Belongs to the sterol desaturase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1607 |
| Acc65I | GGTACC | 1 cut(s) | 803 |
| AccB1I | GGYRCC | 1 cut(s) | 803 |
| AccI | GTMKAC | 4 cut(s) | 261, 719, 1066, 1668 |
| AciI | CCGC | 1 cut(s) | 527 |
| AclWI | GGATC | 4 cut(s) | 135, 197, 689, 1639 |
| AcoI | YGGCCR | 3 cut(s) | 32, 980, 1021 |
| AcsI | RAATTY | 3 cut(s) | 18, 351, 1088 |
| AcuI | CTGAAG | 2 cut(s) | 320, 1283 |
| AfaI | GTAC | 7 cut(s) | 62, 124, 731, 805, 965, 1103, 1436 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 526, 569, 1363 |
| AflII | CTTAAG | 1 cut(s) | 704 |
| AflIII | ACRYGT | 1 cut(s) | 795 |
| AgsI | TTSAA | 3 cut(s) | 298, 1160, 1441 |
| AjnI | CCWGG | 2 cut(s) | 1335, 1425 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 668, 700 |
| AleI | CACNNNNGTG | 2 cut(s) | 509, 1761 |
| AluBI | AGCT | 9 cut(s) | 54, 74, 896, 1127, 1163, 1290, 1295, 1444, 1494 |
| AluI | AGCT | 9 cut(s) | 54, 74, 896, 1127, 1163, 1290, 1295, 1444, 1494 |
| Alw21I | GWGCWC | 4 cut(s) | 436, 534, 1555, 1758 |
| Alw26I | GTCTC | 2 cut(s) | 1605, 1659 |
| Alw44I | GTGCAC | 1 cut(s) | 1551 |
| AlwI | GGATC | 4 cut(s) | 135, 197, 689, 1639 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1614 |
| Ama87I | CYCGRG | 1 cut(s) | 245 |
| AoxI | GGCC | 5 cut(s) | 32, 241, 936, 980, 1021 |
| ApaLI | GTGCAC | 1 cut(s) | 1551 |
| ApeKI | GCWGC | 1 cut(s) | 1045 |
| ApoI | RAATTY | 3 cut(s) | 18, 351, 1088 |
| AseI | ATTAAT | 1 cut(s) | 1001 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 592 |
| Asp718I | GGTACC | 1 cut(s) | 803 |
| AspLEI | GCGC | 1 cut(s) | 1608 |
| AspS9I | GGNCC | 2 cut(s) | 241, 398 |
| AsuC2I | CCSGG | 1 cut(s) | 15 |
| AsuHPI | GGTGA | 7 cut(s) | 94, 431, 467, 509, 852, 1223, 1438 |
| AsuII | TTCGAA | 2 cut(s) | 252, 757 |
| AvaI | CYCGRG | 1 cut(s) | 245 |
| AvaII | GGWCC | 1 cut(s) | 398 |
| BaeGI | GKGCMC | 1 cut(s) | 1555 |
| BalI | TGGCCA | 2 cut(s) | 34, 982 |
| BanI | GGYRCC | 1 cut(s) | 803 |
| BanII | GRGCYC | 1 cut(s) | 1221 |
| BauI | CACGAG | 1 cut(s) | 1757 |
| BbsI | GAAGAC | 1 cut(s) | 1190 |
| Bbv12I | GWGCWC | 4 cut(s) | 436, 534, 1555, 1758 |
| BbvI | GCAGC | 1 cut(s) | 1057 |
| BccI | CCATC | 6 cut(s) | 236, 359, 605, 911, 1274, 1517 |
| BceAI | ACGGC | 2 cut(s) | 606, 1008 |
| BciT130I | CCWGG | 2 cut(s) | 1337, 1427 |
| BclI | TGATCA | 1 cut(s) | 283 |
| BcnI | CCSGG | 1 cut(s) | 15 |
| BcoDI | GTCTC | 2 cut(s) | 1605, 1659 |
| BfaI | CTAG | 1 cut(s) | 819 |
| BfrI | CTTAAG | 1 cut(s) | 704 |
| BisI | GCNGC | 1 cut(s) | 1046 |
| BlpI | GCTNAGC | 2 cut(s) | 1164, 1291 |
| BlsI | GCNGC | 1 cut(s) | 1047 |
| BmcAI | AGTACT | 2 cut(s) | 1103, 1436 |
| Bme1390I | CCNGG | 3 cut(s) | 15, 1337, 1427 |
| Bme18I | GGWCC | 1 cut(s) | 398 |
| BmeT110I | CYCGRG | 1 cut(s) | 245 |
| BmgT120I | GGNCC | 2 cut(s) | 241, 398 |
| BmiI | GGNNCC | 1 cut(s) | 805 |
| BmrFI | CCNGG | 3 cut(s) | 15, 1337, 1427 |
| BmsI | GCATC | 5 cut(s) | 159, 314, 336, 751, 1138 |
| BpiI | GAAGAC | 1 cut(s) | 1190 |
| BpmI | CTGGAG | 1 cut(s) | 1679 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1164, 1291 |
| Bpu14I | TTCGAA | 2 cut(s) | 252, 757 |
| BpuMI | CCSGG | 1 cut(s) | 15 |
| BsaAI | YACGTR | 2 cut(s) | 64, 316 |
| BsaBI | GATNNNNATC | 1 cut(s) | 188 |
| BsaJI | CCNNGG | 6 cut(s) | 35, 77, 807, 933, 1335, 1691 |
| BsaWI | WCCGGW | 1 cut(s) | 400 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 467, 497 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 526, 569, 1363 |
| Bse1I | ACTGG | 6 cut(s) | 35, 425, 683, 924, 983, 1688 |
| Bse3DI | GCAATG | 2 cut(s) | 1644, 1743 |
| Bse8I | GATNNNNATC | 1 cut(s) | 188 |
| BseBI | CCWGG | 2 cut(s) | 1337, 1427 |
| BseDI | CCNNGG | 6 cut(s) | 35, 77, 807, 933, 1335, 1691 |
| BseGI | GGATG | 8 cut(s) | 138, 174, 286, 327, 602, 1153, 1285, 1305 |
| BseJI | GATNNNNATC | 1 cut(s) | 188 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 526, 569, 1363 |
| BseMI | GCAATG | 2 cut(s) | 1644, 1743 |
| BseMII | CTCAG | 3 cut(s) | 486, 1155, 1422 |
| BseNI | ACTGG | 6 cut(s) | 35, 425, 683, 924, 983, 1688 |
| BseRI | GAGGAG | 3 cut(s) | 690, 869, 1708 |
| BseSI | GKGCMC | 1 cut(s) | 1555 |
| BseXI | GCAGC | 1 cut(s) | 1057 |
| Bsh1285I | CGRYCG | 1 cut(s) | 405 |
| BshFI | GGCC | 5 cut(s) | 34, 243, 938, 982, 1023 |
| BshNI | GGYRCC | 1 cut(s) | 803 |
| BsiEI | CGRYCG | 1 cut(s) | 405 |
| BsiHKAI | GWGCWC | 4 cut(s) | 436, 534, 1555, 1758 |
| BsiHKCI | CYCGRG | 1 cut(s) | 245 |
| BsiSI | CCGG | 2 cut(s) | 15, 401 |
| BslFI | GGGAC | 1 cut(s) | 663 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 526, 569, 1363 |
| BsmAI | GTCTC | 2 cut(s) | 1605, 1659 |
| BsmFI | GGGAC | 1 cut(s) | 663 |
| BsmI | GAATGC | 2 cut(s) | 309, 1317 |
| BsnI | GGCC | 5 cut(s) | 34, 243, 938, 982, 1023 |
| BsoBI | CYCGRG | 1 cut(s) | 245 |
| Bsp119I | TTCGAA | 2 cut(s) | 252, 757 |
| Bsp1286I | GDGCHC | 5 cut(s) | 436, 534, 1221, 1555, 1758 |
| Bsp143I | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1164, 1291 |
| Bsp19I | CCATGG | 2 cut(s) | 35, 807 |
| BspACI | CCGC | 1 cut(s) | 527 |
| BspANI | GGCC | 5 cut(s) | 34, 243, 938, 982, 1023 |
| BspCNI | CTCAG | 3 cut(s) | 487, 1156, 1423 |
| BspHI | TCATGA | 3 cut(s) | 375, 636, 1483 |
| BspLI | GGNNCC | 1 cut(s) | 805 |
| BspPI | GGATC | 4 cut(s) | 135, 197, 689, 1639 |
| BspQI | GCTCTTC | 1 cut(s) | 557 |
| BspT104I | TTCGAA | 2 cut(s) | 252, 757 |
| BspT107I | GGYRCC | 1 cut(s) | 803 |
| BspTI | CTTAAG | 1 cut(s) | 704 |
| BsrDI | GCAATG | 2 cut(s) | 1644, 1743 |
| BsrI | ACTGG | 6 cut(s) | 35, 425, 683, 924, 983, 1688 |
| BssECI | CCNNGG | 6 cut(s) | 35, 77, 807, 933, 1335, 1691 |
| BssMI | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| BssNAI | GTATAC | 1 cut(s) | 720 |
| BssSI | CACGAG | 1 cut(s) | 1757 |
| BssT1I | CCWWGG | 4 cut(s) | 35, 77, 807, 933 |
| Bst1107I | GTATAC | 1 cut(s) | 720 |
| Bst2BI | CACGAG | 1 cut(s) | 1757 |
| Bst2UI | CCWGG | 2 cut(s) | 1337, 1427 |
| Bst4CI | ACNGT | 2 cut(s) | 1064, 1545 |
| Bst6I | CTCTTC | 3 cut(s) | 557, 779, 1812 |
| BstAFI | CTTAAG | 1 cut(s) | 704 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1614 |
| BstBAI | YACGTR | 2 cut(s) | 64, 316 |
| BstBI | TTCGAA | 2 cut(s) | 252, 757 |
| BstC8I | GCNNGC | 2 cut(s) | 1734, 1800 |
| BstDEI | CTNAG | 4 cut(s) | 495, 1164, 1291, 1431 |
| BstDSI | CCRYGG | 2 cut(s) | 35, 807 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1361 |
| BstF5I | GGATG | 8 cut(s) | 138, 174, 286, 327, 602, 1153, 1285, 1305 |
| BstHHI | GCGC | 1 cut(s) | 1608 |
| BstKTI | GATC | 9 cut(s) | 130, 192, 286, 291, 570, 585, 684, 1269, 1647 |
| BstMAI | GTCTC | 2 cut(s) | 1605, 1659 |
| BstMBI | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| BstMCI | CGRYCG | 1 cut(s) | 405 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 935, 979, 1105, 1114, 1614 |
| BstNI | CCWGG | 2 cut(s) | 1337, 1427 |
| BstNSI | RCATGY | 2 cut(s) | 799, 1736 |
| BstSCI | CCNGG | 3 cut(s) | 13, 1335, 1425 |
| BstSLI | GKGCMC | 1 cut(s) | 1555 |
| BstSNI | TACGTA | 1 cut(s) | 316 |
| BstV1I | GCAGC | 1 cut(s) | 1057 |
| BstV2I | GAAGAC | 1 cut(s) | 1190 |
| BstX2I | RGATCY | 4 cut(s) | 127, 189, 681, 1644 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1657 |
| BstYI | RGATCY | 4 cut(s) | 127, 189, 681, 1644 |
| BstZ17I | GTATAC | 1 cut(s) | 720 |
| BsuRI | GGCC | 5 cut(s) | 34, 243, 938, 982, 1023 |
| BtgI | CCRYGG | 2 cut(s) | 35, 807 |
| BtsCI | GGATG | 8 cut(s) | 138, 174, 286, 327, 602, 1153, 1285, 1305 |
| BtsI | GCAGTG | 1 cut(s) | 652 |
| BtsIMutI | CAGTG | 4 cut(s) | 492, 652, 917, 990 |
| Cac8I | GCNNGC | 2 cut(s) | 1734, 1800 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1614 |
| CciI | TCATGA | 3 cut(s) | 375, 636, 1483 |
| CfoI | GCGC | 1 cut(s) | 1608 |
| Cfr13I | GGNCC | 2 cut(s) | 241, 398 |
| Csp6I | GTAC | 7 cut(s) | 61, 123, 730, 804, 964, 1102, 1435 |
| CviQI | GTAC | 7 cut(s) | 61, 123, 730, 804, 964, 1102, 1435 |
| DdeI | CTNAG | 4 cut(s) | 495, 1164, 1291, 1431 |
| DpnI | GATC | 9 cut(s) | 129, 191, 285, 290, 569, 584, 683, 1268, 1646 |
| DpnII | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| EaeI | YGGCCR | 3 cut(s) | 32, 980, 1021 |
| Eam1104I | CTCTTC | 3 cut(s) | 557, 779, 1812 |
| EarI | CTCTTC | 3 cut(s) | 557, 779, 1812 |
| Eco105I | TACGTA | 1 cut(s) | 316 |
| Eco130I | CCWWGG | 4 cut(s) | 35, 77, 807, 933 |
| Eco24I | GRGCYC | 1 cut(s) | 1221 |
| Eco47I | GGWCC | 1 cut(s) | 398 |
| Eco57I | CTGAAG | 2 cut(s) | 320, 1283 |
| Eco88I | CYCGRG | 1 cut(s) | 245 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1361 |
| EcoO109I | RGGNCCY | 1 cut(s) | 241 |
| EcoRI | GAATTC | 1 cut(s) | 18 |
| EcoRII | CCWGG | 2 cut(s) | 1335, 1425 |
| EcoT14I | CCWWGG | 4 cut(s) | 35, 77, 807, 933 |
| EcoT22I | ATGCAT | 1 cut(s) | 329 |
| EcoT38I | GRGCYC | 1 cut(s) | 1221 |
| ErhI | CCWWGG | 4 cut(s) | 35, 77, 807, 933 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1791, 1823 |
| FaqI | GGGAC | 1 cut(s) | 663 |
| FbaI | TGATCA | 1 cut(s) | 283 |
| FblI | GTMKAC | 4 cut(s) | 261, 719, 1066, 1668 |
| Fnu4HI | GCNGC | 1 cut(s) | 1046 |
| FokI | GGATG | 8 cut(s) | 125, 181, 293, 314, 589, 1160, 1292, 1292 |
| FriOI | GRGCYC | 1 cut(s) | 1221 |
| Fsp4HI | GCNGC | 1 cut(s) | 1046 |
| FspBI | CTAG | 1 cut(s) | 819 |
| FspI | TGCGCA | 1 cut(s) | 1607 |
| GlaI | GCGC | 1 cut(s) | 1607 |
| GluI | GCNGC | 1 cut(s) | 1046 |
| GsuI | CTGGAG | 1 cut(s) | 1679 |
| HaeIII | GGCC | 5 cut(s) | 34, 243, 938, 982, 1023 |
| HapII | CCGG | 2 cut(s) | 15, 401 |
| HhaI | GCGC | 1 cut(s) | 1608 |
| Hin6I | GCGC | 1 cut(s) | 1606 |
| HinP1I | GCGC | 1 cut(s) | 1606 |
| HincII | GTYRAC | 1 cut(s) | 262 |
| HindII | GTYRAC | 1 cut(s) | 262 |
| HinfI | GANTC | 8 cut(s) | 85, 514, 824, 836, 857, 912, 1137, 1252 |
| HpaII | CCGG | 2 cut(s) | 15, 401 |
| HphI | GGTGA | 7 cut(s) | 94, 431, 467, 509, 852, 1223, 1438 |
| Hpy166II | GTNNAC | 5 cut(s) | 262, 720, 1067, 1553, 1669 |
| Hpy188I | TCNGA | 5 cut(s) | 288, 339, 572, 587, 1323 |
| Hpy188III | TCNNGA | 4 cut(s) | 376, 637, 1484, 1658 |
| Hpy8I | GTNNAC | 5 cut(s) | 262, 720, 1067, 1553, 1669 |
| HpyAV | CCTTC | 3 cut(s) | 113, 1588, 1736 |
| HpyCH4III | ACNGT | 2 cut(s) | 1064, 1545 |
| HpyCH4IV | ACGT | 2 cut(s) | 63, 315 |
| HpyCH4V | TGCA | 8 cut(s) | 215, 327, 657, 742, 1099, 1553, 1732, 1736 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 935, 979, 1105, 1114, 1614 |
| HpyF3I | CTNAG | 4 cut(s) | 495, 1164, 1291, 1431 |
| HpySE526I | ACGT | 2 cut(s) | 63, 315 |
| HspAI | GCGC | 1 cut(s) | 1606 |
| KpnI | GGTACC | 1 cut(s) | 807 |
| Ksp22I | TGATCA | 1 cut(s) | 283 |
| Kzo9I | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| LguI | GCTCTTC | 1 cut(s) | 557 |
| LmnI | GCTCC | 3 cut(s) | 51, 79, 529 |
| Lsp1109I | GCAGC | 1 cut(s) | 1057 |
| LweI | GCATC | 5 cut(s) | 159, 314, 336, 751, 1138 |
| MaeI | CTAG | 1 cut(s) | 819 |
| MaeII | ACGT | 2 cut(s) | 63, 315 |
| MaeIII | GTNAC | 7 cut(s) | 423, 490, 511, 858, 985, 1014, 1414 |
| MalI | GATC | 9 cut(s) | 129, 191, 285, 290, 569, 584, 683, 1268, 1646 |
| MboI | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| MboII | GAAGA | 9 cut(s) | 128, 544, 676, 766, 831, 1190, 1276, 1654, 1799 |
| MflI | RGATCY | 4 cut(s) | 127, 189, 681, 1644 |
| MhlI | GDGCHC | 5 cut(s) | 436, 534, 1221, 1555, 1758 |
| MlsI | TGGCCA | 2 cut(s) | 34, 982 |
| MluCI | AATT | 7 cut(s) | 18, 351, 658, 752, 763, 1088, 1586 |
| MluNI | TGGCCA | 2 cut(s) | 34, 982 |
| MlyI | GAGTC | 3 cut(s) | 508, 866, 906 |
| MmeI | TCCRAC | 2 cut(s) | 1727, 1803 |
| Mox20I | TGGCCA | 2 cut(s) | 34, 982 |
| Mph1103I | ATGCAT | 1 cut(s) | 329 |
| MroXI | GAANNNNTTC | 1 cut(s) | 592 |
| MscI | TGGCCA | 2 cut(s) | 34, 982 |
| MseI | TTAA | 7 cut(s) | 57, 705, 887, 1001, 1203, 1311, 1457 |
| MslI | CAYNNNNRTG | 2 cut(s) | 509, 1761 |
| Msp20I | TGGCCA | 2 cut(s) | 34, 982 |
| MspCI | CTTAAG | 1 cut(s) | 704 |
| MspI | CCGG | 2 cut(s) | 15, 401 |
| MspR9I | CCNGG | 3 cut(s) | 15, 1337, 1427 |
| Mva1269I | GAATGC | 2 cut(s) | 309, 1317 |
| MvaI | CCWGG | 2 cut(s) | 1337, 1427 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 935, 979, 1105, 1114, 1614 |
| NciI | CCSGG | 1 cut(s) | 15 |
| NcoI | CCATGG | 2 cut(s) | 35, 807 |
| NdeII | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| NlaIV | GGNNCC | 1 cut(s) | 805 |
| NmeAIII | GCCGAG | 1 cut(s) | 1716 |
| NmuCI | GTSAC | 4 cut(s) | 490, 511, 858, 985 |
| NsbI | TGCGCA | 1 cut(s) | 1607 |
| NsiI | ATGCAT | 1 cut(s) | 329 |
| NspI | RCATGY | 2 cut(s) | 799, 1736 |
| NspV | TTCGAA | 2 cut(s) | 252, 757 |
| OliI | CACNNNNGTG | 2 cut(s) | 509, 1761 |
| PaeI | GCATGC | 1 cut(s) | 1736 |
| PaeR7I | CTCGAG | 1 cut(s) | 245 |
| PagI | TCATGA | 3 cut(s) | 375, 636, 1483 |
| PciI | ACATGT | 1 cut(s) | 795 |
| PciSI | GCTCTTC | 1 cut(s) | 557 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 258, 734 |
| PctI | GAATGC | 2 cut(s) | 309, 1317 |
| PdmI | GAANNNNTTC | 1 cut(s) | 592 |
| PfeI | GAWTC | 5 cut(s) | 85, 824, 836, 1137, 1252 |
| PkrI | GCNGC | 1 cut(s) | 1047 |
| PleI | GAGTC | 3 cut(s) | 508, 865, 906 |
| PpsI | GAGTC | 3 cut(s) | 508, 865, 906 |
| Ppu21I | YACGTR | 2 cut(s) | 64, 316 |
| PscI | ACATGT | 1 cut(s) | 795 |
| PshBI | ATTAAT | 1 cut(s) | 1001 |
| Psp6I | CCWGG | 2 cut(s) | 1335, 1425 |
| PspGI | CCWGG | 2 cut(s) | 1335, 1425 |
| PspN4I | GGNNCC | 1 cut(s) | 805 |
| PspPI | GGNCC | 2 cut(s) | 241, 398 |
| PspXI | VCTCGAGB | 1 cut(s) | 245 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1614 |
| PsuI | RGATCY | 4 cut(s) | 127, 189, 681, 1644 |
| RsaI | GTAC | 7 cut(s) | 62, 124, 731, 805, 965, 1103, 1436 |
| RsaNI | GTAC | 7 cut(s) | 61, 123, 730, 804, 964, 1102, 1435 |
| RseI | CAYNNNNRTG | 2 cut(s) | 509, 1761 |
| SalI | GTCGAC | 1 cut(s) | 260 |
| SapI | GCTCTTC | 1 cut(s) | 557 |
| SaqAI | TTAA | 7 cut(s) | 57, 705, 887, 1001, 1203, 1311, 1457 |
| SatI | GCNGC | 1 cut(s) | 1046 |
| Sau3AI | GATC | 9 cut(s) | 127, 189, 283, 288, 567, 582, 681, 1266, 1644 |
| Sau96I | GGNCC | 2 cut(s) | 241, 398 |
| ScaI | AGTACT | 2 cut(s) | 1103, 1436 |
| SchI | GAGTC | 3 cut(s) | 508, 866, 906 |
| ScrFI | CCNGG | 3 cut(s) | 15, 1337, 1427 |
| SduI | GDGCHC | 5 cut(s) | 436, 534, 1221, 1555, 1758 |
| SfaNI | GCATC | 5 cut(s) | 159, 314, 336, 751, 1138 |
| Sfr274I | CTCGAG | 1 cut(s) | 245 |
| SfuI | TTCGAA | 2 cut(s) | 252, 757 |
| SinI | GGWCC | 1 cut(s) | 398 |
| SlaI | CTCGAG | 1 cut(s) | 245 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 509, 1761 |
| SmlI | CTYRAG | 2 cut(s) | 245, 704 |
| SmoI | CTYRAG | 2 cut(s) | 245, 704 |
| SnaBI | TACGTA | 1 cut(s) | 316 |
| SphI | GCATGC | 1 cut(s) | 1736 |
| Sse9I | AATT | 7 cut(s) | 18, 351, 658, 752, 763, 1088, 1586 |
| SsiI | CCGC | 1 cut(s) | 527 |
| SspI | AATATT | 1 cut(s) | 1569 |
| SspMI | CTAG | 1 cut(s) | 819 |
| StyD4I | CCNGG | 3 cut(s) | 13, 1335, 1425 |
| StyI | CCWWGG | 4 cut(s) | 35, 77, 807, 933 |
| TaaI | ACNGT | 2 cut(s) | 1064, 1545 |
| TaiI | ACGT | 2 cut(s) | 66, 318 |
| TaqI | TCGA | 8 cut(s) | 246, 252, 261, 405, 630, 663, 757, 1810 |
| TasI | AATT | 7 cut(s) | 18, 351, 658, 752, 763, 1088, 1586 |
| TatI | WGTACW | 2 cut(s) | 1101, 1434 |
| TfiI | GAWTC | 5 cut(s) | 85, 824, 836, 1137, 1252 |
| Tru1I | TTAA | 7 cut(s) | 57, 705, 887, 1001, 1203, 1311, 1457 |
| Tru9I | TTAA | 7 cut(s) | 57, 705, 887, 1001, 1203, 1311, 1457 |
| TscAI | CASTG | 4 cut(s) | 499, 659, 924, 990 |
| TseFI | GTSAC | 4 cut(s) | 490, 511, 858, 985 |
| TseI | GCWGC | 1 cut(s) | 1045 |
| Tsp45I | GTSAC | 4 cut(s) | 490, 511, 858, 985 |
| TspGWI | ACGGA | 1 cut(s) | 140 |
| TspRI | CASTG | 4 cut(s) | 499, 659, 924, 990 |
| Vha464I | CTTAAG | 1 cut(s) | 704 |
| VneI | GTGCAC | 1 cut(s) | 1551 |
| VpaK11BI | GGWCC | 1 cut(s) | 398 |
| VspI | ATTAAT | 1 cut(s) | 1001 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1361 |
| XapI | RAATTY | 3 cut(s) | 18, 351, 1088 |
| XceI | RCATGY | 2 cut(s) | 799, 1736 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 159 |
| XhoI | CTCGAG | 1 cut(s) | 245 |
| XmiI | GTMKAC | 4 cut(s) | 261, 719, 1066, 1668 |
| XmnI | GAANNNNTTC | 1 cut(s) | 592 |
| XspI | CTAG | 1 cut(s) | 819 |
| ZrmI | AGTACT | 2 cut(s) | 1103, 1436 |
| Zsp2I | ATGCAT | 1 cut(s) | 329 |