RchiOBHm_Chr7g0224641

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000337
Physical Location & Seq
Forward (+)
46927185 .. 46929002
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20114

Sequence Viewer

Length: 1125 bp
ATGGGGAAGCAGAGTAGTGAGAGTAGTGTAGTTGATGGCTTAATGAAGCCAGTGATAACGATGGTGATCGCTCAGATTGCATATGCAGCAACGAGTATATTCTACAAGGTTGCAGCAGACTCTGGAATGAATCTCAGGATTCTCGTTGCCTACCGTCTCATGTTTTCATCTGCCATTATCGTTCCTCTTGCTCTTATACTCGAACGGGGATCATTGTCAGAAAATTTATTCATAGAGGGATTAGTCTTAACATCACCAACCTTTGTTGCAGCCACTGCAAATCTGATTCCAGCCTTCACCTTCATCATGGCCATCTCTTTCAGGTTGGAGAAGTTGGCACTAGGAAGCCATGCAGGGAAGGCAAAGCTTGTGGGAACAGCGGTGGGTCTTGGTGGTGCTATGATCTTTACCTTCTTCAAAGGACCAGAGTTCTCCATCTGGTCAACTCACGTTGACCTTCTTCGAGGACATCAGGCGGCAACATCACCGTTATCGTCCTCCTCCCATAGAAGCACCGGGAGTCACCTGCTGGGCTCCTTCTTGGCACTCGGCTGCGTCGTTTCCTATGCAATGTGGCTCATAGTTCAGGCAAAAATGAGCAAGAGATATCCATGCCATTACTCGAGCACGGCTCTATTGTCGGTCATGGGATCGATTCAGTCTGTAGCTTTTGCTCTCTGCGTGGAGAGGGATTGGAGCCAGTGGAAATTGGGTTGGAACATCAAGCTTTTTACAGCTGCATATGGGGGAATTGTGACTTCTGGGATGGTGGTGGTTTTGATCTCATGGTGTGTACGCAAGCGAGGTCCATTGTTTGTGTCAGTTTTCACTCCGCTTTCCCTAGTGATTGTGGCCATTGCTAGTTCTTTATTGCTCAATGAAAAGTTAAGCCTAGGCAGCGTATTTGGAGGGATTCTAATTGTATGCGGGTTGTACATGGTGCTCTGGGGGCAGAGCAAAGAGATGAAGACGATTAATAATGTGACTGATGATCAAGATCGACAACATCCAGCTGCAGCAAGGCTCCAAGATTCCCAAATCATCCAAGTCGTCGCAACGACTCGATCAGTTGACACTAATACCTCGTGTGAGAACATCAACACCACAAATGTTGGGTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

374

Amino Acids

40.2

Weight (kDa)

9.32

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 177 - 315 3e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 534
Acc36I ACCTGC 1 cut(s) 534
AciI CCGC 4 cut(s) 380, 476, 833, 927
AclWI GGATC 2 cut(s) 217, 658
AcoI YGGCCR 2 cut(s) 309, 852
AcsI RAATTY 1 cut(s) 223
AfaI GTAC 2 cut(s) 795, 935
AgsI TTSAA 1 cut(s) 418
AluBI AGCT 5 cut(s) 367, 668, 727, 737, 1013
AluI AGCT 5 cut(s) 367, 668, 727, 737, 1013
Alw21I GWGCWC 2 cut(s) 629, 945
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 2 cut(s) 217, 658
AlwNI CAGNNNCTG 2 cut(s) 122, 275
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 2 cut(s) 309, 852
ApeKI GCWGC 8 cut(s) 86, 113, 269, 552, 737, 897, 1013, 1016
ApoI RAATTY 1 cut(s) 223
ArsI GACNNNNNNTTYG 1 cut(s) 1100
AseI ATTAAT 1 cut(s) 975
AspA2I CCTAGG 1 cut(s) 892
AspS9I GGNCC 2 cut(s) 422, 806
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 5 cut(s) 76, 246, 289, 477, 515
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 2 cut(s) 422, 806
AvrII CCTAGG 1 cut(s) 892
BalI TGGCCA 2 cut(s) 311, 854
BanII GRGCYC 1 cut(s) 536
BauI CACGAG 1 cut(s) 1084
BbsI GAAGAC 1 cut(s) 974
Bbv12I GWGCWC 2 cut(s) 629, 945
BbvI GCAGC 8 cut(s) 98, 125, 281, 539, 724, 909, 1000, 1028
BccI CCATC 5 cut(s) 29, 55, 320, 443, 760
BceAI ACGGC 1 cut(s) 645
BclI TGATCA 1 cut(s) 991
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 4 cut(s) 341, 842, 861, 893
BfmI CTRYAG 2 cut(s) 663, 1014
BfuAI ACCTGC 1 cut(s) 534
BisI GCNGC 9 cut(s) 87, 114, 270, 477, 553, 738, 898, 1014, 1017
BlnI CCTAGG 1 cut(s) 892
BlsI GCNGC 9 cut(s) 88, 115, 271, 478, 554, 739, 899, 1015, 1018
Bme1390I CCNGG 1 cut(s) 517
Bme18I GGWCC 2 cut(s) 422, 806
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 422, 806
BmiI GGNNCC 3 cut(s) 535, 698, 1025
BmrFI CCNGG 1 cut(s) 517
BpiI GAAGAC 1 cut(s) 974
BplI GAGNNNNNCTC 2 cut(s) 616, 648
BpuMI CCSGG 1 cut(s) 517
Bsa29I ATCGAT 1 cut(s) 653
BsaBI GATNNNNATC 2 cut(s) 65, 996
BsaJI CCNNGG 1 cut(s) 892
Bse1I ACTGG 2 cut(s) 50, 700
Bse3DI GCAATG 2 cut(s) 576, 855
Bse8I GATNNNNATC 2 cut(s) 65, 996
BseCI ATCGAT 1 cut(s) 653
BseDI CCNNGG 1 cut(s) 892
BseGI GGATG 3 cut(s) 771, 1006, 1041
BseJI GATNNNNATC 2 cut(s) 65, 996
BseMI GCAATG 2 cut(s) 576, 855
BseMII CTCAG 2 cut(s) 86, 148
BseNI ACTGG 2 cut(s) 50, 700
BseRI GAGGAG 1 cut(s) 490
BseXI GCAGC 8 cut(s) 98, 125, 281, 539, 724, 909, 1000, 1028
BseYI CCCAGC 1 cut(s) 529
BshFI GGCC 2 cut(s) 311, 854
BshVI ATCGAT 1 cut(s) 653
BsiHKAI GWGCWC 2 cut(s) 629, 945
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 1 cut(s) 516
BsmAI GTCTC 1 cut(s) 161
BsmBI CGTCTC 1 cut(s) 161
BsnI GGCC 2 cut(s) 311, 854
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 3 cut(s) 536, 629, 945
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
BspACI CCGC 4 cut(s) 380, 476, 833, 927
BspANI GGCC 2 cut(s) 311, 854
BspCNI CTCAG 2 cut(s) 85, 147
BspDI ATCGAT 1 cut(s) 653
BspLI GGNNCC 3 cut(s) 535, 698, 1025
BspMAI CTGCAG 1 cut(s) 1018
BspMI ACCTGC 1 cut(s) 534
BspPI GGATC 2 cut(s) 217, 658
BsrDI GCAATG 2 cut(s) 576, 855
BsrGI TGTACA 1 cut(s) 933
BsrI ACTGG 2 cut(s) 50, 700
BssECI CCNNGG 1 cut(s) 892
BssMI GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
BssSI CACGAG 1 cut(s) 1084
BssT1I CCWWGG 1 cut(s) 892
Bst2BI CACGAG 1 cut(s) 1084
Bst4CI ACNGT 2 cut(s) 155, 489
BstAPI GCANNNNNTGC 1 cut(s) 275
BstAUI TGTACA 1 cut(s) 933
BstC8I GCNNGC 1 cut(s) 800
BstDEI CTNAG 2 cut(s) 72, 134
BstF5I GGATG 3 cut(s) 771, 1006, 1041
BstKTI GATC 8 cut(s) 69, 212, 405, 653, 783, 994, 1000, 1067
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
BstMWI GCNNNNNNNGC 6 cut(s) 77, 86, 275, 359, 897, 949
BstSCI CCNGG 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 663, 1014
BstV1I GCAGC 8 cut(s) 98, 125, 281, 539, 724, 909, 1000, 1028
BstV2I GAAGAC 1 cut(s) 974
Bsu15I ATCGAT 1 cut(s) 653
BsuRI GGCC 2 cut(s) 311, 854
BsuTUI ATCGAT 1 cut(s) 653
BtsCI GGATG 3 cut(s) 771, 1006, 1041
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 57, 273, 707
BveI ACCTGC 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 800
CaiI CAGNNNCTG 2 cut(s) 122, 275
Cfr13I GGNCC 2 cut(s) 422, 806
ClaI ATCGAT 1 cut(s) 653
CseI GACGC 1 cut(s) 544
Csp6I GTAC 2 cut(s) 794, 934
CspCI CAANNNNNGTGG 3 cut(s) 351, 386, 1093
CviAII CATG 7 cut(s) 160, 307, 350, 612, 646, 786, 937
CviQI GTAC 2 cut(s) 794, 934
DdeI CTNAG 2 cut(s) 72, 134
DpnI GATC 8 cut(s) 68, 211, 404, 652, 782, 993, 999, 1066
DpnII GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
EaeI YGGCCR 2 cut(s) 309, 852
Eco130I CCWWGG 1 cut(s) 892
Eco24I GRGCYC 1 cut(s) 536
Eco32I GATATC 1 cut(s) 608
Eco47I GGWCC 2 cut(s) 422, 806
Eco88I CYCGRG 1 cut(s) 622
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 892
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 7 cut(s) 163, 310, 353, 615, 649, 789, 940
FatI CATG 7 cut(s) 159, 306, 349, 611, 645, 785, 936
FauI CCCGC 1 cut(s) 920
FauNDI CATATG 2 cut(s) 82, 742
FbaI TGATCA 1 cut(s) 991
Fnu4HI GCNGC 9 cut(s) 87, 114, 270, 477, 553, 738, 898, 1014, 1017
FokI GGATG 3 cut(s) 778, 993, 1028
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 9 cut(s) 87, 114, 270, 477, 553, 738, 898, 1014, 1017
FspBI CTAG 4 cut(s) 341, 842, 861, 893
GluI GCNGC 9 cut(s) 87, 114, 270, 477, 553, 738, 898, 1014, 1017
GsaI CCCAGC 1 cut(s) 533
HaeIII GGCC 2 cut(s) 311, 854
HapII CCGG 1 cut(s) 516
HgaI GACGC 1 cut(s) 544
Hin1II CATG 7 cut(s) 163, 310, 353, 615, 649, 789, 940
HincII GTYRAC 3 cut(s) 444, 454, 1072
HindII GTYRAC 3 cut(s) 444, 454, 1072
HindIII AAGCTT 2 cut(s) 365, 725
HinfI GANTC 9 cut(s) 119, 130, 139, 286, 520, 655, 913, 1031, 1060
HpaII CCGG 1 cut(s) 516
HphI GGTGA 5 cut(s) 76, 246, 289, 477, 515
Hpy166II GTNNAC 4 cut(s) 444, 454, 794, 1072
Hpy188I TCNGA 3 cut(s) 75, 220, 285
Hpy188III TCNNGA 3 cut(s) 123, 136, 995
Hpy8I GTNNAC 4 cut(s) 444, 454, 794, 1072
Hpy99I CGWCG 2 cut(s) 560, 1055
HpyAV CCTTC 6 cut(s) 304, 310, 352, 421, 467, 547
HpyCH4III ACNGT 2 cut(s) 155, 489
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 9 cut(s) 80, 86, 113, 269, 278, 353, 569, 740, 1016
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 86, 275, 359, 897, 949
HpyF3I CTNAG 2 cut(s) 72, 134
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 7 cut(s) 163, 310, 353, 615, 649, 789, 940
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
LmnI GCTCC 3 cut(s) 539, 696, 1029
Lsp1109I GCAGC 8 cut(s) 98, 125, 281, 539, 724, 909, 1000, 1028
MaeI CTAG 4 cut(s) 341, 842, 861, 893
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 3 cut(s) 521, 754, 982
MalI GATC 8 cut(s) 68, 211, 404, 652, 782, 993, 999, 1066
MboI GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
MboII GAAGA 3 cut(s) 406, 452, 979
MhlI GDGCHC 3 cut(s) 536, 629, 945
MlsI TGGCCA 2 cut(s) 311, 854
MluCI AATT 4 cut(s) 223, 707, 750, 918
MluNI TGGCCA 2 cut(s) 311, 854
MlyI GAGTC 3 cut(s) 113, 529, 1054
MmeI TCCRAC 2 cut(s) 306, 695
MnlI CCTC 9 cut(s) 195, 229, 458, 508, 511, 681, 797, 902, 1093
Mox20I TGGCCA 2 cut(s) 311, 854
MscI TGGCCA 2 cut(s) 311, 854
MseI TTAA 4 cut(s) 41, 248, 887, 975
Msp20I TGGCCA 2 cut(s) 311, 854
MspA1I CMGCKG 3 cut(s) 380, 737, 1013
MspI CCGG 1 cut(s) 516
MspR9I CCNGG 1 cut(s) 517
MwoI GCNNNNNNNGC 6 cut(s) 77, 86, 275, 359, 897, 949
NciI CCSGG 1 cut(s) 517
NdeI CATATG 2 cut(s) 82, 742
NdeII GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
NlaIII CATG 7 cut(s) 163, 310, 353, 615, 649, 789, 940
NlaIV GGNNCC 3 cut(s) 535, 698, 1025
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 3 cut(s) 521, 754, 982
PaeR7I CTCGAG 1 cut(s) 622
PaqCI CACCTGC 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PfeI GAWTC 6 cut(s) 130, 139, 286, 655, 913, 1031
PkrI GCNGC 9 cut(s) 88, 115, 271, 478, 554, 739, 899, 1015, 1018
PleI GAGTC 3 cut(s) 113, 528, 1054
PpsI GAGTC 3 cut(s) 113, 528, 1054
PshBI ATTAAT 1 cut(s) 975
PspFI CCCAGC 1 cut(s) 529
PspN4I GGNNCC 3 cut(s) 535, 698, 1025
PspPI GGNCC 2 cut(s) 422, 806
PspXI VCTCGAGB 1 cut(s) 622
PstI CTGCAG 1 cut(s) 1018
PstNI CAGNNNCTG 2 cut(s) 122, 275
PvuII CAGCTG 2 cut(s) 737, 1013
RsaI GTAC 2 cut(s) 795, 935
RsaNI GTAC 2 cut(s) 794, 934
SaqAI TTAA 4 cut(s) 41, 248, 887, 975
SatI GCNGC 9 cut(s) 87, 114, 270, 477, 553, 738, 898, 1014, 1017
Sau3AI GATC 8 cut(s) 66, 209, 402, 650, 780, 991, 997, 1064
Sau96I GGNCC 2 cut(s) 422, 806
SchI GAGTC 3 cut(s) 113, 529, 1054
ScrFI CCNGG 1 cut(s) 517
SduI GDGCHC 3 cut(s) 536, 629, 945
SfcI CTRYAG 2 cut(s) 663, 1014
Sfr274I CTCGAG 1 cut(s) 622
SinI GGWCC 2 cut(s) 422, 806
SlaI CTCGAG 1 cut(s) 622
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
Sse9I AATT 4 cut(s) 223, 707, 750, 918
SsiI CCGC 4 cut(s) 380, 476, 833, 927
SspMI CTAG 4 cut(s) 341, 842, 861, 893
StyD4I CCNGG 1 cut(s) 515
StyI CCWWGG 1 cut(s) 892
TaaI ACNGT 2 cut(s) 155, 489
TaiI ACGT 1 cut(s) 453
TaqI TCGA 6 cut(s) 201, 463, 623, 653, 1000, 1063
TaqII GACCGA 1 cut(s) 631
TasI AATT 4 cut(s) 223, 707, 750, 918
TatI WGTACW 1 cut(s) 933
TauI GCSGC 1 cut(s) 479
TfiI GAWTC 6 cut(s) 130, 139, 286, 655, 913, 1031
Tru1I TTAA 4 cut(s) 41, 248, 887, 975
Tru9I TTAA 4 cut(s) 41, 248, 887, 975
TscAI CASTG 3 cut(s) 57, 280, 707
TseFI GTSAC 3 cut(s) 521, 754, 982
TseI GCWGC 8 cut(s) 86, 113, 269, 552, 737, 897, 1013, 1016
Tsp45I GTSAC 3 cut(s) 521, 754, 982
TspDTI ATGAA 7 cut(s) 59, 143, 156, 220, 292, 894, 980
TspRI CASTG 3 cut(s) 57, 280, 707
VpaK11BI GGWCC 2 cut(s) 422, 806
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 622
XmaJI CCTAGG 1 cut(s) 892
XspI CTAG 4 cut(s) 341, 842, 861, 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.