Prupe.2G113600_v2.0.a1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000300
Physical Location & Seq
Reverse (-)
17180945 .. 17185128
4184 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G113600.1

Sequence Viewer

Length: 1890 bp
ATGGCTTCTGTGCCTGGAATTCTTACTGATTGGCCATGGACAGCTCTTGGAAGCTTCAAGCACGTGATCTTGGCTCCATGGGTCATCCACAGCACCTGGTTGTTGGTTGCTAACGATGGAAAAGAAAGAGACATTTCATACTTCCTCATACTTGCAGTTGTGTTGTGGAGGATCCTCCACAACCAGATATGGATCTCTCTGTCTCGATACCGAACTGCCAAAGGCAATGGCCGGATCCTTGACAAGGGTCTCGAATTCGAACAGGTCGACAGAGAAAACAATTGGGATGACCAAATATTGTTCAATGCACTCCTGTTCTACACAGGGAGCAGGTACCTTCCTGGTGGTCAACACGCACCGTTTTGGAGGGCAGAGGGACTTCTTTTGACCATTGTGCTTCATGCTGGTCCTGTGGAGTTCCTGTATTATTGGTTTCACAGAGCACTTCACCATCATTACCTTTACTCTCGCTACCATTCTCATCACCATTCCTCAATCGTTACTCAGCCAATTACTTCTGTGATTCATCCATTTGCGGAGCATATAGTATATTCTACGCTCTTCTTTATACCAATTTTGGGAACTATGTTGACGAGAACTACGTCTGTGGTATCTTTTACTGCTTATATTACTTACATTGACTTCATGAACAACATGGGGCACTGCAATTTCGAGCTCATTCCAAACTGGCTCTTTTCTCTATTTCCTCCTCTCAAGTATCTTATGTATACACCCTCGTACCACTCCTTGCATCACACACAATTTCGAACCAATTACTCACTTTTCATGCCAATCTACGACTACATATATAAAACCATGGACAAATCTTCGGATGTTCTCTACGAAACTTCACTCAAGAGAGAGGAAGAAACACCAGATATCCTTCATCTAACCCATCTAACAACACCTGAGTCCATTTATCATCTACCGCTAGGATTTGCTTCCCTAGCCTCTAGGCCCTACACATCAAACTGGTACCTGTGGTTGATGTGGCCCGTGACATTCTGGTCTATGATTTTAACTTGGATCTATGGCCGTACATTTGTGGTTGACAGGCACCGTTTTGATAATCTCAAATTACAAACTTGGGCTATACCAAAGTACAGTTTGCAATACTACTTGCAATGGCAAAATGAAGCTATCAACGGTCTGATTGAGGAAGCCATAATTCAAGCAGAGGAAAAGGGTGTCAAAGTCTTATGTCTTGGTCTCTTAAATCAGGGTGAGGAGCTGAATAGATATGGTGGTCTGTATGTTCACAGGCATCCTAATCTCAAAATAAGGGTTGTGGATGGAAGTAGCTTAGCTGTGGCCATAACCCTAAACACCATTCCAAAAGGGACCACCCAAGTTCTTCTTAGAGGCAACCTCACAAAGGTTGCTTATGCCATTGCTTTTGCTTTGTGCCAGAAGGGTATCCAGGTAGTTACATTACACAAGGATGAGTATTTGAAGCTCACCAAATCATTCAGTAGCACTGAAAGTAGCTTGGTTCTTGCAAAAAGCTATGCACATAAGATTTGGTTAGTGGGTGATGGATTGAGTGCAGAAGAACAACTGAGAGCACCAAAAGGAACATTATTTGTTCCCTTCTCCCAATTGCCACCAAAAAAACTGCGCAAAGATTGTTTTTACCACTACCCACCAGCAATGAAGACTCCCACATCTCTTGAGAATATTCATGCTTGTGAGAATTGGTTGCCAAGAAGGGTGATGAGTGCATGGCGCATAGCTGGCATAGTGCATGCCTTGGAAGGTTGGAAGGAGCATGAGTGTGGCTACACAATGTCCAATGTTGACAAAGTTTGGCAAGCAACTCTTCAACATGGCTTTCAGCCTCTCATCATCACACCAAAATTAGTCGATCGCCTTCATCAAAACATTAATTAA

Protein Analysis

630

Amino Acids

73.01

Weight (kDa)

8.79

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1006
Acc16I TGCGCA 1 cut(s) 1619
Acc36I ACCTGC 1 cut(s) 321
Acc65I GGTACC 2 cut(s) 333, 975
AccB1I GGYRCC 3 cut(s) 333, 975, 1056
AccI GTMKAC 2 cut(s) 267, 728
AciI CCGC 2 cut(s) 536, 929
AclWI GGATC 6 cut(s) 166, 179, 200, 229, 242, 1034
AcoI YGGCCR 4 cut(s) 32, 229, 1033, 1311
AcsI RAATTY 2 cut(s) 18, 254
AcvI CACGTG 1 cut(s) 64
AfaI GTAC 5 cut(s) 335, 740, 977, 1039, 1103
AfiI CCNNNNNNNGG 3 cut(s) 244, 578, 1375
AgsI TTSAA 5 cut(s) 58, 304, 1172, 1453, 1823
AjnI CCWGG 4 cut(s) 13, 95, 340, 1419
Alw21I GWGCWC 3 cut(s) 445, 678, 1567
Alw26I GTCTC 4 cut(s) 123, 207, 254, 1214
AlwI GGATC 6 cut(s) 166, 179, 200, 229, 242, 1034
AlwNI CAGNNNCTG 1 cut(s) 96
AoxI GGCC 6 cut(s) 32, 229, 956, 992, 1033, 1311
ApoI RAATTY 2 cut(s) 18, 254
AseI ATTAAT 1 cut(s) 1884
Asp718I GGTACC 2 cut(s) 333, 975
AspLEI GCGC 2 cut(s) 1620, 1728
AspS9I GGNCC 4 cut(s) 407, 957, 993, 1341
AsuHPI GGTGA 6 cut(s) 440, 476, 1235, 1450, 1544, 1723
AsuII TTCGAA 2 cut(s) 258, 766
AvaII GGWCC 2 cut(s) 407, 1341
BaeGI GKGCMC 1 cut(s) 663
BaeI ACNNNNGTAYC 2 cut(s) 722, 755
BalI TGGCCA 2 cut(s) 34, 1313
BamHI GGATCC 2 cut(s) 171, 234
BanI GGYRCC 3 cut(s) 333, 975, 1056
BanII GRGCYC 1 cut(s) 678
BbrPI CACGTG 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 1661
Bbv12I GWGCWC 3 cut(s) 445, 678, 1567
BccI CCATC 5 cut(s) 110, 459, 903, 1286, 1529
BceAI ACGGC 1 cut(s) 1020
BciT130I CCWGG 4 cut(s) 15, 97, 342, 1421
BciVI GTATCC 1 cut(s) 1427
BcoDI GTCTC 4 cut(s) 123, 207, 254, 1214
BfaI CTAG 3 cut(s) 932, 947, 954
BfuAI ACCTGC 1 cut(s) 321
BfuI GTATCC 1 cut(s) 1427
BlpI GCTNAGC 1 cut(s) 1303
Bme1390I CCNGG 4 cut(s) 15, 97, 342, 1421
Bme18I GGWCC 2 cut(s) 407, 1341
BmgT120I GGNCC 4 cut(s) 407, 957, 993, 1341
BmiI GGNNCC 7 cut(s) 75, 173, 236, 335, 977, 1058, 1342
BmrFI CCNGG 4 cut(s) 15, 97, 342, 1421
BmsI GCATC 2 cut(s) 760, 1273
BoxI GACNNNNGTC 1 cut(s) 246
BpiI GAAGAC 1 cut(s) 1661
BplI GAGNNNNNCTC 2 cut(s) 1353, 1385
Bpu1102I GCTNAGC 1 cut(s) 1303
Bpu14I TTCGAA 2 cut(s) 258, 766
BpuEI CTTGAG 3 cut(s) 698, 839, 1691
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 191
BsaI GGTCTC 2 cut(s) 254, 1214
BsaJI CCNNGG 4 cut(s) 35, 77, 816, 1749
BsaXI ACNNNNNCTCC 4 cut(s) 407, 437, 1757, 1787
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 578, 1375
Bse1I ACTGG 2 cut(s) 692, 977
Bse3DI GCAATG 4 cut(s) 232, 1130, 1389, 1656
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 4 cut(s) 15, 97, 342, 1421
BseDI CCNNGG 4 cut(s) 35, 77, 816, 1749
BseGI GGATG 7 cut(s) 84, 292, 526, 838, 1264, 1297, 1447
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 3 cut(s) 244, 578, 1375
BseMI GCAATG 4 cut(s) 232, 1130, 1389, 1656
BseMII CTCAG 3 cut(s) 518, 900, 1550
BseNI ACTGG 2 cut(s) 692, 977
BseRI GAGGAG 2 cut(s) 699, 1241
BseSI GKGCMC 1 cut(s) 663
BsgI GTGCAG 1 cut(s) 1566
Bsh1285I CGRYCG 1 cut(s) 1867
BshFI GGCC 6 cut(s) 34, 231, 958, 994, 1035, 1313
BshNI GGYRCC 3 cut(s) 333, 975, 1056
BsiEI CGRYCG 1 cut(s) 1867
BsiHKAI GWGCWC 3 cut(s) 445, 678, 1567
BsiSI CCGG 1 cut(s) 232
BslFI GGGAC 2 cut(s) 390, 1354
BslI CCNNNNNNNGG 3 cut(s) 244, 578, 1375
BsmAI GTCTC 4 cut(s) 123, 207, 254, 1214
BsmFI GGGAC 2 cut(s) 390, 1354
BsnI GGCC 6 cut(s) 34, 231, 958, 994, 1035, 1313
Bso31I GGTCTC 2 cut(s) 254, 1214
Bsp119I TTCGAA 2 cut(s) 258, 766
Bsp1286I GDGCHC 4 cut(s) 445, 663, 678, 1567
Bsp143I GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
Bsp1720I GCTNAGC 1 cut(s) 1303
Bsp19I CCATGG 3 cut(s) 35, 77, 816
BspACI CCGC 2 cut(s) 536, 929
BspANI GGCC 6 cut(s) 34, 231, 958, 994, 1035, 1313
BspCNI CTCAG 3 cut(s) 517, 901, 1551
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 7 cut(s) 75, 173, 236, 335, 977, 1058, 1342
BspMI ACCTGC 1 cut(s) 321
BspPI GGATC 6 cut(s) 166, 179, 200, 229, 242, 1034
BspQI GCTCTTC 1 cut(s) 566
BspT104I TTCGAA 2 cut(s) 258, 766
BspT107I GGYRCC 3 cut(s) 333, 975, 1056
BspTNI GGTCTC 2 cut(s) 254, 1214
BsrDI GCAATG 4 cut(s) 232, 1130, 1389, 1656
BsrI ACTGG 2 cut(s) 692, 977
BssECI CCNNGG 4 cut(s) 35, 77, 816, 1749
BssMI GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
BssNAI GTATAC 1 cut(s) 729
BssT1I CCWWGG 4 cut(s) 35, 77, 816, 1749
Bst1107I GTATAC 1 cut(s) 729
Bst2UI CCWGG 4 cut(s) 15, 97, 342, 1421
Bst4CI ACNGT 4 cut(s) 360, 1061, 1106, 1148
Bst6I CTCTTC 2 cut(s) 566, 1824
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 2 cut(s) 258, 766
BstC8I GCNNGC 3 cut(s) 1735, 1746, 1812
BstDEI CTNAG 5 cut(s) 504, 909, 1303, 1358, 1559
BstDSI CCRYGG 3 cut(s) 35, 77, 816
BstENI CCTNNNNNAGG 2 cut(s) 242, 1373
BstF5I GGATG 7 cut(s) 84, 292, 526, 838, 1264, 1297, 1447
BstHHI GCGC 2 cut(s) 1620, 1728
BstKTI GATC 6 cut(s) 69, 174, 195, 237, 1029, 1867
BstMAI GTCTC 4 cut(s) 123, 207, 254, 1214
BstMBI GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
BstMCI CGRYCG 1 cut(s) 1867
BstMWI GCNNNNNNNGC 2 cut(s) 947, 1734
BstNI CCWGG 4 cut(s) 15, 97, 342, 1421
BstNSI RCATGY 1 cut(s) 1748
BstPAI GACNNNNGTC 1 cut(s) 246
BstSCI CCNGG 4 cut(s) 13, 95, 340, 1419
BstSLI GKGCMC 1 cut(s) 663
BstV2I GAAGAC 1 cut(s) 1661
BstX2I RGATCY 4 cut(s) 171, 192, 234, 1026
BstYI RGATCY 4 cut(s) 171, 192, 234, 1026
BstZ17I GTATAC 1 cut(s) 729
BsuI GTATCC 1 cut(s) 1427
BsuRI GGCC 6 cut(s) 34, 231, 958, 994, 1035, 1313
BtgI CCRYGG 3 cut(s) 35, 77, 816
BtsCI GGATG 7 cut(s) 84, 292, 526, 838, 1264, 1297, 1447
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 2 cut(s) 661, 1476
BveI ACCTGC 1 cut(s) 321
Cac8I GCNNGC 3 cut(s) 1735, 1746, 1812
CaiI CAGNNNCTG 1 cut(s) 96
CciI TCATGA 1 cut(s) 645
CfoI GCGC 2 cut(s) 1620, 1728
Cfr13I GGNCC 4 cut(s) 407, 957, 993, 1341
CsiI ACCWGGT 1 cut(s) 95
Csp6I GTAC 5 cut(s) 334, 739, 976, 1038, 1102
CspCI CAANNNNNGTGG 2 cut(s) 1651, 1686
CviQI GTAC 5 cut(s) 334, 739, 976, 1038, 1102
DdeI CTNAG 5 cut(s) 504, 909, 1303, 1358, 1559
DpnI GATC 6 cut(s) 68, 173, 194, 236, 1028, 1866
DpnII GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
DrdI GACNNNNNNGTC 1 cut(s) 1006
DseDI GACNNNNNNGTC 1 cut(s) 1006
EaeI YGGCCR 4 cut(s) 32, 229, 1033, 1311
Eam1104I CTCTTC 2 cut(s) 566, 1824
EarI CTCTTC 2 cut(s) 566, 1824
Ecl136II GAGCTC 1 cut(s) 676
Eco130I CCWWGG 4 cut(s) 35, 77, 816, 1749
Eco24I GRGCYC 1 cut(s) 678
Eco31I GGTCTC 2 cut(s) 254, 1214
Eco32I GATATC 1 cut(s) 880
Eco47I GGWCC 2 cut(s) 407, 1341
Eco53kI GAGCTC 1 cut(s) 676
Eco72I CACGTG 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 676
EcoNI CCTNNNNNAGG 2 cut(s) 242, 1373
EcoO109I RGGNCCY 1 cut(s) 957
EcoRI GAATTC 2 cut(s) 18, 254
EcoRII CCWGG 4 cut(s) 13, 95, 340, 1419
EcoRV GATATC 1 cut(s) 880
EcoT14I CCWWGG 4 cut(s) 35, 77, 816, 1749
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 4 cut(s) 35, 77, 816, 1749
FalI AAGNNNNNCTT 4 cut(s) 1341, 1373, 1803, 1835
FaqI GGGAC 2 cut(s) 390, 1354
FblI GTMKAC 2 cut(s) 267, 728
FokI GGATG 7 cut(s) 71, 299, 513, 845, 1251, 1304, 1454
FriOI GRGCYC 1 cut(s) 678
FspBI CTAG 3 cut(s) 932, 947, 954
FspI TGCGCA 1 cut(s) 1619
GlaI GCGC 2 cut(s) 1619, 1727
HaeIII GGCC 6 cut(s) 34, 231, 958, 994, 1035, 1313
HapII CCGG 1 cut(s) 232
HhaI GCGC 2 cut(s) 1620, 1728
Hin6I GCGC 2 cut(s) 1618, 1726
HinP1I GCGC 2 cut(s) 1618, 1726
HincII GTYRAC 5 cut(s) 268, 350, 591, 1051, 1798
HindII GTYRAC 5 cut(s) 268, 350, 591, 1051, 1798
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 3 cut(s) 523, 911, 1657
HpaII CCGG 1 cut(s) 232
HphI GGTGA 6 cut(s) 440, 476, 1235, 1450, 1544, 1723
Hpy166II GTNNAC 7 cut(s) 268, 350, 591, 729, 1051, 1258, 1798
Hpy188I TCNGA 2 cut(s) 832, 1152
Hpy188III TCNNGA 5 cut(s) 204, 251, 646, 856, 1670
Hpy8I GTNNAC 7 cut(s) 268, 350, 591, 729, 1051, 1258, 1798
HpyAV CCTTC 8 cut(s) 347, 893, 1405, 1600, 1701, 1748, 1756, 1880
HpyCH4III ACNGT 4 cut(s) 360, 1061, 1106, 1148
HpyCH4IV ACGT 2 cut(s) 63, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 947, 1734
HpyF3I CTNAG 5 cut(s) 504, 909, 1303, 1358, 1559
HpySE526I ACGT 2 cut(s) 63, 602
HspAI GCGC 2 cut(s) 1618, 1726
KpnI GGTACC 2 cut(s) 337, 979
Kzo9I GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
LguI GCTCTTC 1 cut(s) 566
LmnI GCTCC 5 cut(s) 79, 327, 538, 1228, 1765
LweI GCATC 2 cut(s) 760, 1273
MabI ACCWGGT 1 cut(s) 95
MaeI CTAG 3 cut(s) 932, 947, 954
MaeII ACGT 2 cut(s) 63, 602
MaeIII GTNAC 3 cut(s) 499, 997, 1426
MalI GATC 6 cut(s) 68, 173, 194, 236, 1028, 1866
MboI GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
MboII GAAGA 7 cut(s) 553, 819, 878, 1346, 1562, 1666, 1811
MfeI CAATTG 2 cut(s) 280, 1598
MflI RGATCY 4 cut(s) 171, 192, 234, 1026
MhlI GDGCHC 4 cut(s) 445, 663, 678, 1567
MlsI TGGCCA 2 cut(s) 34, 1313
MluNI TGGCCA 2 cut(s) 34, 1313
MlyI GAGTC 2 cut(s) 920, 1651
MmeI TCCRAC 1 cut(s) 1739
Mox20I TGGCCA 2 cut(s) 34, 1313
MscI TGGCCA 2 cut(s) 34, 1313
MseI TTAA 4 cut(s) 1019, 1214, 1884, 1888
MslI CAYNNNNRTG 3 cut(s) 1440, 1686, 1773
Msp20I TGGCCA 2 cut(s) 34, 1313
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 4 cut(s) 15, 97, 342, 1421
MunI CAATTG 2 cut(s) 280, 1598
MvaI CCWGG 4 cut(s) 15, 97, 342, 1421
MwoI GCNNNNNNNGC 2 cut(s) 947, 1734
NcoI CCATGG 3 cut(s) 35, 77, 816
NdeII GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
NlaIV GGNNCC 7 cut(s) 75, 173, 236, 335, 977, 1058, 1342
NmuCI GTSAC 1 cut(s) 997
NsbI TGCGCA 1 cut(s) 1619
NspI RCATGY 1 cut(s) 1748
NspV TTCGAA 2 cut(s) 258, 766
PacI TTAATTAA 1 cut(s) 1888
PaeI GCATGC 1 cut(s) 1748
PagI TCATGA 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 566
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 599
PfeI GAWTC 1 cut(s) 523
Ple19I CGATCG 1 cut(s) 1867
PleI GAGTC 2 cut(s) 919, 1651
PmaCI CACGTG 1 cut(s) 64
PmlI CACGTG 1 cut(s) 64
PpsI GAGTC 2 cut(s) 919, 1651
Ppu21I YACGTR 1 cut(s) 64
PshAI GACNNNNGTC 1 cut(s) 246
PshBI ATTAAT 1 cut(s) 1884
Psp124BI GAGCTC 1 cut(s) 678
Psp6I CCWGG 4 cut(s) 13, 95, 340, 1419
PspCI CACGTG 1 cut(s) 64
PspGI CCWGG 4 cut(s) 13, 95, 340, 1419
PspN4I GGNNCC 7 cut(s) 75, 173, 236, 335, 977, 1058, 1342
PspPI GGNCC 4 cut(s) 407, 957, 993, 1341
PstNI CAGNNNCTG 1 cut(s) 96
PsuI RGATCY 4 cut(s) 171, 192, 234, 1026
PvuI CGATCG 1 cut(s) 1867
RsaI GTAC 5 cut(s) 335, 740, 977, 1039, 1103
RsaNI GTAC 5 cut(s) 334, 739, 976, 1038, 1102
RseI CAYNNNNRTG 3 cut(s) 1440, 1686, 1773
SacI GAGCTC 1 cut(s) 678
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 566
SaqAI TTAA 4 cut(s) 1019, 1214, 1884, 1888
Sau3AI GATC 6 cut(s) 66, 171, 192, 234, 1026, 1864
Sau96I GGNCC 4 cut(s) 407, 957, 993, 1341
SchI GAGTC 2 cut(s) 920, 1651
ScrFI CCNGG 4 cut(s) 15, 97, 342, 1421
SduI GDGCHC 4 cut(s) 445, 663, 678, 1567
SexAI ACCWGGT 1 cut(s) 95
SfaNI GCATC 2 cut(s) 760, 1273
SfuI TTCGAA 2 cut(s) 258, 766
SinI GGWCC 2 cut(s) 407, 1341
SmiMI CAYNNNNRTG 3 cut(s) 1440, 1686, 1773
SmlI CTYRAG 3 cut(s) 713, 854, 1670
SmoI CTYRAG 3 cut(s) 713, 854, 1670
SphI GCATGC 1 cut(s) 1748
SsiI CCGC 2 cut(s) 536, 929
SspI AATATT 2 cut(s) 297, 1678
SspMI CTAG 3 cut(s) 932, 947, 954
SstI GAGCTC 1 cut(s) 678
StyD4I CCNGG 4 cut(s) 13, 95, 340, 1419
StyI CCWWGG 4 cut(s) 35, 77, 816, 1749
TaaI ACNGT 4 cut(s) 360, 1061, 1106, 1148
TaiI ACGT 2 cut(s) 66, 605
TaqI TCGA 7 cut(s) 205, 252, 258, 267, 672, 766, 1863
TatI WGTACW 1 cut(s) 1101
TfiI GAWTC 1 cut(s) 523
Tru1I TTAA 4 cut(s) 1019, 1214, 1884, 1888
Tru9I TTAA 4 cut(s) 1019, 1214, 1884, 1888
TscAI CASTG 2 cut(s) 668, 1483
TseFI GTSAC 1 cut(s) 997
Tsp45I GTSAC 1 cut(s) 997
TspRI CASTG 2 cut(s) 668, 1483
VpaK11BI GGWCC 2 cut(s) 407, 1341
VspI ATTAAT 1 cut(s) 1884
XagI CCTNNNNNAGG 2 cut(s) 242, 1373
XapI RAATTY 2 cut(s) 18, 254
XceI RCATGY 1 cut(s) 1748
XmiI GTMKAC 2 cut(s) 267, 728
XspI CTAG 3 cut(s) 932, 947, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.