AT1G68170

Nodulin MtN21 EamA-like transporter family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000337
Physical Location & Seq
Forward (+)
25551490 .. 25554471
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68170.1

Sequence Viewer

Length: 1071 bp
ATGAAAGATATAACGGCAATGGTGGTGGTTCAAATAGCTACGGCAGGGTTAAACATATTTTTCAAGCTAGCAATGGAAGATGGTATGAACCCAAGTGTACTCGTGGCCTATCGTCTCTTGTTTGCTACACTTTTCATGATTCCTATATGCTTCATCTTTCAAAGGAAGAAGAGGCCAGAGTTTACATGCAGGCTGATGTTACTAGCATTACTCTCAGGGTTACTCGGTGTGGTGATACCAAGTATCCTAACCATTACGGGTCTAGCTCTAACGTCAGCAACATTCACCTCTGCTGCTGGCGTTCTCACTCCATTGGTCACTTTCATCTTCGCTGCACTTCTTAGGATGGAGAGTGTACGGCTTGGATCGAGCGTAGGGCTGGCTAAGGTTTTTGGAACATTGTTTGGTGTTGGTGGAGCTCTTGTCTTTATATTCTATAGAGGAATAGAGATCCGACTATGGTCAACTCACGTTAACCTAGTGAACCAACCACGTGACTCCTCACGTGATGCTACCACTCACCATATTTCCATCCTTGGGGCTCTTCTAGTTTTTGGGGGAAACATTTCCATCTCACTTTGGTTTTTATTGCAGGTTAAGATAAGCAAACAATTTGGAGGGCCTTATTGGAACGCGACTCTAATGAACATGATGGGAGGCGTAGTGGCTATGCTTGTCGCTCTTTGCTGGGAACACGATTTAGATGAATGGAGATTAGGATGGAATATCAGACTATTAACCATTGCTTATGCGGCAATTCTGATTTCCGGGATGGTGGTAGCAGTCAACGCATGGTGCATTGAGTCTAGAGGACCGTTATTTGTATCGGTATTTAGCCCTGTAGGACTTGTGATCGTAGCTCTTGTTGGATCATTTTTGCTAGATGAAACACTTCATTTGGGGAGCATAATTGGTACAGTGATTATCGTTGGAGCATTATACATTGTGCTATGGGCTAAAAACAAGGAAATGAAGAGTATGCTTACGACATCGGATCATAATGAAACAAATAAGACTAGCAAAGATATCACAGTCAACAATCTCCCAACATTAAGTACAAATGTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

356

Amino Acids

38.8

Weight (kDa)

9.23

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 5 - 142 9.3e-13 EamA-like transporter family
EamA PF00892 179 - 317 9.7e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 583
AccII CGCG 1 cut(s) 635
AciI CCGC 1 cut(s) 752
AclWI GGATC 4 cut(s) 373, 445, 877, 1002
AcvI CACGTG 2 cut(s) 494, 506
AfaI GTAC 4 cut(s) 99, 357, 916, 1057
AfiI CCNNNNNNNGG 1 cut(s) 537
AgsI TTSAA 3 cut(s) 32, 64, 161
AluBI AGCT 5 cut(s) 38, 67, 266, 419, 860
AluI AGCT 5 cut(s) 38, 67, 266, 419, 860
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 4 cut(s) 373, 445, 877, 1002
AoxI GGCC 3 cut(s) 105, 173, 620
ApeKI GCWGC 2 cut(s) 293, 332
Asp700I GAANNNNTTC 2 cut(s) 565, 891
AspS9I GGNCC 2 cut(s) 620, 812
AsuC2I CCSGG 1 cut(s) 769
AsuHPI GGTGA 3 cut(s) 244, 277, 512
AsuNHI GCTAGC 1 cut(s) 67
AvaII GGWCC 1 cut(s) 812
BanII GRGCYC 2 cut(s) 421, 544
BauI CACGAG 1 cut(s) 101
BbrPI CACGTG 2 cut(s) 494, 506
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 280, 319
BccI CCATC 7 cut(s) 74, 340, 539, 578, 646, 714, 766
BceAI ACGGC 3 cut(s) 30, 57, 374
BciVI GTATCC 1 cut(s) 254
BcnI CCSGG 1 cut(s) 769
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 8 cut(s) 68, 203, 263, 479, 548, 807, 881, 1017
BfmI CTRYAG 2 cut(s) 436, 840
BfuAI ACCTGC 1 cut(s) 583
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 3 cut(s) 294, 333, 753
BlsI GCNGC 3 cut(s) 295, 334, 754
Bme1390I CCNGG 1 cut(s) 769
Bme18I GGWCC 1 cut(s) 812
BmgT120I GGNCC 2 cut(s) 620, 812
BmrFI CCNGG 1 cut(s) 769
BmsI GCATC 1 cut(s) 499
BmtI GCTAGC 1 cut(s) 71
BoxI GACNNNNGTC 1 cut(s) 460
Bpu10I CCTNAGC 1 cut(s) 384
BpuMI CCSGG 1 cut(s) 769
BsaAI YACGTR 2 cut(s) 494, 506
BsaJI CCNNGG 1 cut(s) 535
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 648, 678
Bsc4I CCNNNNNNNGG 1 cut(s) 537
Bse3DI GCAATG 3 cut(s) 24, 78, 741
BseDI CCNNGG 1 cut(s) 535
BseGI GGATG 4 cut(s) 351, 531, 725, 777
BseLI CCNNNNNNNGG 1 cut(s) 537
BseMI GCAATG 3 cut(s) 24, 78, 741
BseMII CTCAG 1 cut(s) 228
BseRI GAGGAG 1 cut(s) 490
BseXI GCAGC 2 cut(s) 280, 319
BseYI CCCAGC 1 cut(s) 687
BsgI GTGCAG 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 635
BshFI GGCC 3 cut(s) 107, 175, 622
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 1 cut(s) 768
BslFI GGGAC 1 cut(s) 1049
BslI CCNNNNNNNGG 1 cut(s) 537
BsmAI GTCTC 1 cut(s) 119
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 1049
BsnI GGCC 3 cut(s) 107, 175, 622
Bsp1286I GDGCHC 2 cut(s) 421, 544
Bsp143I GATC 5 cut(s) 365, 450, 852, 869, 994
BspACI CCGC 1 cut(s) 752
BspANI GGCC 3 cut(s) 107, 175, 622
BspCNI CTCAG 1 cut(s) 227
BspFNI CGCG 1 cut(s) 635
BspHI TCATGA 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 583
BspOI GCTAGC 1 cut(s) 71
BspPI GGATC 4 cut(s) 373, 445, 877, 1002
BspQI GCTCTTC 1 cut(s) 549
BsrDI GCAATG 3 cut(s) 24, 78, 741
BssECI CCNNGG 1 cut(s) 535
BssMI GATC 5 cut(s) 365, 450, 852, 869, 994
BssSI CACGAG 1 cut(s) 101
BssT1I CCWWGG 1 cut(s) 535
Bst2BI CACGAG 1 cut(s) 101
Bst4CI ACNGT 3 cut(s) 816, 919, 1033
Bst6I CTCTTC 3 cut(s) 164, 549, 968
BstBAI YACGTR 2 cut(s) 494, 506
BstC8I GCNNGC 4 cut(s) 69, 191, 298, 381
BstDEI CTNAG 3 cut(s) 214, 341, 384
BstF5I GGATG 4 cut(s) 351, 531, 725, 777
BstFNI CGCG 1 cut(s) 635
BstKTI GATC 5 cut(s) 368, 453, 855, 872, 997
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 5 cut(s) 365, 450, 852, 869, 994
BstMWI GCNNNNNNNGC 2 cut(s) 752, 788
BstNSI RCATGY 1 cut(s) 189
BstPAI GACNNNNGTC 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 767
BstSFI CTRYAG 2 cut(s) 436, 840
BstUI CGCG 1 cut(s) 635
BstV1I GCAGC 2 cut(s) 280, 319
BstX2I RGATCY 1 cut(s) 450
BstYI RGATCY 1 cut(s) 450
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 3 cut(s) 107, 175, 622
BtsCI GGATG 4 cut(s) 351, 531, 725, 777
BtsIMutI CAGTG 1 cut(s) 924
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 4 cut(s) 69, 191, 298, 381
CciI TCATGA 1 cut(s) 135
Cfr13I GGNCC 2 cut(s) 620, 812
Csp6I GTAC 4 cut(s) 98, 356, 915, 1056
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 4 cut(s) 136, 186, 649, 792
CviQI GTAC 4 cut(s) 98, 356, 915, 1056
DdeI CTNAG 3 cut(s) 214, 341, 384
DpnI GATC 5 cut(s) 367, 452, 854, 871, 996
DpnII GATC 5 cut(s) 365, 450, 852, 869, 994
Eam1104I CTCTTC 3 cut(s) 164, 549, 968
EarI CTCTTC 3 cut(s) 164, 549, 968
Ecl136II GAGCTC 1 cut(s) 419
Eco130I CCWWGG 1 cut(s) 535
Eco24I GRGCYC 2 cut(s) 421, 544
Eco32I GATATC 1 cut(s) 1027
Eco47I GGWCC 1 cut(s) 812
Eco53kI GAGCTC 1 cut(s) 419
Eco72I CACGTG 2 cut(s) 494, 506
EcoICRI GAGCTC 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 620
EcoRV GATATC 1 cut(s) 1027
EcoT14I CCWWGG 1 cut(s) 535
EcoT38I GRGCYC 2 cut(s) 421, 544
ErhI CCWWGG 1 cut(s) 535
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 4 cut(s) 139, 189, 652, 795
FaqI GGGAC 1 cut(s) 1049
FatI CATG 4 cut(s) 135, 185, 648, 791
Fnu4HI GCNGC 3 cut(s) 294, 333, 753
FokI GGATG 4 cut(s) 358, 518, 732, 784
FriOI GRGCYC 2 cut(s) 421, 544
Fsp4HI GCNGC 3 cut(s) 294, 333, 753
FspBI CTAG 8 cut(s) 68, 203, 263, 479, 548, 807, 881, 1017
GluI GCNGC 3 cut(s) 294, 333, 753
GsaI CCCAGC 1 cut(s) 691
HaeIII GGCC 3 cut(s) 107, 175, 622
HapII CCGG 1 cut(s) 768
Hin1II CATG 4 cut(s) 139, 189, 652, 795
HincII GTYRAC 4 cut(s) 465, 475, 787, 1036
HindII GTYRAC 4 cut(s) 465, 475, 787, 1036
HinfI GANTC 4 cut(s) 139, 497, 637, 803
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 1 cut(s) 768
HphI GGTGA 3 cut(s) 244, 277, 512
Hpy166II GTNNAC 8 cut(s) 98, 183, 356, 465, 475, 484, 787, 1036
Hpy188I TCNGA 4 cut(s) 455, 731, 762, 994
Hpy188III TCNNGA 2 cut(s) 136, 807
Hpy8I GTNNAC 8 cut(s) 98, 183, 356, 465, 475, 484, 787, 1036
HpyCH4III ACNGT 3 cut(s) 816, 919, 1033
HpyCH4IV ACGT 4 cut(s) 272, 471, 493, 505
HpyCH4V TGCA 4 cut(s) 189, 335, 592, 798
HpyF10VI GCNNNNNNNGC 2 cut(s) 752, 788
HpyF3I CTNAG 3 cut(s) 214, 341, 384
HpySE526I ACGT 4 cut(s) 272, 471, 493, 505
Hsp92II CATG 4 cut(s) 139, 189, 652, 795
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 5 cut(s) 365, 450, 852, 869, 994
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 3 cut(s) 416, 903, 932
Lsp1109I GCAGC 2 cut(s) 280, 319
LweI GCATC 1 cut(s) 499
MaeI CTAG 8 cut(s) 68, 203, 263, 479, 548, 807, 881, 1017
MaeII ACGT 4 cut(s) 272, 471, 493, 505
MaeIII GTNAC 4 cut(s) 198, 219, 316, 494
MalI GATC 5 cut(s) 367, 452, 854, 871, 996
MboI GATC 5 cut(s) 365, 450, 852, 869, 994
MboII GAAGA 6 cut(s) 89, 178, 181, 319, 536, 985
MflI RGATCY 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 421, 544
MluCI AATT 3 cut(s) 611, 756, 909
MlyI GAGTC 3 cut(s) 491, 631, 812
MmeI TCCRAC 3 cut(s) 478, 847, 910
MnlI CCTC 7 cut(s) 165, 298, 434, 511, 611, 650, 803
MroXI GAANNNNTTC 2 cut(s) 565, 891
MseI TTAA 5 cut(s) 50, 474, 597, 737, 1052
MspI CCGG 1 cut(s) 768
MspR9I CCNGG 1 cut(s) 769
MvnI CGCG 1 cut(s) 635
MwoI GCNNNNNNNGC 2 cut(s) 752, 788
NciI CCSGG 1 cut(s) 769
NdeII GATC 5 cut(s) 365, 450, 852, 869, 994
NheI GCTAGC 1 cut(s) 67
NlaIII CATG 4 cut(s) 139, 189, 652, 795
NmuCI GTSAC 2 cut(s) 316, 494
NspI RCATGY 1 cut(s) 189
PagI TCATGA 1 cut(s) 135
PciSI GCTCTTC 1 cut(s) 549
PdmI GAANNNNTTC 2 cut(s) 565, 891
PfeI GAWTC 1 cut(s) 139
PfoI TCCNGGA 1 cut(s) 767
PkrI GCNGC 3 cut(s) 295, 334, 754
PleI GAGTC 3 cut(s) 491, 631, 811
PmaCI CACGTG 2 cut(s) 494, 506
PmlI CACGTG 2 cut(s) 494, 506
PpsI GAGTC 3 cut(s) 491, 631, 811
Ppu21I YACGTR 2 cut(s) 494, 506
PshAI GACNNNNGTC 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 421
PspCI CACGTG 2 cut(s) 494, 506
PspFI CCCAGC 1 cut(s) 687
PspPI GGNCC 2 cut(s) 620, 812
PsuI RGATCY 1 cut(s) 450
RsaI GTAC 4 cut(s) 99, 357, 916, 1057
RsaNI GTAC 4 cut(s) 98, 356, 915, 1056
SacI GAGCTC 1 cut(s) 421
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 5 cut(s) 50, 474, 597, 737, 1052
SatI GCNGC 3 cut(s) 294, 333, 753
Sau3AI GATC 5 cut(s) 365, 450, 852, 869, 994
Sau96I GGNCC 2 cut(s) 620, 812
SchI GAGTC 3 cut(s) 491, 631, 812
ScrFI CCNGG 1 cut(s) 769
SduI GDGCHC 2 cut(s) 421, 544
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 2 cut(s) 436, 840
SinI GGWCC 1 cut(s) 812
Sse9I AATT 3 cut(s) 611, 756, 909
SsiI CCGC 1 cut(s) 752
SspMI CTAG 8 cut(s) 68, 203, 263, 479, 548, 807, 881, 1017
SstI GAGCTC 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 767
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 3 cut(s) 816, 919, 1033
TaiI ACGT 4 cut(s) 275, 474, 496, 508
TaqI TCGA 1 cut(s) 368
TasI AATT 3 cut(s) 611, 756, 909
TatI WGTACW 2 cut(s) 97, 1055
TauI GCSGC 1 cut(s) 755
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 5 cut(s) 50, 474, 597, 737, 1052
Tru9I TTAA 5 cut(s) 50, 474, 597, 737, 1052
TscAI CASTG 1 cut(s) 924
TseFI GTSAC 2 cut(s) 316, 494
TseI GCWGC 2 cut(s) 293, 332
Tsp45I GTSAC 2 cut(s) 316, 494
TspRI CASTG 1 cut(s) 924
VpaK11BI GGWCC 1 cut(s) 812
XbaI TCTAGA 1 cut(s) 806
XceI RCATGY 1 cut(s) 189
XmnI GAANNNNTTC 2 cut(s) 565, 891
XspI CTAG 8 cut(s) 68, 203, 263, 479, 548, 807, 881, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.