Rorug07G0224100

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000337
Physical Location & Seq
Reverse (-)
20399431 .. 20411326
11896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0224100.1

Sequence Viewer

Length: 3831 bp
ATGGAATCGCAGCCGAACAACCTCTTCGACACGGCGTCGCAGCCGGACACGGCCACCGACGCCTACACTTTCCTCGAATTCAACACCCAGGGCGAGGACTTCGACTACCCGGAGTTCCGCGACCCGATTCGGTCCCCGGTGGCCTGGCCCACGCCGTCCGATTCCTTATCGGACCCTGCCGACCGCGACCGCGGCGGAGGAGGAGGTGGTGGTGGCTCCGACCACCAGTCTGATGCGTCGCCTGTTTCGGCTGCCCCGGGCAGCGCCACGAAGGCTCGGGCCGGTGGCTCGGGAAGTAGCGGCGGGAATAATAATCAGGTGGTTGACGCCCTAGCGTCTGGGATGAGTGTGCTGAACTTTGAGGACACTGGGGACGATGATAGCTATGAGTTTGGAAAGGGGGATTTCACTGAGCATGCGTGCCGGTACTGCGGCGTGTCGAACCCGGCGTGTGTTGTGAGGTGTAACGTGCCGTCGTGCCGGAAGTGGTTTTGTAATTCCAGGGGGAACACGTCCGGCTCTCATATTGTGAATCACCTGGTTCGTGCAAAACACAAGGAAGTCTGTCTTCATAAAGATAGCCCATTAGGAGAAACAATCCTTGAGTGCTACAACTGTGGATGCCGCAATGTATTCCTTCTTGGATTTATTTCAGCCAAGACAGAGAGTGTTGTTGTTCTTCTCTGTAGGGAACCCTGCTTAAGTGTTAATGCATTGAAGGATATGAACTGGGACCTGAGCCAGTGGTGTCCCCTGATTGATGATAGGTGTTTCTTGCAGTGGCTTGTTAAGGTCCCCTCCGAACAGGAGCAGCTGAGGGCACGCCAGATTAGTGCTCAACAAATAAACAAGGTAGAAGAACTCTGGAAGTCAAATCCTGATGCTTCCCTTGAAGATCTTGAGAAGCCTGGTGTGGATGATGAACCCCAACCTGTGGCCGTAAAGTATGAGGATGCATACCAGTATCAAAATGTGTTTGCACCACTTATCAAGCTTGAAGCTGACTATGATAAAATGATGAAAGAATCTCAAAGCAAGGACAATCTCACCATTAGGTGGGATATTGGTCTTAACAAGAAGCGTGTCGCTTATTTTATCTTTCCAAAGGAGGACAATGAGATTCGTCTTGTACCTGGTGATGAGCTGCGGCTTCGTTATTCTGGGGATGCAGCTCATCCAGCATGGCAGTCTGTGGGACATGTGATCAAGTTAACTGCACAAGAGGAGGTTGCGCTCGAACTTCGTGCTAGTCAGGGAGTTCCAGCTGATGTGAACCATGGCTTCAGCGTTGATTTCGTGTGGAAGAGTACAAGTTTTGATCGTATGCAGGGAGCCATGAAAGCTTTTGCAGTGGATGAGACAAGTGTTAGTGGGTATATTTACCATCACTTGTTAGGCCATGAAGTAGAGGTTCAGATGGTCCGTAATACACTGCCTCGTCGTTTTGGTGCACCCGGTCTGCCAGAGCTCAATGCATCTCAGGTTTTTGCTGTGAAAAGTGTTCTTCAGAAGCCTATAAGTTTGATTCAAGGTCCACCTGGCACAGGAAAAACCGTCACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGGCAGGGACAGGTCTTGGTTTGCGCTCCTAGTAATGTGGCTGTAGACCAACTAGCTGAAAAAATAAGTGCAACTGGGCTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCTGTCAGCTCTCCTGTTGAGCATTTAACCCTTCACTATCAGGTTCGTCATCTTGACACATCAGAAAAGAGTGAACTTCACAAGTTACAGCAATTGAAGGATGAGCAAGGCGAATTGTCAAGTAGTGATGAGAAAAAGTACAAAGCGCTGAAGCGGGCAACTGAGAGAGAGATTTCTCAGAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGAGATCCTCGATTGGCGAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCCACTCAGGCAACAGAGCCTGAATGCCTTATTCCTTTGGTACTAGGGGCAAAGCAGGTTGTTTTGGTCGGTGATCACTGCCAGCTTGGTCCAGTCATTATGTGCAAGAAAGCAGCTCGTGCTGGGCTTGCCCAGTCTCTTTTTGAACGCCTCGTTTTACTTGGTGTGAAACCAATTAGGTTGCAGGTCCAATACAGAATGCATCCAGCCTTGTCAGAATTTCCTTCCAACAGCTTCTATGAGGGAACACTCCAAAATGGCGTCACCATCAATGAAAGGCAATCAACGGGAATTGATTTTCCGTGGCCAGTTCCCAATCGTCCCATGTTCTTTTATGTTCAGATGGGGCAAGAGGAGATAAGTGCTAGCGGAACATCTTACCTGAACAGAACTGAAGCTGCTAATGTTGAAAAGATTGTCACAACATTCTTAAGGAGTGGTGTCGTACCTAGTCAGATCGGGGTGATAACACCATATGAGGGACAGCGAGCATATATTGTGAACTATATGTCAAGAAATGGTGCTCTCAGGCAGCAACTTTATAAGGAAATAGAGGTCGCAAGTGTAGATTCATTTCAAGGAAGGGAAAAAGATTATATCATCCTTTCATGTGTGAGGAGTAATGAGCATCAGGGCATTGGATTCCTTAACGACCCTCGGAGGCTCAATGTTGCCCTAACACGTGCTCGGTATGGTATTGTTATTCTGGGGAACCCTAAAGTTCTAAGTAAACAGCCCTTGTGGAATAGCTTGTTGACGCACTACAAGGAACATGAGTGCTTGGTCGAAGGTCCTCTAAATAACTTGAAGCAGAGTATGGTTCAATTTCCAAAGCCCAAAAAGATCTACAATGATCGGCGTCTGTTCTTTGGTGGTGGTCCTGGAGTTGTCCCAAGTGATAGTTATGGGTCTATTCCCCCATCCGGTCAGAGTGCTGATAGAAGAAGTGGTCGTGGTAGGGGCTCTTACCTACCTCCTGGTCCACCGAATGGTACCCACAAGCCTGGGGTACATCCTGCTGGATATCCAATGCCTCGGGCACCACTTGCACCATTTCATGGTGGTCCCCTTTCTCAACCCTATGCTATTCCAACTCGTGGAGCCGTGCATGGACCAGTAGGAGCTGTTCCTCATGTCCCTCAACCTGGAAGTCGAGGTTTTGGAGCTGGACGTGGCAGTGCTGGTGCTCCTATTGGCAGTCATCTCCAACACCAGCAAGGCACACAGCAGAATGTAGGAAATCTGGGGTCTAACTTTAACTTTCCTGCTCTGGAAAATCCTAATAGTCAGCCATCTGTGGGCGGTCCATTATCTCAACCTGGATTTGTCAATAATATGCCAGTTCAAGGACCAAGCCAAACATTTCGTGATGGATTTTCCATGGCGGGCATGTCTCAGGAGTTCTTGGGTGATGACTTTAAAAGTCAGGGATCTCATGTTCCTTATAACGTCGCTGACTTTTCCACTCAGGCCTCTCAAAGTGGATATGCTGTTGATTATGTTACACAAGGAGCGCAAGGAGGATTTCCAAGCAATTTTATAAACCAGAATTCTCAAGCAGGGTACTCTCGATTTGGTACAGGGAATGACTTCATGTCTCAGGACTACATGGCTCATGGTACACAAGGTCTATTTACCCAGGTGGGCTACAATGACCCATCACAGGATGATGGATCGCAGAACCACTATGGAGTAGGGAATGCTAATGCGCTTCAGTCTCAGGGATATATGAATTCTCTGTACTCCCAACCTTTTACCCACTACAACACACAGCCAATGAACTTGCAGGCCCCACAACAGCAGCCGCCTCAGCAGGGTCAGAGCTCCCAAAATCAGAAGATTCACTACAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

1276

Amino Acids

139.52

Weight (kDa)

6.35

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF1_Zn_bind PF09416 139 - 292 8.9e-73 RNA helicase (UPF2 interacting domain)
UPF1_1B_dom PF18141 344 - 433 1.8e-26 RNA helicase UPF1, 1B domain
AAA_11 PF13086 489 - 609 2.1e-23 AAA domain
AAA_30 PF13604 489 - 690 5.7e-14 AAA domain
ResIII PF04851 489 - 559 4.9e-06 Type III restriction enzyme, res subunit
AAA_19 PF13245 496 - 690 3.5e-11 AAA domain
AAA_11 PF13086 591 - 692 1e-28 AAA domain
AAA_12 PF13087 701 - 897 1.2e-61 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2517, 3420, 3515
Acc36I ACCTGC 2 cut(s) 2020, 2149
Acc65I GGTACC 1 cut(s) 2966
AccB1I GGYRCC 2 cut(s) 2966, 3013
AccB7I CCANNNNNTGG 5 cut(s) 934, 1056, 2963, 3032, 3071
AccI GTMKAC 1 cut(s) 1638
AccII CGCG 3 cut(s) 120, 186, 192
AclWI GGATC 3 cut(s) 1922, 3412, 3655
AcoI YGGCCR 3 cut(s) 51, 936, 2279
AcsI RAATTY 5 cut(s) 77, 1942, 2192, 3523, 3706
AcuI CTGAAG 6 cut(s) 1267, 1490, 1694, 1877, 2388, 3671
AcvI CACGTG 1 cut(s) 2657
AcyI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
AfeI AGCGCT 1 cut(s) 1854
AflII CTTAAG 2 cut(s) 700, 2404
AflIII ACRYGT 4 cut(s) 510, 1198, 1910, 2654
AhdI GACNNNNNGTC 3 cut(s) 34, 226, 3568
AjiI CACGTC 2 cut(s) 513, 3146
AloI GAACNNNNNNTCC 2 cut(s) 3396, 3428
Alw21I GWGCWC 7 cut(s) 838, 1453, 1470, 2500, 2662, 3163, 3800
Alw26I GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
Alw44I GTGCAC 1 cut(s) 1449
AlwI GGATC 3 cut(s) 1922, 3412, 3655
AlwNI CAGNNNCTG 1 cut(s) 1994
Ama87I CYCGRG 4 cut(s) 256, 276, 289, 3009
Aor51HI AGCGCT 1 cut(s) 1854
AoxI GGCC 9 cut(s) 51, 141, 146, 279, 936, 1396, 2279, 3444, 3762
ApaLI GTGCAC 1 cut(s) 1449
ApoI RAATTY 5 cut(s) 77, 1942, 2192, 3523, 3706
Asp700I GAANNNNTTC 1 cut(s) 3563
Asp718I GGTACC 1 cut(s) 2966
AspLEI GCGC 6 cut(s) 266, 1234, 1619, 1855, 3490, 3685
AsuC2I CCSGG 6 cut(s) 110, 137, 257, 258, 446, 1455
AsuHPI GGTGA 7 cut(s) 527, 1039, 1148, 2057, 2230, 2449, 3395
AsuNHI GCTAGC 1 cut(s) 2339
AvaI CYCGRG 4 cut(s) 256, 276, 289, 3009
BaeGI GKGCMC 3 cut(s) 823, 1453, 3016
BalI TGGCCA 1 cut(s) 2281
BanI GGYRCC 2 cut(s) 2966, 3013
BanII GRGCYC 3 cut(s) 1470, 2939, 3800
BarI GAAGNNNNNNTAC 1 cut(s) 3803
BauI CACGAG 2 cut(s) 2091, 3069
BbrPI CACGTG 1 cut(s) 2657
BbsI GAAGAC 1 cut(s) 560
Bbv12I GWGCWC 7 cut(s) 838, 1453, 1470, 2500, 2662, 3163, 3800
BbvCI CCTCAGC 2 cut(s) 815, 3783
BccI CCATC 9 cut(s) 1392, 1411, 2249, 2311, 2902, 3274, 3338, 3638, 3640
BceAI ACGGC 6 cut(s) 48, 66, 139, 457, 923, 3062
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BclI TGATCA 2 cut(s) 1203, 2047
BcnI CCSGG 6 cut(s) 110, 137, 257, 258, 446, 1455
BcoDI GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
BfaI CTAG 7 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424
BfmI CTRYAG 3 cut(s) 685, 1563, 1635
BfoI RGCGCY 2 cut(s) 267, 1856
BfrI CTTAAG 2 cut(s) 700, 2404
BfuAI ACCTGC 2 cut(s) 2020, 2149
BglI GCCNNNNNGGC 1 cut(s) 272
BglII AGATCT 2 cut(s) 895, 2817
BmeRI GACNNNNNGTC 3 cut(s) 34, 226, 3568
BmeT110I CYCGRG 4 cut(s) 256, 276, 289, 3009
BmgBI CACGTC 2 cut(s) 513, 3146
BmrI ACTGGG 4 cut(s) 378, 739, 1677, 2101
BmsI GCATC 8 cut(s) 223, 611, 871, 943, 1156, 1484, 2185, 2611
BmtI GCTAGC 1 cut(s) 2343
BmuI ACTGGG 4 cut(s) 378, 739, 1677, 2101
BpiI GAAGAC 1 cut(s) 560
BpmI CTGGAG 2 cut(s) 1944, 2877
Bpu10I CCTNAGC 3 cut(s) 737, 815, 3783
BpuEI CTTGAG 3 cut(s) 623, 920, 3513
BpuMI CCSGG 6 cut(s) 110, 137, 257, 258, 446, 1455
BsaAI YACGTR 1 cut(s) 2657
BsaHI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
BsaWI WCCGGW 1 cut(s) 2897
Bse118I RCCGGY 2 cut(s) 281, 423
Bse3DI GCAATG 1 cut(s) 634
BseMI GCAATG 1 cut(s) 634
BseRI GAGGAG 5 cut(s) 213, 216, 1238, 2342, 2605
BseSI GKGCMC 3 cut(s) 823, 1453, 3016
BseYI CCCAGC 1 cut(s) 2096
BsgI GTGCAG 1 cut(s) 1200
Bsh1236I CGCG 3 cut(s) 120, 186, 192
Bsh1285I CGRYCG 2 cut(s) 184, 190
BshFI GGCC 9 cut(s) 53, 143, 148, 281, 938, 1398, 2281, 3446, 3764
BshNI GGYRCC 2 cut(s) 2966, 3013
BsiEI CGRYCG 2 cut(s) 184, 190
BsiHKAI GWGCWC 7 cut(s) 838, 1453, 1470, 2500, 2662, 3163, 3800
BsiHKCI CYCGRG 4 cut(s) 256, 276, 289, 3009
BsmAI GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
BsmI GAATGC 3 cut(s) 2003, 2178, 3679
BsnI GGCC 9 cut(s) 53, 143, 148, 281, 938, 1398, 2281, 3446, 3764
BsoBI CYCGRG 4 cut(s) 256, 276, 289, 3009
Bsp19I CCATGG 2 cut(s) 1276, 3354
BspANI GGCC 9 cut(s) 53, 143, 148, 281, 938, 1398, 2281, 3446, 3764
BspFNI CGCG 3 cut(s) 120, 186, 192
BspMAI CTGCAG 1 cut(s) 1567
BspMI ACCTGC 2 cut(s) 2020, 2149
BspOI GCTAGC 1 cut(s) 2343
BspPI GGATC 3 cut(s) 1922, 3412, 3655
BspT107I GGYRCC 2 cut(s) 2966, 3013
BspTI CTTAAG 2 cut(s) 700, 2404
BsrDI GCAATG 1 cut(s) 634
BsrFI RCCGGY 2 cut(s) 281, 423
BssAI RCCGGY 2 cut(s) 281, 423
BssNI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
BssSI CACGAG 2 cut(s) 2091, 3069
BssT1I CCWWGG 2 cut(s) 1276, 3354
Bst2BI CACGAG 2 cut(s) 2091, 3069
Bst4CI ACNGT 2 cut(s) 617, 1555
Bst6I CTCTTC 2 cut(s) 29, 1298
BstACI GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
BstAFI CTTAAG 2 cut(s) 700, 2404
BstAPI GCANNNNNTGC 2 cut(s) 2093, 3020
BstBAI YACGTR 1 cut(s) 2657
BstDSI CCRYGG 4 cut(s) 190, 1276, 2276, 3354
BstENI CCTNNNNNAGG 1 cut(s) 1542
BstFNI CGCG 3 cut(s) 120, 186, 192
BstH2I RGCGCY 2 cut(s) 267, 1856
BstHHI GCGC 6 cut(s) 266, 1234, 1619, 1855, 3490, 3685
BstMAI GTCTC 5 cut(s) 1352, 2115, 3372, 3576, 3696
BstMCI CGRYCG 2 cut(s) 184, 190
BstNSI RCATGY 4 cut(s) 419, 1202, 1914, 3367
BstSFI CTRYAG 3 cut(s) 685, 1563, 1635
BstSLI GKGCMC 3 cut(s) 823, 1453, 3016
BstUI CGCG 3 cut(s) 120, 186, 192
BstV2I GAAGAC 1 cut(s) 560
BstX2I RGATCY 4 cut(s) 895, 1927, 2817, 3404
BstXI CCANNNNNNTGG 1 cut(s) 2978
BstYI RGATCY 4 cut(s) 895, 1927, 2817, 3404
BsuRI GGCC 9 cut(s) 53, 143, 148, 281, 938, 1398, 2281, 3446, 3764
BtgI CCRYGG 4 cut(s) 190, 1276, 2276, 3354
BtrI CACGTC 2 cut(s) 513, 3146
BtsI GCAGTG 5 cut(s) 785, 1356, 1430, 2050, 3157
BtsIMutI CAGTG 8 cut(s) 366, 408, 749, 785, 1356, 1430, 2050, 3157
BveI ACCTGC 2 cut(s) 2020, 2149
CaiI CAGNNNCTG 1 cut(s) 1994
CfoI GCGC 6 cut(s) 266, 1234, 1619, 1855, 3490, 3685
Cfr10I RCCGGY 2 cut(s) 281, 423
Cfr42I CCGCGG 1 cut(s) 193
Cfr9I CCCGGG 1 cut(s) 256
CseI GACGC 8 cut(s) 24, 68, 225, 324, 335, 2224, 2740, 2822
CsiI ACCWGGT 2 cut(s) 537, 1132
DraI TTTAAA 1 cut(s) 3394
DriI GACNNNNNGTC 3 cut(s) 34, 226, 3568
EaeI YGGCCR 3 cut(s) 51, 936, 2279
Eam1104I CTCTTC 2 cut(s) 29, 1298
Eam1105I GACNNNNNGTC 3 cut(s) 34, 226, 3568
EarI CTCTTC 2 cut(s) 29, 1298
EciI GGCGGA 1 cut(s) 210
Ecl136II GAGCTC 2 cut(s) 1468, 3798
Eco130I CCWWGG 2 cut(s) 1276, 3354
Eco147I AGGCCT 1 cut(s) 3446
Eco24I GRGCYC 3 cut(s) 1470, 2939, 3800
Eco32I GATATC 1 cut(s) 2999
Eco47III AGCGCT 1 cut(s) 1854
Eco53kI GAGCTC 2 cut(s) 1468, 3798
Eco57I CTGAAG 6 cut(s) 1267, 1490, 1694, 1877, 2388, 3671
Eco72I CACGTG 1 cut(s) 2657
Eco88I CYCGRG 4 cut(s) 256, 276, 289, 3009
EcoICRI GAGCTC 2 cut(s) 1468, 3798
EcoNI CCTNNNNNAGG 1 cut(s) 1542
EcoO109I RGGNCCY 4 cut(s) 733, 793, 2765, 3763
EcoRI GAATTC 3 cut(s) 77, 3523, 3706
EcoRV GATATC 1 cut(s) 2999
EcoT14I CCWWGG 2 cut(s) 1276, 3354
EcoT22I ATGCAT 4 cut(s) 715, 958, 1477, 2178
EcoT38I GRGCYC 3 cut(s) 1470, 2939, 3800
ErhI CCWWGG 2 cut(s) 1276, 3354
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FauI CCCGC 3 cut(s) 296, 1854, 3352
FauNDI CATATG 1 cut(s) 2449
FbaI TGATCA 2 cut(s) 1203, 2047
FblI GTMKAC 1 cut(s) 1638
FriOI GRGCYC 3 cut(s) 1470, 2939, 3800
FspBI CTAG 7 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424
GlaI GCGC 6 cut(s) 265, 1233, 1618, 1854, 3489, 3684
GsaI CCCAGC 1 cut(s) 2100
GsuI CTGGAG 2 cut(s) 1944, 2877
HaeII RGCGCY 2 cut(s) 267, 1856
HaeIII GGCC 9 cut(s) 53, 143, 148, 281, 938, 1398, 2281, 3446, 3764
HgaI GACGC 8 cut(s) 24, 68, 225, 324, 335, 2224, 2740, 2822
HhaI GCGC 6 cut(s) 266, 1234, 1619, 1855, 3490, 3685
Hin1I GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
Hin6I GCGC 6 cut(s) 264, 1232, 1617, 1853, 3488, 3683
HinP1I GCGC 6 cut(s) 264, 1232, 1617, 1853, 3488, 3683
HincII GTYRAC 3 cut(s) 325, 1212, 2730
HindII GTYRAC 3 cut(s) 325, 1212, 2730
HindIII AAGCTT 2 cut(s) 992, 1341
HpaI GTTAAC 1 cut(s) 1212
HphI GGTGA 7 cut(s) 527, 1039, 1148, 2057, 2230, 2449, 3395
Hpy99I CGWCG 6 cut(s) 40, 62, 241, 478, 1443, 3428
HpyAV CCTTC 9 cut(s) 265, 647, 712, 1669, 1748, 1798, 2208, 2550, 2756
HpyCH4III ACNGT 2 cut(s) 617, 1555
HpyCH4IV ACGT 5 cut(s) 468, 512, 2656, 3145, 3423
HpySE526I ACGT 5 cut(s) 468, 512, 2656, 3145, 3423
Hsp92I GRCGYC 5 cut(s) 35, 60, 327, 2235, 2833
HspAI GCGC 6 cut(s) 264, 1232, 1617, 1853, 3488, 3683
KpnI GGTACC 1 cut(s) 2970
Ksp22I TGATCA 2 cut(s) 1203, 2047
KspAI GTTAAC 1 cut(s) 1212
KspI CCGCGG 1 cut(s) 193
LweI GCATC 8 cut(s) 223, 611, 871, 943, 1156, 1484, 2185, 2611
MabI ACCWGGT 2 cut(s) 537, 1132
MaeI CTAG 7 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424
MaeII ACGT 5 cut(s) 468, 512, 2656, 3145, 3423
MaeIII GTNAC 6 cut(s) 464, 1555, 1791, 2236, 2392, 3475
MboII GAAGA 9 cut(s) 16, 560, 671, 869, 905, 1315, 1496, 2928, 3823
MfeI CAATTG 2 cut(s) 1569, 1799
MflI RGATCY 4 cut(s) 895, 1927, 2817, 3404
MlsI TGGCCA 1 cut(s) 2281
MluNI TGGCCA 1 cut(s) 2281
MlyI GAGTC 1 cut(s) 1982
MmeI TCCRAC 4 cut(s) 243, 2226, 3089, 3205
Mox20I TGGCCA 1 cut(s) 2281
Mph1103I ATGCAT 4 cut(s) 715, 958, 1477, 2178
MroXI GAANNNNTTC 1 cut(s) 3563
MscI TGGCCA 1 cut(s) 2281
MslI CAYNNNNRTG 1 cut(s) 3822
Msp20I TGGCCA 1 cut(s) 2281
MspA1I CMGCKG 3 cut(s) 192, 814, 1265
MspCI CTTAAG 2 cut(s) 700, 2404
MunI CAATTG 2 cut(s) 1569, 1799
Mva1269I GAATGC 3 cut(s) 2003, 2178, 3679
MvnI CGCG 3 cut(s) 120, 186, 192
NciI CCSGG 6 cut(s) 110, 137, 257, 258, 446, 1455
NcoI CCATGG 2 cut(s) 1276, 3354
NdeI CATATG 1 cut(s) 2449
NheI GCTAGC 1 cut(s) 2339
NmuCI GTSAC 3 cut(s) 1555, 2236, 2392
NsiI ATGCAT 4 cut(s) 715, 958, 1477, 2178
NspI RCATGY 4 cut(s) 419, 1202, 1914, 3367
PaeI GCATGC 1 cut(s) 419
PasI CCCWGGG 1 cut(s) 88
PceI AGGCCT 1 cut(s) 3446
PciI ACATGT 2 cut(s) 1198, 1910
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PctI GAATGC 3 cut(s) 2003, 2178, 3679
PdmI GAANNNNTTC 1 cut(s) 3563
PflMI CCANNNNNTGG 5 cut(s) 934, 1056, 2963, 3032, 3071
PfoI TCCNGGA 1 cut(s) 2854
PleI GAGTC 1 cut(s) 1981
PmaCI CACGTG 1 cut(s) 2657
PmlI CACGTG 1 cut(s) 2657
PpsI GAGTC 1 cut(s) 1981
Ppu21I YACGTR 1 cut(s) 2657
PpuMI RGGWCCY 3 cut(s) 733, 793, 2765
PscI ACATGT 2 cut(s) 1198, 1910
PsiI TTATAA 3 cut(s) 2517, 3420, 3515
Psp124BI GAGCTC 2 cut(s) 1470, 3800
Psp5II RGGWCCY 3 cut(s) 733, 793, 2765
PspCI CACGTG 1 cut(s) 2657
PspFI CCCAGC 1 cut(s) 2096
PspPPI RGGWCCY 3 cut(s) 733, 793, 2765
PsrI GAACNNNNNNTAC 2 cut(s) 3084, 3116
PstI CTGCAG 1 cut(s) 1567
PstNI CAGNNNCTG 1 cut(s) 1994
PsuI RGATCY 4 cut(s) 895, 1927, 2817, 3404
PvuII CAGCTG 2 cut(s) 814, 1265
RseI CAYNNNNRTG 1 cut(s) 3822
SacI GAGCTC 2 cut(s) 1470, 3800
SacII CCGCGG 1 cut(s) 193
SchI GAGTC 1 cut(s) 1982
SexAI ACCWGGT 2 cut(s) 537, 1132
SfaNI GCATC 8 cut(s) 223, 611, 871, 943, 1156, 1484, 2185, 2611
SfcI CTRYAG 3 cut(s) 685, 1563, 1635
Sfr303I CCGCGG 1 cut(s) 193
SgrBI CCGCGG 1 cut(s) 193
SmaI CCCGGG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 3822
SmlI CTYRAG 5 cut(s) 602, 700, 899, 2404, 3528
SmoI CTYRAG 5 cut(s) 602, 700, 899, 2404, 3528
SphI GCATGC 1 cut(s) 419
SseBI AGGCCT 1 cut(s) 3446
SspMI CTAG 7 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424
SstI GAGCTC 2 cut(s) 1470, 3800
StuI AGGCCT 1 cut(s) 3446
StyI CCWWGG 2 cut(s) 1276, 3354
TaaI ACNGT 2 cut(s) 617, 1555
TaiI ACGT 5 cut(s) 471, 515, 2659, 3148, 3426
TaqI TCGA 9 cut(s) 27, 75, 102, 440, 1236, 1933, 2760, 3127, 3544
TaqII GACCGA 1 cut(s) 120
TatI WGTACW 3 cut(s) 1307, 1845, 3714
TauI GCSGC 6 cut(s) 195, 303, 435, 627, 1150, 3781
TscAI CASTG 8 cut(s) 373, 415, 749, 785, 1356, 1437, 2057, 3157
TseFI GTSAC 3 cut(s) 1555, 2236, 2392
Tsp45I GTSAC 3 cut(s) 1555, 2236, 2392
TspGWI ACGGA 3 cut(s) 1412, 1943, 2265
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 8 cut(s) 373, 415, 749, 785, 1356, 1437, 2057, 3157
Van91I CCANNNNNTGG 5 cut(s) 934, 1056, 2963, 3032, 3071
Vha464I CTTAAG 2 cut(s) 700, 2404
VneI GTGCAC 1 cut(s) 1449
XagI CCTNNNNNAGG 1 cut(s) 1542
XapI RAATTY 5 cut(s) 77, 1942, 2192, 3523, 3706
XceI RCATGY 4 cut(s) 419, 1202, 1914, 3367
XmaI CCCGGG 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 1638
XmnI GAANNNNTTC 1 cut(s) 3563
XspI CTAG 7 cut(s) 332, 1248, 1623, 1646, 2018, 2340, 2424
Zsp2I ATGCAT 4 cut(s) 715, 958, 1477, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.