Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000300

Physical Location & Seq

Genomic Coordinates Forward (+)

8963804 .. 8970421

Gene Structure 6618 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1086200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGGCTACTACTCCCGGAATTCTCACCGACTGGCCATGGACTCCTCTTGGAAGCTTTAAGCATGTGGTCGTGGCTCCTTGGATCATTCATGGTGCATACTCGTATTTCGTTAACGATGAGAAAGATAAAGACCTATCTTATTTCCTCATATTTCCATTTATGCTGTGGAGGTTTCTCCACAACCAGTTATGGATCTCTCTTTCTCGATATCGGACGGCCAAGGGCAATGGCCGGATTGTTGACAAGGGTCTTGAATTCGAGCAAGTCGACAGAGAAAGAAACTGGGATGATCAAATATTGTTCAATGGAATTCTATTCTATCTGGGGAGCAGACACTTACCTGGGGCTACGAACCTGCCATTTTGGAGGACAGATGGAGTTATTCTAACGATTCTACTTCATGCCGGTCCAGTGGAGTTTCTCTACTACTGGCTCCACAGAGCACTTCACCACCATTTCCTATACTCTCGCTACCACTCACATCACCATTCCTCCATTGTCACTGAGCCAATCACTTCTGTGATTCACCCATTTGCGGAGCACATCTCATACTTCGTACTCTTTGCTATACCAATGTTGACAACTATGTACACCGGGACATCTTCTATCGTAAGCTTTGCTGGTTATGTAACTTATATCGACTTTATGAACAACATGGGACATTGCAATTTTGAGCTCATTCCAAAGTGGCTCTTCACCATTTTCCCTCCTCTCAAGTATCTCCTTTACACCCCATCGTACCATTCTTTGCACCACACGCAATTCAGGACCAATTACTGTCTCTTCATGCCAGTCTACGACTACGTATATGGCACCATGGACAAATCAAGCGATTCACTCTATGAAACTTCCCTCAAGAGGGAGGAGGAATCGCCAGATGTCCTCCATCTAACCCATCTAACCACACCCGAGTCCATATATCATCTACCGCTAGGATTTGCTGCCTTAGCCTCTAGGCCCCACACAAGGACGTGGTACTTGTGGCTGATGTGGCCTGTGACATTGTGGTCTATGATGATAACTTGGATCCACGGCCGTACATTTGTTGTTGACAGGCACCGCTTTGACAAGCTCAGATTACAGACTTGGGCTATTCCCAAGTACAACTTTGCAAGTGAGGCCCCATCTACCTTTATGCAATGGCAAAATGAAGCTATCAATGGTTTGATTGAGGACGCCATCCTTGACGCAGAGGAAAAGGGTGTCAAGGTTTTAAGCCTAGGTCTCTTCAATCAGGGTGAGGAGTTAAATAGATATGGCGGTCTCTACGTTCAAAGGCATCCAAATCTCAAAATCAAGGTGGTGGATGGAAGGACAACGCAAGTTGTTCTTAGAGGCAAACTCACAAAGGTTGCTTATGCCATTGCCTATGCTTTGAGCCAGAAGGGTGTCCAGATAACTACATTATACCAGACTGAGTATTTGAAGCTCACCAGATCACTAGGCACTGAAAGTAATTTGGTTGTTGCAAAAAGCCACGCCCAAAAGATATGGTTGGTGGGTGATGGACTGACCGAGAAAGAACAGATGAGCGCACCAAAAGGAACATTATTTGTTCCCTTCTCACAATTCCCACCCAAAAAATTGCGCAAGGACTGCCTCTATCACTACACTCCAGCAATGAAGACTCCCGCATCTCTTGAGAATGTTCACGCTTGCGAGAACTGGTTGCCAAGAAGGGTGATGAGTGCATGGCGAGTAGCAGGAATAGTGCATGCCTTGGAAGGTTGGAAGGAGCATGACTGTGGTTACACCATGTCCAACATCGACAAATTTTGGCAAGCAAGTCTTGTGCATGGGTTTCAGCCTCTGACGATCAGTACCATTCACACCAAAAGTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016491 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko00073 ko01110 map00073 map01110

Pfam Domains

Protein Families

FA_hydroxylase Wax2_C

Protein Analysis

615

Amino Acids

71.3

Weight (kDa)

8.74

Isoelectric Point (pI)

33.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FA_hydroxylase	PF04116	134 - 272	1.6e-20	Fatty acid hydroxylase
CER1-like_C	PF12076	442 - 603	1.8e-71	CER1-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca	FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica	MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica	Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis	pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis	RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata	RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora	Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii	Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa	Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha	Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana	Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1006
Acc16I	TGCGCA	1 cut(s)	1589
Acc36I	ACCTGC	1 cut(s)	363
AccB1I	GGYRCC	2 cut(s)	812, 1056
AccI	GTMKAC	2 cut(s)	267, 795
AciI	CCGC	5 cut(s)	536, 929, 1060, 1260, 1632
AclWI	GGATC	4 cut(s)	89, 200, 1021, 1034
AcoI	YGGCCR	4 cut(s)	32, 216, 229, 1033
AcsI	RAATTY	4 cut(s)	18, 254, 309, 1772
AcyI	GRCGYC	1 cut(s)	1176
AfaI	GTAC	7 cut(s)	558, 590, 740, 977, 1039, 1103, 1822
AfiI	CCNNNNNNNGG	4 cut(s)	535, 858, 859, 966
AgsI	TTSAA	5 cut(s)	254, 304, 1231, 1274, 1426
AjiI	CACGTC	1 cut(s)	972
AjnI	CCWGG	1 cut(s)	340
AleI	CACNNNNGTG	1 cut(s)	518
AluBI	AGCT	6 cut(s)	54, 615, 676, 1072, 1154, 1429
AluI	AGCT	6 cut(s)	54, 615, 676, 1072, 1154, 1429
Alw21I	GWGCWC	3 cut(s)	445, 543, 678
Alw26I	GTCTC	3 cut(s)	785, 1229, 1268
AlwI	GGATC	4 cut(s)	89, 200, 1021, 1034
AlwNI	CAGNNNCTG	1 cut(s)	1810
Ama87I	CYCGRG	1 cut(s)	908
AoxI	GGCC	7 cut(s)	32, 216, 229, 956, 992, 1033, 1119
ApeKI	GCWGC	1 cut(s)	941
ApoI	RAATTY	4 cut(s)	18, 254, 309, 1772
ArsI	GACNNNNNNTTYG	2 cut(s)	1405, 1437
AspA2I	CCTAGG	1 cut(s)	1219
AspLEI	GCGC	2 cut(s)	1535, 1590
AspS9I	GGNCC	4 cut(s)	407, 768, 957, 1120
AsuC2I	CCSGG	2 cut(s)	15, 595
AsuHPI	GGTGA	9 cut(s)	16, 440, 476, 518, 688, 1250, 1423, 1514, 1693
AvaI	CYCGRG	1 cut(s)	908
AvaII	GGWCC	2 cut(s)	407, 768
AvrII	CCTAGG	1 cut(s)	1219
BalI	TGGCCA	1 cut(s)	34
BamHI	GGATCC	1 cut(s)	1026
BanI	GGYRCC	2 cut(s)	812, 1056
BanII	GRGCYC	1 cut(s)	678
BbsI	GAAGAC	1 cut(s)	1631
Bbv12I	GWGCWC	3 cut(s)	445, 543, 678
BbvI	GCAGC	1 cut(s)	928
BccI	CCATC	8 cut(s)	368, 742, 894, 903, 1132, 1187, 1301, 1499
BceAI	ACGGC	3 cut(s)	231, 1020, 1048
BciT130I	CCWGG	1 cut(s)	342
BclI	TGATCA	1 cut(s)	289
BcnI	CCSGG	2 cut(s)	15, 595
BcoDI	GTCTC	3 cut(s)	785, 1229, 1268
BfaI	CTAG	4 cut(s)	932, 954, 1220, 1442
BfuAI	ACCTGC	1 cut(s)	363
BisI	GCNGC	1 cut(s)	942
BlnI	CCTAGG	1 cut(s)	1219
BlsI	GCNGC	1 cut(s)	943
Bme1390I	CCNGG	3 cut(s)	15, 342, 595
Bme18I	GGWCC	2 cut(s)	407, 768
BmeT110I	CYCGRG	1 cut(s)	908
BmgBI	CACGTC	1 cut(s)	972
BmgT120I	GGNCC	4 cut(s)	407, 768, 957, 1120
BmiI	GGNNCC	7 cut(s)	75, 434, 814, 959, 1028, 1058, 1122
BmrFI	CCNGG	3 cut(s)	15, 342, 595
BmrI	ACTGGG	1 cut(s)	292
BmsI	GCATC	2 cut(s)	1288, 1643
BmuI	ACTGGG	1 cut(s)	292
BoxI	GACNNNNGTC	1 cut(s)	246
BpiI	GAAGAC	1 cut(s)	1631
BplI	GAGNNNNNCTC	4 cut(s)	530, 562, 1326, 1358
BpmI	CTGGAG	1 cut(s)	1599
Bpu10I	CCTNAGC	1 cut(s)	946
BpuEI	CTTGAG	3 cut(s)	698, 839, 1661
BpuMI	CCSGG	2 cut(s)	15, 595
BsaAI	YACGTR	1 cut(s)	805
BsaHI	GRCGYC	1 cut(s)	1176
BsaI	GGTCTC	2 cut(s)	1229, 1268
BsaJI	CCNNGG	8 cut(s)	35, 77, 219, 341, 816, 1030, 1219, 1719
BsaXI	ACNNNNNCTCC	2 cut(s)	476, 506
Bsc4I	CCNNNNNNNGG	4 cut(s)	535, 858, 859, 966
Bse118I	RCCGGY	1 cut(s)	404
Bse1I	ACTGG	7 cut(s)	35, 184, 287, 410, 434, 791, 1670
Bse3DI	GCAATG	5 cut(s)	232, 661, 1145, 1362, 1626
BseBI	CCWGG	1 cut(s)	342
BseDI	CCNNGG	8 cut(s)	35, 77, 219, 341, 816, 1030, 1219, 1719
BseGI	GGATG	4 cut(s)	292, 1179, 1279, 1312
BseLI	CCNNNNNNNGG	4 cut(s)	535, 858, 859, 966
BseMI	GCAATG	5 cut(s)	232, 661, 1145, 1362, 1626
BseMII	CTCAG	3 cut(s)	495, 1087, 1407
BseNI	ACTGG	7 cut(s)	35, 184, 287, 410, 434, 791, 1670
BseRI	GAGGAG	4 cut(s)	33, 699, 878, 1256
BseX3I	CGGCCG	1 cut(s)	1033
BseXI	GCAGC	1 cut(s)	928
Bsh1285I	CGRYCG	1 cut(s)	1036
BshFI	GGCC	7 cut(s)	34, 218, 231, 958, 994, 1035, 1121
BshNI	GGYRCC	2 cut(s)	812, 1056
BsiEI	CGRYCG	1 cut(s)	1036
BsiHKAI	GWGCWC	3 cut(s)	445, 543, 678
BsiHKCI	CYCGRG	1 cut(s)	908
BsiSI	CCGG	4 cut(s)	15, 232, 405, 594
BslFI	GGGAC	2 cut(s)	610, 672
BslI	CCNNNNNNNGG	4 cut(s)	535, 858, 859, 966
BsmAI	GTCTC	3 cut(s)	785, 1229, 1268
BsmFI	GGGAC	2 cut(s)	610, 672
BsnI	GGCC	7 cut(s)	34, 218, 231, 958, 994, 1035, 1121
Bso31I	GGTCTC	2 cut(s)	1229, 1268
BsoBI	CYCGRG	1 cut(s)	908
Bsp1286I	GDGCHC	3 cut(s)	445, 543, 678
Bsp1407I	TGTACA	1 cut(s)	588
Bsp143I	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
Bsp19I	CCATGG	2 cut(s)	35, 816
BspACI	CCGC	5 cut(s)	536, 929, 1060, 1260, 1632
BspANI	GGCC	7 cut(s)	34, 218, 231, 958, 994, 1035, 1121
BspCNI	CTCAG	3 cut(s)	496, 1086, 1408
BspLI	GGNNCC	7 cut(s)	75, 434, 814, 959, 1028, 1058, 1122
BspMI	ACCTGC	1 cut(s)	363
BspPI	GGATC	4 cut(s)	89, 200, 1021, 1034
BspQI	GCTCTTC	1 cut(s)	698
BspT107I	GGYRCC	2 cut(s)	812, 1056
BspTNI	GGTCTC	2 cut(s)	1229, 1268
BsrDI	GCAATG	5 cut(s)	232, 661, 1145, 1362, 1626
BsrFI	RCCGGY	1 cut(s)	404
BsrGI	TGTACA	1 cut(s)	588
BsrI	ACTGG	7 cut(s)	35, 184, 287, 410, 434, 791, 1670
BssAI	RCCGGY	1 cut(s)	404
BssECI	CCNNGG	8 cut(s)	35, 77, 219, 341, 816, 1030, 1219, 1719
BssMI	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
BssNI	GRCGYC	1 cut(s)	1176
BssT1I	CCWWGG	6 cut(s)	35, 77, 219, 816, 1219, 1719
Bst2UI	CCWGG	1 cut(s)	342
Bst4CI	ACNGT	2 cut(s)	779, 1745
Bst6I	CTCTTC	3 cut(s)	698, 788, 1232
BstACI	GRCGYC	1 cut(s)	1176
BstAPI	GCANNNNNTGC	1 cut(s)	1596
BstAUI	TGTACA	1 cut(s)	588
BstBAI	YACGTR	1 cut(s)	805
BstC8I	GCNNGC	3 cut(s)	1657, 1716, 1782
BstDEI	CTNAG	5 cut(s)	504, 946, 1073, 1331, 1416
BstDSI	CCRYGG	3 cut(s)	35, 816, 1030
BstF5I	GGATG	4 cut(s)	292, 1179, 1279, 1312
BstHHI	GCGC	2 cut(s)	1535, 1590
BstKTI	GATC	6 cut(s)	84, 195, 292, 1029, 1439, 1818
BstMAI	GTCTC	3 cut(s)	785, 1229, 1268
BstMBI	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
BstMCI	CGRYCG	1 cut(s)	1036
BstMWI	GCNNNNNNNGC	5 cut(s)	757, 947, 991, 1118, 1596
BstNI	CCWGG	1 cut(s)	342
BstNSI	RCATGY	2 cut(s)	65, 1718
BstPAI	GACNNNNGTC	1 cut(s)	246
BstSCI	CCNGG	3 cut(s)	13, 340, 593
BstSNI	TACGTA	1 cut(s)	805
BstV1I	GCAGC	1 cut(s)	928
BstV2I	GAAGAC	1 cut(s)	1631
BstX2I	RGATCY	2 cut(s)	192, 1026
BstYI	RGATCY	2 cut(s)	192, 1026
BstZI	CGGCCG	1 cut(s)	1033
BsuRI	GGCC	7 cut(s)	34, 218, 231, 958, 994, 1035, 1121
BtgI	CCRYGG	3 cut(s)	35, 816, 1030
BtrI	CACGTC	1 cut(s)	972
BtsCI	GGATG	4 cut(s)	292, 1179, 1279, 1312
BtsIMutI	CAGTG	3 cut(s)	417, 501, 1446
BveI	ACCTGC	1 cut(s)	363
Cac8I	GCNNGC	3 cut(s)	1657, 1716, 1782
CaiI	CAGNNNCTG	1 cut(s)	1810
CfoI	GCGC	2 cut(s)	1535, 1590
Cfr10I	RCCGGY	1 cut(s)	404
Cfr13I	GGNCC	4 cut(s)	407, 768, 957, 1120
CseI	GACGC	2 cut(s)	1184, 1196
Csp6I	GTAC	7 cut(s)	557, 589, 739, 976, 1038, 1102, 1821
CviQI	GTAC	7 cut(s)	557, 589, 739, 976, 1038, 1102, 1821
DdeI	CTNAG	5 cut(s)	504, 946, 1073, 1331, 1416
DpnI	GATC	6 cut(s)	83, 194, 291, 1028, 1438, 1817
DpnII	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
DrdI	GACNNNNNNGTC	1 cut(s)	1006
DseDI	GACNNNNNNGTC	1 cut(s)	1006
EaeI	YGGCCR	4 cut(s)	32, 216, 229, 1033
EagI	CGGCCG	1 cut(s)	1033
Eam1104I	CTCTTC	3 cut(s)	698, 788, 1232
EarI	CTCTTC	3 cut(s)	698, 788, 1232
Ecl136II	GAGCTC	1 cut(s)	676
EclXI	CGGCCG	1 cut(s)	1033
Eco105I	TACGTA	1 cut(s)	805
Eco130I	CCWWGG	6 cut(s)	35, 77, 219, 816, 1219, 1719
Eco24I	GRGCYC	1 cut(s)	678
Eco31I	GGTCTC	2 cut(s)	1229, 1268
Eco32I	GATATC	1 cut(s)	209
Eco47I	GGWCC	2 cut(s)	407, 768
Eco52I	CGGCCG	1 cut(s)	1033
Eco53kI	GAGCTC	1 cut(s)	676
Eco88I	CYCGRG	1 cut(s)	908
EcoICRI	GAGCTC	1 cut(s)	676
EcoO109I	RGGNCCY	2 cut(s)	957, 1120
EcoRI	GAATTC	3 cut(s)	18, 254, 309
EcoRII	CCWGG	1 cut(s)	340
EcoRV	GATATC	1 cut(s)	209
EcoT14I	CCWWGG	6 cut(s)	35, 77, 219, 816, 1219, 1719
EcoT38I	GRGCYC	1 cut(s)	678
ErhI	CCWWGG	6 cut(s)	35, 77, 219, 816, 1219, 1719
FalI	AAGNNNNNCTT	8 cut(s)	677, 709, 1091, 1123, 1314, 1346, 1773, 1805
FaqI	GGGAC	2 cut(s)	610, 672
FauI	CCCGC	1 cut(s)	1639
FbaI	TGATCA	1 cut(s)	289
FblI	GTMKAC	2 cut(s)	267, 795
Fnu4HI	GCNGC	1 cut(s)	942
FokI	GGATG	4 cut(s)	299, 1166, 1266, 1319
FriOI	GRGCYC	1 cut(s)	678
Fsp4HI	GCNGC	1 cut(s)	942
FspBI	CTAG	4 cut(s)	932, 954, 1220, 1442
FspI	TGCGCA	1 cut(s)	1589
GlaI	GCGC	2 cut(s)	1534, 1589
GluI	GCNGC	1 cut(s)	942
GsuI	CTGGAG	1 cut(s)	1599
HaeIII	GGCC	7 cut(s)	34, 218, 231, 958, 994, 1035, 1121
HapII	CCGG	4 cut(s)	15, 232, 405, 594
HgaI	GACGC	2 cut(s)	1184, 1196
HhaI	GCGC	2 cut(s)	1535, 1590
Hin1I	GRCGYC	1 cut(s)	1176
Hin6I	GCGC	2 cut(s)	1533, 1588
HinP1I	GCGC	2 cut(s)	1533, 1588
HincII	GTYRAC	5 cut(s)	112, 241, 268, 579, 1051
HindII	GTYRAC	5 cut(s)	112, 241, 268, 579, 1051
HindIII	AAGCTT	2 cut(s)	52, 613
HinfI	GANTC	7 cut(s)	40, 391, 523, 833, 869, 911, 1627
HpaI	GTTAAC	1 cut(s)	112
HpaII	CCGG	4 cut(s)	15, 232, 405, 594
HphI	GGTGA	9 cut(s)	16, 440, 476, 518, 688, 1250, 1423, 1514, 1693
Hpy166II	GTNNAC	8 cut(s)	112, 241, 268, 579, 591, 796, 1051, 1651
Hpy188I	TCNGA	3 cut(s)	213, 1076, 1812
Hpy188III	TCNNGA	6 cut(s)	204, 251, 766, 856, 1393, 1640
Hpy8I	GTNNAC	8 cut(s)	112, 241, 268, 579, 591, 796, 1051, 1651
HpyAV	CCTTC	6 cut(s)	1305, 1378, 1570, 1671, 1718, 1726
HpyCH4III	ACNGT	2 cut(s)	779, 1745
HpyCH4IV	ACGT	3 cut(s)	804, 971, 1269
HpyCH4V	TGCA	9 cut(s)	95, 666, 751, 1112, 1138, 1469, 1691, 1714, 1795
HpyF10VI	GCNNNNNNNGC	5 cut(s)	757, 947, 991, 1118, 1596
HpyF3I	CTNAG	5 cut(s)	504, 946, 1073, 1331, 1416
HpySE526I	ACGT	3 cut(s)	804, 971, 1269
Hsp92I	GRCGYC	1 cut(s)	1176
HspAI	GCGC	2 cut(s)	1533, 1588
Ksp22I	TGATCA	1 cut(s)	289
KspAI	GTTAAC	1 cut(s)	112
Kzo9I	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
LguI	GCTCTTC	1 cut(s)	698
LmnI	GCTCC	5 cut(s)	79, 327, 438, 538, 1735
Lsp1109I	GCAGC	1 cut(s)	928
LweI	GCATC	2 cut(s)	1288, 1643
MaeI	CTAG	4 cut(s)	932, 954, 1220, 1442
MaeII	ACGT	3 cut(s)	804, 971, 1269
MaeIII	GTNAC	4 cut(s)	499, 628, 997, 1748
MalI	GATC	6 cut(s)	83, 194, 291, 1028, 1438, 1817
MboI	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
MboII	GAAGA	5 cut(s)	594, 685, 775, 1219, 1636
MflI	RGATCY	2 cut(s)	192, 1026
MhlI	GDGCHC	3 cut(s)	445, 543, 678
MlsI	TGGCCA	1 cut(s)	34
MluNI	TGGCCA	1 cut(s)	34
MlyI	GAGTC	3 cut(s)	34, 920, 1621
MmeI	TCCRAC	2 cut(s)	1709, 1785
Mox20I	TGGCCA	1 cut(s)	34
MscI	TGGCCA	1 cut(s)	34
MseI	TTAA	4 cut(s)	57, 111, 1214, 1247
MslI	CAYNNNNRTG	2 cut(s)	518, 1743
Msp20I	TGGCCA	1 cut(s)	34
MspI	CCGG	4 cut(s)	15, 232, 405, 594
MspR9I	CCNGG	3 cut(s)	15, 342, 595
MvaI	CCWGG	1 cut(s)	342
MwoI	GCNNNNNNNGC	5 cut(s)	757, 947, 991, 1118, 1596
NciI	CCSGG	2 cut(s)	15, 595
NcoI	CCATGG	2 cut(s)	35, 816
NdeII	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
NlaIV	GGNNCC	7 cut(s)	75, 434, 814, 959, 1028, 1058, 1122
NmuCI	GTSAC	2 cut(s)	499, 997
NsbI	TGCGCA	1 cut(s)	1589
NspI	RCATGY	2 cut(s)	65, 1718
OliI	CACNNNNGTG	1 cut(s)	518
PaeI	GCATGC	1 cut(s)	1718
PciSI	GCTCTTC	1 cut(s)	698
PcsI	WCGNNNNNNNCGW	1 cut(s)	264
PfeI	GAWTC	4 cut(s)	391, 523, 833, 869
PfoI	TCCNGGA	1 cut(s)	13
PkrI	GCNGC	1 cut(s)	943
PleI	GAGTC	3 cut(s)	34, 919, 1621
PpsI	GAGTC	3 cut(s)	34, 919, 1621
Ppu21I	YACGTR	1 cut(s)	805
PshAI	GACNNNNGTC	1 cut(s)	246
Psp124BI	GAGCTC	1 cut(s)	678
Psp6I	CCWGG	1 cut(s)	340
PspGI	CCWGG	1 cut(s)	340
PspN4I	GGNNCC	7 cut(s)	75, 434, 814, 959, 1028, 1058, 1122
PspPI	GGNCC	4 cut(s)	407, 768, 957, 1120
PstNI	CAGNNNCTG	1 cut(s)	1810
PsuI	RGATCY	2 cut(s)	192, 1026
RsaI	GTAC	7 cut(s)	558, 590, 740, 977, 1039, 1103, 1822
RsaNI	GTAC	7 cut(s)	557, 589, 739, 976, 1038, 1102, 1821
RseI	CAYNNNNRTG	2 cut(s)	518, 1743
SacI	GAGCTC	1 cut(s)	678
SalI	GTCGAC	1 cut(s)	266
SapI	GCTCTTC	1 cut(s)	698
SaqAI	TTAA	4 cut(s)	57, 111, 1214, 1247
SatI	GCNGC	1 cut(s)	942
Sau3AI	GATC	6 cut(s)	81, 192, 289, 1026, 1436, 1815
Sau96I	GGNCC	4 cut(s)	407, 768, 957, 1120
SchI	GAGTC	3 cut(s)	34, 920, 1621
ScrFI	CCNGG	3 cut(s)	15, 342, 595
SduI	GDGCHC	3 cut(s)	445, 543, 678
SfaNI	GCATC	2 cut(s)	1288, 1643
SinI	GGWCC	2 cut(s)	407, 768
SmiMI	CAYNNNNRTG	2 cut(s)	518, 1743
SmlI	CTYRAG	3 cut(s)	713, 854, 1640
SmoI	CTYRAG	3 cut(s)	713, 854, 1640
SnaBI	TACGTA	1 cut(s)	805
SphI	GCATGC	1 cut(s)	1718
SsiI	CCGC	5 cut(s)	536, 929, 1060, 1260, 1632
SspI	AATATT	1 cut(s)	297
SspMI	CTAG	4 cut(s)	932, 954, 1220, 1442
SstI	GAGCTC	1 cut(s)	678
StyD4I	CCNGG	3 cut(s)	13, 340, 593
StyI	CCWWGG	6 cut(s)	35, 77, 219, 816, 1219, 1719
TaaI	ACNGT	2 cut(s)	779, 1745
TaiI	ACGT	3 cut(s)	807, 974, 1272
TaqI	TCGA	5 cut(s)	205, 258, 267, 639, 1767
TaqII	GACCGA	1 cut(s)	1529
TatI	WGTACW	2 cut(s)	588, 1101
TfiI	GAWTC	4 cut(s)	391, 523, 833, 869
Tru1I	TTAA	4 cut(s)	57, 111, 1214, 1247
Tru9I	TTAA	4 cut(s)	57, 111, 1214, 1247
TscAI	CASTG	3 cut(s)	417, 508, 1453
TseFI	GTSAC	2 cut(s)	499, 997
TseI	GCWGC	1 cut(s)	941
Tsp45I	GTSAC	2 cut(s)	499, 997
TspDTI	ATGAA	7 cut(s)	77, 389, 662, 775, 858, 1164, 1637
TspRI	CASTG	3 cut(s)	417, 508, 1453
VpaK11BI	GGWCC	2 cut(s)	407, 768
XapI	RAATTY	4 cut(s)	18, 254, 309, 1772
XceI	RCATGY	2 cut(s)	65, 1718
XcmI	CCANNNNNNNNNTGG	1 cut(s)	162
XmaJI	CCTAGG	1 cut(s)	1219
XmiI	GTMKAC	2 cut(s)	267, 795
XspI	CTAG	4 cut(s)	932, 954, 1220, 1442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.