FvH4_7g08590

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000300
Physical Location & Seq
Forward (+)
8448262 .. 8452398
4137 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08590.t1

Sequence Viewer

Length: 1872 bp
ATGGCTTCCAATCCTGGAATTCTGACTGAATGGCCATGGACACGTCTTGGAAGCTTTAAGTACGTGGTGGTGGCTCCCTTCGCGATACACAGCACATATTCATTCATTGTTAACGAATGGGAAGACAAAGATCTGTCTTACTTCCTCATATTTCCATACATGTTGTTTAGGATGCTGCACAACCAGCTATGGATCTCTCTTTCTCGATATCGAACCTCCAAGGGCAGCGGCCGGATCATCGACAAGGGCCTTGAGTTCGAACAAGTTGACAGAGAAAGAAACTGGGATGACCAAATTATATTCAATGCAATATTGTTCTACATAGGGAGAATGCGGCTGGACGGAGGTCGAAACGTACCACTGTGGAGAGCAGATGGATTCGTCATAACATTTCTGCTTCATGCTGGTCCGGTGGAGTTTCTCTACTACTGGCTGCATAGAGCTCTGCACCACCATTACCTTTACTCTCGCTACCATTCTCACCACCATTCCTCCATTGTCACTGAGCCAATTACTTCTGTAATTCACCCATTTGGGGAGCACATAGCATATTTCATCCTTTTCGCAATACCCATGATGACAATGGCGTTGACGAGAACAGCCTCAATCATAACCTTTGCAGTTTATATTACTTACATTGACGCTATGAACAACATGGGACACTGCAACTTCGAGCTCATTCCAAAATGGATCTTCTCTCTTATTCCTCCACTCAAATATTTCATGTATACACCATCGTATCACTCTTTGCATCACACCCTATTTCGGACCAATTACTGCCTCTTCATGCCAATCTATGACTACATGTATGGCACCATGGAAAAGACCAGTGATGAACTGCATGAATCTTCACTCAAAAGAAAAGATGAAGCTCCGGATGTCTTGCATTTAACCCATCTTACAACACCCGACTCCATCTATCAACTTCCGCTCGGATTTGCTTCCTTGGCCTCTAAACCGCACACTTCAACGTGGTACTTGTGGTTGATGTGGCCAGTCACATTTTGGTCGATGATGCTCACTTGGATTTATGGTCGCACATTTGTGGTCGAGAGGCAGCGATTTAATAAACTCAAAATACAAACTTGGGCGATCCCAAAATACAATTTGCAATACTTCATGCCATGGCAAAATGAAGCTATCAACAGTTTGATTGAGGACGCTATACTTGAAGCAGAGGAAAAAGGTGTCAAAGTCGTAAGTTTGGGGCTCTTAAACCAGGCTGAGGAGCTGAATAGATATGGCGGTCTATATACTCGGAGATATCCTCAGCTGAAAGTGAAGCTAGTGGATGGAAGTAGCTTAGCTGCTGCTGTAATCCTCAACAACATACCAAAAGAGACAACCCAAGTTGTTCTTACAGGCAACTTTACAAAGGTTGCTTATGCTATTGCATTTGCTTTGAGCCAGAGAGGCATTGAGGTAGCTACAATATACAAGGATGAGTATTTGAAGCTCACCAAATCGTTAAGTGCTACTAAGAGTACGGTGGTTCTTGCAAAAGGCTCTGCTTCAAAGATTTGGTTAGTGGGAGATGGATTGAGTAAACAAACACAGCTACAAGCACCAAAAGGAACAATCTTTGTTCCTTTTACCCAATTCCCCCTAAAAGAGCTGCGCAGGGACTGCTTCTACCATTGCCCTCCAGCAATGAAGATCCCTAAGTCTATTGAGAATGTGTACTCGTGTGAGAACTGGTTGCCGAGAAGGGTGATGAGCGCATGGCGCATAGCCGGAATAGTGCATGCCTCGGAAGGTTGGAATGAACATGAGTGTGGATACGCCATGTCCGACATTGACAAGGTCTGGGAAGCTACTCTTCGACTTGGCTTTCAGCCTTTGATCCCCAATGCTACAGCAAACAAATCTTAA

Protein Analysis

624

Amino Acids

72.29

Weight (kDa)

8.86

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 133 - 272 8.5e-20 Fatty acid hydroxylase
CER1-like_C PF12076 451 - 613 7.5e-68 CER1-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02190 AT1G02190 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT1G02205 AT2G37700 AT2G37700 AT2G37700 AT2G37700
fragaria_vesca FvH4_1g22160 FvH4_7g08560 FvH4_7g08560 FvH4_7g08560 FvH4_7g08570 FvH4_7g08570 FvH4_7g08590 FvH4_7g08590
malus_domestica MD02G1226900.v1.1 MD07G1083700.v1.1 MD07G1083800.v1.1 MD07G1086200.v1.1 MD07G1086300.v1.1 MD07G1086700.v1.1 MD13G1075600.v1.1
prunus_persica Prupe.2G112600_v2.0.a1 Prupe.2G112800_v2.0.a1 Prupe.2G113000_v2.0.a1 Prupe.2G113500_v2.0.a1 Prupe.2G113600_v2.0.a1
pyrus_communis pycom02g19600 pycom02g19610 pycom07g06920 pycom07g06930
rosa_chinensis RchiOBHm_Chr1g0343261 RchiOBHm_Chr1g0343301 RchiOBHm_Chr1g0343371 RchiOBHm_Chr1g0343431 RchiOBHm_Chr1g0343441 RchiOBHm_Chr1g0343521 RchiOBHm_Chr1g0343691 RchiOBHm_Chr1g0343791
rosa_laevigata RLG00000028951 RLG00000028953 RLG00000028965 RLG00000028968 RLG00000028969 RLG00000028971 RLG00000028972 RLG00000028974 RLG00000029891
rosa_multiflora Rmu_sc0000021.1_g000002 Rmu_sc0000335.1_g000046 Rmu_sc0000335.1_g000047 Rmu_sc0000335.1_g000058 Rmu_sc0000335.1_g000061 Rmu_sc0000335.1_g000106 Rmu_sc0000335.1_g000125 Rmu_sc0000544.1_g000010 Rmu_sc0003430.1_g000012 Rmu_sc0005796.1_g000004 Rmu_sc0028268.1_g000001 Rmu_ssc0000259.1_g000038
rosa_roxburghii Rroxscaffold_2G00110090 Rroxscaffold_4G00310350 Rroxscaffold_4G00310610 Rroxscaffold_4G00310710 Rroxscaffold_4G00310740 Rroxscaffold_4G00310760 Rroxscaffold_4G00321110
rosa_rugosa Rorug01G0080100 Rorug01G0080200 Rorug01G0166400 Rorug01G0166500 Rorug01G0166500 Rorug01G0166600 Rorug01G0166700 Rorug01G0166800 Rorug01G0168400 Rorug01G0168500 Rorug01G0168800 Rorug03G0241300 Rorug03G0241400 Rorug04G0001200 Rorug06G0031500 Rorug06G0073200 Rorug06G0264000
rosa_samantha Rh1AG098100 Rh1AG181800 Rh1AG182100 Rh1AG182400 Rh1AG183000 Rh1AG183600 Rh1AG184300 Rh1AG185400 Rh1BG104000 Rh1BG150000 Rh1BG150400 Rh1BG152100 Rh1BG152300 Rh1CG168600 Rh1CG168900 Rh1CG169000 Rh1CG169700 Rh1CG170500 Rh1CG171200 Rh1DG182000 Rh1DG182300 Rh1DG184000 Rh1DG184200
rosa_wichuraiana Rw0G012940 Rw1G007640 Rw1G007670 Rw1G011320 Rw1G015090 Rw1G015110 Rw1G015130 Rw1G015150 Rw1G015290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1619
AccB1I GGYRCC 1 cut(s) 812
AccBSI CCGCTC 1 cut(s) 931
AccI GTMKAC 1 cut(s) 728
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 874
AciI CCGC 5 cut(s) 228, 334, 929, 959, 1245
AclWI GGATC 6 cut(s) 200, 242, 698, 1087, 1651, 1837
AcoI YGGCCR 3 cut(s) 32, 229, 992
AcsI RAATTY 1 cut(s) 18
AfaI GTAC 5 cut(s) 62, 357, 977, 1486, 1682
AfiI CCNNNNNNNGG 3 cut(s) 535, 765, 1225
AflIII ACRYGT 3 cut(s) 41, 159, 804
AgsI TTSAA 5 cut(s) 304, 969, 1172, 1453, 1515
AjiI CACGTC 1 cut(s) 44
AjnI CCWGG 2 cut(s) 13, 1218
AleI CACNNNNGTG 1 cut(s) 1043
Alw21I GWGCWC 3 cut(s) 445, 543, 678
Alw26I GTCTC 1 cut(s) 1334
AlwI GGATC 6 cut(s) 200, 242, 698, 1087, 1651, 1837
AlwNI CAGNNNCTG 1 cut(s) 1626
Aor13HI TCCGGA 1 cut(s) 874
AoxI GGCC 5 cut(s) 32, 229, 247, 948, 992
ApeKI GCWGC 7 cut(s) 175, 225, 433, 1057, 1307, 1310, 1615
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 3 cut(s) 1620, 1721, 1728
AspS9I GGNCC 3 cut(s) 247, 407, 768
AsuHPI GGTGA 4 cut(s) 473, 518, 1450, 1723
AsuII TTCGAA 1 cut(s) 258
AvaII GGWCC 2 cut(s) 407, 768
BaeI ACNNNNGTAYC 2 cut(s) 722, 755
BalI TGGCCA 2 cut(s) 34, 994
BanI GGYRCC 1 cut(s) 812
BanII GRGCYC 3 cut(s) 445, 678, 1212
BauI CACGAG 1 cut(s) 1684
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 3 cut(s) 445, 543, 678
BbvCI CCTCAGC 2 cut(s) 1224, 1269
BbvI GCAGC 7 cut(s) 162, 237, 420, 1069, 1294, 1297, 1602
BccI CCATC 6 cut(s) 368, 742, 903, 923, 1286, 1529
BciT130I CCWGG 2 cut(s) 15, 1220
BciVI GTATCC 1 cut(s) 1772
BcoDI GTCTC 1 cut(s) 1334
BfaI CTAG 1 cut(s) 1286
BfmI CTRYAG 1 cut(s) 1854
BfuI GTATCC 1 cut(s) 1772
BglI GCCNNNNNGGC 1 cut(s) 1413
BglII AGATCT 1 cut(s) 130
BisI GCNGC 9 cut(s) 176, 226, 229, 335, 434, 1058, 1308, 1311, 1616
BlpI GCTNAGC 1 cut(s) 1303
BlsI GCNGC 9 cut(s) 177, 227, 230, 336, 435, 1059, 1309, 1312, 1617
Bme1390I CCNGG 2 cut(s) 15, 1220
Bme18I GGWCC 2 cut(s) 407, 768
BmgBI CACGTC 1 cut(s) 44
BmgT120I GGNCC 3 cut(s) 247, 407, 768
BmiI GGNNCC 2 cut(s) 75, 814
BmrFI CCNGG 2 cut(s) 15, 1220
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 3 cut(s) 162, 760, 1005
BmuI ACTGGG 1 cut(s) 292
BoxI GACNNNNGTC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 129
BplI GAGNNNNNCTC 2 cut(s) 1252, 1284
BpmI CTGGAG 1 cut(s) 1629
Bpu10I CCTNAGC 2 cut(s) 1224, 1269
Bpu1102I GCTNAGC 1 cut(s) 1303
Bpu14I TTCGAA 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 64
BsaJI CCNNGG 6 cut(s) 35, 219, 816, 945, 1124, 1749
BsaWI WCCGGW 2 cut(s) 409, 874
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 535, 765, 1225
Bse1I ACTGG 5 cut(s) 287, 434, 828, 995, 1700
Bse3DI GCAATG 2 cut(s) 1636, 1656
BseAI TCCGGA 1 cut(s) 874
BseBI CCWGG 2 cut(s) 15, 1220
BseDI CCNNGG 6 cut(s) 35, 219, 816, 945, 1124, 1749
BseGI GGATG 6 cut(s) 177, 292, 555, 883, 1297, 1447
BseLI CCNNNNNNNGG 3 cut(s) 535, 765, 1225
BseMI GCAATG 2 cut(s) 1636, 1656
BseMII CTCAG 3 cut(s) 495, 1215, 1283
BseNI ACTGG 5 cut(s) 287, 434, 828, 995, 1700
BseRI GAGGAG 1 cut(s) 1241
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 7 cut(s) 162, 237, 420, 1069, 1294, 1297, 1602
BsgI GTGCAG 2 cut(s) 161, 431
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 5 cut(s) 34, 231, 249, 950, 994
BshNI GGYRCC 1 cut(s) 812
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 3 cut(s) 445, 543, 678
BsiSI CCGG 4 cut(s) 232, 410, 875, 1734
BslFI GGGAC 2 cut(s) 672, 1637
BslI CCNNNNNNNGG 3 cut(s) 535, 765, 1225
BsmAI GTCTC 1 cut(s) 1334
BsmFI GGGAC 2 cut(s) 672, 1637
BsmI GAATGC 1 cut(s) 336
BsnI GGCC 5 cut(s) 34, 231, 249, 950, 994
Bsp119I TTCGAA 1 cut(s) 258
Bsp1286I GDGCHC 4 cut(s) 445, 543, 678, 1212
Bsp13I TCCGGA 1 cut(s) 874
Bsp143I GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
Bsp1720I GCTNAGC 1 cut(s) 1303
Bsp19I CCATGG 3 cut(s) 35, 816, 1124
Bsp68I TCGCGA 1 cut(s) 83
BspACI CCGC 5 cut(s) 228, 334, 929, 959, 1245
BspANI GGCC 5 cut(s) 34, 231, 249, 950, 994
BspCNI CTCAG 3 cut(s) 496, 1216, 1282
BspEI TCCGGA 1 cut(s) 874
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 2 cut(s) 75, 814
BspPI GGATC 6 cut(s) 200, 242, 698, 1087, 1651, 1837
BspT104I TTCGAA 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 812
BsrBI CCGCTC 1 cut(s) 931
BsrDI GCAATG 2 cut(s) 1636, 1656
BsrI ACTGG 5 cut(s) 287, 434, 828, 995, 1700
BssECI CCNNGG 6 cut(s) 35, 219, 816, 945, 1124, 1749
BssMI GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
BssNAI GTATAC 1 cut(s) 729
BssSI CACGAG 1 cut(s) 1684
BssT1I CCWWGG 5 cut(s) 35, 219, 816, 945, 1124
Bst1107I GTATAC 1 cut(s) 729
Bst2BI CACGAG 1 cut(s) 1684
Bst2UI CCWGG 2 cut(s) 15, 1220
Bst4CI ACNGT 3 cut(s) 363, 1148, 1489
Bst6I CTCTTC 2 cut(s) 788, 1824
BstAPI GCANNNNNTGC 1 cut(s) 1626
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 1746
BstDEI CTNAG 6 cut(s) 504, 1224, 1269, 1303, 1479, 1662
BstDSI CCRYGG 3 cut(s) 35, 816, 1124
BstF5I GGATG 6 cut(s) 177, 292, 555, 883, 1297, 1447
BstFNI CGCG 1 cut(s) 83
BstHHI GCGC 3 cut(s) 1620, 1721, 1728
BstKTI GATC 7 cut(s) 133, 195, 237, 693, 1095, 1659, 1845
BstMAI GTCTC 1 cut(s) 1334
BstMBI GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 6 cut(s) 80, 184, 947, 1413, 1626, 1725
BstNI CCWGG 2 cut(s) 15, 1220
BstNSI RCATGY 3 cut(s) 163, 808, 1748
BstPAI GACNNNNGTC 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 13, 1218
BstSFI CTRYAG 1 cut(s) 1854
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 7 cut(s) 162, 237, 420, 1069, 1294, 1297, 1602
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 4 cut(s) 130, 192, 690, 1656
BstYI RGATCY 4 cut(s) 130, 192, 690, 1656
BstZ17I GTATAC 1 cut(s) 729
BstZI CGGCCG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 1772
BsuRI GGCC 5 cut(s) 34, 231, 249, 950, 994
BtgI CCRYGG 3 cut(s) 35, 816, 1124
BtrI CACGTC 1 cut(s) 44
BtsCI GGATG 6 cut(s) 177, 292, 555, 883, 1297, 1447
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 4 cut(s) 359, 501, 661, 835
BtuMI TCGCGA 1 cut(s) 83
Cac8I GCNNGC 1 cut(s) 1746
CaiI CAGNNNCTG 1 cut(s) 1626
CfoI GCGC 3 cut(s) 1620, 1721, 1728
Cfr13I GGNCC 3 cut(s) 247, 407, 768
CseI GACGC 2 cut(s) 650, 1169
Csp6I GTAC 5 cut(s) 61, 356, 976, 1485, 1681
CviQI GTAC 5 cut(s) 61, 356, 976, 1485, 1681
DdeI CTNAG 6 cut(s) 504, 1224, 1269, 1303, 1479, 1662
DpnI GATC 7 cut(s) 132, 194, 236, 692, 1094, 1658, 1844
DpnII GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
EaeI YGGCCR 3 cut(s) 32, 229, 992
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 788, 1824
EarI CTCTTC 2 cut(s) 788, 1824
Ecl136II GAGCTC 2 cut(s) 443, 676
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 5 cut(s) 35, 219, 816, 945, 1124
Eco24I GRGCYC 3 cut(s) 445, 678, 1212
Eco32I GATATC 2 cut(s) 209, 1265
Eco47I GGWCC 2 cut(s) 407, 768
Eco52I CGGCCG 1 cut(s) 229
Eco53kI GAGCTC 2 cut(s) 443, 676
EcoICRI GAGCTC 2 cut(s) 443, 676
EcoO109I RGGNCCY 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 2 cut(s) 13, 1218
EcoRV GATATC 2 cut(s) 209, 1265
EcoT14I CCWWGG 5 cut(s) 35, 219, 816, 945, 1124
EcoT38I GRGCYC 3 cut(s) 445, 678, 1212
ErhI CCWWGG 5 cut(s) 35, 219, 816, 945, 1124
FalI AAGNNNNNCTT 4 cut(s) 1341, 1373, 1803, 1835
FaqI GGGAC 2 cut(s) 672, 1637
FblI GTMKAC 1 cut(s) 728
Fnu4HI GCNGC 9 cut(s) 176, 226, 229, 335, 434, 1058, 1308, 1311, 1616
FokI GGATG 6 cut(s) 184, 299, 542, 890, 1304, 1454
FriOI GRGCYC 3 cut(s) 445, 678, 1212
Fsp4HI GCNGC 9 cut(s) 176, 226, 229, 335, 434, 1058, 1308, 1311, 1616
FspBI CTAG 1 cut(s) 1286
FspI TGCGCA 1 cut(s) 1619
GlaI GCGC 3 cut(s) 1619, 1720, 1727
GluI GCNGC 9 cut(s) 176, 226, 229, 335, 434, 1058, 1308, 1311, 1616
GsuI CTGGAG 1 cut(s) 1629
HaeIII GGCC 5 cut(s) 34, 231, 249, 950, 994
HapII CCGG 4 cut(s) 232, 410, 875, 1734
HgaI GACGC 2 cut(s) 650, 1169
HhaI GCGC 3 cut(s) 1620, 1721, 1728
Hin6I GCGC 3 cut(s) 1618, 1719, 1726
HinP1I GCGC 3 cut(s) 1618, 1719, 1726
HincII GTYRAC 3 cut(s) 112, 268, 591
HindII GTYRAC 3 cut(s) 112, 268, 591
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 3 cut(s) 378, 845, 911
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 4 cut(s) 232, 410, 875, 1734
HphI GGTGA 4 cut(s) 473, 518, 1450, 1723
Hpy166II GTNNAC 6 cut(s) 112, 268, 591, 729, 1547, 1681
Hpy188I TCNGA 6 cut(s) 24, 768, 935, 1260, 1753, 1792
Hpy188III TCNNGA 4 cut(s) 82, 204, 875, 1051
Hpy8I GTNNAC 6 cut(s) 112, 268, 591, 729, 1547, 1681
HpyAV CCTTC 3 cut(s) 88, 1701, 1748
HpyCH4III ACNGT 3 cut(s) 363, 1148, 1489
HpyCH4IV ACGT 4 cut(s) 43, 63, 354, 971
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 184, 947, 1413, 1626, 1725
HpyF3I CTNAG 6 cut(s) 504, 1224, 1269, 1303, 1479, 1662
HpySE526I ACGT 4 cut(s) 43, 63, 354, 971
HspAI GCGC 3 cut(s) 1618, 1719, 1726
Kpn2I TCCGGA 1 cut(s) 874
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
LmnI GCTCC 4 cut(s) 79, 538, 877, 1228
Lsp1109I GCAGC 7 cut(s) 162, 237, 420, 1069, 1294, 1297, 1602
LweI GCATC 3 cut(s) 162, 760, 1005
MaeI CTAG 1 cut(s) 1286
MaeII ACGT 4 cut(s) 43, 63, 354, 971
MaeIII GTNAC 2 cut(s) 499, 997
MalI GATC 7 cut(s) 132, 194, 236, 692, 1094, 1658, 1844
MbiI CCGCTC 1 cut(s) 931
MboI GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
MboII GAAGA 6 cut(s) 134, 685, 775, 840, 1666, 1811
MflI RGATCY 4 cut(s) 130, 192, 690, 1656
MhlI GDGCHC 4 cut(s) 445, 543, 678, 1212
MlsI TGGCCA 2 cut(s) 34, 994
MluCI AATT 7 cut(s) 18, 294, 510, 522, 772, 1105, 1598
MluNI TGGCCA 2 cut(s) 34, 994
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 2 cut(s) 1739, 1815
Mox20I TGGCCA 2 cut(s) 34, 994
MroI TCCGGA 1 cut(s) 874
MscI TGGCCA 2 cut(s) 34, 994
MseI TTAA 7 cut(s) 57, 111, 890, 1065, 1214, 1469, 1870
MslI CAYNNNNRTG 2 cut(s) 1043, 1773
Msp20I TGGCCA 2 cut(s) 34, 994
MspA1I CMGCKG 2 cut(s) 228, 1273
MspI CCGG 4 cut(s) 232, 410, 875, 1734
MspR9I CCNGG 2 cut(s) 15, 1220
Mva1269I GAATGC 1 cut(s) 336
MvaI CCWGG 2 cut(s) 15, 1220
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 6 cut(s) 80, 184, 947, 1413, 1626, 1725
NcoI CCATGG 3 cut(s) 35, 816, 1124
NdeII GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
NlaIV GGNNCC 2 cut(s) 75, 814
NmeAIII GCCGAG 1 cut(s) 1728
NmuCI GTSAC 2 cut(s) 499, 997
NruI TCGCGA 1 cut(s) 83
NsbI TGCGCA 1 cut(s) 1619
NspI RCATGY 3 cut(s) 163, 808, 1748
NspV TTCGAA 1 cut(s) 258
OliI CACNNNNGTG 1 cut(s) 1043
PaeI GCATGC 1 cut(s) 1748
PciI ACATGT 2 cut(s) 159, 804
PcsI WCGNNNNNNNCGW 1 cut(s) 1788
PctI GAATGC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 378, 845
PflFI GACNNNGTC 1 cut(s) 1802
PfoI TCCNGGA 1 cut(s) 13
PkrI GCNGC 9 cut(s) 177, 227, 230, 336, 435, 1059, 1309, 1312, 1617
PleI GAGTC 1 cut(s) 905
PpsI GAGTC 1 cut(s) 905
Ppu21I YACGTR 1 cut(s) 64
PscI ACATGT 2 cut(s) 159, 804
PshAI GACNNNNGTC 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 445, 678
Psp6I CCWGG 2 cut(s) 13, 1218
PspGI CCWGG 2 cut(s) 13, 1218
PspN4I GGNNCC 2 cut(s) 75, 814
PspPI GGNCC 3 cut(s) 247, 407, 768
PstNI CAGNNNCTG 1 cut(s) 1626
PsuI RGATCY 4 cut(s) 130, 192, 690, 1656
PsyI GACNNNGTC 1 cut(s) 1802
PvuII CAGCTG 1 cut(s) 1273
RruI TCGCGA 1 cut(s) 83
RsaI GTAC 5 cut(s) 62, 357, 977, 1486, 1682
RsaNI GTAC 5 cut(s) 61, 356, 976, 1485, 1681
RseI CAYNNNNRTG 2 cut(s) 1043, 1773
SacI GAGCTC 2 cut(s) 445, 678
SaqAI TTAA 7 cut(s) 57, 111, 890, 1065, 1214, 1469, 1870
SatI GCNGC 9 cut(s) 176, 226, 229, 335, 434, 1058, 1308, 1311, 1616
Sau3AI GATC 7 cut(s) 130, 192, 234, 690, 1092, 1656, 1842
Sau96I GGNCC 3 cut(s) 247, 407, 768
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 15, 1220
SduI GDGCHC 4 cut(s) 445, 543, 678, 1212
SfaNI GCATC 3 cut(s) 162, 760, 1005
SfcI CTRYAG 1 cut(s) 1854
SfuI TTCGAA 1 cut(s) 258
SinI GGWCC 2 cut(s) 407, 768
SmiMI CAYNNNNRTG 2 cut(s) 1043, 1773
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SphI GCATGC 1 cut(s) 1748
Sse9I AATT 7 cut(s) 18, 294, 510, 522, 772, 1105, 1598
SsiI CCGC 5 cut(s) 228, 334, 929, 959, 1245
SspI AATATT 2 cut(s) 312, 719
SspMI CTAG 1 cut(s) 1286
SstI GAGCTC 2 cut(s) 445, 678
StyD4I CCNGG 2 cut(s) 13, 1218
StyI CCWWGG 5 cut(s) 35, 219, 816, 945, 1124
TaaI ACNGT 3 cut(s) 363, 1148, 1489
TaiI ACGT 4 cut(s) 46, 66, 357, 974
TaqI TCGA 9 cut(s) 205, 211, 240, 258, 349, 672, 1010, 1050, 1822
TasI AATT 7 cut(s) 18, 294, 510, 522, 772, 1105, 1598
TatI WGTACW 1 cut(s) 1680
TauI GCSGC 2 cut(s) 231, 337
TfiI GAWTC 2 cut(s) 378, 845
Tru1I TTAA 7 cut(s) 57, 111, 890, 1065, 1214, 1469, 1870
Tru9I TTAA 7 cut(s) 57, 111, 890, 1065, 1214, 1469, 1870
TscAI CASTG 4 cut(s) 366, 508, 668, 835
TseFI GTSAC 2 cut(s) 499, 997
TseI GCWGC 7 cut(s) 175, 225, 433, 1057, 1307, 1310, 1615
Tsp45I GTSAC 2 cut(s) 499, 997
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 4 cut(s) 366, 508, 668, 835
Tth111I GACNNNGTC 1 cut(s) 1802
VpaK11BI GGWCC 2 cut(s) 407, 768
XapI RAATTY 1 cut(s) 18
XceI RCATGY 3 cut(s) 163, 808, 1748
XcmI CCANNNNNNNNNTGG 2 cut(s) 580, 1002
XmiI GTMKAC 1 cut(s) 728
XspI CTAG 1 cut(s) 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.