Belongs to the sterol desaturase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 386 |
| Acc16I | TGCGCA | 1 cut(s) | 1619 |
| AccB1I | GGYRCC | 1 cut(s) | 812 |
| AciI | CCGC | 3 cut(s) | 228, 959, 1060 |
| AclWI | GGATC | 4 cut(s) | 200, 242, 1087, 1651 |
| AcoI | YGGCCR | 2 cut(s) | 32, 229 |
| AcvI | CACGTG | 1 cut(s) | 337 |
| AfaI | GTAC | 4 cut(s) | 62, 578, 740, 977 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 535, 1225, 1375 |
| AflIII | ACRYGT | 1 cut(s) | 978 |
| AgsI | TTSAA | 5 cut(s) | 304, 606, 969, 1453, 1515 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 345 |
| AhlI | ACTAGT | 1 cut(s) | 827 |
| AjnI | CCWGG | 3 cut(s) | 13, 77, 1218 |
| AleI | CACNNNNGTG | 1 cut(s) | 1043 |
| Alw21I | GWGCWC | 3 cut(s) | 445, 543, 678 |
| Alw26I | GTCTC | 2 cut(s) | 1334, 1671 |
| AlwI | GGATC | 4 cut(s) | 200, 242, 1087, 1651 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1626, 1840 |
| AoxI | GGCC | 4 cut(s) | 32, 229, 948, 992 |
| ApeKI | GCWGC | 4 cut(s) | 175, 225, 327, 1057 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 601 |
| AspA2I | CCTAGG | 1 cut(s) | 322 |
| AspLEI | GCGC | 3 cut(s) | 1620, 1721, 1728 |
| AspS9I | GGNCC | 2 cut(s) | 407, 690 |
| AsuC2I | CCSGG | 1 cut(s) | 1663 |
| AsuHPI | GGTGA | 5 cut(s) | 473, 518, 1235, 1450, 1723 |
| AsuII | TTCGAA | 2 cut(s) | 258, 766 |
| AvaII | GGWCC | 2 cut(s) | 407, 690 |
| AvrII | CCTAGG | 1 cut(s) | 322 |
| BalI | TGGCCA | 1 cut(s) | 34 |
| BanI | GGYRCC | 1 cut(s) | 812 |
| BanII | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| BauI | CACGAG | 1 cut(s) | 1684 |
| BbrPI | CACGTG | 1 cut(s) | 337 |
| BbsI | GAAGAC | 2 cut(s) | 129, 1557 |
| Bbv12I | GWGCWC | 3 cut(s) | 445, 543, 678 |
| BbvCI | CCTCAGC | 1 cut(s) | 1269 |
| BbvI | GCAGC | 4 cut(s) | 162, 237, 339, 1069 |
| BccI | CCATC | 5 cut(s) | 368, 742, 903, 923, 1286 |
| BciT130I | CCWGG | 3 cut(s) | 15, 79, 1220 |
| BcnI | CCSGG | 1 cut(s) | 1663 |
| BcoDI | GTCTC | 2 cut(s) | 1334, 1671 |
| BcuI | ACTAGT | 1 cut(s) | 827 |
| BfaI | CTAG | 3 cut(s) | 251, 323, 828 |
| BglII | AGATCT | 1 cut(s) | 130 |
| BisI | GCNGC | 6 cut(s) | 176, 226, 229, 328, 959, 1058 |
| BlnI | CCTAGG | 1 cut(s) | 322 |
| BlpI | GCTNAGC | 1 cut(s) | 1303 |
| BlsI | GCNGC | 6 cut(s) | 177, 227, 230, 329, 960, 1059 |
| Bme1390I | CCNGG | 4 cut(s) | 15, 79, 1220, 1663 |
| Bme18I | GGWCC | 2 cut(s) | 407, 690 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 345 |
| BmgT120I | GGNCC | 2 cut(s) | 407, 690 |
| BmiI | GGNNCC | 1 cut(s) | 814 |
| BmrFI | CCNGG | 4 cut(s) | 15, 79, 1220, 1663 |
| BmrI | ACTGGG | 1 cut(s) | 292 |
| BmsI | GCATC | 4 cut(s) | 162, 760, 895, 1005 |
| BmuI | ACTGGG | 1 cut(s) | 292 |
| BoxI | GACNNNNGTC | 1 cut(s) | 246 |
| BpiI | GAAGAC | 2 cut(s) | 129, 1557 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1252, 1284 |
| BpmI | CTGGAG | 2 cut(s) | 858, 1629 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1269 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1303 |
| Bpu14I | TTCGAA | 2 cut(s) | 258, 766 |
| BpuEI | CTTGAG | 2 cut(s) | 698, 1691 |
| BpuMI | CCSGG | 1 cut(s) | 1663 |
| BsaAI | YACGTR | 2 cut(s) | 64, 337 |
| BsaI | GGTCTC | 1 cut(s) | 1671 |
| BsaWI | WCCGGW | 1 cut(s) | 341 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 476, 506 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 535, 1225, 1375 |
| Bse1I | ACTGG | 5 cut(s) | 184, 287, 410, 434, 1700 |
| Bse3DI | GCAATG | 1 cut(s) | 1656 |
| BseBI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| BseGI | GGATG | 5 cut(s) | 177, 215, 292, 1297, 1447 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 535, 1225, 1375 |
| BseMI | GCAATG | 1 cut(s) | 1656 |
| BseMII | CTCAG | 3 cut(s) | 495, 945, 1283 |
| BseNI | ACTGG | 5 cut(s) | 184, 287, 410, 434, 1700 |
| BseRI | GAGGAG | 2 cut(s) | 384, 1241 |
| BseX3I | CGGCCG | 1 cut(s) | 229 |
| BseXI | GCAGC | 4 cut(s) | 162, 237, 339, 1069 |
| BsgI | GTGCAG | 3 cut(s) | 161, 431, 1624 |
| Bsh1285I | CGRYCG | 1 cut(s) | 232 |
| BshFI | GGCC | 4 cut(s) | 34, 231, 950, 994 |
| BshNI | GGYRCC | 1 cut(s) | 812 |
| BsiEI | CGRYCG | 1 cut(s) | 232 |
| BsiHKAI | GWGCWC | 3 cut(s) | 445, 543, 678 |
| BsiSI | CCGG | 3 cut(s) | 232, 342, 1663 |
| BslFI | GGGAC | 2 cut(s) | 672, 1637 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 535, 1225, 1375 |
| BsmAI | GTCTC | 2 cut(s) | 1334, 1671 |
| BsmFI | GGGAC | 2 cut(s) | 672, 1637 |
| BsmI | GAATGC | 1 cut(s) | 18 |
| BsnI | GGCC | 4 cut(s) | 34, 231, 950, 994 |
| Bso31I | GGTCTC | 1 cut(s) | 1671 |
| Bsp119I | TTCGAA | 2 cut(s) | 258, 766 |
| Bsp1286I | GDGCHC | 4 cut(s) | 445, 543, 678, 1212 |
| Bsp1407I | TGTACA | 1 cut(s) | 576 |
| Bsp143I | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1303 |
| Bsp19I | CCATGG | 3 cut(s) | 35, 816, 1124 |
| BspACI | CCGC | 3 cut(s) | 228, 959, 1060 |
| BspANI | GGCC | 4 cut(s) | 34, 231, 950, 994 |
| BspCNI | CTCAG | 3 cut(s) | 496, 946, 1282 |
| BspLI | GGNNCC | 1 cut(s) | 814 |
| BspPI | GGATC | 4 cut(s) | 200, 242, 1087, 1651 |
| BspT104I | TTCGAA | 2 cut(s) | 258, 766 |
| BspT107I | GGYRCC | 1 cut(s) | 812 |
| BspTNI | GGTCTC | 1 cut(s) | 1671 |
| BsrDI | GCAATG | 1 cut(s) | 1656 |
| BsrGI | TGTACA | 1 cut(s) | 576 |
| BsrI | ACTGG | 5 cut(s) | 184, 287, 410, 434, 1700 |
| BssMI | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| BssSI | CACGAG | 1 cut(s) | 1684 |
| BssT1I | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| Bst2BI | CACGAG | 1 cut(s) | 1684 |
| Bst2UI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| Bst4CI | ACNGT | 1 cut(s) | 840 |
| Bst6I | CTCTTC | 1 cut(s) | 788 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1626 |
| BstAUI | TGTACA | 1 cut(s) | 576 |
| BstBAI | YACGTR | 2 cut(s) | 64, 337 |
| BstBI | TTCGAA | 2 cut(s) | 258, 766 |
| BstC8I | GCNNGC | 2 cut(s) | 332, 1746 |
| BstDEI | CTNAG | 4 cut(s) | 504, 954, 1269, 1303 |
| BstDSI | CCRYGG | 3 cut(s) | 35, 816, 1124 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| BstF5I | GGATG | 5 cut(s) | 177, 215, 292, 1297, 1447 |
| BstHHI | GCGC | 3 cut(s) | 1620, 1721, 1728 |
| BstKTI | GATC | 5 cut(s) | 133, 195, 237, 1095, 1659 |
| BstMAI | GTCTC | 2 cut(s) | 1334, 1671 |
| BstMBI | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| BstMCI | CGRYCG | 1 cut(s) | 232 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 80, 947, 1626, 1725 |
| BstNI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| BstNSI | RCATGY | 2 cut(s) | 982, 1748 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 246 |
| BstSCI | CCNGG | 4 cut(s) | 13, 77, 1218, 1661 |
| BstV1I | GCAGC | 4 cut(s) | 162, 237, 339, 1069 |
| BstV2I | GAAGAC | 2 cut(s) | 129, 1557 |
| BstX2I | RGATCY | 3 cut(s) | 130, 192, 1656 |
| BstYI | RGATCY | 3 cut(s) | 130, 192, 1656 |
| BstZI | CGGCCG | 1 cut(s) | 229 |
| BsuRI | GGCC | 4 cut(s) | 34, 231, 950, 994 |
| BtgI | CCRYGG | 3 cut(s) | 35, 816, 1124 |
| BtsCI | GGATG | 5 cut(s) | 177, 215, 292, 1297, 1447 |
| BtsI | GCAGTG | 2 cut(s) | 661, 1636 |
| BtsIMutI | CAGTG | 4 cut(s) | 417, 501, 661, 1636 |
| Cac8I | GCNNGC | 2 cut(s) | 332, 1746 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1626, 1840 |
| CfoI | GCGC | 3 cut(s) | 1620, 1721, 1728 |
| Cfr13I | GGNCC | 2 cut(s) | 407, 690 |
| Csp6I | GTAC | 4 cut(s) | 61, 577, 739, 976 |
| CviQI | GTAC | 4 cut(s) | 61, 577, 739, 976 |
| DdeI | CTNAG | 4 cut(s) | 504, 954, 1269, 1303 |
| DpnI | GATC | 5 cut(s) | 132, 194, 236, 1094, 1658 |
| DpnII | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| DriI | GACNNNNNGTC | 1 cut(s) | 345 |
| EaeI | YGGCCR | 2 cut(s) | 32, 229 |
| EagI | CGGCCG | 1 cut(s) | 229 |
| Eam1104I | CTCTTC | 1 cut(s) | 788 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 345 |
| EarI | CTCTTC | 1 cut(s) | 788 |
| Ecl136II | GAGCTC | 2 cut(s) | 443, 676 |
| EclXI | CGGCCG | 1 cut(s) | 229 |
| Eco130I | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| Eco24I | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| Eco31I | GGTCTC | 1 cut(s) | 1671 |
| Eco32I | GATATC | 1 cut(s) | 1265 |
| Eco47I | GGWCC | 2 cut(s) | 407, 690 |
| Eco52I | CGGCCG | 1 cut(s) | 229 |
| Eco53kI | GAGCTC | 2 cut(s) | 443, 676 |
| Eco72I | CACGTG | 1 cut(s) | 337 |
| EcoICRI | GAGCTC | 2 cut(s) | 443, 676 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| EcoRII | CCWGG | 3 cut(s) | 13, 77, 1218 |
| EcoRV | GATATC | 1 cut(s) | 1265 |
| EcoT14I | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| EcoT38I | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| ErhI | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1152, 1184, 1341, 1373, 1803, 1835 |
| FaqI | GGGAC | 2 cut(s) | 672, 1637 |
| Fnu4HI | GCNGC | 6 cut(s) | 176, 226, 229, 328, 959, 1058 |
| FokI | GGATG | 5 cut(s) | 184, 202, 299, 1304, 1454 |
| FriOI | GRGCYC | 3 cut(s) | 445, 678, 1212 |
| Fsp4HI | GCNGC | 6 cut(s) | 176, 226, 229, 328, 959, 1058 |
| FspBI | CTAG | 3 cut(s) | 251, 323, 828 |
| FspI | TGCGCA | 1 cut(s) | 1619 |
| GlaI | GCGC | 3 cut(s) | 1619, 1720, 1727 |
| GluI | GCNGC | 6 cut(s) | 176, 226, 229, 328, 959, 1058 |
| GsuI | CTGGAG | 2 cut(s) | 858, 1629 |
| HaeIII | GGCC | 4 cut(s) | 34, 231, 950, 994 |
| HapII | CCGG | 3 cut(s) | 232, 342, 1663 |
| HhaI | GCGC | 3 cut(s) | 1620, 1721, 1728 |
| Hin6I | GCGC | 3 cut(s) | 1618, 1719, 1726 |
| HinP1I | GCGC | 3 cut(s) | 1618, 1719, 1726 |
| HincII | GTYRAC | 1 cut(s) | 591 |
| HindII | GTYRAC | 1 cut(s) | 591 |
| HinfI | GANTC | 4 cut(s) | 378, 845, 911, 1843 |
| HpaII | CCGG | 3 cut(s) | 232, 342, 1663 |
| HphI | GGTGA | 5 cut(s) | 473, 518, 1235, 1450, 1723 |
| Hpy166II | GTNNAC | 4 cut(s) | 268, 579, 591, 1681 |
| Hpy188I | TCNGA | 5 cut(s) | 24, 955, 1792, 1842, 1860 |
| Hpy188III | TCNNGA | 4 cut(s) | 204, 251, 875, 1670 |
| Hpy8I | GTNNAC | 4 cut(s) | 268, 579, 591, 1681 |
| HpyAV | CCTTC | 5 cut(s) | 703, 1529, 1600, 1701, 1748 |
| HpyCH4III | ACNGT | 1 cut(s) | 840 |
| HpyCH4IV | ACGT | 2 cut(s) | 63, 336 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 80, 947, 1626, 1725 |
| HpyF3I | CTNAG | 4 cut(s) | 504, 954, 1269, 1303 |
| HpySE526I | ACGT | 2 cut(s) | 63, 336 |
| HspAI | GCGC | 3 cut(s) | 1618, 1719, 1726 |
| Kzo9I | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| LmnI | GCTCC | 4 cut(s) | 79, 538, 877, 1228 |
| Lsp1109I | GCAGC | 4 cut(s) | 162, 237, 339, 1069 |
| LweI | GCATC | 4 cut(s) | 162, 760, 895, 1005 |
| MaeI | CTAG | 3 cut(s) | 251, 323, 828 |
| MaeII | ACGT | 2 cut(s) | 63, 336 |
| MaeIII | GTNAC | 5 cut(s) | 337, 499, 628, 997, 1778 |
| MalI | GATC | 5 cut(s) | 132, 194, 236, 1094, 1658 |
| MboI | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| MboII | GAAGA | 6 cut(s) | 134, 310, 775, 840, 1562, 1666 |
| MfeI | CAATTG | 1 cut(s) | 1389 |
| MflI | RGATCY | 3 cut(s) | 130, 192, 1656 |
| MhlI | GDGCHC | 4 cut(s) | 445, 543, 678, 1212 |
| MlsI | TGGCCA | 1 cut(s) | 34 |
| MluNI | TGGCCA | 1 cut(s) | 34 |
| MlyI | GAGTC | 1 cut(s) | 905 |
| MmeI | TCCRAC | 2 cut(s) | 1739, 1815 |
| Mox20I | TGGCCA | 1 cut(s) | 34 |
| MroXI | GAANNNNTTC | 1 cut(s) | 601 |
| MscI | TGGCCA | 1 cut(s) | 34 |
| MseI | TTAA | 6 cut(s) | 57, 644, 1170, 1214, 1469, 1870 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1043, 1773 |
| Msp20I | TGGCCA | 1 cut(s) | 34 |
| MspA1I | CMGCKG | 3 cut(s) | 228, 1060, 1273 |
| MspI | CCGG | 3 cut(s) | 232, 342, 1663 |
| MspR9I | CCNGG | 4 cut(s) | 15, 79, 1220, 1663 |
| MunI | CAATTG | 1 cut(s) | 1389 |
| Mva1269I | GAATGC | 1 cut(s) | 18 |
| MvaI | CCWGG | 3 cut(s) | 15, 79, 1220 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 80, 947, 1626, 1725 |
| NciI | CCSGG | 1 cut(s) | 1663 |
| NcoI | CCATGG | 3 cut(s) | 35, 816, 1124 |
| NdeII | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| NlaIV | GGNNCC | 1 cut(s) | 814 |
| NmuCI | GTSAC | 2 cut(s) | 337, 499 |
| NsbI | TGCGCA | 1 cut(s) | 1619 |
| NspI | RCATGY | 2 cut(s) | 982, 1748 |
| NspV | TTCGAA | 2 cut(s) | 258, 766 |
| OliI | CACNNNNGTG | 1 cut(s) | 1043 |
| PaeI | GCATGC | 1 cut(s) | 1748 |
| PasI | CCCWGGG | 1 cut(s) | 78 |
| PciI | ACATGT | 1 cut(s) | 978 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1788 |
| PctI | GAATGC | 1 cut(s) | 18 |
| PdmI | GAANNNNTTC | 1 cut(s) | 601 |
| PfeI | GAWTC | 3 cut(s) | 378, 845, 1843 |
| PkrI | GCNGC | 6 cut(s) | 177, 227, 230, 329, 960, 1059 |
| PleI | GAGTC | 1 cut(s) | 905 |
| PmaCI | CACGTG | 1 cut(s) | 337 |
| PmlI | CACGTG | 1 cut(s) | 337 |
| PpsI | GAGTC | 1 cut(s) | 905 |
| Ppu21I | YACGTR | 2 cut(s) | 64, 337 |
| PscI | ACATGT | 1 cut(s) | 978 |
| PshAI | GACNNNNGTC | 1 cut(s) | 246 |
| PsiI | TTATAA | 1 cut(s) | 386 |
| Psp124BI | GAGCTC | 2 cut(s) | 445, 678 |
| Psp6I | CCWGG | 3 cut(s) | 13, 77, 1218 |
| PspCI | CACGTG | 1 cut(s) | 337 |
| PspGI | CCWGG | 3 cut(s) | 13, 77, 1218 |
| PspN4I | GGNNCC | 1 cut(s) | 814 |
| PspPI | GGNCC | 2 cut(s) | 407, 690 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1626, 1840 |
| PsuI | RGATCY | 3 cut(s) | 130, 192, 1656 |
| PvuII | CAGCTG | 1 cut(s) | 1273 |
| RsaI | GTAC | 4 cut(s) | 62, 578, 740, 977 |
| RsaNI | GTAC | 4 cut(s) | 61, 577, 739, 976 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1043, 1773 |
| SacI | GAGCTC | 2 cut(s) | 445, 678 |
| SaqAI | TTAA | 6 cut(s) | 57, 644, 1170, 1214, 1469, 1870 |
| SatI | GCNGC | 6 cut(s) | 176, 226, 229, 328, 959, 1058 |
| Sau3AI | GATC | 5 cut(s) | 130, 192, 234, 1092, 1656 |
| Sau96I | GGNCC | 2 cut(s) | 407, 690 |
| SchI | GAGTC | 1 cut(s) | 905 |
| ScrFI | CCNGG | 4 cut(s) | 15, 79, 1220, 1663 |
| SduI | GDGCHC | 4 cut(s) | 445, 543, 678, 1212 |
| SfaNI | GCATC | 4 cut(s) | 162, 760, 895, 1005 |
| SfuI | TTCGAA | 2 cut(s) | 258, 766 |
| SinI | GGWCC | 2 cut(s) | 407, 690 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1043, 1773 |
| SmlI | CTYRAG | 2 cut(s) | 713, 1670 |
| SmoI | CTYRAG | 2 cut(s) | 713, 1670 |
| SpeI | ACTAGT | 1 cut(s) | 827 |
| SphI | GCATGC | 1 cut(s) | 1748 |
| SsiI | CCGC | 3 cut(s) | 228, 959, 1060 |
| SspI | AATATT | 1 cut(s) | 1868 |
| SspMI | CTAG | 3 cut(s) | 251, 323, 828 |
| SstI | GAGCTC | 2 cut(s) | 445, 678 |
| StyD4I | CCNGG | 4 cut(s) | 13, 77, 1218, 1661 |
| StyI | CCWWGG | 6 cut(s) | 35, 322, 816, 945, 1124, 1749 |
| TaaI | ACNGT | 1 cut(s) | 840 |
| TaiI | ACGT | 2 cut(s) | 66, 339 |
| TaqI | TCGA | 9 cut(s) | 205, 240, 258, 349, 672, 766, 1010, 1797, 1822 |
| TatI | WGTACW | 1 cut(s) | 576 |
| TauI | GCSGC | 2 cut(s) | 231, 961 |
| TfiI | GAWTC | 3 cut(s) | 378, 845, 1843 |
| Tru1I | TTAA | 6 cut(s) | 57, 644, 1170, 1214, 1469, 1870 |
| Tru9I | TTAA | 6 cut(s) | 57, 644, 1170, 1214, 1469, 1870 |
| TscAI | CASTG | 4 cut(s) | 417, 508, 668, 1643 |
| TseFI | GTSAC | 2 cut(s) | 337, 499 |
| TseI | GCWGC | 4 cut(s) | 175, 225, 327, 1057 |
| Tsp45I | GTSAC | 2 cut(s) | 337, 499 |
| TspRI | CASTG | 4 cut(s) | 417, 508, 668, 1643 |
| VpaK11BI | GGWCC | 2 cut(s) | 407, 690 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| XbaI | TCTAGA | 1 cut(s) | 250 |
| XceI | RCATGY | 2 cut(s) | 982, 1748 |
| XmaJI | CCTAGG | 1 cut(s) | 322 |
| XmnI | GAANNNNTTC | 1 cut(s) | 601 |
| XspI | CTAG | 3 cut(s) | 251, 323, 828 |