Belongs to the sterol desaturase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1196 |
| AarI | CACCTGC | 1 cut(s) | 315 |
| Acc36I | ACCTGC | 2 cut(s) | 315, 1122 |
| Acc65I | GGTACC | 1 cut(s) | 812 |
| AccB1I | GGYRCC | 1 cut(s) | 812 |
| AciI | CCGC | 2 cut(s) | 1601, 1848 |
| AclWI | GGATC | 6 cut(s) | 197, 698, 1530, 1543, 1648, 1834 |
| AcoI | YGGCCR | 3 cut(s) | 32, 989, 1030 |
| AcsI | RAATTY | 3 cut(s) | 18, 251, 306 |
| AcyI | GRCGYC | 1 cut(s) | 1158 |
| AfaI | GTAC | 7 cut(s) | 75, 419, 769, 814, 974, 1112, 1639 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 877, 969, 1372 |
| AflII | CTTAAG | 1 cut(s) | 713 |
| AgsI | TTSAA | 3 cut(s) | 301, 1169, 1450 |
| AjnI | CCWGG | 3 cut(s) | 13, 1344, 1416 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1087, 1119 |
| AluBI | AGCT | 9 cut(s) | 54, 602, 1136, 1172, 1299, 1453, 1503, 1730, 1811 |
| AluI | AGCT | 9 cut(s) | 54, 602, 1136, 1172, 1299, 1453, 1503, 1730, 1811 |
| Alw21I | GWGCWC | 3 cut(s) | 445, 543, 1564 |
| Alw26I | GTCTC | 4 cut(s) | 348, 1045, 1211, 1668 |
| Alw44I | GTGCAC | 1 cut(s) | 1560 |
| AlwI | GGATC | 6 cut(s) | 197, 698, 1530, 1543, 1648, 1834 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1114 |
| AoxI | GGCC | 5 cut(s) | 32, 945, 989, 1030, 1225 |
| ApaLI | GTGCAC | 1 cut(s) | 1560 |
| ApeKI | GCWGC | 1 cut(s) | 394 |
| ApoI | RAATTY | 3 cut(s) | 18, 251, 306 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 601 |
| Asp718I | GGTACC | 1 cut(s) | 812 |
| AspLEI | GCGC | 1 cut(s) | 1340 |
| AspS9I | GGNCC | 3 cut(s) | 331, 407, 1225 |
| AsuHPI | GGTGA | 7 cut(s) | 94, 440, 476, 518, 1253, 1447, 1720 |
| AsuII | TTCGAA | 1 cut(s) | 255 |
| AvaII | GGWCC | 2 cut(s) | 331, 407 |
| BaeGI | GKGCMC | 1 cut(s) | 1564 |
| BalI | TGGCCA | 2 cut(s) | 34, 991 |
| BamHI | GGATCC | 1 cut(s) | 1535 |
| BanI | GGYRCC | 1 cut(s) | 812 |
| Bbv12I | GWGCWC | 3 cut(s) | 445, 543, 1564 |
| BbvI | GCAGC | 1 cut(s) | 381 |
| BccI | CCATC | 4 cut(s) | 614, 920, 1283, 1526 |
| BceAI | ACGGC | 2 cut(s) | 1017, 1283 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 235, 269 |
| BciT130I | CCWGG | 3 cut(s) | 15, 1346, 1418 |
| BclI | TGATCA | 1 cut(s) | 286 |
| BcoDI | GTCTC | 4 cut(s) | 348, 1045, 1211, 1668 |
| BfaI | CTAG | 2 cut(s) | 339, 828 |
| BfmI | CTRYAG | 1 cut(s) | 1473 |
| BfoI | RGCGCY | 1 cut(s) | 1341 |
| BfrI | CTTAAG | 1 cut(s) | 713 |
| BfuAI | ACCTGC | 2 cut(s) | 315, 1122 |
| BglII | AGATCT | 1 cut(s) | 127 |
| BisI | GCNGC | 1 cut(s) | 395 |
| BlsI | GCNGC | 1 cut(s) | 396 |
| Bme1390I | CCNGG | 3 cut(s) | 15, 1346, 1418 |
| Bme18I | GGWCC | 2 cut(s) | 331, 407 |
| BmgT120I | GGNCC | 3 cut(s) | 331, 407, 1225 |
| BmiI | GGNNCC | 3 cut(s) | 332, 814, 1537 |
| BmrFI | CCNGG | 3 cut(s) | 15, 1346, 1418 |
| BmsI | GCATC | 5 cut(s) | 159, 255, 381, 760, 1002 |
| BpmI | CTGGAG | 1 cut(s) | 1626 |
| Bpu14I | TTCGAA | 1 cut(s) | 255 |
| BpuEI | CTTGAG | 2 cut(s) | 839, 1688 |
| BsaBI | GATNNNNATC | 1 cut(s) | 188 |
| BsaHI | GRCGYC | 1 cut(s) | 1158 |
| BsaI | GGTCTC | 2 cut(s) | 348, 1211 |
| BsaJI | CCNNGG | 6 cut(s) | 35, 77, 816, 1265, 1344, 1700 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 476, 506 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 877, 969, 1372 |
| Bse1I | ACTGG | 6 cut(s) | 35, 434, 692, 992, 1539, 1697 |
| Bse8I | GATNNNNATC | 1 cut(s) | 188 |
| BseBI | CCWGG | 3 cut(s) | 15, 1346, 1418 |
| BseDI | CCNNGG | 6 cut(s) | 35, 77, 816, 1265, 1344, 1700 |
| BseGI | GGATG | 6 cut(s) | 138, 174, 289, 611, 1294, 1314 |
| BseJI | GATNNNNATC | 1 cut(s) | 188 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 877, 969, 1372 |
| BseMII | CTCAG | 4 cut(s) | 359, 495, 1164, 1431 |
| BseNI | ACTGG | 6 cut(s) | 35, 434, 692, 992, 1539, 1697 |
| BseRI | GAGGAG | 1 cut(s) | 699 |
| BseSI | GKGCMC | 1 cut(s) | 1564 |
| BseXI | GCAGC | 1 cut(s) | 381 |
| BshFI | GGCC | 5 cut(s) | 34, 947, 991, 1032, 1227 |
| BshNI | GGYRCC | 1 cut(s) | 812 |
| BsiHKAI | GWGCWC | 3 cut(s) | 445, 543, 1564 |
| BslFI | GGGAC | 3 cut(s) | 241, 672, 1169 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 877, 969, 1372 |
| BsmAI | GTCTC | 4 cut(s) | 348, 1045, 1211, 1668 |
| BsmFI | GGGAC | 3 cut(s) | 241, 672, 1169 |
| BsmI | GAATGC | 1 cut(s) | 1326 |
| BsnI | GGCC | 5 cut(s) | 34, 947, 991, 1032, 1227 |
| Bso31I | GGTCTC | 2 cut(s) | 348, 1211 |
| Bsp119I | TTCGAA | 1 cut(s) | 255 |
| Bsp1286I | GDGCHC | 3 cut(s) | 445, 543, 1564 |
| Bsp143I | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| Bsp19I | CCATGG | 2 cut(s) | 35, 816 |
| BspACI | CCGC | 2 cut(s) | 1601, 1848 |
| BspANI | GGCC | 5 cut(s) | 34, 947, 991, 1032, 1227 |
| BspCNI | CTCAG | 4 cut(s) | 358, 496, 1165, 1432 |
| BspHI | TCATGA | 3 cut(s) | 384, 645, 1492 |
| BspLI | GGNNCC | 3 cut(s) | 332, 814, 1537 |
| BspMI | ACCTGC | 2 cut(s) | 315, 1122 |
| BspPI | GGATC | 6 cut(s) | 197, 698, 1530, 1543, 1648, 1834 |
| BspQI | GCTCTTC | 1 cut(s) | 566 |
| BspT104I | TTCGAA | 1 cut(s) | 255 |
| BspT107I | GGYRCC | 1 cut(s) | 812 |
| BspTI | CTTAAG | 1 cut(s) | 713 |
| BspTNI | GGTCTC | 2 cut(s) | 348, 1211 |
| BsrI | ACTGG | 6 cut(s) | 35, 434, 692, 992, 1539, 1697 |
| BssECI | CCNNGG | 6 cut(s) | 35, 77, 816, 1265, 1344, 1700 |
| BssMI | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| BssNI | GRCGYC | 1 cut(s) | 1158 |
| BssT1I | CCWWGG | 4 cut(s) | 35, 77, 816, 1700 |
| Bst2UI | CCWGG | 3 cut(s) | 15, 1346, 1418 |
| Bst4CI | ACNGT | 4 cut(s) | 1103, 1554, 1625, 1637 |
| Bst6I | CTCTTC | 3 cut(s) | 566, 788, 1821 |
| BstACI | GRCGYC | 1 cut(s) | 1158 |
| BstAFI | CTTAAG | 1 cut(s) | 713 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1114 |
| BstBI | TTCGAA | 1 cut(s) | 255 |
| BstC8I | GCNNGC | 3 cut(s) | 1437, 1743, 1809 |
| BstDEI | CTNAG | 5 cut(s) | 345, 504, 906, 1173, 1440 |
| BstDSI | CCRYGG | 3 cut(s) | 35, 816, 1265 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1370 |
| BstF5I | GGATG | 6 cut(s) | 138, 174, 289, 611, 1294, 1314 |
| BstH2I | RGCGCY | 1 cut(s) | 1341 |
| BstHHI | GCGC | 1 cut(s) | 1340 |
| BstKTI | GATC | 7 cut(s) | 130, 192, 289, 693, 1538, 1656, 1842 |
| BstMAI | GTCTC | 4 cut(s) | 348, 1045, 1211, 1668 |
| BstMBI | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 944, 1114, 1123 |
| BstNI | CCWGG | 3 cut(s) | 15, 1346, 1418 |
| BstNSI | RCATGY | 1 cut(s) | 1745 |
| BstSCI | CCNGG | 3 cut(s) | 13, 1344, 1416 |
| BstSFI | CTRYAG | 1 cut(s) | 1473 |
| BstSLI | GKGCMC | 1 cut(s) | 1564 |
| BstV1I | GCAGC | 1 cut(s) | 381 |
| BstX2I | RGATCY | 5 cut(s) | 127, 189, 690, 1535, 1653 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1772 |
| BstYI | RGATCY | 5 cut(s) | 127, 189, 690, 1535, 1653 |
| BsuRI | GGCC | 5 cut(s) | 34, 947, 991, 1032, 1227 |
| BtgI | CCRYGG | 3 cut(s) | 35, 816, 1265 |
| BtsCI | GGATG | 6 cut(s) | 138, 174, 289, 611, 1294, 1314 |
| BtsI | GCAGTG | 1 cut(s) | 661 |
| BtsIMutI | CAGTG | 5 cut(s) | 501, 661, 1546, 1633, 1835 |
| BveI | ACCTGC | 2 cut(s) | 315, 1122 |
| Cac8I | GCNNGC | 3 cut(s) | 1437, 1743, 1809 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1114 |
| CciI | TCATGA | 3 cut(s) | 384, 645, 1492 |
| CfoI | GCGC | 1 cut(s) | 1340 |
| Cfr13I | GGNCC | 3 cut(s) | 331, 407, 1225 |
| CseI | GACGC | 1 cut(s) | 1166 |
| Csp6I | GTAC | 7 cut(s) | 74, 418, 768, 813, 973, 1111, 1638 |
| CviQI | GTAC | 7 cut(s) | 74, 418, 768, 813, 973, 1111, 1638 |
| DdeI | CTNAG | 5 cut(s) | 345, 504, 906, 1173, 1440 |
| DpnI | GATC | 7 cut(s) | 129, 191, 288, 692, 1537, 1655, 1841 |
| DpnII | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| EaeI | YGGCCR | 3 cut(s) | 32, 989, 1030 |
| Eam1104I | CTCTTC | 3 cut(s) | 566, 788, 1821 |
| EarI | CTCTTC | 3 cut(s) | 566, 788, 1821 |
| Eco130I | CCWWGG | 4 cut(s) | 35, 77, 816, 1700 |
| Eco31I | GGTCTC | 2 cut(s) | 348, 1211 |
| Eco32I | GATATC | 1 cut(s) | 1414 |
| Eco47I | GGWCC | 2 cut(s) | 331, 407 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1370 |
| EcoO109I | RGGNCCY | 2 cut(s) | 331, 1225 |
| EcoRI | GAATTC | 3 cut(s) | 18, 251, 306 |
| EcoRII | CCWGG | 3 cut(s) | 13, 1344, 1416 |
| EcoRV | GATATC | 1 cut(s) | 1414 |
| EcoT14I | CCWWGG | 4 cut(s) | 35, 77, 816, 1700 |
| EcoT22I | ATGCAT | 1 cut(s) | 1747 |
| ErhI | CCWWGG | 4 cut(s) | 35, 77, 816, 1700 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1800, 1832 |
| FaqI | GGGAC | 3 cut(s) | 241, 672, 1169 |
| FauI | CCCGC | 1 cut(s) | 1608 |
| FbaI | TGATCA | 1 cut(s) | 286 |
| Fnu4HI | GCNGC | 1 cut(s) | 395 |
| FokI | GGATG | 6 cut(s) | 125, 181, 296, 598, 1301, 1301 |
| Fsp4HI | GCNGC | 1 cut(s) | 395 |
| FspBI | CTAG | 2 cut(s) | 339, 828 |
| GlaI | GCGC | 1 cut(s) | 1339 |
| GluI | GCNGC | 1 cut(s) | 395 |
| GsuI | CTGGAG | 1 cut(s) | 1626 |
| HaeII | RGCGCY | 1 cut(s) | 1341 |
| HaeIII | GGCC | 5 cut(s) | 34, 947, 991, 1032, 1227 |
| HgaI | GACGC | 1 cut(s) | 1166 |
| HhaI | GCGC | 1 cut(s) | 1340 |
| Hin1I | GRCGYC | 1 cut(s) | 1158 |
| Hin6I | GCGC | 1 cut(s) | 1338 |
| HinP1I | GCGC | 1 cut(s) | 1338 |
| HinfI | GANTC | 6 cut(s) | 85, 523, 845, 866, 921, 1261 |
| HphI | GGTGA | 7 cut(s) | 94, 440, 476, 518, 1253, 1447, 1720 |
| Hpy166II | GTNNAC | 2 cut(s) | 1562, 1678 |
| Hpy188I | TCNGA | 6 cut(s) | 348, 411, 596, 684, 1023, 1789 |
| Hpy188III | TCNNGA | 7 cut(s) | 334, 385, 646, 856, 1255, 1493, 1667 |
| Hpy8I | GTNNAC | 2 cut(s) | 1562, 1678 |
| HpyAV | CCTTC | 3 cut(s) | 113, 1597, 1745 |
| HpyCH4III | ACNGT | 4 cut(s) | 1103, 1554, 1625, 1637 |
| HpyCH4V | TGCA | 6 cut(s) | 536, 666, 751, 1562, 1741, 1745 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 944, 1114, 1123 |
| HpyF3I | CTNAG | 5 cut(s) | 345, 504, 906, 1173, 1440 |
| Hsp92I | GRCGYC | 1 cut(s) | 1158 |
| HspAI | GCGC | 1 cut(s) | 1338 |
| KpnI | GGTACC | 1 cut(s) | 816 |
| Ksp22I | TGATCA | 1 cut(s) | 286 |
| Kzo9I | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| LguI | GCTCTTC | 1 cut(s) | 566 |
| LmnI | GCTCC | 2 cut(s) | 51, 177 |
| Lsp1109I | GCAGC | 1 cut(s) | 381 |
| LweI | GCATC | 5 cut(s) | 159, 255, 381, 760, 1002 |
| MaeI | CTAG | 2 cut(s) | 339, 828 |
| MaeIII | GTNAC | 6 cut(s) | 432, 499, 520, 994, 1423, 1625 |
| MalI | GATC | 7 cut(s) | 129, 191, 288, 692, 1537, 1655, 1841 |
| MboI | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| MboII | GAAGA | 7 cut(s) | 128, 553, 685, 775, 840, 1663, 1808 |
| MflI | RGATCY | 5 cut(s) | 127, 189, 690, 1535, 1653 |
| MhlI | GDGCHC | 3 cut(s) | 445, 543, 1564 |
| MlsI | TGGCCA | 2 cut(s) | 34, 991 |
| MluNI | TGGCCA | 2 cut(s) | 34, 991 |
| MlyI | GAGTC | 2 cut(s) | 517, 915 |
| MmeI | TCCRAC | 1 cut(s) | 1736 |
| Mox20I | TGGCCA | 2 cut(s) | 34, 991 |
| Mph1103I | ATGCAT | 1 cut(s) | 1747 |
| MroXI | GAANNNNTTC | 1 cut(s) | 601 |
| MscI | TGGCCA | 2 cut(s) | 34, 991 |
| MseI | TTAA | 9 cut(s) | 57, 590, 714, 896, 1212, 1230, 1320, 1466, 1858 |
| MslI | CAYNNNNRTG | 2 cut(s) | 389, 1770 |
| Msp20I | TGGCCA | 2 cut(s) | 34, 991 |
| MspCI | CTTAAG | 1 cut(s) | 713 |
| MspR9I | CCNGG | 3 cut(s) | 15, 1346, 1418 |
| Mva1269I | GAATGC | 1 cut(s) | 1326 |
| MvaI | CCWGG | 3 cut(s) | 15, 1346, 1418 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 944, 1114, 1123 |
| NcoI | CCATGG | 2 cut(s) | 35, 816 |
| NdeII | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| NlaIV | GGNNCC | 3 cut(s) | 332, 814, 1537 |
| NmuCI | GTSAC | 2 cut(s) | 499, 520 |
| NsiI | ATGCAT | 1 cut(s) | 1747 |
| NspI | RCATGY | 1 cut(s) | 1745 |
| NspV | TTCGAA | 1 cut(s) | 255 |
| PaeI | GCATGC | 1 cut(s) | 1745 |
| PagI | TCATGA | 3 cut(s) | 384, 645, 1492 |
| PaqCI | CACCTGC | 1 cut(s) | 315 |
| PciSI | GCTCTTC | 1 cut(s) | 566 |
| PctI | GAATGC | 1 cut(s) | 1326 |
| PdmI | GAANNNNTTC | 1 cut(s) | 601 |
| PfeI | GAWTC | 4 cut(s) | 85, 845, 866, 1261 |
| PflFI | GACNNNGTC | 1 cut(s) | 340 |
| PkrI | GCNGC | 1 cut(s) | 396 |
| PleI | GAGTC | 2 cut(s) | 517, 915 |
| PpsI | GAGTC | 2 cut(s) | 517, 915 |
| PpuMI | RGGWCCY | 1 cut(s) | 331 |
| PsiI | TTATAA | 1 cut(s) | 1196 |
| Psp5II | RGGWCCY | 1 cut(s) | 331 |
| Psp6I | CCWGG | 3 cut(s) | 13, 1344, 1416 |
| PspGI | CCWGG | 3 cut(s) | 13, 1344, 1416 |
| PspN4I | GGNNCC | 3 cut(s) | 332, 814, 1537 |
| PspPI | GGNCC | 3 cut(s) | 331, 407, 1225 |
| PspPPI | RGGWCCY | 1 cut(s) | 331 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1114 |
| PsuI | RGATCY | 5 cut(s) | 127, 189, 690, 1535, 1653 |
| PsyI | GACNNNGTC | 1 cut(s) | 340 |
| RsaI | GTAC | 7 cut(s) | 75, 419, 769, 814, 974, 1112, 1639 |
| RsaNI | GTAC | 7 cut(s) | 74, 418, 768, 813, 973, 1111, 1638 |
| RseI | CAYNNNNRTG | 2 cut(s) | 389, 1770 |
| SapI | GCTCTTC | 1 cut(s) | 566 |
| SaqAI | TTAA | 9 cut(s) | 57, 590, 714, 896, 1212, 1230, 1320, 1466, 1858 |
| SatI | GCNGC | 1 cut(s) | 395 |
| Sau3AI | GATC | 7 cut(s) | 127, 189, 286, 690, 1535, 1653, 1839 |
| Sau96I | GGNCC | 3 cut(s) | 331, 407, 1225 |
| SchI | GAGTC | 2 cut(s) | 517, 915 |
| ScrFI | CCNGG | 3 cut(s) | 15, 1346, 1418 |
| SduI | GDGCHC | 3 cut(s) | 445, 543, 1564 |
| SfaNI | GCATC | 5 cut(s) | 159, 255, 381, 760, 1002 |
| SfcI | CTRYAG | 1 cut(s) | 1473 |
| SfuI | TTCGAA | 1 cut(s) | 255 |
| SinI | GGWCC | 2 cut(s) | 331, 407 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 389, 1770 |
| SmlI | CTYRAG | 3 cut(s) | 713, 854, 1667 |
| SmoI | CTYRAG | 3 cut(s) | 713, 854, 1667 |
| SphI | GCATGC | 1 cut(s) | 1745 |
| SsiI | CCGC | 2 cut(s) | 1601, 1848 |
| SspI | AATATT | 1 cut(s) | 1578 |
| SspMI | CTAG | 2 cut(s) | 339, 828 |
| StyD4I | CCNGG | 3 cut(s) | 13, 1344, 1416 |
| StyI | CCWWGG | 4 cut(s) | 35, 77, 816, 1700 |
| TaaI | ACNGT | 4 cut(s) | 1103, 1554, 1625, 1637 |
| TaqI | TCGA | 6 cut(s) | 164, 249, 255, 639, 672, 1819 |
| TatI | WGTACW | 1 cut(s) | 1637 |
| TfiI | GAWTC | 4 cut(s) | 85, 845, 866, 1261 |
| Tru1I | TTAA | 9 cut(s) | 57, 590, 714, 896, 1212, 1230, 1320, 1466, 1858 |
| Tru9I | TTAA | 9 cut(s) | 57, 590, 714, 896, 1212, 1230, 1320, 1466, 1858 |
| TscAI | CASTG | 5 cut(s) | 508, 668, 1546, 1640, 1842 |
| TseFI | GTSAC | 2 cut(s) | 499, 520 |
| TseI | GCWGC | 1 cut(s) | 394 |
| Tsp45I | GTSAC | 2 cut(s) | 499, 520 |
| TspGWI | ACGGA | 2 cut(s) | 318, 1584 |
| TspRI | CASTG | 5 cut(s) | 508, 668, 1546, 1640, 1842 |
| Tth111I | GACNNNGTC | 1 cut(s) | 340 |
| Vha464I | CTTAAG | 1 cut(s) | 713 |
| VneI | GTGCAC | 1 cut(s) | 1560 |
| VpaK11BI | GGWCC | 2 cut(s) | 331, 407 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1370 |
| XapI | RAATTY | 3 cut(s) | 18, 251, 306 |
| XceI | RCATGY | 1 cut(s) | 1745 |
| XmnI | GAANNNNTTC | 1 cut(s) | 601 |
| XspI | CTAG | 2 cut(s) | 339, 828 |
| Zsp2I | ATGCAT | 1 cut(s) | 1747 |