WAT1-related protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 601 |
| AciI | CCGC | 6 cut(s) | 114, 171, 566, 875, 1076, 1259 |
| AclWI | GGATC | 1 cut(s) | 280 |
| AcoI | YGGCCR | 3 cut(s) | 111, 318, 372 |
| AcsI | RAATTY | 2 cut(s) | 286, 400 |
| AcuI | CTGAAG | 1 cut(s) | 367 |
| AfaI | GTAC | 4 cut(s) | 13, 237, 293, 1125 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 820, 999 |
| AflII | CTTAAG | 1 cut(s) | 133 |
| AgsI | TTSAA | 7 cut(s) | 46, 203, 299, 400, 762, 967, 1242 |
| AhlI | ACTAGT | 1 cut(s) | 1250 |
| AjnI | CCWGG | 2 cut(s) | 648, 795 |
| AluBI | AGCT | 4 cut(s) | 303, 356, 618, 1137 |
| AluI | AGCT | 4 cut(s) | 303, 356, 618, 1137 |
| Alw21I | GWGCWC | 1 cut(s) | 987 |
| Alw26I | GTCTC | 1 cut(s) | 678 |
| AlwI | GGATC | 1 cut(s) | 280 |
| Ama87I | CYCGRG | 1 cut(s) | 883 |
| AoxI | GGCC | 8 cut(s) | 111, 215, 318, 372, 471, 606, 798, 943 |
| ApaI | GGGCCC | 1 cut(s) | 947 |
| ApoI | RAATTY | 2 cut(s) | 286, 400 |
| AseI | ATTAAT | 1 cut(s) | 443 |
| AspS9I | GGNCC | 3 cut(s) | 504, 943, 944 |
| AsuHPI | GGTGA | 2 cut(s) | 370, 593 |
| AvaI | CYCGRG | 1 cut(s) | 883 |
| AvaII | GGWCC | 1 cut(s) | 504 |
| BaeGI | GKGCMC | 1 cut(s) | 947 |
| BalI | TGGCCA | 1 cut(s) | 374 |
| BanII | GRGCYC | 2 cut(s) | 947, 1223 |
| Bbv12I | GWGCWC | 1 cut(s) | 987 |
| BccI | CCATC | 5 cut(s) | 116, 226, 383, 672, 1001 |
| BceAI | ACGGC | 1 cut(s) | 785 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1195, 1229 |
| BciT130I | CCWGG | 2 cut(s) | 650, 797 |
| BcoDI | GTCTC | 1 cut(s) | 678 |
| BcuI | ACTAGT | 1 cut(s) | 1250 |
| BfaI | CTAG | 2 cut(s) | 848, 1251 |
| BfmI | CTRYAG | 1 cut(s) | 665 |
| BfrI | CTTAAG | 1 cut(s) | 133 |
| BisI | GCNGC | 2 cut(s) | 114, 876 |
| BlpI | GCTNAGC | 1 cut(s) | 304 |
| BlsI | GCNGC | 2 cut(s) | 115, 877 |
| Bme1390I | CCNGG | 2 cut(s) | 650, 797 |
| Bme18I | GGWCC | 1 cut(s) | 504 |
| BmeT110I | CYCGRG | 1 cut(s) | 883 |
| BmgT120I | GGNCC | 3 cut(s) | 504, 943, 944 |
| BmiI | GGNNCC | 4 cut(s) | 169, 836, 944, 945 |
| BmrFI | CCNGG | 2 cut(s) | 650, 797 |
| BmrI | ACTGGG | 1 cut(s) | 909 |
| BmsI | GCATC | 3 cut(s) | 566, 834, 1036 |
| BmuI | ACTGGG | 1 cut(s) | 909 |
| BpmI | CTGGAG | 2 cut(s) | 410, 1167 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 304 |
| BsaBI | GATNNNNATC | 1 cut(s) | 277 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 815, 845 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 820, 999 |
| Bse1I | ACTGG | 5 cut(s) | 19, 50, 114, 609, 904 |
| Bse3DI | GCAATG | 3 cut(s) | 468, 789, 987 |
| Bse8I | GATNNNNATC | 1 cut(s) | 277 |
| BseBI | CCWGG | 2 cut(s) | 650, 797 |
| BseGI | GGATG | 3 cut(s) | 237, 348, 849 |
| BseJI | GATNNNNATC | 1 cut(s) | 277 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 820, 999 |
| BseMI | GCAATG | 3 cut(s) | 468, 789, 987 |
| BseNI | ACTGG | 5 cut(s) | 19, 50, 114, 609, 904 |
| BseSI | GKGCMC | 1 cut(s) | 947 |
| BseYI | CCCAGC | 1 cut(s) | 352 |
| BshFI | GGCC | 8 cut(s) | 113, 217, 320, 374, 473, 608, 800, 945 |
| BsiHKAI | GWGCWC | 1 cut(s) | 987 |
| BsiHKCI | CYCGRG | 1 cut(s) | 883 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 820, 999 |
| BsmAI | GTCTC | 1 cut(s) | 678 |
| BsmBI | CGTCTC | 1 cut(s) | 678 |
| BsnI | GGCC | 8 cut(s) | 113, 217, 320, 374, 473, 608, 800, 945 |
| BsoBI | CYCGRG | 1 cut(s) | 883 |
| Bsp120I | GGGCCC | 1 cut(s) | 943 |
| Bsp1286I | GDGCHC | 3 cut(s) | 947, 987, 1223 |
| Bsp1407I | TGTACA | 1 cut(s) | 291 |
| Bsp143I | GATC | 2 cut(s) | 272, 583 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 304 |
| BspACI | CCGC | 6 cut(s) | 114, 171, 566, 875, 1076, 1259 |
| BspANI | GGCC | 8 cut(s) | 113, 217, 320, 374, 473, 608, 800, 945 |
| BspLI | GGNNCC | 4 cut(s) | 169, 836, 944, 945 |
| BspPI | GGATC | 1 cut(s) | 280 |
| BspTI | CTTAAG | 1 cut(s) | 133 |
| BsrDI | GCAATG | 3 cut(s) | 468, 789, 987 |
| BsrGI | TGTACA | 1 cut(s) | 291 |
| BsrI | ACTGG | 5 cut(s) | 19, 50, 114, 609, 904 |
| BssMI | GATC | 2 cut(s) | 272, 583 |
| Bst2UI | CCWGG | 2 cut(s) | 650, 797 |
| Bst4CI | ACNGT | 1 cut(s) | 1128 |
| BstAFI | CTTAAG | 1 cut(s) | 133 |
| BstAUI | TGTACA | 1 cut(s) | 291 |
| BstDEI | CTNAG | 1 cut(s) | 304 |
| BstF5I | GGATG | 3 cut(s) | 237, 348, 849 |
| BstKTI | GATC | 2 cut(s) | 275, 586 |
| BstMAI | GTCTC | 1 cut(s) | 678 |
| BstMBI | GATC | 2 cut(s) | 272, 583 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 326, 942, 1244 |
| BstNI | CCWGG | 2 cut(s) | 650, 797 |
| BstNSI | RCATGY | 1 cut(s) | 515 |
| BstSCI | CCNGG | 2 cut(s) | 648, 795 |
| BstSFI | CTRYAG | 1 cut(s) | 665 |
| BstSLI | GKGCMC | 1 cut(s) | 947 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 803 |
| BsuRI | GGCC | 8 cut(s) | 113, 217, 320, 374, 473, 608, 800, 945 |
| BtgZI | GCGATG | 1 cut(s) | 20 |
| BtsCI | GGATG | 3 cut(s) | 237, 348, 849 |
| BtsI | GCAGTG | 2 cut(s) | 765, 1056 |
| BtsIMutI | CAGTG | 4 cut(s) | 57, 765, 897, 1056 |
| Cfr13I | GGNCC | 3 cut(s) | 504, 943, 944 |
| Csp6I | GTAC | 4 cut(s) | 12, 236, 292, 1124 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 496, 531 |
| CviAII | CATG | 6 cut(s) | 38, 228, 512, 820, 937, 1267 |
| CviQI | GTAC | 4 cut(s) | 12, 236, 292, 1124 |
| DdeI | CTNAG | 1 cut(s) | 304 |
| DpnI | GATC | 2 cut(s) | 274, 585 |
| DpnII | GATC | 2 cut(s) | 272, 583 |
| EaeI | YGGCCR | 3 cut(s) | 111, 318, 372 |
| Eco24I | GRGCYC | 2 cut(s) | 947, 1223 |
| Eco47I | GGWCC | 1 cut(s) | 504 |
| Eco57I | CTGAAG | 1 cut(s) | 367 |
| Eco88I | CYCGRG | 1 cut(s) | 883 |
| EcoO109I | RGGNCCY | 2 cut(s) | 943, 944 |
| EcoRII | CCWGG | 2 cut(s) | 648, 795 |
| EcoT38I | GRGCYC | 2 cut(s) | 947, 1223 |
| Esp3I | CGTCTC | 1 cut(s) | 678 |
| FaeI | CATG | 6 cut(s) | 41, 231, 515, 823, 940, 1270 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 754, 786 |
| FatI | CATG | 6 cut(s) | 37, 227, 511, 819, 936, 1266 |
| FblI | GTMKAC | 1 cut(s) | 601 |
| Fnu4HI | GCNGC | 2 cut(s) | 114, 876 |
| FokI | GGATG | 3 cut(s) | 244, 335, 856 |
| FriOI | GRGCYC | 2 cut(s) | 947, 1223 |
| Fsp4HI | GCNGC | 2 cut(s) | 114, 876 |
| FspBI | CTAG | 2 cut(s) | 848, 1251 |
| GluI | GCNGC | 2 cut(s) | 114, 876 |
| GsaI | CCCAGC | 1 cut(s) | 356 |
| GsuI | CTGGAG | 2 cut(s) | 410, 1167 |
| HaeIII | GGCC | 8 cut(s) | 113, 217, 320, 374, 473, 608, 800, 945 |
| Hin1II | CATG | 6 cut(s) | 41, 231, 515, 823, 940, 1270 |
| HincII | GTYRAC | 3 cut(s) | 517, 1131, 1201 |
| HindII | GTYRAC | 3 cut(s) | 517, 1131, 1201 |
| HindIII | AAGCTT | 2 cut(s) | 301, 1135 |
| HinfI | GANTC | 5 cut(s) | 139, 575, 1114, 1151, 1270 |
| HphI | GGTGA | 2 cut(s) | 370, 593 |
| Hpy166II | GTNNAC | 5 cut(s) | 507, 517, 602, 1131, 1201 |
| Hpy188I | TCNGA | 1 cut(s) | 614 |
| Hpy188III | TCNNGA | 4 cut(s) | 885, 1148, 1184, 1216 |
| Hpy8I | GTNNAC | 5 cut(s) | 507, 517, 602, 1131, 1201 |
| HpyAV | CCTTC | 7 cut(s) | 184, 188, 373, 391, 415, 421, 619 |
| HpyCH4III | ACNGT | 1 cut(s) | 1128 |
| HpyCH4V | TGCA | 8 cut(s) | 37, 80, 86, 149, 244, 819, 930, 1027 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 326, 942, 1244 |
| HpyF3I | CTNAG | 1 cut(s) | 304 |
| Hsp92II | CATG | 6 cut(s) | 41, 231, 515, 823, 940, 1270 |
| Kzo9I | GATC | 2 cut(s) | 272, 583 |
| LmnI | GCTCC | 3 cut(s) | 458, 834, 1046 |
| LweI | GCATC | 3 cut(s) | 566, 834, 1036 |
| MaeI | CTAG | 2 cut(s) | 848, 1251 |
| MaeIII | GTNAC | 3 cut(s) | 626, 739, 895 |
| MalI | GATC | 2 cut(s) | 274, 585 |
| MboI | GATC | 2 cut(s) | 272, 583 |
| MboII | GAAGA | 3 cut(s) | 173, 220, 1120 |
| MfeI | CAATTG | 1 cut(s) | 1161 |
| MhlI | GDGCHC | 3 cut(s) | 947, 987, 1223 |
| MlsI | TGGCCA | 1 cut(s) | 374 |
| MluCI | AATT | 6 cut(s) | 286, 395, 400, 659, 888, 1161 |
| MluNI | TGGCCA | 1 cut(s) | 374 |
| MlyI | GAGTC | 1 cut(s) | 1123 |
| MnlI | CCTC | 6 cut(s) | 475, 819, 824, 933, 981, 1191 |
| Mox20I | TGGCCA | 1 cut(s) | 374 |
| MscI | TGGCCA | 1 cut(s) | 374 |
| MseI | TTAA | 6 cut(s) | 59, 93, 134, 311, 443, 773 |
| MslI | CAYNNNNRTG | 2 cut(s) | 230, 935 |
| Msp20I | TGGCCA | 1 cut(s) | 374 |
| MspA1I | CMGCKG | 1 cut(s) | 875 |
| MspCI | CTTAAG | 1 cut(s) | 133 |
| MspR9I | CCNGG | 2 cut(s) | 650, 797 |
| MunI | CAATTG | 1 cut(s) | 1161 |
| MvaI | CCWGG | 2 cut(s) | 650, 797 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 326, 942, 1244 |
| NdeII | GATC | 2 cut(s) | 272, 583 |
| NlaIII | CATG | 6 cut(s) | 41, 231, 515, 823, 940, 1270 |
| NlaIV | GGNNCC | 4 cut(s) | 169, 836, 944, 945 |
| NmuCI | GTSAC | 2 cut(s) | 739, 895 |
| NspI | RCATGY | 1 cut(s) | 515 |
| PfeI | GAWTC | 4 cut(s) | 139, 575, 1151, 1270 |
| PkrI | GCNGC | 2 cut(s) | 115, 877 |
| PleI | GAGTC | 1 cut(s) | 1122 |
| PpsI | GAGTC | 1 cut(s) | 1122 |
| PshBI | ATTAAT | 1 cut(s) | 443 |
| Psp6I | CCWGG | 2 cut(s) | 648, 795 |
| PspFI | CCCAGC | 1 cut(s) | 352 |
| PspGI | CCWGG | 2 cut(s) | 648, 795 |
| PspN4I | GGNNCC | 4 cut(s) | 169, 836, 944, 945 |
| PspOMI | GGGCCC | 1 cut(s) | 943 |
| PspPI | GGNCC | 3 cut(s) | 504, 943, 944 |
| RsaI | GTAC | 4 cut(s) | 13, 237, 293, 1125 |
| RsaNI | GTAC | 4 cut(s) | 12, 236, 292, 1124 |
| RseI | CAYNNNNRTG | 2 cut(s) | 230, 935 |
| SaqAI | TTAA | 6 cut(s) | 59, 93, 134, 311, 443, 773 |
| SatI | GCNGC | 2 cut(s) | 114, 876 |
| Sau3AI | GATC | 2 cut(s) | 272, 583 |
| Sau96I | GGNCC | 3 cut(s) | 504, 943, 944 |
| SchI | GAGTC | 1 cut(s) | 1123 |
| ScrFI | CCNGG | 2 cut(s) | 650, 797 |
| SduI | GDGCHC | 3 cut(s) | 947, 987, 1223 |
| SfaNI | GCATC | 3 cut(s) | 566, 834, 1036 |
| SfcI | CTRYAG | 1 cut(s) | 665 |
| SinI | GGWCC | 1 cut(s) | 504 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 230, 935 |
| SmlI | CTYRAG | 1 cut(s) | 133 |
| SmoI | CTYRAG | 1 cut(s) | 133 |
| SpeI | ACTAGT | 1 cut(s) | 1250 |
| Sse9I | AATT | 6 cut(s) | 286, 395, 400, 659, 888, 1161 |
| SsiI | CCGC | 6 cut(s) | 114, 171, 566, 875, 1076, 1259 |
| SspI | AATATT | 1 cut(s) | 97 |
| SspMI | CTAG | 2 cut(s) | 848, 1251 |
| StyD4I | CCNGG | 2 cut(s) | 648, 795 |
| TaaI | ACNGT | 1 cut(s) | 1128 |
| TaqI | TCGA | 2 cut(s) | 586, 1215 |
| TasI | AATT | 6 cut(s) | 286, 395, 400, 659, 888, 1161 |
| TatI | WGTACW | 1 cut(s) | 291 |
| TauI | GCSGC | 2 cut(s) | 116, 878 |
| TfiI | GAWTC | 4 cut(s) | 139, 575, 1151, 1270 |
| Tru1I | TTAA | 6 cut(s) | 59, 93, 134, 311, 443, 773 |
| Tru9I | TTAA | 6 cut(s) | 59, 93, 134, 311, 443, 773 |
| TscAI | CASTG | 4 cut(s) | 57, 772, 904, 1056 |
| TseFI | GTSAC | 2 cut(s) | 739, 895 |
| Tsp45I | GTSAC | 2 cut(s) | 739, 895 |
| TspDTI | ATGAA | 6 cut(s) | 143, 148, 187, 355, 1130, 1262 |
| TspRI | CASTG | 4 cut(s) | 57, 772, 904, 1056 |
| Vha464I | CTTAAG | 1 cut(s) | 133 |
| VpaK11BI | GGWCC | 1 cut(s) | 504 |
| VspI | ATTAAT | 1 cut(s) | 443 |
| XapI | RAATTY | 2 cut(s) | 286, 400 |
| XceI | RCATGY | 1 cut(s) | 515 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 225 |
| XmiI | GTMKAC | 1 cut(s) | 601 |
| XspI | CTAG | 2 cut(s) | 848, 1251 |