCTAGGCAGCAGGCACTTGACCGGAGGTCGAAACCTACCGATGTGGAGAGGAGATGGATTCGTTATAACATTTCTGCTTCATGCTGGTCCAGTGGAGTTTCTTTACTACTGGTTGCATAGAGCTCTGCACCACCATTACCTATACTCTCGCTACCATTCTCACCACCATTCCTCCATTGTCACTGAGCCAATTACTTCTGTAATTCACCCATTTGGGGAGCACATAGCATATTTCCTCCTTTTCGCAATACCCTTGTACACAATGGCGTTGACCGGAACAGGTTCAATCATAAGCTATGCAGTTTATGTTACTTACATTGACTTAATGAACAACATGGGACACTGCAACTTCGAGCTCATTCCAAATTGGACCTTCTCTCTTTTTCCTCCTCTCAAGTATTTCATATATACCCCATCGTACCACTCTTTGCATCACACCCTATTTCGAACCAATTATTGCCTCTTCATGCCACTTTATGATTACATTTATGGCACCATGGAAAAGACTAGTGATGAACTCTATGAATCTTCACTCAAAAGAAAAGATGAAGCTCCAGATGTGTTGCATCTAACCCATCTTACAACACCCGACTCCATCTATCAACTTCCTTTGGGATTTGCTTCCTTGGCCTCTGAGCCGCACACTTCAACATGGTACATGTGGTTGATGTGGCCTGTTACATTTTGGTCGATGATACTCACATGGATTTATGGTCGCACATTTGTGGTTGAGAGGCAGCGGTTTGATAAACTCAAATTACAAACTTGGGCGATCCCAAAATACAATTTGCAATACTTCTTGCCATGGCAAAATGAAGCTATCAACAGTTTGATTGAGGAAGCTATACTTAAAGCAGAGGAAAAGGGTGTCAAAGTTGGTGGGGAGCTGAATAGATATGGTGGTCTATATACTCGGAGATATCCTCAGCTGAAAGTAAAGGTAGTGGATGGAAGTAGCTTAGCTGTGGCTGTAATCCTCAACAGCATACCAAAAGAGACAACCCAAGTTGTTCTTACAGGCAACCTTACAAAGGTCGCTTATGCAATTGCATTTGCTTTGGGCCAGAGGGGCATTGAGGTAGCTACATTATACAAGGATGAGTATTTGAAGCTCACCAAATCAGTAAGTGCTACTAAGAGTGAGGTGGTTCTTGCAAAAGGCTCTGGTTCAAAGATTTGGTTAGTGGGAGATGGATTGAGAAAAGATCAACTAAATGCACCAAAAGGAACAATGTTTGTTCCCTTCTCCCAATTCCCCCTTAAAAAGTTGCGCAGGGACTGCTTCTACCACTGCACTCCAGCAATGAAGATCCCCCGATCTCTTGAGAATGTCTACTCGTGTGAGAACTGGTTGCCAAGAAGGGTGATGAGCGCATGGCGCATAGCTGGAATAGTGCATGCCTTGGAAGGTTGGAATGAACATGAGTGTGGTTCCGCCATGTCCGACATCAACAAGGTTTGGGAAGCTACGCTTCGACATGGCTTTCAGCCTCTAATCCCCAATGCTACTCCGAACAAATATTAA

Protein Analysis

614

Amino Acids

71.02

Weight (kDa)

8.78

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 133 - 272 3.8e-20 Fatty acid hydroxylase
CER1-like_C PF12076 443 - 604 4.7e-70 CER1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 386
Acc16I TGCGCA 1 cut(s) 1592
AccB1I GGYRCC 1 cut(s) 812
AccI GTMKAC 1 cut(s) 1653
AciI CCGC 4 cut(s) 228, 959, 1060, 1755
AclWI GGATC 4 cut(s) 200, 242, 1087, 1624
AcoI YGGCCR 2 cut(s) 32, 229
AfaI GTAC 4 cut(s) 62, 578, 740, 977
AfiI CCNNNNNNNGG 2 cut(s) 535, 1351
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 5 cut(s) 304, 606, 969, 1429, 1491
AhdI GACNNNNNGTC 1 cut(s) 345
AhlI ACTAGT 1 cut(s) 827
AjnI CCWGG 2 cut(s) 13, 77
AleI CACNNNNGTG 1 cut(s) 1043
Alw21I GWGCWC 3 cut(s) 445, 543, 678
Alw26I GTCTC 1 cut(s) 1310
AlwI GGATC 4 cut(s) 200, 242, 1087, 1624
AlwNI CAGNNNCTG 1 cut(s) 1599
AoxI GGCC 5 cut(s) 32, 229, 948, 992, 1381
ApeKI GCWGC 5 cut(s) 71, 175, 225, 327, 1057
Asp700I GAANNNNTTC 1 cut(s) 601
AspA2I CCTAGG 1 cut(s) 322
AspLEI GCGC 3 cut(s) 1593, 1694, 1701
AspS9I GGNCC 3 cut(s) 407, 690, 1381
AsuHPI GGTGA 4 cut(s) 473, 518, 1426, 1696
AsuII TTCGAA 2 cut(s) 258, 766
AvaII GGWCC 2 cut(s) 407, 690
AvrII CCTAGG 1 cut(s) 322
BalI TGGCCA 1 cut(s) 34
BanI GGYRCC 1 cut(s) 812
BanII GRGCYC 2 cut(s) 445, 678
BauI CACGAG 1 cut(s) 1657
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 3 cut(s) 445, 543, 678
BbvCI CCTCAGC 1 cut(s) 1245
BbvI GCAGC 5 cut(s) 58, 162, 237, 339, 1069
BccI CCATC 6 cut(s) 368, 742, 903, 923, 1262, 1505
BciT130I CCWGG 2 cut(s) 15, 79
BcoDI GTCTC 1 cut(s) 1310
BcuI ACTAGT 1 cut(s) 827
BfaI CTAG 3 cut(s) 251, 323, 828
BglI GCCNNNNNGGC 1 cut(s) 1389
BglII AGATCT 1 cut(s) 130
BisI GCNGC 7 cut(s) 72, 176, 226, 229, 328, 959, 1058
BlnI CCTAGG 1 cut(s) 322
BlpI GCTNAGC 1 cut(s) 1279
BlsI GCNGC 7 cut(s) 73, 177, 227, 230, 329, 960, 1059
Bme1390I CCNGG 2 cut(s) 15, 79
Bme18I GGWCC 2 cut(s) 407, 690
BmeRI GACNNNNNGTC 1 cut(s) 345
BmgT120I GGNCC 3 cut(s) 407, 690, 1381
BmiI GGNNCC 2 cut(s) 814, 1753
BmrFI CCNGG 2 cut(s) 15, 79
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 3 cut(s) 162, 760, 895
BmuI ACTGGG 1 cut(s) 292
BoxI GACNNNNGTC 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 129
BplI GAGNNNNNCTC 2 cut(s) 1228, 1260
BpmI CTGGAG 2 cut(s) 858, 1602
Bpu10I CCTNAGC 1 cut(s) 1245
Bpu1102I GCTNAGC 1 cut(s) 1279
Bpu14I TTCGAA 2 cut(s) 258, 766
BpuEI CTTGAG 2 cut(s) 698, 1664
BsaAI YACGTR 1 cut(s) 64
BsaJI CCNNGG 8 cut(s) 35, 77, 78, 322, 816, 945, 1124, 1722
BsaWI WCCGGW 2 cut(s) 341, 593
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 535, 1351
Bse1I ACTGG 5 cut(s) 184, 287, 410, 434, 1673
Bse3DI GCAATG 1 cut(s) 1629
BseBI CCWGG 2 cut(s) 15, 79
BseDI CCNNGG 8 cut(s) 35, 77, 78, 322, 816, 945, 1124, 1722
BseGI GGATG 5 cut(s) 177, 215, 292, 1273, 1423
BseLI CCNNNNNNNGG 2 cut(s) 535, 1351
BseMI GCAATG 1 cut(s) 1629
BseMII CTCAG 3 cut(s) 495, 945, 1259
BseNI ACTGG 5 cut(s) 184, 287, 410, 434, 1673
BseRI GAGGAG 2 cut(s) 384, 699
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 5 cut(s) 58, 162, 237, 339, 1069
BsgI GTGCAG 3 cut(s) 161, 431, 1597
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 5 cut(s) 34, 231, 950, 994, 1383
BshNI GGYRCC 1 cut(s) 812
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 3 cut(s) 445, 543, 678
BsiSI CCGG 3 cut(s) 232, 342, 594
BslFI GGGAC 2 cut(s) 672, 1610
BslI CCNNNNNNNGG 2 cut(s) 535, 1351
BsmAI GTCTC 1 cut(s) 1310
BsmFI GGGAC 2 cut(s) 672, 1610
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 5 cut(s) 34, 231, 950, 994, 1383
Bsp119I TTCGAA 2 cut(s) 258, 766
Bsp1286I GDGCHC 3 cut(s) 445, 543, 678
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
Bsp1720I GCTNAGC 1 cut(s) 1279
Bsp19I CCATGG 3 cut(s) 35, 816, 1124
BspACI CCGC 4 cut(s) 228, 959, 1060, 1755
BspANI GGCC 5 cut(s) 34, 231, 950, 994, 1383
BspCNI CTCAG 3 cut(s) 496, 946, 1258
BspLI GGNNCC 2 cut(s) 814, 1753
BspPI GGATC 4 cut(s) 200, 242, 1087, 1624
BspT104I TTCGAA 2 cut(s) 258, 766
BspT107I GGYRCC 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 1629
BsrGI TGTACA 1 cut(s) 576
BsrI ACTGG 5 cut(s) 184, 287, 410, 434, 1673
BssECI CCNNGG 8 cut(s) 35, 77, 78, 322, 816, 945, 1124, 1722
BssMI GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
BssSI CACGAG 1 cut(s) 1657
BssT1I CCWWGG 6 cut(s) 35, 322, 816, 945, 1124, 1722
Bst2BI CACGAG 1 cut(s) 1657
Bst2UI CCWGG 2 cut(s) 15, 79
Bst4CI ACNGT 1 cut(s) 1148
Bst6I CTCTTC 1 cut(s) 788
BstAPI GCANNNNNTGC 1 cut(s) 1599
BstAUI TGTACA 1 cut(s) 576
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 2 cut(s) 258, 766
BstC8I GCNNGC 2 cut(s) 332, 1719
BstDEI CTNAG 5 cut(s) 504, 954, 1245, 1279, 1455
BstDSI CCRYGG 3 cut(s) 35, 816, 1124
BstENI CCTNNNNNAGG 1 cut(s) 1349
BstF5I GGATG 5 cut(s) 177, 215, 292, 1273, 1423
BstHHI GCGC 3 cut(s) 1593, 1694, 1701
BstKTI GATC 7 cut(s) 133, 195, 237, 1095, 1528, 1632, 1640
BstMAI GTCTC 1 cut(s) 1310
BstMBI GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 5 cut(s) 80, 947, 1389, 1599, 1698
BstNI CCWGG 2 cut(s) 15, 79
BstNSI RCATGY 2 cut(s) 982, 1721
BstPAI GACNNNNGTC 1 cut(s) 246
BstSCI CCNGG 2 cut(s) 13, 77
BstV1I GCAGC 5 cut(s) 58, 162, 237, 339, 1069
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 3 cut(s) 130, 192, 1629
BstYI RGATCY 3 cut(s) 130, 192, 1629
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 5 cut(s) 34, 231, 950, 994, 1383
BtgI CCRYGG 3 cut(s) 35, 816, 1124
BtsCI GGATG 5 cut(s) 177, 215, 292, 1273, 1423
BtsI GCAGTG 2 cut(s) 661, 1609
BtsIMutI CAGTG 4 cut(s) 417, 501, 661, 1609
Cac8I GCNNGC 2 cut(s) 332, 1719
CaiI CAGNNNCTG 1 cut(s) 1599
CfoI GCGC 3 cut(s) 1593, 1694, 1701
Cfr13I GGNCC 3 cut(s) 407, 690, 1381
Csp6I GTAC 4 cut(s) 61, 577, 739, 976
CspCI CAANNNNNGTGG 2 cut(s) 1180, 1215
CviQI GTAC 4 cut(s) 61, 577, 739, 976
DdeI CTNAG 5 cut(s) 504, 954, 1245, 1279, 1455
DpnI GATC 7 cut(s) 132, 194, 236, 1094, 1527, 1631, 1639
DpnII GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
DriI GACNNNNNGTC 1 cut(s) 345
EaeI YGGCCR 2 cut(s) 32, 229
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 788
Eam1105I GACNNNNNGTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 788
EciI GGCGGA 1 cut(s) 1744
Ecl136II GAGCTC 2 cut(s) 443, 676
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 6 cut(s) 35, 322, 816, 945, 1124, 1722
Eco24I GRGCYC 2 cut(s) 445, 678
Eco32I GATATC 1 cut(s) 1241
Eco47I GGWCC 2 cut(s) 407, 690
Eco52I CGGCCG 1 cut(s) 229
Eco53kI GAGCTC 2 cut(s) 443, 676
EcoICRI GAGCTC 2 cut(s) 443, 676
EcoNI CCTNNNNNAGG 1 cut(s) 1349
EcoRII CCWGG 2 cut(s) 13, 77
EcoRV GATATC 1 cut(s) 1241
EcoT14I CCWWGG 6 cut(s) 35, 322, 816, 945, 1124, 1722
EcoT38I GRGCYC 2 cut(s) 445, 678
ErhI CCWWGG 6 cut(s) 35, 322, 816, 945, 1124, 1722
FalI AAGNNNNNCTT 6 cut(s) 1152, 1184, 1317, 1349, 1776, 1808
FaqI GGGAC 2 cut(s) 672, 1610
FblI GTMKAC 1 cut(s) 1653
Fnu4HI GCNGC 7 cut(s) 72, 176, 226, 229, 328, 959, 1058
FokI GGATG 5 cut(s) 184, 202, 299, 1280, 1430
FriOI GRGCYC 2 cut(s) 445, 678
Fsp4HI GCNGC 7 cut(s) 72, 176, 226, 229, 328, 959, 1058
FspBI CTAG 3 cut(s) 251, 323, 828
FspI TGCGCA 1 cut(s) 1592
GlaI GCGC 3 cut(s) 1592, 1693, 1700
GluI GCNGC 7 cut(s) 72, 176, 226, 229, 328, 959, 1058
GsuI CTGGAG 2 cut(s) 858, 1602
HaeIII GGCC 5 cut(s) 34, 231, 950, 994, 1383
HapII CCGG 3 cut(s) 232, 342, 594
HhaI GCGC 3 cut(s) 1593, 1694, 1701
Hin6I GCGC 3 cut(s) 1591, 1692, 1699
HinP1I GCGC 3 cut(s) 1591, 1692, 1699
HincII GTYRAC 2 cut(s) 268, 591
HindII GTYRAC 2 cut(s) 268, 591
HinfI GANTC 3 cut(s) 378, 845, 911
HpaII CCGG 3 cut(s) 232, 342, 594
HphI GGTGA 4 cut(s) 473, 518, 1426, 1696
Hpy166II GTNNAC 4 cut(s) 268, 579, 591, 1654
Hpy188I TCNGA 5 cut(s) 24, 955, 1236, 1765, 1833
Hpy188III TCNNGA 5 cut(s) 169, 204, 251, 875, 1643
Hpy8I GTNNAC 4 cut(s) 268, 579, 591, 1654
HpyAV CCTTC 4 cut(s) 703, 1573, 1674, 1721
HpyCH4III ACNGT 1 cut(s) 1148
HpyCH4IV ACGT 1 cut(s) 63
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 947, 1389, 1599, 1698
HpyF3I CTNAG 5 cut(s) 504, 954, 1245, 1279, 1455
HpySE526I ACGT 1 cut(s) 63
HspAI GCGC 3 cut(s) 1591, 1692, 1699
Kzo9I GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
LmnI GCTCC 4 cut(s) 79, 538, 877, 1204
Lsp1109I GCAGC 5 cut(s) 58, 162, 237, 339, 1069
LweI GCATC 3 cut(s) 162, 760, 895
MaeI CTAG 3 cut(s) 251, 323, 828
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 3 cut(s) 499, 628, 997
MalI GATC 7 cut(s) 132, 194, 236, 1094, 1527, 1631, 1639
MboI GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
MboII GAAGA 5 cut(s) 134, 310, 775, 840, 1639
MfeI CAATTG 1 cut(s) 1365
MflI RGATCY 3 cut(s) 130, 192, 1629
MhlI GDGCHC 3 cut(s) 445, 543, 678
MlsI TGGCCA 1 cut(s) 34
MluCI AATT 9 cut(s) 294, 510, 522, 685, 772, 1076, 1105, 1365, 1571
MluNI TGGCCA 1 cut(s) 34
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 2 cut(s) 1712, 1788
Mox20I TGGCCA 1 cut(s) 34
MroXI GAANNNNTTC 1 cut(s) 601
MscI TGGCCA 1 cut(s) 34
MseI TTAA 6 cut(s) 57, 111, 644, 1170, 1581, 1843
MslI CAYNNNNRTG 2 cut(s) 1043, 1746
Msp20I TGGCCA 1 cut(s) 34
MspA1I CMGCKG 3 cut(s) 228, 1060, 1249
MspI CCGG 3 cut(s) 232, 342, 594
MspR9I CCNGG 2 cut(s) 15, 79
MunI CAATTG 1 cut(s) 1365
Mva1269I GAATGC 1 cut(s) 18
MvaI CCWGG 2 cut(s) 15, 79
MwoI GCNNNNNNNGC 5 cut(s) 80, 947, 1389, 1599, 1698
NcoI CCATGG 3 cut(s) 35, 816, 1124
NdeII GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
NlaIV GGNNCC 2 cut(s) 814, 1753
NmuCI GTSAC 1 cut(s) 499
NsbI TGCGCA 1 cut(s) 1592
NspI RCATGY 2 cut(s) 982, 1721
NspV TTCGAA 2 cut(s) 258, 766
OliI CACNNNNGTG 1 cut(s) 1043
PaeI GCATGC 1 cut(s) 1721
PasI CCCWGGG 1 cut(s) 78
PciI ACATGT 1 cut(s) 978
PctI GAATGC 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 601
PfeI GAWTC 2 cut(s) 378, 845
PkrI GCNGC 7 cut(s) 73, 177, 227, 230, 329, 960, 1059
PleI GAGTC 1 cut(s) 905
PpsI GAGTC 1 cut(s) 905
Ppu21I YACGTR 1 cut(s) 64
PscI ACATGT 1 cut(s) 978
PshAI GACNNNNGTC 1 cut(s) 246
PsiI TTATAA 1 cut(s) 386
Psp124BI GAGCTC 2 cut(s) 445, 678
Psp6I CCWGG 2 cut(s) 13, 77
PspGI CCWGG 2 cut(s) 13, 77
PspN4I GGNNCC 2 cut(s) 814, 1753
PspPI GGNCC 3 cut(s) 407, 690, 1381
PstNI CAGNNNCTG 1 cut(s) 1599
PsuI RGATCY 3 cut(s) 130, 192, 1629
PvuII CAGCTG 1 cut(s) 1249
RsaI GTAC 4 cut(s) 62, 578, 740, 977
RsaNI GTAC 4 cut(s) 61, 577, 739, 976
RseI CAYNNNNRTG 2 cut(s) 1043, 1746
SacI GAGCTC 2 cut(s) 445, 678
SaqAI TTAA 6 cut(s) 57, 111, 644, 1170, 1581, 1843
SatI GCNGC 7 cut(s) 72, 176, 226, 229, 328, 959, 1058
Sau3AI GATC 7 cut(s) 130, 192, 234, 1092, 1525, 1629, 1637
Sau96I GGNCC 3 cut(s) 407, 690, 1381
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 15, 79
SduI GDGCHC 3 cut(s) 445, 543, 678
SfaNI GCATC 3 cut(s) 162, 760, 895
SfuI TTCGAA 2 cut(s) 258, 766
SinI GGWCC 2 cut(s) 407, 690
SmiMI CAYNNNNRTG 2 cut(s) 1043, 1746
SmlI CTYRAG 2 cut(s) 713, 1643
SmoI CTYRAG 2 cut(s) 713, 1643
SpeI ACTAGT 1 cut(s) 827
SphI GCATGC 1 cut(s) 1721
Sse9I AATT 9 cut(s) 294, 510, 522, 685, 772, 1076, 1105, 1365, 1571
SsiI CCGC 4 cut(s) 228, 959, 1060, 1755
SspI AATATT 1 cut(s) 1841
SspMI CTAG 3 cut(s) 251, 323, 828
SstI GAGCTC 2 cut(s) 445, 678
StyD4I CCNGG 2 cut(s) 13, 77
StyI CCWWGG 6 cut(s) 35, 322, 816, 945, 1124, 1722
TaaI ACNGT 1 cut(s) 1148
TaiI ACGT 1 cut(s) 66
TaqI TCGA 8 cut(s) 205, 240, 258, 349, 672, 766, 1010, 1795
TasI AATT 9 cut(s) 294, 510, 522, 685, 772, 1076, 1105, 1365, 1571
TatI WGTACW 1 cut(s) 576
TauI GCSGC 2 cut(s) 231, 961
TfiI GAWTC 2 cut(s) 378, 845
Tru1I TTAA 6 cut(s) 57, 111, 644, 1170, 1581, 1843
Tru9I TTAA 6 cut(s) 57, 111, 644, 1170, 1581, 1843
TscAI CASTG 4 cut(s) 417, 508, 668, 1616
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 5 cut(s) 71, 175, 225, 327, 1057
Tsp45I GTSAC 1 cut(s) 499
TspRI CASTG 4 cut(s) 417, 508, 668, 1616
VpaK11BI GGWCC 2 cut(s) 407, 690
XagI CCTNNNNNAGG 1 cut(s) 1349
XbaI TCTAGA 1 cut(s) 250
XceI RCATGY 2 cut(s) 982, 1721
XmaJI CCTAGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 1653
XmnI GAANNNNTTC 1 cut(s) 601
XspI CTAG 3 cut(s) 251, 323, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.