Rmu_sc0008008.1_g000015

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008008.1
OG0000337
Physical Location & Seq
Forward (+)
41993 .. 46103
4111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008008.1_g000015.1.cds

Sequence Viewer

Length: 1854 bp
atggggcagcagggtggtattgagatttctagcggtcctagtagtgtagtagatggcttaatgaagccggtgatatcgatggtgatcgttcaaatttcatatgcagcaatgaatatattttgcaaacttgcagcaaacgccggaatgaatctcaatattctagttgcctaccgtctcatgttttcctctgcctttatcgttcctcttgctcttatactcgaaaggaacagcaggcccaaactcaccctatttgtactctttcaagcttttctctgcgaggcactggtcttgacatcttcgacatttgttgcagccagtgcaaatctgattccggcggtcaccttcatcatggccatctgtttcaggttggagaagttgacactaggaagccatgcagggaaggcaaagcttgtggggacagtgatgggtatcggtggtgctatgatctttaccttcgtcaaaggaccagagttctccatctggtcaactcacattgaccttcttcgaggtcaccaggcggcagcaccatcaccgtcaacgtcctcctcccacagaagcaccggtagttacttgctgggctgcttcttggctctcggatgcaccgtttcctttgcaatgtggctcatacttcagtcggtagtttttgcactctgcgtggagagggattggagccagtggaaattgggttggaacatcaagcttcttgcagctgcatattcgggaattgtgacttcggggatggcggtggttcttgtctcgtggtgtgtacgcaagagaggtccattgttcgtgtcaatatttactccgctctgcttggtgattgtggccattgctagttctttgttgctcaatgaaaagttaagcctaggaagtagtgtagtagatggcttaatgaagccggtgatatcgatggtgatcgttcaaatttcatatgcagcaatgaatatattttgcaaacttgcagcaaacgccggaatgaatctcaatattctagttgcctaccgtctcatgttttcctctgcctttatcgttcctcttgctcttatactcgaaaggaacagcaggcccaaactcaccctatttgtactctttcaagcttttctctgcgaggcactggtcttgacatcttcgacatttgttgcagccagtgcaaatctgattccggcggtcaccttcatcatggccatctgtttcaggttggagaagttgacactaggaagccatgcagggaaggcaaagcttgtggggacagtgatgggtatcggtggtgctatgatctttaccttcgtcaaaggaccagagttctccatctggtcaactcacattgaccttcttcgaggtcaccaggcggcagcaccatcaccgtcaacgtcctcctcccacagaagcaccggtagttacttgctgggctgcttcttggctctcggatgcaccgtttcctttgcaatgtggctcatacttcagtcggtagtttttgcactctgcgtggagagggattggagccagtggaaattgggttggaacatcaagcttcttgcagctgcatattcgggaattgtaacttcggggatggcggtggttcttgtctcgtggtgtgtacgcaagagaggtccattgttcgtgtcaatattcactccgctctgcttggtgattgtggccattgctagttctttgttgctcaatgaaaagttaagcctaggaagtgatcaagaacaaccagccgcagcagcagcactaagatcttcaacgagactccaagattcccaattcattgaaatttttgcaactcaagcaccggacactaataccttcggtgacaataacagcaacacaaatgttgtgtctagtagttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

617

Amino Acids

66.09

Weight (kDa)

9.32

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25270 AT1G68170 AT1G68170
fragaria_vesca FvH4_5g30250 FvH4_5g30250 FvH4_5g30280 FvH4_5g30370 FvH4_5g30371 FvH4_7g27400 FvH4_7g27400 FvH4_7g27430
malus_domestica MD08G1223600.v1.1 MD08G1224100.v1.1 MD08G1224300.v1.1 MD08G1224400.v1.1 MD08G1224500.v1.1 MD11G1289900.v1.1 MD11G1290000.v1.1 MD15G1417300.v1.1 MD15G1417400.v1.1 MD15G1417500.v1.1 MD17G1230500.v1.1
prunus_persica Prupe.1G561200_v2.0.a1 Prupe.1G561300_v2.0.a1 Prupe.1G561400_v2.0.a1 Prupe.1G561500_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G041900_v2.0.a1 Prupe.7G042100_v2.0.a1
pyrus_communis pycom08g19530 pycom15g37030 pycom15g37040 pycom17g23340
rosa_chinensis RchiOBHm_Chr1g0374241 RchiOBHm_Chr7g0224261 RchiOBHm_Chr7g0224561 RchiOBHm_Chr7g0224591 RchiOBHm_Chr7g0224611 RchiOBHm_Chr7g0224641 RchiOBHm_Chr7g0224801 RchiOBHm_Chr7g0224831
rosa_laevigata RLG00000001850 RLG00000001853 RLG00000001869 RLG00000001876 RLG00000001880 RLG00000001884 RLG00000001909 RLG00000026752
rosa_multiflora Rmu_co8280549.1_g000001 Rmu_co8423019.1_g000001 Rmu_co8464247.1_g000001 Rmu_sc0000144.1_g000002 Rmu_sc0000144.1_g000055 Rmu_sc0007613.1_g000002 Rmu_sc0008008.1_g000015 Rmu_sc0010368.1_g000011 Rmu_sc0010368.1_g000019
rosa_roxburghii Rroxscaffold_3G00234350 Rroxscaffold_3G00234370 Rroxscaffold_3G00234560 Rroxscaffold_3G00234570 Rroxscaffold_3G00234590 Rroxscaffold_3G00234630 Rroxscaffold_4G00316910
rosa_rugosa Rorug01G0381800 Rorug07G0221700 Rorug07G0223900 Rorug07G0224000 Rorug07G0224100 Rorug07G0224200 Rorug07G0224500 Rorug07G0225900 Rorug07G0225900 Rorug07G0226100 Rorug07G0226100 Rorug07G0226200 Rorug07G0226300
rosa_samantha Rh1AG392800 Rh1BG356900 Rh1CG369900 Rh1DG387400 Rh7AG366800 Rh7AG377200 Rh7AG377300 Rh7AG378400 Rh7AG378600 Rh7AG378700 Rh7CG385300 Rh7CG395900 Rh7CG396000 Rh7CG397700 Rh7CG398000 Rh7CG398200 Rh7CG398300 Rh7DG360700 Rh7DG369900 Rh7DG370300 Rh7DG370500 Rh7DG370800 Rh7DG371600 Rh7DG371700
rosa_wichuraiana Rw1G034300 Rw7G031030 Rw7G031250 Rw7G031260 Rw7G031270 Rw7G031290 Rw7G031370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 808, 1639
AcoI YGGCCR 4 cut(s) 351, 825, 1182, 1656
AcsI RAATTY 3 cut(s) 93, 924, 1776
AcuI CTGAAG 2 cut(s) 614, 1445
AfaI GTAC 4 cut(s) 255, 768, 1086, 1599
AgeI ACCGGT 2 cut(s) 560, 1391
AgsI TTSAA 6 cut(s) 92, 263, 923, 1094, 1746, 1775
AjnI CCWGG 2 cut(s) 513, 1344
AluBI AGCT 8 cut(s) 266, 409, 700, 710, 1097, 1240, 1531, 1541
AluI AGCT 8 cut(s) 266, 409, 700, 710, 1097, 1240, 1531, 1541
Alw26I GTCTC 5 cut(s) 179, 760, 1010, 1591, 1744
AoxI GGCC 6 cut(s) 233, 351, 825, 1064, 1182, 1656
ApoI RAATTY 3 cut(s) 93, 924, 1776
ArsI GACNNNNNNTTYG 1 cut(s) 1826
AsiGI ACCGGT 2 cut(s) 560, 1391
AspA2I CCTAGG 2 cut(s) 865, 1696
AspS9I GGNCC 7 cut(s) 35, 234, 464, 779, 1065, 1295, 1610
AvaII GGWCC 5 cut(s) 35, 464, 779, 1295, 1610
AvrII CCTAGG 2 cut(s) 865, 1696
BalI TGGCCA 4 cut(s) 353, 827, 1184, 1658
BauI CACGAG 2 cut(s) 757, 1588
BciT130I CCWGG 2 cut(s) 515, 1346
BclI TGATCA 1 cut(s) 1705
BcoDI GTCTC 5 cut(s) 179, 760, 1010, 1591, 1744
BglII AGATCT 1 cut(s) 1739
BlnI CCTAGG 2 cut(s) 865, 1696
Bme1390I CCNGG 2 cut(s) 515, 1346
Bme18I GGWCC 5 cut(s) 35, 464, 779, 1295, 1610
BmgT120I GGNCC 7 cut(s) 35, 234, 464, 779, 1065, 1295, 1610
BmiI GGNNCC 2 cut(s) 671, 1502
BmrFI CCNGG 2 cut(s) 515, 1346
BmsI GCATC 2 cut(s) 587, 1418
BpuEI CTTGAG 1 cut(s) 1773
Bsa29I ATCGAT 2 cut(s) 77, 908
BsaBI GATNNNNATC 4 cut(s) 83, 428, 914, 1259
BsaJI CCNNGG 2 cut(s) 865, 1696
BsaWI WCCGGW 3 cut(s) 560, 1391, 1795
Bse118I RCCGGY 4 cut(s) 67, 560, 898, 1391
Bse1I ACTGG 6 cut(s) 288, 315, 673, 1119, 1146, 1504
Bse3DI GCAATG 6 cut(s) 114, 621, 828, 945, 1452, 1659
Bse8I GATNNNNATC 4 cut(s) 83, 428, 914, 1259
BseBI CCWGG 2 cut(s) 515, 1346
BseCI ATCGAT 2 cut(s) 77, 908
BseDI CCNNGG 2 cut(s) 865, 1696
BseGI GGATG 4 cut(s) 602, 744, 1433, 1575
BseJI GATNNNNATC 4 cut(s) 83, 428, 914, 1259
BseMI GCAATG 6 cut(s) 114, 621, 828, 945, 1452, 1659
BseNI ACTGG 6 cut(s) 288, 315, 673, 1119, 1146, 1504
BseRI GAGGAG 2 cut(s) 535, 1366
BseYI CCCAGC 2 cut(s) 574, 1405
BshFI GGCC 6 cut(s) 235, 353, 827, 1066, 1184, 1658
BshTI ACCGGT 2 cut(s) 560, 1391
BshVI ATCGAT 2 cut(s) 77, 908
BsiSI CCGG 9 cut(s) 68, 141, 332, 561, 899, 972, 1163, 1392, 1796
BslFI GGGAC 2 cut(s) 430, 1261
BsmAI GTCTC 5 cut(s) 179, 760, 1010, 1591, 1744
BsmBI CGTCTC 2 cut(s) 179, 1010
BsmFI GGGAC 2 cut(s) 430, 1261
BsnI GGCC 6 cut(s) 235, 353, 827, 1066, 1184, 1658
Bsp143I GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
BspANI GGCC 6 cut(s) 235, 353, 827, 1066, 1184, 1658
BspDI ATCGAT 2 cut(s) 77, 908
BspLI GGNNCC 2 cut(s) 671, 1502
BsrBI CCGCTC 2 cut(s) 808, 1639
BsrDI GCAATG 6 cut(s) 114, 621, 828, 945, 1452, 1659
BsrFI RCCGGY 4 cut(s) 67, 560, 898, 1391
BsrI ACTGG 6 cut(s) 288, 315, 673, 1119, 1146, 1504
BssAI RCCGGY 4 cut(s) 67, 560, 898, 1391
BssECI CCNNGG 2 cut(s) 865, 1696
BssMI GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
BssSI CACGAG 2 cut(s) 757, 1588
BssT1I CCWWGG 2 cut(s) 865, 1696
Bst2BI CACGAG 2 cut(s) 757, 1588
Bst2UI CCWGG 2 cut(s) 515, 1346
Bst4CI ACNGT 8 cut(s) 173, 421, 534, 604, 1004, 1252, 1365, 1435
BstAPI GCANNNNNTGC 2 cut(s) 317, 1148
BstC8I GCNNGC 2 cut(s) 233, 1064
BstDEI CTNAG 1 cut(s) 1736
BstEII GGTNACC 4 cut(s) 337, 509, 1168, 1340
BstF5I GGATG 4 cut(s) 602, 744, 1433, 1575
BstKTI GATC 6 cut(s) 87, 447, 918, 1278, 1708, 1742
BstMAI GTCTC 5 cut(s) 179, 760, 1010, 1591, 1744
BstMBI GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
BstMWI GCNNNNNNNGC 9 cut(s) 137, 317, 401, 968, 1148, 1232, 1727, 1730, 1790
BstNI CCWGG 2 cut(s) 515, 1346
BstPI GGTNACC 4 cut(s) 337, 509, 1168, 1340
BstSCI CCNGG 2 cut(s) 513, 1344
BstX2I RGATCY 1 cut(s) 1739
BstYI RGATCY 1 cut(s) 1739
Bsu15I ATCGAT 2 cut(s) 77, 908
BsuRI GGCC 6 cut(s) 235, 353, 827, 1066, 1184, 1658
BsuTUI ATCGAT 2 cut(s) 77, 908
BtsCI GGATG 4 cut(s) 602, 744, 1433, 1575
BtsIMutI CAGTG 8 cut(s) 281, 322, 426, 680, 1112, 1153, 1257, 1511
Cac8I GCNNGC 2 cut(s) 233, 1064
Cfr10I RCCGGY 4 cut(s) 67, 560, 898, 1391
Cfr13I GGNCC 7 cut(s) 35, 234, 464, 779, 1065, 1295, 1610
ClaI ATCGAT 2 cut(s) 77, 908
Csp6I GTAC 4 cut(s) 254, 767, 1085, 1598
CspAI ACCGGT 2 cut(s) 560, 1391
CspCI CAANNNNNGTGG 4 cut(s) 393, 428, 1224, 1259
CviAII CATG 6 cut(s) 178, 349, 392, 1009, 1180, 1223
CviQI GTAC 4 cut(s) 254, 767, 1085, 1598
DdeI CTNAG 1 cut(s) 1736
DpnI GATC 6 cut(s) 86, 446, 917, 1277, 1707, 1741
DpnII GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
EaeI YGGCCR 4 cut(s) 351, 825, 1182, 1656
Eco130I CCWWGG 2 cut(s) 865, 1696
Eco32I GATATC 2 cut(s) 75, 906
Eco47I GGWCC 5 cut(s) 35, 464, 779, 1295, 1610
Eco57I CTGAAG 2 cut(s) 614, 1445
Eco91I GGTNACC 4 cut(s) 337, 509, 1168, 1340
EcoO65I GGTNACC 4 cut(s) 337, 509, 1168, 1340
EcoRII CCWGG 2 cut(s) 513, 1344
EcoRV GATATC 2 cut(s) 75, 906
EcoT14I CCWWGG 2 cut(s) 865, 1696
ErhI CCWWGG 2 cut(s) 865, 1696
Esp3I CGTCTC 2 cut(s) 179, 1010
FaeI CATG 6 cut(s) 181, 352, 395, 1012, 1183, 1226
FaqI GGGAC 2 cut(s) 430, 1261
FatI CATG 6 cut(s) 177, 348, 391, 1008, 1179, 1222
FauNDI CATATG 2 cut(s) 100, 931
FbaI TGATCA 1 cut(s) 1705
FokI GGATG 4 cut(s) 609, 751, 1440, 1582
GsaI CCCAGC 2 cut(s) 578, 1409
HaeIII GGCC 6 cut(s) 235, 353, 827, 1066, 1184, 1658
HapII CCGG 9 cut(s) 68, 141, 332, 561, 899, 972, 1163, 1392, 1796
Hin1II CATG 6 cut(s) 181, 352, 395, 1012, 1183, 1226
HincII GTYRAC 6 cut(s) 378, 486, 537, 1209, 1317, 1368
HindII GTYRAC 6 cut(s) 378, 486, 537, 1209, 1317, 1368
HindIII AAGCTT 6 cut(s) 264, 407, 698, 1095, 1238, 1529
HinfI GANTC 6 cut(s) 148, 328, 979, 1159, 1752, 1760
HpaII CCGG 9 cut(s) 68, 141, 332, 561, 899, 972, 1163, 1392, 1796
Hpy166II GTNNAC 8 cut(s) 378, 486, 537, 767, 1209, 1317, 1368, 1598
Hpy188I TCNGA 4 cut(s) 327, 596, 1158, 1427
Hpy188III TCNNGA 5 cut(s) 289, 720, 1120, 1551, 1709
Hpy8I GTNNAC 8 cut(s) 378, 486, 537, 767, 1209, 1317, 1368, 1598
HpyAV CCTTC 9 cut(s) 352, 394, 463, 509, 1183, 1225, 1294, 1340, 1819
HpyCH4III ACNGT 8 cut(s) 173, 421, 534, 604, 1004, 1252, 1365, 1435
HpyCH4IV ACGT 2 cut(s) 539, 1370
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 317, 401, 968, 1148, 1232, 1727, 1730, 1790
HpyF3I CTNAG 1 cut(s) 1736
HpySE526I ACGT 2 cut(s) 539, 1370
Hsp92II CATG 6 cut(s) 181, 352, 395, 1012, 1183, 1226
Ksp22I TGATCA 1 cut(s) 1705
Kzo9I GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
LmnI GCTCC 2 cut(s) 669, 1500
LweI GCATC 2 cut(s) 587, 1418
MaeII ACGT 2 cut(s) 539, 1370
MaeIII GTNAC 9 cut(s) 337, 509, 566, 727, 1168, 1340, 1397, 1558, 1814
MalI GATC 6 cut(s) 86, 446, 917, 1277, 1707, 1741
MbiI CCGCTC 2 cut(s) 808, 1639
MboI GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
MboII GAAGA 5 cut(s) 288, 494, 1119, 1325, 1734
MflI RGATCY 1 cut(s) 1739
MlsI TGGCCA 4 cut(s) 353, 827, 1184, 1658
MluCI AATT 8 cut(s) 93, 680, 723, 924, 1511, 1554, 1766, 1776
MluNI TGGCCA 4 cut(s) 353, 827, 1184, 1658
MlyI GAGTC 1 cut(s) 1746
MmeI TCCRAC 4 cut(s) 348, 668, 1179, 1499
Mox20I TGGCCA 4 cut(s) 353, 827, 1184, 1658
MscI TGGCCA 4 cut(s) 353, 827, 1184, 1658
MseI TTAA 4 cut(s) 59, 860, 890, 1691
Msp20I TGGCCA 4 cut(s) 353, 827, 1184, 1658
MspA1I CMGCKG 2 cut(s) 710, 1541
MspI CCGG 9 cut(s) 68, 141, 332, 561, 899, 972, 1163, 1392, 1796
MspR9I CCNGG 2 cut(s) 515, 1346
MvaI CCWGG 2 cut(s) 515, 1346
MwoI GCNNNNNNNGC 9 cut(s) 137, 317, 401, 968, 1148, 1232, 1727, 1730, 1790
NdeI CATATG 2 cut(s) 100, 931
NdeII GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
NlaIII CATG 6 cut(s) 181, 352, 395, 1012, 1183, 1226
NlaIV GGNNCC 2 cut(s) 671, 1502
NmuCI GTSAC 6 cut(s) 337, 509, 727, 1168, 1340, 1814
PcsI WCGNNNNNNNCGW 2 cut(s) 600, 1431
PfeI GAWTC 5 cut(s) 148, 328, 979, 1159, 1760
PinAI ACCGGT 2 cut(s) 560, 1391
PleI GAGTC 1 cut(s) 1746
PpsI GAGTC 1 cut(s) 1746
Psp6I CCWGG 2 cut(s) 513, 1344
PspEI GGTNACC 4 cut(s) 337, 509, 1168, 1340
PspFI CCCAGC 2 cut(s) 574, 1405
PspGI CCWGG 2 cut(s) 513, 1344
PspN4I GGNNCC 2 cut(s) 671, 1502
PspPI GGNCC 7 cut(s) 35, 234, 464, 779, 1065, 1295, 1610
PsuI RGATCY 1 cut(s) 1739
PvuII CAGCTG 2 cut(s) 710, 1541
RsaI GTAC 4 cut(s) 255, 768, 1086, 1599
RsaNI GTAC 4 cut(s) 254, 767, 1085, 1598
SaqAI TTAA 4 cut(s) 59, 860, 890, 1691
Sau3AI GATC 6 cut(s) 84, 444, 915, 1275, 1705, 1739
Sau96I GGNCC 7 cut(s) 35, 234, 464, 779, 1065, 1295, 1610
SchI GAGTC 1 cut(s) 1746
ScrFI CCNGG 2 cut(s) 515, 1346
SfaNI GCATC 2 cut(s) 587, 1418
SinI GGWCC 5 cut(s) 35, 464, 779, 1295, 1610
SmlI CTYRAG 1 cut(s) 1788
SmoI CTYRAG 1 cut(s) 1788
Sse9I AATT 8 cut(s) 93, 680, 723, 924, 1511, 1554, 1766, 1776
SspI AATATT 4 cut(s) 157, 798, 988, 1629
StyD4I CCNGG 2 cut(s) 513, 1344
StyI CCWWGG 2 cut(s) 865, 1696
TaaI ACNGT 8 cut(s) 173, 421, 534, 604, 1004, 1252, 1365, 1435
TaiI ACGT 2 cut(s) 542, 1373
TaqI TCGA 8 cut(s) 77, 219, 299, 505, 908, 1050, 1130, 1336
TasI AATT 8 cut(s) 93, 680, 723, 924, 1511, 1554, 1766, 1776
TatI WGTACW 2 cut(s) 253, 1084
TauI GCSGC 3 cut(s) 521, 1352, 1724
TfiI GAWTC 5 cut(s) 148, 328, 979, 1159, 1760
Tru1I TTAA 4 cut(s) 59, 860, 890, 1691
Tru9I TTAA 4 cut(s) 59, 860, 890, 1691
TscAI CASTG 8 cut(s) 288, 322, 426, 680, 1119, 1153, 1257, 1511
TseFI GTSAC 6 cut(s) 337, 509, 727, 1168, 1340, 1814
Tsp45I GTSAC 6 cut(s) 337, 509, 727, 1168, 1340, 1814
TspRI CASTG 8 cut(s) 288, 322, 426, 680, 1119, 1153, 1257, 1511
VpaK11BI GGWCC 5 cut(s) 35, 464, 779, 1295, 1610
XapI RAATTY 3 cut(s) 93, 924, 1776
XmaJI CCTAGG 2 cut(s) 865, 1696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.