Prupe.2G113500_v2.0.a1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000300
Physical Location & Seq
Reverse (-)
17168568 .. 17172597
4030 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G113500.1

Sequence Viewer

Length: 1926 bp
ATGTATATATCATATACTCCTTCAAAGCTACACTACAAAGAAACAAAGAAGAATATGGCTTCTGTGCCTGGAATTCTTACTGAGTGGCCATGGAAAGCTCTTGGAAGCCTCAAGTACGTGATCTTGGCTCCATGGATCATCCACAGCACCTGGTTGTTTGTGGCTAACGATGCAAAAGAAAGAGACGTTTCATACTTCCTCTTGCTTGGAGTGGTGTTGTGGAGGATCATCCACAACCAGATATGGATCTCTCTGTCTCGATATCGAACTGCCAAAGGCAATGGCCGAATCCTTGACAGGGGTCTCGAATTCGAACAGGTCGACAGAGAAAACAATTGGGATGACCAGATATTGTTCAATGCACTCCTATTCTACACAGGCAGCAGGTACCTGCCTGGGGCTCAAAAGCTGCCACTTTGGAGGGCACATGGAGTTCTTTTAACCATTGTGCTTCATGCTGGTCCTGTGGAGTTCCTGTATTATTGGTTTCACAGAGCACTTCACCATCATTACCTTTACTCTCGCTACCATTCTCATCACCATTCCTCAATAGTTACTCAGCCAATTACTTCTGTGATTCATCCATTTGCGGAGCATGTAGTATATTCTGCGCTCTTCTTTATACCAATTTTGGGAACTATGTTGACGAGAACACTGTCTGTGGTATCTTTTACTGCTTATATTACTTACATTGACTTCATGAACAACATGGGGCACTGCAATTTCGAGCTCATTCCAAACTGGCTCTTTTCTCTATTTCCTCCTCTTAAGTATTTTATGTATACACCCTCGTACCACTCCTTGCATCACACACAATTCAGAACCAATTACTCACTTTTTATGCCAATTTACGATTACATATATGAAACCATAGACAAATCTTCGGATACTCTCTATAAAACTTCACTCAAGAGAGAGGAAGAAACGCCAGATGTCCTTCATCTAACCCATCTGACAACACCTGAGTCCATCTATCATCTACCGCTAGGATTTGCTTCCTTGGCCTCCCAGCCCCACACATCAAAATGGTACCTATGGCTGATGTGGCCTGTGACATTGTGGTCTATGATTCTAACTTGGATCTATGGCCGTACATTTGTGGTTGAGAGGCAACGTTTTGATAATCTCATATTACAAACTTGGGCTATACCAAAGTACAATTTGCAATACTACTTGCAATGGCAAAATGAAGCCATCAACAGTTTGATTGAGGAAGCCATAATTCAAGCAGAGGAAAAGGGTGTCAAAGTTTTATGTCTGGGTCTCTTAAATCAGGGTGAGGAGCTGAATAGATATGGTGGTGTGTACGTTCACAGGCATCCTCATCTCAAAATCAGGATTGTGGATGGAAGTAGCTTAGCTGTGGCCATAACCCTAAACACCATTCCAAAAGGAACCACCCAAGTTCTTCTTAGAGGCAACCTCACAAAGGTTGCTCATGCCGTTGCTTTTGCTTTGTGCCAGAAGGGTATCCAGATAACTACATTACACCACGATGAGTATTTGAAGCTCACCAAATCATTAAGTGGCATGGAAAGTAGCTTGGTTCTTGCAAAAAGTTATGCACATAAGATTTGGTTAGTGGGAGATGGATTGAGTGAAGAAGAACAATTGAGAGCACCAAAAGGAACATTATTTGTTCCTTTCTCCCAATTCCCACCAAAAAAATTGCGTAAAGACTGCTTCTACCACTACACTCCAGCAATGAAGACACCCCCATCTCTTGAGAATGTTCATGCTTGTGAGAATTGGTTGCCAAGAAGGGTGATGAGTGCATGGCGCATAGCAGGCATAGTGCATGCCTTGGAAGGCTGGAAGGAGCATGAGTGTGGGTACAACATGTCCAATATTGACAAAGTTTGGCAAGCAACTCTCCAACATGGCTTTCAGCCTCTCATCATCTCTACCACTCACACCAAAAATTAG

Protein Analysis

642

Amino Acids

74.59

Weight (kDa)

8.98

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1060
Acc36I ACCTGC 2 cut(s) 375, 399
Acc65I GGTACC 2 cut(s) 387, 1029
AccB1I GGYRCC 2 cut(s) 387, 1029
AccI GTMKAC 2 cut(s) 321, 782
AciI CCGC 2 cut(s) 590, 983
AclI AACGTT 1 cut(s) 1114
AclWI GGATC 4 cut(s) 143, 233, 254, 1088
AcoI YGGCCR 4 cut(s) 86, 283, 1087, 1365
AcsI RAATTY 2 cut(s) 72, 308
AfaI GTAC 8 cut(s) 116, 389, 794, 1031, 1093, 1157, 1307, 1835
AfiI CCNNNNNNNGG 4 cut(s) 298, 397, 632, 1429
AflII CTTAAG 1 cut(s) 767
AflIII ACRYGT 1 cut(s) 1839
AgsI TTSAA 4 cut(s) 24, 358, 1226, 1507
AjnI CCWGG 3 cut(s) 67, 149, 394
AjuI GAANNNNNNNTTGG 2 cut(s) 1869, 1901
AluBI AGCT 9 cut(s) 28, 98, 409, 730, 1285, 1356, 1361, 1510, 1542
AluI AGCT 9 cut(s) 28, 98, 409, 730, 1285, 1356, 1361, 1510, 1542
Alw21I GWGCWC 3 cut(s) 499, 732, 1621
Alw26I GTCTC 4 cut(s) 177, 261, 308, 1268
AlwI GGATC 4 cut(s) 143, 233, 254, 1088
AlwNI CAGNNNCTG 1 cut(s) 150
AoxI GGCC 6 cut(s) 86, 283, 1002, 1046, 1087, 1365
ApeKI GCWGC 2 cut(s) 381, 409
ApoI RAATTY 2 cut(s) 72, 308
Asp718I GGTACC 2 cut(s) 387, 1029
AspLEI GCGC 2 cut(s) 613, 1782
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 5 cut(s) 494, 530, 1289, 1504, 1777
AsuII TTCGAA 1 cut(s) 312
AvaII GGWCC 1 cut(s) 461
BaeGI GKGCMC 2 cut(s) 427, 717
BaeI ACNNNNGTAYC 2 cut(s) 776, 809
BalI TGGCCA 2 cut(s) 88, 1367
BanI GGYRCC 2 cut(s) 387, 1029
BanII GRGCYC 2 cut(s) 403, 732
BbsI GAAGAC 1 cut(s) 1715
Bbv12I GWGCWC 3 cut(s) 499, 732, 1621
BbvI GCAGC 2 cut(s) 393, 396
BccI CCATC 7 cut(s) 513, 957, 977, 1202, 1340, 1583, 1726
BceAI ACGGC 2 cut(s) 1074, 1427
BciT130I CCWGG 3 cut(s) 69, 151, 396
BciVI GTATCC 2 cut(s) 880, 1481
BcoDI GTCTC 4 cut(s) 177, 261, 308, 1268
BfaI CTAG 1 cut(s) 986
BfrI CTTAAG 1 cut(s) 767
BfuAI ACCTGC 2 cut(s) 375, 399
BfuI GTATCC 2 cut(s) 880, 1481
BisI GCNGC 2 cut(s) 382, 410
BlpI GCTNAGC 1 cut(s) 1357
BlsI GCNGC 2 cut(s) 383, 411
Bme1390I CCNGG 3 cut(s) 69, 151, 396
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 4 cut(s) 129, 389, 1031, 1396
BmrFI CCNGG 3 cut(s) 69, 151, 396
BmsI GCATC 3 cut(s) 160, 814, 1327
BoxI GACNNNNGTC 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 1715
BplI GAGNNNNNCTC 2 cut(s) 1407, 1439
BpmI CTGGAG 1 cut(s) 1683
Bpu1102I GCTNAGC 1 cut(s) 1357
Bpu14I TTCGAA 1 cut(s) 312
BpuEI CTTGAG 3 cut(s) 95, 893, 1745
BsaAI YACGTR 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 245
BsaI GGTCTC 2 cut(s) 308, 1268
BsaJI CCNNGG 5 cut(s) 89, 131, 395, 999, 1803
BsaXI ACNNNNNCTCC 4 cut(s) 461, 491, 1811, 1841
Bsc4I CCNNNNNNNGG 4 cut(s) 298, 397, 632, 1429
Bse1I ACTGG 1 cut(s) 746
Bse3DI GCAATG 3 cut(s) 286, 1184, 1710
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 3 cut(s) 69, 151, 396
BseDI CCNNGG 5 cut(s) 89, 131, 395, 999, 1803
BseGI GGATG 6 cut(s) 138, 228, 346, 580, 1318, 1351
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 4 cut(s) 298, 397, 632, 1429
BseMI GCAATG 3 cut(s) 286, 1184, 1710
BseMII CTCAG 3 cut(s) 72, 572, 954
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 2 cut(s) 753, 1295
BseSI GKGCMC 2 cut(s) 427, 717
BseXI GCAGC 2 cut(s) 393, 396
BseYI CCCAGC 1 cut(s) 1008
BshFI GGCC 6 cut(s) 88, 285, 1004, 1048, 1089, 1367
BshNI GGYRCC 2 cut(s) 387, 1029
BsiHKAI GWGCWC 3 cut(s) 499, 732, 1621
BslI CCNNNNNNNGG 4 cut(s) 298, 397, 632, 1429
BsmAI GTCTC 4 cut(s) 177, 261, 308, 1268
BsmBI CGTCTC 1 cut(s) 177
BsnI GGCC 6 cut(s) 88, 285, 1004, 1048, 1089, 1367
Bso31I GGTCTC 2 cut(s) 308, 1268
Bsp119I TTCGAA 1 cut(s) 312
Bsp1286I GDGCHC 6 cut(s) 403, 427, 499, 717, 732, 1621
Bsp143I GATC 5 cut(s) 120, 135, 225, 246, 1080
Bsp1720I GCTNAGC 1 cut(s) 1357
Bsp19I CCATGG 2 cut(s) 89, 131
BspACI CCGC 2 cut(s) 590, 983
BspANI GGCC 6 cut(s) 88, 285, 1004, 1048, 1089, 1367
BspCNI CTCAG 3 cut(s) 73, 571, 955
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 4 cut(s) 129, 389, 1031, 1396
BspMI ACCTGC 2 cut(s) 375, 399
BspPI GGATC 4 cut(s) 143, 233, 254, 1088
BspQI GCTCTTC 1 cut(s) 620
BspT104I TTCGAA 1 cut(s) 312
BspT107I GGYRCC 2 cut(s) 387, 1029
BspTI CTTAAG 1 cut(s) 767
BspTNI GGTCTC 2 cut(s) 308, 1268
BsrDI GCAATG 3 cut(s) 286, 1184, 1710
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 5 cut(s) 89, 131, 395, 999, 1803
BssMI GATC 5 cut(s) 120, 135, 225, 246, 1080
BssNAI GTATAC 1 cut(s) 783
BssT1I CCWWGG 4 cut(s) 89, 131, 999, 1803
Bst1107I GTATAC 1 cut(s) 783
Bst2UI CCWGG 3 cut(s) 69, 151, 396
Bst4CI ACNGT 2 cut(s) 657, 1202
Bst6I CTCTTC 1 cut(s) 620
BstAFI CTTAAG 1 cut(s) 767
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 312
BstC8I GCNNGC 3 cut(s) 1789, 1800, 1866
BstDEI CTNAG 5 cut(s) 81, 558, 963, 1357, 1412
BstDSI CCRYGG 2 cut(s) 89, 131
BstENI CCTNNNNNAGG 1 cut(s) 1427
BstF5I GGATG 6 cut(s) 138, 228, 346, 580, 1318, 1351
BstHHI GCGC 2 cut(s) 613, 1782
BstKTI GATC 5 cut(s) 123, 138, 228, 249, 1083
BstMAI GTCTC 4 cut(s) 177, 261, 308, 1268
BstMBI GATC 5 cut(s) 120, 135, 225, 246, 1080
BstMWI GCNNNNNNNGC 4 cut(s) 170, 1001, 1045, 1788
BstNI CCWGG 3 cut(s) 69, 151, 396
BstNSI RCATGY 3 cut(s) 599, 1802, 1843
BstPAI GACNNNNGTC 1 cut(s) 300
BstSCI CCNGG 3 cut(s) 67, 149, 394
BstSLI GKGCMC 2 cut(s) 427, 717
BstV1I GCAGC 2 cut(s) 393, 396
BstV2I GAAGAC 1 cut(s) 1715
BstX2I RGATCY 2 cut(s) 246, 1080
BstYI RGATCY 2 cut(s) 246, 1080
BstZ17I GTATAC 1 cut(s) 783
BsuI GTATCC 2 cut(s) 880, 1481
BsuRI GGCC 6 cut(s) 88, 285, 1004, 1048, 1089, 1367
BtgI CCRYGG 2 cut(s) 89, 131
BtsCI GGATG 6 cut(s) 138, 228, 346, 580, 1318, 1351
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 2 cut(s) 653, 715
BveI ACCTGC 2 cut(s) 375, 399
Cac8I GCNNGC 3 cut(s) 1789, 1800, 1866
CaiI CAGNNNCTG 1 cut(s) 150
CciI TCATGA 1 cut(s) 699
CfoI GCGC 2 cut(s) 613, 1782
Cfr13I GGNCC 1 cut(s) 461
CsiI ACCWGGT 1 cut(s) 149
Csp6I GTAC 8 cut(s) 115, 388, 793, 1030, 1092, 1156, 1306, 1834
CviQI GTAC 8 cut(s) 115, 388, 793, 1030, 1092, 1156, 1306, 1834
DdeI CTNAG 5 cut(s) 81, 558, 963, 1357, 1412
DpnI GATC 5 cut(s) 122, 137, 227, 248, 1082
DpnII GATC 5 cut(s) 120, 135, 225, 246, 1080
DrdI GACNNNNNNGTC 1 cut(s) 1060
DseDI GACNNNNNNGTC 1 cut(s) 1060
EaeI YGGCCR 4 cut(s) 86, 283, 1087, 1365
Eam1104I CTCTTC 1 cut(s) 620
EarI CTCTTC 1 cut(s) 620
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 4 cut(s) 89, 131, 999, 1803
Eco24I GRGCYC 2 cut(s) 403, 732
Eco31I GGTCTC 2 cut(s) 308, 1268
Eco32I GATATC 1 cut(s) 263
Eco47I GGWCC 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 730
EcoNI CCTNNNNNAGG 1 cut(s) 1427
EcoRI GAATTC 2 cut(s) 72, 308
EcoRII CCWGG 3 cut(s) 67, 149, 394
EcoRV GATATC 1 cut(s) 263
EcoT14I CCWWGG 4 cut(s) 89, 131, 999, 1803
EcoT38I GRGCYC 2 cut(s) 403, 732
ErhI CCWWGG 4 cut(s) 89, 131, 999, 1803
Esp3I CGTCTC 1 cut(s) 177
FalI AAGNNNNNCTT 2 cut(s) 1395, 1427
FblI GTMKAC 2 cut(s) 321, 782
Fnu4HI GCNGC 2 cut(s) 382, 410
FokI GGATG 6 cut(s) 125, 215, 353, 567, 1305, 1358
FriOI GRGCYC 2 cut(s) 403, 732
Fsp4HI GCNGC 2 cut(s) 382, 410
FspBI CTAG 1 cut(s) 986
GlaI GCGC 2 cut(s) 612, 1781
GluI GCNGC 2 cut(s) 382, 410
GsaI CCCAGC 1 cut(s) 1012
GsuI CTGGAG 1 cut(s) 1683
HaeIII GGCC 6 cut(s) 88, 285, 1004, 1048, 1089, 1367
HhaI GCGC 2 cut(s) 613, 1782
Hin6I GCGC 2 cut(s) 611, 1780
HinP1I GCGC 2 cut(s) 611, 1780
HincII GTYRAC 2 cut(s) 322, 645
HindII GTYRAC 2 cut(s) 322, 645
HinfI GANTC 4 cut(s) 288, 577, 965, 1069
HphI GGTGA 5 cut(s) 494, 530, 1289, 1504, 1777
Hpy166II GTNNAC 5 cut(s) 322, 645, 783, 1306, 1312
Hpy188I TCNGA 3 cut(s) 821, 886, 954
Hpy188III TCNNGA 7 cut(s) 258, 305, 700, 910, 1336, 1474, 1724
Hpy8I GTNNAC 5 cut(s) 322, 645, 783, 1306, 1312
HpyAV CCTTC 6 cut(s) 30, 947, 1459, 1755, 1802, 1810
HpyCH4III ACNGT 2 cut(s) 657, 1202
HpyCH4IV ACGT 4 cut(s) 117, 186, 1114, 1308
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 1001, 1045, 1788
HpyF3I CTNAG 5 cut(s) 81, 558, 963, 1357, 1412
HpySE526I ACGT 4 cut(s) 117, 186, 1114, 1308
HspAI GCGC 2 cut(s) 611, 1780
KpnI GGTACC 2 cut(s) 391, 1033
Kzo9I GATC 5 cut(s) 120, 135, 225, 246, 1080
LguI GCTCTTC 1 cut(s) 620
LmnI GCTCC 4 cut(s) 133, 592, 1282, 1819
Lsp1109I GCAGC 2 cut(s) 393, 396
LweI GCATC 3 cut(s) 160, 814, 1327
MabI ACCWGGT 1 cut(s) 149
MaeI CTAG 1 cut(s) 986
MaeII ACGT 4 cut(s) 117, 186, 1114, 1308
MaeIII GTNAC 2 cut(s) 553, 1051
MalI GATC 5 cut(s) 122, 137, 227, 248, 1082
MboI GATC 5 cut(s) 120, 135, 225, 246, 1080
MboII GAAGA 8 cut(s) 61, 607, 873, 932, 1400, 1613, 1616, 1720
MfeI CAATTG 2 cut(s) 334, 1610
MflI RGATCY 2 cut(s) 246, 1080
MhlI GDGCHC 6 cut(s) 403, 427, 499, 717, 732, 1621
MlsI TGGCCA 2 cut(s) 88, 1367
MluNI TGGCCA 2 cut(s) 88, 1367
MlyI GAGTC 1 cut(s) 974
MmeI TCCRAC 1 cut(s) 1900
Mox20I TGGCCA 2 cut(s) 88, 1367
MscI TGGCCA 2 cut(s) 88, 1367
MseI TTAA 4 cut(s) 440, 768, 1268, 1523
MslI CAYNNNNRTG 4 cut(s) 1024, 1494, 1740, 1827
Msp20I TGGCCA 2 cut(s) 88, 1367
MspCI CTTAAG 1 cut(s) 767
MspR9I CCNGG 3 cut(s) 69, 151, 396
MunI CAATTG 2 cut(s) 334, 1610
MvaI CCWGG 3 cut(s) 69, 151, 396
MwoI GCNNNNNNNGC 4 cut(s) 170, 1001, 1045, 1788
NcoI CCATGG 2 cut(s) 89, 131
NdeII GATC 5 cut(s) 120, 135, 225, 246, 1080
NlaIV GGNNCC 4 cut(s) 129, 389, 1031, 1396
NmuCI GTSAC 1 cut(s) 1051
NspI RCATGY 3 cut(s) 599, 1802, 1843
NspV TTCGAA 1 cut(s) 312
PaeI GCATGC 1 cut(s) 1802
PagI TCATGA 1 cut(s) 699
PciI ACATGT 1 cut(s) 1839
PciSI GCTCTTC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PfeI GAWTC 3 cut(s) 288, 577, 1069
PkrI GCNGC 2 cut(s) 383, 411
PleI GAGTC 1 cut(s) 973
PpsI GAGTC 1 cut(s) 973
Ppu21I YACGTR 1 cut(s) 118
PscI ACATGT 1 cut(s) 1839
PshAI GACNNNNGTC 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 732
Psp1406I AACGTT 1 cut(s) 1114
Psp6I CCWGG 3 cut(s) 67, 149, 394
PspFI CCCAGC 1 cut(s) 1008
PspGI CCWGG 3 cut(s) 67, 149, 394
PspN4I GGNNCC 4 cut(s) 129, 389, 1031, 1396
PspPI GGNCC 1 cut(s) 461
PstNI CAGNNNCTG 1 cut(s) 150
PsuI RGATCY 2 cut(s) 246, 1080
RsaI GTAC 8 cut(s) 116, 389, 794, 1031, 1093, 1157, 1307, 1835
RsaNI GTAC 8 cut(s) 115, 388, 793, 1030, 1092, 1156, 1306, 1834
RseI CAYNNNNRTG 4 cut(s) 1024, 1494, 1740, 1827
SacI GAGCTC 1 cut(s) 732
SalI GTCGAC 1 cut(s) 320
SapI GCTCTTC 1 cut(s) 620
SaqAI TTAA 4 cut(s) 440, 768, 1268, 1523
SatI GCNGC 2 cut(s) 382, 410
Sau3AI GATC 5 cut(s) 120, 135, 225, 246, 1080
Sau96I GGNCC 1 cut(s) 461
SchI GAGTC 1 cut(s) 974
ScrFI CCNGG 3 cut(s) 69, 151, 396
SduI GDGCHC 6 cut(s) 403, 427, 499, 717, 732, 1621
SexAI ACCWGGT 1 cut(s) 149
SfaNI GCATC 3 cut(s) 160, 814, 1327
SfuI TTCGAA 1 cut(s) 312
SinI GGWCC 1 cut(s) 461
SmiMI CAYNNNNRTG 4 cut(s) 1024, 1494, 1740, 1827
SmlI CTYRAG 4 cut(s) 110, 767, 908, 1724
SmoI CTYRAG 4 cut(s) 110, 767, 908, 1724
SphI GCATGC 1 cut(s) 1802
SsiI CCGC 2 cut(s) 590, 983
SspI AATATT 1 cut(s) 1849
SspMI CTAG 1 cut(s) 986
SstI GAGCTC 1 cut(s) 732
StyD4I CCNGG 3 cut(s) 67, 149, 394
StyI CCWWGG 4 cut(s) 89, 131, 999, 1803
TaaI ACNGT 2 cut(s) 657, 1202
TaiI ACGT 4 cut(s) 120, 189, 1117, 1311
TaqI TCGA 6 cut(s) 259, 265, 306, 312, 321, 726
TatI WGTACW 1 cut(s) 1155
TfiI GAWTC 3 cut(s) 288, 577, 1069
Tru1I TTAA 4 cut(s) 440, 768, 1268, 1523
Tru9I TTAA 4 cut(s) 440, 768, 1268, 1523
TscAI CASTG 2 cut(s) 660, 722
TseFI GTSAC 1 cut(s) 1051
TseI GCWGC 2 cut(s) 381, 409
Tsp45I GTSAC 1 cut(s) 1051
TspRI CASTG 2 cut(s) 660, 722
Vha464I CTTAAG 1 cut(s) 767
VpaK11BI GGWCC 1 cut(s) 461
XagI CCTNNNNNAGG 1 cut(s) 1427
XapI RAATTY 2 cut(s) 72, 308
XceI RCATGY 3 cut(s) 599, 1802, 1843
XmiI GTMKAC 2 cut(s) 321, 782
XspI CTAG 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.